Lsi04G006860 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi04G006860
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionAlpha-ketoglutarate-dependent dioxygenase alkB
Locationchr04 : 6276437 .. 6278269 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAACCCTAAGCTTGATTATTCCTCTACCGTCCGTCGCCAGAATTCAGAAGCTCAATCTTACGCCGATCGATCATCTCACCGGCGACAGCGGATCCTGGTGGATAAAATGTACGGATCCGACCAAGGTATCGACGATTCCGAGCGCACCGCTTTCAGAAGAGCAGAAAAGAAATACAAATTGTACTACGACGACACCTACAAATCTTCGAAAAAGTATTCCTTCACAATTGCTCTTTCAGGTTCTATGTCATTCTATTGCCATTGAATTCCTTGCAAGAGACGATGTTTCAAACTCTCTTTTTTGCGATTTCTGTCAGGAAAAAACTACCGAAACAAGTCGATTTGTCTGAGGTTATCGATTTCAAGTGCATCCTCGAATGTTACAAACAAGATGGTGCACTTCCGCTGGGCGTGTACGCGACTAAGTGCGATCTCGATGGGCCAGTCTTCTGCTTGGAGAATCGTCCTGGTATTGCCGATTCTCCATTTTCGGCTCTGTTTCAAAGAAAAAGTTCCCAATTCAGCTGTTCTTTATGTGTTTAACATGTAGGGTTTTATTTTATTCCTGGAGCGTTGAGCTTACAAGAGCAATGCCAATGGATCAGGGAGAGTTTAACGAATTTCCCGCAGCCTCCTAACAGAACCAACCACAATGCTATTTATGGACCAATTAAAGACTTGTTCATTGCAGCAAAGGGAAAGAAAGTTTTAGTTGAAGATGATGAAATCTCTGATTTCAACGTTGATTCTGATGTTGAACCTTCTGTTAGCAATGGAAGTACTCATAACTGGAAGTTTGTGGAGGAGCATACTGTTTCATCCAGAAGAGGGTCGGCCTGCAAATCAATTCCTGCTTCAGTATTACTTCGAAAGTTGCGTTGGAGTACACTCGGCCTACAATTTGATTGGTCCAAGGTGAGTTCAACTATAAAACTCCATAGTTTGCTCGTATTATACCAATTTAAACTCTGTTTTGAATGTTTTGAATGGTTCCTATTTGAAAGTTGTATTTTATGAGACTATTCCAAAACGCTAAAACCATCATAGTATTGTCTCTGTTGCTATGTTTTGATATGTTATTTTCTGAATTATTCACCTTGTTATGGCGTAGCGAAGCTATGACATATCTCTGCCCCATAATAAGATACCCTCTGCCCTTTGTCAACTCGCCAAAAGAATGGCGGCAGCTGCAATGCCAACTGGGGAAGAATTCAAGCCTGAAGCTGCAATAGTTAATTATTTTGCTTCAGGTAACTAGAGAGAGTTAACAAAATTTTGCCATTGTCGAGGATTCTACATTGGAAACATTAGGACTCACAATGTTTATTAGATAGCTACTCCTCTCGGCTCTCGCCAATTGATTTTAAGATGAAATCCTTGTTAGTTAATAGCCATGACGCTGACACGTTATCGATATTTCCAGGTGACACGCTTGGTGGTCACCTAGATGACATGGAAGTGGACTGGAGCAAGCCAATTGTTAGCATGAGGTATCCTGCTAACTTATCCTCTTGCAATTGCATTTCTTTCTGATATTTAAAAGTACATGGGCTGATACTGATTCTGTTATCTATTATATCTGCTCGCCTCCACTTTGTCCAGTTTGGGTTGCAAAGCTATTTTTCTTTTGGGTGGTAAGTCGAGACAGGATCCACCCGTAGCCATGTTTCTTCGAAGCGGAGATGTTGTGCTAATGGCTGGAGAAGCAAGGGAATGTTTCCATGGTAATGTCGCCTTAATGTTCACTCTTACATCATATCAAAATGTATTTCTATATCAGATACCTGCACTTGCATTGTCCTTTTGTTTTGTTATCACCTTGGATATCTGA

mRNA sequence

CAAAACCCTAAGCTTGATTATTCCTCTACCGTCCGTCGCCAGAATTCAGAAGCTCAATCTTACGCCGATCGATCATCTCACCGGCGACAGCGGATCCTGGTGGATAAAATGTACGGATCCGACCAAGGTATCGACGATTCCGAGCGCACCGCTTTCAGAAGAGCAGAAAAGAAATACAAATTGTACTACGACGACACCTACAAATCTTCGAAAAAGAAAAAACTACCGAAACAAGTCGATTTGTCTGAGGTTATCGATTTCAAGTGCATCCTCGAATGTTACAAACAAGATGGTGCACTTCCGCTGGGCGTGTACGCGACTAAGTGCGATCTCGATGGGCCAGTCTTCTGCTTGGAGAATCGTCCTGGGTTTTATTTTATTCCTGGAGCGTTGAGCTTACAAGAGCAATGCCAATGGATCAGGGAGAGTTTAACGAATTTCCCGCAGCCTCCTAACAGAACCAACCACAATGCTATTTATGGACCAATTAAAGACTTGTTCATTGCAGCAAAGGGAAAGAAAGTTTTAGTTGAAGATGATGAAATCTCTGATTTCAACGTTGATTCTGATGTTGAACCTTCTGTTAGCAATGGAAGTACTCATAACTGGAAGTTTGTGGAGGAGCATACTGTTTCATCCAGAAGAGGGTCGGCCTGCAAATCAATTCCTGCTTCAGTATTACTTCGAAAGTTGCGTTGGAGTACACTCGGCCTACAATTTGATTGGTCCAAGCGAAGCTATGACATATCTCTGCCCCATAATAAGATACCCTCTGCCCTTTGTCAACTCGCCAAAAGAATGGCGGCAGCTGCAATGCCAACTGGGGAAGAATTCAAGCCTGAAGCTGCAATAGTTAATTATTTTGCTTCAGGTGACACGCTTGGTGGTCACCTAGATGACATGGAAGTGGACTGGAGCAAGCCAATTGTTAGCATGAGTTTGGGTTGCAAAGCTATTTTTCTTTTGGGTGGTAAGTCGAGACAGGATCCACCCGTAGCCATGTTTCTTCGAAGCGGAGATGTTGTGCTAATGGCTGGAGAAGCAAGGGAATGTTTCCATGGTAATGTCGCCTTAATGTTCACTCTTACATCATATCAAAATGTATTTCTATATCAGATACCTGCACTTGCATTGTCCTTTTGTTTTGTTATCACCTTGGATATCTGA

Coding sequence (CDS)

ATGTACGGATCCGACCAAGGTATCGACGATTCCGAGCGCACCGCTTTCAGAAGAGCAGAAAAGAAATACAAATTGTACTACGACGACACCTACAAATCTTCGAAAAAGAAAAAACTACCGAAACAAGTCGATTTGTCTGAGGTTATCGATTTCAAGTGCATCCTCGAATGTTACAAACAAGATGGTGCACTTCCGCTGGGCGTGTACGCGACTAAGTGCGATCTCGATGGGCCAGTCTTCTGCTTGGAGAATCGTCCTGGGTTTTATTTTATTCCTGGAGCGTTGAGCTTACAAGAGCAATGCCAATGGATCAGGGAGAGTTTAACGAATTTCCCGCAGCCTCCTAACAGAACCAACCACAATGCTATTTATGGACCAATTAAAGACTTGTTCATTGCAGCAAAGGGAAAGAAAGTTTTAGTTGAAGATGATGAAATCTCTGATTTCAACGTTGATTCTGATGTTGAACCTTCTGTTAGCAATGGAAGTACTCATAACTGGAAGTTTGTGGAGGAGCATACTGTTTCATCCAGAAGAGGGTCGGCCTGCAAATCAATTCCTGCTTCAGTATTACTTCGAAAGTTGCGTTGGAGTACACTCGGCCTACAATTTGATTGGTCCAAGCGAAGCTATGACATATCTCTGCCCCATAATAAGATACCCTCTGCCCTTTGTCAACTCGCCAAAAGAATGGCGGCAGCTGCAATGCCAACTGGGGAAGAATTCAAGCCTGAAGCTGCAATAGTTAATTATTTTGCTTCAGGTGACACGCTTGGTGGTCACCTAGATGACATGGAAGTGGACTGGAGCAAGCCAATTGTTAGCATGAGTTTGGGTTGCAAAGCTATTTTTCTTTTGGGTGGTAAGTCGAGACAGGATCCACCCGTAGCCATGTTTCTTCGAAGCGGAGATGTTGTGCTAATGGCTGGAGAAGCAAGGGAATGTTTCCATGGTAATGTCGCCTTAATGTTCACTCTTACATCATATCAAAATGTATTTCTATATCAGATACCTGCACTTGCATTGTCCTTTTGTTTTGTTATCACCTTGGATATCTGA

Protein sequence

MYGSDQGIDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILECYKQDGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRTNHNAIYGPIKDLFIAAKGKKVLVEDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSRRGSACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFHGNVALMFTLTSYQNVFLYQIPALALSFCFVITLDI
Homology
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BLAST of Lsi04G006860 vs. Swiss-Prot
Match: ALKBH_ARATH (Alpha-ketoglutarate-dependent dioxygenase alkB OS=Arabidopsis thaliana GN=At1g11780 PE=2 SV=2)

HSP 1 Score: 451.1 bits (1159), Expect = 1.1e-125
Identity = 219/318 (68.87%), Postives = 248/318 (77.99%), Query Frame = 1 

Query: 1   MYGSDQGIDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILECYKQ 60
           MY S    DD++RTAFRRAEKKYKLYY+   K S+KKKLPK +DLSE++DF  I + +  
Sbjct: 1   MYESANVSDDADRTAFRRAEKKYKLYYEQDSKFSRKKKLPKPIDLSELLDFNLISQNFNN 60

Query: 61  DGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRTNH 120
           DG LP G+  +K D   PVFC++NRPGFYFIP ALSL+EQC+WI+ESLT+FPQPPNRTNH
Sbjct: 61  DGVLPDGIRVSKVD-SSPVFCIDNRPGFYFIPDALSLKEQCKWIKESLTSFPQPPNRTNH 120

Query: 121 NAIYGPIKDLFIAAKGKKVLVEDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSRRG 180
           NAIYGPI DLF +AK  KVLV+DD                  + + WKF EE  +     
Sbjct: 121 NAIYGPIDDLFDSAKENKVLVQDDL-----------------TNNKWKFYEEVDIEKATR 180

Query: 181 SACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGE 240
           S+CKS+ ASVLLRKLRWSTLGLQFDWSKR+YD+SLPHN IP ALCQLAK  AA AMP GE
Sbjct: 181 SSCKSVSASVLLRKLRWSTLGLQFDWSKRNYDVSLPHNNIPDALCQLAKTHAAIAMPDGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EF+PE AIVNYF  GDTLGGHLDDME DWSKPIVSMSLGCKAIFLLGGKS+ DPP AM+L
Sbjct: 241 EFRPEGAIVNYFGIGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSKDDPPHAMYL 300

Query: 301 RSGDVVLMAGEARECFHG 319
           RSGDVVLMAGEARECFHG
Sbjct: 301 RSGDVVLMAGEARECFHG 300

[Close]
BLAST of Lsi04G006860 vs. Swiss-Prot
Match: ALKB_DICDI (Alpha-ketoglutarate-dependent dioxygenase alkB OS=Dictyostelium discoideum GN=alkB PE=2 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 3.1e-38
Identity = 105/335 (31.34%), Postives = 165/335 (49.25%), Query Frame = 1 

Query: 14  TAFRRAEKKYKLYYDDTYKSSKKKKLPKQ----VDLSEVIDF-----------KCILEC- 73
           T F R ++ ++       KS+  K +PK+    +D S V+DF           K I++C 
Sbjct: 36  TEFMRVQRLFR----QVTKSANGKNIPKEKREPIDYSPVLDFHNLENNTEENKKLIIDCT 95

Query: 74  ---------YKQDGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESL 133
                    + +D    L     K      V+ L+  PGFYFI    +  +Q +WI+ +L
Sbjct: 96  SNVTTHDFEFNRDTEFYLHPREWK------VYGLQGYPGFYFIKSPFTASQQKKWIKHAL 155

Query: 134 TNFPQPPNRTNHNAIYGPIKDLFIAAKGKKVLVEDDEIS-----DFNVDSDVEPSVSNGS 193
            ++  PPN  N    +GPIK+L+    G+K L+ ++  S     D  ++    P   NG 
Sbjct: 156 EDYADPPNNNNITLFHGPIKNLW--KNGEKELINEELKSQGKHDDDEIEQPTRPLDKNG- 215

Query: 194 THNWKFVEEHTVSSRRGSACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSA 253
                   E   + R+           LL KL WSTLG Q+ W+ R Y     + + P  
Sbjct: 216 --------EPLPTYRQ-----------LLDKLAWSTLGYQYQWTPRLYSEEF-YEEFPDD 275

Query: 254 LCQLAKRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAI 313
           L +L +++A A     + +  EAA VN+++    +GGHLDD E +  KPI+S+S G  A+
Sbjct: 276 LQELVQKIAIATK--FDPYVAEAATVNFYSEDSIMGGHLDDAEQEMEKPIISISFGSTAV 335

Query: 314 FLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFHG 319
           FL+G ++R   PV +F+RSGD+V+M G +R C+HG
Sbjct: 336 FLMGAETRDIAPVPLFIRSGDIVIMGGRSRYCYHG 335

[Close]
BLAST of Lsi04G006860 vs. Swiss-Prot
Match: ALKB1_HUMAN (DNA demethylase ALKBH1 OS=Homo sapiens GN=ALKBH1 PE=1 SV=2)

HSP 1 Score: 111.7 bits (278), Expect = 1.7e-23
Identity = 67/167 (40.12%), Postives = 94/167 (56.29%), Query Frame = 1 

Query: 156 EPSVSNGSTHNWK-----FVEEHTVSSRRGSACKSIPASVLLRKLRWSTLGLQFDWSKRS 215
           +P+V N   H  K       E+     R   A K  P S LL KLRW T+G  ++W  + 
Sbjct: 125 KPNVCNLDKHMSKEETQDLWEQSKEFLRYKEATKRRPRS-LLEKLRWVTVGYHYNWDSKK 184

Query: 216 YDISLPHNKIPSALCQLAKRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEVDWS 275
           Y     +   PS L  L++++AAA     E+F+ EA I+NY+    TLG H+D  E+D S
Sbjct: 185 YSAD-HYTPFPSDLGFLSEQVAAAC--GFEDFRAEAGILNYYRLDSTLGIHVDRSELDHS 244

Query: 276 KPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFH 318
           KP++S S G  AIFLLGG  R + P AMF+ SGD+++M+G +R   H
Sbjct: 245 KPLLSFSFGQSAIFLLGGLQRDEAPTAMFMHSGDIMIMSGFSRLLNH 287

[Close]
BLAST of Lsi04G006860 vs. Swiss-Prot
Match: ALKB1_MOUSE (DNA demethylase ALKBH1 OS=Mus musculus GN=Alkbh1 PE=1 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.8e-22
Identity = 65/167 (38.92%), Postives = 91/167 (54.49%), Query Frame = 1 

Query: 156 EPSVSNGSTHNWK-----FVEEHTVSSRRGSACKSIPASVLLRKLRWSTLGLQFDWSKRS 215
           +P+V N   H  K       E+     R     K  P S LL +LRW TLG  ++W  + 
Sbjct: 125 KPNVCNLDKHMTKEETQGLWEQSKEVLRSKEVTKRRPRS-LLERLRWVTLGYHYNWDSKK 184

Query: 216 YDISLPHNKIPSALCQLAKRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEVDWS 275
           Y     +   PS L  L++++A A    G  F+ EA I+NY+    TLG H+D  E+D S
Sbjct: 185 YSAD-HYTPFPSDLAFLSEQVATACGFQG--FQAEAGILNYYRLDSTLGIHVDRSELDHS 244

Query: 276 KPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFH 318
           KP++S S G  AIFLLGG  R + P AMF+ SGD+++M+G +R   H
Sbjct: 245 KPLLSFSFGQSAIFLLGGLKRDEAPTAMFMHSGDIMVMSGFSRLLNH 287

[Close]
BLAST of Lsi04G006860 vs. Swiss-Prot
Match: ALKBH_SCHPO (Alpha-ketoglutarate-dependent dioxygenase abh1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=abh1 PE=2 SV=3)

HSP 1 Score: 107.5 bits (267), Expect = 3.1e-22
Identity = 50/125 (40.00%), Postives = 77/125 (61.60%), Query Frame = 1 

Query: 193 RKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGEEFKPEAAIVNYF 252
           +KLRW TLG Q+DW+ + Y         P  L    +++   +      +K EAAIVN++
Sbjct: 138 KKLRWVTLGEQYDWTTKEYPDPSKSPGFPKDLGDFVEKVVKESTDF-LHWKAEAAIVNFY 197

Query: 253 ASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEA 312
           + GDTL  H+D+ E D + P++S+S+G   I+L+G +SR + P A+ L SGDVV+M G +
Sbjct: 198 SPGDTLSAHIDESEEDLTLPLISLSMGLDCIYLIGTESRSEKPSALRLHSGDVVIMTGTS 257

Query: 313 RECFH 318
           R+ FH
Sbjct: 258 RKAFH 261

[Close]
BLAST of Lsi04G006860 vs. TrEMBL
Match: A0A0A0KS00_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G589370 PE=4 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 2.4e-170
Identity = 292/318 (91.82%), Postives = 304/318 (95.60%), Query Frame = 1 

Query: 1   MYGSDQGIDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILECYKQ 60
           MYGSD+G DDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPK VDLSEVIDFK ILE Y+Q
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYQQ 60

Query: 61  DGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRTNH 120
           DG+LP+GV AT CDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESL  FPQPPNRTNH
Sbjct: 61  DGSLPVGVNATTCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLMEFPQPPNRTNH 120

Query: 121 NAIYGPIKDLFIAAKGKKVLVEDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSRRG 180
           NAIYGPI+DLFIAAK  KVLVE DEISDF +DSDVEPS+SNG+THNWKFVEE+TVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWKFVEENTVSSRRG 180

Query: 181 SACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGE 240
           +A KSIPASVLLRKLRWSTLGLQFDWSKRSY+ISLPHNKIPSALCQLAKRMAAAAMPTGE
Sbjct: 181 TAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLAKRMAAAAMPTGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EFKPEAAIVNYFASGDTLGGHLDDME DWSKPIVSMSLGCKAIFLLGGKSRQDPP+AMFL
Sbjct: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPIAMFL 300

Query: 301 RSGDVVLMAGEARECFHG 319
           RSGDVVLMAGEARECFHG
Sbjct: 301 RSGDVVLMAGEARECFHG 318

[Close]
BLAST of Lsi04G006860 vs. TrEMBL
Match: M5XD22_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007688mg PE=4 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 2.7e-137
Identity = 244/327 (74.62%), Postives = 268/327 (81.96%), Query Frame = 1 

Query: 1   MYGSDQGI--DDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILECY 60
           MYGS+ G   +D ERTAFRRAEKKYKLYY+DTYKSSKKK+ PK VDLSEV+DF  ILE Y
Sbjct: 1   MYGSESGTVPEDLERTAFRRAEKKYKLYYEDTYKSSKKKRKPKPVDLSEVLDFNSILESY 60

Query: 61  KQDGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRT 120
            Q+  LP GV   +CD D PVF LENRPGFYFIPGAL ++EQCQWI+ESLT+FPQPPNRT
Sbjct: 61  YQNVELPHGVVPLRCDFDRPVFSLENRPGFYFIPGALRVKEQCQWIKESLTSFPQPPNRT 120

Query: 121 NHNAIYGPIKDLFIAAKGKKVLVEDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSR 180
           NHNA YGPI DLFIAA  +KVLV D        +S+ +PSVSNG  H WKF EE  VSS+
Sbjct: 121 NHNAFYGPINDLFIAANERKVLVADG-----GSNSECDPSVSNGGVHGWKFFEEREVSSK 180

Query: 181 RGSACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPT 240
            GS CKS+ ASVLLRKLRWSTLGLQFDWSKR+YD+SLPH KIP  LCQLAKR+A  AMP 
Sbjct: 181 -GSTCKSVSASVLLRKLRWSTLGLQFDWSKRNYDVSLPHKKIPDTLCQLAKRLATPAMPL 240

Query: 241 GEEFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAM 300
           GEEF+PE AIVNYF  GD LGGHLDDME DWSKPIVSMSLGCKAIFLLGGK R+DPP+AM
Sbjct: 241 GEEFQPEGAIVNYFGPGDMLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKHREDPPIAM 300

Query: 301 FLRSGDVVLMAGEARECFHGNVALMFT 326
           FLRSGDVVLMAGEARECFHG V  +FT
Sbjct: 301 FLRSGDVVLMAGEARECFHG-VPRIFT 320

[Close]
BLAST of Lsi04G006860 vs. TrEMBL
Match: A0A067JS77_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_17964 PE=4 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 1.0e-136
Identity = 244/325 (75.08%), Postives = 270/325 (83.08%), Query Frame = 1 

Query: 1   MYGSDQGIDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILECYKQ 60
           MYGSD+  D+SERTA RRAEKKYKLYYD   KSSKKKK PKQVDLSEV+DFK IL+ Y Q
Sbjct: 1   MYGSDRVTDESERTALRRAEKKYKLYYDQDSKSSKKKKRPKQVDLSEVLDFKSILDSYNQ 60

Query: 61  DGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRTNH 120
           +G L  G+ A  C  D PVFCLE+RPGFYFIPGALS++EQC WIRESL NFPQPPNRTNH
Sbjct: 61  NGELSYGILAVHCGFDRPVFCLESRPGFYFIPGALSIEEQCLWIRESLMNFPQPPNRTNH 120

Query: 121 NAIYGPIKDLFIAAKGKKVLVEDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSRRG 180
           NAIYGPI DLFIAAKG+KVL+ED+  S  N+D   +  V NG    WKF EE+  SSR G
Sbjct: 121 NAIYGPIHDLFIAAKGRKVLMEDEN-SLLNLDGS-DACVDNGDACRWKFYEEYVASSR-G 180

Query: 181 SACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGE 240
             CKS+ AS LLR+LRWSTLGLQFDWSKR+Y++SLPHNKIP+ L QLAKR+AA AMP GE
Sbjct: 181 KTCKSVSASALLRRLRWSTLGLQFDWSKRNYNVSLPHNKIPNDLQQLAKRLAAPAMPMGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EF+PEAAIVNYF  GDTLGGHLDDME DWSKPIVSMSLGCKAIFLLGGK R+DPP+AMFL
Sbjct: 241 EFRPEAAIVNYFGLGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKCREDPPLAMFL 300

Query: 301 RSGDVVLMAGEARECFHGNVALMFT 326
           RSGDVVLMAGEARECFHG V  +FT
Sbjct: 301 RSGDVVLMAGEARECFHG-VPRIFT 321

[Close]
BLAST of Lsi04G006860 vs. TrEMBL
Match: A0A0B0N645_GOSAR (Uncharacterized protein OS=Gossypium arboreum GN=F383_14429 PE=4 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 3.8e-136
Identity = 244/343 (71.14%), Postives = 274/343 (79.88%), Query Frame = 1 

Query: 1   MYGSDQGIDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILECYKQ 60
           MYGSD+  DD+ERTAFR+ EKKYKLYY+++ KS+KKKK PKQVDLSEV+DFK I E + Q
Sbjct: 1   MYGSDRAGDDAERTAFRKTEKKYKLYYENS-KSTKKKKQPKQVDLSEVLDFKSISESFNQ 60

Query: 61  DGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRTNH 120
           +  LP GV    C  D P+F LEN PGFYF PGAL+L EQC WIRESLTNFPQPPNRTNH
Sbjct: 61  NVELPPGVVHVNCGFDRPIFGLENSPGFYFFPGALTLDEQCTWIRESLTNFPQPPNRTNH 120

Query: 121 NAIYGPIKDLFIAAKGKKVLVEDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSRRG 180
           N IYGPI +LF+AAK  KV +E+ E SD  +DS+   SVSNG +H WKF EE    SR G
Sbjct: 121 NVIYGPISNLFVAAKEGKVFIEE-ETSDNGLDSEPSASVSNGDSHRWKFYEEDIARSR-G 180

Query: 181 SACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGE 240
            ACK++PASVLLRKLRWSTLGLQFDWS R+Y +SLPHNKIP+ALCQLAKRMA  AMP GE
Sbjct: 181 KACKTVPASVLLRKLRWSTLGLQFDWSNRNYAVSLPHNKIPNALCQLAKRMAEPAMPIGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EF+PEAAIVNYF SGDTLGGHLDDME DWSKPIVSMS GCKAIFLLGGKSRQD P+AMFL
Sbjct: 241 EFRPEAAIVNYFGSGDTLGGHLDDMEADWSKPIVSMSFGCKAIFLLGGKSRQDEPLAMFL 300

Query: 301 RSGDVVLMAGEARECFHGNVALMFTLTSYQNVFLYQIPALALS 344
           RSGDVVLMAGEARECFHG ++L        N FL+ +  L  S
Sbjct: 301 RSGDVVLMAGEARECFHGWISL-------PNSFLFHVMVLEFS 333

[Close]
BLAST of Lsi04G006860 vs. TrEMBL
Match: A0A0D2V6K8_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_012G175900 PE=4 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 2.5e-135
Identity = 241/325 (74.15%), Postives = 270/325 (83.08%), Query Frame = 1 

Query: 1   MYGSDQGIDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILECYKQ 60
           MYGSD+  DD+ERTAFR+ EK YKLYY+++ KS+KKKK PKQVDLSEV+DFK I E + Q
Sbjct: 1   MYGSDRAGDDAERTAFRKTEKTYKLYYENS-KSTKKKKQPKQVDLSEVLDFKSISESFNQ 60

Query: 61  DGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRTNH 120
           +G LP GV    C  D P+F LENRPGFYFIPGAL+L EQC WIRESLTNFPQPPNRTNH
Sbjct: 61  NGELPPGVVHVICGFDRPIFGLENRPGFYFIPGALTLDEQCTWIRESLTNFPQPPNRTNH 120

Query: 121 NAIYGPIKDLFIAAKGKKVLVEDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSRRG 180
           N IYGPI +LF+AAK  KV VE +E SD  +DS+   SVSNG +H WKF EE  ++  RG
Sbjct: 121 NVIYGPISNLFVAAKEGKVFVE-EERSDNGLDSEPSASVSNGDSHRWKFYEE-DIARSRG 180

Query: 181 SACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGE 240
             CK++PASVLLRKLRWSTLGLQFDWS R+Y +SLPHNKIP+ALC+LAKRMA  AMP GE
Sbjct: 181 KTCKTVPASVLLRKLRWSTLGLQFDWSNRNYAVSLPHNKIPNALCRLAKRMAEPAMPIGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EF+PEAAIVNYF SGDTLGGHLDDME DWSKPIVSMS GCKAIFLLGGKSRQD P+AMFL
Sbjct: 241 EFRPEAAIVNYFGSGDTLGGHLDDMEADWSKPIVSMSFGCKAIFLLGGKSRQDEPLAMFL 300

Query: 301 RSGDVVLMAGEARECFHGNVALMFT 326
           RSGDVVLMAGEARECFHG V  +FT
Sbjct: 301 RSGDVVLMAGEARECFHG-VPRIFT 321

[Close]
BLAST of Lsi04G006860 vs. TAIR10
Match: AT1G11780.1 (AT1G11780.1 oxidoreductase, 2OG-Fe(II) oxygenase family protein)

HSP 1 Score: 451.1 bits (1159), Expect = 6.5e-127
Identity = 219/318 (68.87%), Postives = 248/318 (77.99%), Query Frame = 1 

Query: 1   MYGSDQGIDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILECYKQ 60
           MY S    DD++RTAFRRAEKKYKLYY+   K S+KKKLPK +DLSE++DF  I + +  
Sbjct: 1   MYESANVSDDADRTAFRRAEKKYKLYYEQDSKFSRKKKLPKPIDLSELLDFNLISQNFNN 60

Query: 61  DGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRTNH 120
           DG LP G+  +K D   PVFC++NRPGFYFIP ALSL+EQC+WI+ESLT+FPQPPNRTNH
Sbjct: 61  DGVLPDGIRVSKVD-SSPVFCIDNRPGFYFIPDALSLKEQCKWIKESLTSFPQPPNRTNH 120

Query: 121 NAIYGPIKDLFIAAKGKKVLVEDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSRRG 180
           NAIYGPI DLF +AK  KVLV+DD                  + + WKF EE  +     
Sbjct: 121 NAIYGPIDDLFDSAKENKVLVQDDL-----------------TNNKWKFYEEVDIEKATR 180

Query: 181 SACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGE 240
           S+CKS+ ASVLLRKLRWSTLGLQFDWSKR+YD+SLPHN IP ALCQLAK  AA AMP GE
Sbjct: 181 SSCKSVSASVLLRKLRWSTLGLQFDWSKRNYDVSLPHNNIPDALCQLAKTHAAIAMPDGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EF+PE AIVNYF  GDTLGGHLDDME DWSKPIVSMSLGCKAIFLLGGKS+ DPP AM+L
Sbjct: 241 EFRPEGAIVNYFGIGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSKDDPPHAMYL 300

Query: 301 RSGDVVLMAGEARECFHG 319
           RSGDVVLMAGEARECFHG
Sbjct: 301 RSGDVVLMAGEARECFHG 300

[Close]
BLAST of Lsi04G006860 vs. TAIR10
Match: AT3G14160.1 (AT3G14160.1 2-oxoglutarate-dependent dioxygenase family protein)

HSP 1 Score: 60.8 bits (146), Expect = 1.9e-09
Identity = 32/79 (40.51%), Postives = 45/79 (56.96%), Query Frame = 1 

Query: 244 PEAAIVNYFASGDTLGGHLDDMEVDWS----KPIVSMSLGCKAIFLLGGKSRQDPPVAMF 303
           P+  IVN+++S   LG H D  E + S     P+VS S+G  A FL G +  +D    + 
Sbjct: 346 PDICIVNFYSSTGRLGLHQDKDESENSIRKGLPVVSFSIGDSAEFLYGDQRDEDKAETLT 405

Query: 304 LRSGDVVLMAGEARECFHG 319
           L SGDV+L  G +R+ FHG
Sbjct: 406 LESGDVLLFGGRSRKVFHG 424

[Close]
BLAST of Lsi04G006860 vs. TAIR10
Match: AT5G01780.2 (AT5G01780.2 2-oxoglutarate-dependent dioxygenase family protein)

HSP 1 Score: 58.2 bits (139), Expect = 1.2e-08
Identity = 32/79 (40.51%), Postives = 43/79 (54.43%), Query Frame = 1 

Query: 244 PEAAIVNYFASGDTLGGHLDDMEVDWS----KPIVSMSLGCKAIFLLGGKSRQDPPVAMF 303
           P+  IVN+++    LG H D  E + S     PIVS S+G  A FL G K   +    + 
Sbjct: 333 PDICIVNFYSETGRLGLHQDRDESEESIARGLPIVSFSIGDSAEFLYGEKRDVEEAQGVI 392

Query: 304 LRSGDVVLMAGEARECFHG 319
           L SGDV++  GE+R  FHG
Sbjct: 393 LESGDVLIFGGESRMIFHG 411

[Close]
BLAST of Lsi04G006860 vs. TAIR10
Match: AT3G14140.1 (AT3G14140.1 2-oxoglutarate-dependent dioxygenase family protein)

HSP 1 Score: 48.5 bits (114), Expect = 9.7e-06
Identity = 48/171 (28.07%), Postives = 68/171 (39.77%), Query Frame = 1 

Query: 192 LRKLRWSTLGLQFDWSKRSY------DISLPHNKIPSALCQLAKR--------MAAAAMP 251
           L  L+   LG  +D   R Y      D S+P  +IP    QL ++        +A  +  
Sbjct: 281 LLHLKMMCLGKNWDCQTRRYGEIRPIDGSVPP-RIPVEFSQLVEKAIKESKSLVATNSNE 340

Query: 252 T--GEEFK---PEAAIVNYFASGDTLGGH----LDDMEVDWSK----------------- 311
           T  G+E     P+  +VN++ S   LG H     D    D+ K                 
Sbjct: 341 TKGGDEIPLLLPDICVVNFYTSTGKLGLHQVSVYDKTSFDFLKYKGGYLNTDKGESKKSL 400

Query: 312 ----PIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFHG 319
               PIVS S+G  A FL G +   D    + L SGDV++    +R  FHG
Sbjct: 401 RKGLPIVSFSIGDSAEFLYGDQKDVDKADTLILESGDVLIFGERSRNVFHG 450

[Close]
BLAST of Lsi04G006860 vs. NCBI nr
Match: gi|659090466|ref|XP_008446031.1| (PREDICTED: LOW QUALITY PROTEIN: alpha-ketoglutarate-dependent dioxygenase alkB [Cucumis melo])

HSP 1 Score: 609.8 bits (1571), Expect = 3.1e-171
Identity = 294/318 (92.45%), Postives = 305/318 (95.91%), Query Frame = 1 

Query: 1   MYGSDQGIDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILECYKQ 60
           MYGSD+G DDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPK VDLSEVIDFK ILE YKQ
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYKQ 60

Query: 61  DGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRTNH 120
           DG+LP+GV AT CDLD PVFCLENRPGFYFIPGALSLQEQCQWIRESL +FPQPPNRTNH
Sbjct: 61  DGSLPVGVKATTCDLDRPVFCLENRPGFYFIPGALSLQEQCQWIRESLMDFPQPPNRTNH 120

Query: 121 NAIYGPIKDLFIAAKGKKVLVEDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSRRG 180
           NAIYGPI+DLFIAAK KKVLVE DEISDFN+DSDVEPS+SNGSTHNWKFVEE+TVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKKVLVEHDEISDFNLDSDVEPSISNGSTHNWKFVEENTVSSRRG 180

Query: 181 SACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGE 240
           +ACKSI ASVLLRKLRWSTLGLQFDWSKRSY+ISLPHNKIPSALCQLAKRMAAAAMPTGE
Sbjct: 181 TACKSISASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLAKRMAAAAMPTGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EFKPEAAIVNYFASGDTLGGHLDDME DWSKPIVSMSLGCKA FLLGGKSRQDPP+AMFL
Sbjct: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAXFLLGGKSRQDPPIAMFL 300

Query: 301 RSGDVVLMAGEARECFHG 319
           RSGDVVLMAGEARECFHG
Sbjct: 301 RSGDVVLMAGEARECFHG 318

[Close]
BLAST of Lsi04G006860 vs. NCBI nr
Match: gi|449435087|ref|XP_004135327.1| (PREDICTED: alpha-ketoglutarate-dependent dioxygenase alkB [Cucumis sativus])

HSP 1 Score: 606.3 bits (1562), Expect = 3.4e-170
Identity = 292/318 (91.82%), Postives = 304/318 (95.60%), Query Frame = 1 

Query: 1   MYGSDQGIDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILECYKQ 60
           MYGSD+G DDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPK VDLSEVIDFK ILE Y+Q
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYQQ 60

Query: 61  DGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRTNH 120
           DG+LP+GV AT CDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESL  FPQPPNRTNH
Sbjct: 61  DGSLPVGVNATTCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLMEFPQPPNRTNH 120

Query: 121 NAIYGPIKDLFIAAKGKKVLVEDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSRRG 180
           NAIYGPI+DLFIAAK  KVLVE DEISDF +DSDVEPS+SNG+THNWKFVEE+TVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWKFVEENTVSSRRG 180

Query: 181 SACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGE 240
           +A KSIPASVLLRKLRWSTLGLQFDWSKRSY+ISLPHNKIPSALCQLAKRMAAAAMPTGE
Sbjct: 181 TAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLAKRMAAAAMPTGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EFKPEAAIVNYFASGDTLGGHLDDME DWSKPIVSMSLGCKAIFLLGGKSRQDPP+AMFL
Sbjct: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPIAMFL 300

Query: 301 RSGDVVLMAGEARECFHG 319
           RSGDVVLMAGEARECFHG
Sbjct: 301 RSGDVVLMAGEARECFHG 318

[Close]
BLAST of Lsi04G006860 vs. NCBI nr
Match: gi|596130719|ref|XP_007222157.1| (hypothetical protein PRUPE_ppa007688mg [Prunus persica])

HSP 1 Score: 496.5 bits (1277), Expect = 3.8e-137
Identity = 244/327 (74.62%), Postives = 268/327 (81.96%), Query Frame = 1 

Query: 1   MYGSDQGI--DDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILECY 60
           MYGS+ G   +D ERTAFRRAEKKYKLYY+DTYKSSKKK+ PK VDLSEV+DF  ILE Y
Sbjct: 1   MYGSESGTVPEDLERTAFRRAEKKYKLYYEDTYKSSKKKRKPKPVDLSEVLDFNSILESY 60

Query: 61  KQDGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRT 120
            Q+  LP GV   +CD D PVF LENRPGFYFIPGAL ++EQCQWI+ESLT+FPQPPNRT
Sbjct: 61  YQNVELPHGVVPLRCDFDRPVFSLENRPGFYFIPGALRVKEQCQWIKESLTSFPQPPNRT 120

Query: 121 NHNAIYGPIKDLFIAAKGKKVLVEDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSR 180
           NHNA YGPI DLFIAA  +KVLV D        +S+ +PSVSNG  H WKF EE  VSS+
Sbjct: 121 NHNAFYGPINDLFIAANERKVLVADG-----GSNSECDPSVSNGGVHGWKFFEEREVSSK 180

Query: 181 RGSACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPT 240
            GS CKS+ ASVLLRKLRWSTLGLQFDWSKR+YD+SLPH KIP  LCQLAKR+A  AMP 
Sbjct: 181 -GSTCKSVSASVLLRKLRWSTLGLQFDWSKRNYDVSLPHKKIPDTLCQLAKRLATPAMPL 240

Query: 241 GEEFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAM 300
           GEEF+PE AIVNYF  GD LGGHLDDME DWSKPIVSMSLGCKAIFLLGGK R+DPP+AM
Sbjct: 241 GEEFQPEGAIVNYFGPGDMLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKHREDPPIAM 300

Query: 301 FLRSGDVVLMAGEARECFHGNVALMFT 326
           FLRSGDVVLMAGEARECFHG V  +FT
Sbjct: 301 FLRSGDVVLMAGEARECFHG-VPRIFT 320

[Close]
BLAST of Lsi04G006860 vs. NCBI nr
Match: gi|645230937|ref|XP_008222162.1| (PREDICTED: alpha-ketoglutarate-dependent dioxygenase alkB [Prunus mume])

HSP 1 Score: 496.1 bits (1276), Expect = 5.0e-137
Identity = 244/327 (74.62%), Postives = 268/327 (81.96%), Query Frame = 1 

Query: 1   MYGSDQGI--DDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILECY 60
           MYGS+ G   +D ERTAFRRAEKKYKLYY+DTYKSSKKK+ PK VDLSEV+DF  ILE Y
Sbjct: 1   MYGSESGSVPEDLERTAFRRAEKKYKLYYEDTYKSSKKKRKPKPVDLSEVLDFNSILESY 60

Query: 61  KQDGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRT 120
            Q+  LP GV   +C  D PVF LENRPGFYFIPGALS++EQCQWI+ESLT+FPQPPNRT
Sbjct: 61  YQNVELPHGVVPLRCGFDRPVFSLENRPGFYFIPGALSVKEQCQWIKESLTSFPQPPNRT 120

Query: 121 NHNAIYGPIKDLFIAAKGKKVLVEDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSR 180
           NHNA YGPI DLFIAA  +KVLV D        +S+ +PSVSNG  H WKF EEH VS +
Sbjct: 121 NHNAFYGPINDLFIAANERKVLVADG-----GSNSECDPSVSNGGVHGWKFFEEHEVSLK 180

Query: 181 RGSACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPT 240
            GS CKS+ ASVLLRKLRWSTLGLQFDWSKR+YD+SLPH KIP  LCQLAKR+A  AMP 
Sbjct: 181 -GSTCKSVSASVLLRKLRWSTLGLQFDWSKRNYDVSLPHKKIPDTLCQLAKRLATPAMPL 240

Query: 241 GEEFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAM 300
           GEEF+PE AIVNYF  GD LGGHLDDME DWSKPIVSMSLGCKAIFLLGGK R+DPP+AM
Sbjct: 241 GEEFQPEGAIVNYFGPGDMLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKHREDPPIAM 300

Query: 301 FLRSGDVVLMAGEARECFHGNVALMFT 326
           FLRSGDVVLMAGEARECFHG V  +FT
Sbjct: 301 FLRSGDVVLMAGEARECFHG-VPRIFT 320

[Close]
BLAST of Lsi04G006860 vs. NCBI nr
Match: gi|802724306|ref|XP_012085682.1| (PREDICTED: alpha-ketoglutarate-dependent dioxygenase alkB isoform X1 [Jatropha curcas])

HSP 1 Score: 494.6 bits (1272), Expect = 1.4e-136
Identity = 244/325 (75.08%), Postives = 270/325 (83.08%), Query Frame = 1 

Query: 1   MYGSDQGIDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILECYKQ 60
           MYGSD+  D+SERTA RRAEKKYKLYYD   KSSKKKK PKQVDLSEV+DFK IL+ Y Q
Sbjct: 1   MYGSDRVTDESERTALRRAEKKYKLYYDQDSKSSKKKKRPKQVDLSEVLDFKSILDSYNQ 60

Query: 61  DGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRTNH 120
           +G L  G+ A  C  D PVFCLE+RPGFYFIPGALS++EQC WIRESL NFPQPPNRTNH
Sbjct: 61  NGELSYGILAVHCGFDRPVFCLESRPGFYFIPGALSIEEQCLWIRESLMNFPQPPNRTNH 120

Query: 121 NAIYGPIKDLFIAAKGKKVLVEDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSRRG 180
           NAIYGPI DLFIAAKG+KVL+ED+  S  N+D   +  V NG    WKF EE+  SSR G
Sbjct: 121 NAIYGPIHDLFIAAKGRKVLMEDEN-SLLNLDGS-DACVDNGDACRWKFYEEYVASSR-G 180

Query: 181 SACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGE 240
             CKS+ AS LLR+LRWSTLGLQFDWSKR+Y++SLPHNKIP+ L QLAKR+AA AMP GE
Sbjct: 181 KTCKSVSASALLRRLRWSTLGLQFDWSKRNYNVSLPHNKIPNDLQQLAKRLAAPAMPMGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EF+PEAAIVNYF  GDTLGGHLDDME DWSKPIVSMSLGCKAIFLLGGK R+DPP+AMFL
Sbjct: 241 EFRPEAAIVNYFGLGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKCREDPPLAMFL 300

Query: 301 RSGDVVLMAGEARECFHGNVALMFT 326
           RSGDVVLMAGEARECFHG V  +FT
Sbjct: 301 RSGDVVLMAGEARECFHG-VPRIFT 321

The following BLAST results are available for this feature:
BLAST of Lsi04G006860 vs. Swiss-Prot
Match NameE-valueIdentityDescription
ALKBH_ARATH1.1e-12568.87Alpha-ketoglutarate-dependent dioxygenase alkB OS=Arabidopsis thaliana GN=At1g11... [more]
ALKB_DICDI3.1e-3831.34Alpha-ketoglutarate-dependent dioxygenase alkB OS=Dictyostelium discoideum GN=al... [more]
ALKB1_HUMAN1.7e-2340.12DNA demethylase ALKBH1 OS=Homo sapiens GN=ALKBH1 PE=1 SV=2[more]
ALKB1_MOUSE1.8e-2238.92DNA demethylase ALKBH1 OS=Mus musculus GN=Alkbh1 PE=1 SV=1[more]
ALKBH_SCHPO3.1e-2240.00Alpha-ketoglutarate-dependent dioxygenase abh1 OS=Schizosaccharomyces pombe (str... [more]
BLAST of Lsi04G006860 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KS00_CUCSA2.4e-17091.82Uncharacterized protein OS=Cucumis sativus GN=Csa_5G589370 PE=4 SV=1[more]
M5XD22_PRUPE2.7e-13774.62Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007688mg PE=4 SV=1[more]
A0A067JS77_JATCU1.0e-13675.08Uncharacterized protein OS=Jatropha curcas GN=JCGZ_17964 PE=4 SV=1[more]
A0A0B0N645_GOSAR3.8e-13671.14Uncharacterized protein OS=Gossypium arboreum GN=F383_14429 PE=4 SV=1[more]
A0A0D2V6K8_GOSRA2.5e-13574.15Uncharacterized protein OS=Gossypium raimondii GN=B456_012G175900 PE=4 SV=1[more]
BLAST of Lsi04G006860 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G11780.16.5e-12768.87 oxidoreductase, 2OG-Fe(II) oxygenase family protein[more]
AT3G14160.11.9e-0940.51 2-oxoglutarate-dependent dioxygenase family protein[more]
AT5G01780.21.2e-0840.51 2-oxoglutarate-dependent dioxygenase family protein[more]
AT3G14140.19.7e-0628.07 2-oxoglutarate-dependent dioxygenase family protein[more]
BLAST of Lsi04G006860 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659090466|ref|XP_008446031.1|3.1e-17192.45PREDICTED: LOW QUALITY PROTEIN: alpha-ketoglutarate-dependent dioxygenase alkB [... [more]
gi|449435087|ref|XP_004135327.1|3.4e-17091.82PREDICTED: alpha-ketoglutarate-dependent dioxygenase alkB [Cucumis sativus][more]
gi|596130719|ref|XP_007222157.1|3.8e-13774.62hypothetical protein PRUPE_ppa007688mg [Prunus persica][more]
gi|645230937|ref|XP_008222162.1|5.0e-13774.62PREDICTED: alpha-ketoglutarate-dependent dioxygenase alkB [Prunus mume][more]
gi|802724306|ref|XP_012085682.1|1.4e-13675.08PREDICTED: alpha-ketoglutarate-dependent dioxygenase alkB isoform X1 [Jatropha c... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO:0006281DNA repair
Vocabulary: INTERPRO
TermDefinition
IPR027450AlkB-like
IPR004574Alkb
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006281 DNA repair
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function GO:0051213 dioxygenase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G006860.1Lsi04G006860.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004574Alkylated DNA repair protein AlkBPANTHERPTHR16557ALKYLATED DNA REPAIR PROTEIN ALKB-RELATEDcoord: 167..318
score: 3.1E-136coord: 1..141
score: 3.1E
IPR027450Alpha-ketoglutarate-dependent dioxygenase AlkB-likeGENE3DG3DSA:2.60.120.590coord: 180..318
score: 9.5E-58coord: 81..121
score: 9.5
IPR027450Alpha-ketoglutarate-dependent dioxygenase AlkB-likePFAMPF135322OG-FeII_Oxy_2coord: 188..318
score: 5.3
NoneNo IPR availablePANTHERPTHR16557:SF2ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 1coord: 167..318
score: 3.1E-136coord: 1..141
score: 3.1E
NoneNo IPR availableunknownSSF51197Clavaminate synthase-likecoord: 86..121
score: 2.47E-39coord: 180..318
score: 2.47