Lsi04G016470 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi04G016470
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionCytochrome P450 family 709 protein
Locationchr04 : 23946285 .. 23948961 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATATATGGGTTGGGAGAAACCCAAAATCCAATCACAGAACTTCCACTACTTCTCTAATTAAGCTTTGAATTTTAAAATTTTCCAATGTATATTATTGTGCCTTTCGTTCTTCTTTTTGTCTCAATCCTCTTCAAATTTCTCTACGCCAATTTATGGGTTCCATGGAGAATTCAAATCCACTTCCAAAAACAGGGGATAACAGGGCCTTCCTACCGTCCGATTATCGGTAACGCCACCGATATCCGCCGTCTGTACAAGGAGGCCAATGCGAAGCCAATTCCGTTAACCCACGATATAGTGTGTCGGGTGCTGCCTTATATTCACCGGTGGTCCGAGGAGTACGGAAAGATGTTTGTGTATTGGTTCGGGTCGAAACCGAGATTGGCGATATCCGACCCGGAAATGATTAAGGAAGTGCTTACGAATACGGGTGGGCCTTTTAGAAAGGTCGGGTTCACTCCGGTTTCCAAGTTGCTGTTTGGAGAAGGACTTGTCGGACTCGAGGGTGAACAATGGGTCCTCCATCGGAAAATCGCTAATCAGGCTTTCACCATCGACCGTGTGAAGGTTTTAAAACAAAACCGACCCTTTTTTTTTTCTTTCTACTACACATTTAACTTTTATTTTATTTTGTGTTTTTTTTAACTCTAACTTCAAAACAATTACGACCAAGTTAGTATTCTTTTATAAACTTTCGAAAAATAGATATGATCAGGTGCAACCTAGTTTCATGCTTCCCTTTTCTTTATACATATAAAAATATTATTTTTTCTAAAAAAAATATTTGCCACAATTTAGACAACCGCCTAGGCTGCACAAAATTAAGTATAGCACTAGCAGTACCTAAATTTTTCACTTTGAAAAATAATACTATAAACATATTTTTCTTGTTTATGTCTTTTTTTTTTGTCGCGTATTTTTTAAAATAAAAAGATTGTTTTTTAAAATCCTACAAATAACATAGTTTTGAAGAACCAACAAAACTTTTTTTAGGTCAACGACATGTGGGGATGAGAGATCTGAACCTCTAATATTTTTTAAAAAAATTATTTTTTTAATATTTATAAATAGTTTGACTCATTTTTTATATTTGAAAATATCCATTTTTTTTAAATAAATATTTAAATTTCTAGTCAAATTAAAAAATAAAAAAAAACAATTTTTTTTAGTTTTCAAAACTTTGCTATATATTTCCAAAACAAAAAATACAAAAAAAGAAAGAAAGAAAAGAAAAAAATCAATAAGTCGTGTTTATAGTCTTAAATTTAAAAATATTATTGAATGAAGTAGCTTATTTTTTTTTTTCTTTTGTTCTAAATTAAAAATTATGGCAAAGTATTTAATCCAAAAATCACCAATTCTCAGGGTTGGTTACCAGAGATTACATTGAGTGTTCGTAATGTTTTGGAGAAATGGGAAGAGATGAAGGGAGGACAGGAAGAGTTTGAATTAGATGTCCATAAACAACTTCGCCTCCTCACAGCTGACGTAATTTCAAGAACTGCTTTTGGAAGTAATTTTGAAGAAGGCAAACGTATTTTCAATTTGCAAGAACAACAAATGCATCATTTTCTCCAGGCCGTCTCAAGTGTTTATATTCCAGGCTATAGGTTTATCATTTTCCTAAATTTTTTCGAAAATCATAACCCATTTGCTTAAGTTTCTCAATTCAAATCAACAATTAATATTGAATTTTCACTTGGAAAACCACAGATTTTTACCTACCAAGGGGAACAGAGAGAGGCAGAGACTGGAAAAAGAAACTCGTGCTTCAATAAAAGCACTAGTTGAAAGTGAGAAAAATAGCAAAGAAAGGGAAAACTCAACCAATCTACTTAGTCTCTTATTGTCATCATACAAGAACCAGAATGGAGAAATAGAAAAACTTGGAGTAGAGGAAGTCGTAAATGAATGCAAGACATTCTATTTTGCAGGCATGGAAACAACTGCTAATCTTTTAACTTGGGCCCTTTTACTACTAGCAGAGCATCAAGAATGGCAAGACCGAGCTCGAAAAGAAGTCATCAAAGTCTGCGGCCACAAGACACCGCCTGTGGCCGATAATTTAACCGAACTTAAACTAGTAAGTACTAAATCACCAATCAACTAAAAAACTTAAACTGATATGAGAAACAAGTGTTTAATGATGCGTTTGTTTTGACAGGTGGGTATGATAGTCAACGAAACACTTCGACTTTATCCTCCAGCAGTGATGATGATGCGGCGGACAACAAAACGGTTGACGTTAGGAAACGTCGATGTGCCGGAAGGCACACAACTTCAACTCTCAGTGGCTGCAGTTCACCATGACACAGATCTTTGGGGAGAAGATGCGAGCAGTTTCAACCCTATGAGATTTAGTGAGCCTAGAAAGCATTTGGCTTCATTTTTGCCATTTGGGTTGGGTCCAAGAATCTGTGTAGGCCAACATTTGGCTTTGATTGAGGCCAAAGTTGCATTGGCTATGATAATTCAACGATTCTCTTTTACGGTTTCTCCAACCTACACGCATGCCCCCATGATGTTTGTAAGCTTAAATCCTCAATTCGGTGTTCAGCTCCTCGTTAGGAGCTTATGGAATTGATTATGTGTCTCTGTTCAAATTGTTATCATGGAAATAAATCGGTATCTCTTCTACTTATTTTCATTAATAAATAATTAGTTGTTTCAAATTCA

mRNA sequence

AATATATGGGTTGGGAGAAACCCAAAATCCAATCACAGAACTTCCACTACTTCTCTAATTAAGCTTTGAATTTTAAAATTTTCCAATGTATATTATTGTGCCTTTCGTTCTTCTTTTTGTCTCAATCCTCTTCAAATTTCTCTACGCCAATTTATGGGTTCCATGGAGAATTCAAATCCACTTCCAAAAACAGGGGATAACAGGGCCTTCCTACCGTCCGATTATCGGTAACGCCACCGATATCCGCCGTCTGTACAAGGAGGCCAATGCGAAGCCAATTCCGTTAACCCACGATATAGTGTGTCGGGTGCTGCCTTATATTCACCGGTGGTCCGAGGAGTACGGAAAGATGTTTGTGTATTGGTTCGGGTCGAAACCGAGATTGGCGATATCCGACCCGGAAATGATTAAGGAAGTGCTTACGAATACGGGTGGGCCTTTTAGAAAGGTCGGGTTCACTCCGGTTTCCAAGTTGCTGTTTGGAGAAGGACTTGTCGGACTCGAGGGTGAACAATGGGTCCTCCATCGGAAAATCGCTAATCAGGCTTTCACCATCGACCGTGTGAAGGGTTGGTTACCAGAGATTACATTGAGTGTTCGTAATGTTTTGGAGAAATGGGAAGAGATGAAGGGAGGACAGGAAGAGTTTGAATTAGATGTCCATAAACAACTTCGCCTCCTCACAGCTGACGTAATTTCAAGAACTGCTTTTGGAAGTAATTTTGAAGAAGGCAAACGTATTTTCAATTTGCAAGAACAACAAATGCATCATTTTCTCCAGGCCGTCTCAAGTGTTTATATTCCAGGCTATAGATTTTTACCTACCAAGGGGAACAGAGAGAGGCAGAGACTGGAAAAAGAAACTCGTGCTTCAATAAAAGCACTAGTTGAAAGCATGGAAACAACTGCTAATCTTTTAACTTGGGCCCTTTTACTACTAGCAGAGCATCAAGAATGGCAAGACCGAGCTCGAAAAGAAGTCATCAAAGTCTGCGGCCACAAGACACCGCCTGTGGCCGATAATTTAACCGAACTTAAACTAGTGGGTATGATAGTCAACGAAACACTTCGACTTTATCCTCCAGCAGTGATGATGATGCGGCGGACAACAAAACGGTTGACGTTAGGAAACGTCGATGTGCCGGAAGGCACACAACTTCAACTCTCAGTGGCTGCAGTTCACCATGACACAGATCTTTGGGGAGAAGATGCGAGCAGTTTCAACCCTATGAGATTTAGTGAGCCTAGAAAGCATTTGGCTTCATTTTTGCCATTTGGGTTGGGTCCAAGAATCTGTGTAGGCCAACATTTGGCTTTGATTGAGGCCAAAGTTGCATTGGCTATGATAATTCAACGATTCTCTTTTACGGTTTCTCCAACCTACACGCATGCCCCCATGATGTTTGTAAGCTTAAATCCTCAATTCGGTGTTCAGCTCCTCGTTAGGAGCTTATGGAATTGATTATGTGTCTCTGTTCAAATTGTTATCATGGAAATAAATCGGTATCTCTTCTACTTATTTTCATTAATAAATAATTAGTTGTTTCAAATTCA

Coding sequence (CDS)

ATGTATATTATTGTGCCTTTCGTTCTTCTTTTTGTCTCAATCCTCTTCAAATTTCTCTACGCCAATTTATGGGTTCCATGGAGAATTCAAATCCACTTCCAAAAACAGGGGATAACAGGGCCTTCCTACCGTCCGATTATCGGTAACGCCACCGATATCCGCCGTCTGTACAAGGAGGCCAATGCGAAGCCAATTCCGTTAACCCACGATATAGTGTGTCGGGTGCTGCCTTATATTCACCGGTGGTCCGAGGAGTACGGAAAGATGTTTGTGTATTGGTTCGGGTCGAAACCGAGATTGGCGATATCCGACCCGGAAATGATTAAGGAAGTGCTTACGAATACGGGTGGGCCTTTTAGAAAGGTCGGGTTCACTCCGGTTTCCAAGTTGCTGTTTGGAGAAGGACTTGTCGGACTCGAGGGTGAACAATGGGTCCTCCATCGGAAAATCGCTAATCAGGCTTTCACCATCGACCGTGTGAAGGGTTGGTTACCAGAGATTACATTGAGTGTTCGTAATGTTTTGGAGAAATGGGAAGAGATGAAGGGAGGACAGGAAGAGTTTGAATTAGATGTCCATAAACAACTTCGCCTCCTCACAGCTGACGTAATTTCAAGAACTGCTTTTGGAAGTAATTTTGAAGAAGGCAAACGTATTTTCAATTTGCAAGAACAACAAATGCATCATTTTCTCCAGGCCGTCTCAAGTGTTTATATTCCAGGCTATAGATTTTTACCTACCAAGGGGAACAGAGAGAGGCAGAGACTGGAAAAAGAAACTCGTGCTTCAATAAAAGCACTAGTTGAAAGCATGGAAACAACTGCTAATCTTTTAACTTGGGCCCTTTTACTACTAGCAGAGCATCAAGAATGGCAAGACCGAGCTCGAAAAGAAGTCATCAAAGTCTGCGGCCACAAGACACCGCCTGTGGCCGATAATTTAACCGAACTTAAACTAGTGGGTATGATAGTCAACGAAACACTTCGACTTTATCCTCCAGCAGTGATGATGATGCGGCGGACAACAAAACGGTTGACGTTAGGAAACGTCGATGTGCCGGAAGGCACACAACTTCAACTCTCAGTGGCTGCAGTTCACCATGACACAGATCTTTGGGGAGAAGATGCGAGCAGTTTCAACCCTATGAGATTTAGTGAGCCTAGAAAGCATTTGGCTTCATTTTTGCCATTTGGGTTGGGTCCAAGAATCTGTGTAGGCCAACATTTGGCTTTGATTGAGGCCAAAGTTGCATTGGCTATGATAATTCAACGATTCTCTTTTACGGTTTCTCCAACCTACACGCATGCCCCCATGATGTTTGTAAGCTTAAATCCTCAATTCGGTGTTCAGCTCCTCGTTAGGAGCTTATGGAATTGA

Protein sequence

MYIIVPFVLLFVSILFKFLYANLWVPWRIQIHFQKQGITGPSYRPIIGNATDIRRLYKEANAKPIPLTHDIVCRVLPYIHRWSEEYGKMFVYWFGSKPRLAISDPEMIKEVLTNTGGPFRKVGFTPVSKLLFGEGLVGLEGEQWVLHRKIANQAFTIDRVKGWLPEITLSVRNVLEKWEEMKGGQEEFELDVHKQLRLLTADVISRTAFGSNFEEGKRIFNLQEQQMHHFLQAVSSVYIPGYRFLPTKGNRERQRLEKETRASIKALVESMETTANLLTWALLLLAEHQEWQDRARKEVIKVCGHKTPPVADNLTELKLVGMIVNETLRLYPPAVMMMRRTTKRLTLGNVDVPEGTQLQLSVAAVHHDTDLWGEDASSFNPMRFSEPRKHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQRFSFTVSPTYTHAPMMFVSLNPQFGVQLLVRSLWN
BLAST of Lsi04G016470 vs. Swiss-Prot
Match: C734A_ARATH (Cytochrome P450 734A1 OS=Arabidopsis thaliana GN=CYP734A1 PE=2 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 1.8e-94
Identity = 187/509 (36.74%), Postives = 291/509 (57.17%), Query Frame = 1

Query: 5   VPFVLLFVSILFKFLYANL----WVPWRIQIHFQKQGITGPSYRPIIGNATDIRRLYKEA 64
           +P VL+   IL   +   +    W P +I+ HF KQGI GP Y   IGN  ++  +  +A
Sbjct: 10  IPKVLVLSVILSLVIVKGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKA 69

Query: 65  NAKPIPLTHDIVCRVLPYIHRWSEEYGKMFVYWFGSKPRLAISDPEMIKEVLTNTGGPFR 124
           ++ P+P +H+I+ RVL + H W + YG  F+ WFG   RL ++DP++I+E+ + +   + 
Sbjct: 70  SSHPMPFSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIFSKSEF-YE 129

Query: 125 KVGFTPVSKLLFGEGLVGLEGEQWVLHRKIANQAFTIDRVKGWLPEITLSVRNVLEKWEE 184
           K    P+ K L G+GL+ L+GE+W  HRKI +  F ++ +K  +P +  SV ++++KW +
Sbjct: 130 KNEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSD 189

Query: 185 MKGGQEEFELDVHKQLRLLTADVISRTAFGSNFEEGKRIFNLQEQQMHHFLQAVSS---- 244
                 E E+DV++  ++LT DVISRTAFGS++E+G+ +F LQ QQM    +A       
Sbjct: 190 KLSENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFIP 249

Query: 245 ---------------------------VYIPGYRFLPTKGNRERQRLEKET--------R 304
                                      +       +  +G   ++   K+          
Sbjct: 250 GYRFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMIQAKN 309

Query: 305 ASIKALVESM--------ETTANLLTWALLLLAEHQEWQDRARKEVIKVCGHKTPPVADN 364
            +++ +VE          +TT+NLLTW  +LL+ H EWQ +AR EV++VCG +  P  D+
Sbjct: 310 VTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCGSRDVPTKDH 369

Query: 365 LTELKLVGMIVNETLRLYPPAVMMMRRTTKRLTLGNVDVPEGTQLQLSVAAVHHDTDLWG 424
           + +LK + MI+NE+LRLYPP V  +RR    + LG   +P GT+L + + AVHHD  +WG
Sbjct: 370 VVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPIIAVHHDQAIWG 429

Query: 425 EDASSFNPMRFSE--PR--KHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQRFSFTV 459
            D + FNP RF++  PR  KH   F+PFGLG R C+GQ+LA+++AK+ LA++IQRF+F +
Sbjct: 430 NDVNEFNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLTLAVMIQRFTFHL 489

BLAST of Lsi04G016470 vs. Swiss-Prot
Match: C7345_ORYSJ (Cytochrome P450 734A5 OS=Oryza sativa subsp. japonica GN=CYP734A5 PE=2 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 1.4e-91
Identity = 184/492 (37.40%), Postives = 274/492 (55.69%), Query Frame = 1

Query: 24  WVPWRIQIHFQKQGITGPSYRPIIGNATDIRRLYKEANAKPI--PLTHDIVCRVLPYIHR 83
           W P R++ HF  QG+ GP YR  +G++ ++ RL  +A ++P+  P +HDI+ RVLP+ H 
Sbjct: 41  WRPRRVERHFAAQGVRGPGYRFFVGSSIELVRLMVDAASRPMEPPTSHDILPRVLPFYHH 100

Query: 84  WSEEYGKMFVYWFGSKPRLAISDPEMIKEVLTNTGGPFRKVGFTPVSKLLFGEGLVGLEG 143
           W + YG M + WFG  PRL +S+PE+I+EVL      F +    P+     G GL  L G
Sbjct: 101 WRKLYGPMHLIWFGRTPRLVVSEPELIREVLLTRADHFDRYEAHPMICQFEGYGLSNLHG 160

Query: 144 EQWVLHRKIANQAFTIDRVKGWLPEITLSVRNVLEKWEEMK--GGQEEFELDVHKQLRLL 203
           E+W   R++   AF  + ++   P +  +V  +L++  E    GG  E E+DV +  + +
Sbjct: 161 ERWARRRRVLTPAFHTENLRMIAPFVAGTVTRMLDELAERARAGGAGEAEVDVAEWFQRV 220

Query: 204 TADVISRTAFGS-NFEEGKRIFNLQEQQMHHFLQAVSSVYIPG----------------- 263
             + I+  AFG  N+++G  +F LQ++   +  +A S VYIPG                 
Sbjct: 221 PQEAITFAAFGRRNYDDGAAVFRLQDELAGYATEAHSKVYIPGYRFLPTRKNRRVWQLDR 280

Query: 264 ------YRFLPTK-------------------GNRERQRLEKETRASIKALVESM----- 323
                  +F+                      G RE          + + + ES      
Sbjct: 281 EIRSHLAKFVTGLQSCSSSHGDDADDGGDGGGGMREFMSFMAPAMTAGEIIEESKNFFFA 340

Query: 324 --ETTANLLTWALLLLAEHQEWQDRARKEVIKVCGHKTPPVADNLTELKLVGMIVNETLR 383
             ET +NLLTW  + LA H EWQ+RAR+EV+ VCG    P  D+L +LK +GMI+NETLR
Sbjct: 341 GKETLSNLLTWTTVALAMHPEWQERARREVVAVCGRGDLPTKDHLPKLKTLGMILNETLR 400

Query: 384 LYPPAVMMMRRTTKRLTLGNVDVPEGTQLQLSVAAVHHDTDLWGEDASSFNPMRFSEP-- 443
           LYPPAV M+R   + + LG   VP GT++ + + AVHHD   WG+DA+ FNP RF+    
Sbjct: 401 LYPPAVAMIRTAKEDVELGGCVVPAGTEVMIPIMAVHHDAAAWGDDAAEFNPARFAADDD 460

Query: 444 ---RKHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQRFSFTVSPTYTHAPMMFVSLN 457
              R+H  +F+PFG G R+C+GQ++AL+EAKVALA++++RF F +SP Y HAP + + L+
Sbjct: 461 GGRRRHPMAFMPFGGGARVCIGQNMALMEAKVALAVVLRRFEFRLSPAYVHAPRVLMILS 520

BLAST of Lsi04G016470 vs. Swiss-Prot
Match: C7A15_ARATH (Cytochrome P450 72A15 OS=Arabidopsis thaliana GN=CYP72A15 PE=2 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 2.4e-91
Identity = 186/489 (38.04%), Postives = 277/489 (56.65%), Query Frame = 1

Query: 24  WV---PWRIQIHFQKQGITGPSYRPIIGNATDIRRLYKEANAKPIPLTHDIVCRVLPYIH 83
           WV   P  ++ + ++QG+ G  Y P++G+      +  EA +KP+ LT DI  RV+PY  
Sbjct: 27  WVWFKPKMLEHYLRRQGLAGTPYTPLVGDLKKNFTMLSEARSKPLKLTDDISPRVVPYPL 86

Query: 84  RWSEEYGKMFVYWFGSKPRLAISDPEMIKEVLTNTGGPFRKVGFTPVSKLLFGEGLVGLE 143
           +  + YG+ +  WFG  P + I DPE IKEV  N    F+K    P++ ++  +GL   +
Sbjct: 87  QMFKTYGRTYFTWFGPIPTITIMDPEQIKEVF-NKVYDFQKPHTFPLATII-AKGLANYD 146

Query: 144 GEQWVLHRKIANQAFTIDRVKGWLPEITLSVRNVLEKWEEMKGGQ-EEFELDVHKQLRLL 203
           G++W  HR+I N AF I+++K  +P    S R V+ +W+++   +    E+DV   L  +
Sbjct: 147 GDKWAKHRRIINPAFHIEKIKNMVPAFHQSCREVVGEWDQLVSDKGSSCEVDVWPGLVSM 206

Query: 204 TADVISRTAFGSNFEEGKRIFNLQEQQMHHFLQAVSSVYIPGYRF--------------- 263
           TADVISRTAFGS+++EG+RIF LQ +     +QA    +IPGY +               
Sbjct: 207 TADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKAFIPGYSYLPTKSNRRMKAAARE 266

Query: 264 -------LPTKGNRERQRLEKETRASIKALVES--------------------------M 323
                  +  K  R R+  E  +   +  L+ES                           
Sbjct: 267 IQVILRGIVNKRLRAREAGEAPSDDLLGILLESNLRQTEGNGMSTEDLMEECKLFYFAGQ 326

Query: 324 ETTANLLTWALLLLAEHQEWQDRARKEVIKVCGHKTPPVADNLTELKLVGMIVNETLRLY 383
           ETT+ LL W ++LL++HQ+WQ RAR+EV +V G K P  A+ L +LK++ MI+ E LRLY
Sbjct: 327 ETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEPD-AEGLNQLKVMTMILYEVLRLY 386

Query: 384 PPAVMMMRRTTKRLTLGNVDVPEGTQLQLSVAAVHHDTDLWGEDASSFNPMRF----SEP 443
           PP   + R   K L LG++ +P G Q+ L +  V HD +LWG DA+ FNP RF    S+ 
Sbjct: 387 PPVTQLTRAIHKELKLGDLTLPGGVQISLPILLVQHDIELWGNDAAEFNPDRFKDGLSKA 446

Query: 444 RKHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQRFSFTVSPTYTHAPMMFVSLNPQF 457
            K   SF PF  GPRIC+GQ+ AL+EAK+A+A+I++RFSF +SP+Y HAP   ++++PQF
Sbjct: 447 TKSQVSFFPFAWGPRICIGQNFALLEAKMAMALILRRFSFEISPSYVHAPYTVITIHPQF 506

BLAST of Lsi04G016470 vs. Swiss-Prot
Match: C7A14_ARATH (Cytochrome P450 72A14 OS=Arabidopsis thaliana GN=CYP72A14 PE=2 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 4.4e-85
Identity = 181/486 (37.24%), Postives = 269/486 (55.35%), Query Frame = 1

Query: 24  WVPWRIQIHFQKQGITGPSYRPIIGNATDIRRLYKEANAKPIPLTHDIVCRVLPYIHRWS 83
           + P  ++   ++QG++G SY P+IG+   +  ++ EA +KPI  T DI  RV+P+  +  
Sbjct: 30  FTPKMLERSLRRQGLSGTSYTPLIGDFKKMISMFIEATSKPIKPTDDITPRVMPHPLQML 89

Query: 84  EEYGKMFVYWFGSKPRLAISDPEMIKEVLTNTGGPFRKVGFTPVSKLLFGEGLVGLEGEQ 143
           + +G+  + WFG  P + I DPE IKEV  N    F+K    P+SK+L G GLV  +G++
Sbjct: 90  KTHGRTNLTWFGPIPTITIMDPEQIKEVF-NKVYDFQKAHTFPLSKIL-GTGLVSYDGDK 149

Query: 144 WVLHRKIANQAFTIDRVKGWLPEITLSVRNVLEKWEEMKGGQ-EEFELDVHKQLRLLTAD 203
           W  HR+I N AF ++++K  +     S   ++ +W+++   +    E+DV   L  +TAD
Sbjct: 150 WAQHRRIINPAFHLEKIKNMVHVFHESCSELVGEWDKLVSDKGSSCEVDVWPGLTSMTAD 209

Query: 204 VISRTAFGSNFEEGKRIFNLQEQQMHHFLQA----------------------VSSVYIP 263
           VISRTAFGS++ EG RIF LQ +     +QA                       +     
Sbjct: 210 VISRTAFGSSYREGHRIFELQAELAQLVMQAFQKFFIPGYIYLPTKGNRRMKTAAREIQD 269

Query: 264 GYRFLPTKGNRERQRLEKETRASIKALVES--------------------------METT 323
             R +  K  R R+  E  +   +  L+ES                           ETT
Sbjct: 270 ILRGIINKRERARESGEAPSEDLLGILLESNLGQTEGNGMSTEDMMEECKLFYLAGQETT 329

Query: 324 ANLLTWALLLLAEHQEWQDRARKEVIKVCGHKTPPVADNLTELKLVGMIVNETLRLYPPA 383
           + LL W ++LL++HQ+WQ RAR+EV +V G K P   + L +LK++ MI+ E LRLYPP 
Sbjct: 330 SVLLVWTMVLLSQHQDWQARAREEVKQVFGDKQPDT-EGLNQLKVMTMILYEVLRLYPPV 389

Query: 384 VMMMRRTTKRLTLGNVDVPEGTQLQLSVAAVHHDTDLWGEDASSFNPMRF----SEPRKH 443
           V + R   K + LG++ +P G Q+ L V  VH DT+LWG DA  F P RF    S+  K+
Sbjct: 390 VQLTRAIHKEMKLGDLTLPGGVQISLPVLLVHRDTELWGNDAGEFKPERFKDGLSKATKN 449

Query: 444 LASFLPFGLGPRICVGQHLALIEAKVALAMIIQRFSFTVSPTYTHAPMMFVSLNPQFGVQ 457
             SF PF  GPRIC+GQ+  L+EAK+A+++I+QRFSF +SP+Y HAP   ++L PQFG  
Sbjct: 450 QVSFFPFAWGPRICIGQNFTLLEAKMAMSLILQRFSFELSPSYVHAPYTIITLYPQFGAH 509

BLAST of Lsi04G016470 vs. Swiss-Prot
Match: C7A13_ARATH (Cytochrome P450 72A13 OS=Arabidopsis thaliana GN=CYP72A13 PE=2 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 2.9e-84
Identity = 177/488 (36.27%), Postives = 269/488 (55.12%), Query Frame = 1

Query: 23  LWV-PWRIQIHFQKQGITGPSYRPIIGNATDIRRLYKEANAKPIPLTHDIVCRVLPYIHR 82
           +W+ P  ++ + ++QG+ G  Y P++G+      +  EA +KPI LT DI  R++PY  +
Sbjct: 28  VWLKPKMLESYLRRQGLAGTPYTPLVGDLKRNFSMLAEARSKPINLTDDITPRIVPYPLQ 87

Query: 83  WSEEYGKMFVYWFGSKPRLAISDPEMIKEVLTNTGGPFRKVGFTPVSKLLFGEGLVGLEG 142
             + +G+ F  WFG  P + I DPE IKEV  N    F+K    P+ +L+   GLV  +G
Sbjct: 88  MLKTHGRTFFTWFGPIPTITIMDPEQIKEVF-NKVYDFQKAHTFPLGRLI-AAGLVSYDG 147

Query: 143 EQWVLHRKIANQAFTIDRVKGWLPEITLSVRNVLEKWEEM-KGGQEEFELDVHKQLRLLT 202
           ++W  HR+I N AF ++++K  +P    S   ++ +W+++    Q   E+D+   L  +T
Sbjct: 148 DKWTKHRRIINPAFHLEKIKNMVPAFHQSCSEIVGEWDKLVTDKQSSCEVDIWPWLVSMT 207

Query: 203 ADVISRTAFGSNFEEGKRIFNLQEQQMHHFLQAVSSV------YIPG------------- 262
           ADVISRTAFGS+++EG+RIF LQ +     +QA          Y P              
Sbjct: 208 ADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKAIIPGYRYFPTKGNRRMKAAAREI 267

Query: 263 ---YRFLPTKGNRERQRLEKETRASIKALVES--------------------------ME 322
               R +  K  R R+  E  +   +  L+ES                           E
Sbjct: 268 KFILRGIVNKRLRAREAGEAPSDDLLGILLESNLGQTKGNGMSTEELMEECKLFYFAGQE 327

Query: 323 TTANLLTWALLLLAEHQEWQDRARKEVIKVCGHKTPPVADNLTELKLVGMIVNETLRLYP 382
           TT  LL W ++LL++HQ+WQ RAR+EV +V G K P  A+ L +LK++ MI+ E LRLYP
Sbjct: 328 TTTVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEPD-AEGLNQLKVMTMILYEVLRLYP 387

Query: 383 PAVMMMRRTTKRLTLGNVDVPEGTQLQLSVAAVHHDTDLWGEDASSFNPMRF----SEPR 442
           P V + R   K + LG++ +P G Q+ L +  +  D +LWG DA  F P RF    S+  
Sbjct: 388 PVVQLTRAIHKEMQLGDLTLPGGVQISLPILLIQRDRELWGNDAGEFKPDRFKDGLSKAT 447

Query: 443 KHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQRFSFTVSPTYTHAPMMFVSLNPQFG 457
           K+  SF PF  GPRIC+GQ+ AL+EAK+A+ +I+++FSF +SP+Y HAP   ++ +PQFG
Sbjct: 448 KNQVSFFPFAWGPRICIGQNFALLEAKMAMTLILRKFSFELSPSYVHAPYTVLTTHPQFG 507

BLAST of Lsi04G016470 vs. TrEMBL
Match: A0A0K9QQC9_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_153300 PE=3 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 1.0e-152
Identity = 267/513 (52.05%), Postives = 348/513 (67.84%), Query Frame = 1

Query: 3   IIVPFVLLFVS--ILFKFLYANLWVPWRIQIHFQKQGITGPSYRPIIGNATDIRRLYKEA 62
           +++PF++L +   IL K  +  LWVPW IQ HF KQGI GP+YRPI GN   IR+LY EA
Sbjct: 5   LLLPFLVLIICTLILLKLTHTLLWVPWSIQRHFNKQGIVGPTYRPIFGNTGQIRQLYSEA 64

Query: 63  NAK------------PIPLTHDIVCRVLPYIHRWSEEYGKMFVYWFGSKPRLAISDPEMI 122
            +K            P+ + HDI  RV+P+ H+WS  YG  F+YWFG KP LAISDP MI
Sbjct: 65  QSKTIQIFPGGPGEDPLVINHDITHRVMPFYHKWSAIYGNTFLYWFGPKPMLAISDPLMI 124

Query: 123 KEVLTNTGGPFRKVGFTPVSKLLFGEGLVGLEGEQWVLHRKIANQAFTIDRVKGWLPEIT 182
           KEVL N    F+K+ + P++K+LFG+GLVGL+G+ W  HR+IANQAF+++RVK W+P+I 
Sbjct: 125 KEVLINNNVLFKKLKYNPLAKMLFGQGLVGLDGDHWAFHRRIANQAFSMERVKSWIPDIV 184

Query: 183 LSVRNVLEKWEEMKGGQEEFELDVHKQLRLLTADVISRTAFGSNFEEGKRIFNLQEQQMH 242
            S R  L +WEE KG QEEFE++VHK+L  L+AD+ISRTAFGS++EEGKRIF LQEQQMH
Sbjct: 185 ASTRKTLIEWEETKGSQEEFEMEVHKELHNLSADIISRTAFGSSYEEGKRIFELQEQQMH 244

Query: 243 HFLQAVSSVYIPGYRFLPTKGNRERQRLEKETRASIKALVES------------------ 302
               A+ S+YIPG+RFLPT  N+ER RL+KETR SIK L+                    
Sbjct: 245 LVSLALRSIYIPGFRFLPTAKNKERWRLDKETRDSIKLLMTKNSETTRNKSNLLSLFMSA 304

Query: 303 ---------------------------METTANLLTWALLLLAEHQEWQDRARKEVIKVC 362
                                       ETTANLLTWAL++L+ HQEWQ++AR EV +VC
Sbjct: 305 TENEGGKVKRLSIDEVVEECKTFYFAGKETTANLLTWALIMLSAHQEWQNKARDEVFQVC 364

Query: 363 GHKTPPVADNLTELKLVGMIVNETLRLYPPAVMMMRRTTKRLTLGNVDVPEGTQLQLSVA 422
           G    P  DN+ + KL+ MIVNETLRLYPPAVMM RR  K + LGN+ +P  T L L++ 
Sbjct: 365 GVHGLPTVDNINDFKLIKMIVNETLRLYPPAVMMKRRAGKEVKLGNLRIPAETLLYLAMT 424

Query: 423 AVHHDTDLWGEDASSFNPMRFSEPRKHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQ 457
            VHHD ++WGEDA+ FNP+RF EPRKH A+F PF LGPR+CVGQ+LA+IE+ + LA+I++
Sbjct: 425 NVHHDVNVWGEDANKFNPLRFLEPRKHPATFFPFSLGPRVCVGQNLAMIESIIVLAIILR 484

BLAST of Lsi04G016470 vs. TrEMBL
Match: A0A072U2N1_MEDTR (Cytochrome P450 family 709 protein OS=Medicago truncatula GN=MTR_7g098550 PE=3 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 2.5e-151
Identity = 263/491 (53.56%), Postives = 353/491 (71.89%), Query Frame = 1

Query: 3   IIVPFVLLFVSILFKFLYANLWVPWRIQIHFQKQGITGPSYRPIIGNATDIRRLYKEANA 62
           ++   VL+ +  L K L++  +VP++IQ HF+ QGI+GP+YRPI GN+++I+RLY E  +
Sbjct: 7   LVTILVLVVLYYLLKTLHSIFYVPYKIQQHFRNQGISGPTYRPIFGNSSEIKRLYAETKS 66

Query: 63  KPIPLTHDIVCRVLPYIHRWSEEYGKMFVYWFGSKPRLAISDPEMIKEVLTNTGGPFRKV 122
           +  P  HDI+ RV+P+ +RWS  YGK F+YWFGS PRLAISDP++IKEVL      + KV
Sbjct: 67  ESNPFDHDILKRVVPFYNRWSCMYGKTFLYWFGSTPRLAISDPDLIKEVLVTNCVEYGKV 126

Query: 123 GFTPVSKLLFGEGLVGLEGEQWVLHRKIANQAFTIDRVKGWLPEITLSVRNVLEKWEEMK 182
            + P SKLLFG+GLVGL+G+QW  HR+I N AF  + +KGW+P+I LSV  +LEKWE  +
Sbjct: 127 PYNPQSKLLFGQGLVGLQGDQWNFHRRIFNLAFNTEILKGWVPDIVLSVTTMLEKWENQR 186

Query: 183 GGQEEFELDVHKQLRLLTADVISRTAFGSNFEEGKRIFNLQEQQMHHFLQAVSSVYIPGY 242
           GG+++FE+DVH+ L  L+ADVISRTAFGS+FEEGK IF LQEQQMH F QAV SVYIPG+
Sbjct: 187 GGRDDFEIDVHRDLHELSADVISRTAFGSSFEEGKHIFKLQEQQMHLFSQAVRSVYIPGF 246

Query: 243 R---------------FLPTKGNRE--------------RQRLEKETRASIKALVESM-- 302
           R                + TK + +              +  +  E +  ++ +++    
Sbjct: 247 RDRWRLDKETRESICKLIETKSSVKENTKNVLNSLMCSYKNEVGGENKLGLEEIIDECKT 306

Query: 303 ------ETTANLLTWALLLLAEHQEWQDRARKEVIKVCGHKTPPVADNLTELKLVGMIVN 362
                 +TTANLL+WALLLLA+HQEWQ  AR+EV++V GH   PVADNL +LK+V MI+N
Sbjct: 307 IYFAGKDTTANLLSWALLLLAKHQEWQSMAREEVLRVIGHSQLPVADNLNDLKIVSMIIN 366

Query: 363 ETLRLYPPAVMMMRRTTKRLTLGNVDVPEGTQLQLSVAAVHHDTDLWGEDASSFNPMRFS 422
           ETLRLYPPA+M+MR+T K + LG+++VP  TQL L +  +HH+ ++WGED   FNPMRFS
Sbjct: 367 ETLRLYPPALMLMRQTNKNVMLGSIEVPAKTQLYLPLTDIHHNREIWGEDCHGFNPMRFS 426

Query: 423 EPRKHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQRFSFTVSPTYTHAPMMFVSLNP 457
           EPRKHLA+F PFGLGPR CVGQ+LAL+EAK+ALA+IIQ +SF VSP+Y HAP++F++L P
Sbjct: 427 EPRKHLAAFFPFGLGPRTCVGQNLALVEAKIALALIIQHYSFEVSPSYIHAPVLFITLQP 486

BLAST of Lsi04G016470 vs. TrEMBL
Match: A0A061FBM4_THECC (Cytochrome P450, family 721, subfamily A, polypeptide 1, putative OS=Theobroma cacao GN=TCM_034002 PE=3 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 2.7e-150
Identity = 266/501 (53.09%), Postives = 354/501 (70.66%), Query Frame = 1

Query: 2   YIIVPFVLLFVSILFKFLYANLWVPWRIQIHFQKQGITGPSYRPIIGNATDIRRLYKEAN 61
           ++++ F+      L KF+Y+ +W+PW+IQ HF+KQG+TGP YRPI GN+ +IRRL+ EA 
Sbjct: 3   HLVLLFIAFLCICLLKFVYSIVWIPWKIQNHFKKQGVTGPGYRPIFGNSAEIRRLFAEAQ 62

Query: 62  AKPIPLTH-DIVCRVLPYIHRWSEEYGKMFVYWFGSKPRLAISDPEMIKEVLTNTGGPFR 121
           +KP PL H D++ RV P+ HRWS  YGK F+YWFGSKPRL ISDP MIKE   NTG  F 
Sbjct: 63  SKPAPLHHHDVLQRVAPFYHRWSNMYGKPFLYWFGSKPRLGISDPAMIKEAAMNTGAAFH 122

Query: 122 KVGFTPVSKLLFGEGLVGLEGEQWVLHRKIANQAFTIDRVKGWLPEITLSVRNVLEKWEE 181
           KVGF P+S++LFG+GLVGLEGE+W +HR+IANQAF+++RVKGW+PEI  +   +LEKWEE
Sbjct: 123 KVGFNPMSRMLFGQGLVGLEGEEWAVHRRIANQAFSMERVKGWVPEIVAATTKMLEKWEE 182

Query: 182 MKGGQEEFELDVHKQLRLLTADVISRTAFGSNFEEGKRIFNLQEQQMHHF------LQAV 241
            + G++EFEL+V+K+L   +ADVISRTAFGS+FEEGKRIF LQEQQM  F      +   
Sbjct: 183 NRAGRDEFELEVNKELHDFSADVISRTAFGSSFEEGKRIFMLQEQQMKLFSLAVRSVYIP 242

Query: 242 SSVYIPGY----RFLPTKGNRE---------------------------RQRLEKETRAS 301
              ++P      R+   K  RE                           + + ++E R  
Sbjct: 243 GFRFLPTRNNRERWRLEKETRESVRALIRANSNRREDSSSLLGLLMSSYKNQHDQEERLG 302

Query: 302 IKALVESM--------ETTANLLTWALLLLAEHQEWQDRARKEVIKVCGHKTPPVADNLT 361
              +++          ET AN L+WALLLLA + EWQD+AR+EV+++CG+   PVA+NL 
Sbjct: 303 EGDIIDECKTFYFAGKETAANALSWALLLLALNPEWQDKAREEVLRICGNDKLPVAENLN 362

Query: 362 ELKLVGMIVNETLRLYPPAVMMMRRTTKRLTLGNVDVPEGTQLQLSVAAVHHDTDLWGED 421
           +LK+V MI+NETLRLYP AV+MMR+  K + LG ++VP GTQL L++ AVH+D D+WGED
Sbjct: 363 DLKIVTMIINETLRLYPAAVLMMRQAGKDVKLGKLNVPTGTQLYLALTAVHNDADIWGED 422

Query: 422 ASSFNPMRFSEPRKHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQRFSFTVSPTYTH 457
           A+ FNP RF E RKHLASFLPFGLGPR CVGQ+LA+ E K+ALAMI++++S  VSPTY H
Sbjct: 423 ANKFNPSRFKESRKHLASFLPFGLGPRTCVGQNLAMAEFKIALAMIVRQYSLAVSPTYVH 482

BLAST of Lsi04G016470 vs. TrEMBL
Match: K7LDC7_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_09G117400 PE=3 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 2.5e-148
Identity = 270/505 (53.47%), Postives = 347/505 (68.71%), Query Frame = 1

Query: 1   MYIIVPFVLLFVSILFKFLYANLWVPWRIQIHFQKQGITGPSYRPIIGNATDIRRLY--- 60
           M +++  V L V    KF Y+N WVPWR + HF++QGI GP YRPI GN ++IRRLY   
Sbjct: 1   MNLLLAAVTLLVFYAAKFFYSNFWVPWRTERHFKRQGIGGPGYRPIFGNTSEIRRLYAEA 60

Query: 61  -KEANAKPIPLTHDIVCRVLPYIHRWSEEYGKMFVYWFGSKPRLAISDPEMIKEVLTNTG 120
             EA+A P P  HDI+ RV P+  RWS  YGK F+YWFGS PRLA+++P+MIKEVL NT 
Sbjct: 61  KSEASASPPPFHHDIMGRVAPFYDRWSRAYGKTFLYWFGSTPRLAVTEPDMIKEVLMNTR 120

Query: 121 GPFRKVGFTPVSKLLFGEGLVGLEGEQWVLHRKIANQAFTIDRVKGWLPEITLSVRNVLE 180
           G + KV F P SKLLFG+GLVGLEG+QW LHR+I N AF ++ VKGW+P+I  SV   LE
Sbjct: 121 GEYVKVPFNPQSKLLFGQGLVGLEGDQWALHRRIINLAFNLELVKGWVPDIVASVTKKLE 180

Query: 181 KWEEMKGGQEEFELDVHKQLRLLTADVISRTAFGSNFEEGKRIFNLQEQQ---------- 240
            WE+ +GG++EFE+DV ++L  L+ADVISRTAFGSN+EEGK IFNLQEQQ          
Sbjct: 181 SWEDQRGGRDEFEIDVLRELHDLSADVISRTAFGSNYEEGKHIFNLQEQQMHLFSQAVRS 240

Query: 241 ----MHHFLQAVSS---------VYIPGYRFLPTKGN-RERQR-------------LEKE 300
                  +L    +               + + TK N RE  R                E
Sbjct: 241 VYIPGFRYLPTKKNKDRWRLEKETRESILKLIETKSNTRENARNVLSSLMCSYKNDAGGE 300

Query: 301 TRASIKALVESM--------ETTANLLTWALLLLAEHQEWQDRARKEVIKVCGHKTPPVA 360
            +  ++ +++          ETTANLLTWALLLLA+HQEWQ +ARKEV+ V G    P A
Sbjct: 301 EKLGVEEIIDECKTIYFAGKETTANLLTWALLLLAKHQEWQSKARKEVLHVIGRNRLPAA 360

Query: 361 DNLTELKLVGMIVNETLRLYPPAVMMMRRTTKRLTLGNVDVPEGTQLQLSVAAVHHDTDL 420
           DNL +LK+V MI+NETLRLYPPAVM+MR+ +K + LG++++P  TQL L++ AVHHD ++
Sbjct: 361 DNLNDLKIVTMIINETLRLYPPAVMLMRQASKDVMLGSINIPAKTQLFLALTAVHHDREI 420

Query: 421 WGEDASSFNPMRFSEPRKHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQRFSFTVSP 457
           WGED  +FNPMRFSEPRKHLA+F PFGLGPRICVGQ+LAL+EAK+ALA+IIQ +SF +SP
Sbjct: 421 WGEDYHNFNPMRFSEPRKHLAAFFPFGLGPRICVGQNLALVEAKIALALIIQSYSFVLSP 480

BLAST of Lsi04G016470 vs. TrEMBL
Match: A0A0L9UTG7_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan06g133900 PE=3 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 2.5e-148
Identity = 265/503 (52.68%), Postives = 351/503 (69.78%), Query Frame = 1

Query: 1   MYIIVPFVLLFVSILFKFLYANLWVPWRIQIHFQKQGITGPSYRPIIGNATDIRRLYKEA 60
           M +++  V+L V    KFLYANLWVP R + HF+KQGI GP Y PI+GN+++IRRLY EA
Sbjct: 1   MNLLLGAVVLLVFFAVKFLYANLWVPRRTERHFKKQGIGGPGYCPIVGNSSEIRRLYAEA 60

Query: 61  NAKPIP-LTHD-IVCRVLPYIHRWSEEYGKMFVYWFGSKPRLAISDPEMIKEVLTNTGGP 120
            ++  P   HD I+ RV+P+  RWS  +GK F+YWFGS PRLAI++P+MIKEVLTNT G 
Sbjct: 61  KSEATPAFEHDNILRRVVPFYERWSRAHGKTFLYWFGSTPRLAITEPDMIKEVLTNTRGE 120

Query: 121 FRKVGFTPVSKLLFGEGLVGLEGEQWVLHRKIANQAFTIDRVKGWLPEITLSVRNVLEKW 180
           + KV + P SKLLFG+GLVGLEGEQW LHR+I N AF ++ VKGW+P+I  SV  ++EKW
Sbjct: 121 YGKVPYNPQSKLLFGQGLVGLEGEQWALHRRIINLAFNLELVKGWVPDIVASVTKMVEKW 180

Query: 181 EEMKGGQEEFELDVHKQLRLLTADVISRTAFGSNFEEGKRIFNLQEQQM----------- 240
           E+ +GG++EFE++VH++L  L+ADVISRTAFGS++EEGK IFNLQEQQM           
Sbjct: 181 EDQRGGKDEFEIEVHRELHDLSADVISRTAFGSSYEEGKHIFNLQEQQMHLFSQAIRSVY 240

Query: 241 -----------------------HHFLQAVSSVYIPGYR-----------FLPTKGNRER 300
                                     L+ + +    G             +    G  E+
Sbjct: 241 IPGFRYLPTRKNRERRRLEKETRESILKLIETKSNTGENARNVLSSLMCSYKNEVGGEEK 300

Query: 301 QRLEKETRASIKALVESMETTANLLTWALLLLAEHQEWQDRARKEVIKVCGHKTPPVADN 360
            R+E+             ETTANLLTW LLLLA+HQEWQ +AR+EV++V GH   PVADN
Sbjct: 301 LRVEEIIDECKTIYFAGKETTANLLTWTLLLLAKHQEWQSKAREEVLRVIGHNQLPVADN 360

Query: 361 LTELKLVGMIVNETLRLYPPAVMMMRRTTKRLTLGNVDVPEGTQLQLSVAAVHHDTDLWG 420
           L++LK+V MI+NETLRLYPPAVM+MR+ +K + LG+++VP  TQL L++ AVHHD ++WG
Sbjct: 361 LSDLKIVSMIINETLRLYPPAVMLMRQASKDVMLGSINVPAKTQLYLALTAVHHDREIWG 420

Query: 421 EDASSFNPMRFSEPRKHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQRFSFTVSPTY 457
           ED  +FNP RFSEPRKHLA+F+PFGLGPR CVGQ+LA++EAK+AL++IIQ +SF +SP Y
Sbjct: 421 EDCHNFNPTRFSEPRKHLAAFIPFGLGPRTCVGQNLAMVEAKIALSLIIQSYSFVLSPNY 480

BLAST of Lsi04G016470 vs. TAIR10
Match: AT2G26710.1 (AT2G26710.1 Cytochrome P450 superfamily protein)

HSP 1 Score: 347.8 bits (891), Expect = 1.0e-95
Identity = 187/509 (36.74%), Postives = 291/509 (57.17%), Query Frame = 1

Query: 5   VPFVLLFVSILFKFLYANL----WVPWRIQIHFQKQGITGPSYRPIIGNATDIRRLYKEA 64
           +P VL+   IL   +   +    W P +I+ HF KQGI GP Y   IGN  ++  +  +A
Sbjct: 10  IPKVLVLSVILSLVIVKGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKA 69

Query: 65  NAKPIPLTHDIVCRVLPYIHRWSEEYGKMFVYWFGSKPRLAISDPEMIKEVLTNTGGPFR 124
           ++ P+P +H+I+ RVL + H W + YG  F+ WFG   RL ++DP++I+E+ + +   + 
Sbjct: 70  SSHPMPFSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIFSKSEF-YE 129

Query: 125 KVGFTPVSKLLFGEGLVGLEGEQWVLHRKIANQAFTIDRVKGWLPEITLSVRNVLEKWEE 184
           K    P+ K L G+GL+ L+GE+W  HRKI +  F ++ +K  +P +  SV ++++KW +
Sbjct: 130 KNEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSD 189

Query: 185 MKGGQEEFELDVHKQLRLLTADVISRTAFGSNFEEGKRIFNLQEQQMHHFLQAVSS---- 244
                 E E+DV++  ++LT DVISRTAFGS++E+G+ +F LQ QQM    +A       
Sbjct: 190 KLSENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFIP 249

Query: 245 ---------------------------VYIPGYRFLPTKGNRERQRLEKET--------R 304
                                      +       +  +G   ++   K+          
Sbjct: 250 GYRFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMIQAKN 309

Query: 305 ASIKALVESM--------ETTANLLTWALLLLAEHQEWQDRARKEVIKVCGHKTPPVADN 364
            +++ +VE          +TT+NLLTW  +LL+ H EWQ +AR EV++VCG +  P  D+
Sbjct: 310 VTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCGSRDVPTKDH 369

Query: 365 LTELKLVGMIVNETLRLYPPAVMMMRRTTKRLTLGNVDVPEGTQLQLSVAAVHHDTDLWG 424
           + +LK + MI+NE+LRLYPP V  +RR    + LG   +P GT+L + + AVHHD  +WG
Sbjct: 370 VVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPIIAVHHDQAIWG 429

Query: 425 EDASSFNPMRFSE--PR--KHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQRFSFTV 459
            D + FNP RF++  PR  KH   F+PFGLG R C+GQ+LA+++AK+ LA++IQRF+F +
Sbjct: 430 NDVNEFNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLTLAVMIQRFTFHL 489

BLAST of Lsi04G016470 vs. TAIR10
Match: AT3G14690.1 (AT3G14690.1 cytochrome P450, family 72, subfamily A, polypeptide 15)

HSP 1 Score: 337.4 bits (864), Expect = 1.4e-92
Identity = 186/489 (38.04%), Postives = 277/489 (56.65%), Query Frame = 1

Query: 24  WV---PWRIQIHFQKQGITGPSYRPIIGNATDIRRLYKEANAKPIPLTHDIVCRVLPYIH 83
           WV   P  ++ + ++QG+ G  Y P++G+      +  EA +KP+ LT DI  RV+PY  
Sbjct: 27  WVWFKPKMLEHYLRRQGLAGTPYTPLVGDLKKNFTMLSEARSKPLKLTDDISPRVVPYPL 86

Query: 84  RWSEEYGKMFVYWFGSKPRLAISDPEMIKEVLTNTGGPFRKVGFTPVSKLLFGEGLVGLE 143
           +  + YG+ +  WFG  P + I DPE IKEV  N    F+K    P++ ++  +GL   +
Sbjct: 87  QMFKTYGRTYFTWFGPIPTITIMDPEQIKEVF-NKVYDFQKPHTFPLATII-AKGLANYD 146

Query: 144 GEQWVLHRKIANQAFTIDRVKGWLPEITLSVRNVLEKWEEMKGGQ-EEFELDVHKQLRLL 203
           G++W  HR+I N AF I+++K  +P    S R V+ +W+++   +    E+DV   L  +
Sbjct: 147 GDKWAKHRRIINPAFHIEKIKNMVPAFHQSCREVVGEWDQLVSDKGSSCEVDVWPGLVSM 206

Query: 204 TADVISRTAFGSNFEEGKRIFNLQEQQMHHFLQAVSSVYIPGYRF--------------- 263
           TADVISRTAFGS+++EG+RIF LQ +     +QA    +IPGY +               
Sbjct: 207 TADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKAFIPGYSYLPTKSNRRMKAAARE 266

Query: 264 -------LPTKGNRERQRLEKETRASIKALVES--------------------------M 323
                  +  K  R R+  E  +   +  L+ES                           
Sbjct: 267 IQVILRGIVNKRLRAREAGEAPSDDLLGILLESNLRQTEGNGMSTEDLMEECKLFYFAGQ 326

Query: 324 ETTANLLTWALLLLAEHQEWQDRARKEVIKVCGHKTPPVADNLTELKLVGMIVNETLRLY 383
           ETT+ LL W ++LL++HQ+WQ RAR+EV +V G K P  A+ L +LK++ MI+ E LRLY
Sbjct: 327 ETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEPD-AEGLNQLKVMTMILYEVLRLY 386

Query: 384 PPAVMMMRRTTKRLTLGNVDVPEGTQLQLSVAAVHHDTDLWGEDASSFNPMRF----SEP 443
           PP   + R   K L LG++ +P G Q+ L +  V HD +LWG DA+ FNP RF    S+ 
Sbjct: 387 PPVTQLTRAIHKELKLGDLTLPGGVQISLPILLVQHDIELWGNDAAEFNPDRFKDGLSKA 446

Query: 444 RKHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQRFSFTVSPTYTHAPMMFVSLNPQF 457
            K   SF PF  GPRIC+GQ+ AL+EAK+A+A+I++RFSF +SP+Y HAP   ++++PQF
Sbjct: 447 TKSQVSFFPFAWGPRICIGQNFALLEAKMAMALILRRFSFEISPSYVHAPYTVITIHPQF 506

BLAST of Lsi04G016470 vs. TAIR10
Match: AT3G14620.1 (AT3G14620.1 cytochrome P450, family 72, subfamily A, polypeptide 8)

HSP 1 Score: 336.3 bits (861), Expect = 3.0e-92
Identity = 189/509 (37.13%), Postives = 287/509 (56.39%), Query Frame = 1

Query: 3   IIVPFVLLFVSILFKFLYANLWVPW----RIQIHFQKQGITGPSYRPIIGNATDIRRLYK 62
           + V   ++ V+ +  +++  L V W    + + + ++QG++G  +  ++G+      + +
Sbjct: 9   VAVAAAVVVVTTVTVWIWKGLNVAWLRPKKNEAYLKRQGLSGTPFTFLVGDIKREASMVE 68

Query: 63  EANAKPIPLTHDIVCRVLPYIHRWSEEYGKMFVYWFGSKPRLAISDPEMIKEVLTNTGGP 122
           +  ++PI LT D   RV+P I +  +++GK    W G    + ++ PE IK+VL N    
Sbjct: 69  QEKSRPINLTDDYTHRVMPLIQQTVKDHGKTSYMWMGPIASVIVTKPEHIKDVL-NRVYD 128

Query: 123 FRKVGFTPVSKLLFGEGLVGLEGEQWVLHRKIANQAFTIDRVKGWLPEITLSVRNVLEKW 182
           F K    P+ +L F  G+   EGE+W  HRKI N +F ++++K  +P    S   ++ KW
Sbjct: 129 FPKPPVHPIVEL-FATGVALYEGEKWSKHRKIINPSFHLEKLKIMIPAFYESCSEMISKW 188

Query: 183 EEMKGGQ-EEFELDVHKQLRLLTADVISRTAFGSNFEEGKRIFNLQEQQMHHFLQAVSSV 242
           E++   Q    E+DV   L  LT+DVISRTAFGS++EEGKRIF LQE+Q    L+A+   
Sbjct: 189 EKLVTEQGSSNEIDVWPYLGDLTSDVISRTAFGSSYEEGKRIFELQEEQGRRVLKALELA 248

Query: 243 YIPGYRFLPTKGNRERQRLEKETRASIKALV----------------------------- 302
           +IPG RFLPTK N   +++ KE ++ ++ ++                             
Sbjct: 249 FIPGMRFLPTKNNLRMRQINKEVKSRLREIIMKRQRGMDTGEAPKNDLLGILLESNSGDH 308

Query: 303 ----------------ESMETTANLLTWALLLLAEHQEWQDRARKEVIKVCGHKTPPVAD 362
                              ETTA LL W +++L+ HQ+WQD+AR+E++KV G    P  D
Sbjct: 309 GMSIEDVVEECRLFHFAGQETTAVLLVWTMIMLSHHQKWQDQAREEILKVIGKNNKPNFD 368

Query: 363 NLTELKLVGMIVNETLRLYPPAVMMMRRTTKRLTLG-NVDVPEGTQLQLSVAAVHHDTDL 422
            L+ LK + MI+NE LRLYPP +++ R   K   LG ++ +P G Q+ + V  VH D +L
Sbjct: 369 ALSRLKTMSMILNEVLRLYPPGILLGRTVEKETKLGEDMTLPGGAQVVIPVLMVHRDPEL 428

Query: 423 WGEDASSFNPMRF----SEPRKHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQRFSF 457
           WGED   FNP RF    S+  K+  SFLPFG GPR C GQ+ AL+EAK+AL +I+QRFSF
Sbjct: 429 WGEDVHEFNPERFADGISKATKNQVSFLPFGWGPRFCPGQNFALMEAKMALVLILQRFSF 488

BLAST of Lsi04G016470 vs. TAIR10
Match: AT1G17060.1 (AT1G17060.1 cytochrome p450 72c1)

HSP 1 Score: 330.1 bits (845), Expect = 2.2e-90
Identity = 170/470 (36.17%), Postives = 281/470 (59.79%), Query Frame = 1

Query: 7   FVLLFVSILFKFLYANL-WV---PWRIQIHFQKQGITGPSYRPIIGNATDIRRLYKEANA 66
           F++ F+ ++  +++  + WV   P R++ + +KQG +G SYR ++G+  +  ++ + A++
Sbjct: 11  FLIGFLILILNWVWRAVNWVWLRPKRLEKYLKKQGFSGNSYRILMGDMRESNQMDQVAHS 70

Query: 67  KPIPLTHDIVCRVLPYIHRWSEEYGKMFVYWFGSKPRLAISDPEMIKEVLTNTG-GPFRK 126
            P+PL  D + R++P++H    ++GK    W+G  P + + DPE ++E+++     P  K
Sbjct: 71  LPLPLDADFLPRMMPFLHHTVLKHGKKCFTWYGPYPNVIVMDPETLREIMSKHELFPKPK 130

Query: 127 VGFTPVSKLLFGEGLVGLEGEQWVLHRKIANQAFTIDRVKGWLPEITLSVRNVLEKWEEM 186
           +G       +F  GL+  EG +W  HR I N AF ID +K  LP    S + +LE+WE +
Sbjct: 131 IGS---HNHVFLSGLLNHEGPKWSKHRSILNPAFRIDNLKSILPAFNSSCKEMLEEWERL 190

Query: 187 KGGQEEFELDVHKQLRLLTADVISRTAFGSNFEEGKRIFNLQEQQMHHFLQAVSSVYIPG 246
              +   ELD       LT ++++R +FG ++++G +IF +Q++Q+   L A+ +VYIPG
Sbjct: 191 ASAKGTMELDSWTHCHDLTRNMLARASFGDSYKDGIKIFEIQQEQIDLGLLAIRAVYIPG 250

Query: 247 YRFLPTKGNRERQRLEKETRASIKALVESME-----------TTANLLTWALLLLAEHQE 306
            +FLPTK NR  +  E++ RA  KA++E+ E            T++L  W L+ L++HQ+
Sbjct: 251 SKFLPTKFNRRLRETERDMRAMFKAMIETKEEEIKRGRAGQNVTSSLFVWTLVALSQHQD 310

Query: 307 WQDRARKEVIKVCGHKTPPVADNLTELKLVGMIVNETLRLYPPAVMMMRRTTKRLTLGNV 366
           WQ++AR E+ +  G+  P   + L+ LK+V MI++E LRLY PA    R T + + L   
Sbjct: 311 WQNKARDEISQAFGNNEPDF-EGLSHLKVVTMILHEVLRLYSPAYFTCRITKQEVKLERF 370

Query: 367 DVPEGTQLQLSVAAVHHDTDLWGEDASSFNPMRFSE----PRKHLASFLPFGLGPRICVG 426
            +PEG  + + +  VHHD+DLWG+D   F P RF+       K   SFLPF  GPR C+G
Sbjct: 371 SLPEGVVVTIPMLLVHHDSDLWGDDVKEFKPERFANGVAGATKGRLSFLPFSSGPRTCIG 430

Query: 427 QHLALIEAKVALAMIIQRFSFTVSPTYTHAPMMFVSLNPQFGVQLLVRSL 457
           Q+ ++++AK+ LAM++QRFS  +SP+YTHAP    +  PQ G  L++R L
Sbjct: 431 QNFSMLQAKLFLAMVLQRFSVELSPSYTHAPFPAATTFPQHGAHLIIRKL 476

BLAST of Lsi04G016470 vs. TAIR10
Match: AT3G14680.1 (AT3G14680.1 cytochrome P450, family 72, subfamily A, polypeptide 14)

HSP 1 Score: 316.6 bits (810), Expect = 2.5e-86
Identity = 181/486 (37.24%), Postives = 269/486 (55.35%), Query Frame = 1

Query: 24  WVPWRIQIHFQKQGITGPSYRPIIGNATDIRRLYKEANAKPIPLTHDIVCRVLPYIHRWS 83
           + P  ++   ++QG++G SY P+IG+   +  ++ EA +KPI  T DI  RV+P+  +  
Sbjct: 30  FTPKMLERSLRRQGLSGTSYTPLIGDFKKMISMFIEATSKPIKPTDDITPRVMPHPLQML 89

Query: 84  EEYGKMFVYWFGSKPRLAISDPEMIKEVLTNTGGPFRKVGFTPVSKLLFGEGLVGLEGEQ 143
           + +G+  + WFG  P + I DPE IKEV  N    F+K    P+SK+L G GLV  +G++
Sbjct: 90  KTHGRTNLTWFGPIPTITIMDPEQIKEVF-NKVYDFQKAHTFPLSKIL-GTGLVSYDGDK 149

Query: 144 WVLHRKIANQAFTIDRVKGWLPEITLSVRNVLEKWEEMKGGQ-EEFELDVHKQLRLLTAD 203
           W  HR+I N AF ++++K  +     S   ++ +W+++   +    E+DV   L  +TAD
Sbjct: 150 WAQHRRIINPAFHLEKIKNMVHVFHESCSELVGEWDKLVSDKGSSCEVDVWPGLTSMTAD 209

Query: 204 VISRTAFGSNFEEGKRIFNLQEQQMHHFLQA----------------------VSSVYIP 263
           VISRTAFGS++ EG RIF LQ +     +QA                       +     
Sbjct: 210 VISRTAFGSSYREGHRIFELQAELAQLVMQAFQKFFIPGYIYLPTKGNRRMKTAAREIQD 269

Query: 264 GYRFLPTKGNRERQRLEKETRASIKALVES--------------------------METT 323
             R +  K  R R+  E  +   +  L+ES                           ETT
Sbjct: 270 ILRGIINKRERARESGEAPSEDLLGILLESNLGQTEGNGMSTEDMMEECKLFYLAGQETT 329

Query: 324 ANLLTWALLLLAEHQEWQDRARKEVIKVCGHKTPPVADNLTELKLVGMIVNETLRLYPPA 383
           + LL W ++LL++HQ+WQ RAR+EV +V G K P   + L +LK++ MI+ E LRLYPP 
Sbjct: 330 SVLLVWTMVLLSQHQDWQARAREEVKQVFGDKQPDT-EGLNQLKVMTMILYEVLRLYPPV 389

Query: 384 VMMMRRTTKRLTLGNVDVPEGTQLQLSVAAVHHDTDLWGEDASSFNPMRF----SEPRKH 443
           V + R   K + LG++ +P G Q+ L V  VH DT+LWG DA  F P RF    S+  K+
Sbjct: 390 VQLTRAIHKEMKLGDLTLPGGVQISLPVLLVHRDTELWGNDAGEFKPERFKDGLSKATKN 449

Query: 444 LASFLPFGLGPRICVGQHLALIEAKVALAMIIQRFSFTVSPTYTHAPMMFVSLNPQFGVQ 457
             SF PF  GPRIC+GQ+  L+EAK+A+++I+QRFSF +SP+Y HAP   ++L PQFG  
Sbjct: 450 QVSFFPFAWGPRICIGQNFTLLEAKMAMSLILQRFSFELSPSYVHAPYTIITLYPQFGAH 509

BLAST of Lsi04G016470 vs. NCBI nr
Match: gi|659133182|ref|XP_008466598.1| (PREDICTED: cytochrome P450 734A1-like [Cucumis melo])

HSP 1 Score: 709.5 bits (1830), Expect = 3.7e-201
Identity = 376/511 (73.58%), Postives = 405/511 (79.26%), Query Frame = 1

Query: 1   MYIIVPFVLLFVSI-LFKFLYANLWVPWRIQIHFQKQGITGPSYRPIIGNATDIRRLYKE 60
           M I++  VLLF +I +FKFLY+N WVPWRIQIHFQKQGITGPSYRPIIGNATDIRRLY E
Sbjct: 1   MSILLVSVLLFFTIFIFKFLYSNFWVPWRIQIHFQKQGITGPSYRPIIGNATDIRRLYIE 60

Query: 61  ANAKPIPLTHDIVCRVLPYIHRWSEEYGKMFVYWFGSKPRLAISDPEMIKEVLTNTGGPF 120
           A AKPIPLTHDIVCRVLPYIH+WS+EYGKMFVYWFG KPRL ISDP+MIKEVL NTGGPF
Sbjct: 61  AQAKPIPLTHDIVCRVLPYIHQWSKEYGKMFVYWFGPKPRLTISDPDMIKEVLMNTGGPF 120

Query: 121 RKVGFTPVSKLLFGEGLVGLEGEQWVLHRKIANQAFTIDRVKGWLPEITLSVRNVLEKWE 180
           RKVGFTPVSKLLFGEGLVGLE EQWV+HR+IANQAFTIDRVKGWLPEI  SVRNVLEKWE
Sbjct: 121 RKVGFTPVSKLLFGEGLVGLEDEQWVVHRRIANQAFTIDRVKGWLPEIATSVRNVLEKWE 180

Query: 181 EMKGGQEEFELDVHKQLRLLTADVISRTAFGSNFEEGKRIFNLQEQQMHHFLQAVSSVYI 240
           +M+GG EEFELDVHKQLR LTADVISRTAFGSNFEEGKRIFNLQEQQMHHFLQAVSSVYI
Sbjct: 181 KMRGGNEEFELDVHKQLRNLTADVISRTAFGSNFEEGKRIFNLQEQQMHHFLQAVSSVYI 240

Query: 241 PGYRFLPTKGNRERQRLEKETRASIKALVES------METTANLLTWALLLLAEHQEWQD 300
           PGYRFLPTK NRER+RLEKETRASIK LVES       E + NLL+   LLL+ ++    
Sbjct: 241 PGYRFLPTKTNRERERLEKETRASIKTLVESEENNKEREKSTNLLS---LLLSSYKGQNG 300

Query: 301 RARK----EVIKVC------GHKTPPVADNLTELKLV------------GMIVNETLR-- 360
              K    EV+  C      G +T   A+ LT   L+              ++N   +  
Sbjct: 301 EIEKLRVDEVVNECKTFYFAGMET--TANLLTWALLLLAEHQEWQDRAREEVINACGQKM 360

Query: 361 ----------------------LYPPAVMMMRRTTKRLTLGNVDVPEGTQLQLSVAAVHH 420
                                 LYPPAVMMMRRT K L LG VDVPEGTQLQLSV A+HH
Sbjct: 361 PPTTNNLTELKLVGMIINETLRLYPPAVMMMRRTMKHLRLGKVDVPEGTQLQLSVVAIHH 420

Query: 421 DTDLWGEDASSFNPMRFSEPRKHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQRFSF 459
           + +LWGEDASSFNPMRFSE RKH+ASFLPFGLGPRICVGQHLALIEAKVALAMIIQRFSF
Sbjct: 421 EKELWGEDASSFNPMRFSESRKHMASFLPFGLGPRICVGQHLALIEAKVALAMIIQRFSF 480

BLAST of Lsi04G016470 vs. NCBI nr
Match: gi|778688995|ref|XP_004147813.2| (PREDICTED: cytochrome P450 734A6-like [Cucumis sativus])

HSP 1 Score: 688.0 bits (1774), Expect = 1.2e-194
Identity = 368/511 (72.02%), Postives = 402/511 (78.67%), Query Frame = 1

Query: 1   MYIIVPFVLLFVSI-LFKFLYANLWVPWRIQIHFQKQGITGPSYRPIIGNATDIRRLYKE 60
           MYI+   VLLF++I +FKF+YAN WVPWRIQIHF+KQGITGP Y PIIGNATD+RR+Y E
Sbjct: 1   MYILAVSVLLFLTIFIFKFVYANFWVPWRIQIHFRKQGITGPRYCPIIGNATDMRRMYME 60

Query: 61  ANAKPIPLTHDIVCRVLPYIHRWSEEYGKMFVYWFGSKPRLAISDPEMIKEVLTNTGGPF 120
           A AK IPLTHDI+CRVLPYIH+WS EYGKMFVYWFG KPRLAISDP MIKEVLTNTGGPF
Sbjct: 61  AQAKTIPLTHDILCRVLPYIHQWSTEYGKMFVYWFGPKPRLAISDPVMIKEVLTNTGGPF 120

Query: 121 RKVGFTPVSKLLFGEGLVGLEGEQWVLHRKIANQAFTIDRVKGWLPEITLSVRNVLEKWE 180
           RKVGFTPVSKLLFGEGLVGLE +QWV+HR+IANQAFTIDRVKGWLPEITLSVRNVLEKWE
Sbjct: 121 RKVGFTPVSKLLFGEGLVGLEDKQWVIHRRIANQAFTIDRVKGWLPEITLSVRNVLEKWE 180

Query: 181 EMKGGQEEFELDVHKQLRLLTADVISRTAFGSNFEEGKRIFNLQEQQMHHFLQAVSSVYI 240
           EMK G EEFE+DVHKQLRLLTADVISRTAFGSNFEEGKRIFNLQEQQM+HFLQAVSSVYI
Sbjct: 181 EMKEGMEEFEVDVHKQLRLLTADVISRTAFGSNFEEGKRIFNLQEQQMNHFLQAVSSVYI 240

Query: 241 PGYRFLPTKGNRERQRLEKETRASIKALVES------METTANLLTWALLLLAEHQEWQD 300
           PGYRFLPTK NRER RLEKETRASIKALVES       E ++NLL+   LLL+ ++    
Sbjct: 241 PGYRFLPTKMNRERDRLEKETRASIKALVESEKNNKEKENSSNLLS---LLLSSYKNQNG 300

Query: 301 RARK----EVIKVC------GHKTPPVADNLTELKLV------------GMIVNETLRLY 360
              K    EV+  C      G +T   A+ LT   L+              ++N   +  
Sbjct: 301 EIEKLGVDEVVNECKTFYFAGMET--TANLLTWALLLLAEHQEWQDRAREEVINVCGQKT 360

Query: 361 PPA---------VMMMRRTTKRLT---------------LGNVDVPEGTQLQLSVAAVHH 420
           PP          V M+   T RL                LGN+DVPEGTQLQLSV A+HH
Sbjct: 361 PPTSDNLTELKLVGMIINETLRLYPPATMMMRRTMKRLTLGNIDVPEGTQLQLSVVAIHH 420

Query: 421 DTDLWGEDASSFNPMRFSEPRKHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQRFSF 459
           D +LWGEDAS+FNPMRFSEPRKHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQ+FSF
Sbjct: 421 DKELWGEDASNFNPMRFSEPRKHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQQFSF 480

BLAST of Lsi04G016470 vs. NCBI nr
Match: gi|659133180|ref|XP_008466596.1| (PREDICTED: cytochrome P450 734A6-like [Cucumis melo])

HSP 1 Score: 679.9 bits (1753), Expect = 3.2e-192
Identity = 365/509 (71.71%), Postives = 400/509 (78.59%), Query Frame = 1

Query: 1   MYIIVPFVLLFVSI-LFKFLYANLWVPWRIQIHFQKQGITGPSYRPIIGNATDIRRLYKE 60
           MYI+   VLLF++I +FKFLY+N WVPWRIQIHFQKQGITGP YRPIIGNATD+RR+Y E
Sbjct: 1   MYILGVSVLLFLTIFIFKFLYSNFWVPWRIQIHFQKQGITGPRYRPIIGNATDMRRMYME 60

Query: 61  ANAKPIPLTHDIVCRVLPYIHRWSEEYGKMFVYWFGSKPRLAISDPEMIKEVLTNTGGPF 120
           A AK IPLTHDIVCRVLPYIH+WS EYGKMFVYWFG KPRLAISDP MIKEVL NTGGPF
Sbjct: 61  AQAKTIPLTHDIVCRVLPYIHQWSMEYGKMFVYWFGPKPRLAISDPVMIKEVLANTGGPF 120

Query: 121 RKVGFTPVSKLLFGEGLVGLEGEQWVLHRKIANQAFTIDRVKGWLPEITLSVRNVLEKWE 180
           RKVGFTPVSKLLFGEGLVGLE EQWV+HR+IANQAFTIDRVKGWLPEITLSVRNVL+KWE
Sbjct: 121 RKVGFTPVSKLLFGEGLVGLEDEQWVVHRRIANQAFTIDRVKGWLPEITLSVRNVLDKWE 180

Query: 181 EMKGGQEEFELDVHKQLRLLTADVISRTAFGSNFEEGKRIFNLQEQQMHHFLQAVSSVYI 240
           EMKGG EEFE+DVHKQLRLLTADVISRTAFGSNFEEGKRIFNLQEQQM+HFLQAVSSVYI
Sbjct: 181 EMKGGVEEFEVDVHKQLRLLTADVISRTAFGSNFEEGKRIFNLQEQQMNHFLQAVSSVYI 240

Query: 241 PGYRFLPTKGNRERQRLEKETRASIKALVES------METTANLLTWALLLLAEHQ---- 300
           PGYRFLPTK NRER RLEKETRASIKALVES       E + NLL+   LLL+ ++    
Sbjct: 241 PGYRFLPTKMNRERDRLEKETRASIKALVESEKNRKERENSTNLLS---LLLSSYKNQNG 300

Query: 301 EWQDRARKEVIKVC------GHKTPPVADNLTELKLV------------GMIVNETLRLY 360
           E +     EV+  C      G +T   A+ LT   L+              IVN   +  
Sbjct: 301 EIETLGVDEVVNECKTFYFAGMET--TANLLTWALLLLAEHQEWQDRAREEIVNVCGQKT 360

Query: 361 PPAV----------MMMRRTTKR--------------LTLGNVDVPEGTQLQLSVAAVHH 420
           PP            M++  T +               LTLG++D+PEGTQLQLSV A+HH
Sbjct: 361 PPTADNLTELKLVGMIVNETLRLYPPAIMMMRRTMKRLTLGSIDIPEGTQLQLSVVAIHH 420

Query: 421 DTDLWGEDASSFNPMRFSEPRKHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQRFSF 457
           DT+LWGEDA +FNP RFSE RKHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQRF+F
Sbjct: 421 DTELWGEDAHNFNPKRFSESRKHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQRFAF 480

BLAST of Lsi04G016470 vs. NCBI nr
Match: gi|778688998|ref|XP_004147812.2| (PREDICTED: cytochrome P450 734A1-like [Cucumis sativus])

HSP 1 Score: 588.2 bits (1515), Expect = 1.3e-164
Identity = 317/452 (70.13%), Postives = 347/452 (76.77%), Query Frame = 1

Query: 59  EANAKPIPLTHDIVCRVLPYIHRWSEEYGKMFVYWFGSKPRLAISDPEMIKEVLTNTGGP 118
           EA AK IPLTHDI+CRVLPYIH+WS EYGKMFVYWFG KPRL ISDP MIKE+LTNTGGP
Sbjct: 2   EAQAKTIPLTHDIICRVLPYIHQWSMEYGKMFVYWFGPKPRLTISDPVMIKEILTNTGGP 61

Query: 119 FRKVGFTPVSKLLFGEGLVGLEGEQWVLHRKIANQAFTIDRVKGWLPEITLSVRNVLEKW 178
           FRKVGFTPVSKLLFGEGLVGLE EQWV+HR+IANQAFTIDRVKGWLPEITLSVRNVL+KW
Sbjct: 62  FRKVGFTPVSKLLFGEGLVGLEDEQWVVHRRIANQAFTIDRVKGWLPEITLSVRNVLDKW 121

Query: 179 EEMKGGQEEFELDVHKQLRLLTADVISRTAFGSNFEEGKRIFNLQEQQMHHFLQAVSSVY 238
           EEMK G EEFE+DVHKQLRLLTADVISRTAFGSNFEEGKRIFNLQEQQM+HFLQAVSSVY
Sbjct: 122 EEMKEGMEEFEVDVHKQLRLLTADVISRTAFGSNFEEGKRIFNLQEQQMNHFLQAVSSVY 181

Query: 239 IPGYRFLPTKGNRERQRLEKETRASIKALVES------METTANLLTWALLLLAEHQ--- 298
           IPGYRFLPTK NRER RLEKETRASIKALVES       E + NLL+   LLL+ ++   
Sbjct: 182 IPGYRFLPTKMNRERDRLEKETRASIKALVESEKNRKERENSTNLLS---LLLSSYKNQN 241

Query: 299 -EWQDRARKEVIKVC------GHKTPPVADNLTELKLV------------GMIVNETLRL 358
            E ++    EV+  C      G +T   A+ LT   L+              ++N   + 
Sbjct: 242 GEIENLEVDEVVNECKTFYFAGMET--TANLLTWALLLLAEHQEWQDRAREEVINVCGQK 301

Query: 359 YPPA---------VMMMRRTTKRLTLGNV---------------DVPEGTQLQLSVAAVH 418
            PP          V M+   T RL    +               DVPEGTQLQLSV A+H
Sbjct: 302 TPPTADNLTELKLVGMIVNETLRLYPPAIMMMRRTMKRLTLGNIDVPEGTQLQLSVVAIH 361

Query: 419 HDTDLWGEDASSFNPMRFSEPRKHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQRFS 459
           HD +LWGEDA +FNPMRFSEPRKHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQRF+
Sbjct: 362 HDKELWGEDAHNFNPMRFSEPRKHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQRFA 421

BLAST of Lsi04G016470 vs. NCBI nr
Match: gi|902180096|gb|KNA09474.1| (hypothetical protein SOVF_153300 [Spinacia oleracea])

HSP 1 Score: 548.1 bits (1411), Expect = 1.4e-152
Identity = 267/513 (52.05%), Postives = 348/513 (67.84%), Query Frame = 1

Query: 3   IIVPFVLLFVS--ILFKFLYANLWVPWRIQIHFQKQGITGPSYRPIIGNATDIRRLYKEA 62
           +++PF++L +   IL K  +  LWVPW IQ HF KQGI GP+YRPI GN   IR+LY EA
Sbjct: 5   LLLPFLVLIICTLILLKLTHTLLWVPWSIQRHFNKQGIVGPTYRPIFGNTGQIRQLYSEA 64

Query: 63  NAK------------PIPLTHDIVCRVLPYIHRWSEEYGKMFVYWFGSKPRLAISDPEMI 122
            +K            P+ + HDI  RV+P+ H+WS  YG  F+YWFG KP LAISDP MI
Sbjct: 65  QSKTIQIFPGGPGEDPLVINHDITHRVMPFYHKWSAIYGNTFLYWFGPKPMLAISDPLMI 124

Query: 123 KEVLTNTGGPFRKVGFTPVSKLLFGEGLVGLEGEQWVLHRKIANQAFTIDRVKGWLPEIT 182
           KEVL N    F+K+ + P++K+LFG+GLVGL+G+ W  HR+IANQAF+++RVK W+P+I 
Sbjct: 125 KEVLINNNVLFKKLKYNPLAKMLFGQGLVGLDGDHWAFHRRIANQAFSMERVKSWIPDIV 184

Query: 183 LSVRNVLEKWEEMKGGQEEFELDVHKQLRLLTADVISRTAFGSNFEEGKRIFNLQEQQMH 242
            S R  L +WEE KG QEEFE++VHK+L  L+AD+ISRTAFGS++EEGKRIF LQEQQMH
Sbjct: 185 ASTRKTLIEWEETKGSQEEFEMEVHKELHNLSADIISRTAFGSSYEEGKRIFELQEQQMH 244

Query: 243 HFLQAVSSVYIPGYRFLPTKGNRERQRLEKETRASIKALVES------------------ 302
               A+ S+YIPG+RFLPT  N+ER RL+KETR SIK L+                    
Sbjct: 245 LVSLALRSIYIPGFRFLPTAKNKERWRLDKETRDSIKLLMTKNSETTRNKSNLLSLFMSA 304

Query: 303 ---------------------------METTANLLTWALLLLAEHQEWQDRARKEVIKVC 362
                                       ETTANLLTWAL++L+ HQEWQ++AR EV +VC
Sbjct: 305 TENEGGKVKRLSIDEVVEECKTFYFAGKETTANLLTWALIMLSAHQEWQNKARDEVFQVC 364

Query: 363 GHKTPPVADNLTELKLVGMIVNETLRLYPPAVMMMRRTTKRLTLGNVDVPEGTQLQLSVA 422
           G    P  DN+ + KL+ MIVNETLRLYPPAVMM RR  K + LGN+ +P  T L L++ 
Sbjct: 365 GVHGLPTVDNINDFKLIKMIVNETLRLYPPAVMMKRRAGKEVKLGNLRIPAETLLYLAMT 424

Query: 423 AVHHDTDLWGEDASSFNPMRFSEPRKHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQ 457
            VHHD ++WGEDA+ FNP+RF EPRKH A+F PF LGPR+CVGQ+LA+IE+ + LA+I++
Sbjct: 425 NVHHDVNVWGEDANKFNPLRFLEPRKHPATFFPFSLGPRVCVGQNLAMIESIIVLAIILR 484

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C734A_ARATH1.8e-9436.74Cytochrome P450 734A1 OS=Arabidopsis thaliana GN=CYP734A1 PE=2 SV=1[more]
C7345_ORYSJ1.4e-9137.40Cytochrome P450 734A5 OS=Oryza sativa subsp. japonica GN=CYP734A5 PE=2 SV=1[more]
C7A15_ARATH2.4e-9138.04Cytochrome P450 72A15 OS=Arabidopsis thaliana GN=CYP72A15 PE=2 SV=1[more]
C7A14_ARATH4.4e-8537.24Cytochrome P450 72A14 OS=Arabidopsis thaliana GN=CYP72A14 PE=2 SV=1[more]
C7A13_ARATH2.9e-8436.27Cytochrome P450 72A13 OS=Arabidopsis thaliana GN=CYP72A13 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0K9QQC9_SPIOL1.0e-15252.05Uncharacterized protein OS=Spinacia oleracea GN=SOVF_153300 PE=3 SV=1[more]
A0A072U2N1_MEDTR2.5e-15153.56Cytochrome P450 family 709 protein OS=Medicago truncatula GN=MTR_7g098550 PE=3 S... [more]
A0A061FBM4_THECC2.7e-15053.09Cytochrome P450, family 721, subfamily A, polypeptide 1, putative OS=Theobroma c... [more]
K7LDC7_SOYBN2.5e-14853.47Uncharacterized protein OS=Glycine max GN=GLYMA_09G117400 PE=3 SV=1[more]
A0A0L9UTG7_PHAAN2.5e-14852.68Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan06g133900 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G26710.11.0e-9536.74 Cytochrome P450 superfamily protein[more]
AT3G14690.11.4e-9238.04 cytochrome P450, family 72, subfamily A, polypeptide 15[more]
AT3G14620.13.0e-9237.13 cytochrome P450, family 72, subfamily A, polypeptide 8[more]
AT1G17060.12.2e-9036.17 cytochrome p450 72c1[more]
AT3G14680.12.5e-8637.24 cytochrome P450, family 72, subfamily A, polypeptide 14[more]
Match NameE-valueIdentityDescription
gi|659133182|ref|XP_008466598.1|3.7e-20173.58PREDICTED: cytochrome P450 734A1-like [Cucumis melo][more]
gi|778688995|ref|XP_004147813.2|1.2e-19472.02PREDICTED: cytochrome P450 734A6-like [Cucumis sativus][more]
gi|659133180|ref|XP_008466596.1|3.2e-19271.71PREDICTED: cytochrome P450 734A6-like [Cucumis melo][more]
gi|778688998|ref|XP_004147812.2|1.3e-16470.13PREDICTED: cytochrome P450 734A1-like [Cucumis sativus][more]
gi|902180096|gb|KNA09474.1|1.4e-15252.05hypothetical protein SOVF_153300 [Spinacia oleracea][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0020037heme binding
GO:0005506iron ion binding
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Vocabulary: INTERPRO
TermDefinition
IPR017972Cyt_P450_CS
IPR002401Cyt_P450_E_grp-I
IPR001128Cyt_P450
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G016470.1Lsi04G016470.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 395..404
score: 1.0E-13coord: 322..333
score: 1.0E-13coord: 404..415
score: 1.0E-13coord: 269..286
score: 1.0
IPR001128Cytochrome P450GENE3DG3DSA:1.10.630.10coord: 269..455
score: 1.5E-63coord: 20..268
score: 7.9
IPR001128Cytochrome P450PFAMPF00067p450coord: 264..437
score: 5.6E-52coord: 79..269
score: 4.5
IPR001128Cytochrome P450unknownSSF48264Cytochrome P450coord: 36..454
score: 1.7
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 107..128
score: 2.7E-17coord: 394..404
score: 2.7E-17coord: 404..427
score: 2.7E-17coord: 83..102
score: 2.7E-17coord: 199..217
score: 2.7
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 397..406
scor
NoneNo IPR availablePANTHERPTHR24282CYTOCHROME P450 FAMILY MEMBERcoord: 1..458
score: 5.9E
NoneNo IPR availablePANTHERPTHR24282:SF45CYTOCHROME P450, FAMILY 721, SUBFAMILY A, POLYPEPTIDE 1coord: 1..458
score: 5.9E

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Lsi04G016470Cla97C05G100620Watermelon (97103) v2lsiwmbB288
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi04G016470Wild cucumber (PI 183967)cpilsiB115
Lsi04G016470Wild cucumber (PI 183967)cpilsiB190
Lsi04G016470Cucumber (Chinese Long) v2culsiB118
Lsi04G016470Cucumber (Chinese Long) v2culsiB192
Lsi04G016470Melon (DHL92) v3.5.1lsimeB302
Lsi04G016470Melon (DHL92) v3.5.1lsimeB311
Lsi04G016470Watermelon (Charleston Gray)lsiwcgB287
Lsi04G016470Watermelon (Charleston Gray)lsiwcgB307
Lsi04G016470Watermelon (97103) v1lsiwmB295
Lsi04G016470Watermelon (97103) v1lsiwmB316
Lsi04G016470Cucumber (Gy14) v2cgyblsiB106
Lsi04G016470Cucumber (Gy14) v2cgyblsiB176
Lsi04G016470Melon (DHL92) v3.6.1lsimedB316
Lsi04G016470Melon (DHL92) v3.6.1lsimedB325
Lsi04G016470Silver-seed gourdcarlsiB094
Lsi04G016470Silver-seed gourdcarlsiB163
Lsi04G016470Silver-seed gourdcarlsiB863
Lsi04G016470Silver-seed gourdcarlsiB894
Lsi04G016470Cucumber (Chinese Long) v3cuclsiB130
Lsi04G016470Cucumber (Chinese Long) v3cuclsiB209
Lsi04G016470Watermelon (97103) v2lsiwmbB307
Lsi04G016470Wax gourdlsiwgoB346
Lsi04G016470Bottle gourd (USVL1VR-Ls)lsilsiB150
Lsi04G016470Cucumber (Gy14) v1cgylsiB377
Lsi04G016470Cucumber (Gy14) v1cgylsiB552
Lsi04G016470Cucurbita maxima (Rimu)cmalsiB024
Lsi04G016470Cucurbita maxima (Rimu)cmalsiB175
Lsi04G016470Cucurbita maxima (Rimu)cmalsiB424
Lsi04G016470Cucurbita maxima (Rimu)cmalsiB725
Lsi04G016470Cucurbita moschata (Rifu)cmolsiB012
Lsi04G016470Cucurbita moschata (Rifu)cmolsiB165
Lsi04G016470Cucurbita moschata (Rifu)cmolsiB412
Lsi04G016470Cucurbita moschata (Rifu)cmolsiB717
Lsi04G016470Cucurbita pepo (Zucchini)cpelsiB086
Lsi04G016470Cucurbita pepo (Zucchini)cpelsiB462
Lsi04G016470Cucurbita pepo (Zucchini)cpelsiB638
Lsi04G016470Cucurbita pepo (Zucchini)cpelsiB674