Lsi04G007990 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi04G007990
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Ferric reduction oxidase 2
Location	chr04 : 7569945 .. 7571335 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ACTTCTCTTTTGATGTATACATTCCCCATTTTGTTCATTGCTATTTTGGGTTGTGTTTACATTCATTTGGAACAAAAATGTGAAGATCGAAACGTGGAAACCAGGTAAATGATAACGGATATAATATAGAGATTGTTTGATACCATTTTTGTTTATCTAAGTTTTCATAAAAATATTGGCTTCTTACCTTGTTAAGTAACATTCTTATCTTTTGTTTCCAATTTTTAGGAAATACAAATGTTATTTTAACTATTTTTGAGAAAATTGATGACTTCTTACTTGTTAAGTAACGTTCTTATTTTTGTTTTCAATGTTTTAGAAATATAAATGTTTTTGTAACTATTTTTGCTCATTTTTCAAAGAAAGGCCTAAAAGTTCTATACATTTTTTAAACAACATGATCTTATTTGTTTGTTTCTCCCAGATGACCCATCTGATAACGATTTCATATTTACTTCTCTCCTTAATTTTCTTACTAAATAGTTTTCACATAGTTTCTGAAAGAAAATTTGAATTCTTTGTAAAATTCTAACATTAATAACAACTTTTTAAACTGTTTTTTTTTACTTATAAAATAATTATAAACCAAATAGACATAACACAATATTTTTATTATTTTATTTAAAAAAAATTTTGGAAGAGTGCAAGAAATAAAGAAATCACAAAGTGTATTTCTCTTCTTCTTAAAAAAACAAAAACAAAAACAAATTAATTATAAATAAATAAATAAAACAATTATAAACATTACCAAACAAGCCCTATATATAAAAAACTTATCAGTTTTCAAAGATTTTAAGAATAAGTTTTTCTCCAATTCTCGACAGAAAAAGAAAAAATCATCCTGGTTTAACGACGTGGAAGCAACCAGCAATCATGAAAGGGCCACTCGGGATCGTCTCATGGACAGAACTAGCCCTGCTAGCCATGTTCATGACACTCTTAGTGTGGTCTTTTGCCACATATTTACACAACAGCTTCGTCAAAATCACTCCACAATCAGCAGCAATGGACGGAGAGAAAGTGTAAGAAAAACTTCTACTAATTATATATTATGATTCATAGTTATTCTTTGTTGAAGTTCTAAGTAAGTAATCAATCAGAATTTTGATTTTGATTTTGAGGGCAGGTGGGAAAGTAGATTGGGAACTGCAGGGTTTTGGTTGGGAATTGTTGGGAATATTTGTTTGGTCTTTTTGTTCTTTCCGGTGGCTCGTGGGTCGTCACTACTACCGCTGCTCGGTCTAACCTCGGAGGGTTGCATTAAATATCACATATGGCTCGGCCACATGACGATGGCTTTCTTCTCCGCTCATGGCATTTGCTTCGTTATCTATTGGGCTGCCACTAATAATCTTTCTCAGGTGAGTTCTCATTCTAACAATAATACTTAG

mRNA sequence

ACTTCTCTTTTGATGTATACATTCCCCATTTTGTTCATTGCTATTTTGGGTTGTGTTTACATTCATTTGGAACAAAAATGTGAAGATCGAAACGTGGAAACCAGAAAAAGAAAAAATCATCCTGGTTTAACGACGTGGAAGCAACCAGCAATCATGAAAGGGCCACTCGGGATCGTCTCATGGACAGAACTAGCCCTGCTAGCCATGTTCATGACACTCTTAGTGTGGTCTTTTGCCACATATTTACACAACAGCTTCGTCAAAATCACTCCACAATCAGCAGCAATGGACGGAGAGAAAGTGTGGGAAAGTAGATTGGGAACTGCAGGGTTTTGGTTGGGAATTGTTGGGAATATTTGTTTGGTCTTTTTGTTCTTTCCGGTGGCTCGTGGGTCGTCACTACTACCGCTGCTCGGTCTAACCTCGGAGGGTTGCATTAAATATCACATATGGCTCGGCCACATGACGATGGCTTTCTTCTCCGCTCATGGCATTTGCTTCGTTATCTATTGGGCTGCCACTAATAATCTTTCTCAGGTGAGTTCTCATTCTAACAATAATACTTAG

Coding sequence (CDS)

ACTTCTCTTTTGATGTATACATTCCCCATTTTGTTCATTGCTATTTTGGGTTGTGTTTACATTCATTTGGAACAAAAATGTGAAGATCGAAACGTGGAAACCAGAAAAAGAAAAAATCATCCTGGTTTAACGACGTGGAAGCAACCAGCAATCATGAAAGGGCCACTCGGGATCGTCTCATGGACAGAACTAGCCCTGCTAGCCATGTTCATGACACTCTTAGTGTGGTCTTTTGCCACATATTTACACAACAGCTTCGTCAAAATCACTCCACAATCAGCAGCAATGGACGGAGAGAAAGTGTGGGAAAGTAGATTGGGAACTGCAGGGTTTTGGTTGGGAATTGTTGGGAATATTTGTTTGGTCTTTTTGTTCTTTCCGGTGGCTCGTGGGTCGTCACTACTACCGCTGCTCGGTCTAACCTCGGAGGGTTGCATTAAATATCACATATGGCTCGGCCACATGACGATGGCTTTCTTCTCCGCTCATGGCATTTGCTTCGTTATCTATTGGGCTGCCACTAATAATCTTTCTCAGGTGAGTTCTCATTCTAACAATAATACTTAG

Protein sequence

TSLLMYTFPILFIAILGCVYIHLEQKCEDRNVETRKRKNHPGLTTWKQPAIMKGPLGIVSWTELALLAMFMTLLVWSFATYLHNSFVKITPQSAAMDGEKVWESRLGTAGFWLGIVGNICLVFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGHMTMAFFSAHGICFVIYWAATNNLSQVSSHSNNNT

Homology

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BLAST of Lsi04G007990 vs. Swiss-Prot
Match: FRO2_ARATH (Ferric reduction oxidase 2 OS=Arabidopsis thaliana GN=FRO2 PE=1 SV=2)

HSP 1 Score: 207.6 bits (527), Expect = 1.2e-52
Identity = 97/181 (53.59%), Postives = 134/181 (74.03%), Query Frame = 1


Query: 1   TSLLMYTFPILFIAILGCVYIHLEQKCEDRNVETRKRKNHPGL-TTWKQPAIMKGPLGIV 60
           T+L MY FP++ +A LGCVY+H + +    +++   R+   G+ +  ++P ++KGPLGIV
Sbjct: 73  TTLFMYMFPMMVVACLGCVYLHFKNRKSPHHID---RETKGGVWSKLRKPMLVKGPLGIV 132

Query: 61  SWTELALLAMFMTLLVWSFATYLHNSFVKITPQSAAMDGEKVWESRLGTAGFWLGIVGNI 120
           S TE+  LAMF+ LL+W F TYL NSF  ITP+SAA   E +W+++L +A   LG++GNI
Sbjct: 133 SVTEITFLAMFVALLLWCFITYLRNSFATITPKSAAAHDESLWQAKLESAALRLGLIGNI 192

Query: 121 CLVFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGHMTMAFFSAHGICFVIYWAATNNLSQ 180
           CL FLF PVARGSSLLP +GLTSE  IKYHIWLGHM MA F+ HG+C++IYWA+ + +SQ
Sbjct: 193 CLAFLFLPVARGSSLLPAMGLTSESSIKYHIWLGHMVMALFTVHGLCYIIYWASMHEISQ 250

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BLAST of Lsi04G007990 vs. Swiss-Prot
Match: FRO1_ARATH (Probable ferric reduction oxidase 1 OS=Arabidopsis thaliana GN=FRO1 PE=2 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 5.1e-48
Identity = 87/176 (49.43%), Postives = 121/176 (68.75%), Query Frame = 1


Query: 2   SLLMYTFPILFIAILGCVYIHLEQKCEDRNVETRKRKNHPGLTTWKQPAIMKGPLGIVSW 61
           +LL+Y FP++ +A LGC+Y+HL++        +   K        ++P ++ GPLGIV+ 
Sbjct: 62  NLLVYMFPMILLAFLGCIYLHLKKSTTVNQFNSGVEKKRAKFGALRRPMLVNGPLGIVTV 121

Query: 62  TELALLAMFMTLLVWSFATYLHNSFVKITPQSAAMDGEKVWESRLGTAGFWLGIVGNICL 121
           TE+  L MFM LL+WS A Y++ +FV +T +SAA DG  +W++RL      +GIVGNICL
Sbjct: 122 TEVMFLTMFMALLLWSLANYMYRTFVNVTSESAATDGNNLWQARLDLIAVRIGIVGNICL 181

Query: 122 VFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGHMTMAFFSAHGICFVIYWAATNNL 178
            FLF+PVARGSSLL  +GLTSE  IKYHIWLGH+ M  F++HG+C+ IYW + N L
Sbjct: 182 AFLFYPVARGSSLLAAVGLTSESSIKYHIWLGHLVMIIFTSHGLCYFIYWISKNQL 237

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BLAST of Lsi04G007990 vs. Swiss-Prot
Match: FRO3_ARATH (Ferric reduction oxidase 3, mitochondrial OS=Arabidopsis thaliana GN=FRO3 PE=2 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 1.4e-45
Identity = 88/180 (48.89%), Postives = 125/180 (69.44%), Query Frame = 1


Query: 2   SLLMYTFPILFIAILGCVYIHLEQKCEDRNVETRK-RKNHPGLTTWKQPAIMKGPLGIVS 61
           +LL+Y FP++ +A LG +Y+HL+++       +R  RK        K+P ++K  LGIV+
Sbjct: 85  NLLVYMFPMILLASLGSIYLHLKKQTRVNQFNSRMDRKKIDKFGALKRPMLVKAGLGIVT 144

Query: 62  WTELALLAMFMTLLVWSFATYLHNSFVKITPQSAAMDGEKVWESRLGTAGFWLGIVGNIC 121
            TE+  L MFM LL+WS A Y +++FV ITPQS   DG+ +W++RL +    LG+ GNIC
Sbjct: 145 VTEVMFLMMFMALLLWSLANYFYHTFVTITPQSLPTDGDNLWQARLDSIAVRLGLTGNIC 204

Query: 122 LVFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGHMTMAFFSAHGICFVIYWAATNNLSQV 181
           L FLF+PVARGSSLL  +GLTSE   KYHIWLG++ M  F++HG+C+ IYW +TN +SQ+
Sbjct: 205 LGFLFYPVARGSSLLAAVGLTSESSTKYHIWLGNLVMTLFTSHGLCYCIYWISTNQVSQM 264

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BLAST of Lsi04G007990 vs. Swiss-Prot
Match: FRO4_ARATH (Ferric reduction oxidase 4 OS=Arabidopsis thaliana GN=FRO4 PE=2 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 3.6e-33
Identity = 74/179 (41.34%), Postives = 108/179 (60.34%), Query Frame = 1


Query: 1   TSLLMYTFPILFIAILGCVYIHLEQKCEDRNVETRKRKNHPGLTTWKQPAIMKGPLGIVS 60
           T+L++ T P++FIA+L CVY+H+++K      E + ++         +  ++  PLGIV+
Sbjct: 53  TNLVLLTVPMMFIAVLSCVYLHIQKKPTQPQREWKLKR------IMGRVIMVMNPLGIVT 112

Query: 61  WTELALLAMFMTLLVWSFATYLHNSFVKITPQSAAMDGEKVWESRLGTAGFWLGIVGNIC 120
            TEL    +F+ LL WS   YL+ S+          D  K+W+++    G  +G VGNIC
Sbjct: 113 ATELTFSLLFVALLAWSLYNYLYLSYHVHLHND---DNAKIWQAKFRAFGLRIGYVGNIC 172

Query: 121 LVFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGHMTMAFFSAHGICFVIYWAATNNLSQ 180
             FLFFPV R S++LPL+GLTSE  IKYHIWLGH++   F  H + F+IYWA  N L +
Sbjct: 173 WAFLFFPVTRASTILPLVGLTSESSIKYHIWLGHVSNFCFLVHTVVFLIYWAMINKLME 222

[Close]

BLAST of Lsi04G007990 vs. Swiss-Prot
Match: FRO5_ARATH (Ferric reduction oxidase 5 OS=Arabidopsis thaliana GN=FRO5 PE=2 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 3.7e-30
Identity = 74/188 (39.36%), Postives = 110/188 (58.51%), Query Frame = 1


Query: 2   SLLMYTFPILFIAILGCVYIHLEQ---------KCEDRNVETRKRKNHPGLTTWKQPAIM 61
           +L++ T P++FIA+L CVY+H ++         KC +  V+ R  +            ++
Sbjct: 54  NLVLLTVPMMFIAVLSCVYLHTQKQPSQTQSLYKCREWKVKGRMGR----------VMMV 113

Query: 62  KGPLGIVSWTELALLAMFMTLLVWSFATYLHNSF-VKITPQSAAMDGEKVWESRLGTAGF 121
             PLGIV+ TEL    +F+ LLVW+ + YL+ S+ V +     A D  + W+++    G 
Sbjct: 114 MNPLGIVTATELTFSLLFLALLVWALSNYLYLSYHVHLHNHDNANDMCR-WQAKFRAFGL 173

Query: 122 WLGIVGNICLVFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGHMTMAFFSAHGICFVIYW 180
            +G VG+ C  FLFFPV R S++LPL+GLTSE  IKYHIWLGH++   F  H + F+IYW
Sbjct: 174 RIGYVGHYCWAFLFFPVTRASTILPLVGLTSESSIKYHIWLGHVSNFCFLVHTVVFLIYW 230

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BLAST of Lsi04G007990 vs. TrEMBL
Match: Q6PND7_CUCSA (Ferric reductase OS=Cucumis sativus PE=2 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 6.3e-77
Identity = 145/180 (80.56%), Postives = 155/180 (86.11%), Query Frame = 1


Query: 1   TSLLMYTFPILFIAILGCVYIHLEQKCEDRNVETRKRKNHPGLTTWKQPAIMKGPLGIVS 60
           TSLLMYTFPILFIAILGCVY HLE+K  D NV+TRK   H GL  WKQPAI+KGPLGIVS
Sbjct: 57  TSLLMYTFPILFIAILGCVYNHLEKKRSDPNVKTRKGTKHHGLAMWKQPAIVKGPLGIVS 116

Query: 61  WTELALLAMFMTLLVWSFATYLHNSFVKITPQSAAMDGEKVWESRLGTAGFWLGIVGNIC 120
           WTEL LL MF+ LLVWSFATYLHNSF+           EK+WE RLG+AGFWLGIVGNIC
Sbjct: 117 WTELTLLTMFIILLVWSFATYLHNSFIT----------EKMWEIRLGSAGFWLGIVGNIC 176

Query: 121 LVFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGHMTMAFFSAHGICFVIYWAATNNLSQV 180
           LVFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGHMTMAFFSAHGICF+IYWAATNN+SQ+
Sbjct: 177 LVFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGHMTMAFFSAHGICFLIYWAATNNISQM 226

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BLAST of Lsi04G007990 vs. TrEMBL
Match: A0A0A0KLI0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G175770 PE=4 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 3.7e-61
Identity = 117/146 (80.14%), Postives = 125/146 (85.62%), Query Frame = 1


Query: 35  RKRKNHPGLTTWKQPAIMKGPLGIVSWTELALLAMFMTLLVWSFATYLHNSFVKITPQSA 94
           RK   H GL  WKQPAI+KGPLGIVSWTEL LL MF+ LLVWSFATYLHNSF+       
Sbjct: 14  RKGTKHHGLAMWKQPAIVKGPLGIVSWTELTLLTMFIILLVWSFATYLHNSFIT------ 73

Query: 95  AMDGEKVWESRLGTAGFWLGIVGNICLVFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGH 154
               EK+WE RLG+AGFWLGIVGNICLVFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGH
Sbjct: 74  ----EKMWEIRLGSAGFWLGIVGNICLVFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGH 133

Query: 155 MTMAFFSAHGICFVIYWAATNNLSQV 181
           MTMAFFSAHGICF+IYWAATNN+SQ+
Sbjct: 134 MTMAFFSAHGICFLIYWAATNNISQM 149

[Close]

BLAST of Lsi04G007990 vs. TrEMBL
Match: F5BSM2_LOTJA (Ferric reductase OS=Lotus japonicus GN=FRO1 PE=2 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 2.4e-60
Identity = 113/178 (63.48%), Postives = 137/178 (76.97%), Query Frame = 1


Query: 3   LLMYTFPILFIAILGCVYIHLEQKCEDRNVETRKRKNHPGLTTWKQPAIMKGPLGIVSWT 62
           +L +TFP+LFIA+LGCVY+H            +K  N P  T WK+P ++KGPLGIVS T
Sbjct: 69  ILTFTFPVLFIAVLGCVYLH----------TAKKSSNGPEATIWKRPVLVKGPLGIVSST 128

Query: 63  ELALLAMFMTLLVWSFATYLHNSFVKITPQSAAMDGEKVWESRLGTAGFWLGIVGNICLV 122
           ELA L MF+ LLVWSFATYLHN F KIT +SAA DGEKVWE +L  A   LG++GNICL 
Sbjct: 129 ELAFLLMFIALLVWSFATYLHNGFAKITQKSAAADGEKVWEEKLEXAALRLGLIGNICLA 188

Query: 123 FLFFPVARGSSLLPLLGLTSEGCIKYHIWLGHMTMAFFSAHGICFVIYWAATNNLSQV 181
           FL+FPVARGS++LPLLGLTSE CIKYHIWLGH+ M  F++HGIC++IYWAAT  +SQ+
Sbjct: 189 FLYFPVARGSTVLPLLGLTSESCIKYHIWLGHVVMTLFTSHGICYIIYWAATTQISQM 236

[Close]

BLAST of Lsi04G007990 vs. TrEMBL
Match: F5BSM3_LOTJA (Ferric reductase OS=Lotus japonicus GN=FRO1 PE=2 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 3.1e-60
Identity = 113/176 (64.20%), Postives = 136/176 (77.27%), Query Frame = 1


Query: 3   LLMYTFPILFIAILGCVYIHLEQKCEDRNVETRKRKNHPGLTTWKQPAIMKGPLGIVSWT 62
           +L +TFP+LFIA+LGCVY+H            +K  N P  T WK+P ++KGPLGIVS T
Sbjct: 69  ILTFTFPVLFIAVLGCVYLH----------TAKKSSNGPEATIWKRPVLVKGPLGIVSST 128

Query: 63  ELALLAMFMTLLVWSFATYLHNSFVKITPQSAAMDGEKVWESRLGTAGFWLGIVGNICLV 122
           ELA L MF+ LLVWSFATYLHN F KIT +SAA DGEKVWE +L TA   LG++GNICL 
Sbjct: 129 ELAFLLMFIALLVWSFATYLHNGFAKITQKSAAADGEKVWEEKLETAALRLGLIGNICLA 188

Query: 123 FLFFPVARGSSLLPLLGLTSEGCIKYHIWLGHMTMAFFSAHGICFVIYWAATNNLS 179
           FL+FPVARGS++LPLLGLTSE CIKYHIWLGH+ M  F++HGIC++IYWAAT  +S
Sbjct: 189 FLYFPVARGSTVLPLLGLTSESCIKYHIWLGHVVMTLFTSHGICYIIYWAATTQIS 234

[Close]

BLAST of Lsi04G007990 vs. TrEMBL
Match: W9SCD8_9ROSA (Ferric reduction oxidase 2 OS=Morus notabilis GN=L484_021470 PE=4 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 9.1e-60
Identity = 109/179 (60.89%), Postives = 139/179 (77.65%), Query Frame = 1


Query: 2   SLLMYTFPILFIAILGCVYIHLEQKCEDRNVETRKRKNHPGLTTWKQPAIMKGPLGIVSW 61
           +LL+YTFP+L  A+LGCVYIHL +K +  +   +  KNH     WK+P ++KGP GIVS 
Sbjct: 66  TLLLYTFPVLLFAVLGCVYIHLGRKLDGHDHNVQSGKNHR-FAVWKRPILVKGPQGIVSS 125

Query: 62  TELALLAMFMTLLVWSFATYLHNSFVKITPQSAAMDGEKVWESRLGTAGFWLGIVGNICL 121
            EL    MF+ LLVWSF+TYLHNSF KITP+SAA DGEKVWES+L +A   LG++GNICL
Sbjct: 126 IELTFFVMFIALLVWSFSTYLHNSFAKITPKSAAKDGEKVWESKLDSAALRLGLIGNICL 185

Query: 122 VFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGHMTMAFFSAHGICFVIYWAATNNLSQV 181
            FLFFPVARGSS+LPL GLTSE  IKYHIWLGH+ +  F+AHG+C++I+W  T+++SQ+
Sbjct: 186 AFLFFPVARGSSVLPLFGLTSEASIKYHIWLGHLVLTLFTAHGLCYIIFWGVTHDISQM 243

[Close]

BLAST of Lsi04G007990 vs. TAIR10
Match: AT1G01580.1 (AT1G01580.1 ferric reduction oxidase 2)

HSP 1 Score: 207.6 bits (527), Expect = 6.7e-54
Identity = 97/181 (53.59%), Postives = 134/181 (74.03%), Query Frame = 1


Query: 1   TSLLMYTFPILFIAILGCVYIHLEQKCEDRNVETRKRKNHPGL-TTWKQPAIMKGPLGIV 60
           T+L MY FP++ +A LGCVY+H + +    +++   R+   G+ +  ++P ++KGPLGIV
Sbjct: 73  TTLFMYMFPMMVVACLGCVYLHFKNRKSPHHID---RETKGGVWSKLRKPMLVKGPLGIV 132

Query: 61  SWTELALLAMFMTLLVWSFATYLHNSFVKITPQSAAMDGEKVWESRLGTAGFWLGIVGNI 120
           S TE+  LAMF+ LL+W F TYL NSF  ITP+SAA   E +W+++L +A   LG++GNI
Sbjct: 133 SVTEITFLAMFVALLLWCFITYLRNSFATITPKSAAAHDESLWQAKLESAALRLGLIGNI 192

Query: 121 CLVFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGHMTMAFFSAHGICFVIYWAATNNLSQ 180
           CL FLF PVARGSSLLP +GLTSE  IKYHIWLGHM MA F+ HG+C++IYWA+ + +SQ
Sbjct: 193 CLAFLFLPVARGSSLLPAMGLTSESSIKYHIWLGHMVMALFTVHGLCYIIYWASMHEISQ 250

[Close]

BLAST of Lsi04G007990 vs. TAIR10
Match: AT1G01590.1 (AT1G01590.1 ferric reduction oxidase 1)

HSP 1 Score: 192.2 bits (487), Expect = 2.9e-49
Identity = 87/176 (49.43%), Postives = 121/176 (68.75%), Query Frame = 1


Query: 2   SLLMYTFPILFIAILGCVYIHLEQKCEDRNVETRKRKNHPGLTTWKQPAIMKGPLGIVSW 61
           +LL+Y FP++ +A LGC+Y+HL++        +   K        ++P ++ GPLGIV+ 
Sbjct: 62  NLLVYMFPMILLAFLGCIYLHLKKSTTVNQFNSGVEKKRAKFGALRRPMLVNGPLGIVTV 121

Query: 62  TELALLAMFMTLLVWSFATYLHNSFVKITPQSAAMDGEKVWESRLGTAGFWLGIVGNICL 121
           TE+  L MFM LL+WS A Y++ +FV +T +SAA DG  +W++RL      +GIVGNICL
Sbjct: 122 TEVMFLTMFMALLLWSLANYMYRTFVNVTSESAATDGNNLWQARLDLIAVRIGIVGNICL 181

Query: 122 VFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGHMTMAFFSAHGICFVIYWAATNNL 178
            FLF+PVARGSSLL  +GLTSE  IKYHIWLGH+ M  F++HG+C+ IYW + N L
Sbjct: 182 AFLFYPVARGSSLLAAVGLTSESSIKYHIWLGHLVMIIFTSHGLCYFIYWISKNQL 237

[Close]

BLAST of Lsi04G007990 vs. TAIR10
Match: AT1G23020.2 (AT1G23020.2 ferric reduction oxidase 3)

HSP 1 Score: 184.1 bits (466), Expect = 7.9e-47
Identity = 88/180 (48.89%), Postives = 125/180 (69.44%), Query Frame = 1


Query: 2   SLLMYTFPILFIAILGCVYIHLEQKCEDRNVETRK-RKNHPGLTTWKQPAIMKGPLGIVS 61
           +LL+Y FP++ +A LG +Y+HL+++       +R  RK        K+P ++K  LGIV+
Sbjct: 85  NLLVYMFPMILLASLGSIYLHLKKQTRVNQFNSRMDRKKIDKFGALKRPMLVKAGLGIVT 144

Query: 62  WTELALLAMFMTLLVWSFATYLHNSFVKITPQSAAMDGEKVWESRLGTAGFWLGIVGNIC 121
            TE+  L MFM LL+WS A Y +++FV ITPQS   DG+ +W++RL +    LG+ GNIC
Sbjct: 145 VTEVMFLMMFMALLLWSLANYFYHTFVTITPQSLPTDGDNLWQARLDSIAVRLGLTGNIC 204

Query: 122 LVFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGHMTMAFFSAHGICFVIYWAATNNLSQV 181
           L FLF+PVARGSSLL  +GLTSE   KYHIWLG++ M  F++HG+C+ IYW +TN +SQ+
Sbjct: 205 LGFLFYPVARGSSLLAAVGLTSESSTKYHIWLGNLVMTLFTSHGLCYCIYWISTNQVSQM 264

[Close]

BLAST of Lsi04G007990 vs. TAIR10
Match: AT5G23980.1 (AT5G23980.1 ferric reduction oxidase 4)

HSP 1 Score: 142.9 bits (359), Expect = 2.0e-34
Identity = 74/179 (41.34%), Postives = 108/179 (60.34%), Query Frame = 1


Query: 1   TSLLMYTFPILFIAILGCVYIHLEQKCEDRNVETRKRKNHPGLTTWKQPAIMKGPLGIVS 60
           T+L++ T P++FIA+L CVY+H+++K      E + ++         +  ++  PLGIV+
Sbjct: 53  TNLVLLTVPMMFIAVLSCVYLHIQKKPTQPQREWKLKR------IMGRVIMVMNPLGIVT 112

Query: 61  WTELALLAMFMTLLVWSFATYLHNSFVKITPQSAAMDGEKVWESRLGTAGFWLGIVGNIC 120
            TEL    +F+ LL WS   YL+ S+          D  K+W+++    G  +G VGNIC
Sbjct: 113 ATELTFSLLFVALLAWSLYNYLYLSYHVHLHND---DNAKIWQAKFRAFGLRIGYVGNIC 172

Query: 121 LVFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGHMTMAFFSAHGICFVIYWAATNNLSQ 180
             FLFFPV R S++LPL+GLTSE  IKYHIWLGH++   F  H + F+IYWA  N L +
Sbjct: 173 WAFLFFPVTRASTILPLVGLTSESSIKYHIWLGHVSNFCFLVHTVVFLIYWAMINKLME 222

[Close]

BLAST of Lsi04G007990 vs. TAIR10
Match: AT5G49730.1 (AT5G49730.1 ferric reduction oxidase 6)

HSP 1 Score: 114.8 bits (286), Expect = 5.9e-26
Identity = 60/180 (33.33%), Postives = 95/180 (52.78%), Query Frame = 1


Query: 1   TSLLMYTFPILFIAILGCVYIHLEQKCEDRNVETRKRKNHPGLTTWKQPAIMKGPLGIVS 60
           T  L+++ PIL I++L  +Y+ +    ED+    +K    P    W  P ++ GP G+VS
Sbjct: 70  TMFLIFSCPILVISVLASLYLIISG--EDKVFTKKKISKFPRFRLWTFPVLVDGPFGVVS 129

Query: 61  WTELALLAMFMTLLVWSFATYLHNSFVKITPQSAAMDGEKVWESRLGTAGFWLGIVGNIC 120
             E   + +F    +WS   Y   +   +           +    L   G  LG++G +C
Sbjct: 130 AAEFLGIMVFSVFFLWSIYAYTLRNLDLLERFHVLPKNRSI--LLLEVTGLRLGMIGLLC 189

Query: 121 LVFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGHMTMAFFSAHGICFVIYWAATNNLSQV 180
           +VFLF P++RGS LL L+ +  E   +YH+WLGH+TMAFFS HG+C+V+ W     L ++
Sbjct: 190 MVFLFLPISRGSILLRLIDIPFEHATRYHVWLGHITMAFFSLHGLCYVVGWIIQGQLLEL 245

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BLAST of Lsi04G007990 vs. NCBI nr
Match: gi|659090166|ref|XP_008445871.1| (PREDICTED: ferric reduction oxidase 2-like [Cucumis melo])

HSP 1 Score: 299.3 bits (765), Expect = 4.8e-78
Identity = 145/180 (80.56%), Postives = 156/180 (86.67%), Query Frame = 1


Query: 1   TSLLMYTFPILFIAILGCVYIHLEQKCEDRNVETRKRKNHPGLTTWKQPAIMKGPLGIVS 60
           TSLLMYTFPILFIAILGCVY HLE+K  D+N+ETRK   H GL  WKQPAI+KGPLGIVS
Sbjct: 57  TSLLMYTFPILFIAILGCVYNHLEKKRSDQNIETRKGTKHHGLAMWKQPAIVKGPLGIVS 116

Query: 61  WTELALLAMFMTLLVWSFATYLHNSFVKITPQSAAMDGEKVWESRLGTAGFWLGIVGNIC 120
           WTEL LL MF+TLLVWSFATYLHNSF+           EK+WE RLG+AGFWLGIVGNIC
Sbjct: 117 WTELTLLTMFITLLVWSFATYLHNSFIS----------EKMWEIRLGSAGFWLGIVGNIC 176

Query: 121 LVFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGHMTMAFFSAHGICFVIYWAATNNLSQV 180
           LVFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGHMTMAFFSAHG+CFVIYW ATNN+SQ+
Sbjct: 177 LVFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGHMTMAFFSAHGVCFVIYWVATNNISQM 226

[Close]

BLAST of Lsi04G007990 vs. NCBI nr
Match: gi|449451731|ref|XP_004143614.1| (PREDICTED: ferric reduction oxidase 2-like [Cucumis sativus])

HSP 1 Score: 295.0 bits (754), Expect = 9.0e-77
Identity = 145/180 (80.56%), Postives = 155/180 (86.11%), Query Frame = 1


Query: 1   TSLLMYTFPILFIAILGCVYIHLEQKCEDRNVETRKRKNHPGLTTWKQPAIMKGPLGIVS 60
           TSLLMYTFPILFIAILGCVY HLE+K  D NV+TRK   H GL  WKQPAI+KGPLGIVS
Sbjct: 57  TSLLMYTFPILFIAILGCVYNHLEKKRSDPNVKTRKGTKHHGLAMWKQPAIVKGPLGIVS 116

Query: 61  WTELALLAMFMTLLVWSFATYLHNSFVKITPQSAAMDGEKVWESRLGTAGFWLGIVGNIC 120
           WTEL LL MF+ LLVWSFATYLHNSF+           EK+WE RLG+AGFWLGIVGNIC
Sbjct: 117 WTELTLLTMFIILLVWSFATYLHNSFIT----------EKMWEIRLGSAGFWLGIVGNIC 176

Query: 121 LVFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGHMTMAFFSAHGICFVIYWAATNNLSQV 180
           LVFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGHMTMAFFSAHGICF+IYWAATNN+SQ+
Sbjct: 177 LVFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGHMTMAFFSAHGICFLIYWAATNNISQM 226

[Close]

BLAST of Lsi04G007990 vs. NCBI nr
Match: gi|46577790|gb|AAT01415.1| (ferric reductase [Cucumis sativus])

HSP 1 Score: 295.0 bits (754), Expect = 9.0e-77
Identity = 145/180 (80.56%), Postives = 155/180 (86.11%), Query Frame = 1


Query: 1   TSLLMYTFPILFIAILGCVYIHLEQKCEDRNVETRKRKNHPGLTTWKQPAIMKGPLGIVS 60
           TSLLMYTFPILFIAILGCVY HLE+K  D NV+TRK   H GL  WKQPAI+KGPLGIVS
Sbjct: 57  TSLLMYTFPILFIAILGCVYNHLEKKRSDPNVKTRKGTKHHGLAMWKQPAIVKGPLGIVS 116

Query: 61  WTELALLAMFMTLLVWSFATYLHNSFVKITPQSAAMDGEKVWESRLGTAGFWLGIVGNIC 120
           WTEL LL MF+ LLVWSFATYLHNSF+           EK+WE RLG+AGFWLGIVGNIC
Sbjct: 117 WTELTLLTMFIILLVWSFATYLHNSFIT----------EKMWEIRLGSAGFWLGIVGNIC 176

Query: 121 LVFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGHMTMAFFSAHGICFVIYWAATNNLSQV 180
           LVFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGHMTMAFFSAHGICF+IYWAATNN+SQ+
Sbjct: 177 LVFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGHMTMAFFSAHGICFLIYWAATNNISQM 226

[Close]

BLAST of Lsi04G007990 vs. NCBI nr
Match: gi|700195276|gb|KGN50453.1| (hypothetical protein Csa_5G175770 [Cucumis sativus])

HSP 1 Score: 242.7 bits (618), Expect = 5.3e-61
Identity = 117/146 (80.14%), Postives = 125/146 (85.62%), Query Frame = 1


Query: 35  RKRKNHPGLTTWKQPAIMKGPLGIVSWTELALLAMFMTLLVWSFATYLHNSFVKITPQSA 94
           RK   H GL  WKQPAI+KGPLGIVSWTEL LL MF+ LLVWSFATYLHNSF+       
Sbjct: 14  RKGTKHHGLAMWKQPAIVKGPLGIVSWTELTLLTMFIILLVWSFATYLHNSFIT------ 73

Query: 95  AMDGEKVWESRLGTAGFWLGIVGNICLVFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGH 154
               EK+WE RLG+AGFWLGIVGNICLVFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGH
Sbjct: 74  ----EKMWEIRLGSAGFWLGIVGNICLVFLFFPVARGSSLLPLLGLTSEGCIKYHIWLGH 133

Query: 155 MTMAFFSAHGICFVIYWAATNNLSQV 181
           MTMAFFSAHGICF+IYWAATNN+SQ+
Sbjct: 134 MTMAFFSAHGICFLIYWAATNNISQM 149

[Close]

BLAST of Lsi04G007990 vs. NCBI nr
Match: gi|331271990|gb|AED02589.1| (ferric reductase [Lotus japonicus])

HSP 1 Score: 240.0 bits (611), Expect = 3.4e-60
Identity = 113/178 (63.48%), Postives = 137/178 (76.97%), Query Frame = 1


Query: 3   LLMYTFPILFIAILGCVYIHLEQKCEDRNVETRKRKNHPGLTTWKQPAIMKGPLGIVSWT 62
           +L +TFP+LFIA+LGCVY+H            +K  N P  T WK+P ++KGPLGIVS T
Sbjct: 69  ILTFTFPVLFIAVLGCVYLH----------TAKKSSNGPEATIWKRPVLVKGPLGIVSST 128

Query: 63  ELALLAMFMTLLVWSFATYLHNSFVKITPQSAAMDGEKVWESRLGTAGFWLGIVGNICLV 122
           ELA L MF+ LLVWSFATYLHN F KIT +SAA DGEKVWE +L  A   LG++GNICL 
Sbjct: 129 ELAFLLMFIALLVWSFATYLHNGFAKITQKSAAADGEKVWEEKLEXAALRLGLIGNICLA 188

Query: 123 FLFFPVARGSSLLPLLGLTSEGCIKYHIWLGHMTMAFFSAHGICFVIYWAATNNLSQV 181
           FL+FPVARGS++LPLLGLTSE CIKYHIWLGH+ M  F++HGIC++IYWAAT  +SQ+
Sbjct: 189 FLYFPVARGSTVLPLLGLTSESCIKYHIWLGHVVMTLFTSHGICYIIYWAATTQISQM 236

The following BLAST results are available for this feature:

BLAST of Lsi04G007990 vs. Swiss-Prot


Match Name	E-value	Identity	Description
FRO2_ARATH	1.2e-52	53.59	Ferric reduction oxidase 2 OS=Arabidopsis thaliana GN=FRO2 PE=1 SV=2	[more]
FRO1_ARATH	5.1e-48	49.43	Probable ferric reduction oxidase 1 OS=Arabidopsis thaliana GN=FRO1 PE=2 SV=1	[more]
FRO3_ARATH	1.4e-45	48.89	Ferric reduction oxidase 3, mitochondrial OS=Arabidopsis thaliana GN=FRO3 PE=2 S...	[more]
FRO4_ARATH	3.6e-33	41.34	Ferric reduction oxidase 4 OS=Arabidopsis thaliana GN=FRO4 PE=2 SV=1	[more]
FRO5_ARATH	3.7e-30	39.36	Ferric reduction oxidase 5 OS=Arabidopsis thaliana GN=FRO5 PE=2 SV=1	[more]

BLAST of Lsi04G007990 vs. TrEMBL


Match Name	E-value	Identity	Description
Q6PND7_CUCSA	6.3e-77	80.56	Ferric reductase OS=Cucumis sativus PE=2 SV=1	[more]
A0A0A0KLI0_CUCSA	3.7e-61	80.14	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G175770 PE=4 SV=1	[more]
F5BSM2_LOTJA	2.4e-60	63.48	Ferric reductase OS=Lotus japonicus GN=FRO1 PE=2 SV=1	[more]
F5BSM3_LOTJA	3.1e-60	64.20	Ferric reductase OS=Lotus japonicus GN=FRO1 PE=2 SV=1	[more]
W9SCD8_9ROSA	9.1e-60	60.89	Ferric reduction oxidase 2 OS=Morus notabilis GN=L484_021470 PE=4 SV=1	[more]

BLAST of Lsi04G007990 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G01580.1	6.7e-54	53.59	ferric reduction oxidase 2	[more]
AT1G01590.1	2.9e-49	49.43	ferric reduction oxidase 1	[more]
AT1G23020.2	7.9e-47	48.89	ferric reduction oxidase 3	[more]
AT5G23980.1	2.0e-34	41.34	ferric reduction oxidase 4	[more]
AT5G49730.1	5.9e-26	33.33	ferric reduction oxidase 6	[more]

BLAST of Lsi04G007990 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659090166\|ref\|XP_008445871.1\|	4.8e-78	80.56	PREDICTED: ferric reduction oxidase 2-like [Cucumis melo]	[more]
gi\|449451731\|ref\|XP_004143614.1\|	9.0e-77	80.56	PREDICTED: ferric reduction oxidase 2-like [Cucumis sativus]	[more]
gi\|46577790\|gb\|AAT01415.1\|	9.0e-77	80.56	ferric reductase [Cucumis sativus]	[more]
gi\|700195276\|gb\|KGN50453.1\|	5.3e-61	80.14	hypothetical protein Csa_5G175770 [Cucumis sativus]	[more]
gi\|331271990\|gb\|AED02589.1\|	3.4e-60	63.48	ferric reductase [Lotus japonicus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR013130	Fe3_Rdtase_TM_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0016174	NAD(P)H oxidase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G007990.1	Lsi04G007990.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013130	Ferric reductase transmembrane component-like domain	PFAM	PF01794	Ferric_reduct	coord: 113..177 score: 3.
None	No IPR available	PANTHER	PTHR11972	NADPH OXIDASE	coord: 1..188 score: 2.1
None	No IPR available	PANTHER	PTHR11972:SF41	FERRIC REDUCTION OXIDASE 2-RELATED	coord: 1..188 score: 2.1

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi04G007990	Csa5G175770	Cucumber (Chinese Long) v2	culsiB363
Lsi04G007990	CSPI05G08690	Wild cucumber (PI 183967)	cpilsiB366
Lsi04G007990	Cucsa.166120	Cucumber (Gy14) v1	cgylsiB276
Lsi04G007990	Cla020061	Watermelon (97103) v1	lsiwmB331
Lsi04G007990	MELO3C011744	Melon (DHL92) v3.5.1	lsimeB281
Lsi04G007990	ClCG02G014010	Watermelon (Charleston Gray)	lsiwcgB275
Lsi04G007990	MELO3C011744.2	Melon (DHL92) v3.6.1	lsimedB297
Lsi04G007990	Cla97C02G039740	Watermelon (97103) v2	lsiwmbB275
Lsi04G007990	Bhi07G000934	Wax gourd	lsiwgoB382
Lsi04G007990	Cp4.1LG01g10120	Cucurbita pepo (Zucchini)	cpelsiB358
Lsi04G007990	CsGy5G006070	Cucumber (Gy14) v2	cgyblsiB331
Lsi04G007990	Carg02057	Silver-seed gourd	carlsiB765
Lsi04G007990	CsaV3_5G006150	Cucumber (Chinese Long) v3	cuclsiB387

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi04G007990	Bottle gourd (USVL1VR-Ls)	lsilsiB004
Lsi04G007990	Bottle gourd (USVL1VR-Ls)	lsilsiB127
Lsi04G007990	Cucurbita maxima (Rimu)	cmalsiB676
Lsi04G007990	Cucurbita maxima (Rimu)	cmalsiB677
Lsi04G007990	Cucurbita moschata (Rifu)	cmolsiB669
Lsi04G007990	Cucurbita moschata (Rifu)	cmolsiB670
Lsi04G007990	Cucurbita pepo (Zucchini)	cpelsiB359
Lsi04G007990	Melon (DHL92) v3.6.1	lsimedB295
Lsi04G007990	Silver-seed gourd	carlsiB434
Lsi04G007990	Silver-seed gourd	carlsiB774