BLAST of Lsi04G005680 vs. Swiss-Prot
Match:
NRT25_ARATH (High affinity nitrate transporter 2.5 OS=Arabidopsis thaliana GN=NRT2.5 PE=2 SV=1)
HSP 1 Score: 550.4 bits (1417), Expect = 1.8e-155
Identity = 276/392 (70.41%), Postives = 327/392 (83.42%), Query Frame = 1
Query: 1 MEVDG-----GATAAAAGQPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACF 60
MEV+G G T A PR+F+LPVD+E+K+T F +FSVA PHMR FHLSW F CF
Sbjct: 1 MEVEGKGGEAGTTTTTA--PRRFALPVDAENKATTFRLFSVAKPHMRAFHLSWFQFFCCF 60
Query: 61 ISSFAAAPLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLIL 120
+S+FAA PLLPVIR+NLNLT TDIGNAGIASVSGAVFARI+MG+ACDL GPRLASA+L L
Sbjct: 61 VSTFAAPPLLPVIRENLNLTATDIGNAGIASVSGAVFARIVMGTACDLFGPRLASAALTL 120
Query: 121 ITSPAVYLTSIASSPVSFLLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGN 180
T+PAVY T+ SP+ F++VRFF GFSLATFVSTQFWMSSMFS VVG+ANG+A GWGN
Sbjct: 121 STAPAVYFTAGIKSPIGFIMVRFFAGFSLATFVSTQFWMSSMFSGPVVGSANGIAAGWGN 180
Query: 181 LGGGATQLIMPYVFAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRR 240
LGGGATQLIMP VF++I ++GATKFTAWRIAFFIP LFQTLSAFAVLL G+D PDG++
Sbjct: 181 LGGGATQLIMPIVFSLIRNMGATKFTAWRIAFFIPGLFQTLSAFAVLLFGQDLPDGDYWA 240
Query: 241 LQKTGEKAKDKLSKVVYNGVRNYRGWILALTYGYSFGVELTIDNVIANYFYDRFNLNLHT 300
+ K+GE+ KD + KV+ NG++NYRGWI AL YGY FGVELTIDN+IA YF+DRF+L L T
Sbjct: 241 MHKSGEREKDDVGKVISNGIKNYRGWITALAYGYCFGVELTIDNIIAEYFFDRFHLKLQT 300
Query: 301 AGIVAASFGLANLFSRPYGGVLSDVVGKRYGMRGRLWALWLVQTIGGILCVILGLVNSLS 360
AGI+AASFGLAN F+RP GG+ SD + +R+GMRGRLWA W+VQT GG+LC LG ++SL+
Sbjct: 301 AGIIAASFGLANFFARPGGGIFSDFMSRRFGMRGRLWAWWIVQTSGGVLCACLGQISSLT 360
Query: 361 ASIAIMIVFSVFVQAACGLTFGVVPFVSRSML 388
SI +M+VFSVFVQAACGLTFGVVPF+SR L
Sbjct: 361 VSIIVMLVFSVFVQAACGLTFGVVPFISRRSL 390
BLAST of Lsi04G005680 vs. Swiss-Prot
Match:
NRT23_ORYSJ (High-affinity nitrate transporter 2.3 OS=Oryza sativa subsp. japonica GN=NRT2.3 PE=1 SV=1)
HSP 1 Score: 521.5 bits (1342), Expect = 8.8e-147
Identity = 267/388 (68.81%), Postives = 317/388 (81.70%), Query Frame = 1
Query: 1 MEVDGGATAAAAGQPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFA 60
MEV+G AA G+PR F +PVDS+ K+T+F +FS A PHM +FH++W SF CF+S+FA
Sbjct: 8 MEVEG--VEAAGGKPR-FRMPVDSDLKATEFWLFSFARPHMASFHMAWFSFFCCFVSTFA 67
Query: 61 AAPLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPA 120
A PLLP+IRD L LT TDIGNAGIASVSGAVFAR+ MG+ACDLVGPRLASASLIL+T+PA
Sbjct: 68 APPLLPLIRDTLGLTATDIGNAGIASVSGAVFARLAMGTACDLVGPRLASASLILLTTPA 127
Query: 121 VYLTSIASSPVSFLLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGA 180
VY +SI SP +LLVRFFTG SLA+FVS QFWMSSMFS+ VG ANGVAGGWGNLGGGA
Sbjct: 128 VYCSSIIQSPSGYLLVRFFTGISLASFVSAQFWMSSMFSAPKVGLANGVAGGWGNLGGGA 187
Query: 181 TQLIMPYVFAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTG 240
QL+MP V+ I IG+T FTAWRIAFFIP L QT SA AVL G+D P GN+ +L KTG
Sbjct: 188 VQLLMPLVYEAIHKIGSTPFTAWRIAFFIPGLMQTFSAIAVLAFGQDMPGGNYGKLHKTG 247
Query: 241 EKAKDKLSKVVYNGVRNYRGWILALTYGYSFGVELTIDNVIANYFYDRFNLNLHTAGIVA 300
+ KD V+ + + NYRGWILALTYGYSFGVELTIDNV+ YFYDRF++NL TAG++A
Sbjct: 248 DMHKDSFGNVLRHALTNYRGWILALTYGYSFGVELTIDNVVHQYFYDRFDVNLQTAGLIA 307
Query: 301 ASFGLANLFSRPYGGVLSDVVGKRYGMRGRLWALWLVQTIGGILCVILGLVN-SLSASIA 360
ASFG+AN+ SRP GG+LSD + RYGMRGRLW LW VQTIGG+LCV+LG+V+ S +AS+A
Sbjct: 308 ASFGMANIISRPGGGLLSDWLSSRYGMRGRLWGLWTVQTIGGVLCVVLGIVDFSFAASVA 367
Query: 361 IMIVFSVFVQAACGLTFGVVPFVSRSML 388
+M++FS FVQAACGLTFG+VPFVSR L
Sbjct: 368 VMVLFSFFVQAACGLTFGIVPFVSRRSL 392
BLAST of Lsi04G005680 vs. Swiss-Prot
Match:
NRT21_ARATH (High-affinity nitrate transporter 2.1 OS=Arabidopsis thaliana GN=NRT2.1 PE=1 SV=1)
HSP 1 Score: 459.1 bits (1180), Expect = 5.4e-128
Identity = 220/371 (59.30%), Postives = 277/371 (74.66%), Query Frame = 1
Query: 17 KFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPLLPVIRDNLNLTD 76
KF LPVD+EHK+T F +FS A PHMRTFHLSWISF CF+S+FAAAPL+P+IR+NLNLT
Sbjct: 38 KFDLPVDTEHKATVFKLFSFAKPHMRTFHLSWISFSTCFVSTFAAAPLVPIIRENLNLTK 97
Query: 77 TDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPAVYLTSIASSPVSFLLV 136
DIGNAG+ASVSG++F+R++MG+ CDL+GPR A L+++++P V+ S S F+ V
Sbjct: 98 QDIGNAGVASVSGSIFSRLVMGAVCDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITV 157
Query: 137 RFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGATQLIMPYVFAIIEHIG 196
RF GF LATFVS Q+WMS+MF+S ++G NG A GWGN+GGG TQL+MP V+ II G
Sbjct: 158 RFMIGFCLATFVSCQYWMSTMFNSQIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCG 217
Query: 197 ATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTGEKAKDKLSKVVYNGVR 256
+T FTAWRIAFF+P + VL LG+D PDGN L+K GE AKDK K+++ V
Sbjct: 218 STAFTAWRIAFFVPGWLHIIMGILVLNLGQDLPDGNRATLEKAGEVAKDKFGKILWYAVT 277
Query: 257 NYRGWILALTYGYSFGVELTIDNVIANYFYDRFNLNLHTAGIVAASFGLANLFSRPYGGV 316
NYR WI L YGYS GVEL+ DNVIA YF+DRF+L LHTAG++AA FG+AN F+RP GG
Sbjct: 278 NYRTWIFVLLYGYSMGVELSTDNVIAEYFFDRFHLKLHTAGLIAACFGMANFFARPAGGY 337
Query: 317 LSDVVGKRYGMRGRLWALWLVQTIGGILCVILGLVNSLSASIAIMIVFSVFVQAACGLTF 376
SD K +GMRGRLW LW++QT GG+ CV LG N+L ++ M++FS+ QAACG TF
Sbjct: 338 ASDFAAKYFGMRGRLWTLWIIQTAGGLFCVWLGRANTLVTAVVAMVLFSMGAQAACGATF 397
Query: 377 GVVPFVSRSML 388
+VPFVSR L
Sbjct: 398 AIVPFVSRRAL 408
BLAST of Lsi04G005680 vs. Swiss-Prot
Match:
NRT22_ARATH (High-affinity nitrate transporter 2.2 OS=Arabidopsis thaliana GN=NRT2.2 PE=2 SV=1)
HSP 1 Score: 456.8 bits (1174), Expect = 2.7e-127
Identity = 218/372 (58.60%), Postives = 279/372 (75.00%), Query Frame = 1
Query: 16 RKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPLLPVIRDNLNLT 75
+K++LPVD+E K+T F +FS A PHMRTFHLSWISF CF+S+FAAAPL+P+IR+NLNLT
Sbjct: 35 KKYNLPVDAEDKATVFKLFSFAKPHMRTFHLSWISFSTCFVSTFAAAPLIPIIRENLNLT 94
Query: 76 DTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPAVYLTSIASSPVSFLL 135
DIGNAG+ASVSG++F+R++MG+ CDL+GPR A L+++++P V+ S S F+
Sbjct: 95 KHDIGNAGVASVSGSIFSRLVMGAVCDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFIT 154
Query: 136 VRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGATQLIMPYVFAIIEHI 195
VRF GF LATFVS Q+WMS+MF+S ++G NG A GWGN+GGG TQL+MP V+ II
Sbjct: 155 VRFMIGFCLATFVSCQYWMSTMFNSQIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRC 214
Query: 196 GATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTGEKAKDKLSKVVYNGV 255
G+T FTAWRIAFF+P + VL LG+D P GN ++K GE AKDK K+++ V
Sbjct: 215 GSTAFTAWRIAFFVPGWLHIIMGILVLTLGQDLPGGNRAAMEKAGEVAKDKFGKILWYAV 274
Query: 256 RNYRGWILALTYGYSFGVELTIDNVIANYFYDRFNLNLHTAGIVAASFGLANLFSRPYGG 315
NYR WI L YGYS GVEL+ DNVIA YF+DRF+L LHTAGI+AA FG+AN F+RP GG
Sbjct: 275 TNYRTWIFVLLYGYSMGVELSTDNVIAEYFFDRFHLKLHTAGIIAACFGMANFFARPAGG 334
Query: 316 VLSDVVGKRYGMRGRLWALWLVQTIGGILCVILGLVNSLSASIAIMIVFSVFVQAACGLT 375
SD+ KR+GMRGRLW LW++QT GG+ CV LG N+L ++ M++FS+ QAACG T
Sbjct: 335 WASDIAAKRFGMRGRLWTLWIIQTSGGLFCVWLGRANTLVTAVVSMVLFSLGAQAACGAT 394
Query: 376 FGVVPFVSRSML 388
F +VPFVSR L
Sbjct: 395 FAIVPFVSRRAL 406
BLAST of Lsi04G005680 vs. Swiss-Prot
Match:
NRT21_ORYSJ (High-affinity nitrate transporter 2.1 OS=Oryza sativa subsp. japonica GN=NRT2.1 PE=1 SV=1)
HSP 1 Score: 453.4 bits (1165), Expect = 2.9e-126
Identity = 222/383 (57.96%), Postives = 284/383 (74.15%), Query Frame = 1
Query: 5 GGATAAAAGQPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPL 64
GG AAAA KF LPVDSEHK+ + S A PHMRTFHLSWISF +CF+S+FAAAPL
Sbjct: 30 GGEDAAAAS---KFDLPVDSEHKAKTIRLLSFANPHMRTFHLSWISFFSCFVSTFAAAPL 89
Query: 65 LPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPAVYLT 124
+P+IRDNLNLT DIGNAG+ASVSG++F+R+ MG+ CD++GPR A LI++ +P V+
Sbjct: 90 VPIIRDNLNLTKADIGNAGVASVSGSIFSRLAMGAICDMLGPRYGCAFLIMLAAPTVFCM 149
Query: 125 SIASSPVSFLLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGATQLI 184
S+ S ++ VRF GFSLATFVS Q+WMS+MF+S ++G NG+A GWGN+GGGATQLI
Sbjct: 150 SLIDSAAGYIAVRFLIGFSLATFVSCQYWMSTMFNSKIIGLVNGLAAGWGNMGGGATQLI 209
Query: 185 MPYVFAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTGEKAK 244
MP V+ +I GAT FTAWR+A+F+P + VL LG+D PDGN R LQK G+ +
Sbjct: 210 MPLVYDVIRKCGATPFTAWRLAYFVPGTLHVVMGVLVLTLGQDLPDGNLRSLQKKGDVNR 269
Query: 245 DKLSKVVYNGVRNYRGWILALTYGYSFGVELTIDNVIANYFYDRFNLNLHTAGIVAASFG 304
D S+V++ V NYR WI L YGYS GVELT DNVIA YFYDRF+L+L AGI+AASFG
Sbjct: 270 DSFSRVLWYAVTNYRTWIFVLLYGYSMGVELTTDNVIAEYFYDRFDLDLRVAGIIAASFG 329
Query: 305 LANLFSRPYGGVLSDVVGKRYGMRGRLWALWLVQTIGGILCVILGLVNSLSASIAIMIVF 364
+AN+ +RP GG+LSD+ + +GMR RLW +W++QT GG C++LG ++L S+ M++F
Sbjct: 330 MANIVARPTGGLLSDLGARYFGMRARLWNIWILQTAGGAFCLLLGRASTLPTSVVCMVLF 389
Query: 365 SVFVQAACGLTFGVVPFVSRSML 388
S QAACG FGV+PFVSR L
Sbjct: 390 SFCAQAACGAIFGVIPFVSRRSL 409
BLAST of Lsi04G005680 vs. TrEMBL
Match:
S4VIV8_CUCSA (High-affinity nitrate transporter NRT2.3 OS=Cucumis sativus GN=NRT2.3 PE=2 SV=1)
HSP 1 Score: 686.0 bits (1769), Expect = 3.0e-194
Identity = 353/376 (93.88%), Postives = 363/376 (96.54%), Query Frame = 1
Query: 12 AGQPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPLLPVIRDN 71
A RKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPLLPVIRDN
Sbjct: 4 APHQRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPLLPVIRDN 63
Query: 72 LNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPAVYLTSIASSPV 131
LNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPR+ASASLILITSPAVYLTSI SSPV
Sbjct: 64 LNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRIASASLILITSPAVYLTSIVSSPV 123
Query: 132 SFLLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGATQLIMPYVFAI 191
+F L RFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGATQLIMPYVFAI
Sbjct: 124 TFFLARFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGATQLIMPYVFAI 183
Query: 192 IEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTGEKAKDKLSKVV 251
IEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNF RL+KTGEKAKDK SKVV
Sbjct: 184 IEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFGRLKKTGEKAKDKFSKVV 243
Query: 252 YNGVRNYRGWILALTYGYSFGVELTIDNVIANYFYDRFNLNLHTAGIVAASFGLANLFSR 311
YNGV NYRGWILALTYGY FGVELTIDNVIANYFYDRFNLNLHTAGIVAASFGLANLFSR
Sbjct: 244 YNGVTNYRGWILALTYGYCFGVELTIDNVIANYFYDRFNLNLHTAGIVAASFGLANLFSR 303
Query: 312 PYGGVLSDVVGKRYGMRGRLWALWLVQTIGGILCVILGLVNSLSASIAIMIVFSVFVQAA 371
PYGGVLSDVV KR+GMRGRLWALWLVQTIGGILCV+LGLV+SLS+SIA+M++FSVFVQAA
Sbjct: 304 PYGGVLSDVVAKRFGMRGRLWALWLVQTIGGILCVVLGLVDSLSSSIAVMLIFSVFVQAA 363
Query: 372 CGLTFGVVPFVSRSML 388
CGLTFGVVPFVSR L
Sbjct: 364 CGLTFGVVPFVSRRSL 379
BLAST of Lsi04G005680 vs. TrEMBL
Match:
A0A061E862_THECC (Nitrate transporter2.5 OS=Theobroma cacao GN=TCM_010451 PE=4 SV=1)
HSP 1 Score: 608.2 bits (1567), Expect = 7.9e-171
Identity = 301/374 (80.48%), Postives = 340/374 (90.91%), Query Frame = 1
Query: 14 QPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPLLPVIRDNLN 73
QP+KF+LPVDSEHK+T+F +FSVAAPHMR FHLSWISF ACF+S+FAA PLLP+IRDNLN
Sbjct: 11 QPQKFALPVDSEHKATEFRLFSVAAPHMRAFHLSWISFFACFVSTFAAPPLLPIIRDNLN 70
Query: 74 LTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPAVYLTSIASSPVSF 133
LT TDIGNAG+ASVSGAVFARI MG+ACDL GPRLASASLIL+T+PAVY TSIASSPVSF
Sbjct: 71 LTATDIGNAGVASVSGAVFARIAMGTACDLFGPRLASASLILLTAPAVYFTSIASSPVSF 130
Query: 134 LLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGATQLIMPYVFAIIE 193
LLVRFFTGFSLATFVSTQFWMSSMFS+ VVGTANGVA GWGNLGGGATQLIMP VF++I
Sbjct: 131 LLVRFFTGFSLATFVSTQFWMSSMFSTPVVGTANGVAAGWGNLGGGATQLIMPLVFSVIR 190
Query: 194 HIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTGEKAKDKLSKVVYN 253
IGA KFTAWRIAFFIPALFQTL+AFA+L+ G+D PDGN++RLQK+G K KDK S+V Y+
Sbjct: 191 DIGAVKFTAWRIAFFIPALFQTLAAFAILIFGQDLPDGNYQRLQKSGTKQKDKFSRVFYH 250
Query: 254 GVRNYRGWILALTYGYSFGVELTIDNVIANYFYDRFNLNLHTAGIVAASFGLANLFSRPY 313
G+ NYRGWILALTYGY FGVELT+DN+IA YFYDRFNL LHTAGI+AASFGLANLFSRP
Sbjct: 251 GITNYRGWILALTYGYCFGVELTVDNIIAEYFYDRFNLKLHTAGIIAASFGLANLFSRPA 310
Query: 314 GGVLSDVVGKRYGMRGRLWALWLVQTIGGILCVILGLVNSLSASIAIMIVFSVFVQAACG 373
GG++SD + R+GMRGRLWALW++QT+GG+ C+ILG V SLSASI +MI+FSVFVQAACG
Sbjct: 311 GGIISDRMASRFGMRGRLWALWIIQTLGGVFCIILGQVGSLSASIIVMIIFSVFVQAACG 370
Query: 374 LTFGVVPFVSRSML 388
LTFGVVPFVSR L
Sbjct: 371 LTFGVVPFVSRRSL 384
BLAST of Lsi04G005680 vs. TrEMBL
Match:
B9IEW2_POPTR (High-affinity nitrate transporter family protein OS=Populus trichocarpa GN=POPTR_0015s09290g PE=4 SV=2)
HSP 1 Score: 602.4 bits (1552), Expect = 4.4e-169
Identity = 303/387 (78.29%), Postives = 342/387 (88.37%), Query Frame = 1
Query: 1 MEVDGGATAAAAGQPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFA 60
ME++G AT + QP KF+LPVDSEHK+T+F +FSVAAPHMR FHLSW+SF ACF+S+FA
Sbjct: 1 MEIEGQATVKES-QPPKFALPVDSEHKATEFRLFSVAAPHMRAFHLSWVSFFACFVSTFA 60
Query: 61 AAPLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPA 120
A PLLP+IRDNLNLT +DIGNAGIASVSGAVFAR+ MG+ACDL GPRLASASLILIT+PA
Sbjct: 61 APPLLPIIRDNLNLTASDIGNAGIASVSGAVFARVAMGTACDLFGPRLASASLILITAPA 120
Query: 121 VYLTSIASSPVSFLLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGA 180
VY TSIASS SFLLVRFFTGFSLATFVSTQFWMSSMFS+ VVGTANGVAGGWGNLGGGA
Sbjct: 121 VYFTSIASSSTSFLLVRFFTGFSLATFVSTQFWMSSMFSAPVVGTANGVAGGWGNLGGGA 180
Query: 181 TQLIMPYVFAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTG 240
TQLIMP VF +I IGA KFTAWRIAFFIPALFQTLSAFAVL+ G+D PDGNF RLQK G
Sbjct: 181 TQLIMPLVFGLIRDIGAIKFTAWRIAFFIPALFQTLSAFAVLIFGKDLPDGNFGRLQKAG 240
Query: 241 EKAKDKLSKVVYNGVRNYRGWILALTYGYSFGVELTIDNVIANYFYDRFNLNLHTAGIVA 300
+K KDK S V Y+G++NYRGWILAL+YGY FGVELTIDN++A YFYDRF+L LHTAG++A
Sbjct: 241 DKTKDKFSNVFYHGIKNYRGWILALSYGYCFGVELTIDNIVAEYFYDRFDLKLHTAGMIA 300
Query: 301 ASFGLANLFSRPYGGVLSDVVGKRYGMRGRLWALWLVQTIGGILCVILGLVNSLSASIAI 360
ASFGLAN+ SRP GG++SD VGKR+GMRGRLWALW+ QT+GG+ C+ILG V SL ASI +
Sbjct: 301 ASFGLANIVSRPGGGMISDAVGKRFGMRGRLWALWIAQTLGGVFCIILGRVGSLGASIVV 360
Query: 361 MIVFSVFVQAACGLTFGVVPFVSRSML 388
MIVFS+F QAACGLTFGVVPFVSR L
Sbjct: 361 MIVFSLFCQAACGLTFGVVPFVSRRSL 386
BLAST of Lsi04G005680 vs. TrEMBL
Match:
Q5K409_9ROSI (Nitrate transporter OS=Populus tremula x Populus tremuloides GN=tNit2.2 PE=2 SV=1)
HSP 1 Score: 602.1 bits (1551), Expect = 5.7e-169
Identity = 302/387 (78.04%), Postives = 343/387 (88.63%), Query Frame = 1
Query: 1 MEVDGGATAAAAGQPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFA 60
ME++G AT + QP KF+LPVDSEHK+T+F +FSVAAPHMR FHLSW+SF ACF+SSFA
Sbjct: 1 MEIEGQATVKES-QPPKFALPVDSEHKATEFRLFSVAAPHMRAFHLSWVSFFACFVSSFA 60
Query: 61 AAPLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPA 120
A PLLP+IRDNLNLT +DIGNAGIASVSGAVFAR+ MG+ACDL GPRLASASLIL+T+PA
Sbjct: 61 APPLLPIIRDNLNLTASDIGNAGIASVSGAVFARVAMGTACDLFGPRLASASLILLTAPA 120
Query: 121 VYLTSIASSPVSFLLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGA 180
VY TS+ASS SFLLVRFFTGFSLATFVSTQFWMSSMFS+ VVGTANGVAGGWGNLGGGA
Sbjct: 121 VYFTSMASSSTSFLLVRFFTGFSLATFVSTQFWMSSMFSAPVVGTANGVAGGWGNLGGGA 180
Query: 181 TQLIMPYVFAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTG 240
TQLIMP VF +I IGA KFTAWRIAFFIPALFQTLSAFAVL+ G+D PDGNFRRLQK G
Sbjct: 181 TQLIMPLVFGLIRDIGAIKFTAWRIAFFIPALFQTLSAFAVLIFGKDLPDGNFRRLQKAG 240
Query: 241 EKAKDKLSKVVYNGVRNYRGWILALTYGYSFGVELTIDNVIANYFYDRFNLNLHTAGIVA 300
+K KDK S V Y+G++NYRGWILAL+YGY FGVELTIDN++A YFYDRF+L LHTAG++A
Sbjct: 241 DKTKDKFSNVFYHGIKNYRGWILALSYGYCFGVELTIDNIVAEYFYDRFDLKLHTAGMIA 300
Query: 301 ASFGLANLFSRPYGGVLSDVVGKRYGMRGRLWALWLVQTIGGILCVILGLVNSLSASIAI 360
ASFGLAN+ SRP GG++SD VGKR+GMRG+LWALW+VQT+GG+ C+ILG V SL ASI +
Sbjct: 301 ASFGLANIVSRPGGGMISDAVGKRFGMRGKLWALWVVQTLGGVFCIILGRVGSLGASIVV 360
Query: 361 MIVFSVFVQAACGLTFGVVPFVSRSML 388
MIVF +F QAACGLTFGVVPFVSR L
Sbjct: 361 MIVFFLFCQAACGLTFGVVPFVSRRSL 386
BLAST of Lsi04G005680 vs. TrEMBL
Match:
V7C8S4_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_003G062800g PE=4 SV=1)
HSP 1 Score: 601.7 bits (1550), Expect = 7.4e-169
Identity = 296/387 (76.49%), Postives = 345/387 (89.15%), Query Frame = 1
Query: 1 MEVDGGATAAAAGQPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFA 60
MEV+ A A + +KF+LPVDSEHK+T+F +FS+A PHMR+FHLSW+SF +CF+SSFA
Sbjct: 1 MEVELPAAAENESEQKKFALPVDSEHKATEFRLFSIAKPHMRSFHLSWVSFFSCFVSSFA 60
Query: 61 AAPLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPA 120
A PLLP+IRDNLNLT TDIGNAG+ASVSGAVFAR+ MG+ACDLVGPRLASASLIL+T+P
Sbjct: 61 APPLLPIIRDNLNLTATDIGNAGVASVSGAVFARVAMGTACDLVGPRLASASLILLTAPF 120
Query: 121 VYLTSIASSPVSFLLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGA 180
VY TSI +SP S+LLVRFFTGFSLATFVSTQFWMSSMFS+ VVG+ANG AGGWGNLGGGA
Sbjct: 121 VYFTSIINSPTSYLLVRFFTGFSLATFVSTQFWMSSMFSAPVVGSANGFAGGWGNLGGGA 180
Query: 181 TQLIMPYVFAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTG 240
TQLIMP VF++I IGATKFTAWRIAFF+PA+FQ L+AF++L+ G+D PDGNFRRL+K+G
Sbjct: 181 TQLIMPLVFSLIRDIGATKFTAWRIAFFVPAMFQMLTAFSILIFGQDMPDGNFRRLKKSG 240
Query: 241 EKAKDKLSKVVYNGVRNYRGWILALTYGYSFGVELTIDNVIANYFYDRFNLNLHTAGIVA 300
+KAKD S+VVY+G+ NYRGWILALTYGY FGVELTIDN+IA YFYDRFNL LHTAGI+A
Sbjct: 241 DKAKDDFSRVVYHGITNYRGWILALTYGYCFGVELTIDNIIAEYFYDRFNLTLHTAGIIA 300
Query: 301 ASFGLANLFSRPYGGVLSDVVGKRYGMRGRLWALWLVQTIGGILCVILGLVNSLSASIAI 360
ASFGLANLFSRP GG +SDV+GKR+GMRGRLWALWL QT+ G+ CVILGLV SLS S+ +
Sbjct: 301 ASFGLANLFSRPGGGYISDVMGKRFGMRGRLWALWLCQTLAGVFCVILGLVGSLSVSVLV 360
Query: 361 MIVFSVFVQAACGLTFGVVPFVSRSML 388
MI+FSVFVQAACG+TFG+VPFVSR L
Sbjct: 361 MIIFSVFVQAACGMTFGIVPFVSRRSL 387
BLAST of Lsi04G005680 vs. TAIR10
Match:
AT1G12940.1 (AT1G12940.1 nitrate transporter2.5)
HSP 1 Score: 550.4 bits (1417), Expect = 1.0e-156
Identity = 276/392 (70.41%), Postives = 327/392 (83.42%), Query Frame = 1
Query: 1 MEVDG-----GATAAAAGQPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACF 60
MEV+G G T A PR+F+LPVD+E+K+T F +FSVA PHMR FHLSW F CF
Sbjct: 1 MEVEGKGGEAGTTTTTA--PRRFALPVDAENKATTFRLFSVAKPHMRAFHLSWFQFFCCF 60
Query: 61 ISSFAAAPLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLIL 120
+S+FAA PLLPVIR+NLNLT TDIGNAGIASVSGAVFARI+MG+ACDL GPRLASA+L L
Sbjct: 61 VSTFAAPPLLPVIRENLNLTATDIGNAGIASVSGAVFARIVMGTACDLFGPRLASAALTL 120
Query: 121 ITSPAVYLTSIASSPVSFLLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGN 180
T+PAVY T+ SP+ F++VRFF GFSLATFVSTQFWMSSMFS VVG+ANG+A GWGN
Sbjct: 121 STAPAVYFTAGIKSPIGFIMVRFFAGFSLATFVSTQFWMSSMFSGPVVGSANGIAAGWGN 180
Query: 181 LGGGATQLIMPYVFAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRR 240
LGGGATQLIMP VF++I ++GATKFTAWRIAFFIP LFQTLSAFAVLL G+D PDG++
Sbjct: 181 LGGGATQLIMPIVFSLIRNMGATKFTAWRIAFFIPGLFQTLSAFAVLLFGQDLPDGDYWA 240
Query: 241 LQKTGEKAKDKLSKVVYNGVRNYRGWILALTYGYSFGVELTIDNVIANYFYDRFNLNLHT 300
+ K+GE+ KD + KV+ NG++NYRGWI AL YGY FGVELTIDN+IA YF+DRF+L L T
Sbjct: 241 MHKSGEREKDDVGKVISNGIKNYRGWITALAYGYCFGVELTIDNIIAEYFFDRFHLKLQT 300
Query: 301 AGIVAASFGLANLFSRPYGGVLSDVVGKRYGMRGRLWALWLVQTIGGILCVILGLVNSLS 360
AGI+AASFGLAN F+RP GG+ SD + +R+GMRGRLWA W+VQT GG+LC LG ++SL+
Sbjct: 301 AGIIAASFGLANFFARPGGGIFSDFMSRRFGMRGRLWAWWIVQTSGGVLCACLGQISSLT 360
Query: 361 ASIAIMIVFSVFVQAACGLTFGVVPFVSRSML 388
SI +M+VFSVFVQAACGLTFGVVPF+SR L
Sbjct: 361 VSIIVMLVFSVFVQAACGLTFGVVPFISRRSL 390
BLAST of Lsi04G005680 vs. TAIR10
Match:
AT1G08090.1 (AT1G08090.1 nitrate transporter 2:1)
HSP 1 Score: 459.1 bits (1180), Expect = 3.0e-129
Identity = 220/371 (59.30%), Postives = 277/371 (74.66%), Query Frame = 1
Query: 17 KFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPLLPVIRDNLNLTD 76
KF LPVD+EHK+T F +FS A PHMRTFHLSWISF CF+S+FAAAPL+P+IR+NLNLT
Sbjct: 38 KFDLPVDTEHKATVFKLFSFAKPHMRTFHLSWISFSTCFVSTFAAAPLVPIIRENLNLTK 97
Query: 77 TDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPAVYLTSIASSPVSFLLV 136
DIGNAG+ASVSG++F+R++MG+ CDL+GPR A L+++++P V+ S S F+ V
Sbjct: 98 QDIGNAGVASVSGSIFSRLVMGAVCDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITV 157
Query: 137 RFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGATQLIMPYVFAIIEHIG 196
RF GF LATFVS Q+WMS+MF+S ++G NG A GWGN+GGG TQL+MP V+ II G
Sbjct: 158 RFMIGFCLATFVSCQYWMSTMFNSQIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCG 217
Query: 197 ATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTGEKAKDKLSKVVYNGVR 256
+T FTAWRIAFF+P + VL LG+D PDGN L+K GE AKDK K+++ V
Sbjct: 218 STAFTAWRIAFFVPGWLHIIMGILVLNLGQDLPDGNRATLEKAGEVAKDKFGKILWYAVT 277
Query: 257 NYRGWILALTYGYSFGVELTIDNVIANYFYDRFNLNLHTAGIVAASFGLANLFSRPYGGV 316
NYR WI L YGYS GVEL+ DNVIA YF+DRF+L LHTAG++AA FG+AN F+RP GG
Sbjct: 278 NYRTWIFVLLYGYSMGVELSTDNVIAEYFFDRFHLKLHTAGLIAACFGMANFFARPAGGY 337
Query: 317 LSDVVGKRYGMRGRLWALWLVQTIGGILCVILGLVNSLSASIAIMIVFSVFVQAACGLTF 376
SD K +GMRGRLW LW++QT GG+ CV LG N+L ++ M++FS+ QAACG TF
Sbjct: 338 ASDFAAKYFGMRGRLWTLWIIQTAGGLFCVWLGRANTLVTAVVAMVLFSMGAQAACGATF 397
Query: 377 GVVPFVSRSML 388
+VPFVSR L
Sbjct: 398 AIVPFVSRRAL 408
BLAST of Lsi04G005680 vs. TAIR10
Match:
AT1G08100.1 (AT1G08100.1 nitrate transporter 2.2)
HSP 1 Score: 456.8 bits (1174), Expect = 1.5e-128
Identity = 218/372 (58.60%), Postives = 279/372 (75.00%), Query Frame = 1
Query: 16 RKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPLLPVIRDNLNLT 75
+K++LPVD+E K+T F +FS A PHMRTFHLSWISF CF+S+FAAAPL+P+IR+NLNLT
Sbjct: 35 KKYNLPVDAEDKATVFKLFSFAKPHMRTFHLSWISFSTCFVSTFAAAPLIPIIRENLNLT 94
Query: 76 DTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPAVYLTSIASSPVSFLL 135
DIGNAG+ASVSG++F+R++MG+ CDL+GPR A L+++++P V+ S S F+
Sbjct: 95 KHDIGNAGVASVSGSIFSRLVMGAVCDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFIT 154
Query: 136 VRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGATQLIMPYVFAIIEHI 195
VRF GF LATFVS Q+WMS+MF+S ++G NG A GWGN+GGG TQL+MP V+ II
Sbjct: 155 VRFMIGFCLATFVSCQYWMSTMFNSQIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRC 214
Query: 196 GATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTGEKAKDKLSKVVYNGV 255
G+T FTAWRIAFF+P + VL LG+D P GN ++K GE AKDK K+++ V
Sbjct: 215 GSTAFTAWRIAFFVPGWLHIIMGILVLTLGQDLPGGNRAAMEKAGEVAKDKFGKILWYAV 274
Query: 256 RNYRGWILALTYGYSFGVELTIDNVIANYFYDRFNLNLHTAGIVAASFGLANLFSRPYGG 315
NYR WI L YGYS GVEL+ DNVIA YF+DRF+L LHTAGI+AA FG+AN F+RP GG
Sbjct: 275 TNYRTWIFVLLYGYSMGVELSTDNVIAEYFFDRFHLKLHTAGIIAACFGMANFFARPAGG 334
Query: 316 VLSDVVGKRYGMRGRLWALWLVQTIGGILCVILGLVNSLSASIAIMIVFSVFVQAACGLT 375
SD+ KR+GMRGRLW LW++QT GG+ CV LG N+L ++ M++FS+ QAACG T
Sbjct: 335 WASDIAAKRFGMRGRLWTLWIIQTSGGLFCVWLGRANTLVTAVVSMVLFSLGAQAACGAT 394
Query: 376 FGVVPFVSRSML 388
F +VPFVSR L
Sbjct: 395 FAIVPFVSRRAL 406
BLAST of Lsi04G005680 vs. TAIR10
Match:
AT5G60770.1 (AT5G60770.1 nitrate transporter 2.4)
HSP 1 Score: 453.0 bits (1164), Expect = 2.2e-127
Identity = 214/379 (56.46%), Postives = 278/379 (73.35%), Query Frame = 1
Query: 9 AAAAGQPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPLLPVI 68
A + KFSLPVD+EHK+ F + S APHMRTFHL+WISF CFIS+FAAAPL+P+I
Sbjct: 30 AVPSDSSAKFSLPVDTEHKAKVFKLLSFEAPHMRTFHLAWISFFTCFISTFAAAPLVPII 89
Query: 69 RDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPAVYLTSIAS 128
RDNLNLT D+GNAG+ASVSG++F+R++MG+ CDL+GPR A L+++++P V+ S
Sbjct: 90 RDNLNLTRQDVGNAGVASVSGSIFSRLVMGAVCDLLGPRYGCAFLVMLSAPTVFSMSFVG 149
Query: 129 SPVSFLLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGATQLIMPYV 188
++ VRF GF LATFVS Q+WMS+MF+ ++G NG A GWGN+GGG TQL+MP V
Sbjct: 150 GAGGYITVRFMIGFCLATFVSCQYWMSTMFNGQIIGLVNGTAAGWGNMGGGVTQLLMPMV 209
Query: 189 FAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTGEKAKDKLS 248
+ II +G+T FTAWR+AFF+P + VL LG+D PDGN L+K G KDK S
Sbjct: 210 YEIIRRLGSTSFTAWRMAFFVPGWMHIIMGILVLTLGQDLPDGNRSTLEKKGAVTKDKFS 269
Query: 249 KVVYNGVRNYRGWILALTYGYSFGVELTIDNVIANYFYDRFNLNLHTAGIVAASFGLANL 308
KV++ + NYR W+ L YGYS GVELT DNVIA YF+DRF+L LHTAGI+AASFG+AN
Sbjct: 270 KVLWYAITNYRTWVFVLLYGYSMGVELTTDNVIAEYFFDRFHLKLHTAGIIAASFGMANF 329
Query: 309 FSRPYGGVLSDVVGKRYGMRGRLWALWLVQTIGGILCVILGLVNSLSASIAIMIVFSVFV 368
F+RP GG SD+ +R+GMRGRLW LW++QT+GG C+ LG +L ++ MI+FS+
Sbjct: 330 FARPIGGWASDIAARRFGMRGRLWTLWIIQTLGGFFCLWLGRATTLPTAVVFMILFSLGA 389
Query: 369 QAACGLTFGVVPFVSRSML 388
QAACG TF ++PF+SR L
Sbjct: 390 QAACGATFAIIPFISRRSL 408
BLAST of Lsi04G005680 vs. TAIR10
Match:
AT3G45060.1 (AT3G45060.1 high affinity nitrate transporter 2.6)
HSP 1 Score: 453.0 bits (1164), Expect = 2.2e-127
Identity = 223/371 (60.11%), Postives = 280/371 (75.47%), Query Frame = 1
Query: 17 KFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPLLPVIRDNLNLTD 76
KF+LPVDSEH++ F S A PHMR FHL WISF CFIS+FAAAPL+PVIRDNL+LT
Sbjct: 48 KFALPVDSEHRAKVFKPLSFAKPHMRAFHLGWISFFTCFISTFAAAPLVPVIRDNLDLTK 107
Query: 77 TDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPAVYLTSIASSPVSFLLV 136
TDIGNAG+ASVSGA+F+R+ MG+ CDL+G R +A +++T+PAV+ S + S+L V
Sbjct: 108 TDIGNAGVASVSGAIFSRLAMGAVCDLLGARYGTAFSLMLTAPAVFSMSFVADAGSYLAV 167
Query: 137 RFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGATQLIMPYVFAIIEHIG 196
RF GF LATFVS Q+W S MF+ ++G NG AGGWG++GGG TQL+MP VF +I+ G
Sbjct: 168 RFMIGFCLATFVSCQYWTSVMFTGKIIGLVNGCAGGWGDMGGGVTQLLMPMVFHVIKLTG 227
Query: 197 ATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTGEKAKDKLSKVVYNGVR 256
AT FTAWR AFFIP + Q + VL LG+D PDGN LQK+G+ +KDK SKV + V+
Sbjct: 228 ATPFTAWRFAFFIPGILQIVMGILVLTLGQDLPDGNLSTLQKSGQVSKDKFSKVFWFAVK 287
Query: 257 NYRGWILALTYGYSFGVELTIDNVIANYFYDRFNLNLHTAGIVAASFGLANLFSRPYGGV 316
NYR WIL + YG+S GVELTI+NVI+ YFYDRFNL LHTAGI+AASFG+AN F+RP+GG
Sbjct: 288 NYRTWILFMLYGFSMGVELTINNVISGYFYDRFNLTLHTAGIIAASFGMANFFARPFGGY 347
Query: 317 LSDVVGKRYGMRGRLWALWLVQTIGGILCVILGLVNSLSASIAIMIVFSVFVQAACGLTF 376
SDV + +GMRGRLW LW++QT+G + C+ LG +SL +I M++FS+ QAACG F
Sbjct: 348 ASDVAARLFGMRGRLWILWILQTVGALFCIWLGRASSLPIAILAMMLFSMGTQAACGALF 407
Query: 377 GVVPFVSRSML 388
GV PFVSR L
Sbjct: 408 GVAPFVSRRSL 418
BLAST of Lsi04G005680 vs. NCBI nr
Match:
gi|659090755|ref|XP_008446183.1| (PREDICTED: high affinity nitrate transporter 2.5 [Cucumis melo])
HSP 1 Score: 688.7 bits (1776), Expect = 6.6e-195
Identity = 358/387 (92.51%), Postives = 369/387 (95.35%), Query Frame = 1
Query: 1 MEVDGGATAAAAGQPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFA 60
ME +GGA A RKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFA
Sbjct: 1 MEDNGGAMGAP--HQRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFA 60
Query: 61 AAPLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPA 120
AAPLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPR+ASASLILITSPA
Sbjct: 61 AAPLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRIASASLILITSPA 120
Query: 121 VYLTSIASSPVSFLLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGA 180
VYLTSI SSPV+F L RFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGA
Sbjct: 121 VYLTSIVSSPVTFFLARFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGA 180
Query: 181 TQLIMPYVFAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTG 240
TQLIMPYVFAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNF RL+KTG
Sbjct: 181 TQLIMPYVFAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFGRLKKTG 240
Query: 241 EKAKDKLSKVVYNGVRNYRGWILALTYGYSFGVELTIDNVIANYFYDRFNLNLHTAGIVA 300
EKAKDK SKV+YNGV NYRGWILALTYGY FGVELTIDNVIANYFYDRF+LNLHTAGIVA
Sbjct: 241 EKAKDKFSKVLYNGVTNYRGWILALTYGYCFGVELTIDNVIANYFYDRFDLNLHTAGIVA 300
Query: 301 ASFGLANLFSRPYGGVLSDVVGKRYGMRGRLWALWLVQTIGGILCVILGLVNSLSASIAI 360
ASFGLANLFSRPYGGVLSDVV KRYGMRGRLWALWLVQTIGGILCV+LGLV+SLS+SIAI
Sbjct: 301 ASFGLANLFSRPYGGVLSDVVAKRYGMRGRLWALWLVQTIGGILCVVLGLVDSLSSSIAI 360
Query: 361 MIVFSVFVQAACGLTFGVVPFVSRSML 388
M++FSVFVQAACGLTFGVVPFVSR L
Sbjct: 361 MLIFSVFVQAACGLTFGVVPFVSRRSL 385
BLAST of Lsi04G005680 vs. NCBI nr
Match:
gi|821595461|ref|NP_001295862.1| (high affinity nitrate transporter 2.5 [Cucumis sativus])
HSP 1 Score: 686.0 bits (1769), Expect = 4.3e-194
Identity = 353/376 (93.88%), Postives = 363/376 (96.54%), Query Frame = 1
Query: 12 AGQPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPLLPVIRDN 71
A RKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPLLPVIRDN
Sbjct: 4 APHQRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPLLPVIRDN 63
Query: 72 LNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPAVYLTSIASSPV 131
LNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPR+ASASLILITSPAVYLTSI SSPV
Sbjct: 64 LNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRIASASLILITSPAVYLTSIVSSPV 123
Query: 132 SFLLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGATQLIMPYVFAI 191
+F L RFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGATQLIMPYVFAI
Sbjct: 124 TFFLARFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGATQLIMPYVFAI 183
Query: 192 IEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTGEKAKDKLSKVV 251
IEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNF RL+KTGEKAKDK SKVV
Sbjct: 184 IEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFGRLKKTGEKAKDKFSKVV 243
Query: 252 YNGVRNYRGWILALTYGYSFGVELTIDNVIANYFYDRFNLNLHTAGIVAASFGLANLFSR 311
YNGV NYRGWILALTYGY FGVELTIDNVIANYFYDRFNLNLHTAGIVAASFGLANLFSR
Sbjct: 244 YNGVTNYRGWILALTYGYCFGVELTIDNVIANYFYDRFNLNLHTAGIVAASFGLANLFSR 303
Query: 312 PYGGVLSDVVGKRYGMRGRLWALWLVQTIGGILCVILGLVNSLSASIAIMIVFSVFVQAA 371
PYGGVLSDVV KR+GMRGRLWALWLVQTIGGILCV+LGLV+SLS+SIA+M++FSVFVQAA
Sbjct: 304 PYGGVLSDVVAKRFGMRGRLWALWLVQTIGGILCVVLGLVDSLSSSIAVMLIFSVFVQAA 363
Query: 372 CGLTFGVVPFVSRSML 388
CGLTFGVVPFVSR L
Sbjct: 364 CGLTFGVVPFVSRRSL 379
BLAST of Lsi04G005680 vs. NCBI nr
Match:
gi|590694851|ref|XP_007044725.1| (Nitrate transporter2.5 [Theobroma cacao])
HSP 1 Score: 608.2 bits (1567), Expect = 1.1e-170
Identity = 301/374 (80.48%), Postives = 340/374 (90.91%), Query Frame = 1
Query: 14 QPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPLLPVIRDNLN 73
QP+KF+LPVDSEHK+T+F +FSVAAPHMR FHLSWISF ACF+S+FAA PLLP+IRDNLN
Sbjct: 11 QPQKFALPVDSEHKATEFRLFSVAAPHMRAFHLSWISFFACFVSTFAAPPLLPIIRDNLN 70
Query: 74 LTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPAVYLTSIASSPVSF 133
LT TDIGNAG+ASVSGAVFARI MG+ACDL GPRLASASLIL+T+PAVY TSIASSPVSF
Sbjct: 71 LTATDIGNAGVASVSGAVFARIAMGTACDLFGPRLASASLILLTAPAVYFTSIASSPVSF 130
Query: 134 LLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGATQLIMPYVFAIIE 193
LLVRFFTGFSLATFVSTQFWMSSMFS+ VVGTANGVA GWGNLGGGATQLIMP VF++I
Sbjct: 131 LLVRFFTGFSLATFVSTQFWMSSMFSTPVVGTANGVAAGWGNLGGGATQLIMPLVFSVIR 190
Query: 194 HIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTGEKAKDKLSKVVYN 253
IGA KFTAWRIAFFIPALFQTL+AFA+L+ G+D PDGN++RLQK+G K KDK S+V Y+
Sbjct: 191 DIGAVKFTAWRIAFFIPALFQTLAAFAILIFGQDLPDGNYQRLQKSGTKQKDKFSRVFYH 250
Query: 254 GVRNYRGWILALTYGYSFGVELTIDNVIANYFYDRFNLNLHTAGIVAASFGLANLFSRPY 313
G+ NYRGWILALTYGY FGVELT+DN+IA YFYDRFNL LHTAGI+AASFGLANLFSRP
Sbjct: 251 GITNYRGWILALTYGYCFGVELTVDNIIAEYFYDRFNLKLHTAGIIAASFGLANLFSRPA 310
Query: 314 GGVLSDVVGKRYGMRGRLWALWLVQTIGGILCVILGLVNSLSASIAIMIVFSVFVQAACG 373
GG++SD + R+GMRGRLWALW++QT+GG+ C+ILG V SLSASI +MI+FSVFVQAACG
Sbjct: 311 GGIISDRMASRFGMRGRLWALWIIQTLGGVFCIILGQVGSLSASIIVMIIFSVFVQAACG 370
Query: 374 LTFGVVPFVSRSML 388
LTFGVVPFVSR L
Sbjct: 371 LTFGVVPFVSRRSL 384
BLAST of Lsi04G005680 vs. NCBI nr
Match:
gi|566206749|ref|XP_002321622.2| (high-affinity nitrate transporter family protein [Populus trichocarpa])
HSP 1 Score: 602.4 bits (1552), Expect = 6.3e-169
Identity = 303/387 (78.29%), Postives = 342/387 (88.37%), Query Frame = 1
Query: 1 MEVDGGATAAAAGQPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFA 60
ME++G AT + QP KF+LPVDSEHK+T+F +FSVAAPHMR FHLSW+SF ACF+S+FA
Sbjct: 1 MEIEGQATVKES-QPPKFALPVDSEHKATEFRLFSVAAPHMRAFHLSWVSFFACFVSTFA 60
Query: 61 AAPLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPA 120
A PLLP+IRDNLNLT +DIGNAGIASVSGAVFAR+ MG+ACDL GPRLASASLILIT+PA
Sbjct: 61 APPLLPIIRDNLNLTASDIGNAGIASVSGAVFARVAMGTACDLFGPRLASASLILITAPA 120
Query: 121 VYLTSIASSPVSFLLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGA 180
VY TSIASS SFLLVRFFTGFSLATFVSTQFWMSSMFS+ VVGTANGVAGGWGNLGGGA
Sbjct: 121 VYFTSIASSSTSFLLVRFFTGFSLATFVSTQFWMSSMFSAPVVGTANGVAGGWGNLGGGA 180
Query: 181 TQLIMPYVFAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTG 240
TQLIMP VF +I IGA KFTAWRIAFFIPALFQTLSAFAVL+ G+D PDGNF RLQK G
Sbjct: 181 TQLIMPLVFGLIRDIGAIKFTAWRIAFFIPALFQTLSAFAVLIFGKDLPDGNFGRLQKAG 240
Query: 241 EKAKDKLSKVVYNGVRNYRGWILALTYGYSFGVELTIDNVIANYFYDRFNLNLHTAGIVA 300
+K KDK S V Y+G++NYRGWILAL+YGY FGVELTIDN++A YFYDRF+L LHTAG++A
Sbjct: 241 DKTKDKFSNVFYHGIKNYRGWILALSYGYCFGVELTIDNIVAEYFYDRFDLKLHTAGMIA 300
Query: 301 ASFGLANLFSRPYGGVLSDVVGKRYGMRGRLWALWLVQTIGGILCVILGLVNSLSASIAI 360
ASFGLAN+ SRP GG++SD VGKR+GMRGRLWALW+ QT+GG+ C+ILG V SL ASI +
Sbjct: 301 ASFGLANIVSRPGGGMISDAVGKRFGMRGRLWALWIAQTLGGVFCIILGRVGSLGASIVV 360
Query: 361 MIVFSVFVQAACGLTFGVVPFVSRSML 388
MIVFS+F QAACGLTFGVVPFVSR L
Sbjct: 361 MIVFSLFCQAACGLTFGVVPFVSRRSL 386
BLAST of Lsi04G005680 vs. NCBI nr
Match:
gi|57283317|emb|CAG26717.1| (nitrate transporter [Populus tremula x Populus tremuloides])
HSP 1 Score: 602.1 bits (1551), Expect = 8.2e-169
Identity = 302/387 (78.04%), Postives = 343/387 (88.63%), Query Frame = 1
Query: 1 MEVDGGATAAAAGQPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFA 60
ME++G AT + QP KF+LPVDSEHK+T+F +FSVAAPHMR FHLSW+SF ACF+SSFA
Sbjct: 1 MEIEGQATVKES-QPPKFALPVDSEHKATEFRLFSVAAPHMRAFHLSWVSFFACFVSSFA 60
Query: 61 AAPLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPA 120
A PLLP+IRDNLNLT +DIGNAGIASVSGAVFAR+ MG+ACDL GPRLASASLIL+T+PA
Sbjct: 61 APPLLPIIRDNLNLTASDIGNAGIASVSGAVFARVAMGTACDLFGPRLASASLILLTAPA 120
Query: 121 VYLTSIASSPVSFLLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGA 180
VY TS+ASS SFLLVRFFTGFSLATFVSTQFWMSSMFS+ VVGTANGVAGGWGNLGGGA
Sbjct: 121 VYFTSMASSSTSFLLVRFFTGFSLATFVSTQFWMSSMFSAPVVGTANGVAGGWGNLGGGA 180
Query: 181 TQLIMPYVFAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTG 240
TQLIMP VF +I IGA KFTAWRIAFFIPALFQTLSAFAVL+ G+D PDGNFRRLQK G
Sbjct: 181 TQLIMPLVFGLIRDIGAIKFTAWRIAFFIPALFQTLSAFAVLIFGKDLPDGNFRRLQKAG 240
Query: 241 EKAKDKLSKVVYNGVRNYRGWILALTYGYSFGVELTIDNVIANYFYDRFNLNLHTAGIVA 300
+K KDK S V Y+G++NYRGWILAL+YGY FGVELTIDN++A YFYDRF+L LHTAG++A
Sbjct: 241 DKTKDKFSNVFYHGIKNYRGWILALSYGYCFGVELTIDNIVAEYFYDRFDLKLHTAGMIA 300
Query: 301 ASFGLANLFSRPYGGVLSDVVGKRYGMRGRLWALWLVQTIGGILCVILGLVNSLSASIAI 360
ASFGLAN+ SRP GG++SD VGKR+GMRG+LWALW+VQT+GG+ C+ILG V SL ASI +
Sbjct: 301 ASFGLANIVSRPGGGMISDAVGKRFGMRGKLWALWVVQTLGGVFCIILGRVGSLGASIVV 360
Query: 361 MIVFSVFVQAACGLTFGVVPFVSRSML 388
MIVF +F QAACGLTFGVVPFVSR L
Sbjct: 361 MIVFFLFCQAACGLTFGVVPFVSRRSL 386
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| NRT25_ARATH | 1.8e-155 | 70.41 | High affinity nitrate transporter 2.5 OS=Arabidopsis thaliana GN=NRT2.5 PE=2 SV=... | [more] |
| NRT23_ORYSJ | 8.8e-147 | 68.81 | High-affinity nitrate transporter 2.3 OS=Oryza sativa subsp. japonica GN=NRT2.3 ... | [more] |
| NRT21_ARATH | 5.4e-128 | 59.30 | High-affinity nitrate transporter 2.1 OS=Arabidopsis thaliana GN=NRT2.1 PE=1 SV=... | [more] |
| NRT22_ARATH | 2.7e-127 | 58.60 | High-affinity nitrate transporter 2.2 OS=Arabidopsis thaliana GN=NRT2.2 PE=2 SV=... | [more] |
| NRT21_ORYSJ | 2.9e-126 | 57.96 | High-affinity nitrate transporter 2.1 OS=Oryza sativa subsp. japonica GN=NRT2.1 ... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| S4VIV8_CUCSA | 3.0e-194 | 93.88 | High-affinity nitrate transporter NRT2.3 OS=Cucumis sativus GN=NRT2.3 PE=2 SV=1 | [more] |
| A0A061E862_THECC | 7.9e-171 | 80.48 | Nitrate transporter2.5 OS=Theobroma cacao GN=TCM_010451 PE=4 SV=1 | [more] |
| B9IEW2_POPTR | 4.4e-169 | 78.29 | High-affinity nitrate transporter family protein OS=Populus trichocarpa GN=POPTR... | [more] |
| Q5K409_9ROSI | 5.7e-169 | 78.04 | Nitrate transporter OS=Populus tremula x Populus tremuloides GN=tNit2.2 PE=2 SV=... | [more] |
| V7C8S4_PHAVU | 7.4e-169 | 76.49 | Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_003G062800g PE=4 SV=1 | [more] |
