Lsi04G010440 (gene) Bottle gourd (USVL1VR-Ls)

BLAST of Lsi04G010440 vs. Swiss-Prot
Match: S38A2_HUMAN (Sodium-coupled neutral amino acid transporter 2 OS=Homo sapiens GN=SLC38A2 PE=1 SV=2)

HSP 1 Score: 108.2 bits (269), Expect = 2.3e-22
Identity = 114/431 (26.45%), Postives = 206/431 (47.80%), Query Frame = 1

Query: 20  TASVSGAVFNVSTSIIGAGIMSIPFTLKVLGIIPAVVLIMLVAFMTDLSVELLLRFTHSA 79
           T S   +VFN+S +I+G+GI+ + + +   GI   ++L+  V+  +  SV LLL+  +  
Sbjct: 70  TTSFGMSVFNLSNAIVGSGILGLSYAMANTGIALFIILLTFVSIFSLYSVHLLLKTANEG 129

Query: 80  ESTTYAGVMKESFGPIGSVATQICVMITNLGCLIMYLIIIGDVLSGNKGGEEEVHLGVLQ 139
            S  Y  +  ++FG +G +A    + + N+G +  YL I+          + E+ L V+Q
Sbjct: 130 GSLLYEQLGYKAFGLVGKLAASGSITMQNIGAMSSYLFIV----------KYELPL-VIQ 189

Query: 140 QWFGIQ-----WWNSREFSILFTVVFILLPLVLYQRVDSLRFSSFVSVVLAVVF--VGIS 199
               I+     W+ +  + +L   + ++LPL L++ +  L ++S +S++  V F  V I 
Sbjct: 190 ALTNIEDKTGLWYLNGNYLVLLVSLVVILPLSLFRNLGYLGYTSGLSLLCMVFFLIVVIC 249

Query: 200 SVMAIMAMVEGK-----------TKRTRLVPEVEDETS--------FF----ELFTAVPV 259
               +   VE             T+ T LVP +    +        +F    +   AVP+
Sbjct: 250 KKFQVPCPVEAALIINETINTTLTQPTALVPALSHNVTENDSCRPHYFIFNSQTVYAVPI 309

Query: 260 IVTAFTFHFNVHPISFEL--RNSRDMMTAVRVALLLCAIIYFTIGIFGYMLFGESLMSDI 319
           ++ +F  H  V PI  EL  R+ R MM   +++     ++Y    +FGY+ F E + S++
Sbjct: 310 LIFSFVCHPAVLPIYEELKDRSRRRMMNVSKISFFAMFLMYLLAALFGYLTFYEHVESEL 369

Query: 320 LTNFDQTFGSSTGALLNDIVRLSYAFHLMLVYPLLNFSLRFNINELLFPYKPPLASDTTR 379
           L  +    G+    +L  IVRL+    + L  P++ F +R ++  LL   K        R
Sbjct: 370 LHTYSSILGTD---ILLLIVRLAVLMAVTLTVPVVIFPIRSSVTHLLCASKD---FSWWR 429

Query: 380 FLTITMALLLFSYLAAIAFPNIWSIFQFMGSTSAACLAFIFPGAIALRDVNGISTKRDKM 419
              IT+++L F+ L  I  P I  IF F+G+++A+ L FI P A  ++ V     K  + 
Sbjct: 430 HSLITVSILAFTNLLVIFVPTIRDIFGFIGASAASMLIFILPSAFYIKLVKKEPMKSVQK 483

BLAST of Lsi04G010440 vs. Swiss-Prot
Match: S38A2_PANPA (Sodium-coupled neutral amino acid transporter 2 OS=Pan paniscus GN=SLC38A2 PE=3 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 2.3e-22
Identity = 114/431 (26.45%), Postives = 206/431 (47.80%), Query Frame = 1

Query: 20  TASVSGAVFNVSTSIIGAGIMSIPFTLKVLGIIPAVVLIMLVAFMTDLSVELLLRFTHSA 79
           T S   +VFN+S +I+G+GI+ + + +   GI   ++L+  V+  +  SV LLL+  +  
Sbjct: 70  TTSFGMSVFNLSNAIVGSGILGLSYAMANTGIALFIILLTFVSIFSLYSVHLLLKTANEG 129

Query: 80  ESTTYAGVMKESFGPIGSVATQICVMITNLGCLIMYLIIIGDVLSGNKGGEEEVHLGVLQ 139
            S  Y  +  ++FG +G +A    + + N+G +  YL I+          + E+ L V+Q
Sbjct: 130 GSLLYEQLGYKAFGLVGKLAASGSITMQNIGAMSSYLFIV----------KYELPL-VIQ 189

Query: 140 QWFGIQ-----WWNSREFSILFTVVFILLPLVLYQRVDSLRFSSFVSVVLAVVF--VGIS 199
               I+     W+ +  + +L   + ++LPL L++ +  L ++S +S++  V F  V I 
Sbjct: 190 ALTNIEDKTGLWYLNGNYLVLLVSLVVILPLSLFRNLGYLGYTSGLSLLCMVFFLIVVIC 249

Query: 200 SVMAIMAMVEGK-----------TKRTRLVPEVEDETS--------FF----ELFTAVPV 259
               +   VE             T+ T LVP +    +        +F    +   AVP+
Sbjct: 250 KKFQVPCPVEAALIINETINTTLTQPTALVPALSHNVTENDSCRPHYFIFNSQTVYAVPI 309

Query: 260 IVTAFTFHFNVHPISFEL--RNSRDMMTAVRVALLLCAIIYFTIGIFGYMLFGESLMSDI 319
           ++ +F  H  V PI  EL  R+ R MM   +++     ++Y    +FGY+ F E + S++
Sbjct: 310 LIFSFVCHPAVLPIYEELKDRSRRRMMNVSKISFFAMFLMYLLAALFGYLTFYEHVESEL 369

Query: 320 LTNFDQTFGSSTGALLNDIVRLSYAFHLMLVYPLLNFSLRFNINELLFPYKPPLASDTTR 379
           L  +    G+    +L  IVRL+    + L  P++ F +R ++  LL   K        R
Sbjct: 370 LHTYSSILGTD---ILLLIVRLAVLMAVTLTVPVVIFPIRSSVTHLLCASKD---FSWWR 429

Query: 380 FLTITMALLLFSYLAAIAFPNIWSIFQFMGSTSAACLAFIFPGAIALRDVNGISTKRDKM 419
              IT+++L F+ L  I  P I  IF F+G+++A+ L FI P A  ++ V     K  + 
Sbjct: 430 HSLITVSILAFTNLLVIFVPTIRDIFGFIGASAASMLIFILPSAFYIKLVKKEPMKSVQK 483

BLAST of Lsi04G010440 vs. Swiss-Prot
Match: S38A1_RAT (Sodium-coupled neutral amino acid transporter 1 OS=Rattus norvegicus GN=Slc38a1 PE=1 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 8.6e-22
Identity = 119/418 (28.47%), Postives = 196/418 (46.89%), Query Frame = 1

Query: 20  TASVSGAVFNVSTSIIGAGIMSIPFTLKVLGIIPAVVLIMLVAFMTDLSVELLLRFTHSA 79
           T S+  +VFN+S +I+G+GI+ + F L   GI+  ++L+  V  ++  S+ LLL  +   
Sbjct: 70  TTSLGMSVFNLSNAIMGSGILGLAFALANTGILLFLILLTSVTLLSIYSINLLLICSKET 129

Query: 80  ESTTYAGVMKESFGPIGSVATQICVMITNLGCLIMYLIIIGDVLSG---NKGGEEEVHLG 139
               Y  + ++ FG  G +       + N G ++ YL I+ + L     +  GEEE    
Sbjct: 130 GCMVYEKLGEQVFGTTGKLVIFGATSLQNTGAMLSYLFIVKNELPSAIKSLMGEEETFSA 189

Query: 140 VLQQWFGIQWWNSREFSILFTVVFILLPLVLYQRVDSLRFSSFVSVVLAVVF--VGISSV 199
                    W+      ++     I+LPL L + +  L ++S  S+   V F  V I   
Sbjct: 190 ---------WYVDGRVLVVMVTFGIILPLCLLKNLGYLGYTSGFSLSCMVFFLIVVIYKK 249

Query: 200 MAIMAMVEGKTKRTRLVPEVEDETSFFELFT-----AVPVIVTAFTFHFNVHPISFEL-- 259
             I  M  G+   T      +  T  +  F      A+P I  AF  H +V PI  EL  
Sbjct: 250 FQIPCM-NGEQNSTVSANVTDACTPKYVTFNSKTVYALPTIAFAFVCHPSVLPIYSELKD 309

Query: 260 RNSRDMMTAVRVALLLCAIIYFTIGIFGYMLFGESLMSDILTNFDQTFGSSTGALLNDIV 319
           R+ + M     ++     ++YF   IFGY+ F E + SD+L  +      STG +L   V
Sbjct: 310 RSQKKMQMVSNISFFAMFVMYFLTAIFGYLTFYEKVQSDLLHKY-----QSTGDILILTV 369

Query: 320 RLSYAFHLMLVYPLLNFSLRFNINELLFPYKPPLASDTTRFLTITMALLLFSYLAAIAFP 379
           RL+    ++L  P+L F++R ++ EL    K  L     R + +T+ LL+   L  I  P
Sbjct: 370 RLAVIVAVILTVPVLFFTVRSSLFELAKKTKFHLC----RHVLVTIILLVIINLLVIFIP 429

Query: 380 NIWSIFQFMGSTSAACLAFIFPGAIALRDVNGISTKRDKMV-ASIMVTLAVVTSIIAI 425
           ++  IF  +G TSA  L FI P ++ L+  N    K  + + A++ + L V+ S+I+I
Sbjct: 430 SMKDIFGVVGVTSANMLIFILPSSLYLKITNQDGDKNTQRIWAALFLALGVLFSLISI 468

BLAST of Lsi04G010440 vs. Swiss-Prot
Match: S38A2_XENTR (Sodium-coupled neutral amino acid transporter 2 OS=Xenopus tropicalis GN=slc38a2 PE=2 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 2.5e-21
Identity = 113/432 (26.16%), Postives = 203/432 (46.99%), Query Frame = 1

Query: 20  TASVSGAVFNVSTSIIGAGIMSIPFTLKVLGIIPAVVLIMLVAFMTDLSVELLLRFTHSA 79
           T S   +VFN+S +I+G+GI+ + + +   GI   ++L++ V   +  S+ LLL+  +  
Sbjct: 66  TTSFGMSVFNLSNAIVGSGILGLSYAMANTGIALFMILLVFVTVFSLYSIHLLLKTANEG 125

Query: 80  ESTTYAGVMKESFGPIGSVATQICVMITNLGCLIMYLIIIGDVLSGNKGGEEEVHLGVLQ 139
            S  Y  +  ++FG  G +A    V + N+G +  YL I+          + E+ L V++
Sbjct: 126 GSLLYEQLGLKAFGIPGKLAASGSVTLQNIGAMSSYLYIV----------KYELPL-VIK 185

Query: 140 QWFGI-----QWWNSREFSILFTVVFILLPLVLYQRVDSLRFSSFVSVVLAVVFV----- 199
               I     +W+ + ++ ++   + I+LPL L + +  L ++S  S +  V F+     
Sbjct: 186 ALMDIKESNGEWYLNGDYLVIMVSLAIILPLSLLRNLGYLGYTSGFSPLCMVFFLIVVIY 245

Query: 200 ---GISSVMAIMAMVEGKTKRTRLV-PEVEDETSFFELFT-------AVPVIVTAFTFHF 259
               I   +  M M    +    +   E +DE    + F        AVP++  +F  H 
Sbjct: 246 KKFEIPCPLEAMNMTSNSSSHDHMAHNETDDEMCKPKYFVFNSQTVYAVPILTFSFVCHP 305

Query: 260 NVHPISFEL--RNSRDMMTAVRVALLLCAIIYFTIGIFGYMLFGESLMSDILTNFDQTFG 319
            V PI  EL  R+ R MM    V+     I+Y    +FGY+ F   +  ++L  + + FG
Sbjct: 306 AVLPIYQELKGRSRRRMMNVSNVSFFAMFIMYLLAALFGYLTFYSKVEPELLHTYSKVFG 365

Query: 320 SSTGALLNDIVRLSYAFHLMLVYPLLNFSLRFNINELLFPYKPPLASDTTRFLTITMALL 379
           +    ++  +VRL+    + L  P++ F +R ++NEL    K        R + IT  +L
Sbjct: 366 A---GVIFVVVRLAVLMAVTLTVPIVIFPIRSSLNELFCSGKD---FAWIRHILITFLIL 425

Query: 380 LFSYLAAIAFPNIWSIFQFMGSTSAACLAFIFPGAIALRDVNGISTKRDKMVASIMVTLA 429
            F+ +  I  P I  IF F+G+++AA L FI P A  +R V   S K  + + +++  + 
Sbjct: 426 AFTNVLVIFVPTIRDIFGFIGASAAAMLVFILPSAFYIRLVKKESMKSVQKIGALLFLIG 480

BLAST of Lsi04G010440 vs. Swiss-Prot
Match: S38A1_MOUSE (Sodium-coupled neutral amino acid transporter 1 OS=Mus musculus GN=Slc38a1 PE=1 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 9.5e-21
Identity = 113/417 (27.10%), Postives = 196/417 (47.00%), Query Frame = 1

Query: 20  TASVSGAVFNVSTSIIGAGIMSIPFTLKVLGIIPAVVLIMLVAFMTDLSVELLLRFTHSA 79
           T S+  +VFN+S +I+G+GI+ + F L   GI+  ++L+  V  ++  S+ LLL  +   
Sbjct: 70  TTSLGMSVFNLSNAIMGSGILGLAFALANTGILLFLILLTSVTLLSIYSINLLLICSKET 129

Query: 80  ESTTYAGVMKESFGPIGSVATQICVMITNLGCLIMYLIIIGDVLSG---NKGGEEEVHLG 139
               Y  + ++ FG  G +       + N G ++ YL I+ + L     +  GEE+    
Sbjct: 130 GCMVYEKLGEQVFGTTGKLVIFGATSLQNTGAMLSYLFIVKNELPSAIKSLMGEEDAFSA 189

Query: 140 VLQQWFGIQWWNSREFSILFTVVFILLPLVLYQRVDSLRFSSFVSVVLAVVFVGI----S 199
                    W+      ++     I+LPL L + +  L ++S  S+   + F+ +     
Sbjct: 190 ---------WYVDGRVLVVMVTFGIILPLCLLKNLGYLGYTSGFSLSCMMFFLIVVIYKK 249

Query: 200 SVMAIMAMVEGKTKRTRLVPEVEDETSFFELFT--AVPVIVTAFTFHFNVHPISFEL--R 259
                M++ +  T    +      +   F   T  A+P I  AF  H +V PI  EL  R
Sbjct: 250 FQTPCMSVEQNSTVSANVTDACTPKYVTFNSKTVYALPTIAFAFVCHPSVLPIYSELKDR 309

Query: 260 NSRDMMTAVRVALLLCAIIYFTIGIFGYMLFGESLMSDILTNFDQTFGSSTGALLNDIVR 319
           + + M     ++     ++YF   IFGY+ F E + SD+L  +      STG +L   VR
Sbjct: 310 SQKKMQMVSNISFFAMFVMYFLTAIFGYLTFYEKVQSDLLHKY-----QSTGDILILTVR 369

Query: 320 LSYAFHLMLVYPLLNFSLRFNINELLFPYKPPLASDTTRFLTITMALLLFSYLAAIAFPN 379
           L+    ++L  P+L F++R ++ EL    K  L     R + +T+ LL+   L  I  P+
Sbjct: 370 LAVIVAVILTVPVLFFTVRSSLFELAKKTKFHLC----RHVLVTIILLIIINLLVIFIPS 429

Query: 380 IWSIFQFMGSTSAACLAFIFPGAIALRDVNGISTKRDKMV-ASIMVTLAVVTSIIAI 425
           +  IF  +G TSA  L FI P ++ L+  N    K  + + A++ + L V+ S+I+I
Sbjct: 430 MKDIFGVVGVTSANMLIFILPSSLYLKITNQDGDKGTQRIWAALFLGLGVLFSLISI 468

BLAST of Lsi04G010440 vs. TrEMBL
Match: A0A0A0KSH6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G218770 PE=4 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 8.7e-215
Identity = 412/439 (93.85%), Postives = 421/439 (95.90%), Query Frame = 1

Query: 1   MSPAPEHVPLLPEMKPAIKTASVSGAVFNVSTSIIGAGIMSIPFTLKVLGIIPAVVLIML 60
           MSP PEHVPLLPEMKP IKTASVSGAVFNVSTSIIGAGIMSIPFTLKVLGIIPAV LIML
Sbjct: 1   MSPPPEHVPLLPEMKPPIKTASVSGAVFNVSTSIIGAGIMSIPFTLKVLGIIPAVALIML 60

Query: 61  VAFMTDLSVELLLRFTHSAESTTYAGVMKESFGPIGSVATQICVMITNLGCLIMYLIIIG 120
           VAFMTDLSVELLLRFTHS+ STTYAGVMKESFG IGSVATQICVMITNLGCLIMYLIIIG
Sbjct: 61  VAFMTDLSVELLLRFTHSSNSTTYAGVMKESFGLIGSVATQICVMITNLGCLIMYLIIIG 120

Query: 121 DVLSGNKGGE--EEVHLGVLQQWFGIQWWNSREFSILFTVVFILLPLVLYQRVDSLRFSS 180
           DVLSGNKGG   EEVH GVLQQWFG  WWNSREFSILFTVVFILLPLVLYQRVDSLRFSS
Sbjct: 121 DVLSGNKGGGGGEEVHFGVLQQWFGNHWWNSREFSILFTVVFILLPLVLYQRVDSLRFSS 180

Query: 181 FVSVVLAVVFVGISSVMAIMAMVEGKTKRTRLVPEVEDETSFFELFTAVPVIVTAFTFHF 240
           FVSVVLAVVFVGISSVMA+MA+VEGKTKRTRLVPEVEDETSFFELFTAVPVIVTAFTFHF
Sbjct: 181 FVSVVLAVVFVGISSVMAVMAIVEGKTKRTRLVPEVEDETSFFELFTAVPVIVTAFTFHF 240

Query: 241 NVHPISFELRNSRDMMTAVRVALLLCAIIYFTIGIFGYMLFGESLMSDILTNFDQTFGSS 300
           NVHPISFELRNSR+MMTAVRVAL+LCAIIYF IGIFGY+LFG+SLMSDILTNFDQTFGSS
Sbjct: 241 NVHPISFELRNSRNMMTAVRVALILCAIIYFAIGIFGYLLFGDSLMSDILTNFDQTFGSS 300

Query: 301 TGA-LLNDIVRLSYAFHLMLVYPLLNFSLRFNINELLFPYKPPLASDTTRFLTITMALLL 360
           TGA LLNDIVRLSYAFHLMLVYPLLNFSLRFNINELLFP +PPLASDTTRF TITMALLL
Sbjct: 301 TGANLLNDIVRLSYAFHLMLVYPLLNFSLRFNINELLFPNRPPLASDTTRFFTITMALLL 360

Query: 361 FSYLAAIAFPNIWSIFQFMGSTSAACLAFIFPGAIALRDVNGISTKRDKMVASIMVTLAV 420
           FSYLAAIAFPNIWSIFQFMGSTSAACLAFIFPGAIALRDVNGISTK DKMVAS MV+LAV
Sbjct: 361 FSYLAAIAFPNIWSIFQFMGSTSAACLAFIFPGAIALRDVNGISTKTDKMVASTMVSLAV 420

Query: 421 VTSIIAIATNINKALRNKF 437
           VTSIIAIATNINKAL NKF
Sbjct: 421 VTSIIAIATNINKALNNKF 439

BLAST of Lsi04G010440 vs. TrEMBL
Match: A0A0A0KQM4_CUCSA (Amino acid transporter OS=Cucumis sativus GN=Csa_5G218780 PE=4 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 1.3e-181
Identity = 348/435 (80.00%), Postives = 389/435 (89.43%), Query Frame = 1

Query: 1   MSPAPEHVPLLPEMKPAIKTASVSGAVFNVSTSIIGAGIMSIPFTLKVLGIIPAVVLIML 60
           MSPAPE VPLLP  KP++KTASVSGAVFNVSTSIIGAGIMSIPF LKVLGIIPA+VLI+ 
Sbjct: 1   MSPAPEQVPLLPAAKPSMKTASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVF 60

Query: 61  VAFMTDLSVELLLRFTHSAESTTYAGVMKESFGPIGSVATQICVMITNLGCLIMYLIIIG 120
           VAF+TD+SVELLLRFTHS +STTYAGVMKESFGPIGS+ATQ+C+MITNLGCLIMY IIIG
Sbjct: 61  VAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIGSIATQVCIMITNLGCLIMYQIIIG 120

Query: 121 DVLSGNKGGEEEVHLGVLQQWFGIQWWNSREFSILFTVVFILLPLVLYQRVDSLRFSSFV 180
           DVLSGNK G + VHLGVLQ+WFG  WWN+REFSILFT+VFIL PLVL++RVDSLRFSSF+
Sbjct: 121 DVLSGNKEGGK-VHLGVLQEWFGHHWWNTREFSILFTLVFILFPLVLFRRVDSLRFSSFI 180

Query: 181 SVVLAVVFVGISSVMAIMAMVEGKTKRTRLVPEVEDETSFFELFTAVPVIVTAFTFHFNV 240
           SVVLAVVF+GISSVMAIMA+VEGKTK TRLVPE+++ETSFF+LFTAVPV+VTAF FHFNV
Sbjct: 181 SVVLAVVFIGISSVMAIMAIVEGKTKSTRLVPELDEETSFFDLFTAVPVLVTAFCFHFNV 240

Query: 241 HPISFELRNSRDMMTAVRVALLLCAIIYFTIGIFGYMLFGESLMSDILTNFDQTFGSSTG 300
           HPIS EL    +M TAVR+ALLLCAI YFTIGIFGY+LFG+SLMSDIL NFD++ G +TG
Sbjct: 241 HPISSELHKPSNMTTAVRIALLLCAIFYFTIGIFGYLLFGDSLMSDILMNFDES-GDTTG 300

Query: 301 ALLNDIVRLSYAFHLMLVYPLLNFSLRFNINELLFPYKPPLASDTTRFLTITMALLLFSY 360
           ALLND+VRLSYA HLMLV+PLLNF LR NI EL FP K    +D  RFL IT+ALL+FSY
Sbjct: 301 ALLNDVVRLSYAVHLMLVFPLLNFPLRSNIIELFFPKKASSGTDQRRFLGITLALLVFSY 360

Query: 361 LAAIAFPNIWSIFQFMGSTSAACLAFIFPGAIALRDVNGISTKRDKMVASIMVTLAVVTS 420
           LAAIAFPNIW IFQFMGSTSAACLAFIFPGAIALRDVNGI+TK+DK+VA  MV LAV TS
Sbjct: 361 LAAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILAVGTS 420

Query: 421 IIAIATNINKALRNK 436
           I+AI+ NIN AL NK
Sbjct: 421 IVAISININNALENK 433

BLAST of Lsi04G010440 vs. TrEMBL
Match: W9QLT8_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_012287 PE=4 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 3.2e-164
Identity = 315/437 (72.08%), Postives = 374/437 (85.58%), Query Frame = 1

Query: 1   MSPA-PEHVPLLPEM-KPAIKTASVSGAVFNVSTSIIGAGIMSIPFTLKVLGIIPAVVLI 60
           MSPA   HVPLLP   K   K AS+SGAVFNV+TSIIGAGIMSIP TLKVLG+IPA VLI
Sbjct: 1   MSPAIGVHVPLLPNHDKQQAKPASISGAVFNVATSIIGAGIMSIPATLKVLGVIPAFVLI 60

Query: 61  MLVAFMTDLSVELLLRFTHSAESTTYAGVMKESFGPIGSVATQICVMITNLGCLIMYLII 120
           ++V  + D+SVE L+RFTHS ++TTY GVMKESFG +GSVATQICVMITN GCLIMYLII
Sbjct: 61  VIVGLLVDISVEFLMRFTHSGDATTYGGVMKESFGQVGSVATQICVMITNFGCLIMYLII 120

Query: 121 IGDVLSGNKGGEEEVHLGVLQQWFGIQWWNSREFSILFTVVFILLPLVLYQRVDSLRFSS 180
           IGDVLSG+K  E  VHLGVLQ+WFGI WWNSREFSILFTVVFI+LPL+L +RV+SLRFSS
Sbjct: 121 IGDVLSGSKP-EGSVHLGVLQEWFGIHWWNSREFSILFTVVFIMLPLLLLRRVESLRFSS 180

Query: 181 FVSVVLAVVFVGISSVMAIMAMVEGKTKRTRLVPEVEDETSFFELFTAVPVIVTAFTFHF 240
            VSV+LAV+FV I SVMAI+A+V+GKTK  RL+PE++ +TSFF+LFTAVPVIVTAFTFHF
Sbjct: 181 AVSVLLAVIFVAICSVMAIIALVQGKTKNPRLIPELDQQTSFFDLFTAVPVIVTAFTFHF 240

Query: 241 NVHPISFELRNSRDMMTAVRVALLLCAIIYFTIGIFGYMLFGESLMSDILTNFDQTFGSS 300
           NVHPI FE+    DM+ AVR+AL+LCA IYF +G+FGY+LFG+S+M+DIL NFD++  ++
Sbjct: 241 NVHPIGFEMGKPSDMVKAVRIALVLCAAIYFAVGLFGYLLFGDSIMTDILVNFDKSSDTA 300

Query: 301 TGALLNDIVRLSYAFHLMLVYPLLNFSLRFNINELLFPYKPPLASDTTRFLTITMALLLF 360
           TG++LND VRLSYA HLMLV+PLLNFSLR NI+E LFP K  L +DT RF+ IT+ LL+F
Sbjct: 301 TGSVLNDAVRLSYALHLMLVFPLLNFSLRANIDEFLFPEKILLVNDTKRFVIITLVLLVF 360

Query: 361 SYLAAIAFPNIWSIFQFMGSTSAACLAFIFPGAIALRDVNGISTKRDKMVASIMVTLAVV 420
           SYLAAIAFPNIW IFQF+GSTSA CLAFIFPGAIALRDVNGIST++D+++A+IM+  AVV
Sbjct: 361 SYLAAIAFPNIWYIFQFLGSTSAVCLAFIFPGAIALRDVNGISTRKDRILATIMIVSAVV 420

Query: 421 TSIIAIATNINKALRNK 436
           TSIIAI+ NI  +  NK
Sbjct: 421 TSIIAISINIYNSFGNK 436

BLAST of Lsi04G010440 vs. TrEMBL
Match: M5XJL3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005957mg PE=4 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 1.2e-163
Identity = 314/436 (72.02%), Postives = 375/436 (86.01%), Query Frame = 1

Query: 1   MSPAP-EHVPLLPEMKPAIKTASVSGAVFNVSTSIIGAGIMSIPFTLKVLGIIPAVVLIM 60
           MSPA    VPLLP  KP ++ A+VSGAVFNVSTSIIGAGIMSIP TLKVLG+IPA +LI+
Sbjct: 1   MSPAAGARVPLLPGNKP-VRRATVSGAVFNVSTSIIGAGIMSIPATLKVLGVIPAFLLIV 60

Query: 61  LVAFMTDLSVELLLRFTHSAESTTYAGVMKESFGPIGSVATQICVMITNLGCLIMYLIII 120
           ++AF+TD+SVE L+RFTHS ES TYAGVM+ESFG +GSVATQICVMITNLGCLIMY III
Sbjct: 61  IIAFLTDVSVEFLMRFTHSGESITYAGVMRESFGQVGSVATQICVMITNLGCLIMYQIII 120

Query: 121 GDVLSGNKGGEEEVHLGVLQQWFGIQWWNSREFSILFTVVFILLPLVLYQRVDSLRFSSF 180
           GDVLSGN G     H GVLQ+WFGIQWWNSR+ +ILFT+VFI LPLVL +RV+SL++SS 
Sbjct: 121 GDVLSGN-GSSGTGHSGVLQEWFGIQWWNSRDIAILFTLVFIFLPLVLLRRVESLKYSSA 180

Query: 181 VSVVLAVVFVGISSVMAIMAMVEGKTKRTRLVPEVEDETSFFELFTAVPVIVTAFTFHFN 240
           ++V+LAVVFVGISS MAI A+ EGKT   +L+P ++ +TSFF+LFTAVPVIVTAFTFHFN
Sbjct: 181 IAVLLAVVFVGISSAMAIYAIFEGKTSNPKLIPSLDKQTSFFDLFTAVPVIVTAFTFHFN 240

Query: 241 VHPISFELRNSRDMMTAVRVALLLCAIIYFTIGIFGYMLFGESLMSDILTNFDQTFGSST 300
           VHPI FEL    DM++AVR++L+LCA IYF+IG+FGY+LFG+++MSDIL NFD++  S+ 
Sbjct: 241 VHPIGFELGKPSDMISAVRISLVLCAAIYFSIGLFGYLLFGDAIMSDILVNFDKSSDSAM 300

Query: 301 GALLNDIVRLSYAFHLMLVYPLLNFSLRFNINELLFPYKPPLASDTTRFLTITMALLLFS 360
           GALLND+VRLSYA HLMLV+PLLNFSLR N++ELLFP KP LA+DT RF+ +T+ LL+FS
Sbjct: 301 GALLNDVVRLSYALHLMLVFPLLNFSLRVNLDELLFPKKPLLATDTRRFVILTLGLLVFS 360

Query: 361 YLAAIAFPNIWSIFQFMGSTSAACLAFIFPGAIALRDVNGISTKRDKMVASIMVTLAVVT 420
           YLAAIAFPNIW IFQF+GSTSA CLAFIFPGAI LRDV+GIST+RDK++A+IMV LAVVT
Sbjct: 361 YLAAIAFPNIWYIFQFLGSTSAVCLAFIFPGAIVLRDVHGISTRRDKIMATIMVFLAVVT 420

Query: 421 SIIAIATNINKALRNK 436
           S IAI+TNI     NK
Sbjct: 421 SAIAISTNIYNFFGNK 434

BLAST of Lsi04G010440 vs. TrEMBL
Match: B9HYI5_POPTR (Amino acid transporter family protein OS=Populus trichocarpa GN=POPTR_0010s18910g PE=4 SV=2)

HSP 1 Score: 581.3 bits (1497), Expect = 1.0e-162
Identity = 311/436 (71.33%), Postives = 374/436 (85.78%), Query Frame = 1

Query: 1   MSPAPE-HVPLLPEMKPAIKTASVSGAVFNVSTSIIGAGIMSIPFTLKVLGIIPAVVLIM 60
           MSPA     PLLPE K   K ASV GAVFNVSTSIIGAGIMSIP TLKVLG+IPA+VLIM
Sbjct: 1   MSPATGIRAPLLPERKEGEKRASVHGAVFNVSTSIIGAGIMSIPATLKVLGVIPALVLIM 60

Query: 61  LVAFMTDLSVELLLRFTHSAESTTYAGVMKESFGPIGSVATQICVMITNLGCLIMYLIII 120
           ++A++ D+SVE LLR+T S ESTTYAGVM+E+FG +GSV  QICVMITNLGCLI+YLIII
Sbjct: 61  IIAWLVDISVEFLLRYTLSGESTTYAGVMREAFGRVGSVTVQICVMITNLGCLIVYLIII 120

Query: 121 GDVLSGNKGGEEEVHLGVLQQWFGIQWWNSREFSILFTVVFILLPLVLYQRVDSLRFSSF 180
           GDVLSGN   +  VHLGVLQ+WFGI WWNSR F++LF V+F++LPLVL++RV+SLRFSS 
Sbjct: 121 GDVLSGNVH-DGSVHLGVLQEWFGIHWWNSRAFALLFVVIFVVLPLVLFRRVESLRFSSA 180

Query: 181 VSVVLAVVFVGISSVMAIMAMVEGKTKRTRLVPEVEDETSFFELFTAVPVIVTAFTFHFN 240
           +SV+LAVVFVGI SVMAI A++EGKTK  RL+P ++++TSFF+LFTA PVIVTAFTFHFN
Sbjct: 181 ISVLLAVVFVGICSVMAIYALIEGKTKSPRLLPHLDNKTSFFDLFTAAPVIVTAFTFHFN 240

Query: 241 VHPISFELRNSRDMMTAVRVALLLCAIIYFTIGIFGYMLFGESLMSDILTNFDQTFGSST 300
           VHPISFEL    DM++AV+++LLLCA IYFTIGI GY+LFGES+++DIL NFDQ+  ++ 
Sbjct: 241 VHPISFELGKPSDMVSAVKISLLLCAGIYFTIGISGYLLFGESIVADILVNFDQSSDTTI 300

Query: 301 GALLNDIVRLSYAFHLMLVYPLLNFSLRFNINELLFPYKPPLASDTTRFLTITMALLLFS 360
           GALLND VRLSYAFHLML +PLLNFSLR NI+ELLFP KP LA+D+ RF+++T+ALL+F+
Sbjct: 301 GALLNDTVRLSYAFHLMLAFPLLNFSLRANIDELLFPKKPLLATDSIRFVSLTLALLVFA 360

Query: 361 YLAAIAFPNIWSIFQFMGSTSAACLAFIFPGAIALRDVNGISTKRDKMVASIMVTLAVVT 420
           YLAAIA PNIW +FQFMGSTSA CLAFIFPGAI LRDV+ IST RDK+VA++M+ LAV T
Sbjct: 361 YLAAIAIPNIWYLFQFMGSTSAVCLAFIFPGAIVLRDVHSISTTRDKIVAAVMIILAVAT 420

Query: 421 SIIAIATNINKALRNK 436
           S IA++TNI   +RNK
Sbjct: 421 STIALSTNIYSLVRNK 435

BLAST of Lsi04G010440 vs. TAIR10
Match: AT3G56200.1 (AT3G56200.1 Transmembrane amino acid transporter family protein)

HSP 1 Score: 474.6 bits (1220), Expect = 6.8e-134
Identity = 255/428 (59.58%), Postives = 332/428 (77.57%), Query Frame = 1

Query: 10  LLPEMKPAIKT---ASVSGAVFNVSTSIIGAGIMSIPFTLKVLGIIPAVVLIMLVAFMTD 69
           LLP+ +P+      +S SG VFNVSTSIIGAGIMS+P   KVLGI+PA ++I ++A+++ 
Sbjct: 9   LLPKQEPSSSENHGSSTSGIVFNVSTSIIGAGIMSMPAAFKVLGIVPAFLIITIIAWLST 68

Query: 70  LSVELLLRFTHSAESTTYAGVMKESFGPIGSVATQICVMITNLGCLIMYLIIIGDVLSGN 129
           +SV  L++ T + ESTTYAGVMKESFG  GS+A QI  M+   GC+I++ IIIGDVLSGN
Sbjct: 69  ISVGFLMKSTLAGESTTYAGVMKESFGKTGSIAVQIATMVATFGCMIIFSIIIGDVLSGN 128

Query: 130 KGGEEEVHLGVLQQWFGIQWWNSREFSILFTVVFILLPLVLYQRVDSLRFSSFVSVVLAV 189
           + G  E H+GVLQ+WFG  WWN+R F++LF   F+LLPLVL +RV+ L  SS VS +LAV
Sbjct: 129 ENGGPE-HIGVLQEWFGSYWWNTRIFALLFVYGFVLLPLVLRRRVERLAISSAVSFLLAV 188

Query: 190 VFVGISSVMAIMAMVEGKTKRTRLVPEVEDETSFFELFTAVPVIVTAFTFHFNVHPISFE 249
           +FV ISSV+AI A+V G+TK  RL PE+ +  SF++LFTA PVIVTAFTFHFNVHPI FE
Sbjct: 189 LFVVISSVLAISALVNGQTKNPRLFPELSNGGSFWQLFTASPVIVTAFTFHFNVHPIGFE 248

Query: 250 LRNSRDMMTAVRVALLLCAIIYFTIGIFGYMLFGESLMSDILTNFDQTFGSSTGALLNDI 309
           L++   ++ A +++++LCA IYF  G+FGY+LFG++ MSDIL NFDQ+ GSS G+LLNDI
Sbjct: 249 LKDPLQVIPATKISVILCAAIYFATGLFGYLLFGDATMSDILVNFDQSSGSSIGSLLNDI 308

Query: 310 VRLSYAFHLMLVYPLLNFSLRFNINELLFPYKPPLASDTTRFLTITMALLLFSYLAAIAF 369
           VRLSY  HLMLV+PLLNFSLR N++ELL+P KP L  DT RF+ +T+ALL+  +L+AIA 
Sbjct: 309 VRLSYVLHLMLVFPLLNFSLRANLDELLYPKKPSLEKDTKRFIGLTLALLICCFLSAIAV 368

Query: 370 PNIWSIFQFMGSTSAACLAFIFPGAIALRDVNGISTKRDKMVASIMVTLAVVTSIIAIAT 429
           P+IW  FQFMGST    +AFIFP AI LR+++G+ST R+K+VA+IM+ LAV TSIIAI+T
Sbjct: 369 PDIWYFFQFMGSTITVSIAFIFPAAIVLRNIHGVSTSREKIVAAIMLVLAVATSIIAIST 428

Query: 430 NINKALRN 435
           N+     N
Sbjct: 429 NLYSLAAN 435

BLAST of Lsi04G010440 vs. TAIR10
Match: AT2G40420.1 (AT2G40420.1 Transmembrane amino acid transporter family protein)

HSP 1 Score: 454.9 bits (1169), Expect = 5.6e-128
Identity = 248/433 (57.27%), Postives = 333/433 (76.91%), Query Frame = 1

Query: 1   MSPAPEHVPLLPEMKPAIKTA----SVSGAVFNVSTSIIGAGIMSIPFTLKVLGIIPAVV 60
           MSPA +  PLLP  +P+  ++    S +GAVFN+STSI+GAGIM+IP   KVLG+IP++ 
Sbjct: 1   MSPAIK-APLLPNQEPSSSSSENHGSFAGAVFNISTSIVGAGIMAIPAAFKVLGVIPSLS 60

Query: 61  LIMLVAFMTDLSVELLLRFTHSAESTTYAGVMKESFGPIGSVATQICVMITNLGCLIMYL 120
           +I+++A+++++S   L++ + + ESTTYAGVMKESFG  G+VA  +  M+   G +I++ 
Sbjct: 61  IIVIIAWLSNVSAGFLMKSSLAGESTTYAGVMKESFGKSGAVAVTVVTMVVTFGSMIIFS 120

Query: 121 IIIGDVLSGNKGGEEEVHLGVLQQWFGIQWWNSREFSILFTVVFILLPLVLYQRVDSLRF 180
           IIIGDV+SGN+  +  +HLG+LQ+WFG  WWN+R F +LF  VF+ LPLVL +RV+ L F
Sbjct: 121 IIIGDVISGNEK-DGIIHLGLLQEWFGSHWWNTRFFGLLFIFVFLFLPLVLCRRVERLAF 180

Query: 181 SSFVSVVLAVVFVGISSVMAIMAMVEGKTKRTRLVPEVEDE-TSFFELFTAVPVIVTAFT 240
           SS +S +LA++FV ISSV+AI+A+V+GKTK  RL PE+ D   SFF LFTA PVIVTAFT
Sbjct: 181 SSAISFLLALLFVVISSVLAIIALVQGKTKPPRLFPELNDGGLSFFSLFTASPVIVTAFT 240

Query: 241 FHFNVHPISFELRNSRDMMTAVRVALLLCAIIYFTIGIFGYMLFGESLMSDILTNFDQTF 300
           FHFNVHP++FEL++  ++++A R++++LCA IY   G+F Y+LFG+S M+D+L NFDQ+ 
Sbjct: 241 FHFNVHPVAFELKDPLNVLSATRISVILCATIYSATGLFCYLLFGDSTMTDVLMNFDQST 300

Query: 301 GSSTGALLNDIVRLSYAFHLMLVYPLLNFSLRFNINELLFPYKPPLASDTTRFLTITMAL 360
            SS G+LLNDIVRLSYA HLMLV+PLLNFSLR N++ELLFP K  L  D  RF  +T  L
Sbjct: 301 SSSVGSLLNDIVRLSYAIHLMLVFPLLNFSLRANLDELLFPMKLSLVEDNKRFFALTFPL 360

Query: 361 LLFSYLAAIAFPNIWSIFQFMGSTSAACLAFIFPGAIALRDVNGISTKRDKMVASIMVTL 420
           L+  +L AIA P+IW  FQF+GSTS   +AFIFP AI LR+VNG ST R+K+VAS+M+ L
Sbjct: 361 LISCFLGAIAIPDIWYFFQFLGSTSTVSIAFIFPAAIVLRNVNGFSTLREKIVASVMLVL 420

Query: 421 AVVTSIIAIATNI 429
           AV TSIIAI+TNI
Sbjct: 421 AVATSIIAISTNI 431

BLAST of Lsi04G010440 vs. TAIR10
Match: AT5G38820.1 (AT5G38820.1 Transmembrane amino acid transporter family protein)

HSP 1 Score: 358.2 bits (918), Expect = 7.1e-99
Identity = 194/407 (47.67%), Postives = 280/407 (68.80%), Query Frame = 1

Query: 21  ASVSGAVFNVSTSIIGAGIMSIPFTLKVLGIIPAVVLIMLVAFMTDLSVELLLRFTHSAE 80
           AS SGAVFN++T+IIGAGIM++P T+K+LG+IP + +I+L+AF+TD S+E LLRF++   
Sbjct: 35  ASFSGAVFNLATTIIGAGIMALPATMKILGLIPGITIIVLMAFLTDASIEFLLRFSNIGN 94

Query: 81  STTYAGVMKESFGPIGSVATQICVMITNLGCLIMYLIIIGDVLSG-NKGGEEEVHLGVLQ 140
             +Y GVM +SFG  G +  Q+ ++++N+G LI+Y+IIIGDVL+G N+ G    H G+L+
Sbjct: 95  QRSYGGVMDDSFGKCGRIMLQVSILVSNIGVLIVYMIIIGDVLAGKNEYGIH--HAGMLE 154

Query: 141 QWFGIQWWNSREFSILFTVVFILLPLVLYQRVDSLRFSSFVSVVLAVVFVGISSVMAIMA 200
            WFGI WWN R F +L T + +  PL  ++R+DSLRF+S +SV LAVVF+ I++ + I+ 
Sbjct: 155 GWFGISWWNRRTFVLLVTTLTVFAPLTCFKRIDSLRFTSAISVALAVVFLVITAGITIIK 214

Query: 201 MVEGKTKRTRLVPEVEDETSFFELFTAVPVIVTAFTFHFNVHPISFELRNSRDMMTAVRV 260
           +        RL+P V D +SF++LFT VPV+V A+  H+NVH I  EL +   +   VR 
Sbjct: 215 LFTDGLMMPRLLPNVTDLSSFWKLFTVVPVLVNAYICHYNVHSIQNELEDPSRIKPVVRS 274

Query: 261 ALLLCAIIYFTIGIFGYMLFGESLMSDILTNFDQTFGSSTGALLNDIVRLSYAFHLMLVY 320
           AL +C+ +Y    +FGY+LFG+  + D+L NFD   G   G++LND VR SYA HLMLV+
Sbjct: 275 ALAMCSSVYVMTSLFGYLLFGDGTLDDVLANFDTDLGIPFGSVLNDAVRFSYAAHLMLVF 334

Query: 321 PLLNFSLRFNINELLFPYKPPLASDTT--RFLTITMALLLFSYLAAIAFPNIWSIFQFMG 380
           P++ + LR NI+ L+FP  PPL S  +  RF +IT  L+   +L A   P+IW  FQF G
Sbjct: 335 PVVFYPLRINIDGLIFPTAPPLTSSESDLRFGSITAGLIAVIFLGANFIPSIWDAFQFTG 394

Query: 381 STSAACLAFIFPGAIALRDVNGISTKRDKMVASIMVTLAVVTSIIAI 425
           +T+A C+ FIFP A+ L+D +  +TKRDK +A  M+ LAV ++ IAI
Sbjct: 395 ATAAVCIGFIFPAAVILKDRHNQATKRDKTIAICMIVLAVFSNAIAI 439

BLAST of Lsi04G010440 vs. TAIR10
Match: AT3G30390.1 (AT3G30390.1 Transmembrane amino acid transporter family protein)

HSP 1 Score: 350.9 bits (899), Expect = 1.1e-96
Identity = 195/422 (46.21%), Postives = 282/422 (66.82%), Query Frame = 1

Query: 9   PLLPEMK------PAIKTASVSGAVFNVSTSIIGAGIMSIPFTLKVLGIIPAVVLIMLVA 68
           PLLPE             AS SGAVFN++T+IIGAGIM++P T+K+LG+   + +I+++A
Sbjct: 25  PLLPECHGDEVAHDEFNGASFSGAVFNLATTIIGAGIMALPATMKILGLGLGITMIVVMA 84

Query: 69  FMTDLSVELLLRFTHSAESTTYAGVMKESFGPIGSVATQICVMITNLGCLIMYLIIIGDV 128
           F+TD S+E LLRF+ + ++ +Y G+M  SFG  G +  Q+ V++ N+G LI+Y+IIIGDV
Sbjct: 85  FLTDASIEFLLRFSKAGKNRSYGGLMGGSFGNPGRILLQVAVLVNNIGVLIVYMIIIGDV 144

Query: 129 LSGNKGGEEEVHLGVLQQWFGIQWWNSREFSILFTVVFILLPLVLYQRVDSLRFSSFVSV 188
           L+G K  +   H GVL+ WFG  WWN R   +L T + +  PL  ++R+DSL+F+S +SV
Sbjct: 145 LAG-KTEDGIHHFGVLEGWFGHHWWNGRAAILLITTLGVFAPLACFKRIDSLKFTSALSV 204

Query: 189 VLAVVFVGISSVMAIMAMVEGKTKRTRLVPEVEDETSFFELFTAVPVIVTAFTFHFNVHP 248
            LAVVF+ I++ ++IM ++ G     RL+P+V D TSF+ LFT VPV+VTAF  H+NVH 
Sbjct: 205 ALAVVFLIITAGISIMKLISGGVAMPRLLPDVTDLTSFWNLFTVVPVLVTAFICHYNVHS 264

Query: 249 ISFELRNSRDMMTAVRVALLLCAIIYFTIGIFGYMLFGESLMSDILTNFDQTFGSSTGAL 308
           I  EL +   +   VR AL+LC+ +Y    IFG++LFG+  + D+L NFD   G   G++
Sbjct: 265 IQNELEDPSQIRPVVRSALMLCSSVYIMTSIFGFLLFGDDTLDDVLANFDTDLGIPFGSI 324

Query: 309 LNDIVRLSYAFHLMLVYPLLNFSLRFNINELLFPYKPPLASDTTRFLTITMALLLFSYLA 368
           LND VR+SYA HLMLV+P++ + LR NI+ LLFP    L++   RF  +T  L+   +L 
Sbjct: 325 LNDAVRVSYALHLMLVFPIVFYPLRINIDGLLFPSARSLSTSNVRFGCLTAGLISVIFLG 384

Query: 369 AIAFPNIWSIFQFMGSTSAACLAFIFPGAIALRDVNGISTKRDKMVASIMVTLAVVTSII 425
           A   P+IW  FQF G+T+A CL FIFP +I L+D +  +T RD  +A  M+ LAV+++ I
Sbjct: 385 ANFIPSIWDAFQFTGATAAVCLGFIFPASIILKDRHDKATNRDTTLAIFMIVLAVLSNAI 444

BLAST of Lsi04G010440 vs. TAIR10
Match: AT1G80510.1 (AT1G80510.1 Transmembrane amino acid transporter family protein)

HSP 1 Score: 290.8 bits (743), Expect = 1.4e-78
Identity = 174/419 (41.53%), Postives = 262/419 (62.53%), Query Frame = 1

Query: 15  KPAIKTASVSGAVFNVSTSIIGAGIMSIPFTLKVLGIIPAVVLIMLVAFMTDLSVELLLR 74
           K + + + + GAVFN++TSIIGAGIM++P T+KVLG++   VLI+L+A ++++SVELL+R
Sbjct: 69  KSSNQGSGIYGAVFNLTTSIIGAGIMALPATMKVLGLVLGFVLIILMALLSEISVELLVR 128

Query: 75  FTHSAESTTYAGVMKESFGPIGSVATQICVMITNLGCLIMYLIIIGDVLSGNKGGEEEVH 134
           F+   +S +Y  V++ + G    V ++IC+++ N G L++YLII+GDV+SG+       H
Sbjct: 129 FSVLYKSKSYGEVVQFALGKTARVLSEICIIVNNGGVLVVYLIIMGDVMSGSLH-----H 188

Query: 135 LGVLQQWFGIQWWNSREFSILFTVVFILLPLVLYQRVDSLRFSSFVSVVLAVVFVGISSV 194
           +GVL QW G  +W+ R+  IL  +V  L PL    ++DSL  +S  SV LAVVFV +  V
Sbjct: 189 IGVLDQWLGNGFWDHRKVLILIVMVIFLAPLCALNKIDSLSVTSAASVALAVVFVVVCFV 248

Query: 195 MAIMAMVEGKTKRTRLVPEVEDETSFFELFTAVPVIVTAFTFHFNVHPISFEL--RNSRD 254
           +A + ++EG     RL P+   + +  +L   +P++  A+  HFNV PI  EL  R+   
Sbjct: 249 VATIKLIEGTIDPPRLSPDFGSKQAILDLLVVIPIMSNAYVCHFNVQPIYNELEGRSPHK 308

Query: 255 MMTAVRVALLLCAIIYFTIGIFGYMLFGESLMSDILTNFDQTFGSSTGALLNDIVRLSYA 314
           M    R+   +C ++Y +  + GY+LFG+   SDILTNFDQ  G    + +N IVR+ Y 
Sbjct: 309 MNRVGRITTAICVVVYASTAVSGYLLFGKDTESDILTNFDQDLGIRFSSAVNYIVRIGYI 368

Query: 315 FHLMLVYPLLNFSLRFNINELLFPYKPPLASDTTRFLTITMALLLFSYLAAIAFPNIWSI 374
            HL+LV+P+++FSLR  +N LLF   PPL+    R L +T+ LL   Y+ +   PNIW+ 
Sbjct: 369 LHLVLVFPVIHFSLRETVNTLLFEGSPPLSESKKRSLGLTVVLLALIYIGSTMIPNIWTA 428

Query: 375 FQFMGSTSAACLAFIFPGAIALR---DVNGISTKRDKMVASIMVTLAVVTSIIAIATNI 429
           F+F G+TSA  L F FP  IALR     N +S   ++ V+ +M+ LAVV SI+    NI
Sbjct: 429 FKFTGATSAVSLGFTFPALIALRLGKQSNSLSFV-ERSVSWLMLILAVVVSIVGTIGNI 481

BLAST of Lsi04G010440 vs. NCBI nr
Match: gi|449457433|ref|XP_004146453.1| (PREDICTED: sodium-coupled neutral amino acid transporter 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 754.2 bits (1946), Expect = 1.3e-214
Identity = 412/439 (93.85%), Postives = 421/439 (95.90%), Query Frame = 1

Query: 1   MSPAPEHVPLLPEMKPAIKTASVSGAVFNVSTSIIGAGIMSIPFTLKVLGIIPAVVLIML 60
           MSP PEHVPLLPEMKP IKTASVSGAVFNVSTSIIGAGIMSIPFTLKVLGIIPAV LIML
Sbjct: 1   MSPPPEHVPLLPEMKPPIKTASVSGAVFNVSTSIIGAGIMSIPFTLKVLGIIPAVALIML 60

Query: 61  VAFMTDLSVELLLRFTHSAESTTYAGVMKESFGPIGSVATQICVMITNLGCLIMYLIIIG 120
           VAFMTDLSVELLLRFTHS+ STTYAGVMKESFG IGSVATQICVMITNLGCLIMYLIIIG
Sbjct: 61  VAFMTDLSVELLLRFTHSSNSTTYAGVMKESFGLIGSVATQICVMITNLGCLIMYLIIIG 120

Query: 121 DVLSGNKGGE--EEVHLGVLQQWFGIQWWNSREFSILFTVVFILLPLVLYQRVDSLRFSS 180
           DVLSGNKGG   EEVH GVLQQWFG  WWNSREFSILFTVVFILLPLVLYQRVDSLRFSS
Sbjct: 121 DVLSGNKGGGGGEEVHFGVLQQWFGNHWWNSREFSILFTVVFILLPLVLYQRVDSLRFSS 180

Query: 181 FVSVVLAVVFVGISSVMAIMAMVEGKTKRTRLVPEVEDETSFFELFTAVPVIVTAFTFHF 240
           FVSVVLAVVFVGISSVMA+MA+VEGKTKRTRLVPEVEDETSFFELFTAVPVIVTAFTFHF
Sbjct: 181 FVSVVLAVVFVGISSVMAVMAIVEGKTKRTRLVPEVEDETSFFELFTAVPVIVTAFTFHF 240

Query: 241 NVHPISFELRNSRDMMTAVRVALLLCAIIYFTIGIFGYMLFGESLMSDILTNFDQTFGSS 300
           NVHPISFELRNSR+MMTAVRVAL+LCAIIYF IGIFGY+LFG+SLMSDILTNFDQTFGSS
Sbjct: 241 NVHPISFELRNSRNMMTAVRVALILCAIIYFAIGIFGYLLFGDSLMSDILTNFDQTFGSS 300

Query: 301 TGA-LLNDIVRLSYAFHLMLVYPLLNFSLRFNINELLFPYKPPLASDTTRFLTITMALLL 360
           TGA LLNDIVRLSYAFHLMLVYPLLNFSLRFNINELLFP +PPLASDTTRF TITMALLL
Sbjct: 301 TGANLLNDIVRLSYAFHLMLVYPLLNFSLRFNINELLFPNRPPLASDTTRFFTITMALLL 360

Query: 361 FSYLAAIAFPNIWSIFQFMGSTSAACLAFIFPGAIALRDVNGISTKRDKMVASIMVTLAV 420
           FSYLAAIAFPNIWSIFQFMGSTSAACLAFIFPGAIALRDVNGISTK DKMVAS MV+LAV
Sbjct: 361 FSYLAAIAFPNIWSIFQFMGSTSAACLAFIFPGAIALRDVNGISTKTDKMVASTMVSLAV 420

Query: 421 VTSIIAIATNINKALRNKF 437
           VTSIIAIATNINKAL NKF
Sbjct: 421 VTSIIAIATNINKALNNKF 439

BLAST of Lsi04G010440 vs. NCBI nr
Match: gi|659114097|ref|XP_008456903.1| (PREDICTED: sodium-coupled neutral amino acid transporter 4-like [Cucumis melo])

HSP 1 Score: 740.0 bits (1909), Expect = 2.5e-210
Identity = 405/439 (92.26%), Postives = 417/439 (94.99%), Query Frame = 1

Query: 1   MSPAPEHVPLLPEMKPAIKTASVSGAVFNVSTSIIGAGIMSIPFTLKVLGIIPAVVLIML 60
           MSP PEHVPLLPEMKP IK ASVSGAVFNVSTSIIGAGIMSIPFTLKVLGIIPAVVLIML
Sbjct: 1   MSPPPEHVPLLPEMKPPIKPASVSGAVFNVSTSIIGAGIMSIPFTLKVLGIIPAVVLIML 60

Query: 61  VAFMTDLSVELLLRFTHSAESTTYAGVMKESFGPIGSVATQICVMITNLGCLIMYLIIIG 120
           VAFMTDLSVELLLRFT S  STTYAGVMKESFG IGSVATQICVMITNLGCLIMYLIIIG
Sbjct: 61  VAFMTDLSVELLLRFTRSGNSTTYAGVMKESFGSIGSVATQICVMITNLGCLIMYLIIIG 120

Query: 121 DVLSGNKGGEE--EVHLGVLQQWFGIQWWNSREFSILFTVVFILLPLVLYQRVDSLRFSS 180
           DVLSGNKGG    EVHLGVLQQWFG  WWNSREFSILFTVVFIL PLVLY+RVDSLRFSS
Sbjct: 121 DVLSGNKGGGGGGEVHLGVLQQWFGNHWWNSREFSILFTVVFILFPLVLYERVDSLRFSS 180

Query: 181 FVSVVLAVVFVGISSVMAIMAMVEGKTKRTRLVPEVEDETSFFELFTAVPVIVTAFTFHF 240
           FVSV+LAVVFVGIS+VMA+MA+VEGKTKRTRLVPEVEDETSFFELFTAVPVIVTAFTFHF
Sbjct: 181 FVSVILAVVFVGISTVMAVMAIVEGKTKRTRLVPEVEDETSFFELFTAVPVIVTAFTFHF 240

Query: 241 NVHPISFELRNSRDMMTAVRVALLLCAIIYFTIGIFGYMLFGESLMSDILTNFDQTFGSS 300
           NVHPISFELRNSR+MM AVRVAL+LCAIIYFTIGIFGY+LFG+SLMSDILTNFDQTFGSS
Sbjct: 241 NVHPISFELRNSRNMMMAVRVALILCAIIYFTIGIFGYLLFGDSLMSDILTNFDQTFGSS 300

Query: 301 TGA-LLNDIVRLSYAFHLMLVYPLLNFSLRFNINELLFPYKPPLASDTTRFLTITMALLL 360
           TGA LLNDIVRLSYAFHLMLVYPLLNFSLR NINELLFP +P LASDTTRF TITMALLL
Sbjct: 301 TGANLLNDIVRLSYAFHLMLVYPLLNFSLRSNINELLFPNRPTLASDTTRFFTITMALLL 360

Query: 361 FSYLAAIAFPNIWSIFQFMGSTSAACLAFIFPGAIALRDVNGISTKRDKMVASIMVTLAV 420
           FSYLAAIAFPNIWSIFQFMGSTSAACLAFIFPGAIALRDVNGISTK+DKMVAS MV+LAV
Sbjct: 361 FSYLAAIAFPNIWSIFQFMGSTSAACLAFIFPGAIALRDVNGISTKKDKMVASTMVSLAV 420

Query: 421 VTSIIAIATNINKALRNKF 437
           VTSIIAIATNINKAL NKF
Sbjct: 421 VTSIIAIATNINKALNNKF 439

BLAST of Lsi04G010440 vs. NCBI nr
Match: gi|449457431|ref|XP_004146452.1| (PREDICTED: probable sodium-coupled neutral amino acid transporter 6 [Cucumis sativus])

HSP 1 Score: 644.0 bits (1660), Expect = 1.8e-181
Identity = 348/435 (80.00%), Postives = 389/435 (89.43%), Query Frame = 1

Query: 1   MSPAPEHVPLLPEMKPAIKTASVSGAVFNVSTSIIGAGIMSIPFTLKVLGIIPAVVLIML 60
           MSPAPE VPLLP  KP++KTASVSGAVFNVSTSIIGAGIMSIPF LKVLGIIPA+VLI+ 
Sbjct: 1   MSPAPEQVPLLPAAKPSMKTASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVF 60

Query: 61  VAFMTDLSVELLLRFTHSAESTTYAGVMKESFGPIGSVATQICVMITNLGCLIMYLIIIG 120
           VAF+TD+SVELLLRFTHS +STTYAGVMKESFGPIGS+ATQ+C+MITNLGCLIMY IIIG
Sbjct: 61  VAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIGSIATQVCIMITNLGCLIMYQIIIG 120

Query: 121 DVLSGNKGGEEEVHLGVLQQWFGIQWWNSREFSILFTVVFILLPLVLYQRVDSLRFSSFV 180
           DVLSGNK G + VHLGVLQ+WFG  WWN+REFSILFT+VFIL PLVL++RVDSLRFSSF+
Sbjct: 121 DVLSGNKEGGK-VHLGVLQEWFGHHWWNTREFSILFTLVFILFPLVLFRRVDSLRFSSFI 180

Query: 181 SVVLAVVFVGISSVMAIMAMVEGKTKRTRLVPEVEDETSFFELFTAVPVIVTAFTFHFNV 240
           SVVLAVVF+GISSVMAIMA+VEGKTK TRLVPE+++ETSFF+LFTAVPV+VTAF FHFNV
Sbjct: 181 SVVLAVVFIGISSVMAIMAIVEGKTKSTRLVPELDEETSFFDLFTAVPVLVTAFCFHFNV 240

Query: 241 HPISFELRNSRDMMTAVRVALLLCAIIYFTIGIFGYMLFGESLMSDILTNFDQTFGSSTG 300
           HPIS EL    +M TAVR+ALLLCAI YFTIGIFGY+LFG+SLMSDIL NFD++ G +TG
Sbjct: 241 HPISSELHKPSNMTTAVRIALLLCAIFYFTIGIFGYLLFGDSLMSDILMNFDES-GDTTG 300

Query: 301 ALLNDIVRLSYAFHLMLVYPLLNFSLRFNINELLFPYKPPLASDTTRFLTITMALLLFSY 360
           ALLND+VRLSYA HLMLV+PLLNF LR NI EL FP K    +D  RFL IT+ALL+FSY
Sbjct: 301 ALLNDVVRLSYAVHLMLVFPLLNFPLRSNIIELFFPKKASSGTDQRRFLGITLALLVFSY 360

Query: 361 LAAIAFPNIWSIFQFMGSTSAACLAFIFPGAIALRDVNGISTKRDKMVASIMVTLAVVTS 420
           LAAIAFPNIW IFQFMGSTSAACLAFIFPGAIALRDVNGI+TK+DK+VA  MV LAV TS
Sbjct: 361 LAAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILAVGTS 420

Query: 421 IIAIATNINKALRNK 436
           I+AI+ NIN AL NK
Sbjct: 421 IVAISININNALENK 433

BLAST of Lsi04G010440 vs. NCBI nr
Match: gi|659114106|ref|XP_008456905.1| (PREDICTED: sodium-coupled neutral amino acid transporter 4-like [Cucumis melo])

HSP 1 Score: 642.1 bits (1655), Expect = 7.0e-181
Identity = 346/435 (79.54%), Postives = 389/435 (89.43%), Query Frame = 1

Query: 1   MSPAPEHVPLLPEMKPAIKTASVSGAVFNVSTSIIGAGIMSIPFTLKVLGIIPAVVLIML 60
           MSPAPE VPLLP  KP+IKTASVSGAVFNVSTSIIGAGIMSIPF LKVLGIIPA+VLI+ 
Sbjct: 1   MSPAPEQVPLLPPAKPSIKTASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVF 60

Query: 61  VAFMTDLSVELLLRFTHSAESTTYAGVMKESFGPIGSVATQICVMITNLGCLIMYLIIIG 120
           VAF+TD+SVELLLRFTHS +STTYAGVMKESFGPIGS+ TQ+C+MITNLGCLIMY IIIG
Sbjct: 61  VAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIGSITTQVCIMITNLGCLIMYQIIIG 120

Query: 121 DVLSGNKGGEEEVHLGVLQQWFGIQWWNSREFSILFTVVFILLPLVLYQRVDSLRFSSFV 180
           DVLSGNK G + VH+GVLQ+WFG  WWN+REFSILFT+VFIL PLVL++RVDSLRFSSF+
Sbjct: 121 DVLSGNKEGGK-VHIGVLQEWFGHHWWNTREFSILFTLVFILFPLVLFRRVDSLRFSSFI 180

Query: 181 SVVLAVVFVGISSVMAIMAMVEGKTKRTRLVPEVEDETSFFELFTAVPVIVTAFTFHFNV 240
           SVVLAVVF+GISSVMAIMA+VEGKTK TRLVPE+++ETSFF LFTAVPV+VT+F FHFNV
Sbjct: 181 SVVLAVVFIGISSVMAIMAIVEGKTKSTRLVPEIDEETSFFNLFTAVPVLVTSFCFHFNV 240

Query: 241 HPISFELRNSRDMMTAVRVALLLCAIIYFTIGIFGYMLFGESLMSDILTNFDQTFGSSTG 300
           HPIS EL    +M+TAVR+ALLLCAI YFTIGIFGY+LFG+SLMSDIL NFD++ G +TG
Sbjct: 241 HPISSELHKPSNMITAVRIALLLCAIFYFTIGIFGYLLFGDSLMSDILMNFDES-GDTTG 300

Query: 301 ALLNDIVRLSYAFHLMLVYPLLNFSLRFNINELLFPYKPPLASDTTRFLTITMALLLFSY 360
           ALLND+VRLSYA HLMLV+PLLNF LR NI EL FP K  L +D  RFL IT ALL+FSY
Sbjct: 301 ALLNDVVRLSYAVHLMLVFPLLNFPLRSNIMELFFPKKASLGTDQRRFLGITTALLVFSY 360

Query: 361 LAAIAFPNIWSIFQFMGSTSAACLAFIFPGAIALRDVNGISTKRDKMVASIMVTLAVVTS 420
           LAAIAFPNIW IFQFMGSTSAACLAFIFPGAIALRDVNGI+TK+DK+VA  MV LA+ TS
Sbjct: 361 LAAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILALGTS 420

Query: 421 IIAIATNINKALRNK 436
           IIAI+ NIN AL++K
Sbjct: 421 IIAISININNALKSK 433

BLAST of Lsi04G010440 vs. NCBI nr
Match: gi|703063797|ref|XP_010087043.1| (hypothetical protein L484_012287 [Morus notabilis])

HSP 1 Score: 586.3 bits (1510), Expect = 4.5e-164
Identity = 315/437 (72.08%), Postives = 374/437 (85.58%), Query Frame = 1

Query: 1   MSPA-PEHVPLLPEM-KPAIKTASVSGAVFNVSTSIIGAGIMSIPFTLKVLGIIPAVVLI 60
           MSPA   HVPLLP   K   K AS+SGAVFNV+TSIIGAGIMSIP TLKVLG+IPA VLI
Sbjct: 1   MSPAIGVHVPLLPNHDKQQAKPASISGAVFNVATSIIGAGIMSIPATLKVLGVIPAFVLI 60

Query: 61  MLVAFMTDLSVELLLRFTHSAESTTYAGVMKESFGPIGSVATQICVMITNLGCLIMYLII 120
           ++V  + D+SVE L+RFTHS ++TTY GVMKESFG +GSVATQICVMITN GCLIMYLII
Sbjct: 61  VIVGLLVDISVEFLMRFTHSGDATTYGGVMKESFGQVGSVATQICVMITNFGCLIMYLII 120

Query: 121 IGDVLSGNKGGEEEVHLGVLQQWFGIQWWNSREFSILFTVVFILLPLVLYQRVDSLRFSS 180
           IGDVLSG+K  E  VHLGVLQ+WFGI WWNSREFSILFTVVFI+LPL+L +RV+SLRFSS
Sbjct: 121 IGDVLSGSKP-EGSVHLGVLQEWFGIHWWNSREFSILFTVVFIMLPLLLLRRVESLRFSS 180

Query: 181 FVSVVLAVVFVGISSVMAIMAMVEGKTKRTRLVPEVEDETSFFELFTAVPVIVTAFTFHF 240
            VSV+LAV+FV I SVMAI+A+V+GKTK  RL+PE++ +TSFF+LFTAVPVIVTAFTFHF
Sbjct: 181 AVSVLLAVIFVAICSVMAIIALVQGKTKNPRLIPELDQQTSFFDLFTAVPVIVTAFTFHF 240

Query: 241 NVHPISFELRNSRDMMTAVRVALLLCAIIYFTIGIFGYMLFGESLMSDILTNFDQTFGSS 300
           NVHPI FE+    DM+ AVR+AL+LCA IYF +G+FGY+LFG+S+M+DIL NFD++  ++
Sbjct: 241 NVHPIGFEMGKPSDMVKAVRIALVLCAAIYFAVGLFGYLLFGDSIMTDILVNFDKSSDTA 300

Query: 301 TGALLNDIVRLSYAFHLMLVYPLLNFSLRFNINELLFPYKPPLASDTTRFLTITMALLLF 360
           TG++LND VRLSYA HLMLV+PLLNFSLR NI+E LFP K  L +DT RF+ IT+ LL+F
Sbjct: 301 TGSVLNDAVRLSYALHLMLVFPLLNFSLRANIDEFLFPEKILLVNDTKRFVIITLVLLVF 360

Query: 361 SYLAAIAFPNIWSIFQFMGSTSAACLAFIFPGAIALRDVNGISTKRDKMVASIMVTLAVV 420
           SYLAAIAFPNIW IFQF+GSTSA CLAFIFPGAIALRDVNGIST++D+++A+IM+  AVV
Sbjct: 361 SYLAAIAFPNIWYIFQFLGSTSAVCLAFIFPGAIALRDVNGISTRKDRILATIMIVSAVV 420

Query: 421 TSIIAIATNINKALRNK 436
           TSIIAI+ NI  +  NK
Sbjct: 421 TSIIAISINIYNSFGNK 436