Lsi04G013560 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi04G013560
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionPheophorbide a oxygenase, chloroplastic
Locationchr04 : 21297238 .. 21300352 (-)
   



The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTGGTTCAGAGAACTGCCTTACTCTTATAACCAAAGAAACCTTCACCTTCAACTTAATCCAATGTTCACCTTCCCTCCAAAACCCTAATTCCTTTTTCCCTCTCTCAATTCAACCTCTCCCATGGCTTCGCTCCGCCCATTTCTTTCCACTGCCCCTTTCTCCTACGCGCCTAAGCCCACGAGCCTCACGAATTCTCTTCTTCTTCACGCTCCATCCTCTTCCTCGTTCAAATTAGTCGCCGGCAAAAGGAGATTCGCGGTGCGCACCCGCGCCGTCGCAGGCGCAGAAACTGCAGCTCAGATCGGCGATCTGAAGGACGATGATGGTCAGGTGGTTCTTTTCGGTCCGGCGATCGACGAGGAGCCTGGCGGTGAAGCTACGGCGGAGTACGATTGGACTGAAGAGTGGTACCCGTTGTACCTGACGCGGAATGTGCCGGAGGATGCTCCGTTGGGGCTCACCGTGTTCGATAAGCAGTTGGTTTTGTACCGCGATGGAACAGGCGAGCTCCGCTGTCACGAAGATAGGTGCCCACACAGGTAATCTTAAAATTTTCCCAAAAAGAGAGAAATAAAACATTGGCCCATGCAGGTTTCGAACCTGCGACCTTCGCGTTATTAGCACGACGCTCTAACCAACTGAGCTAATAGGCCAGTTGAAAATCTGATCGCTATTCAGTTTTAGTCTATTTTTGGTTTCAATTTTTTATTTCCGATTTTCTCTCTGTTATATTATCTCTGTTTTTTTCATTCCTCATACGCATCTTCAATTACAATTTTTTTTCAATAAATGAAGCGATTATTCAAGGGAACATAAGAATTCTAATTAAAGGTAAATTACAATTTACTTCTTAAGCTTTGACCTCTTGTATCTATTTGGTTTTTGTTCTTTTAATAATGTCTAATAAATGTACGTACTTATAATTTTGTGTCTAATAAATCTATAAATTTTGTTATTTTGATAGTTCGAGACCAAATAGACATGAATTACGGATTAAATATTTCATTAAACCTTCAGATTATAACTTAGAATGAATCTTCTTAACTTTCTTCTACTTACATAGTCTGGTTTTAGGCTTGCAAAGCTCTCTGAAGGCCAATTAATCGATGGCAATCTGGAATGTCTTTACCATGGCTGGCAATTTGAAGGCGAGGGGAAATGTGTGAAGATTCCTCAGGTAATAGAGCCTATGAGTTCGTTTAGATTAATTTTCTAAATGTTTAAAAATAAATATTTTTTCTTCTCAGCTAAAAACATTTTTTAAACATTTAAAAAGTCATTTCAAACAAGAGCTATATAGCTTGTATATTTCGGTGCAATATAAAACTCGGAGGGATATATGGAAGAATTATGTATTTGGTCAACCTAACTCTAACAACTTCAATAGTGGCACTACTAGAGTTGCATAAAGACAAATTTCCTAACTCCAGACTTCATAACTCAACTTAGTCTTCAAATACTCGAGCTATTTTGGTATAAATTTTAAGGAGGGTGAAAAATACTCTTAGAAAATCTCAATTACTTTCTCTTTAGCCAATTATTTGTCTACATAGTTACAACATTTAAAAAACACTTCAATAGACTTTAAATCATTTTTGTTTAGAGAAGTCCCTTTAGTTAGTACAAGTGTTTATAGGAAGTATTTTCGATTAAAAGTATTATTTCCAAAAATCATTCAAAATATATGTTTACTCTTTTATGTGAAGTAGTTTTTATATAATTACAACTTTTTATAAAAAAAAAATATATATTAATTGCCTTAATCCATCTTCAACTTAGCTGAGAACTCTGCAATACAGCTTCCATCAGATGCCAAGATTCCTAGATCCGCATGTCTAAGAACATACGAGGTAAGAGACTCACAGGGAGTTGTATGGGTGTGGATGTCTTTGGAAAAGCCTCCAACCCTAAAAAAGATACCACGGTTCGAGAACTTCGACCGGCCCGGGTTTCAGGACATTTCGAGTGTTCACGAGCTTCCTTATGATCACTCCATACTTCTTGAGAACCTTATGGATCCTGCACATATACCTATCTCCCACGATAGAACAGATTTTACAGCCAGAAGGGAAGATGCTCAGCCACTTGGATTCGAGATTACCGAGCGTACAAATCGCGGTTTCGCCGGTCGGTGGGGCCGTGCTAAAGATCAACCATTGCCAAACTTCTTAAGGTTTGAAGCACCTTGCGTCCTTCAGAATAACAGGGAAATGCCCAATGATAAAGGTGGGATGGATTACTTTACAGGACTTTTCCTTTGTAGACCTACAGGCCAGGGGAAATCCATGCTTATAGTGAGATTTGGTAGCACAAGATCATCTCTCTTGGTAAAATTGCTGCCTACATGGTTTATGCACCAAAATGCCTGCAAAGTTTTTGAGCAAGATATGGGATTTCTTTCATCCCAAAATGAGGTTCTAATGAGAGAAAAAGTACCTACAAAGGAACTCTATCTCAACTTACGATCGTCCGATACTTTTGTTCTCGAGTACCGACGATGGATGGACAAGGTTGGGAACGGGATGCCGTATTATTTTGGACACAGTACAATTTCGTTGCCGAAAGTGCCTGCCGTTGTCGAACACGCCCCGGTGGGACTAGTTGCTGGAGCTTCAGCTTCTCCTCCGGCTAAGGGGGGAATCGGAACTATGTATGCTCCAAACTTGTCGAATCGATACTTTCGACATATAATTCATTGCAGAAGTTGTAGCAATACCATCAAATCTTTTCAAACTTGGAACAAGGCTTTTTCTGCCATTGGCATCACATTGGTTGCATTGGCAATTTTGGTGAGTGGAAGGCAATGGAAGGCAGTCATTCTGTTGTCAGCAGCATTATGCTTTGGTGGAGCTTATGCATGCTCAGCTATTGTGGCTTTAAATACAACAAATTTCATAAGAACTCACAGAAGGTTGTAAGTTGTTTATAGATTTCAAAATATTAGAGTATTGATAGTGTATATACTTTGTAAATTTCTGCTGTTTGATGATTTCCTAAGAACAGACAACAGAGTGATTGAACATATGGAAATTCTTTCCAGTTTAGTGTCTTCTTGCTAGCTTTGCTTGAATGTGAAACACTATGGATCTCGCTCGATAACAATTTGGTTTCTG

mRNA sequence

TGTGGTTCAGAGAACTGCCTTACTCTTATAACCAAAGAAACCTTCACCTTCAACTTAATCCAATGTTCACCTTCCCTCCAAAACCCTAATTCCTTTTTCCCTCTCTCAATTCAACCTCTCCCATGGCTTCGCTCCGCCCATTTCTTTCCACTGCCCCTTTCTCCTACGCGCCTAAGCCCACGAGCCTCACGAATTCTCTTCTTCTTCACGCTCCATCCTCTTCCTCGTTCAAATTAGTCGCCGGCAAAAGGAGATTCGCGGTGCGCACCCGCGCCGTCGCAGGCGCAGAAACTGCAGCTCAGATCGGCGATCTGAAGGACGATGATGGTCAGGTGGTTCTTTTCGGTCCGGCGATCGACGAGGAGCCTGGCGGTGAAGCTACGGCGGAGTACGATTGGACTGAAGAGTGGTACCCGTTGTACCTGACGCGGAATGTGCCGGAGGATGCTCCGTTGGGGCTCACCGTGTTCGATAAGCAGTTGGTTTTGTACCGCGATGGAACAGGCGAGCTCCGCTGTCACGAAGATAGGTGCCCACACAGAGAAGTCCCTTTAGTTAGTACAAGTGTTTATAGGAAGTATTTTCGATTAAAACTTCCATCAGATGCCAAGATTCCTAGATCCGCATGTCTAAGAACATACGAGGTAAGAGACTCACAGGGAGTTGTATGGGTGTGGATGTCTTTGGAAAAGCCTCCAACCCTAAAAAAGATACCACGGTTCGAGAACTTCGACCGGCCCGGGTTTCAGGACATTTCGAGTGTTCACGAGCTTCCTTATGATCACTCCATACTTCTTGAGAACCTTATGGATCCTGCACATATACCTATCTCCCACGATAGAACAGATTTTACAGCCAGAAGGGAAGATGCTCAGCCACTTGGATTCGAGATTACCGAGCGTACAAATCGCGGTTTCGCCGGTCGGTGGGGCCGTGCTAAAGATCAACCATTGCCAAACTTCTTAAGGTTTGAAGCACCTTGCGTCCTTCAGAATAACAGGGAAATGCCCAATGATAAAGGTGGGATGGATTACTTTACAGGACTTTTCCTTTGTAGACCTACAGGCCAGGGGAAATCCATGCTTATAGTGAGATTTGGTAGCACAAGATCATCTCTCTTGGTAAAATTGCTGCCTACATGGTTTATGCACCAAAATGCCTGCAAAGTTTTTGAGCAAGATATGGGATTTCTTTCATCCCAAAATGAGGTTCTAATGAGAGAAAAAGTACCTACAAAGGAACTCTATCTCAACTTACGATCGTCCGATACTTTTGTTCTCGAGTACCGACGATGGATGGACAAGGTTGGGAACGGGATGCCGTATTATTTTGGACACAGTACAATTTCGTTGCCGAAAGTGCCTGCCGTTGTCGAACACGCCCCGGTGGGACTAGTTGCTGGAGCTTCAGCTTCTCCTCCGGCTAAGGGGGGAATCGGAACTATGTATGCTCCAAACTTGTCGAATCGATACTTTCGACATATAATTCATTGCAGAAGTTGTAGCAATACCATCAAATCTTTTCAAACTTGGAACAAGGCTTTTTCTGCCATTGGCATCACATTGGTTGCATTGGCAATTTTGGTGAGTGGAAGGCAATGGAAGGCAGTCATTCTGTTGTCAGCAGCATTATGCTTTGGTGGAGCTTATGCATGCTCAGCTATTGTGGCTTTAAATACAACAAATTTCATAAGAACTCACAGAAGGTTGTAAGTTGTTTATAGATTTCAAAATATTAGAGTATTGATAGTGTATATACTTTGTAAATTTCTGCTGTTTGATGATTTCCTAAGAACAGACAACAGAGTGATTGAACATATGGAAATTCTTTCCAGTTTAGTGTCTTCTTGCTAGCTTTGCTTGAATGTGAAACACTATGGATCTCGCTCGATAACAATTTGGTTTCTG

Coding sequence (CDS)

ATGGCTTCGCTCCGCCCATTTCTTTCCACTGCCCCTTTCTCCTACGCGCCTAAGCCCACGAGCCTCACGAATTCTCTTCTTCTTCACGCTCCATCCTCTTCCTCGTTCAAATTAGTCGCCGGCAAAAGGAGATTCGCGGTGCGCACCCGCGCCGTCGCAGGCGCAGAAACTGCAGCTCAGATCGGCGATCTGAAGGACGATGATGGTCAGGTGGTTCTTTTCGGTCCGGCGATCGACGAGGAGCCTGGCGGTGAAGCTACGGCGGAGTACGATTGGACTGAAGAGTGGTACCCGTTGTACCTGACGCGGAATGTGCCGGAGGATGCTCCGTTGGGGCTCACCGTGTTCGATAAGCAGTTGGTTTTGTACCGCGATGGAACAGGCGAGCTCCGCTGTCACGAAGATAGGTGCCCACACAGAGAAGTCCCTTTAGTTAGTACAAGTGTTTATAGGAAGTATTTTCGATTAAAACTTCCATCAGATGCCAAGATTCCTAGATCCGCATGTCTAAGAACATACGAGGTAAGAGACTCACAGGGAGTTGTATGGGTGTGGATGTCTTTGGAAAAGCCTCCAACCCTAAAAAAGATACCACGGTTCGAGAACTTCGACCGGCCCGGGTTTCAGGACATTTCGAGTGTTCACGAGCTTCCTTATGATCACTCCATACTTCTTGAGAACCTTATGGATCCTGCACATATACCTATCTCCCACGATAGAACAGATTTTACAGCCAGAAGGGAAGATGCTCAGCCACTTGGATTCGAGATTACCGAGCGTACAAATCGCGGTTTCGCCGGTCGGTGGGGCCGTGCTAAAGATCAACCATTGCCAAACTTCTTAAGGTTTGAAGCACCTTGCGTCCTTCAGAATAACAGGGAAATGCCCAATGATAAAGGTGGGATGGATTACTTTACAGGACTTTTCCTTTGTAGACCTACAGGCCAGGGGAAATCCATGCTTATAGTGAGATTTGGTAGCACAAGATCATCTCTCTTGGTAAAATTGCTGCCTACATGGTTTATGCACCAAAATGCCTGCAAAGTTTTTGAGCAAGATATGGGATTTCTTTCATCCCAAAATGAGGTTCTAATGAGAGAAAAAGTACCTACAAAGGAACTCTATCTCAACTTACGATCGTCCGATACTTTTGTTCTCGAGTACCGACGATGGATGGACAAGGTTGGGAACGGGATGCCGTATTATTTTGGACACAGTACAATTTCGTTGCCGAAAGTGCCTGCCGTTGTCGAACACGCCCCGGTGGGACTAGTTGCTGGAGCTTCAGCTTCTCCTCCGGCTAAGGGGGGAATCGGAACTATGTATGCTCCAAACTTGTCGAATCGATACTTTCGACATATAATTCATTGCAGAAGTTGTAGCAATACCATCAAATCTTTTCAAACTTGGAACAAGGCTTTTTCTGCCATTGGCATCACATTGGTTGCATTGGCAATTTTGGTGAGTGGAAGGCAATGGAAGGCAGTCATTCTGTTGTCAGCAGCATTATGCTTTGGTGGAGCTTATGCATGCTCAGCTATTGTGGCTTTAAATACAACAAATTTCATAAGAACTCACAGAAGGTTGTAA

Protein sequence

MASLRPFLSTAPFSYAPKPTSLTNSLLLHAPSSSSFKLVAGKRRFAVRTRAVAGAETAAQIGDLKDDDGQVVLFGPAIDEEPGGEATAEYDWTEEWYPLYLTRNVPEDAPLGLTVFDKQLVLYRDGTGELRCHEDRCPHREVPLVSTSVYRKYFRLKLPSDAKIPRSACLRTYEVRDSQGVVWVWMSLEKPPTLKKIPRFENFDRPGFQDISSVHELPYDHSILLENLMDPAHIPISHDRTDFTARREDAQPLGFEITERTNRGFAGRWGRAKDQPLPNFLRFEAPCVLQNNREMPNDKGGMDYFTGLFLCRPTGQGKSMLIVRFGSTRSSLLVKLLPTWFMHQNACKVFEQDMGFLSSQNEVLMREKVPTKELYLNLRSSDTFVLEYRRWMDKVGNGMPYYFGHSTISLPKVPAVVEHAPVGLVAGASASPPAKGGIGTMYAPNLSNRYFRHIIHCRSCSNTIKSFQTWNKAFSAIGITLVALAILVSGRQWKAVILLSAALCFGGAYACSAIVALNTTNFIRTHRRL
BLAST of Lsi04G013560 vs. Swiss-Prot
Match: TIC55_PEA (Protein TIC 55, chloroplastic OS=Pisum sativum GN=TIC55 PE=1 SV=1)

HSP 1 Score: 701.0 bits (1808), Expect = 9.6e-201
Identity = 348/550 (63.27%), Postives = 416/550 (75.64%), Query Frame = 1

Query: 1   MASLRPFL--STAPFSYAPKPTSLTNSLLLHAPSSS-SFKLVAGKRRFAVRTRAVAGAET 60
           +AS   FL  +   F+    P     +LL   PSS+ SF      RR   +   VA A  
Sbjct: 5   LASANSFLLPTKTHFALHVSPPPSKKTLLCTNPSSNFSFNKALSSRR-RKQAWCVAAAAD 64

Query: 61  AAQIGDLKDDDGQVVLFGPAIDEEPGGEA-TAEYDWTEEWYPLYLTRNVPEDAPLGLTVF 120
                 L  ++ Q VL GP+ ++E  GE   A+YDWTEEWYPLYLT+NVP DAPLGL V+
Sbjct: 65  VKDATLLDGEEDQKVLVGPSSEQERKGEREVADYDWTEEWYPLYLTKNVPHDAPLGLKVY 124

Query: 121 DKQLVLYRDGTGELRCHEDRCPHR-----EVPLVSTSVYRKYFRL------------KLP 180
           DK +VL+RDG  + +C+EDRCPHR     E  L+   +   Y               +LP
Sbjct: 125 DKNIVLFRDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLP 184

Query: 181 SDAKIPRSACLRTYEVRDSQGVVWVWMSLEKPPTLKKIPRFENFDRPGFQDISSVHELPY 240
           +DAKIP+SAC++TYEVRDSQGV+WVWMS + PP + KIP FENF RPGFQDIS+ HELPY
Sbjct: 185 ADAKIPKSACVKTYEVRDSQGVLWVWMSRKTPPNVSKIPWFENFARPGFQDISTTHELPY 244

Query: 241 DHSILLENLMDPAHIPISHDRTDFTARREDAQPLGFEITERTNRGFAGRWGRAKDQPLPN 300
           DHSILLENLMDPAH+PISHDRTD++A+REDAQ LGFE+TERT+RGFAG WGR KD   PN
Sbjct: 245 DHSILLENLMDPAHVPISHDRTDWSAKREDAQALGFEVTERTDRGFAGWWGREKDGSKPN 304

Query: 301 FLRFEAPCVLQNNREMPNDKGGMDYFTGLFLCRPTGQGKSMLIVRFGSTRSSLLVKLLPT 360
           FLRFEAPCVLQNNRE+ +  G +++F+GLFLCRPTGQGKSMLIVRFG+T+ S L+KL P 
Sbjct: 305 FLRFEAPCVLQNNREIVDKNGEINHFSGLFLCRPTGQGKSMLIVRFGATKRSPLIKLFPE 364

Query: 361 WFMHQNACKVFEQDMGFLSSQNEVLMREKVPTKELYLNLRSSDTFVLEYRRWMDKVGNGM 420
           W+ HQNA KVFEQDMGFLSSQNE+L++EKVPTKELYLNL+SSDT+V EYR+WMDKVG+GM
Sbjct: 365 WYFHQNASKVFEQDMGFLSSQNEILLKEKVPTKELYLNLKSSDTWVAEYRKWMDKVGHGM 424

Query: 421 PYYFGHSTISLPKVPAVVEHAPVGLVAGASASPPAKGGIGTMYAPNLSNRYFRHIIHCRS 480
           PY+FGHSTISLP+ PAVVEHAP GLVAG SAS PAKGGIGTM+APNL+NRYFRH+IHC+ 
Sbjct: 425 PYHFGHSTISLPEEPAVVEHAPAGLVAGLSASSPAKGGIGTMHAPNLANRYFRHVIHCKG 484

Query: 481 CSNTIKSFQTWNKAFSAIGITLVALAILVSGRQWKAVILLSAALCFGGAYACSAIVALNT 530
           CS+ IK+FQ W    S + + L ALAILVSGRQWK ++L SA+LC  G YACS  +A+NT
Sbjct: 485 CSSAIKAFQIWKNVLSGVVVALAALAILVSGRQWKVLLLASASLCSVGVYACSTAIAMNT 544

BLAST of Lsi04G013560 vs. Swiss-Prot
Match: TIC55_ARATH (Protein TIC 55, chloroplastic OS=Arabidopsis thaliana GN=TIC55 PE=1 SV=1)

HSP 1 Score: 680.2 bits (1754), Expect = 1.7e-194
Identity = 348/545 (63.85%), Postives = 403/545 (73.94%), Query Frame = 1

Query: 6   PFLSTAPFSYAPKP---TSLTNSLLLHAPSSSSFKLVAGKRRFAVRTRAVAGAETAAQIG 65
           PFLS++       P   T +T + ++H   S+   +    R   +R  AVAG   + Q  
Sbjct: 4   PFLSSSLQLTPTSPILFTKVTPTPIIHNHRSTC-TIPTKPRLRLLRRSAVAGTAVSDQT- 63

Query: 66  DLKDDDGQVVLFGPAIDEEPGGEATAEYDWTEEWYPLYLTRNVPEDAPLGLTVFDKQLVL 125
               + G  VL  P   EE      A+YDWTEEWYPLYLT+NVPEDAPLGLTV+D+Q+VL
Sbjct: 64  ----EGGGDVLLNP---EEEKRVEVADYDWTEEWYPLYLTKNVPEDAPLGLTVYDRQIVL 123

Query: 126 YRDGTGELRCHEDRCPHR-----EVPLVSTSVYRKYFRLK------------LPSDAKIP 185
           Y+DG G LRC+EDRCPHR     E  L+   +   Y   +            LP+ AKIP
Sbjct: 124 YKDGEGTLRCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPASAKIP 183

Query: 186 RSACLRTYEVRDSQGVVWVWMSLEKPPTLKKIPRFENFDRPGFQDISSVHELPYDHSILL 245
           ++AC++TYEV+DSQGVVWVWMS + PP  +K+P FENF RPGF DIS+ HELPYDHSILL
Sbjct: 184 KAACVKTYEVKDSQGVVWVWMSTKTPPNPEKLPWFENFARPGFFDISTTHELPYDHSILL 243

Query: 246 ENLMDPAHIPISHDRTDFTARREDAQPLGFEITERTNRGFAGRWGRAKDQPL-PNFLRFE 305
           ENLMDPAH+PISHDRTDFTA+REDAQPL FE+TER+NRGFAG WGR K+     N LRF+
Sbjct: 244 ENLMDPAHVPISHDRTDFTAKREDAQPLVFEVTERSNRGFAGTWGREKEGGKGSNLLRFD 303

Query: 306 APCVLQNNREMPNDKGGMDYFTGLFLCRPTGQGKSMLIVRFGSTRSSLLVKLLPTWFMHQ 365
           APCVLQNNRE     G  +YF+GLFLCRPTGQGKSMLIVRFG T+ S LV +LP WF HQ
Sbjct: 304 APCVLQNNREFEGKDGVKNYFSGLFLCRPTGQGKSMLIVRFGVTKRSPLVSVLPQWFWHQ 363

Query: 366 NACKVFEQDMGFLSSQNEVLMREKVPTKELYLNLRSSDTFVLEYRRWMDKVGNGMPYYFG 425
           NACKVFEQDMGFLSSQNEVLM+EKVPTK+LYLNL+SSDT+V EYR+WMDKVG+GMPY+FG
Sbjct: 364 NACKVFEQDMGFLSSQNEVLMKEKVPTKDLYLNLKSSDTWVAEYRKWMDKVGHGMPYHFG 423

Query: 426 HSTISLPKVPAVVEHAPVGLVAGASASPPAKGGIGTMYAPNLSNRYFRHIIHCRSCSNTI 485
           H TISLPKVP VVEHAP GL+A  SAS PAKGGIGTM+APNL+NRYFRHIIHCRSCSN I
Sbjct: 424 HRTISLPKVPPVVEHAPAGLIAALSASYPAKGGIGTMHAPNLANRYFRHIIHCRSCSNVI 483

Query: 486 KSFQTWNKAFSAIGITLVALAILVSGRQWKAVILLSAALCFGGAYACSAIVALNTTNFIR 530
           KSF+ W    SA  + L ALAILV  RQWKAV+L SAALC   AY C   + LNT NFIR
Sbjct: 484 KSFELWKNILSATAVALTALAILVVSRQWKAVLLGSAALCSAAAYTCLRAINLNTNNFIR 539

BLAST of Lsi04G013560 vs. Swiss-Prot
Match: CAO_CHLRE (Chlorophyllide a oxygenase, chloroplastic OS=Chlamydomonas reinhardtii GN=CAO PE=2 SV=2)

HSP 1 Score: 92.8 bits (229), Expect = 1.2e-17
Identity = 77/331 (23.26%), Postives = 130/331 (39.27%), Query Frame = 1

Query: 96  WYPLYLTRNVPEDAPLGLTVFDKQLVLYRDGTGELRCHEDRCPHREVPL-----VSTSVY 155
           WYP   +  +P+D  +   +F +  V++RD  G+  C  D C HR  PL     V   V 
Sbjct: 305 WYPAEFSARLPKDTLVPFELFGEPWVMFRDEKGQPSCIRDECAHRGCPLSLGKVVEGQVM 364

Query: 156 RKYFRLKLPSD---AKIPRSACLRTYEV-----RDSQGVVWVWMSLEKPPTLKKIPRFEN 215
             Y   +   D    K+P +   R   V      +  G +WVW     P   + +P F  
Sbjct: 365 CPYHGWEFNGDGACTKMPSTPFCRNVGVAALPCAEKDGFIWVWPGDGLP--AETLPDFAQ 424

Query: 216 FDRPGFQDISSVHELPYDHSILLENLMDPAHIPISHDRTDFTARREDAQPLGFEITERTN 275
                      + ++P +H +L+ENL+D AH P +H     T+      P+   +    N
Sbjct: 425 PPEGFLIHAEIMVDVPVEHGLLIENLLDLAHAPFTH-----TSTFARGWPVPDFVKFHAN 484

Query: 276 RGFAGRWGRAKDQPLPNFLRFEAPCVLQNNREMPNDKGGM---------DYFTGLFLCRP 335
           +  +G W      P P  + F+ PC+  +   +      M         ++   L +C P
Sbjct: 485 KALSGFW-----DPYPIDMAFQPPCMTLSTIGLAQPGKIMRGVTASQCKNHLHQLHVCMP 544

Query: 336 TGQGKSMLIVRFGSTRSSLLVKLLPTWFMH---------QNACKVFEQDMGFLSSQNEVL 395
           + +G + L+ R       + +  LP W  H         Q A +V  +D+  +  Q + +
Sbjct: 545 SKKGHTRLLYR-------MSLDFLP-WMRHVPFIDRIWKQVAAQVLGEDLVLVLGQQDRM 604

BLAST of Lsi04G013560 vs. Swiss-Prot
Match: PTC52_ARATH (Protochlorophyllide-dependent translocon component 52, chloroplastic OS=Arabidopsis thaliana GN=PTC52 PE=2 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 2.0e-17
Identity = 105/503 (20.87%), Postives = 177/503 (35.19%), Query Frame = 1

Query: 81  EPGGEATAEYDWTEEWYPLYLTRNVPEDAPLGLTVFDKQLVLYRD-GTGELRCHEDRCPH 140
           EPG +   ++DW   WYP+    ++ +  P G  V    LV++ D    + +  +D CPH
Sbjct: 73  EPGSD---KFDWYANWYPVMPICDLDKKVPHGKKVMGIDLVVWWDRNEKQWKVMDDTCPH 132

Query: 141 --------REVPLVSTSVYRKYFRLKLPSDAK-IPRS------------ACLRTYEVRDS 200
                   R             +      D K IP++            AC+  Y     
Sbjct: 133 RLAPLSDGRIDQWGRLQCVYHGWCFNGSGDCKLIPQAPPDGPPVHTFKQACVAVYPSTVQ 192

Query: 201 QGVVWVWMSLEKPPTLKKI------PRFENFDRPGFQDISSVHELPYDHSILLENLMDPA 260
             ++W W + +  P  K I      P     + P F  +    ++PY + +L+ENLMDPA
Sbjct: 193 HEIIWFWPNSD--PKYKNIIETNKPPYIPELEDPSFTKLMGNRDIPYGYDVLVENLMDPA 252

Query: 261 HIPISHDRTDFTARREDAQPLGFEITERTNRGF----------AGRWGRAKDQPLP---- 320
           H+P +H            +P G  I   +N  F          A +  R   +PL     
Sbjct: 253 HVPYAH-----YGLMRFPKPKGKYIICISNSCFNPFTNLQILLAEKIDREGGKPLEINVK 312

Query: 321 -------------NFLRFEAPCVLQNN--------REMP----NDKGGMD--YFTGLFLC 380
                         +  F APCV +++         E P    +DK  +     + +F+C
Sbjct: 313 KLDNKGFFSKQEWGYSNFIAPCVYRSSTDPLPEQEHEYPAPAASDKAALSKRRLSLIFIC 372

Query: 381 RPTGQGKSMLIVRFGSTRSSLLVKLLPTWFMHQNACKVFEQDMGFLS-SQNEVLMREKVP 440
            P   G+S LI  F       + K++P W  H     + + D+  L   + ++L R    
Sbjct: 373 IPVSPGRSRLIWTFPRNFGVFIDKIVPRWVFHIGQNTILDSDLHLLHVEERKILERGPEN 432

Query: 441 TKELYLNLRSSDTFVLEYRRWMDKVGNGMPYYFG-HSTISLPKVPAVVEHAPVGLVAGAS 500
            ++       SD  V+ +RRW +K       + G      LP  P               
Sbjct: 433 WQKACFIPTKSDANVVTFRRWFNKYSEARVDWRGKFDPFLLPPTPP-------------- 492

Query: 501 ASPPAKGGIGTMYAPNLSNRYFRHIIHCRSCSNTIKSFQTWNKAFSAIGITLVALAILVS 509
                           L +RY+ H+ +C SC    K             + ++ +  ++ 
Sbjct: 493 -------------REQLFDRYWSHVENCSSCKKAHKYLNALEVILQIASVAMIGVMAVLK 538

BLAST of Lsi04G013560 vs. Swiss-Prot
Match: TSAM1_COMTE (Toluene-4-sulfonate monooxygenase system iron-sulfur subunit TsaM1 OS=Comamonas testosteroni GN=tsaM1 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.8e-10
Identity = 45/188 (23.94%), Postives = 76/188 (40.43%), Query Frame = 1

Query: 96  WYPLYLTRNVPEDAPLGLTVFDKQLVLYRDGTGELRCHEDRCPHREVPL-----VSTSVY 155
           WY       +P +     T+ ++ ++LYRD  G +   E+RC HR  PL         V 
Sbjct: 7   WYVAAWDTEIPAEGLFHRTLLNEPVLLYRDTQGRVVALENRCCHRSAPLHIGRQEGDCVR 66

Query: 156 RKYFRLK---------LPSDAKIPRSACLRTYEVRDSQGVVWVWMSLEKPPTLKKIPRFE 215
             Y  LK         +P   +IP   C+++Y V +   +VW+WM          I  + 
Sbjct: 67  CLYHGLKFNPSGACVEIPGQEQIPPKTCIKSYPVVERNRLVWIWMGDPARANPDDIVDYF 126

Query: 216 NFDRPGFQDISSVHELPYDHSILLENLMDPAHIPISHDRTDFTARREDAQPLGFEITERT 270
             D P ++          ++ ++++NL+D  H+   H  T  T      +P+    T  T
Sbjct: 127 WHDSPEWRMKPGYIHYQANYKLIVDNLLDFTHLAWVHPTTLGTDSAASLKPVIERDTTGT 186

BLAST of Lsi04G013560 vs. TrEMBL
Match: A0A0A0L0A9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G651770 PE=4 SV=1)

HSP 1 Score: 864.4 bits (2232), Expect = 7.3e-248
Identity = 429/547 (78.43%), Postives = 464/547 (84.83%), Query Frame = 1

Query: 1   MASLRPFLSTAPFSYAPKPTSLTNSLLLHAPS-SSSFKLVAGKRRFAVRTRAVAGAETAA 60
           MASLRPFLS +P S AP PT  TN  LLH+P  SSSFKLVA K RFAVRT AVAGA+ A 
Sbjct: 1   MASLRPFLSLSPVSPAPNPTRPTNFHLLHSPLFSSSFKLVATKTRFAVRTAAVAGAQIAD 60

Query: 61  QIGDLKDDDGQVVLFGPAIDEEPGGEATAEYDWTEEWYPLYLTRNVPEDAPLGLTVFDKQ 120
            +     DD QVVL GP   E+P  EATAEY+WTEEWYPLYLT++VPEDAPLGLTVFDKQ
Sbjct: 61  PV-----DDNQVVLLGPTTSEKPDDEATAEYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQ 120

Query: 121 LVLYRDGTGELRCHEDRCPHR-----EVPLVSTSVYRKYFRL------------KLPSDA 180
           LVLYRDG GEL CHEDRCPHR     E  L+   +   Y               +LPSDA
Sbjct: 121 LVLYRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPSDA 180

Query: 181 KIPRSACLRTYEVRDSQGVVWVWMSLEKPPTLKKIPRFENFDRPGFQDISSVHELPYDHS 240
           KIPRSACLRTYEVRDSQGVVWVWMSLEKP TLK IP FENFDRPGF+D SS+HELPYDHS
Sbjct: 181 KIPRSACLRTYEVRDSQGVVWVWMSLEKPSTLKSIPWFENFDRPGFRDFSSIHELPYDHS 240

Query: 241 ILLENLMDPAHIPISHDRTDFTARREDAQPLGFEITERTNRGFAGRWGRAKDQPLPNFLR 300
           ILLENLMDPAHIPISHDRT + A+REDAQPL FE+TERTNRGFAGRWG AKDQPL NFLR
Sbjct: 241 ILLENLMDPAHIPISHDRTSYPAKREDAQPLDFEVTERTNRGFAGRWGNAKDQPLSNFLR 300

Query: 301 FEAPCVLQNNREMPNDKGGMDYFTGLFLCRPTGQGKSMLIVRFGSTRSSLLVKLLPTWFM 360
           FEAPCVLQNNRE PN KGG+DYFTGLFLCRPTGQGKSM+IVRFGST S++ + L P WF+
Sbjct: 301 FEAPCVLQNNRETPNKKGGIDYFTGLFLCRPTGQGKSMVIVRFGSTASTVRLNLFPAWFL 360

Query: 361 HQNACKVFEQDMGFLSSQNEVLMREKVPTKELYLNLRSSDTFVLEYRRWMDKVGNGMPYY 420
           HQNAC+VFEQDMGFLSSQNEVLM+EKVPTKELYLNLRSSDT+VLEYRRWMDKVG+GMPYY
Sbjct: 361 HQNACRVFEQDMGFLSSQNEVLMKEKVPTKELYLNLRSSDTWVLEYRRWMDKVGHGMPYY 420

Query: 421 FGHSTISLPKVPAVVEHAPVGLVAGASASPPAKGGIGTMYAPNLSNRYFRHIIHCRSCSN 480
           FGHSTISLPK+PAV+EHAP G+VAG +ASPPAKGGIGTM+APNLSNRYFRHIIHCRSCSN
Sbjct: 421 FGHSTISLPKLPAVIEHAPAGIVAGTAASPPAKGGIGTMHAPNLSNRYFRHIIHCRSCSN 480

Query: 481 TIKSFQTWNKAFSAIGITLVALAILVSGRQWKAVILLSAALCFGGAYACSAIVALNTTNF 530
            IKSFQTWNKA SAI ITL+ALAILV+GRQWKA+ LLSAAL FGGAYACSA +ALNT NF
Sbjct: 481 VIKSFQTWNKALSAIAITLIALAILVNGRQWKALSLLSAALFFGGAYACSAALALNTDNF 540

BLAST of Lsi04G013560 vs. TrEMBL
Match: V4UBR3_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10007965mg PE=4 SV=1)

HSP 1 Score: 723.0 bits (1865), Expect = 2.6e-205
Identity = 352/518 (67.95%), Postives = 408/518 (78.76%), Query Frame = 1

Query: 31  PSSSSFKLVAGKRRFAVRTRAVAGAETAAQIGDLKDDDGQVVLFGPAIDEEPGGEA-TAE 90
           P +++  +   K   + +  AV    + + +GD K      VL GPA  EE  GE   A+
Sbjct: 23  PPTTNVIISLNKTPKSRKCHAVTDRSSTSTVGDHK------VLVGPASAEERRGERQVAD 82

Query: 91  YDWTEEWYPLYLTRNVPEDAPLGLTVFDKQLVLYRDGTGELRCHEDRCPHR-----EVPL 150
           YDWTEEWYPLYLT++VP+DAPLGLTVFD+Q+VLY+DG GELRC++DRCPHR     E  L
Sbjct: 83  YDWTEEWYPLYLTKDVPDDAPLGLTVFDQQIVLYKDGNGELRCYQDRCPHRLAKLSEGQL 142

Query: 151 VSTSVYRKYFRLK------------LPSDAKIPRSACLRTYEVRDSQGVVWVWMSLEKPP 210
           +   +   Y   +            LP+DAKIPRSAC+RTYEV++SQGVVWVWMS + PP
Sbjct: 143 IDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPRSACVRTYEVKESQGVVWVWMSQKTPP 202

Query: 211 TLKKIPRFENFDRPGFQDISSVHELPYDHSILLENLMDPAHIPISHDRTDFTARREDAQP 270
              K+P FENF RPGFQD+S++HELPYDHSILLENLMDPAHIPISHDRTD+TA+REDAQP
Sbjct: 203 NPDKLPWFENFARPGFQDVSTIHELPYDHSILLENLMDPAHIPISHDRTDWTAKREDAQP 262

Query: 271 LGFEITERTNRGFAGRWGRAKDQPLPNFLRFEAPCVLQNNREMPNDKGG-MDYFTGLFLC 330
           LGFE+TERT+RGFAGRWG+ KD+PLPNFLRFEAPCVLQNNRE+ + K G   YFTGLFLC
Sbjct: 263 LGFEVTERTDRGFAGRWGKEKDEPLPNFLRFEAPCVLQNNRELVDSKTGEKRYFTGLFLC 322

Query: 331 RPTGQGKSMLIVRFGSTRSSLLVKLLPTWFMHQNACKVFEQDMGFLSSQNEVLMREKVPT 390
           RPTGQGKSMLIVRFG+T+ S L KL P W+ HQNA KVFEQDMGFLSSQNEVLM+EKVPT
Sbjct: 323 RPTGQGKSMLIVRFGATKRSPLAKLFPKWYFHQNASKVFEQDMGFLSSQNEVLMKEKVPT 382

Query: 391 KELYLNLRSSDTFVLEYRRWMDKVGNGMPYYFGHSTISLPKVPAVVEHAPVGLVAGASAS 450
           KELYLNLRSSDT+V EYR+WMDKVG+GMPY+FGHSTISLPKVPAVVEHAP GLVAG SAS
Sbjct: 383 KELYLNLRSSDTWVAEYRKWMDKVGHGMPYHFGHSTISLPKVPAVVEHAPAGLVAGVSAS 442

Query: 451 PPAKGGIGTMYAPNLSNRYFRHIIHCRSCSNTIKSFQTWNKAFSAIGITLVALAILVSGR 510
            PAKG IGTM+APNL+NRYFRH+IHC+ CS+ IK+F TW  + S +   L  LAIL SGR
Sbjct: 443 SPAKGAIGTMHAPNLANRYFRHVIHCKGCSSVIKAFSTWKNSLSVVAAALTVLAILASGR 502

Query: 511 QWKAVILLSAALCFGGAYACSAIVALNTTNFIRTHRRL 530
           QWKA  L SA+LC  G YACS  +A NTTNFIRTHRRL
Sbjct: 503 QWKAFCLASASLCLAGVYACSTAIATNTTNFIRTHRRL 534

BLAST of Lsi04G013560 vs. TrEMBL
Match: G7J9S7_MEDTR (Translocon at the inner envelope membrane ofs protein 55-II OS=Medicago truncatula GN=MTR_3g105820 PE=4 SV=1)

HSP 1 Score: 722.6 bits (1864), Expect = 3.4e-205
Identity = 361/554 (65.16%), Postives = 428/554 (77.26%), Query Frame = 1

Query: 1   MASLRPFL--STAPFSYAPKPTSLTNSLLLHAPSSS-SFKLVAG----KRRFAVRTRAVA 60
           MA + PFL  +   F+    P      LL  +PSSS SF L  G    +R+ A    A A
Sbjct: 1   MALVNPFLLPTKTHFALPVSPPPSKQILLWTSPSSSFSFNLNKGLSSSRRKHAGYLPATA 60

Query: 61  GAETAAQIGDLKDDDGQVVLFGPAIDEEPGGEAT-AEYDWTEEWYPLYLTRNVPEDAPLG 120
             +TA+ + D+++D  Q VL GP+ ++E  GE   A YDW EEWYPLYLT+NVP+DAPLG
Sbjct: 61  DVKTASTLLDVEED--QKVLVGPSSEQERRGERELAAYDWKEEWYPLYLTKNVPDDAPLG 120

Query: 121 LTVFDKQLVLYRDGTGELRCHEDRCPHR-----EVPLVSTSVYRKYFRL----------- 180
           LTVFDK LVL++DG  + +C+EDRCPHR     E  L+   +   Y              
Sbjct: 121 LTVFDKNLVLFKDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKI 180

Query: 181 -KLPSDAKIPRSACLRTYEVRDSQGVVWVWMSLEKPPTLKKIPRFENFDRPGFQDISSVH 240
            +LP+DAKIPRSAC++TYEVRDSQGV+WVWMS + PP + KIP FENF RPGFQD+S+ H
Sbjct: 181 PQLPADAKIPRSACVKTYEVRDSQGVIWVWMSPKVPPNVSKIPWFENFARPGFQDVSTTH 240

Query: 241 ELPYDHSILLENLMDPAHIPISHDRTDFTARREDAQPLGFEITERTNRGFAGRWGRAKDQ 300
           ELPYDHSILLENLMDPAHIPISHDRTD+TA+REDAQPL FE+TERT+RGFAG WGR KD 
Sbjct: 241 ELPYDHSILLENLMDPAHIPISHDRTDWTAKREDAQPLLFEVTERTDRGFAGWWGREKDG 300

Query: 301 PLPNFLRFEAPCVLQNNREMPNDKGGMDYFTGLFLCRPTGQGKSMLIVRFGSTRSSLLVK 360
             PNFLRFEAPCVLQNNRE+ +  G ++YF+GLFLCRPTGQGKSMLIVRFG T+ S +VK
Sbjct: 301 TKPNFLRFEAPCVLQNNREIVDKNGEINYFSGLFLCRPTGQGKSMLIVRFGGTKRSPIVK 360

Query: 361 LLPTWFMHQNACKVFEQDMGFLSSQNEVLMREKVPTKELYLNLRSSDTFVLEYRRWMDKV 420
           L P W+ HQNA KVFEQDMGFLSSQNE+L++EKVPTKELYLNL+SSDT+V EYR+WMDKV
Sbjct: 361 LFPQWYFHQNASKVFEQDMGFLSSQNEILLKEKVPTKELYLNLKSSDTWVAEYRKWMDKV 420

Query: 421 GNGMPYYFGHSTISLPKVPAVVEHAPVGLVAGASASPPAKGGIGTMYAPNLSNRYFRHII 480
           G+GMPY+FGH+TISLPK PAV+EHAP GL+AG SAS PAKGGIGTM+APNL+NRYFRH+I
Sbjct: 421 GHGMPYHFGHNTISLPKEPAVIEHAPAGLIAGLSASSPAKGGIGTMHAPNLANRYFRHVI 480

Query: 481 HCRSCSNTIKSFQTWNKAFSAIGITLVALAILVSGRQWKAVILLSAALCFGGAYACSAIV 530
           HC+ C   +K+FQTW  A SA+ + L ALAILV+GRQWKA++L SAALC  G YACS  V
Sbjct: 481 HCKGCRTAVKAFQTWKNALSAVVVALAALAILVTGRQWKAILLASAALCSVGVYACSTAV 540

BLAST of Lsi04G013560 vs. TrEMBL
Match: A0A067FW11_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g039074mg PE=4 SV=1)

HSP 1 Score: 722.2 bits (1863), Expect = 4.5e-205
Identity = 351/518 (67.76%), Postives = 408/518 (78.76%), Query Frame = 1

Query: 31  PSSSSFKLVAGKRRFAVRTRAVAGAETAAQIGDLKDDDGQVVLFGPAIDEEPGGEA-TAE 90
           P +++  +   K   + +  AV    + + +GD K      VL GPA  EE  GE   A+
Sbjct: 23  PPTTNVIISLNKTPKSRKCHAVTDRSSTSTVGDHK------VLVGPASAEERRGERQVAD 82

Query: 91  YDWTEEWYPLYLTRNVPEDAPLGLTVFDKQLVLYRDGTGELRCHEDRCPHR-----EVPL 150
           YDWTEEWYPLYLT++VP+DAPLGLTVFD+Q+VLY+DG GELRC++DRCPHR     E  L
Sbjct: 83  YDWTEEWYPLYLTKDVPDDAPLGLTVFDQQIVLYKDGNGELRCYQDRCPHRLAKLSEGQL 142

Query: 151 VSTSVYRKYFRLK------------LPSDAKIPRSACLRTYEVRDSQGVVWVWMSLEKPP 210
           +   +   Y   +            LP+DAKIPRSAC+RTYEV++SQGVVWVWMS + PP
Sbjct: 143 IDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPRSACVRTYEVKESQGVVWVWMSQKTPP 202

Query: 211 TLKKIPRFENFDRPGFQDISSVHELPYDHSILLENLMDPAHIPISHDRTDFTARREDAQP 270
              K+P FENF RPGFQD+S++HELPYDHSILLENLMDPAHIPISHDRTD+TA+REDAQP
Sbjct: 203 NPDKLPWFENFARPGFQDVSTIHELPYDHSILLENLMDPAHIPISHDRTDWTAKREDAQP 262

Query: 271 LGFEITERTNRGFAGRWGRAKDQPLPNFLRFEAPCVLQNNREMPNDKGG-MDYFTGLFLC 330
           LGFE+TERT+RGFAGRWG+ KD+PLPNFLRFEAPCVLQNNRE+ + K G   YFTGLFLC
Sbjct: 263 LGFEVTERTDRGFAGRWGKEKDEPLPNFLRFEAPCVLQNNRELVDSKTGEKHYFTGLFLC 322

Query: 331 RPTGQGKSMLIVRFGSTRSSLLVKLLPTWFMHQNACKVFEQDMGFLSSQNEVLMREKVPT 390
           RPTGQGKSMLIVRFG+T+ S L KL P W+ HQNA KVFEQDMGFLSSQNEVLM+E VPT
Sbjct: 323 RPTGQGKSMLIVRFGATKRSPLAKLFPKWYFHQNASKVFEQDMGFLSSQNEVLMKETVPT 382

Query: 391 KELYLNLRSSDTFVLEYRRWMDKVGNGMPYYFGHSTISLPKVPAVVEHAPVGLVAGASAS 450
           KELYLNLRSSDT+V EYR+WMDKVG+GMPY+FGHSTISLPKVPAVVEHAP GLVAG SAS
Sbjct: 383 KELYLNLRSSDTWVAEYRKWMDKVGHGMPYHFGHSTISLPKVPAVVEHAPAGLVAGVSAS 442

Query: 451 PPAKGGIGTMYAPNLSNRYFRHIIHCRSCSNTIKSFQTWNKAFSAIGITLVALAILVSGR 510
            PAKG IGTM+APNL+NRYFRH+IHC+ CS+ IK+F TW  + S +   L  LAIL SGR
Sbjct: 443 SPAKGAIGTMHAPNLANRYFRHVIHCKGCSSVIKAFSTWKNSLSVVAAALTVLAILASGR 502

Query: 511 QWKAVILLSAALCFGGAYACSAIVALNTTNFIRTHRRL 530
           QWKA  L SA+LC  G YACS  +A+NTTNFIRTHRRL
Sbjct: 503 QWKAFCLASASLCLAGVYACSTAIAMNTTNFIRTHRRL 534

BLAST of Lsi04G013560 vs. TrEMBL
Match: B9IL92_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0018s02890g PE=4 SV=1)

HSP 1 Score: 719.9 bits (1857), Expect = 2.2e-204
Identity = 346/542 (63.84%), Postives = 418/542 (77.12%), Query Frame = 1

Query: 6   PFLSTAPFSYAPKPTSLTNSLLLHAPSSSSFKLVAGKRRFAVRTRAVAGAETAAQIGDLK 65
           PF S  P +  P    + + LL         K +  K  +  +  AVA A TA       
Sbjct: 17  PFASFTPTTKLPTTIHINSHLL---------KSIHSKPYYIKKCHAVADAPTAIPPSLDA 76

Query: 66  DDDGQVVLFGPAIDEEPGGE-ATAEYDWTEEWYPLYLTRNVPEDAPLGLTVFDKQLVLYR 125
           +DD   VL GP+ +EE  G+ A  +Y+WTEEWYPLYLT++VP+DAPLGL VFDKQLVLY+
Sbjct: 77  EDDAHGVLIGPSSEEERRGDGAVVDYNWTEEWYPLYLTKDVPDDAPLGLNVFDKQLVLYK 136

Query: 126 DGTGELRCHEDRCPHR-----EVPLVSTSVYRKYFRL------------KLPSDAKIPRS 185
           DG GELRCHEDRCPHR     E  L+   +   Y               +LP++AKIP+S
Sbjct: 137 DGQGELRCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPANAKIPQS 196

Query: 186 ACLRTYEVRDSQGVVWVWMSLEKPPTLKKIPRFENFDRPGFQDISSVHELPYDHSILLEN 245
           AC++TYEV++SQGVVWVWMSL+ PP  KK+P FENF RPGFQD S++HELPYDHSILLEN
Sbjct: 197 ACVKTYEVKESQGVVWVWMSLKTPPNFKKVPWFENFARPGFQDTSTIHELPYDHSILLEN 256

Query: 246 LMDPAHIPISHDRTDFTARREDAQPLGFEITERTNRGFAGRWGRAKDQPLPNFLRFEAPC 305
           LMDPAHIPISHDRTD+T++REDAQ L FE+TERT+RGFAGRWG+ KDQ L +FLRF+APC
Sbjct: 257 LMDPAHIPISHDRTDWTSKREDAQALRFEVTERTHRGFAGRWGKEKDQKLRSFLRFQAPC 316

Query: 306 VLQNNREMPNDKGGMDYFTGLFLCRPTGQGKSMLIVRFGSTRSSLLVKLLPTWFMHQNAC 365
           VL N+ E  ++KG   YFTGLFLCRPTGQGKSM+IVRFG+T+   + K++P W+ HQNAC
Sbjct: 317 VLSNDIEFADEKGVTQYFTGLFLCRPTGQGKSMIIVRFGATKRPQMAKVIPKWYFHQNAC 376

Query: 366 KVFEQDMGFLSSQNEVLMREKVPTKELYLNLRSSDTFVLEYRRWMDKVGNGMPYYFGHST 425
           K+FEQDMGFLSSQNEVLM+EKVPTKELY+NL+SSDT+V EYR+WMDKVG+GMPYYFGH+T
Sbjct: 377 KIFEQDMGFLSSQNEVLMKEKVPTKELYINLKSSDTWVAEYRKWMDKVGHGMPYYFGHNT 436

Query: 426 ISLPKVPAVVEHAPVGLVAGASASPPAKGGIGTMYAPNLSNRYFRHIIHCRSCSNTIKSF 485
           ISLP+VPAVVEHAP GLVAG SAS PAKGGIGTM+APN++NRYFRH+IHC+ C++ +K+F
Sbjct: 437 ISLPEVPAVVEHAPAGLVAGVSASSPAKGGIGTMHAPNVNNRYFRHVIHCKGCTSVVKNF 496

Query: 486 QTWNKAFSAIGITLVALAILVSGRQWKAVILLSAALCFGGAYACSAIVALNTTNFIRTHR 530
           Q W  A SAI + L ALAIL SGRQWK  +L+SA+LC  G YACS  +A+NTTNFIRTHR
Sbjct: 497 QAWKNALSAISVALTALAILASGRQWKTFLLVSASLCLAGVYACSTAIAMNTTNFIRTHR 549

BLAST of Lsi04G013560 vs. TAIR10
Match: AT2G24820.1 (AT2G24820.1 translocon at the inner envelope membrane of chloroplasts 55-II)

HSP 1 Score: 680.2 bits (1754), Expect = 9.9e-196
Identity = 348/545 (63.85%), Postives = 403/545 (73.94%), Query Frame = 1

Query: 6   PFLSTAPFSYAPKP---TSLTNSLLLHAPSSSSFKLVAGKRRFAVRTRAVAGAETAAQIG 65
           PFLS++       P   T +T + ++H   S+   +    R   +R  AVAG   + Q  
Sbjct: 4   PFLSSSLQLTPTSPILFTKVTPTPIIHNHRSTC-TIPTKPRLRLLRRSAVAGTAVSDQT- 63

Query: 66  DLKDDDGQVVLFGPAIDEEPGGEATAEYDWTEEWYPLYLTRNVPEDAPLGLTVFDKQLVL 125
               + G  VL  P   EE      A+YDWTEEWYPLYLT+NVPEDAPLGLTV+D+Q+VL
Sbjct: 64  ----EGGGDVLLNP---EEEKRVEVADYDWTEEWYPLYLTKNVPEDAPLGLTVYDRQIVL 123

Query: 126 YRDGTGELRCHEDRCPHR-----EVPLVSTSVYRKYFRLK------------LPSDAKIP 185
           Y+DG G LRC+EDRCPHR     E  L+   +   Y   +            LP+ AKIP
Sbjct: 124 YKDGEGTLRCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPASAKIP 183

Query: 186 RSACLRTYEVRDSQGVVWVWMSLEKPPTLKKIPRFENFDRPGFQDISSVHELPYDHSILL 245
           ++AC++TYEV+DSQGVVWVWMS + PP  +K+P FENF RPGF DIS+ HELPYDHSILL
Sbjct: 184 KAACVKTYEVKDSQGVVWVWMSTKTPPNPEKLPWFENFARPGFFDISTTHELPYDHSILL 243

Query: 246 ENLMDPAHIPISHDRTDFTARREDAQPLGFEITERTNRGFAGRWGRAKDQPL-PNFLRFE 305
           ENLMDPAH+PISHDRTDFTA+REDAQPL FE+TER+NRGFAG WGR K+     N LRF+
Sbjct: 244 ENLMDPAHVPISHDRTDFTAKREDAQPLVFEVTERSNRGFAGTWGREKEGGKGSNLLRFD 303

Query: 306 APCVLQNNREMPNDKGGMDYFTGLFLCRPTGQGKSMLIVRFGSTRSSLLVKLLPTWFMHQ 365
           APCVLQNNRE     G  +YF+GLFLCRPTGQGKSMLIVRFG T+ S LV +LP WF HQ
Sbjct: 304 APCVLQNNREFEGKDGVKNYFSGLFLCRPTGQGKSMLIVRFGVTKRSPLVSVLPQWFWHQ 363

Query: 366 NACKVFEQDMGFLSSQNEVLMREKVPTKELYLNLRSSDTFVLEYRRWMDKVGNGMPYYFG 425
           NACKVFEQDMGFLSSQNEVLM+EKVPTK+LYLNL+SSDT+V EYR+WMDKVG+GMPY+FG
Sbjct: 364 NACKVFEQDMGFLSSQNEVLMKEKVPTKDLYLNLKSSDTWVAEYRKWMDKVGHGMPYHFG 423

Query: 426 HSTISLPKVPAVVEHAPVGLVAGASASPPAKGGIGTMYAPNLSNRYFRHIIHCRSCSNTI 485
           H TISLPKVP VVEHAP GL+A  SAS PAKGGIGTM+APNL+NRYFRHIIHCRSCSN I
Sbjct: 424 HRTISLPKVPPVVEHAPAGLIAALSASYPAKGGIGTMHAPNLANRYFRHIIHCRSCSNVI 483

Query: 486 KSFQTWNKAFSAIGITLVALAILVSGRQWKAVILLSAALCFGGAYACSAIVALNTTNFIR 530
           KSF+ W    SA  + L ALAILV  RQWKAV+L SAALC   AY C   + LNT NFIR
Sbjct: 484 KSFELWKNILSATAVALTALAILVVSRQWKAVLLGSAALCSAAAYTCLRAINLNTNNFIR 539

BLAST of Lsi04G013560 vs. TAIR10
Match: AT4G25650.2 (AT4G25650.2 ACD1-like)

HSP 1 Score: 92.0 bits (227), Expect = 1.1e-18
Identity = 105/503 (20.87%), Postives = 177/503 (35.19%), Query Frame = 1

Query: 81  EPGGEATAEYDWTEEWYPLYLTRNVPEDAPLGLTVFDKQLVLYRD-GTGELRCHEDRCPH 140
           EPG +   ++DW   WYP+    ++ +  P G  V    LV++ D    + +  +D CPH
Sbjct: 73  EPGSD---KFDWYANWYPVMPICDLDKKVPHGKKVMGIDLVVWWDRNEKQWKVMDDTCPH 132

Query: 141 --------REVPLVSTSVYRKYFRLKLPSDAK-IPRS------------ACLRTYEVRDS 200
                   R             +      D K IP++            AC+  Y     
Sbjct: 133 RLAPLSDGRIDQWGRLQCVYHGWCFNGSGDCKLIPQAPPDGPPVHTFKQACVAVYPSTVQ 192

Query: 201 QGVVWVWMSLEKPPTLKKI------PRFENFDRPGFQDISSVHELPYDHSILLENLMDPA 260
             ++W W + +  P  K I      P     + P F  +    ++PY + +L+ENLMDPA
Sbjct: 193 HEIIWFWPNSD--PKYKNIIETNKPPYIPELEDPSFTKLMGNRDIPYGYDVLVENLMDPA 252

Query: 261 HIPISHDRTDFTARREDAQPLGFEITERTNRGF----------AGRWGRAKDQPLP---- 320
           H+P +H            +P G  I   +N  F          A +  R   +PL     
Sbjct: 253 HVPYAH-----YGLMRFPKPKGKYIICISNSCFNPFTNLQILLAEKIDREGGKPLEINVK 312

Query: 321 -------------NFLRFEAPCVLQNN--------REMP----NDKGGMD--YFTGLFLC 380
                         +  F APCV +++         E P    +DK  +     + +F+C
Sbjct: 313 KLDNKGFFSKQEWGYSNFIAPCVYRSSTDPLPEQEHEYPAPAASDKAALSKRRLSLIFIC 372

Query: 381 RPTGQGKSMLIVRFGSTRSSLLVKLLPTWFMHQNACKVFEQDMGFLS-SQNEVLMREKVP 440
            P   G+S LI  F       + K++P W  H     + + D+  L   + ++L R    
Sbjct: 373 IPVSPGRSRLIWTFPRNFGVFIDKIVPRWVFHIGQNTILDSDLHLLHVEERKILERGPEN 432

Query: 441 TKELYLNLRSSDTFVLEYRRWMDKVGNGMPYYFG-HSTISLPKVPAVVEHAPVGLVAGAS 500
            ++       SD  V+ +RRW +K       + G      LP  P               
Sbjct: 433 WQKACFIPTKSDANVVTFRRWFNKYSEARVDWRGKFDPFLLPPTPP-------------- 492

Query: 501 ASPPAKGGIGTMYAPNLSNRYFRHIIHCRSCSNTIKSFQTWNKAFSAIGITLVALAILVS 509
                           L +RY+ H+ +C SC    K             + ++ +  ++ 
Sbjct: 493 -------------REQLFDRYWSHVENCSSCKKAHKYLNALEVILQIASVAMIGVMAVLK 538

BLAST of Lsi04G013560 vs. NCBI nr
Match: gi|659104126|ref|XP_008452818.1| (PREDICTED: protein TIC 55, chloroplastic [Cucumis melo])

HSP 1 Score: 877.1 bits (2265), Expect = 1.6e-251
Identity = 431/546 (78.94%), Postives = 468/546 (85.71%), Query Frame = 1

Query: 1   MASLRPFLSTAPFSYAPKPTSLTNSLLLHAPSSSSFKLVAGKRRFAVRTRAVAGAETAAQ 60
           MASLRPFLS +P   AP  T  T+SLL+HAP SSSFKLV+ K RFAVRT AVAGAE A  
Sbjct: 92  MASLRPFLSLSPVFPAPNHTRPTDSLLIHAPFSSSFKLVSAKPRFAVRTAAVAGAEIADP 151

Query: 61  IGDLKDDDGQVVLFGPAIDEEPGGEATAEYDWTEEWYPLYLTRNVPEDAPLGLTVFDKQL 120
           +GDLKDDD QVVL  P   E+P GEATAEY+WTEEWYPLYLTR+VPEDAPLGLTVFDKQL
Sbjct: 152 VGDLKDDDNQVVLLDPTTSEKPDGEATAEYNWTEEWYPLYLTRDVPEDAPLGLTVFDKQL 211

Query: 121 VLYRDGTGELRCHEDRCPHR-----EVPLVSTSVYRKYFRL------------KLPSDAK 180
           VLYRDG GEL CHEDRCPHR     E  L+   +   Y               +LPSDAK
Sbjct: 212 VLYRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPSDAK 271

Query: 181 IPRSACLRTYEVRDSQGVVWVWMSLEKPPTLKKIPRFENFDRPGFQDISSVHELPYDHSI 240
           IPRSACLRTYEVR+SQGVVWVWMSLEKP TLK IP FENFDRPGF+D SS+HELPYDHSI
Sbjct: 272 IPRSACLRTYEVRESQGVVWVWMSLEKPSTLKNIPWFENFDRPGFRDFSSIHELPYDHSI 331

Query: 241 LLENLMDPAHIPISHDRTDFTARREDAQPLGFEITERTNRGFAGRWGRAKDQPLPNFLRF 300
           LLENLMDPAHIPISHDRT + A+REDAQPL FE+TERTNRGFAGRWG AK+QPL NFLRF
Sbjct: 332 LLENLMDPAHIPISHDRTSYPAKREDAQPLAFEVTERTNRGFAGRWGNAKEQPLSNFLRF 391

Query: 301 EAPCVLQNNREMPNDKGGMDYFTGLFLCRPTGQGKSMLIVRFGSTRSSLLVKLLPTWFMH 360
           EAPCVLQNNRE  N KG +DYFTGLFLCRPTGQGKSM+IVRFGST S++ + L P+WF+H
Sbjct: 392 EAPCVLQNNRETTNKKGEIDYFTGLFLCRPTGQGKSMVIVRFGSTASTVRLNLFPSWFLH 451

Query: 361 QNACKVFEQDMGFLSSQNEVLMREKVPTKELYLNLRSSDTFVLEYRRWMDKVGNGMPYYF 420
           QNAC+VFEQDMGFLSSQNEVLM+EKVPTKELYLNLRSSDT+VLEYRRWMDKVG+GMPYYF
Sbjct: 452 QNACRVFEQDMGFLSSQNEVLMKEKVPTKELYLNLRSSDTWVLEYRRWMDKVGHGMPYYF 511

Query: 421 GHSTISLPKVPAVVEHAPVGLVAGASASPPAKGGIGTMYAPNLSNRYFRHIIHCRSCSNT 480
           GHSTISLPK+PAV+EHAP GLVAG +ASPPAKGGIGTM+APNL+NRYFRHIIHCRSCSN 
Sbjct: 512 GHSTISLPKLPAVIEHAPAGLVAGTAASPPAKGGIGTMHAPNLNNRYFRHIIHCRSCSNV 571

Query: 481 IKSFQTWNKAFSAIGITLVALAILVSGRQWKAVILLSAALCFGGAYACSAIVALNTTNFI 530
           IKSFQTWNKA SAI ITL+ALAILV+GRQWKA+ LLSAAL FGGAYACSA VALNT NFI
Sbjct: 572 IKSFQTWNKALSAIAITLIALAILVNGRQWKALSLLSAALFFGGAYACSAAVALNTDNFI 631

BLAST of Lsi04G013560 vs. NCBI nr
Match: gi|449455677|ref|XP_004145578.1| (PREDICTED: protein TIC 55, chloroplastic [Cucumis sativus])

HSP 1 Score: 864.4 bits (2232), Expect = 1.0e-247
Identity = 429/547 (78.43%), Postives = 464/547 (84.83%), Query Frame = 1

Query: 1   MASLRPFLSTAPFSYAPKPTSLTNSLLLHAPS-SSSFKLVAGKRRFAVRTRAVAGAETAA 60
           MASLRPFLS +P S AP PT  TN  LLH+P  SSSFKLVA K RFAVRT AVAGA+ A 
Sbjct: 1   MASLRPFLSLSPVSPAPNPTRPTNFHLLHSPLFSSSFKLVATKTRFAVRTAAVAGAQIAD 60

Query: 61  QIGDLKDDDGQVVLFGPAIDEEPGGEATAEYDWTEEWYPLYLTRNVPEDAPLGLTVFDKQ 120
            +     DD QVVL GP   E+P  EATAEY+WTEEWYPLYLT++VPEDAPLGLTVFDKQ
Sbjct: 61  PV-----DDNQVVLLGPTTSEKPDDEATAEYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQ 120

Query: 121 LVLYRDGTGELRCHEDRCPHR-----EVPLVSTSVYRKYFRL------------KLPSDA 180
           LVLYRDG GEL CHEDRCPHR     E  L+   +   Y               +LPSDA
Sbjct: 121 LVLYRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPSDA 180

Query: 181 KIPRSACLRTYEVRDSQGVVWVWMSLEKPPTLKKIPRFENFDRPGFQDISSVHELPYDHS 240
           KIPRSACLRTYEVRDSQGVVWVWMSLEKP TLK IP FENFDRPGF+D SS+HELPYDHS
Sbjct: 181 KIPRSACLRTYEVRDSQGVVWVWMSLEKPSTLKSIPWFENFDRPGFRDFSSIHELPYDHS 240

Query: 241 ILLENLMDPAHIPISHDRTDFTARREDAQPLGFEITERTNRGFAGRWGRAKDQPLPNFLR 300
           ILLENLMDPAHIPISHDRT + A+REDAQPL FE+TERTNRGFAGRWG AKDQPL NFLR
Sbjct: 241 ILLENLMDPAHIPISHDRTSYPAKREDAQPLDFEVTERTNRGFAGRWGNAKDQPLSNFLR 300

Query: 301 FEAPCVLQNNREMPNDKGGMDYFTGLFLCRPTGQGKSMLIVRFGSTRSSLLVKLLPTWFM 360
           FEAPCVLQNNRE PN KGG+DYFTGLFLCRPTGQGKSM+IVRFGST S++ + L P WF+
Sbjct: 301 FEAPCVLQNNRETPNKKGGIDYFTGLFLCRPTGQGKSMVIVRFGSTASTVRLNLFPAWFL 360

Query: 361 HQNACKVFEQDMGFLSSQNEVLMREKVPTKELYLNLRSSDTFVLEYRRWMDKVGNGMPYY 420
           HQNAC+VFEQDMGFLSSQNEVLM+EKVPTKELYLNLRSSDT+VLEYRRWMDKVG+GMPYY
Sbjct: 361 HQNACRVFEQDMGFLSSQNEVLMKEKVPTKELYLNLRSSDTWVLEYRRWMDKVGHGMPYY 420

Query: 421 FGHSTISLPKVPAVVEHAPVGLVAGASASPPAKGGIGTMYAPNLSNRYFRHIIHCRSCSN 480
           FGHSTISLPK+PAV+EHAP G+VAG +ASPPAKGGIGTM+APNLSNRYFRHIIHCRSCSN
Sbjct: 421 FGHSTISLPKLPAVIEHAPAGIVAGTAASPPAKGGIGTMHAPNLSNRYFRHIIHCRSCSN 480

Query: 481 TIKSFQTWNKAFSAIGITLVALAILVSGRQWKAVILLSAALCFGGAYACSAIVALNTTNF 530
            IKSFQTWNKA SAI ITL+ALAILV+GRQWKA+ LLSAAL FGGAYACSA +ALNT NF
Sbjct: 481 VIKSFQTWNKALSAIAITLIALAILVNGRQWKALSLLSAALFFGGAYACSAALALNTDNF 540

BLAST of Lsi04G013560 vs. NCBI nr
Match: gi|1009134110|ref|XP_015884267.1| (PREDICTED: protein TIC 55, chloroplastic [Ziziphus jujuba])

HSP 1 Score: 741.1 bits (1912), Expect = 1.3e-210
Identity = 369/543 (67.96%), Postives = 424/543 (78.08%), Query Frame = 1

Query: 5   RPFLSTAPFSYAPKPTSLTNSLLLHAPSSSSFKLVAGKRRFAVRTRAVAGAETAAQIGDL 64
           R  LS++  S   KP S T   L    S+    L + K +   +  A+A  + +    D 
Sbjct: 11  RILLSSSLLSPTSKPPSPTTKFLFSISSNPLKPLPSRKNQ--TQCGAIADIKASPPFDDH 70

Query: 65  KDDDGQVVLFGPAIDEEPGGEA-TAEYDWTEEWYPLYLTRNVPEDAPLGLTVFDKQLVLY 124
           KD     VL    I+EE  GE   A+YDWTEEWYPLYLTR+VPEDAPLGLTVFDKQLVLY
Sbjct: 71  KD-----VLLDATIEEEHRGERDVADYDWTEEWYPLYLTRDVPEDAPLGLTVFDKQLVLY 130

Query: 125 RDGTGELRCHEDRCPHR-----EVPLVSTSVYRKYFRL------------KLPSDAKIPR 184
           RDG GELRC++DRCPHR     E  L+   +   Y               +LP+DAKIPR
Sbjct: 131 RDGAGELRCYQDRCPHRLAKLSEGQLIDGKLECLYHGWQFQGDGKCVKIPQLPADAKIPR 190

Query: 185 SACLRTYEVRDSQGVVWVWMSLEKPPTLKKIPRFENFDRPGFQDISSVHELPYDHSILLE 244
           SACL+TYEVRDSQGVVWVWMS + PP   K+P FENF RPGFQDIS++HELPYDHSILLE
Sbjct: 191 SACLKTYEVRDSQGVVWVWMSQKTPPNPSKLPWFENFARPGFQDISTIHELPYDHSILLE 250

Query: 245 NLMDPAHIPISHDRTDFTARREDAQPLGFEITERTNRGFAGRWGRAKDQPLPNFLRFEAP 304
           NLMDPAHIPISHDRTD+TA+REDAQ LGFE+TER+NRGFAG WG+A DQPLPNFLRFEAP
Sbjct: 251 NLMDPAHIPISHDRTDWTAKREDAQALGFEVTERSNRGFAGWWGKATDQPLPNFLRFEAP 310

Query: 305 CVLQNNREMPNDKGGMDYFTGLFLCRPTGQGKSMLIVRFGSTRSSLLVKLLPTWFMHQNA 364
           CVLQNNRE+ +  G  DYFTGLFLCRP+GQGKSMLIVRFG T+ S L+KL P W+ HQNA
Sbjct: 311 CVLQNNREIVDKNGQKDYFTGLFLCRPSGQGKSMLIVRFGGTKRSPLIKLFPQWYFHQNA 370

Query: 365 CKVFEQDMGFLSSQNEVLMREKVPTKELYLNLRSSDTFVLEYRRWMDKVGNGMPYYFGHS 424
           CKVFEQDMGFLSSQNEVL++EKVPTKELYLNLRSSDT+V EYR+WMDKVG+GMPY+FGHS
Sbjct: 371 CKVFEQDMGFLSSQNEVLIKEKVPTKELYLNLRSSDTWVAEYRKWMDKVGHGMPYHFGHS 430

Query: 425 TISLPKVPAVVEHAPVGLVAGASASPPAKGGIGTMYAPNLSNRYFRHIIHCRSCSNTIKS 484
           TI LPK PAVVEHAP GLVAG SAS PAKGGIG+M+ PNL+NRYFRH+IHC+ C N +K+
Sbjct: 431 TILLPKEPAVVEHAPAGLVAGVSASAPAKGGIGSMHVPNLNNRYFRHVIHCKGCRNAVKA 490

Query: 485 FQTWNKAFSAIGITLVALAILVSGRQWKAVILLSAALCFGGAYACSAIVALNTTNFIRTH 530
           FQTW  A SAI I +VA+AILVSGRQWKA++L++AA C GGAYACSA +ALNTTNFIR H
Sbjct: 491 FQTWKIALSAIAIAMVAVAILVSGRQWKALLLVAAAACSGGAYACSAAIALNTTNFIRIH 546

BLAST of Lsi04G013560 vs. NCBI nr
Match: gi|567916472|ref|XP_006450242.1| (hypothetical protein CICLE_v10007965mg [Citrus clementina])

HSP 1 Score: 723.0 bits (1865), Expect = 3.8e-205
Identity = 352/518 (67.95%), Postives = 408/518 (78.76%), Query Frame = 1

Query: 31  PSSSSFKLVAGKRRFAVRTRAVAGAETAAQIGDLKDDDGQVVLFGPAIDEEPGGEA-TAE 90
           P +++  +   K   + +  AV    + + +GD K      VL GPA  EE  GE   A+
Sbjct: 23  PPTTNVIISLNKTPKSRKCHAVTDRSSTSTVGDHK------VLVGPASAEERRGERQVAD 82

Query: 91  YDWTEEWYPLYLTRNVPEDAPLGLTVFDKQLVLYRDGTGELRCHEDRCPHR-----EVPL 150
           YDWTEEWYPLYLT++VP+DAPLGLTVFD+Q+VLY+DG GELRC++DRCPHR     E  L
Sbjct: 83  YDWTEEWYPLYLTKDVPDDAPLGLTVFDQQIVLYKDGNGELRCYQDRCPHRLAKLSEGQL 142

Query: 151 VSTSVYRKYFRLK------------LPSDAKIPRSACLRTYEVRDSQGVVWVWMSLEKPP 210
           +   +   Y   +            LP+DAKIPRSAC+RTYEV++SQGVVWVWMS + PP
Sbjct: 143 IDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPRSACVRTYEVKESQGVVWVWMSQKTPP 202

Query: 211 TLKKIPRFENFDRPGFQDISSVHELPYDHSILLENLMDPAHIPISHDRTDFTARREDAQP 270
              K+P FENF RPGFQD+S++HELPYDHSILLENLMDPAHIPISHDRTD+TA+REDAQP
Sbjct: 203 NPDKLPWFENFARPGFQDVSTIHELPYDHSILLENLMDPAHIPISHDRTDWTAKREDAQP 262

Query: 271 LGFEITERTNRGFAGRWGRAKDQPLPNFLRFEAPCVLQNNREMPNDKGG-MDYFTGLFLC 330
           LGFE+TERT+RGFAGRWG+ KD+PLPNFLRFEAPCVLQNNRE+ + K G   YFTGLFLC
Sbjct: 263 LGFEVTERTDRGFAGRWGKEKDEPLPNFLRFEAPCVLQNNRELVDSKTGEKRYFTGLFLC 322

Query: 331 RPTGQGKSMLIVRFGSTRSSLLVKLLPTWFMHQNACKVFEQDMGFLSSQNEVLMREKVPT 390
           RPTGQGKSMLIVRFG+T+ S L KL P W+ HQNA KVFEQDMGFLSSQNEVLM+EKVPT
Sbjct: 323 RPTGQGKSMLIVRFGATKRSPLAKLFPKWYFHQNASKVFEQDMGFLSSQNEVLMKEKVPT 382

Query: 391 KELYLNLRSSDTFVLEYRRWMDKVGNGMPYYFGHSTISLPKVPAVVEHAPVGLVAGASAS 450
           KELYLNLRSSDT+V EYR+WMDKVG+GMPY+FGHSTISLPKVPAVVEHAP GLVAG SAS
Sbjct: 383 KELYLNLRSSDTWVAEYRKWMDKVGHGMPYHFGHSTISLPKVPAVVEHAPAGLVAGVSAS 442

Query: 451 PPAKGGIGTMYAPNLSNRYFRHIIHCRSCSNTIKSFQTWNKAFSAIGITLVALAILVSGR 510
            PAKG IGTM+APNL+NRYFRH+IHC+ CS+ IK+F TW  + S +   L  LAIL SGR
Sbjct: 443 SPAKGAIGTMHAPNLANRYFRHVIHCKGCSSVIKAFSTWKNSLSVVAAALTVLAILASGR 502

Query: 511 QWKAVILLSAALCFGGAYACSAIVALNTTNFIRTHRRL 530
           QWKA  L SA+LC  G YACS  +A NTTNFIRTHRRL
Sbjct: 503 QWKAFCLASASLCLAGVYACSTAIATNTTNFIRTHRRL 534

BLAST of Lsi04G013560 vs. NCBI nr
Match: gi|357465983|ref|XP_003603276.1| (translocon at the inner envelope membrane ofs protein 55-II [Medicago truncatula])

HSP 1 Score: 722.6 bits (1864), Expect = 4.9e-205
Identity = 361/554 (65.16%), Postives = 428/554 (77.26%), Query Frame = 1

Query: 1   MASLRPFL--STAPFSYAPKPTSLTNSLLLHAPSSS-SFKLVAG----KRRFAVRTRAVA 60
           MA + PFL  +   F+    P      LL  +PSSS SF L  G    +R+ A    A A
Sbjct: 1   MALVNPFLLPTKTHFALPVSPPPSKQILLWTSPSSSFSFNLNKGLSSSRRKHAGYLPATA 60

Query: 61  GAETAAQIGDLKDDDGQVVLFGPAIDEEPGGEAT-AEYDWTEEWYPLYLTRNVPEDAPLG 120
             +TA+ + D+++D  Q VL GP+ ++E  GE   A YDW EEWYPLYLT+NVP+DAPLG
Sbjct: 61  DVKTASTLLDVEED--QKVLVGPSSEQERRGERELAAYDWKEEWYPLYLTKNVPDDAPLG 120

Query: 121 LTVFDKQLVLYRDGTGELRCHEDRCPHR-----EVPLVSTSVYRKYFRL----------- 180
           LTVFDK LVL++DG  + +C+EDRCPHR     E  L+   +   Y              
Sbjct: 121 LTVFDKNLVLFKDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKI 180

Query: 181 -KLPSDAKIPRSACLRTYEVRDSQGVVWVWMSLEKPPTLKKIPRFENFDRPGFQDISSVH 240
            +LP+DAKIPRSAC++TYEVRDSQGV+WVWMS + PP + KIP FENF RPGFQD+S+ H
Sbjct: 181 PQLPADAKIPRSACVKTYEVRDSQGVIWVWMSPKVPPNVSKIPWFENFARPGFQDVSTTH 240

Query: 241 ELPYDHSILLENLMDPAHIPISHDRTDFTARREDAQPLGFEITERTNRGFAGRWGRAKDQ 300
           ELPYDHSILLENLMDPAHIPISHDRTD+TA+REDAQPL FE+TERT+RGFAG WGR KD 
Sbjct: 241 ELPYDHSILLENLMDPAHIPISHDRTDWTAKREDAQPLLFEVTERTDRGFAGWWGREKDG 300

Query: 301 PLPNFLRFEAPCVLQNNREMPNDKGGMDYFTGLFLCRPTGQGKSMLIVRFGSTRSSLLVK 360
             PNFLRFEAPCVLQNNRE+ +  G ++YF+GLFLCRPTGQGKSMLIVRFG T+ S +VK
Sbjct: 301 TKPNFLRFEAPCVLQNNREIVDKNGEINYFSGLFLCRPTGQGKSMLIVRFGGTKRSPIVK 360

Query: 361 LLPTWFMHQNACKVFEQDMGFLSSQNEVLMREKVPTKELYLNLRSSDTFVLEYRRWMDKV 420
           L P W+ HQNA KVFEQDMGFLSSQNE+L++EKVPTKELYLNL+SSDT+V EYR+WMDKV
Sbjct: 361 LFPQWYFHQNASKVFEQDMGFLSSQNEILLKEKVPTKELYLNLKSSDTWVAEYRKWMDKV 420

Query: 421 GNGMPYYFGHSTISLPKVPAVVEHAPVGLVAGASASPPAKGGIGTMYAPNLSNRYFRHII 480
           G+GMPY+FGH+TISLPK PAV+EHAP GL+AG SAS PAKGGIGTM+APNL+NRYFRH+I
Sbjct: 421 GHGMPYHFGHNTISLPKEPAVIEHAPAGLIAGLSASSPAKGGIGTMHAPNLANRYFRHVI 480

Query: 481 HCRSCSNTIKSFQTWNKAFSAIGITLVALAILVSGRQWKAVILLSAALCFGGAYACSAIV 530
           HC+ C   +K+FQTW  A SA+ + L ALAILV+GRQWKA++L SAALC  G YACS  V
Sbjct: 481 HCKGCRTAVKAFQTWKNALSAVVVALAALAILVTGRQWKAILLASAALCSVGVYACSTAV 540

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TIC55_PEA9.6e-20163.27Protein TIC 55, chloroplastic OS=Pisum sativum GN=TIC55 PE=1 SV=1[more]
TIC55_ARATH1.7e-19463.85Protein TIC 55, chloroplastic OS=Arabidopsis thaliana GN=TIC55 PE=1 SV=1[more]
CAO_CHLRE1.2e-1723.26Chlorophyllide a oxygenase, chloroplastic OS=Chlamydomonas reinhardtii GN=CAO PE... [more]
PTC52_ARATH2.0e-1720.87Protochlorophyllide-dependent translocon component 52, chloroplastic OS=Arabidop... [more]
TSAM1_COMTE1.8e-1023.94Toluene-4-sulfonate monooxygenase system iron-sulfur subunit TsaM1 OS=Comamonas ... [more]
Match NameE-valueIdentityDescription
A0A0A0L0A9_CUCSA7.3e-24878.43Uncharacterized protein OS=Cucumis sativus GN=Csa_4G651770 PE=4 SV=1[more]
V4UBR3_9ROSI2.6e-20567.95Uncharacterized protein OS=Citrus clementina GN=CICLE_v10007965mg PE=4 SV=1[more]
G7J9S7_MEDTR3.4e-20565.16Translocon at the inner envelope membrane ofs protein 55-II OS=Medicago truncatu... [more]
A0A067FW11_CITSI4.5e-20567.76Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g039074mg PE=4 SV=1[more]
B9IL92_POPTR2.2e-20463.84Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0018s02890g PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G24820.19.9e-19663.85 translocon at the inner envelope membrane of chloroplasts 55-II[more]
AT4G25650.21.1e-1820.87 ACD1-like[more]
Match NameE-valueIdentityDescription
gi|659104126|ref|XP_008452818.1|1.6e-25178.94PREDICTED: protein TIC 55, chloroplastic [Cucumis melo][more]
gi|449455677|ref|XP_004145578.1|1.0e-24778.43PREDICTED: protein TIC 55, chloroplastic [Cucumis sativus][more]
gi|1009134110|ref|XP_015884267.1|1.3e-21067.96PREDICTED: protein TIC 55, chloroplastic [Ziziphus jujuba][more]
gi|567916472|ref|XP_006450242.1|3.8e-20567.95hypothetical protein CICLE_v10007965mg [Citrus clementina][more]
gi|357465983|ref|XP_003603276.1|4.9e-20565.16translocon at the inner envelope membrane ofs protein 55-II [Medicago truncatula... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
GO:00515372 iron, 2 sulfur cluster binding
GO:0010277chlorophyllide a oxygenase [overall] activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: INTERPRO
TermDefinition
IPR017941Rieske_2Fe-2S
IPR013626PaO
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0006810 transport
biological_process GO:0045036 protein targeting to chloroplast
biological_process GO:0070988 demethylation
cellular_component GO:0009507 chloroplast
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009528 plastid inner membrane
cellular_component GO:0009941 chloroplast envelope
cellular_component GO:0005575 cellular_component
molecular_function GO:0051537 2 iron, 2 sulfur cluster binding
molecular_function GO:0010277 chlorophyllide a oxygenase [overall] activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0018489 vanillate monooxygenase activity
molecular_function GO:0016491 oxidoreductase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G013560.1Lsi04G013560.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013626Pheophorbide a oxygenasePFAMPF08417PaOcoord: 283..368
score: 1.1
IPR017941Rieske [2Fe-2S] iron-sulphur domainGENE3DG3DSA:2.102.10.10coord: 94..193
score: 1.4
IPR017941Rieske [2Fe-2S] iron-sulphur domainPFAMPF00355Rieskecoord: 95..146
score: 8.
IPR017941Rieske [2Fe-2S] iron-sulphur domainPROFILEPS51296RIESKEcoord: 96..184
score: 19
IPR017941Rieske [2Fe-2S] iron-sulphur domainunknownSSF50022ISP domaincoord: 86..187
score: 3.14
NoneNo IPR availablePANTHERPTHR21266IRON-SULFUR DOMAIN CONTAINING PROTEINcoord: 438..529
score: 8.2E-285coord: 5..412
score: 8.2E
NoneNo IPR availablePANTHERPTHR21266:SF29PROTEIN TIC 55, CHLOROPLASTICcoord: 438..529
score: 8.2E-285coord: 5..412
score: 8.2E
NoneNo IPR availableunknownSSF55961Bet v1-likecoord: 180..398
score: 1.92