Lsi04G006130 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi04G006130
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionCytochrome P450
Locationchr04 : 5606092 .. 5606643 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGAGCTAGTTAAGAATCCACGGGTAATGAAGAAGCTACAAGATGAGATCAGAAGCTGCATAAAAGAAGATCTAGTGAAGGAGAACGACCTTGAAAAACTTCAATATCTAAAAGTGGTGGTGAAAGAAGTTCTAAGGTTACACCCGCCTGCCCCACTTCTACTCCCAAGAGAAACCATGTCTCAATTTAAGCTCGATAGTTACGACATTGATCCCAAAACTCATCTTCATGTCAATGTATGGGCGATTGGGCGAGATCCAAATTACTGGACTCATCCAGAAGAATTCCTACCTGAGAGATTTATGGGAAGCAATATTGATTACAAAGGGCAGAATTTTGAGTTATTACCATTTGGAGCTGGTCGAAGAATATGTCCTGGTATGAACATGGGGATTGTTACGATGGAGTTGGCTTTGGCTAATCTATTGCTACAATTTAACTGGCAGTTACCGAATGGGATTAAAGAAGAAGATGTGGACATGGAAGAGGATGTTGGTCTAACCGTGGCAAAGAAATCACCTCTTAAACTCATTCCAATTGCCTATTGA

mRNA sequence

ATGGCAGAGCTAGTTAAGAATCCACGGGTAATGAAGAAGCTACAAGATGAGATCAGAAGCTGCATAAAAGAAGATCTAGTGAAGGAGAACGACCTTGAAAAACTTCAATATCTAAAAGTGGTGGTGAAAGAAGTTCTAAGGTTACACCCGCCTGCCCCACTTCTACTCCCAAGAGAAACCATGTCTCAATTTAAGCTCGATAGTTACGACATTGATCCCAAAACTCATCTTCATGTCAATGTATGGGCGATTGGGCGAGATCCAAATTACTGGACTCATCCAGAAGAATTCCTACCTGAGAGATTTATGGGAAGCAATATTGATTACAAAGGGCAGAATTTTGAGTTATTACCATTTGGAGCTGGTCGAAGAATATGTCCTGGTATGAACATGGGGATTGTTACGATGGAGTTGGCTTTGGCTAATCTATTGCTACAATTTAACTGGCAGTTACCGAATGGGATTAAAGAAGAAGATGTGGACATGGAAGAGGATGTTGGTCTAACCGTGGCAAAGAAATCACCTCTTAAACTCATTCCAATTGCCTATTGA

Coding sequence (CDS)

ATGGCAGAGCTAGTTAAGAATCCACGGGTAATGAAGAAGCTACAAGATGAGATCAGAAGCTGCATAAAAGAAGATCTAGTGAAGGAGAACGACCTTGAAAAACTTCAATATCTAAAAGTGGTGGTGAAAGAAGTTCTAAGGTTACACCCGCCTGCCCCACTTCTACTCCCAAGAGAAACCATGTCTCAATTTAAGCTCGATAGTTACGACATTGATCCCAAAACTCATCTTCATGTCAATGTATGGGCGATTGGGCGAGATCCAAATTACTGGACTCATCCAGAAGAATTCCTACCTGAGAGATTTATGGGAAGCAATATTGATTACAAAGGGCAGAATTTTGAGTTATTACCATTTGGAGCTGGTCGAAGAATATGTCCTGGTATGAACATGGGGATTGTTACGATGGAGTTGGCTTTGGCTAATCTATTGCTACAATTTAACTGGCAGTTACCGAATGGGATTAAAGAAGAAGATGTGGACATGGAAGAGGATGTTGGTCTAACCGTGGCAAAGAAATCACCTCTTAAACTCATTCCAATTGCCTATTGA

Protein sequence

MAELVKNPRVMKKLQDEIRSCIKEDLVKENDLEKLQYLKVVVKEVLRLHPPAPLLLPRETMSQFKLDSYDIDPKTHLHVNVWAIGRDPNYWTHPEEFLPERFMGSNIDYKGQNFELLPFGAGRRICPGMNMGIVTMELALANLLLQFNWQLPNGIKEEDVDMEEDVGLTVAKKSPLKLIPIAY
BLAST of Lsi04G006130 vs. Swiss-Prot
Match: C71BO_ARATH (Cytochrome P450 71B24 OS=Arabidopsis thaliana GN=CYP71B24 PE=2 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 1.6e-62
Identity = 116/186 (62.37%), Postives = 148/186 (79.57%), Query Frame = 1

Query: 1   MAELVKNPRVMKKLQDEIRSCI---KEDLVKENDLEKLQYLKVVVKEVLRLHPPAPLLLP 60
           MAELVKNPRVMKK+QDEIR+CI   + + ++E+D++KLQYLK+VVKE LRLHP APLLLP
Sbjct: 314 MAELVKNPRVMKKVQDEIRTCIGIKQNEKIEEDDVDKLQYLKLVVKETLRLHPAAPLLLP 373

Query: 61  RETMSQFKLDSYDIDPKTHLHVNVWAIGRDPNYWTHPEEFLPERFMGSNIDYKGQNFELL 120
           RETMSQ K+  Y+I  KT L VNVW+IGRDP +W +PEEF PERF+   IDYKG +FE+L
Sbjct: 374 RETMSQIKIQGYNIPSKTILLVNVWSIGRDPKHWKNPEEFNPERFIDCPIDYKGNSFEML 433

Query: 121 PFGAGRRICPGMNMGIVTMELALANLLLQFNWQLPNGIKEEDVDMEEDVGLTVAKKSPLK 180
           PFG+GRRICPG+   I T+EL L NLL  F+W+LP   +++D+DMEE   +T+ KK PLK
Sbjct: 434 PFGSGRRICPGIAFAIATVELGLLNLLYHFDWRLPE--EDKDLDMEEAGDVTIIKKVPLK 493

Query: 181 LIPIAY 184
           L+P+ +
Sbjct: 494 LVPVLH 497

BLAST of Lsi04G006130 vs. Swiss-Prot
Match: C71BQ_ARATH (Cytochrome P450 71B26 OS=Arabidopsis thaliana GN=CYP71B26 PE=2 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 6.1e-62
Identity = 114/184 (61.96%), Postives = 143/184 (77.72%), Query Frame = 1

Query: 1   MAELVKNPRVMKKLQDEIRSCIK-EDLVKENDLEKLQYLKVVVKEVLRLHPPAPLLLPRE 60
           MAEL KNPRVMKK+Q EIR+ IK ++ +  +D +KL+YLK+V+KE  RLHPP PLLLPR+
Sbjct: 314 MAELAKNPRVMKKVQAEIRNQIKNKERISFDDTDKLEYLKMVIKETWRLHPPTPLLLPRD 373

Query: 61  TMSQFKLDSYDIDPKTHLHVNVWAIGRDPNYWTHPEEFLPERFMGSNIDYKGQNFELLPF 120
            +++F+++ Y I  KT LHVNVWAIGRDP+ W  PE FLPERF  SNID KGQNFELL F
Sbjct: 374 VITEFEINGYTIPAKTRLHVNVWAIGRDPDTWKDPEMFLPERFNDSNIDAKGQNFELLSF 433

Query: 121 GAGRRICPGMNMGIVTMELALANLLLQFNWQLPNGIKEEDVDMEEDVGLTVAKKSPLKLI 180
           G+GRRICPG+ MG   +E  LAN+L  F+W+LP G+  ED+DMEE  GLTV+KKS L L+
Sbjct: 434 GSGRRICPGLYMGTTMVEFGLANMLYHFDWKLPEGMVVEDIDMEEAPGLTVSKKSELVLV 493

Query: 181 PIAY 184
           P+ Y
Sbjct: 494 PVKY 497

BLAST of Lsi04G006130 vs. Swiss-Prot
Match: C71B9_ARATH (Cytochrome P450 71B9 OS=Arabidopsis thaliana GN=CYP71B9 PE=2 SV=3)

HSP 1 Score: 238.0 bits (606), Expect = 7.9e-62
Identity = 111/184 (60.33%), Postives = 143/184 (77.72%), Query Frame = 1

Query: 1   MAELVKNPRVMKKLQDEIRS-CIKEDLVKENDLEKLQYLKVVVKEVLRLHPPAPLLLPRE 60
           MAEL++NPRVMKK+Q EIR+  I + ++  +D++ L YLK+V+KE  RLHPP PLLLPRE
Sbjct: 314 MAELIRNPRVMKKVQSEIRNQMINKSVITLDDIDHLPYLKMVIKETWRLHPPVPLLLPRE 373

Query: 61  TMSQFKLDSYDIDPKTHLHVNVWAIGRDPNYWTHPEEFLPERFMGSNIDYKGQNFELLPF 120
            MS+F+++ Y I PKT L+VNVWAIGRDP+ W   + F PERFM +NID KGQNFELLPF
Sbjct: 374 VMSEFEINGYKIQPKTLLYVNVWAIGRDPDSWKDADMFYPERFMDNNIDAKGQNFELLPF 433

Query: 121 GAGRRICPGMNMGIVTMELALANLLLQFNWQLPNGIKEEDVDMEEDVGLTVAKKSPLKLI 180
           G+GRRICPGM MG   +E  LAN+L QF+W++P+G+  ED+DMEE  GL V KK+ L L+
Sbjct: 434 GSGRRICPGMYMGTTMVEFGLANMLYQFDWEVPDGMVVEDIDMEESPGLAVGKKNELLLV 493

Query: 181 PIAY 184
           P+ Y
Sbjct: 494 PVKY 497

BLAST of Lsi04G006130 vs. Swiss-Prot
Match: C71B4_ARATH (Cytochrome P450 71B4 OS=Arabidopsis thaliana GN=CYP71B4 PE=2 SV=2)

HSP 1 Score: 233.0 bits (593), Expect = 2.6e-60
Identity = 116/184 (63.04%), Postives = 140/184 (76.09%), Query Frame = 1

Query: 1   MAELVKNPRVMKKLQDEIRSCI---KEDLVKENDLEKLQYLKVVVKEVLRLHPPAPLLLP 60
           MAELV+NPRVMKK QDEIR+CI   +E  + E DL+KLQYLK+VVKE LRLHP APLLLP
Sbjct: 318 MAELVRNPRVMKKAQDEIRTCIGIKQEGRIMEEDLDKLQYLKLVVKETLRLHPAAPLLLP 377

Query: 61  RETMSQFKLDSYDIDPKTHLHVNVWAIGRDPNYWTHPEEFLPERFMGSNIDYKGQNFELL 120
           RETM+  K+  YDI  K  L VN W+IGRDP  W +PEEF PERF+   +DYKG +FELL
Sbjct: 378 RETMADIKIQGYDIPQKRALLVNAWSIGRDPESWKNPEEFNPERFIDCPVDYKGHSFELL 437

Query: 121 PFGAGRRICPGMNMGIVTMELALANLLLQFNWQLPNGIKEEDVDMEEDVGLTVAKKSPLK 180
           PFG+GRRICPG+ M I T+EL L NLL  F+W +P   K++D+DMEE   LTV KK PL+
Sbjct: 438 PFGSGRRICPGIAMAIATIELGLLNLLYFFDWNMPE--KKKDMDMEEAGDLTVDKKVPLE 497

Query: 181 LIPI 182
           L+P+
Sbjct: 498 LLPV 499

BLAST of Lsi04G006130 vs. Swiss-Prot
Match: C71BV_ARATH (Cytochrome P450 71B34 OS=Arabidopsis thaliana GN=CYP71B34 PE=2 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 3.3e-60
Identity = 112/184 (60.87%), Postives = 140/184 (76.09%), Query Frame = 1

Query: 1   MAELVKNPRVMKKLQDEIRSCIK-EDLVKENDLEKLQYLKVVVKEVLRLHPPAPLLLPRE 60
           MAEL KNPRVMKK+Q EIRS IK ++ +  +D +KL+YLK+V+KE  RLHP  PLL+PRE
Sbjct: 314 MAELAKNPRVMKKVQSEIRSQIKNKERISFDDTDKLEYLKMVIKETWRLHPTTPLLIPRE 373

Query: 61  TMSQFKLDSYDIDPKTHLHVNVWAIGRDPNYWTHPEEFLPERFMGSNIDYKGQNFELLPF 120
            MS+F+++ Y I  KT LHVNVWAIGRDP+ W  PE FLPERF  +NID KGQ+FELLPF
Sbjct: 374 AMSEFEINGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLPERFTDNNIDAKGQHFELLPF 433

Query: 121 GAGRRICPGMNMGIVTMELALANLLLQFNWQLPNGIKEEDVDMEEDVGLTVAKKSPLKLI 180
           G GRR+CP + MG   +E  LANLL  F+W+LP G+K +D+DMEE  GLTV KK+ L L+
Sbjct: 434 GGGRRMCPAVYMGTTMVEFGLANLLYHFDWKLPEGMKVDDIDMEEAPGLTVNKKNELILV 493

Query: 181 PIAY 184
           P  +
Sbjct: 494 PTKF 497

BLAST of Lsi04G006130 vs. TrEMBL
Match: W9RKJ2_9ROSA (Cytochrome P450 OS=Morus notabilis GN=L484_027660 PE=3 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 5.2e-68
Identity = 125/184 (67.93%), Postives = 151/184 (82.07%), Query Frame = 1

Query: 1   MAELVKNPRVMKKLQDEIRSCIKED-LVKENDLEKLQYLKVVVKEVLRLHPPAPLLLPRE 60
           MAELV+NPRVM+K QDEIRSC+K+  LV E+D+ +L YL++V+KE LRLHP  PLLLPRE
Sbjct: 322 MAELVRNPRVMRKAQDEIRSCVKKGKLVSESDIHQLHYLRMVIKETLRLHPAIPLLLPRE 381

Query: 61  TMSQFKLDSYDIDPKTHLHVNVWAIGRDPNYWTHPEEFLPERFMGSNIDYKGQNFELLPF 120
            MSQFKL  YD+ PKT L VNVWAIGRDPNYW +PEEF PERF   +ID+KGQ+FE LPF
Sbjct: 382 AMSQFKLFGYDVYPKTLLQVNVWAIGRDPNYWHNPEEFFPERFSDGSIDFKGQHFEFLPF 441

Query: 121 GAGRRICPGMNMGIVTMELALANLLLQFNWQLPNGIKEEDVDMEEDVGLTVAKKSPLKLI 180
           GAGRR CPG++MGI  +EL LANLL  F+W+LP+G+KE D+DM+E+ GLT  KK PLKLI
Sbjct: 442 GAGRRTCPGIHMGIAMVELTLANLLCCFDWKLPDGMKETDIDMDEESGLTTHKKFPLKLI 501

Query: 181 PIAY 184
           P+ Y
Sbjct: 502 PVRY 505

BLAST of Lsi04G006130 vs. TrEMBL
Match: F6GVN1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0137g00540 PE=3 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 1.7e-66
Identity = 124/184 (67.39%), Postives = 149/184 (80.98%), Query Frame = 1

Query: 1   MAELVKNPRVMKKLQDEIRSCI-KEDLVKENDLEKLQYLKVVVKEVLRLHPPAPLLLPRE 60
           M EL +NP +MKK Q E+RS I  +  V E+D+++L YLKVVVKE LRLHPPAPLLLPRE
Sbjct: 320 MTELARNPGIMKKAQAEVRSSIGNKGKVTESDVDQLHYLKVVVKETLRLHPPAPLLLPRE 379

Query: 61  TMSQFKLDSYDIDPKTHLHVNVWAIGRDPNYWTHPEEFLPERFMGSNIDYKGQNFELLPF 120
           TMS F+++ Y I PKT +HVNVWAIGRDPN W +PEEFLPERFM +++D++GQ+FELLPF
Sbjct: 380 TMSHFEINGYHIYPKTQVHVNVWAIGRDPNLWKNPEEFLPERFMDNSVDFRGQHFELLPF 439

Query: 121 GAGRRICPGMNMGIVTMELALANLLLQFNWQLPNGIKEEDVDMEEDVGLTVAKKSPLKLI 180
           GAGRRICPGM M I T+ELALANLL +FNW LPNGI+E D+ MEE  GLTV KK  L L+
Sbjct: 440 GAGRRICPGMYMAIATVELALANLLYRFNWNLPNGIREADISMEEAAGLTVRKKFALNLV 499

Query: 181 PIAY 184
           PI +
Sbjct: 500 PILH 503

BLAST of Lsi04G006130 vs. TrEMBL
Match: W9S1E6_9ROSA (Cytochrome P450 OS=Morus notabilis GN=L484_027664 PE=3 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 2.8e-66
Identity = 123/184 (66.85%), Postives = 152/184 (82.61%), Query Frame = 1

Query: 1   MAELVKNPRVMKKLQDEIRSCIKE-DLVKENDLEKLQYLKVVVKEVLRLHPPAPLLLPRE 60
           MAEL +NPRVM+K QDEIR+ +K+ +LV E+++ +L YLK+VVKE +RLHP APLLLPRE
Sbjct: 314 MAELARNPRVMRKAQDEIRTRVKKGELVSESEIHQLDYLKMVVKETMRLHPAAPLLLPRE 373

Query: 61  TMSQFKLDSYDIDPKTHLHVNVWAIGRDPNYWTHPEEFLPERFMGSNIDYKGQNFELLPF 120
           TMSQFKL  YD+ PKT L VNVWAIGRDPNYW +PEEF+PERF   +IDYKGQ+FE LPF
Sbjct: 374 TMSQFKLFGYDVYPKTLLQVNVWAIGRDPNYWDNPEEFIPERFADGSIDYKGQHFEFLPF 433

Query: 121 GAGRRICPGMNMGIVTMELALANLLLQFNWQLPNGIKEEDVDMEEDVGLTVAKKSPLKLI 180
           GAGRR CPG+ MGI  +EL LANLL  F+W+LP+G++E D+DM+E  GL + KK PLKL+
Sbjct: 434 GAGRRGCPGIYMGIAMVELTLANLLCCFDWKLPDGMEETDIDMDEASGLAIHKKFPLKLV 493

Query: 181 PIAY 184
           PI+Y
Sbjct: 494 PISY 497

BLAST of Lsi04G006130 vs. TrEMBL
Match: F6GVM9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0137g00560 PE=3 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 4.8e-66
Identity = 122/184 (66.30%), Postives = 148/184 (80.43%), Query Frame = 1

Query: 1   MAELVKNPRVMKKLQDEIRSCI-KEDLVKENDLEKLQYLKVVVKEVLRLHPPAPLLLPRE 60
           M EL +NPR+MKK Q E+R+ I  +  V E D+++L YLK+VVKE LRLHPPAPLLLPRE
Sbjct: 320 MTELARNPRIMKKAQAEVRNSIGNKGKVTEGDVDQLHYLKMVVKETLRLHPPAPLLLPRE 379

Query: 61  TMSQFKLDSYDIDPKTHLHVNVWAIGRDPNYWTHPEEFLPERFMGSNIDYKGQNFELLPF 120
           TMS F+++ Y   PKT +HVNVWAIGRDPN W +PEEFLPERFM +++D++GQ+FELLPF
Sbjct: 380 TMSHFEINGYHFYPKTQVHVNVWAIGRDPNLWKNPEEFLPERFMDNSVDFRGQHFELLPF 439

Query: 121 GAGRRICPGMNMGIVTMELALANLLLQFNWQLPNGIKEEDVDMEEDVGLTVAKKSPLKLI 180
           GAGRRICPGM M I T+ELALANLL +FNW LPNG+KE D+ MEE  GLTV KK  L L+
Sbjct: 440 GAGRRICPGMYMAIATVELALANLLYRFNWNLPNGMKETDISMEEAAGLTVRKKFALNLV 499

Query: 181 PIAY 184
           PI +
Sbjct: 500 PILH 503

BLAST of Lsi04G006130 vs. TrEMBL
Match: A5AY94_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_023587 PE=3 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 9.1e-65
Identity = 120/184 (65.22%), Postives = 149/184 (80.98%), Query Frame = 1

Query: 1   MAELVKNPRVMKKLQDEIRSCI-KEDLVKENDLEKLQYLKVVVKEVLRLHPPAPLLLPRE 60
           M EL +NPR+MKK Q E+R+ I  +  V E D+++L YLK+VVKE LRLHPPAPLL+PRE
Sbjct: 320 MTELARNPRIMKKAQVEVRNSIGNKGKVTEGDVDQLHYLKMVVKETLRLHPPAPLLVPRE 379

Query: 61  TMSQFKLDSYDIDPKTHLHVNVWAIGRDPNYWTHPEEFLPERFMGSNIDYKGQNFELLPF 120
           TMS F+++ Y I PKT + VNVWAIGRDPN W +PEEFLPERFM +++D++GQ+FELLPF
Sbjct: 380 TMSHFEINGYHIYPKTQVXVNVWAIGRDPNLWKNPEEFLPERFMDNSVDFRGQHFELLPF 439

Query: 121 GAGRRICPGMNMGIVTMELALANLLLQFNWQLPNGIKEEDVDMEEDVGLTVAKKSPLKLI 180
           GAGRRICPGM M I T+ELALANLL +FNW LPNG++E D++MEE  GLTV KK  L L+
Sbjct: 440 GAGRRICPGMYMAIATVELALANLLYRFNWNLPNGMREADINMEEAAGLTVRKKFALNLV 499

Query: 181 PIAY 184
           PI +
Sbjct: 500 PILH 503

BLAST of Lsi04G006130 vs. TAIR10
Match: AT3G26230.1 (AT3G26230.1 cytochrome P450, family 71, subfamily B, polypeptide 24)

HSP 1 Score: 240.4 bits (612), Expect = 9.0e-64
Identity = 116/186 (62.37%), Postives = 148/186 (79.57%), Query Frame = 1

Query: 1   MAELVKNPRVMKKLQDEIRSCI---KEDLVKENDLEKLQYLKVVVKEVLRLHPPAPLLLP 60
           MAELVKNPRVMKK+QDEIR+CI   + + ++E+D++KLQYLK+VVKE LRLHP APLLLP
Sbjct: 314 MAELVKNPRVMKKVQDEIRTCIGIKQNEKIEEDDVDKLQYLKLVVKETLRLHPAAPLLLP 373

Query: 61  RETMSQFKLDSYDIDPKTHLHVNVWAIGRDPNYWTHPEEFLPERFMGSNIDYKGQNFELL 120
           RETMSQ K+  Y+I  KT L VNVW+IGRDP +W +PEEF PERF+   IDYKG +FE+L
Sbjct: 374 RETMSQIKIQGYNIPSKTILLVNVWSIGRDPKHWKNPEEFNPERFIDCPIDYKGNSFEML 433

Query: 121 PFGAGRRICPGMNMGIVTMELALANLLLQFNWQLPNGIKEEDVDMEEDVGLTVAKKSPLK 180
           PFG+GRRICPG+   I T+EL L NLL  F+W+LP   +++D+DMEE   +T+ KK PLK
Sbjct: 434 PFGSGRRICPGIAFAIATVELGLLNLLYHFDWRLPE--EDKDLDMEEAGDVTIIKKVPLK 493

Query: 181 LIPIAY 184
           L+P+ +
Sbjct: 494 LVPVLH 497

BLAST of Lsi04G006130 vs. TAIR10
Match: AT3G26290.1 (AT3G26290.1 cytochrome P450, family 71, subfamily B, polypeptide 26)

HSP 1 Score: 238.4 bits (607), Expect = 3.4e-63
Identity = 114/184 (61.96%), Postives = 143/184 (77.72%), Query Frame = 1

Query: 1   MAELVKNPRVMKKLQDEIRSCIK-EDLVKENDLEKLQYLKVVVKEVLRLHPPAPLLLPRE 60
           MAEL KNPRVMKK+Q EIR+ IK ++ +  +D +KL+YLK+V+KE  RLHPP PLLLPR+
Sbjct: 314 MAELAKNPRVMKKVQAEIRNQIKNKERISFDDTDKLEYLKMVIKETWRLHPPTPLLLPRD 373

Query: 61  TMSQFKLDSYDIDPKTHLHVNVWAIGRDPNYWTHPEEFLPERFMGSNIDYKGQNFELLPF 120
            +++F+++ Y I  KT LHVNVWAIGRDP+ W  PE FLPERF  SNID KGQNFELL F
Sbjct: 374 VITEFEINGYTIPAKTRLHVNVWAIGRDPDTWKDPEMFLPERFNDSNIDAKGQNFELLSF 433

Query: 121 GAGRRICPGMNMGIVTMELALANLLLQFNWQLPNGIKEEDVDMEEDVGLTVAKKSPLKLI 180
           G+GRRICPG+ MG   +E  LAN+L  F+W+LP G+  ED+DMEE  GLTV+KKS L L+
Sbjct: 434 GSGRRICPGLYMGTTMVEFGLANMLYHFDWKLPEGMVVEDIDMEEAPGLTVSKKSELVLV 493

Query: 181 PIAY 184
           P+ Y
Sbjct: 494 PVKY 497

BLAST of Lsi04G006130 vs. TAIR10
Match: AT2G02580.1 (AT2G02580.1 cytochrome P450, family 71, subfamily B, polypeptide 9)

HSP 1 Score: 238.0 bits (606), Expect = 4.5e-63
Identity = 111/184 (60.33%), Postives = 143/184 (77.72%), Query Frame = 1

Query: 1   MAELVKNPRVMKKLQDEIRS-CIKEDLVKENDLEKLQYLKVVVKEVLRLHPPAPLLLPRE 60
           MAEL++NPRVMKK+Q EIR+  I + ++  +D++ L YLK+V+KE  RLHPP PLLLPRE
Sbjct: 314 MAELIRNPRVMKKVQSEIRNQMINKSVITLDDIDHLPYLKMVIKETWRLHPPVPLLLPRE 373

Query: 61  TMSQFKLDSYDIDPKTHLHVNVWAIGRDPNYWTHPEEFLPERFMGSNIDYKGQNFELLPF 120
            MS+F+++ Y I PKT L+VNVWAIGRDP+ W   + F PERFM +NID KGQNFELLPF
Sbjct: 374 VMSEFEINGYKIQPKTLLYVNVWAIGRDPDSWKDADMFYPERFMDNNIDAKGQNFELLPF 433

Query: 121 GAGRRICPGMNMGIVTMELALANLLLQFNWQLPNGIKEEDVDMEEDVGLTVAKKSPLKLI 180
           G+GRRICPGM MG   +E  LAN+L QF+W++P+G+  ED+DMEE  GL V KK+ L L+
Sbjct: 434 GSGRRICPGMYMGTTMVEFGLANMLYQFDWEVPDGMVVEDIDMEESPGLAVGKKNELLLV 493

Query: 181 PIAY 184
           P+ Y
Sbjct: 494 PVKY 497

BLAST of Lsi04G006130 vs. TAIR10
Match: AT3G26280.1 (AT3G26280.1 cytochrome P450, family 71, subfamily B, polypeptide 4)

HSP 1 Score: 233.0 bits (593), Expect = 1.4e-61
Identity = 116/184 (63.04%), Postives = 140/184 (76.09%), Query Frame = 1

Query: 1   MAELVKNPRVMKKLQDEIRSCI---KEDLVKENDLEKLQYLKVVVKEVLRLHPPAPLLLP 60
           MAELV+NPRVMKK QDEIR+CI   +E  + E DL+KLQYLK+VVKE LRLHP APLLLP
Sbjct: 318 MAELVRNPRVMKKAQDEIRTCIGIKQEGRIMEEDLDKLQYLKLVVKETLRLHPAAPLLLP 377

Query: 61  RETMSQFKLDSYDIDPKTHLHVNVWAIGRDPNYWTHPEEFLPERFMGSNIDYKGQNFELL 120
           RETM+  K+  YDI  K  L VN W+IGRDP  W +PEEF PERF+   +DYKG +FELL
Sbjct: 378 RETMADIKIQGYDIPQKRALLVNAWSIGRDPESWKNPEEFNPERFIDCPVDYKGHSFELL 437

Query: 121 PFGAGRRICPGMNMGIVTMELALANLLLQFNWQLPNGIKEEDVDMEEDVGLTVAKKSPLK 180
           PFG+GRRICPG+ M I T+EL L NLL  F+W +P   K++D+DMEE   LTV KK PL+
Sbjct: 438 PFGSGRRICPGIAMAIATIELGLLNLLYFFDWNMPE--KKKDMDMEEAGDLTVDKKVPLE 497

Query: 181 LIPI 182
           L+P+
Sbjct: 498 LLPV 499

BLAST of Lsi04G006130 vs. TAIR10
Match: AT3G26300.1 (AT3G26300.1 cytochrome P450, family 71, subfamily B, polypeptide 34)

HSP 1 Score: 232.6 bits (592), Expect = 1.9e-61
Identity = 112/184 (60.87%), Postives = 140/184 (76.09%), Query Frame = 1

Query: 1   MAELVKNPRVMKKLQDEIRSCIK-EDLVKENDLEKLQYLKVVVKEVLRLHPPAPLLLPRE 60
           MAEL KNPRVMKK+Q EIRS IK ++ +  +D +KL+YLK+V+KE  RLHP  PLL+PRE
Sbjct: 314 MAELAKNPRVMKKVQSEIRSQIKNKERISFDDTDKLEYLKMVIKETWRLHPTTPLLIPRE 373

Query: 61  TMSQFKLDSYDIDPKTHLHVNVWAIGRDPNYWTHPEEFLPERFMGSNIDYKGQNFELLPF 120
            MS+F+++ Y I  KT LHVNVWAIGRDP+ W  PE FLPERF  +NID KGQ+FELLPF
Sbjct: 374 AMSEFEINGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLPERFTDNNIDAKGQHFELLPF 433

Query: 121 GAGRRICPGMNMGIVTMELALANLLLQFNWQLPNGIKEEDVDMEEDVGLTVAKKSPLKLI 180
           G GRR+CP + MG   +E  LANLL  F+W+LP G+K +D+DMEE  GLTV KK+ L L+
Sbjct: 434 GGGRRMCPAVYMGTTMVEFGLANLLYHFDWKLPEGMKVDDIDMEEAPGLTVNKKNELILV 493

Query: 181 PIAY 184
           P  +
Sbjct: 494 PTKF 497

BLAST of Lsi04G006130 vs. NCBI nr
Match: gi|659092302|ref|XP_008447001.1| (PREDICTED: cytochrome P450 71B34-like [Cucumis melo])

HSP 1 Score: 307.8 bits (787), Expect = 1.3e-80
Identity = 144/183 (78.69%), Postives = 165/183 (90.16%), Query Frame = 1

Query: 1   MAELVKNPRVMKKLQDEIRSCIKEDLVKENDLEKLQYLKVVVKEVLRLHPPAPLLLPRET 60
           MAEL+KNPRVMKKLQDEIRS IKED VKE+DLEKLQYLK+VVKEVLRLHPP PLLLPRE+
Sbjct: 324 MAELIKNPRVMKKLQDEIRSTIKEDRVKESDLEKLQYLKMVVKEVLRLHPPVPLLLPRES 383

Query: 61  MSQFKLDSYDIDPKTHLHVNVWAIGRDPNYWTHPEEFLPERFMGSNIDYKGQNFELLPFG 120
           MS FKL+ YDI PKTH+HVN WAIGRDP  W HP +F+PERF+ +NIDYKGQ+FEL+PFG
Sbjct: 384 MSHFKLNGYDIHPKTHIHVNAWAIGRDPESWKHPLQFIPERFIENNIDYKGQHFELIPFG 443

Query: 121 AGRRICPGMNMGIVTMELALANLLLQFNWQLPNGIKEEDVDMEEDVGLTVAKKSPLKLIP 180
           AGRRICPGMNMGI+ +ELALAN+LL F+W+LPNG+KEEDVDMEED GL+ +KK PL+LIP
Sbjct: 444 AGRRICPGMNMGIIIVELALANMLLCFDWKLPNGMKEEDVDMEEDAGLSASKKLPLQLIP 503

Query: 181 IAY 184
           I Y
Sbjct: 504 IRY 506

BLAST of Lsi04G006130 vs. NCBI nr
Match: gi|449435434|ref|XP_004135500.1| (PREDICTED: cytochrome P450 71B37-like [Cucumis sativus])

HSP 1 Score: 298.5 bits (763), Expect = 7.9e-78
Identity = 140/184 (76.09%), Postives = 165/184 (89.67%), Query Frame = 1

Query: 1   MAELVKNPRVMKKLQDEIRSCIKED-LVKENDLEKLQYLKVVVKEVLRLHPPAPLLLPRE 60
           MAEL+KNP+VMKKLQ+EIR+C+KE+ +VKENDL+ L+YLK VVKEVLRLH PAPLLLPRE
Sbjct: 305 MAELIKNPKVMKKLQNEIRNCVKENKMVKENDLQNLEYLKAVVKEVLRLHTPAPLLLPRE 364

Query: 61  TMSQFKLDSYDIDPKTHLHVNVWAIGRDPNYWTHPEEFLPERFMGSNIDYKGQNFELLPF 120
            MS FKL+ YDI PKTH++VN WAIGRDP  WT+PEEF+PERF+GSNIDYKGQNFELLPF
Sbjct: 365 AMSHFKLNGYDILPKTHIYVNAWAIGRDPEIWTNPEEFIPERFIGSNIDYKGQNFELLPF 424

Query: 121 GAGRRICPGMNMGIVTMELALANLLLQFNWQLPNGIKEEDVDMEEDVGLTVAKKSPLKLI 180
           G+GRRICPGMNM   T+ELALAN+LL F+W+L NG+KEEDVDMEE+ GL VAKKSPL+L+
Sbjct: 425 GSGRRICPGMNMASFTVELALANVLLCFDWKLANGMKEEDVDMEEETGLAVAKKSPLQLV 484

Query: 181 PIAY 184
           P+ Y
Sbjct: 485 PVHY 488

BLAST of Lsi04G006130 vs. NCBI nr
Match: gi|659090661|ref|XP_008446134.1| (PREDICTED: cytochrome P450 71B2-like [Cucumis melo])

HSP 1 Score: 297.4 bits (760), Expect = 1.8e-77
Identity = 137/184 (74.46%), Postives = 164/184 (89.13%), Query Frame = 1

Query: 1   MAELVKNPRVMKKLQDEIRSCIKED-LVKENDLEKLQYLKVVVKEVLRLHPPAPLLLPRE 60
           M EL+KNP+VMKKLQ+EIR+C+KE  +VKE+DL+ L+YLK VVKEVLRLH PAPLLLPRE
Sbjct: 229 MTELIKNPKVMKKLQNEIRNCVKESTIVKESDLQNLEYLKAVVKEVLRLHTPAPLLLPRE 288

Query: 61  TMSQFKLDSYDIDPKTHLHVNVWAIGRDPNYWTHPEEFLPERFMGSNIDYKGQNFELLPF 120
            MS FKL+ YDI PKTH+HVNVWAIGRD   WT+PEEF+PERF+GSNIDYKGQNFE LPF
Sbjct: 289 AMSHFKLNGYDILPKTHIHVNVWAIGRDSEIWTNPEEFIPERFIGSNIDYKGQNFEFLPF 348

Query: 121 GAGRRICPGMNMGIVTMELALANLLLQFNWQLPNGIKEEDVDMEEDVGLTVAKKSPLKLI 180
           G+GRRICPGMNM   T+ELALAN+LL F+W+LPNG+KEED+DMEE++GLT +KKSPL+L+
Sbjct: 349 GSGRRICPGMNMASFTVELALANMLLCFDWKLPNGVKEEDIDMEEELGLTASKKSPLQLV 408

Query: 181 PIAY 184
           P+ Y
Sbjct: 409 PVHY 412

BLAST of Lsi04G006130 vs. NCBI nr
Match: gi|778708641|ref|XP_011656251.1| (PREDICTED: cytochrome P450 71B10-like [Cucumis sativus])

HSP 1 Score: 296.6 bits (758), Expect = 3.0e-77
Identity = 143/183 (78.14%), Postives = 159/183 (86.89%), Query Frame = 1

Query: 1   MAELVKNPRVMKKLQDEIRSCIKEDLVKENDLEKLQYLKVVVKEVLRLHPPAPLLLPRET 60
           M EL++NPRVMKKLQD IRS IK+D VKE DLE+L YLK+VVKEVLRLHP APLLLPRET
Sbjct: 287 MTELIRNPRVMKKLQDHIRSHIKKDQVKEMDLERLPYLKMVVKEVLRLHPAAPLLLPRET 346

Query: 61  MSQFKLDSYDIDPKTHLHVNVWAIGRDPNYWTHPEEFLPERFMGSNIDYKGQNFELLPFG 120
            S FKL+ YDI PKTHLHVNVWAIGRDP  WT+PEEF PERF  SNIDYKGQN+ELLPFG
Sbjct: 347 TSHFKLNGYDIHPKTHLHVNVWAIGRDPECWTNPEEFFPERFTESNIDYKGQNYELLPFG 406

Query: 121 AGRRICPGMNMGIVTMELALANLLLQFNWQLPNGIKEEDVDMEEDVGLTVAKKSPLKLIP 180
            GRR+C GMNMGI T+EL LANLLL F+W+L +G+KEEDVDMEED GLTVAKKSPL+L+P
Sbjct: 407 GGRRVCAGMNMGIFTIELTLANLLLCFDWKLGDGMKEEDVDMEEDFGLTVAKKSPLELVP 466

Query: 181 IAY 184
           I Y
Sbjct: 467 IPY 469

BLAST of Lsi04G006130 vs. NCBI nr
Match: gi|778704855|ref|XP_004135281.2| (PREDICTED: cytochrome P450 71B2-like isoform X1 [Cucumis sativus])

HSP 1 Score: 291.2 bits (744), Expect = 1.3e-75
Identity = 138/184 (75.00%), Postives = 161/184 (87.50%), Query Frame = 1

Query: 1   MAELVKNPRVMKKLQDEIRSCIKEDLVKENDLEKLQYLKVVVKEVLRLHPPAPLLLPRET 60
           MAEL++ PRVMKKLQD+IRS IK++ VKE DLE+L YLK+VVKEVLRLHPP PLL+PRET
Sbjct: 327 MAELIRKPRVMKKLQDQIRSYIKKEQVKETDLERLPYLKMVVKEVLRLHPPVPLLIPRET 386

Query: 61  MSQFKLDSYDIDPKTHLHVNVWAIGRDPNYWTHPEEFLPERFMGSNIDYKGQNFELLPFG 120
            S FKL+ YDI PK HLHVNVWAIGRDP  W +PEEF+PERF+ +NIDYKGQN+ELLPFG
Sbjct: 387 TSHFKLNGYDIHPKAHLHVNVWAIGRDPECWVNPEEFIPERFIENNIDYKGQNYELLPFG 446

Query: 121 AGRRICPGMNMGIVTMELALANLLLQFNWQLPNGIKEEDVDMEED-VGLTVAKKSPLKLI 180
            GRR+CPGMNMGI T+EL LANLLL F+W+L +G+KEEDVDMEED  G++VAKKSPLKL+
Sbjct: 447 GGRRVCPGMNMGIFTVELTLANLLLCFDWKLGDGMKEEDVDMEEDFFGISVAKKSPLKLV 506

Query: 181 PIAY 184
           PI Y
Sbjct: 507 PIPY 510

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C71BO_ARATH1.6e-6262.37Cytochrome P450 71B24 OS=Arabidopsis thaliana GN=CYP71B24 PE=2 SV=1[more]
C71BQ_ARATH6.1e-6261.96Cytochrome P450 71B26 OS=Arabidopsis thaliana GN=CYP71B26 PE=2 SV=1[more]
C71B9_ARATH7.9e-6260.33Cytochrome P450 71B9 OS=Arabidopsis thaliana GN=CYP71B9 PE=2 SV=3[more]
C71B4_ARATH2.6e-6063.04Cytochrome P450 71B4 OS=Arabidopsis thaliana GN=CYP71B4 PE=2 SV=2[more]
C71BV_ARATH3.3e-6060.87Cytochrome P450 71B34 OS=Arabidopsis thaliana GN=CYP71B34 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
W9RKJ2_9ROSA5.2e-6867.93Cytochrome P450 OS=Morus notabilis GN=L484_027660 PE=3 SV=1[more]
F6GVN1_VITVI1.7e-6667.39Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0137g00540 PE=3 SV=... [more]
W9S1E6_9ROSA2.8e-6666.85Cytochrome P450 OS=Morus notabilis GN=L484_027664 PE=3 SV=1[more]
F6GVM9_VITVI4.8e-6666.30Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0137g00560 PE=3 SV=... [more]
A5AY94_VITVI9.1e-6565.22Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_023587 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G26230.19.0e-6462.37 cytochrome P450, family 71, subfamily B, polypeptide 24[more]
AT3G26290.13.4e-6361.96 cytochrome P450, family 71, subfamily B, polypeptide 26[more]
AT2G02580.14.5e-6360.33 cytochrome P450, family 71, subfamily B, polypeptide 9[more]
AT3G26280.11.4e-6163.04 cytochrome P450, family 71, subfamily B, polypeptide 4[more]
AT3G26300.11.9e-6160.87 cytochrome P450, family 71, subfamily B, polypeptide 34[more]
Match NameE-valueIdentityDescription
gi|659092302|ref|XP_008447001.1|1.3e-8078.69PREDICTED: cytochrome P450 71B34-like [Cucumis melo][more]
gi|449435434|ref|XP_004135500.1|7.9e-7876.09PREDICTED: cytochrome P450 71B37-like [Cucumis sativus][more]
gi|659090661|ref|XP_008446134.1|1.8e-7774.46PREDICTED: cytochrome P450 71B2-like [Cucumis melo][more]
gi|778708641|ref|XP_011656251.1|3.0e-7778.14PREDICTED: cytochrome P450 71B10-like [Cucumis sativus][more]
gi|778704855|ref|XP_004135281.2|1.3e-7575.00PREDICTED: cytochrome P450 71B2-like isoform X1 [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0020037heme binding
GO:0005506iron ion binding
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Vocabulary: INTERPRO
TermDefinition
IPR017972Cyt_P450_CS
IPR002401Cyt_P450_E_grp-I
IPR001128Cyt_P450
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098542 defense response to other organism
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0044550 secondary metabolite biosynthetic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005575 cellular_component
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G006130.1Lsi04G006130.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 126..137
score: 1.6E-10coord: 6..19
score: 1.6E-10coord: 40..51
score: 1.6E-10coord: 117..126
score: 1.6
IPR001128Cytochrome P450GENE3DG3DSA:1.10.630.10coord: 1..181
score: 1.1
IPR001128Cytochrome P450PFAMPF00067p450coord: 2..169
score: 5.4
IPR001128Cytochrome P450unknownSSF48264Cytochrome P450coord: 1..180
score: 7.33
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 126..149
score: 2.3E-21coord: 39..57
score: 2.3E-21coord: 116..126
score: 2.3E-21coord: 80..104
score: 2.3
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 119..128
scor
NoneNo IPR availablePANTHERPTHR24298FAMILY NOT NAMEDcoord: 1..183
score: 2.0E
NoneNo IPR availablePANTHERPTHR24298:SF144BIFUNCTIONAL DIHYDROCAMALEXATE SYNTHASE/CAMALEXIN SYNTHASE-RELATEDcoord: 1..183
score: 2.0E

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Lsi04G006130Cucsa.286660Cucumber (Gy14) v1cgylsiB465
Lsi04G006130MELO3C011934.2Melon (DHL92) v3.6.1lsimedB297
Lsi04G006130Cla97C05G108160Watermelon (97103) v2lsiwmbB284
Lsi04G006130Bhi07G001458Wax gourdlsiwgoB380
The following gene(s) are paralogous to this gene:

None