Lsi04G009420 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi04G009420
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionTransmembrane amino acid transporter family protein
Locationchr04 : 9754349 .. 9759350 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGGCCGCCCTCCTGGATTTGAATAGCATATTCGAAGAGCGGGGGGCAGAGAGCGAGTCGCTCGTAGCTGCACTCCGGACGGTGGAACAACACTCACTTATACCACCATTCAACCATGGTGATGAACAAAATATGGAGGTCGCCGTACCGGAAACTGCCCATCAGATCAGCACTGGTTCGTCTTTCTTCGTTTTTGTCTTTCCCTTTTTGATTTTCTGCAATTTGTTTCCATAGTGTCCTTCTGCTGTAGAGTTATGAAATGAGAAATTGAAGAAGTTGTTATTGTGTTCTTTTTCAATGCTTAGATTCATGGTTTCAAGTGGGCTCTGTCCTTACCACTGGTATCAACAGTGCTTATGTACTGGAATACTCGGCGACTATAATGGTTCCTCTTGGTTGGATAGCTGGAGTTGTTGGTTTCATTGCAGCCACCGCCATTTCATTATATGCAAATATTCTTATTGCCCACCTCCATGAATACGGTGGGAAGAGGCATATCAGATACAGAGATTTAGCAGCCTTTATATACGGTATGTGAATATACCAAAATGTCAATGATTAAGTGCAACCTATACTATCACATACCACCAATTCTAATTTTTTTTTTAAAAAAAGAATCTGCCATGTGCCAGAGTTTCATTGGGCTGCACAGGTGCATAGCAAAATAATGGAGTGATTGTTTTGGCTCCTTCTTCTTGTTTTGGCTCCTTCTTTATTCCTTCTTGATGGGTTTGTTCTTGCAGGTAGAAGAGCATATTCTGTTACATGGGGATTACAATATGTTAATCTTTTTATGATTAACGTGGGATACATCATCATAGCTGGTCAAGCCTTGAAGGTGAGATCAATATAAGTATACTGATCATACTTTCAAACTAATGAAATTGCTAATTCTTACTCTTAGTAATGGTTTGAACAAGTCAAATAGCTTGAAAAGTATTAACGAAAGTTTATTGAAGTATTGGCGTTGGTAACTTGTCTTAGTGCAAATTAGAAGACTAGAACACTTAAAAGAAAGAAAAAAAAAAAAATGATAAAACAAGTTGATTTGAAATAAAGTAGGAAAATAGAAGTTGTTTTTATCCTTCGCAGGAATTTTGCACTCATTAATAATGTCCGCTTTGAAACGAACGGCTGTTATATTTTATTTTATCAATTTAACTTTTTCTTTTTAAGAAAATGTCAATAATCTTGTTAATTTGATAGGCCATGATTTTAGGGAAAATAGATTAAGAAAAGAAAGATTTAAGAAATTAACCTACAATATAACTAATATTTCAAAAATGTCCTAAAATTTAATAATACTTCATAGTTAGATATTAGCAACATTTTTAGACATTTTTAACTGTCTCTCATATTTTAATTTCATGATATTTTTAAATAAAAAAATATATCATTTATGAATTTATATGAAGATTTTATTTTTTGAAATATCATTAATTATATTATCTCTCATCAATAAATTTTTGAAATATTTATCTCTTTCCTTATTAACATCATTTTCGAATTTTAATTTTGTTTTTGTTATTTATTAATTTCTCTTGATATATATTGAATTCAAGATATTTCAAATTAATGTTACCTTTATTTTAGGAGATTTCGTTTTTTTTTCATATTCCTACTTGAAAATCGATCCCCCTCTCCCATGAGATCTCAAGTATATGTCAAATATACTGGTAATATACAGAAAAATGTAAATGTACATCACATAAACGATGGTTTATTTTTGGGAAATTATTGTAAACCAAAAAAATATCAAACTATTTACAAATATAGAAAAATTTTACTATCTATCACGGTCTATCGCTAATAGATAGTAAAATTTTTCTATATTTATAAATAGTTTTGCTCATTTTTCGATCTATATTTGAAAACAACCCTATATTTTTTGGCATGTTTACAAATATAATAGAAAACGTAAATATACATCGCATATATTATTCGGTATATAAGATGATATATTGTACAAATTTTCTTGTTCATGCATTTGTCAAATATAACTTGGTTTGTTTCTATAAAATTCAAATACATAGTTTGTATATGTCGAATACACCTTGTTTTCTCATTTAAAGATATTTGACGATTCTTGCTTTCATTTATGAAGGCAATAATTCTCGAATTTTTGGAGAAACTTTCCTTACTTGTAGGCTTTGAAAAGAAAACCATTTGAGAAATTCATAACTACAAGAAACTTCTCTTACAGGCTTCTTTTGGTGTTATATATATATATATATAACTTAATGGTAAGGAGGGTTGAAAAAGTAAATTGAGTCAGACTTTGTGTAGAAAAATAGCTATTAGTCTATTTATGTAAAAAATAGAAAAGTTTAGACAATTGTGTCATTAGGTCTATTTATCTAAGCTATATGTGTAGAAAGCCCTTAAAAAAATTAAGATTTATTTTAATTTATCGTGATAAATTTGTCGGAATTAATAAATGGTATGTGACTCTTCGTTTAGGGATCCTTTTAATTTTATGAATTGCACTCTTTCATGAATGGATATGAATTTTCAAAATTTAATTAACTTGAAGACTTTTCAATTAGCCACGAGTTAGTCTATAAATATATGTCTCTTCTTTAAATTGGTTTTCACCTTTTCCTAAGCGCTTTACTCCCATGAAACTTTTTCCTTTTTATAAATTTGTTGAAGTTCGGTTTATTCCACAAAATTGAGTTCGTTGGATCTTATGGACGAGATGATACTTCCATTAGAAAAGTAATCTAATTCTATCTTAAGAGATAATTACTCTTGAAACCCATGCACTAAAGTTAGTAAAATTGTCTTAAGGAGACAACGTGAATTCATTGAGTTCAGGTTCTCTCCTATATACAAGGTTTTGTATTTTCTATTTTTATGTGATATTATTCATATAACAAAATTTACCTTCCTTTCTTTAATATGGGATCAAAGATGGTCTAAATCTTTTGCCAAACCTATACCAAAATGTCCACTATAGTACCATTAACGGATGAGAAAAGGAAAAGAAAGTTAATACCTAGAGAAAAGTTTGGCAAGATAATAATGGAAGCCAGATTAACAAAAGAAAGTAAAGCATGAAAACAAAAGGTCAATTGGTATATCATGTCCTCTTAATGGAGAAATTATGAAATTGGAACGCTAATATAGTATGTTCATTGTAGAATTTTGTATTGTACTGTGACCAATGAGTTATTACTTGTCTGTTCTGTAGGCTGTTTATGTCCTCTACAATGATGACCATGCTATGAAGCTTCCCTATTTTATAGTCATTGCTGGCTTTGTTTGTGCCTTGTTTGCAATATCAATACCCCATTTATCAGCTCTAAGAATTTGGCTGGGATTCTCAATTGTCTTCAGTTTCATATACATTGTTGTTGCATTTGTGCTGTCTGTTCAGGATGGTAAGTTTCATTTTCCATTGATAAAGATTTCCTTAGTTTAAAAGTTCCCATTAATGGGTTTTGCTGTTCCAGGAGTTAATGCACCTGCAAGAGATTATAACATTCCAGGATCGTCGACTAGCAAAATTTTTACCACGATTGGAGCATCTGCTAATTTGGTTTTTGTCTTCAATACTGGAATGCTTCCAGAGATTCAGGTATGGCAAGAACTCTAACATGATATACCATCCATGTTTAAACTTTACATTTATGTATGTGTCGAACTTATCTTCTTCAAACCTTCATTGCTCACGCTACAACAATTTTGGCTTTAAATGTTAATTTTGAATTGACATCGTATACCTATAATGACAGATTACACACTGTCGTCGTGTCACATGTCATTCTAGAGTATGATGACGGATTAATCTTTGAGAATCACCATCAAAACTTCTAATATTATATATAGTACAATCACCGTTATCTATGATGACGGTTGCTAACTGACATCAAATCCTGTGTCATCATATGTATACAATGTCGGGTCGATATGATGTCAGTTGGGAACTAACATCATCTTCATGTCATCATACACTATAATGAGGGTTTGGTATGATGTTAGTTTATAATCGACATTGTATGCATAATGGACGACATTGTATGCAAAATCGACATTATAGCATAAATGGAACTCAAATCTTGCATAAATATTTTTTTTTACAATAATAATAATTCTCAGCCCCTATTTCAATTAATTCATTCACACAAATGTTTAGTGAAATAGAGATAAACTTCTAAAACACAATCTATCATTTCATTTCAATATCGATCCATTCACACAATTGAGTCTTTCAATACAATGCTTTCAAAAAAGCTACACAATATGTTTCAAATTTATACAAAATGCTTAAGATTTACCAAAATTTCAAAAAACCTCTGAAATAGCTGGACAATTGTTGTTACAGGCCACATTGAGGCAGCCTGTAGTGAAGAACATGCTAAAGGCTCTTTACTTCCAGTTCATCATCGGAGGTTTGCCCATGTATGCAGTCACTTTTGCCGGATACTGGGCATATGGATCTTCCACCTCCACTTATTTACTCAACAGCGTAAAGGGTCCAGTTTGGGTGAAGGCAATGGCTAGCATATCCGCTTTCCTTCAAACAGTCATCTCTCTGCACGTAAAAAGACTATCCCTCCCTTTCCTCGCTAAATTCTCAATCCATTTTAACTCAATGATTGTTTGTTGTTTTGATTAGATATTTGCTAGCCCGATGTACGAGTATTTGGACACGAAGTACGGGATTGTAGGAAGCGCGCTGAATATAAAAAACTTGTCATTCAGAATAGGTGTGAGAGGAGGATACATGGGCATAACCACACTGCTATCAGCTATGCTGCCTTTCATTGGAGACTTTATGAGCCTAACAGGAGCAATCACCACACTCCCACTTACATTTATCCTTGCAAACCACATGTACCTTGTGGCAGAGAAGCCTAAACTAAGTTCTTTACACCAGCTTTGGCATTGGTTCAATGTTTGTTTCTTTGGCTGTATGTCTTTAGCAGCAGGAGTTGCAGCATTGAGGCTCATAGCTATAGACTCCAAAAACTATAATTTATTTGCTGATCTATGA

mRNA sequence

ATGAAGGCCGCCCTCCTGGATTTGAATAGCATATTCGAAGAGCGGGGGGCAGAGAGCGAGTCGCTCGTAGCTGCACTCCGGACGGTGGAACAACACTCACTTATACCACCATTCAACCATGGTGATGAACAAAATATGGAGGTCGCCGTACCGGAAACTGCCCATCAGATCAGCACTGATTCATGGTTTCAAGTGGGCTCTGTCCTTACCACTGGTATCAACAGTGCTTATGTACTGGAATACTCGGCGACTATAATGGTTCCTCTTGGTTGGATAGCTGGAGTTGTTGGTTTCATTGCAGCCACCGCCATTTCATTATATGCAAATATTCTTATTGCCCACCTCCATGAATACGGTGGGAAGAGGCATATCAGATACAGAGATTTAGCAGCCTTTATATACGGTAGAAGAGCATATTCTGTTACATGGGGATTACAATATGTTAATCTTTTTATGATTAACGTGGGATACATCATCATAGCTGGTCAAGCCTTGAAGGCTGTTTATGTCCTCTACAATGATGACCATGCTATGAAGCTTCCCTATTTTATAGTCATTGCTGGCTTTGTTTGTGCCTTGTTTGCAATATCAATACCCCATTTATCAGCTCTAAGAATTTGGCTGGGATTCTCAATTGTCTTCAGTTTCATATACATTGTTGTTGCATTTGTGCTGTCTGTTCAGGATGGAGTTAATGCACCTGCAAGAGATTATAACATTCCAGGATCGTCGACTAGCAAAATTTTTACCACGATTGGAGCATCTGCTAATTTGGTTTTTGTCTTCAATACTGGAATGCTTCCAGAGATTCAGTTCATCATCGGAGGTTTGCCCATGTATGCAGTCACTTTTGCCGGATACTGGGCATATGGATCTTCCACCTCCACTTATTTACTCAACAGCGTAAAGGGTCCAGTTTGGGTGAAGGCAATGGCTAGCATATCCGCTTTCCTTCAAACAGTCATCTCTCTGCACATATTTGCTAGCCCGATGTACGAGTATTTGGACACGAAGTACGGGATTGTAGGAAGCGCGCTGAATATAAAAAACTTGTCATTCAGAATAGGTGTGAGAGGAGGATACATGGGCATAACCACACTGCTATCAGCTATGCTGCCTTTCATTGGAGACTTTATGAGCCTAACAGGAGCAATCACCACACTCCCACTTACATTTATCCTTGCAAACCACATGTACCTTGTGGCAGAGAAGCCTAAACTAAGTTCTTTACACCAGCTTTGGCATTGGTTCAATGTTTGTTTCTTTGGCTGTATGTCTTTAGCAGCAGGAGTTGCAGCATTGAGGCTCATAGCTATAGACTCCAAAAACTATAATTTATTTGCTGATCTATGA

Coding sequence (CDS)

ATGAAGGCCGCCCTCCTGGATTTGAATAGCATATTCGAAGAGCGGGGGGCAGAGAGCGAGTCGCTCGTAGCTGCACTCCGGACGGTGGAACAACACTCACTTATACCACCATTCAACCATGGTGATGAACAAAATATGGAGGTCGCCGTACCGGAAACTGCCCATCAGATCAGCACTGATTCATGGTTTCAAGTGGGCTCTGTCCTTACCACTGGTATCAACAGTGCTTATGTACTGGAATACTCGGCGACTATAATGGTTCCTCTTGGTTGGATAGCTGGAGTTGTTGGTTTCATTGCAGCCACCGCCATTTCATTATATGCAAATATTCTTATTGCCCACCTCCATGAATACGGTGGGAAGAGGCATATCAGATACAGAGATTTAGCAGCCTTTATATACGGTAGAAGAGCATATTCTGTTACATGGGGATTACAATATGTTAATCTTTTTATGATTAACGTGGGATACATCATCATAGCTGGTCAAGCCTTGAAGGCTGTTTATGTCCTCTACAATGATGACCATGCTATGAAGCTTCCCTATTTTATAGTCATTGCTGGCTTTGTTTGTGCCTTGTTTGCAATATCAATACCCCATTTATCAGCTCTAAGAATTTGGCTGGGATTCTCAATTGTCTTCAGTTTCATATACATTGTTGTTGCATTTGTGCTGTCTGTTCAGGATGGAGTTAATGCACCTGCAAGAGATTATAACATTCCAGGATCGTCGACTAGCAAAATTTTTACCACGATTGGAGCATCTGCTAATTTGGTTTTTGTCTTCAATACTGGAATGCTTCCAGAGATTCAGTTCATCATCGGAGGTTTGCCCATGTATGCAGTCACTTTTGCCGGATACTGGGCATATGGATCTTCCACCTCCACTTATTTACTCAACAGCGTAAAGGGTCCAGTTTGGGTGAAGGCAATGGCTAGCATATCCGCTTTCCTTCAAACAGTCATCTCTCTGCACATATTTGCTAGCCCGATGTACGAGTATTTGGACACGAAGTACGGGATTGTAGGAAGCGCGCTGAATATAAAAAACTTGTCATTCAGAATAGGTGTGAGAGGAGGATACATGGGCATAACCACACTGCTATCAGCTATGCTGCCTTTCATTGGAGACTTTATGAGCCTAACAGGAGCAATCACCACACTCCCACTTACATTTATCCTTGCAAACCACATGTACCTTGTGGCAGAGAAGCCTAAACTAAGTTCTTTACACCAGCTTTGGCATTGGTTCAATGTTTGTTTCTTTGGCTGTATGTCTTTAGCAGCAGGAGTTGCAGCATTGAGGCTCATAGCTATAGACTCCAAAAACTATAATTTATTTGCTGATCTATGA

Protein sequence

MKAALLDLNSIFEERGAESESLVAALRTVEQHSLIPPFNHGDEQNMEVAVPETAHQISTDSWFQVGSVLTTGINSAYVLEYSATIMVPLGWIAGVVGFIAATAISLYANILIAHLHEYGGKRHIRYRDLAAFIYGRRAYSVTWGLQYVNLFMINVGYIIIAGQALKAVYVLYNDDHAMKLPYFIVIAGFVCALFAISIPHLSALRIWLGFSIVFSFIYIVVAFVLSVQDGVNAPARDYNIPGSSTSKIFTTIGASANLVFVFNTGMLPEIQFIIGGLPMYAVTFAGYWAYGSSTSTYLLNSVKGPVWVKAMASISAFLQTVISLHIFASPMYEYLDTKYGIVGSALNIKNLSFRIGVRGGYMGITTLLSAMLPFIGDFMSLTGAITTLPLTFILANHMYLVAEKPKLSSLHQLWHWFNVCFFGCMSLAAGVAALRLIAIDSKNYNLFADL
BLAST of Lsi04G009420 vs. Swiss-Prot
Match: PROT2_ARATH (Proline transporter 2 OS=Arabidopsis thaliana GN=PROT2 PE=1 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 4.4e-170
Identity = 304/425 (71.53%), Postives = 347/425 (81.65%), Query Frame = 1

Query: 44  QNMEVAVPETAHQISTDSWFQVGSVLTTGINSAYVLEYSATIMVPLGWIAGVVGFIAATA 103
           +  ++ VPETAHQIS+DSWFQV  VLTTGINSAYVL YS T+MVPLGWI GVVG I ATA
Sbjct: 15  EQFDLEVPETAHQISSDSWFQVAFVLTTGINSAYVLGYSGTVMVPLGWIGGVVGLILATA 74

Query: 104 ISLYANILIAHLHEYGGKRHIRYRDLAAFIYGRRAYSVTWGLQYVNLFMINVGYIIIAGQ 163
           ISLYAN LIA LHE+GGKRHIRYRDLA FIYG++ Y VTWGLQYVNLFMIN G+II+AG 
Sbjct: 75  ISLYANTLIAKLHEFGGKRHIRYRDLAGFIYGKKMYRVTWGLQYVNLFMINCGFIILAGS 134

Query: 164 ALKAVYVLYNDDHAMKLPYFIVIAGFVCALFAISIPHLSALRIWLGFSIVFSFIYIVVAF 223
           ALKAVYVL+ DD  MKLP+FI IAG VCA+FAI IPHLSAL IWLG S + S IYI+VA 
Sbjct: 135 ALKAVYVLFRDDSLMKLPHFIAIAGVVCAIFAIGIPHLSALGIWLGVSTILSIIYIIVAI 194

Query: 224 VLSVQDGVNAPARDYNIPGSSTSKIFTTIGASANLVFVFNTGMLPEI------------- 283
           VLS +DGVN P RDYNI GSS +K+FT  GA+ANLVF FNTGMLPEI             
Sbjct: 195 VLSAKDGVNKPERDYNIQGSSINKLFTITGAAANLVFAFNTGMLPEIQATVKQPVVKNMM 254

Query: 284 -----QFIIGGLPMYAVTFAGYWAYGSSTSTYLLNSVKGPVWVKAMASISAFLQTVISLH 343
                QF +G LPMYAVTF GYWAYGSSTSTYLLNSV GPVWVKA+A+ISAFLQ+VISLH
Sbjct: 255 KALYFQFTVGVLPMYAVTFIGYWAYGSSTSTYLLNSVSGPVWVKALANISAFLQSVISLH 314

Query: 344 IFASPMYEYLDTKYGIVGSALNIKNLSFRIGVRGGYMGITTLLSAMLPFIGDFMSLTGAI 403
           IFASP YEY+DTKYG+ GS L +KNL FR   RG Y+ ++TLLSA+LPF+GDFMSLTGAI
Sbjct: 315 IFASPTYEYMDTKYGVKGSPLAMKNLLFRTVARGSYIAVSTLLSALLPFLGDFMSLTGAI 374

Query: 404 TTLPLTFILANHMYLVAEKPKLSSLHQLWHWFNVCFFGCMSLAAGVAALRLIAIDSKNYN 451
           +T PLTFILANHMYLVA   +LS + +LWHW NVCFFG MSLAA +AA+RLI++DSKN++
Sbjct: 375 STFPLTFILANHMYLVAMNDELSLVQKLWHWLNVCFFGLMSLAAAIAAVRLISVDSKNFH 434

BLAST of Lsi04G009420 vs. Swiss-Prot
Match: PROT1_ARATH (Proline transporter 1 OS=Arabidopsis thaliana GN=PROT1 PE=1 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 2.0e-167
Identity = 299/431 (69.37%), Postives = 349/431 (80.97%), Query Frame = 1

Query: 39  NHGDEQNM-EVAVPETAHQISTDSWFQVGSVLTTGINSAYVLEYSATIMVPLGWIAGVVG 98
           N GD  ++ ++ +P+TAHQIS+DSWFQV  VLTTGINSAYVL YS TIMVPLGWI GVVG
Sbjct: 12  NVGDGDDVVDIEIPDTAHQISSDSWFQVAFVLTTGINSAYVLGYSGTIMVPLGWIGGVVG 71

Query: 99  FIAATAISLYANILIAHLHEYGGKRHIRYRDLAAFIYGRRAYSVTWGLQYVNLFMINVGY 158
            + ATAISLYAN LIA LHE+GG+RHIRYRDLA FIYGR+AY +TWGLQYVNLFMIN G+
Sbjct: 72  LLIATAISLYANTLIAKLHEFGGRRHIRYRDLAGFIYGRKAYHLTWGLQYVNLFMINCGF 131

Query: 159 IIIAGQALKAVYVLYNDDHAMKLPYFIVIAGFVCALFAISIPHLSALRIWLGFSIVFSFI 218
           II+AG ALKAVYVL+ DDH MKLP+FI IAG +CA+FAI IPHLSAL +WLG S   S I
Sbjct: 132 IILAGSALKAVYVLFRDDHTMKLPHFIAIAGLICAIFAIGIPHLSALGVWLGVSTFLSLI 191

Query: 219 YIVVAFVLSVQDGVNAPARDYNIPGSSTSKIFTTIGASANLVFVFNTGMLPEI------- 278
           YIVVA VLSV+DGV  P+RDY I GSS SK+FT  GA+ANLVF FNTGMLPEI       
Sbjct: 192 YIVVAIVLSVRDGVKTPSRDYEIQGSSLSKLFTITGAAANLVFAFNTGMLPEIQATVRQP 251

Query: 279 -----------QFIIGGLPMYAVTFAGYWAYGSSTSTYLLNSVKGPVWVKAMASISAFLQ 338
                      QF  G LPMYAVTF GYWAYGSSTSTYLLNSV GP+WVKA+A++SA LQ
Sbjct: 252 VVKNMMKALYFQFTAGVLPMYAVTFIGYWAYGSSTSTYLLNSVNGPLWVKALANVSAILQ 311

Query: 339 TVISLHIFASPMYEYLDTKYGIVGSALNIKNLSFRIGVRGGYMGITTLLSAMLPFIGDFM 398
           +VISLHIFASP YEY+DTKYGI G+   IKNL FRI  RGGY+ ++TL+SA+LPF+GDFM
Sbjct: 312 SVISLHIFASPTYEYMDTKYGIKGNPFAIKNLLFRIMARGGYIAVSTLISALLPFLGDFM 371

Query: 399 SLTGAITTLPLTFILANHMYLVAEKPKLSSLHQLWHWFNVCFFGCMSLAAGVAALRLIAI 451
           SLTGA++T PLTFILANHMY  A+  KL+++ +LWHW NV FF  MS+AA +AA+RLIA+
Sbjct: 372 SLTGAVSTFPLTFILANHMYYKAKNNKLNAMQKLWHWLNVVFFSLMSVAAAIAAVRLIAV 431

BLAST of Lsi04G009420 vs. Swiss-Prot
Match: PROT3_ARATH (Proline transporter 3 OS=Arabidopsis thaliana GN=PROT3 PE=1 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 5.2e-163
Identity = 292/425 (68.71%), Postives = 343/425 (80.71%), Query Frame = 1

Query: 44  QNMEVAVPETAHQISTDSWFQVGSVLTTGINSAYVLEYSATIMVPLGWIAGVVGFIAATA 103
           + +++ +P+TAHQIS+DSWFQ   VLTT INSAYVL YS T+MVPLGWI GVVG I ATA
Sbjct: 12  EGVDIEIPDTAHQISSDSWFQAAFVLTTSINSAYVLGYSGTVMVPLGWIGGVVGLILATA 71

Query: 104 ISLYANILIAHLHEYGGKRHIRYRDLAAFIYGRRAYSVTWGLQYVNLFMINVGYIIIAGQ 163
           ISLYAN L+A LHE+GGKRHIRYRDLA FIYGR+AY +TW LQYVNLFMIN G+II+AG 
Sbjct: 72  ISLYANTLVAKLHEFGGKRHIRYRDLAGFIYGRKAYCLTWVLQYVNLFMINCGFIILAGS 131

Query: 164 ALKAVYVLYNDDHAMKLPYFIVIAGFVCALFAISIPHLSALRIWLGFSIVFSFIYIVVAF 223
           ALKAVYVL+ DDHAMKLP+FI IAG +CA+FAI IPHLSAL IWL  S + S IYIVVA 
Sbjct: 132 ALKAVYVLFRDDHAMKLPHFIAIAGLICAVFAIGIPHLSALGIWLAVSTILSLIYIVVAI 191

Query: 224 VLSVQDGVNAPARDYNIPGSSTSKIFTTIGASANLVFVFNTGMLPEI------------- 283
           VLSV+DGV AP+RDY I GS  SK+FT  GA+A LVFVFNTGMLPEI             
Sbjct: 192 VLSVKDGVKAPSRDYEIQGSPLSKLFTITGAAATLVFVFNTGMLPEIQATVKQPVVKNMM 251

Query: 284 -----QFIIGGLPMYAVTFAGYWAYGSSTSTYLLNSVKGPVWVKAMASISAFLQTVISLH 343
                QF +G LPM+AV F GYWAYGSSTS YLLN+V GP+WVKA+A+ISA LQ+VISLH
Sbjct: 252 KALYFQFTVGVLPMFAVVFIGYWAYGSSTSPYLLNNVNGPLWVKALANISAILQSVISLH 311

Query: 344 IFASPMYEYLDTKYGIVGSALNIKNLSFRIGVRGGYMGITTLLSAMLPFIGDFMSLTGAI 403
           IFASP YEY+DTK+GI G+ L +KNL FRI  RGGY+ ++TLLSA+LPF+GDFMSLTGA+
Sbjct: 312 IFASPTYEYMDTKFGIKGNPLALKNLLFRIMARGGYIAVSTLLSALLPFLGDFMSLTGAV 371

Query: 404 TTLPLTFILANHMYLVAEKPKLSSLHQLWHWFNVCFFGCMSLAAGVAALRLIAIDSKNYN 451
           +T PLTFILANHMY  A+  KL++L +L HW NV FF  MS+AA +AALRLIA+DSKN++
Sbjct: 372 STFPLTFILANHMYYKAKNNKLNTLQKLCHWLNVVFFSLMSVAAAIAALRLIALDSKNFH 431

BLAST of Lsi04G009420 vs. Swiss-Prot
Match: PROT2_ORYSJ (Probable proline transporter 2 OS=Oryza sativa subsp. japonica GN=LOC_Os07g01090 PE=2 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 1.8e-160
Identity = 285/418 (68.18%), Postives = 335/418 (80.14%), Query Frame = 1

Query: 52  ETAHQISTDSWFQVGSVLTTGINSAYVLEYSATIMVPLGWIAGVVGFIAATAISLYANIL 111
           +TAHQIS D W+QVG VLTTG+NSAYVL YS ++MVPLGWI G  G I A AISLYAN L
Sbjct: 17  DTAHQISADPWYQVGFVLTTGVNSAYVLGYSGSVMVPLGWIGGTCGLILAAAISLYANAL 76

Query: 112 IAHLHEYGGKRHIRYRDLAAFIYGRRAYSVTWGLQYVNLFMINVGYIIIAGQALKAVYVL 171
           +A LHE GGKRHIRYRDLA  IYGR+ YS+TW LQYVNLFMIN G+II+AGQALKA YVL
Sbjct: 77  LARLHEIGGKRHIRYRDLAGHIYGRKMYSLTWALQYVNLFMINTGFIILAGQALKATYVL 136

Query: 172 YNDDHAMKLPYFIVIAGFVCALFAISIPHLSALRIWLGFSIVFSFIYIVVAFVLSVQDGV 231
           + DD  +KLPY I ++GFVCALFA  IP+LSALRIWLGFS  FS IYI +AFVLS++DG+
Sbjct: 137 FRDDGVLKLPYCIALSGFVCALFAFGIPYLSALRIWLGFSTFFSLIYITIAFVLSLRDGI 196

Query: 232 NAPARDYNIPGSSTSKIFTTIGASANLVFVFNTGMLPEI------------------QFI 291
             PA+DY IPGS +++IFTTIGA ANLVF +NTGMLPEI                  QF 
Sbjct: 197 TTPAKDYTIPGSHSARIFTTIGAVANLVFAYNTGMLPEIQATIRPPVVKNMEKALWFQFT 256

Query: 292 IGGLPMYAVTFAGYWAYGSSTSTYLLNSVKGPVWVKAMASISAFLQTVISLHIFASPMYE 351
           +G LP+YAVTF GYWAYGSSTS+YLLNSVKGPVWVKAMA++SAFLQTVI+LHIFASPMYE
Sbjct: 257 VGSLPLYAVTFMGYWAYGSSTSSYLLNSVKGPVWVKAMANLSAFLQTVIALHIFASPMYE 316

Query: 352 YLDTKYGI-VGSALNIKNLSFRIGVRGGYMGITTLLSAMLPFIGDFMSLTGAITTLPLTF 411
           +LDTKYG   G    I N+ FR+GVRGGY+ + TL++AMLPF+GDFMSLTGA++T PLTF
Sbjct: 317 FLDTKYGSGHGGPFAIHNVMFRVGVRGGYLTVNTLVAAMLPFLGDFMSLTGALSTFPLTF 376

Query: 412 ILANHMYLVAEKPKLSSLHQLWHWFNVCFFGCMSLAAGVAALRLIAIDSKNYNLFADL 451
           +LANHMYL+ ++ KLS+L   WHW NV  F  +S+AA VAALRLI +DS+ Y+LFADL
Sbjct: 377 VLANHMYLMVKRHKLSTLQISWHWLNVAGFSLLSIAAAVAALRLIMVDSRTYHLFADL 434

BLAST of Lsi04G009420 vs. Swiss-Prot
Match: PROT1_ORYSJ (Proline transporter 1 OS=Oryza sativa subsp. japonica GN=PROT1 PE=2 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 2.2e-158
Identity = 287/471 (60.93%), Postives = 354/471 (75.16%), Query Frame = 1

Query: 2   KAALLDLNSIFEERGAESESLVAALRTVEQHSLIPPFN--HGDEQNMEVAVPE-TAHQIS 61
           +AAL   ++     GA+ E         E+   +P  +    D+++  V V E TAHQIS
Sbjct: 12  RAALFHSSAPSSSLGADGE---------EERETVPLLSCKMADDKSDTVQVSEDTAHQIS 71

Query: 62  TDSWFQVGSVLTTGINSAYVLEYSATIMVPLGWIAGVVGFIAATAISLYANILIAHLHEY 121
            D W+QVG +LTTG+NSAYVL YSA+IMVPLGWI G  G I A AIS+YAN L+AHLHE 
Sbjct: 72  IDPWYQVGFILTTGVNSAYVLGYSASIMVPLGWIGGTCGLILAAAISMYANALLAHLHEV 131

Query: 122 GGKRHIRYRDLAAFIYGRRAYSVTWGLQYVNLFMINVGYIIIAGQALKAVYVLYNDDHAM 181
           GGKRHIRYRDLA  IYGR+ YS+TW LQYVNLFMIN G II+AGQALKA+YVL+ DD  +
Sbjct: 132 GGKRHIRYRDLAGHIYGRKMYSLTWALQYVNLFMINTGLIILAGQALKAIYVLFRDDGVL 191

Query: 182 KLPYFIVIAGFVCALFAISIPHLSALRIWLGFSIVFSFIYIVVAFVLSVQDGVNAPARDY 241
           KLPY I ++GFVCALFA  IP+LSALRIWLG S VFS IYI++AFV+S++DG+  PA+DY
Sbjct: 192 KLPYCIALSGFVCALFAFGIPYLSALRIWLGLSTVFSLIYIMIAFVMSLRDGITTPAKDY 251

Query: 242 NIPGSSTSKIFTTIGASANLVFVFNTGMLPEI------------------QFIIGGLPMY 301
            IPGS + +IFTTIGA ANLVF +NTGMLPEI                  QF +G LP+Y
Sbjct: 252 TIPGSHSDRIFTTIGAVANLVFAYNTGMLPEIQATIRPPVVKNMEKALWFQFTVGSLPLY 311

Query: 302 AVTFAGYWAYGSSTSTYLLNSVKGPVWVKAMASISAFLQTVISLHIFASPMYEYLDTKYG 361
           AVTF GYWAYGSSTS+YLLNSVKGP+W+K +A++SAFLQTVI+LHIFASPMYE+LDT++G
Sbjct: 312 AVTFMGYWAYGSSTSSYLLNSVKGPIWIKTVANLSAFLQTVIALHIFASPMYEFLDTRFG 371

Query: 362 I-VGSALNIKNLSFRIGVRGGYMGITTLLSAMLPFIGDFMSLTGAITTLPLTFILANHMY 421
              G    I N+ FR+GVRGGY+ + TL++AMLPF+GDFMSLTGA++T PLTF+LANHMY
Sbjct: 372 SGHGGPFAIHNIMFRVGVRGGYLTVNTLVAAMLPFLGDFMSLTGALSTFPLTFVLANHMY 431

Query: 422 LVAEKPKLSSLHQLWHWFNVCFFGCMSLAAGVAALRLIAIDSKNYNLFADL 451
           L  ++ K+S   + WHW NV  F C+S+AA VAA+RLI +D   Y+LFAD+
Sbjct: 432 LTVKQNKMSIFRKCWHWLNVVGFSCLSVAAAVAAVRLITVDYSTYHLFADM 473

BLAST of Lsi04G009420 vs. TrEMBL
Match: A0A0A0KQE7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G197090 PE=4 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 9.1e-191
Identity = 345/432 (79.86%), Postives = 375/432 (86.81%), Query Frame = 1

Query: 37  PFNHGDEQNMEVAVPETAHQISTDSWFQVGSVLTTGINSAYVLEYSATIMVPLGWIAGVV 96
           P N  DE+ MEVA P+TAHQIS+DSWFQ G VLTTGINSAYVL YS TIMVPLGWI GVV
Sbjct: 3   PINDVDEEKMEVAAPQTAHQISSDSWFQAGFVLTTGINSAYVLGYSGTIMVPLGWIGGVV 62

Query: 97  GFIAATAISLYANILIAHLHEYGGKRHIRYRDLAAFIYGRRAYSVTWGLQYVNLFMINVG 156
           G IAATAISLYAN LIA LHE+GGKRHIRYRDLA FIYGR AYS+TWGLQY NLFMINVG
Sbjct: 63  GLIAATAISLYANCLIAKLHEFGGKRHIRYRDLAGFIYGRTAYSLTWGLQYANLFMINVG 122

Query: 157 YIIIAGQALKAVYVLYNDDHAMKLPYFIVIAGFVCALFAISIPHLSALRIWLGFSIVFSF 216
           YII+AGQALKA+YVL++DDH MKLPYFI IAG VCALFAISIPHLSAL+IWLGFS VFS 
Sbjct: 123 YIILAGQALKALYVLFSDDHVMKLPYFIAIAGVVCALFAISIPHLSALKIWLGFSTVFSL 182

Query: 217 IYIVVAFVLSVQDGVNAPARDYNIPGSSTSKIFTTIGASANLVFVFNTGMLPEI------ 276
           +YI+VAF+LS++DGV  PA DY++PGSSTSKIFTTIGASANLVF FNTGMLPEI      
Sbjct: 183 VYIIVAFILSLRDGVKTPA-DYSLPGSSTSKIFTTIGASANLVFAFNTGMLPEIQATVRQ 242

Query: 277 ------------QFIIGGLPMYAVTFAGYWAYGSSTSTYLLNSVKGPVWVKAMASISAFL 336
                       QF  G LPMYAVTF GYWAYGSSTSTYLLNSV GP+W+KA A+ISAFL
Sbjct: 243 PVVKNMLKALYFQFTAGVLPMYAVTFIGYWAYGSSTSTYLLNSVNGPIWIKAAANISAFL 302

Query: 337 QTVISLHIFASPMYEYLDTKYGIVGSALNIKNLSFRIGVRGGYMGITTLLSAMLPFIGDF 396
           QTVI+LHIFASPMYEYLDTKYGI GSALNIKNLSFRI VRGGY+ ITTL+SAMLPF+GDF
Sbjct: 303 QTVIALHIFASPMYEYLDTKYGITGSALNIKNLSFRIVVRGGYLAITTLISAMLPFLGDF 362

Query: 397 MSLTGAITTLPLTFILANHMYLVAEKPKLSSLHQLWHWFNVCFFGCMSLAAGVAALRLIA 451
           MSLTGAI+TLPLTFILANHMYLVA+K KL+SL +LWHW NVCFFGCMSLAA VAA+RLIA
Sbjct: 363 MSLTGAISTLPLTFILANHMYLVAKKTKLNSLQRLWHWLNVCFFGCMSLAAAVAAVRLIA 422

BLAST of Lsi04G009420 vs. TrEMBL
Match: B9HT23_POPTR (Proline transporter 2 family protein OS=Populus trichocarpa GN=POPTR_0010s20180g PE=4 SV=1)

HSP 1 Score: 647.5 bits (1669), Expect = 1.2e-182
Identity = 329/427 (77.05%), Postives = 369/427 (86.42%), Query Frame = 1

Query: 42  DEQNMEVAVPETAHQISTDSWFQVGSVLTTGINSAYVLEYSATIMVPLGWIAGVVGFIAA 101
           DE N  V +PETAHQIS+DSWFQVG VLTTGINSAYVL YS TIMVPLGWI GVVG I A
Sbjct: 19  DEDNSAVEIPETAHQISSDSWFQVGFVLTTGINSAYVLGYSGTIMVPLGWIPGVVGLIIA 78

Query: 102 TAISLYANILIAHLHEYGGKRHIRYRDLAAFIYGRRAYSVTWGLQYVNLFMINVGYIIIA 161
           TAISLYAN LIA LHE+GG+RHIRYRDLA FIYGR+AYS+TWGLQYVNLFMIN GYII+A
Sbjct: 79  TAISLYANSLIAELHEFGGRRHIRYRDLAGFIYGRKAYSLTWGLQYVNLFMINTGYIILA 138

Query: 162 GQALKAVYVLYNDDHAMKLPYFIVIAGFVCALFAISIPHLSALRIWLGFSIVFSFIYIVV 221
           G ALKAVYVL++DDH MKLPYFI I+GFVCALFA+SIPHLSALR+WLG S VFS IYIVV
Sbjct: 139 GSALKAVYVLFSDDHVMKLPYFIAISGFVCALFAMSIPHLSALRLWLGVSTVFSLIYIVV 198

Query: 222 AFVLSVQDGVNAPARDYNIPGSSTSKIFTTIGASANLVFVFNTGMLPEI----------- 281
           AFVLSV+DG+ APARDY+IPG++ SKIFTTIGASANLVF FNTGMLPEI           
Sbjct: 199 AFVLSVKDGIEAPARDYSIPGTTRSKIFTTIGASANLVFAFNTGMLPEIQATIKQPVVSN 258

Query: 282 -------QFIIGGLPMYAVTFAGYWAYGSSTSTYLLNSVKGPVWVKAMASISAFLQTVIS 341
                  QF  G LPMYAVTF GYWAYGSSTS+YLL+SV GPVWVKA+A+ISAFLQTVI+
Sbjct: 259 MMKSLYFQFSAGVLPMYAVTFIGYWAYGSSTSSYLLSSVNGPVWVKALANISAFLQTVIA 318

Query: 342 LHIFASPMYEYLDTKYGIVGSALNIKNLSFRIGVRGGYMGITTLLSAMLPFIGDFMSLTG 401
           LHIFASPMYEYLDTKYGI+GS  +I+NLSFR+GVRGGY+ I TL++A+LPF+GDFMSLTG
Sbjct: 319 LHIFASPMYEYLDTKYGIIGSPFSIRNLSFRVGVRGGYLTINTLVAALLPFLGDFMSLTG 378

Query: 402 AITTLPLTFILANHMYLVAEKPKLSSLHQLWHWFNVCFFGCMSLAAGVAALRLIAIDSKN 451
           AI+T PLTFILANHMYL A+K KL+SL +LWHWFNV FFG MS+AA V+ALRLIA+DSK 
Sbjct: 379 AISTFPLTFILANHMYLKAKKNKLTSLQKLWHWFNVYFFGLMSIAAAVSALRLIAVDSKT 438

BLAST of Lsi04G009420 vs. TrEMBL
Match: B9HHJ8_POPTR (Proline transporter 2 family protein OS=Populus trichocarpa GN=POPTR_0008s06340g PE=4 SV=2)

HSP 1 Score: 633.6 bits (1633), Expect = 1.8e-178
Identity = 321/427 (75.18%), Postives = 362/427 (84.78%), Query Frame = 1

Query: 42  DEQNMEVAVPETAHQISTDSWFQVGSVLTTGINSAYVLEYSATIMVPLGWIAGVVGFIAA 101
           DE+   V +PETAHQIS+DSWFQVG VLTTGINSAYVL YS TIMVPLGWI GV+G I A
Sbjct: 20  DEEKSTVVIPETAHQISSDSWFQVGFVLTTGINSAYVLGYSGTIMVPLGWIPGVIGLIIA 79

Query: 102 TAISLYANILIAHLHEYGGKRHIRYRDLAAFIYGRRAYSVTWGLQYVNLFMINVGYIIIA 161
           TAISLYAN L+A LHE+GG+RHIRYRDLA FIYGR+AYS+TW LQYVNLFMIN GYII+A
Sbjct: 80  TAISLYANSLVAKLHEFGGRRHIRYRDLAGFIYGRKAYSITWALQYVNLFMINTGYIILA 139

Query: 162 GQALKAVYVLYNDDHAMKLPYFIVIAGFVCALFAISIPHLSALRIWLGFSIVFSFIYIVV 221
           G ALKA YVL++DD  MKLPYFI I+GFVCALF ISIPHLSALR+WLG S V S IYIVV
Sbjct: 140 GSALKAFYVLFSDDQVMKLPYFIAISGFVCALFGISIPHLSALRLWLGVSTVLSLIYIVV 199

Query: 222 AFVLSVQDGVNAPARDYNIPGSSTSKIFTTIGASANLVFVFNTGMLPEI----------- 281
           AFVLSV+DG+ AP+RDYNIPG++TSKIFTTIGASANLVF FNTGMLPEI           
Sbjct: 200 AFVLSVKDGIEAPSRDYNIPGTTTSKIFTTIGASANLVFAFNTGMLPEIQATIKEPVVSN 259

Query: 282 -------QFIIGGLPMYAVTFAGYWAYGSSTSTYLLNSVKGPVWVKAMASISAFLQTVIS 341
                  QF  G LPMYAVTF GYWAYGSSTSTYLL+SV GPVWVK +A+ISAFLQTVI+
Sbjct: 260 MMKALYFQFTAGVLPMYAVTFIGYWAYGSSTSTYLLSSVNGPVWVKGLANISAFLQTVIA 319

Query: 342 LHIFASPMYEYLDTKYGIVGSALNIKNLSFRIGVRGGYMGITTLLSAMLPFIGDFMSLTG 401
           LHIFASPMYEYLDTK+G+ GS L I+NLSFRIGVRGGY+ I TL++A+LPF+GDFMSLTG
Sbjct: 320 LHIFASPMYEYLDTKFGVKGSPLAIRNLSFRIGVRGGYLTINTLVAALLPFLGDFMSLTG 379

Query: 402 AITTLPLTFILANHMYLVAEKPKLSSLHQLWHWFNVCFFGCMSLAAGVAALRLIAIDSKN 451
           AI+T PLTFILANHMYL A+  KL+SL +LWHW NVCFFG MS+AA V+ALRLIA+DSK 
Sbjct: 380 AISTFPLTFILANHMYLKAKHNKLTSLQKLWHWLNVCFFGLMSIAALVSALRLIAVDSKT 439

BLAST of Lsi04G009420 vs. TrEMBL
Match: W9R1P4_9ROSA (Lysine histidine transporter 1 OS=Morus notabilis GN=L484_001878 PE=3 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 3.0e-178
Identity = 317/427 (74.24%), Postives = 361/427 (84.54%), Query Frame = 1

Query: 42  DEQNMEVAVPETAHQISTDSWFQVGSVLTTGINSAYVLEYSATIMVPLGWIAGVVGFIAA 101
           DE ++ + VPETAHQIS+DSWFQVG VLTTGINSAYVL YS T+MVPLGW+ GVVG IAA
Sbjct: 441 DEDHLAIEVPETAHQISSDSWFQVGFVLTTGINSAYVLGYSGTVMVPLGWVFGVVGLIAA 500

Query: 102 TAISLYANILIAHLHEYGGKRHIRYRDLAAFIYGRRAYSVTWGLQYVNLFMINVGYIIIA 161
           TAISLYAN L+A LHE+GGKRHIRYRDLA FIYG++AYS+TWGLQY NLFMIN G+II+A
Sbjct: 501 TAISLYANTLVAKLHEFGGKRHIRYRDLAGFIYGKKAYSLTWGLQYANLFMINTGFIILA 560

Query: 162 GQALKAVYVLYNDDHAMKLPYFIVIAGFVCALFAISIPHLSALRIWLGFSIVFSFIYIVV 221
           GQALKAVYVL+ DDH +KLPY I IAG VCALFAI IPHLSALRIWLGFS  FS +YIV 
Sbjct: 561 GQALKAVYVLFRDDHLLKLPYCIAIAGVVCALFAIGIPHLSALRIWLGFSTFFSLVYIVT 620

Query: 222 AFVLSVQDGVNAPARDYNIPGSSTSKIFTTIGASANLVFVFNTGMLPEI----------- 281
           AFVLS++DG+ AP RDYNIPG+ T KIFTTIGA+ANLVF FNTGMLPEI           
Sbjct: 621 AFVLSLRDGIKAPERDYNIPGTRTDKIFTTIGAAANLVFAFNTGMLPEIQATVRKPVVGN 680

Query: 282 -------QFIIGGLPMYAVTFAGYWAYGSSTSTYLLNSVKGPVWVKAMASISAFLQTVIS 341
                  QF +G LP+YAV F GYWAYGSSTSTYLLNSV GPVW+KA A++SAFLQTVI+
Sbjct: 681 MLKALYFQFTVGVLPLYAVAFMGYWAYGSSTSTYLLNSVSGPVWIKAAANLSAFLQTVIA 740

Query: 342 LHIFASPMYEYLDTKYGIVGSALNIKNLSFRIGVRGGYMGITTLLSAMLPFIGDFMSLTG 401
           LHIFASPMYEY+DTK+GI GSAL + NL+FRI VRGGY+ I TL++A+LPF+GDFMSLTG
Sbjct: 741 LHIFASPMYEYVDTKFGIKGSALALHNLAFRIMVRGGYLAINTLVAALLPFLGDFMSLTG 800

Query: 402 AITTLPLTFILANHMYLVAEKPKLSSLHQLWHWFNVCFFGCMSLAAGVAALRLIAIDSKN 451
           AI+T PLTFILANHMYLVA+K KL+SL +LWHW NVCFFG MS+ A +AALRLIA+DSK 
Sbjct: 801 AISTFPLTFILANHMYLVAKKNKLTSLQKLWHWLNVCFFGFMSITAAIAALRLIAVDSKT 860

BLAST of Lsi04G009420 vs. TrEMBL
Match: B9SWN5_RICCO (Amino acid transporter, putative OS=Ricinus communis GN=RCOM_0498260 PE=4 SV=1)

HSP 1 Score: 628.2 bits (1619), Expect = 7.5e-177
Identity = 314/427 (73.54%), Postives = 361/427 (84.54%), Query Frame = 1

Query: 42  DEQNMEVAVPETAHQISTDSWFQVGSVLTTGINSAYVLEYSATIMVPLGWIAGVVGFIAA 101
           DE+ + V +PETAHQIS+DSWFQVG VLTTGINSAYVL YS  IMVPLGWIAGVVG + A
Sbjct: 17  DEEKLAVQIPETAHQISSDSWFQVGFVLTTGINSAYVLGYSGIIMVPLGWIAGVVGLVLA 76

Query: 102 TAISLYANILIAHLHEYGGKRHIRYRDLAAFIYGRRAYSVTWGLQYVNLFMINVGYIIIA 161
            AISLYAN L+A LHEYGGKRHIRYRDLA +IYG++AYS+TWGLQYVNLFMIN GYII+A
Sbjct: 77  AAISLYANSLVAKLHEYGGKRHIRYRDLAGYIYGKKAYSITWGLQYVNLFMINTGYIILA 136

Query: 162 GQALKAVYVLYNDDHAMKLPYFIVIAGFVCALFAISIPHLSALRIWLGFSIVFSFIYIVV 221
           GQALKAVYVLY DDH MKLPYFI IAGFVC LFAI+ PHLSALRIWLG S V S IYI+V
Sbjct: 137 GQALKAVYVLYRDDHEMKLPYFIAIAGFVCTLFAIATPHLSALRIWLGVSTVLSLIYIIV 196

Query: 222 AFVLSVQDGVNAPARDYNIPGSSTSKIFTTIGASANLVFVFNTGMLPEI----------- 281
           A VLS++DGVNAP RDY+IPGSST+KIFT+IG  A+LVF FNTGMLPEI           
Sbjct: 197 AIVLSIKDGVNAPPRDYSIPGSSTAKIFTSIGGGASLVFAFNTGMLPEIQATIRQPVVSN 256

Query: 282 -------QFIIGGLPMYAVTFAGYWAYGSSTSTYLLNSVKGPVWVKAMASISAFLQTVIS 341
                  QF +G LP++AVT+ GYWAYG+ST+TYLL+SV GP+WVK MA+++AFLQ+VI+
Sbjct: 257 MMKALYFQFSVGLLPLFAVTWIGYWAYGNSTTTYLLSSVNGPIWVKTMANLAAFLQSVIA 316

Query: 342 LHIFASPMYEYLDTKYGIVGSALNIKNLSFRIGVRGGYMGITTLLSAMLPFIGDFMSLTG 401
           LHIFASPMYEYLDTK+GI GS L I+NLSFR+GVRGGY+   TL+SA+LPF+GDF SLTG
Sbjct: 317 LHIFASPMYEYLDTKFGIKGSPLAIRNLSFRLGVRGGYLAFNTLVSALLPFLGDFESLTG 376

Query: 402 AITTLPLTFILANHMYLVAEKPKLSSLHQLWHWFNVCFFGCMSLAAGVAALRLIAIDSKN 451
           AI+T PLTFILANHMYL A+  KL++L +LWHWFNVCFFGC SLAA VAALRLIA+DSK 
Sbjct: 377 AISTFPLTFILANHMYLRAKNNKLTNLQKLWHWFNVCFFGCASLAAAVAALRLIAVDSKR 436

BLAST of Lsi04G009420 vs. TAIR10
Match: AT3G55740.1 (AT3G55740.1 proline transporter 2)

HSP 1 Score: 599.0 bits (1543), Expect = 2.5e-171
Identity = 304/425 (71.53%), Postives = 347/425 (81.65%), Query Frame = 1

Query: 44  QNMEVAVPETAHQISTDSWFQVGSVLTTGINSAYVLEYSATIMVPLGWIAGVVGFIAATA 103
           +  ++ VPETAHQIS+DSWFQV  VLTTGINSAYVL YS T+MVPLGWI GVVG I ATA
Sbjct: 15  EQFDLEVPETAHQISSDSWFQVAFVLTTGINSAYVLGYSGTVMVPLGWIGGVVGLILATA 74

Query: 104 ISLYANILIAHLHEYGGKRHIRYRDLAAFIYGRRAYSVTWGLQYVNLFMINVGYIIIAGQ 163
           ISLYAN LIA LHE+GGKRHIRYRDLA FIYG++ Y VTWGLQYVNLFMIN G+II+AG 
Sbjct: 75  ISLYANTLIAKLHEFGGKRHIRYRDLAGFIYGKKMYRVTWGLQYVNLFMINCGFIILAGS 134

Query: 164 ALKAVYVLYNDDHAMKLPYFIVIAGFVCALFAISIPHLSALRIWLGFSIVFSFIYIVVAF 223
           ALKAVYVL+ DD  MKLP+FI IAG VCA+FAI IPHLSAL IWLG S + S IYI+VA 
Sbjct: 135 ALKAVYVLFRDDSLMKLPHFIAIAGVVCAIFAIGIPHLSALGIWLGVSTILSIIYIIVAI 194

Query: 224 VLSVQDGVNAPARDYNIPGSSTSKIFTTIGASANLVFVFNTGMLPEI------------- 283
           VLS +DGVN P RDYNI GSS +K+FT  GA+ANLVF FNTGMLPEI             
Sbjct: 195 VLSAKDGVNKPERDYNIQGSSINKLFTITGAAANLVFAFNTGMLPEIQATVKQPVVKNMM 254

Query: 284 -----QFIIGGLPMYAVTFAGYWAYGSSTSTYLLNSVKGPVWVKAMASISAFLQTVISLH 343
                QF +G LPMYAVTF GYWAYGSSTSTYLLNSV GPVWVKA+A+ISAFLQ+VISLH
Sbjct: 255 KALYFQFTVGVLPMYAVTFIGYWAYGSSTSTYLLNSVSGPVWVKALANISAFLQSVISLH 314

Query: 344 IFASPMYEYLDTKYGIVGSALNIKNLSFRIGVRGGYMGITTLLSAMLPFIGDFMSLTGAI 403
           IFASP YEY+DTKYG+ GS L +KNL FR   RG Y+ ++TLLSA+LPF+GDFMSLTGAI
Sbjct: 315 IFASPTYEYMDTKYGVKGSPLAMKNLLFRTVARGSYIAVSTLLSALLPFLGDFMSLTGAI 374

Query: 404 TTLPLTFILANHMYLVAEKPKLSSLHQLWHWFNVCFFGCMSLAAGVAALRLIAIDSKNYN 451
           +T PLTFILANHMYLVA   +LS + +LWHW NVCFFG MSLAA +AA+RLI++DSKN++
Sbjct: 375 STFPLTFILANHMYLVAMNDELSLVQKLWHWLNVCFFGLMSLAAAIAAVRLISVDSKNFH 434

BLAST of Lsi04G009420 vs. TAIR10
Match: AT2G39890.1 (AT2G39890.1 proline transporter 1)

HSP 1 Score: 590.1 bits (1520), Expect = 1.1e-168
Identity = 299/431 (69.37%), Postives = 349/431 (80.97%), Query Frame = 1

Query: 39  NHGDEQNM-EVAVPETAHQISTDSWFQVGSVLTTGINSAYVLEYSATIMVPLGWIAGVVG 98
           N GD  ++ ++ +P+TAHQIS+DSWFQV  VLTTGINSAYVL YS TIMVPLGWI GVVG
Sbjct: 12  NVGDGDDVVDIEIPDTAHQISSDSWFQVAFVLTTGINSAYVLGYSGTIMVPLGWIGGVVG 71

Query: 99  FIAATAISLYANILIAHLHEYGGKRHIRYRDLAAFIYGRRAYSVTWGLQYVNLFMINVGY 158
            + ATAISLYAN LIA LHE+GG+RHIRYRDLA FIYGR+AY +TWGLQYVNLFMIN G+
Sbjct: 72  LLIATAISLYANTLIAKLHEFGGRRHIRYRDLAGFIYGRKAYHLTWGLQYVNLFMINCGF 131

Query: 159 IIIAGQALKAVYVLYNDDHAMKLPYFIVIAGFVCALFAISIPHLSALRIWLGFSIVFSFI 218
           II+AG ALKAVYVL+ DDH MKLP+FI IAG +CA+FAI IPHLSAL +WLG S   S I
Sbjct: 132 IILAGSALKAVYVLFRDDHTMKLPHFIAIAGLICAIFAIGIPHLSALGVWLGVSTFLSLI 191

Query: 219 YIVVAFVLSVQDGVNAPARDYNIPGSSTSKIFTTIGASANLVFVFNTGMLPEI------- 278
           YIVVA VLSV+DGV  P+RDY I GSS SK+FT  GA+ANLVF FNTGMLPEI       
Sbjct: 192 YIVVAIVLSVRDGVKTPSRDYEIQGSSLSKLFTITGAAANLVFAFNTGMLPEIQATVRQP 251

Query: 279 -----------QFIIGGLPMYAVTFAGYWAYGSSTSTYLLNSVKGPVWVKAMASISAFLQ 338
                      QF  G LPMYAVTF GYWAYGSSTSTYLLNSV GP+WVKA+A++SA LQ
Sbjct: 252 VVKNMMKALYFQFTAGVLPMYAVTFIGYWAYGSSTSTYLLNSVNGPLWVKALANVSAILQ 311

Query: 339 TVISLHIFASPMYEYLDTKYGIVGSALNIKNLSFRIGVRGGYMGITTLLSAMLPFIGDFM 398
           +VISLHIFASP YEY+DTKYGI G+   IKNL FRI  RGGY+ ++TL+SA+LPF+GDFM
Sbjct: 312 SVISLHIFASPTYEYMDTKYGIKGNPFAIKNLLFRIMARGGYIAVSTLISALLPFLGDFM 371

Query: 399 SLTGAITTLPLTFILANHMYLVAEKPKLSSLHQLWHWFNVCFFGCMSLAAGVAALRLIAI 451
           SLTGA++T PLTFILANHMY  A+  KL+++ +LWHW NV FF  MS+AA +AA+RLIA+
Sbjct: 372 SLTGAVSTFPLTFILANHMYYKAKNNKLNAMQKLWHWLNVVFFSLMSVAAAIAAVRLIAV 431

BLAST of Lsi04G009420 vs. TAIR10
Match: AT2G36590.1 (AT2G36590.1 proline transporter 3)

HSP 1 Score: 575.5 bits (1482), Expect = 2.9e-164
Identity = 292/425 (68.71%), Postives = 343/425 (80.71%), Query Frame = 1

Query: 44  QNMEVAVPETAHQISTDSWFQVGSVLTTGINSAYVLEYSATIMVPLGWIAGVVGFIAATA 103
           + +++ +P+TAHQIS+DSWFQ   VLTT INSAYVL YS T+MVPLGWI GVVG I ATA
Sbjct: 12  EGVDIEIPDTAHQISSDSWFQAAFVLTTSINSAYVLGYSGTVMVPLGWIGGVVGLILATA 71

Query: 104 ISLYANILIAHLHEYGGKRHIRYRDLAAFIYGRRAYSVTWGLQYVNLFMINVGYIIIAGQ 163
           ISLYAN L+A LHE+GGKRHIRYRDLA FIYGR+AY +TW LQYVNLFMIN G+II+AG 
Sbjct: 72  ISLYANTLVAKLHEFGGKRHIRYRDLAGFIYGRKAYCLTWVLQYVNLFMINCGFIILAGS 131

Query: 164 ALKAVYVLYNDDHAMKLPYFIVIAGFVCALFAISIPHLSALRIWLGFSIVFSFIYIVVAF 223
           ALKAVYVL+ DDHAMKLP+FI IAG +CA+FAI IPHLSAL IWL  S + S IYIVVA 
Sbjct: 132 ALKAVYVLFRDDHAMKLPHFIAIAGLICAVFAIGIPHLSALGIWLAVSTILSLIYIVVAI 191

Query: 224 VLSVQDGVNAPARDYNIPGSSTSKIFTTIGASANLVFVFNTGMLPEI------------- 283
           VLSV+DGV AP+RDY I GS  SK+FT  GA+A LVFVFNTGMLPEI             
Sbjct: 192 VLSVKDGVKAPSRDYEIQGSPLSKLFTITGAAATLVFVFNTGMLPEIQATVKQPVVKNMM 251

Query: 284 -----QFIIGGLPMYAVTFAGYWAYGSSTSTYLLNSVKGPVWVKAMASISAFLQTVISLH 343
                QF +G LPM+AV F GYWAYGSSTS YLLN+V GP+WVKA+A+ISA LQ+VISLH
Sbjct: 252 KALYFQFTVGVLPMFAVVFIGYWAYGSSTSPYLLNNVNGPLWVKALANISAILQSVISLH 311

Query: 344 IFASPMYEYLDTKYGIVGSALNIKNLSFRIGVRGGYMGITTLLSAMLPFIGDFMSLTGAI 403
           IFASP YEY+DTK+GI G+ L +KNL FRI  RGGY+ ++TLLSA+LPF+GDFMSLTGA+
Sbjct: 312 IFASPTYEYMDTKFGIKGNPLALKNLLFRIMARGGYIAVSTLLSALLPFLGDFMSLTGAV 371

Query: 404 TTLPLTFILANHMYLVAEKPKLSSLHQLWHWFNVCFFGCMSLAAGVAALRLIAIDSKNYN 451
           +T PLTFILANHMY  A+  KL++L +L HW NV FF  MS+AA +AALRLIA+DSKN++
Sbjct: 372 STFPLTFILANHMYYKAKNNKLNTLQKLCHWLNVVFFSLMSVAAAIAALRLIALDSKNFH 431

BLAST of Lsi04G009420 vs. TAIR10
Match: AT1G08230.2 (AT1G08230.2 Transmembrane amino acid transporter family protein)

HSP 1 Score: 145.2 bits (365), Expect = 9.7e-35
Identity = 126/434 (29.03%), Postives = 198/434 (45.62%), Query Frame = 1

Query: 58  STDSWFQVGSVLTTGINSAYVLEYSATIMVPLGWIAGVVGFIAATAISLYANILIAHLHE 117
           S  +W+  G  LTT I +  +L         LGW AG+   +   A++ Y+  L++   E
Sbjct: 28  SKGTWWHCGFHLTTSIVAPALLSLPYAFKF-LGWAAGISCLVGGAAVTFYSYTLLSLTLE 87

Query: 118 YG---GKRHIRYRDLAAFIYGRRAYSVTWGLQYVNLFMINVGY-IIIA-----GQALKAV 177
           +    G R++R+RD+A  I      S  WG  YV    + V Y ++IA     GQ LKA+
Sbjct: 88  HHASLGNRYLRFRDMAHHIL-----SPKWGRYYVGPIQMAVCYGVVIANALLGGQCLKAM 147

Query: 178 YVLYNDDHAMKLPYFIVIAGFVCALFAISIPHLSALRIWLGFSIVFSFIYIVVAFVLSVQ 237
           Y++   +  MKL  F++I G +  + A   P   +LR     S++   +Y   A   S+ 
Sbjct: 148 YLVVQPNGEMKLFEFVIIFGCLLLVLA-QFPSFHSLRYINSLSLLLCLLYSASAAAASIY 207

Query: 238 DGV--NAPARDYNIPGSSTSKIFTTIGASANLVFVFNTGMLPEIQFIIGG---------- 297
            G   NAP +DY I G   +++F    A A +   +  G++PEIQ  I            
Sbjct: 208 IGKEPNAPEKDYTIVGDPETRVFGIFNAMAIIATTYGNGIIPEIQATISAPVKGKMMKGL 267

Query: 298 --------LPMYAVTFAGYWAYGSSTS----TYLLNSVKG----PVWVKAMASISAFLQT 357
                   +  + V   GYWA+G   +    T  LN+       P W   + ++   LQ 
Sbjct: 268 CMCYLVVIMTFFTVAITGYWAFGKKANGLIFTNFLNAETNHYFVPTWFIFLVNLFTVLQL 327

Query: 358 VISLHIFASPMYEYLDTKYGI-VGSALNIKNLSFRIGVRGGYMGITTLLSAMLPFIGDFM 417
                ++  P+ + L++          +I+N+  R+ VR  ++ + T+++AMLPF GD  
Sbjct: 328 SAVAVVYLQPINDILESVISDPTKKEFSIRNVIPRLVVRSLFVVMATIVAAMLPFFGDVN 387

Query: 418 SLTGAITTLPLTFILANHMYLVAEKPKLSSLHQLWHWFNVCF---FGCMSLAAGVAALRL 451
           SL GA   +PL F+L    +    KP   S      W N      F C+ + A VAA+R 
Sbjct: 388 SLLGAFGFIPLDFVLPVVFFNFTFKPSKKS---FIFWINTVIAVVFSCLGVIAMVAAVRQ 447

BLAST of Lsi04G009420 vs. TAIR10
Match: AT5G41800.1 (AT5G41800.1 Transmembrane amino acid transporter family protein)

HSP 1 Score: 128.3 bits (321), Expect = 1.2e-29
Identity = 119/433 (27.48%), Postives = 202/433 (46.65%), Query Frame = 1

Query: 58  STDSWFQVGSVLTTGINSAYVLEYSATIMVPLGWIAGVVGFIAATAISLYANILIAHLHE 117
           S   W+  G  LTT I    +L         LGW  G V       ++ YA  L++ + +
Sbjct: 27  SKGEWWHAGFHLTTAIVGPTILTLPYAFR-GLGWWLGFVCLTTMGLVTFYAYYLMSKVLD 86

Query: 118 Y---GGKRHIRYRDLAAFIYGRRAYSVTWGLQ-YVNLFM-------INVGYIIIAGQALK 177
           +    G+RHIR+R+LAA + G        GL  YV +F+       I +G I++AGQ L 
Sbjct: 87  HCEKSGRRHIRFRELAADVLGS-------GLMFYVVIFIQTAINTGIGIGAILLAGQCLD 146

Query: 178 AVYVLYNDDHAMKLPYFIVIAGFVCALFAISIPHLSALRIWLGFSIVFS--FIYIVVAFV 237
            +Y        +KL  FI +   V  + +  +P   +LR     S++ S  + ++VV   
Sbjct: 147 IMYSSLFPQGTLKLYEFIAMVTVVMMVLS-QLPSFHSLRHINCASLLLSLGYTFLVVGAC 206

Query: 238 LSVQDGVNAPARDYNIPGSSTSKIFTTIGASANLVFVFNTGMLPEIQFII---------- 297
           +++    NAP R+Y++  S + K+F+   + + +  +F  G+LPEIQ  +          
Sbjct: 207 INLGLSKNAPKREYSLEHSDSGKVFSAFTSISIIAAIFGNGILPEIQATLAPPATGKMLK 266

Query: 298 GGLPMYAVTF--------AGYWAYGSSTSTYLLNSVK-------GPVWVKAMASISAFLQ 357
           G L  Y+V F        +GYW +G+++S+ +L ++         P+ V  +A I   LQ
Sbjct: 267 GLLLCYSVIFFTFYSAAISGYWVFGNNSSSNILKNLMPDEGPTLAPIVVIGLAVIFVLLQ 326

Query: 358 TVISLHIFASPMYEYLDTKYGIVGSALNIK-NLSFRIGVRGGYMGITTLLSAMLPFIGDF 417
                 +++   YE ++ K       +  K NL  R+ +R  YM     ++AMLPF GD 
Sbjct: 327 LFAIGLVYSQVAYEIMEKKSADTTKGIFSKRNLVPRLILRTLYMAFCGFMAAMLPFFGDI 386

Query: 418 MSLTGAITTLPLTFILANHMYLVAEKPKLSSLHQLWHWFNVCF---FGCMSLAAGVAALR 449
            ++ GA   +PL F+L   +Y +  KP   S     +W N+     F C  L    +++R
Sbjct: 387 NAVVGAFGFIPLDFVLPMLLYNMTYKPTRRS---FTYWINMTIMVVFTCAGLMGAFSSIR 446

BLAST of Lsi04G009420 vs. NCBI nr
Match: gi|659129900|ref|XP_008464902.1| (PREDICTED: proline transporter 1-like [Cucumis melo])

HSP 1 Score: 682.6 bits (1760), Expect = 4.8e-193
Identity = 348/432 (80.56%), Postives = 378/432 (87.50%), Query Frame = 1

Query: 37  PFNHGDEQNMEVAVPETAHQISTDSWFQVGSVLTTGINSAYVLEYSATIMVPLGWIAGVV 96
           P N  DE+ MEVAVPETAHQIS+DSWFQ G VLTTGINSAYVL YS TIMVPLGWI GVV
Sbjct: 3   PINDADEEKMEVAVPETAHQISSDSWFQAGFVLTTGINSAYVLGYSGTIMVPLGWIGGVV 62

Query: 97  GFIAATAISLYANILIAHLHEYGGKRHIRYRDLAAFIYGRRAYSVTWGLQYVNLFMINVG 156
           G IAATAISLYAN LIA LHE+GGKRHIRYRDLA FIYGR+AY++TW LQYVNLFMINVG
Sbjct: 63  GLIAATAISLYANCLIAKLHEFGGKRHIRYRDLAGFIYGRKAYALTWALQYVNLFMINVG 122

Query: 157 YIIIAGQALKAVYVLYNDDHAMKLPYFIVIAGFVCALFAISIPHLSALRIWLGFSIVFSF 216
           +II+AGQALKA YVL++DDH MKLPYFI IAG VCALFAISIPHLSALRIWLGFS VFS 
Sbjct: 123 FIILAGQALKAFYVLFSDDHVMKLPYFIAIAGVVCALFAISIPHLSALRIWLGFSTVFSL 182

Query: 217 IYIVVAFVLSVQDGVNAPARDYNIPGSSTSKIFTTIGASANLVFVFNTGMLPEI------ 276
           +YI+VAF+LS++DGV  PA DY+IPGSSTSKIFTTIGASANLVF FNTGMLPEI      
Sbjct: 183 VYIIVAFILSLRDGVKKPA-DYSIPGSSTSKIFTTIGASANLVFAFNTGMLPEIQATVRQ 242

Query: 277 ------------QFIIGGLPMYAVTFAGYWAYGSSTSTYLLNSVKGPVWVKAMASISAFL 336
                       QF  G LPMYAVTF GYWAYGSSTSTYLLNSV GP+W+KA+A+ISAFL
Sbjct: 243 PVVKNMLKALYFQFTAGVLPMYAVTFIGYWAYGSSTSTYLLNSVNGPIWIKAVANISAFL 302

Query: 337 QTVISLHIFASPMYEYLDTKYGIVGSALNIKNLSFRIGVRGGYMGITTLLSAMLPFIGDF 396
           QTVI+LHIFASPMYEYLDTKYGI GSALNIKNLSFRIGVRGGY+ ITTL+SAMLPF+GDF
Sbjct: 303 QTVIALHIFASPMYEYLDTKYGITGSALNIKNLSFRIGVRGGYLAITTLISAMLPFLGDF 362

Query: 397 MSLTGAITTLPLTFILANHMYLVAEKPKLSSLHQLWHWFNVCFFGCMSLAAGVAALRLIA 451
           MSLTGA++T PLTFILANHMYLVA+K KL+SL +LWHW NVCFFGCMSLAA VAALRLIA
Sbjct: 363 MSLTGAVSTFPLTFILANHMYLVAKKTKLNSLQRLWHWLNVCFFGCMSLAAAVAALRLIA 422

BLAST of Lsi04G009420 vs. NCBI nr
Match: gi|449465033|ref|XP_004150233.1| (PREDICTED: proline transporter 1 [Cucumis sativus])

HSP 1 Score: 674.5 bits (1739), Expect = 1.3e-190
Identity = 345/432 (79.86%), Postives = 375/432 (86.81%), Query Frame = 1

Query: 37  PFNHGDEQNMEVAVPETAHQISTDSWFQVGSVLTTGINSAYVLEYSATIMVPLGWIAGVV 96
           P N  DE+ MEVA P+TAHQIS+DSWFQ G VLTTGINSAYVL YS TIMVPLGWI GVV
Sbjct: 3   PINDVDEEKMEVAAPQTAHQISSDSWFQAGFVLTTGINSAYVLGYSGTIMVPLGWIGGVV 62

Query: 97  GFIAATAISLYANILIAHLHEYGGKRHIRYRDLAAFIYGRRAYSVTWGLQYVNLFMINVG 156
           G IAATAISLYAN LIA LHE+GGKRHIRYRDLA FIYGR AYS+TWGLQY NLFMINVG
Sbjct: 63  GLIAATAISLYANCLIAKLHEFGGKRHIRYRDLAGFIYGRTAYSLTWGLQYANLFMINVG 122

Query: 157 YIIIAGQALKAVYVLYNDDHAMKLPYFIVIAGFVCALFAISIPHLSALRIWLGFSIVFSF 216
           YII+AGQALKA+YVL++DDH MKLPYFI IAG VCALFAISIPHLSAL+IWLGFS VFS 
Sbjct: 123 YIILAGQALKALYVLFSDDHVMKLPYFIAIAGVVCALFAISIPHLSALKIWLGFSTVFSL 182

Query: 217 IYIVVAFVLSVQDGVNAPARDYNIPGSSTSKIFTTIGASANLVFVFNTGMLPEI------ 276
           +YI+VAF+LS++DGV  PA DY++PGSSTSKIFTTIGASANLVF FNTGMLPEI      
Sbjct: 183 VYIIVAFILSLRDGVKTPA-DYSLPGSSTSKIFTTIGASANLVFAFNTGMLPEIQATVRQ 242

Query: 277 ------------QFIIGGLPMYAVTFAGYWAYGSSTSTYLLNSVKGPVWVKAMASISAFL 336
                       QF  G LPMYAVTF GYWAYGSSTSTYLLNSV GP+W+KA A+ISAFL
Sbjct: 243 PVVKNMLKALYFQFTAGVLPMYAVTFIGYWAYGSSTSTYLLNSVNGPIWIKAAANISAFL 302

Query: 337 QTVISLHIFASPMYEYLDTKYGIVGSALNIKNLSFRIGVRGGYMGITTLLSAMLPFIGDF 396
           QTVI+LHIFASPMYEYLDTKYGI GSALNIKNLSFRI VRGGY+ ITTL+SAMLPF+GDF
Sbjct: 303 QTVIALHIFASPMYEYLDTKYGITGSALNIKNLSFRIVVRGGYLAITTLISAMLPFLGDF 362

Query: 397 MSLTGAITTLPLTFILANHMYLVAEKPKLSSLHQLWHWFNVCFFGCMSLAAGVAALRLIA 451
           MSLTGAI+TLPLTFILANHMYLVA+K KL+SL +LWHW NVCFFGCMSLAA VAA+RLIA
Sbjct: 363 MSLTGAISTLPLTFILANHMYLVAKKTKLNSLQRLWHWLNVCFFGCMSLAAAVAAVRLIA 422

BLAST of Lsi04G009420 vs. NCBI nr
Match: gi|743879739|ref|XP_011036024.1| (PREDICTED: proline transporter 1-like isoform X1 [Populus euphratica])

HSP 1 Score: 649.4 bits (1674), Expect = 4.5e-183
Identity = 328/427 (76.81%), Postives = 369/427 (86.42%), Query Frame = 1

Query: 42  DEQNMEVAVPETAHQISTDSWFQVGSVLTTGINSAYVLEYSATIMVPLGWIAGVVGFIAA 101
           DE+N  V +PETAHQIS+DSWFQVG VLTTGINSAYVL YS TIMVPLGWI GV+G I A
Sbjct: 19  DEENSAVEIPETAHQISSDSWFQVGFVLTTGINSAYVLGYSGTIMVPLGWIPGVLGLIIA 78

Query: 102 TAISLYANILIAHLHEYGGKRHIRYRDLAAFIYGRRAYSVTWGLQYVNLFMINVGYIIIA 161
           TAISLYAN LIA LHE+GG+RHIRYRDLA FIYGR+AY +TWGLQYVNLFMIN GYII+A
Sbjct: 79  TAISLYANSLIAELHEFGGRRHIRYRDLAGFIYGRKAYCLTWGLQYVNLFMINTGYIILA 138

Query: 162 GQALKAVYVLYNDDHAMKLPYFIVIAGFVCALFAISIPHLSALRIWLGFSIVFSFIYIVV 221
           G ALKAVY L++DDH MKLPYFI I+GFVCALFA+SIPHLSALR+WLG S VFS IYIVV
Sbjct: 139 GSALKAVYALFSDDHVMKLPYFIAISGFVCALFAMSIPHLSALRLWLGVSTVFSLIYIVV 198

Query: 222 AFVLSVQDGVNAPARDYNIPGSSTSKIFTTIGASANLVFVFNTGMLPEI----------- 281
           AFVLSV+DG+ APARDY+IPG++ SKIFTTIGASANLVF FNTGMLPEI           
Sbjct: 199 AFVLSVKDGIEAPARDYSIPGTTRSKIFTTIGASANLVFAFNTGMLPEIQATIKQPVVSN 258

Query: 282 -------QFIIGGLPMYAVTFAGYWAYGSSTSTYLLNSVKGPVWVKAMASISAFLQTVIS 341
                  QF  G LPMYAVTF GYWAYGSSTSTYLL+SV GPVWVKA+A+ISAFLQTVI+
Sbjct: 259 MMKSLYFQFSAGVLPMYAVTFIGYWAYGSSTSTYLLSSVNGPVWVKALANISAFLQTVIA 318

Query: 342 LHIFASPMYEYLDTKYGIVGSALNIKNLSFRIGVRGGYMGITTLLSAMLPFIGDFMSLTG 401
           LHIFASPMYEYLDTKYGI+GS  +I+NLSFR+GVRGGY+ I TL++A+LPF+GDFMSLTG
Sbjct: 319 LHIFASPMYEYLDTKYGIIGSPFSIRNLSFRVGVRGGYLTINTLVAALLPFLGDFMSLTG 378

Query: 402 AITTLPLTFILANHMYLVAEKPKLSSLHQLWHWFNVCFFGCMSLAAGVAALRLIAIDSKN 451
           AI+T PLTFILANHMYL A+K KL+SL +LWHWFNVCFFG MS+AA V+ALRLIA+DSK 
Sbjct: 379 AISTFPLTFILANHMYLKAKKNKLTSLQKLWHWFNVCFFGLMSIAAAVSALRLIAVDSKT 438

BLAST of Lsi04G009420 vs. NCBI nr
Match: gi|224112617|ref|XP_002316242.1| (proline transporter 2 family protein [Populus trichocarpa])

HSP 1 Score: 647.5 bits (1669), Expect = 1.7e-182
Identity = 329/427 (77.05%), Postives = 369/427 (86.42%), Query Frame = 1

Query: 42  DEQNMEVAVPETAHQISTDSWFQVGSVLTTGINSAYVLEYSATIMVPLGWIAGVVGFIAA 101
           DE N  V +PETAHQIS+DSWFQVG VLTTGINSAYVL YS TIMVPLGWI GVVG I A
Sbjct: 19  DEDNSAVEIPETAHQISSDSWFQVGFVLTTGINSAYVLGYSGTIMVPLGWIPGVVGLIIA 78

Query: 102 TAISLYANILIAHLHEYGGKRHIRYRDLAAFIYGRRAYSVTWGLQYVNLFMINVGYIIIA 161
           TAISLYAN LIA LHE+GG+RHIRYRDLA FIYGR+AYS+TWGLQYVNLFMIN GYII+A
Sbjct: 79  TAISLYANSLIAELHEFGGRRHIRYRDLAGFIYGRKAYSLTWGLQYVNLFMINTGYIILA 138

Query: 162 GQALKAVYVLYNDDHAMKLPYFIVIAGFVCALFAISIPHLSALRIWLGFSIVFSFIYIVV 221
           G ALKAVYVL++DDH MKLPYFI I+GFVCALFA+SIPHLSALR+WLG S VFS IYIVV
Sbjct: 139 GSALKAVYVLFSDDHVMKLPYFIAISGFVCALFAMSIPHLSALRLWLGVSTVFSLIYIVV 198

Query: 222 AFVLSVQDGVNAPARDYNIPGSSTSKIFTTIGASANLVFVFNTGMLPEI----------- 281
           AFVLSV+DG+ APARDY+IPG++ SKIFTTIGASANLVF FNTGMLPEI           
Sbjct: 199 AFVLSVKDGIEAPARDYSIPGTTRSKIFTTIGASANLVFAFNTGMLPEIQATIKQPVVSN 258

Query: 282 -------QFIIGGLPMYAVTFAGYWAYGSSTSTYLLNSVKGPVWVKAMASISAFLQTVIS 341
                  QF  G LPMYAVTF GYWAYGSSTS+YLL+SV GPVWVKA+A+ISAFLQTVI+
Sbjct: 259 MMKSLYFQFSAGVLPMYAVTFIGYWAYGSSTSSYLLSSVNGPVWVKALANISAFLQTVIA 318

Query: 342 LHIFASPMYEYLDTKYGIVGSALNIKNLSFRIGVRGGYMGITTLLSAMLPFIGDFMSLTG 401
           LHIFASPMYEYLDTKYGI+GS  +I+NLSFR+GVRGGY+ I TL++A+LPF+GDFMSLTG
Sbjct: 319 LHIFASPMYEYLDTKYGIIGSPFSIRNLSFRVGVRGGYLTINTLVAALLPFLGDFMSLTG 378

Query: 402 AITTLPLTFILANHMYLVAEKPKLSSLHQLWHWFNVCFFGCMSLAAGVAALRLIAIDSKN 451
           AI+T PLTFILANHMYL A+K KL+SL +LWHWFNV FFG MS+AA V+ALRLIA+DSK 
Sbjct: 379 AISTFPLTFILANHMYLKAKKNKLTSLQKLWHWFNVYFFGLMSIAAAVSALRLIAVDSKT 438

BLAST of Lsi04G009420 vs. NCBI nr
Match: gi|1009121204|ref|XP_015877334.1| (PREDICTED: proline transporter 1-like [Ziziphus jujuba])

HSP 1 Score: 634.8 bits (1636), Expect = 1.1e-178
Identity = 320/427 (74.94%), Postives = 363/427 (85.01%), Query Frame = 1

Query: 42  DEQNMEVAVPETAHQISTDSWFQVGSVLTTGINSAYVLEYSATIMVPLGWIAGVVGFIAA 101
           DE  + V +PETAH+ISTDSWFQVG VLTTGINSAYVL YS TIMVPLGWI GVVG + A
Sbjct: 15  DEDYLSVTIPETAHKISTDSWFQVGFVLTTGINSAYVLGYSGTIMVPLGWICGVVGLLLA 74

Query: 102 TAISLYANILIAHLHEYGGKRHIRYRDLAAFIYGRRAYSVTWGLQYVNLFMINVGYIIIA 161
           TAISLYAN LIA LHE+GGKRHIRYRDLA FIYG+RAYS+TWGLQYVNLFMIN G+II+A
Sbjct: 75  TAISLYANSLIADLHEFGGKRHIRYRDLAGFIYGKRAYSLTWGLQYVNLFMINTGFIILA 134

Query: 162 GQALKAVYVLYNDDHAMKLPYFIVIAGFVCALFAISIPHLSALRIWLGFSIVFSFIYIVV 221
           GQALKAVYVL+ DD+ MKLPYFI IAGFVCALFA+ IPHLSALRIWLGFS  FS +YI+ 
Sbjct: 135 GQALKAVYVLFRDDNVMKLPYFIAIAGFVCALFAVGIPHLSALRIWLGFSTFFSLVYIIT 194

Query: 222 AFVLSVQDGVNAPARDYNIPGSSTSKIFTTIGASANLVFVFNTGMLPEI----------- 281
           A VLS++DG+ A ARDY+IPG+ TSKIFTTIGA+ANLVF FNTGMLPEI           
Sbjct: 195 AVVLSLKDGIKATARDYDIPGTKTSKIFTTIGAAANLVFAFNTGMLPEIQATIRKPVVGN 254

Query: 282 -------QFIIGGLPMYAVTFAGYWAYGSSTSTYLLNSVKGPVWVKAMASISAFLQTVIS 341
                  QF +G LPMYAVTF GYWAYGSSTS+YLLNSV GPVWVKAMA+I+AFLQTVI+
Sbjct: 255 MLKALYFQFTVGVLPMYAVTFMGYWAYGSSTSSYLLNSVNGPVWVKAMANIAAFLQTVIA 314

Query: 342 LHIFASPMYEYLDTKYGIVGSALNIKNLSFRIGVRGGYMGITTLLSAMLPFIGDFMSLTG 401
           LHIFASPMYEY+DTK+GI GSAL+  NLSFRI VRGGY+ I T +SA+LPF+GDFMSLTG
Sbjct: 315 LHIFASPMYEYVDTKFGIKGSALSFHNLSFRIMVRGGYLTINTFVSALLPFLGDFMSLTG 374

Query: 402 AITTLPLTFILANHMYLVAEKPKLSSLHQLWHWFNVCFFGCMSLAAGVAALRLIAIDSKN 451
           AI+T PLTFILANHMY+ A+K KL+S  +LWHW NVCFFGCMS+AA +AALRLIA+DSK 
Sbjct: 375 AISTFPLTFILANHMYMRAKKNKLNSFQKLWHWLNVCFFGCMSVAALIAALRLIAVDSKT 434

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PROT2_ARATH4.4e-17071.53Proline transporter 2 OS=Arabidopsis thaliana GN=PROT2 PE=1 SV=1[more]
PROT1_ARATH2.0e-16769.37Proline transporter 1 OS=Arabidopsis thaliana GN=PROT1 PE=1 SV=1[more]
PROT3_ARATH5.2e-16368.71Proline transporter 3 OS=Arabidopsis thaliana GN=PROT3 PE=1 SV=1[more]
PROT2_ORYSJ1.8e-16068.18Probable proline transporter 2 OS=Oryza sativa subsp. japonica GN=LOC_Os07g01090... [more]
PROT1_ORYSJ2.2e-15860.93Proline transporter 1 OS=Oryza sativa subsp. japonica GN=PROT1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KQE7_CUCSA9.1e-19179.86Uncharacterized protein OS=Cucumis sativus GN=Csa_5G197090 PE=4 SV=1[more]
B9HT23_POPTR1.2e-18277.05Proline transporter 2 family protein OS=Populus trichocarpa GN=POPTR_0010s20180g... [more]
B9HHJ8_POPTR1.8e-17875.18Proline transporter 2 family protein OS=Populus trichocarpa GN=POPTR_0008s06340g... [more]
W9R1P4_9ROSA3.0e-17874.24Lysine histidine transporter 1 OS=Morus notabilis GN=L484_001878 PE=3 SV=1[more]
B9SWN5_RICCO7.5e-17773.54Amino acid transporter, putative OS=Ricinus communis GN=RCOM_0498260 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G55740.12.5e-17171.53 proline transporter 2[more]
AT2G39890.11.1e-16869.37 proline transporter 1[more]
AT2G36590.12.9e-16468.71 proline transporter 3[more]
AT1G08230.29.7e-3529.03 Transmembrane amino acid transporter family protein[more]
AT5G41800.11.2e-2927.48 Transmembrane amino acid transporter family protein[more]
Match NameE-valueIdentityDescription
gi|659129900|ref|XP_008464902.1|4.8e-19380.56PREDICTED: proline transporter 1-like [Cucumis melo][more]
gi|449465033|ref|XP_004150233.1|1.3e-19079.86PREDICTED: proline transporter 1 [Cucumis sativus][more]
gi|743879739|ref|XP_011036024.1|4.5e-18376.81PREDICTED: proline transporter 1-like isoform X1 [Populus euphratica][more]
gi|224112617|ref|XP_002316242.1|1.7e-18277.05proline transporter 2 family protein [Populus trichocarpa][more]
gi|1009121204|ref|XP_015877334.1|1.1e-17874.94PREDICTED: proline transporter 1-like [Ziziphus jujuba][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR013057AA_transpt_TM
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0003333 amino acid transmembrane transport
biological_process GO:0008150 biological_process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0005575 cellular_component
molecular_function GO:0015171 amino acid transmembrane transporter activity
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G009420.1Lsi04G009420.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013057Amino acid transporter, transmembrane domainPFAMPF01490Aa_transcoord: 59..271
score: 3.1E-37coord: 273..434
score: 3.5
NoneNo IPR availablePANTHERPTHR22950AMINO ACID TRANSPORTERcoord: 1..450
score: 7.5E
NoneNo IPR availablePANTHERPTHR22950:SF48PROLINE TRANSPORTER 1-RELATEDcoord: 1..450
score: 7.5E

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi04G009420Bottle gourd (USVL1VR-Ls)lsilsiB127
Lsi04G009420Bottle gourd (USVL1VR-Ls)lsilsiB129
Lsi04G009420Cucumber (Gy14) v1cgylsiB217
Lsi04G009420Cucumber (Gy14) v1cgylsiB299
Lsi04G009420Cucurbita maxima (Rimu)cmalsiB616
Lsi04G009420Cucurbita maxima (Rimu)cmalsiB676
Lsi04G009420Cucurbita maxima (Rimu)cmalsiB686
Lsi04G009420Cucurbita maxima (Rimu)cmalsiB756
Lsi04G009420Cucurbita moschata (Rifu)cmolsiB267
Lsi04G009420Cucurbita moschata (Rifu)cmolsiB603
Lsi04G009420Cucurbita moschata (Rifu)cmolsiB669
Lsi04G009420Cucurbita moschata (Rifu)cmolsiB670
Lsi04G009420Cucurbita moschata (Rifu)cmolsiB679
Lsi04G009420Cucurbita moschata (Rifu)cmolsiB751
Lsi04G009420Cucurbita pepo (Zucchini)cpelsiB152
Lsi04G009420Cucurbita pepo (Zucchini)cpelsiB358
Lsi04G009420Wild cucumber (PI 183967)cpilsiB052
Lsi04G009420Wild cucumber (PI 183967)cpilsiB195
Lsi04G009420Wild cucumber (PI 183967)cpilsiB459
Lsi04G009420Cucumber (Chinese Long) v2culsiB052
Lsi04G009420Cucumber (Chinese Long) v2culsiB196
Lsi04G009420Cucumber (Chinese Long) v2culsiB452
Lsi04G009420Melon (DHL92) v3.5.1lsimeB283
Lsi04G009420Melon (DHL92) v3.5.1lsimeB300
Lsi04G009420Melon (DHL92) v3.5.1lsimeB308
Lsi04G009420Melon (DHL92) v3.5.1lsimeB332
Lsi04G009420Watermelon (Charleston Gray)lsiwcgB256
Lsi04G009420Watermelon (97103) v1lsiwmB287
Lsi04G009420Watermelon (97103) v1lsiwmB338
Lsi04G009420Cucumber (Gy14) v2cgyblsiB418
Lsi04G009420Melon (DHL92) v3.6.1lsimedB297
Lsi04G009420Melon (DHL92) v3.6.1lsimedB313
Lsi04G009420Silver-seed gourdcarlsiB239
Lsi04G009420Silver-seed gourdcarlsiB405
Lsi04G009420Silver-seed gourdcarlsiB608
Lsi04G009420Silver-seed gourdcarlsiB916
Lsi04G009420Cucumber (Chinese Long) v3cuclsiB481
Lsi04G009420Watermelon (97103) v2lsiwmbB274
Lsi04G009420Wax gourdlsiwgoB366
Lsi04G009420Wax gourdlsiwgoB385