Lsi04G000990 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi04G000990
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Major facilitator superfamily protein, putative
Location	chr04 : 1126070 .. 1128408 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGGATTCAACCTGAAATCCTTTACACTAAATCTAATCAAAGGGTCATGGTTCATGATGTTTGCGTCATTCCTAATCATGTCGATGGCAGGCATTCCGTACATGTTCGGCTTATACTCGAGCACCATAAAATCTGTGTTAGGGTACGACCAAACAACCTTAAACTTCATAAGTTTCTTCAAAGACGTCGGAACGACGGTCGGTGTTGTAGCGGGATTAATCAACGAAGTAACCCCGCCGTGGTCCATTCTAGCCATGGGAGCTGTTCTCAATTTCTTTGGCTTCTTCATGATTTGGTTATCCGTCTCCGAAAAAATCCCAACTCACGTTTGGTTAATGTGCCTCTACATCTGCATCGGTGCCAACGCCACGACGTTCGCCAACACCGGCGCGTTAGTTACGTGCGTAAAAAACTACCCGCAACGGCGAGGCGTCGTGATTGGGATTTTGAAAGGGTATATGGGATTGAGCGGGGCAATTGTGACGCAATTGTATCATGCCATATATGGGATAGATGAGAAGTCGTTGATATTATTGTTGGGGTGGTTGCCAGCGGCGGTGTCGTTGGTGTTTATGCCGACAGTGCGGCGGATGAAGGTGGAGCATGAGGAGGATGAGTTGAAAGTTTTTTATAGGTTTTTGTATATATCGTTGGGATTGGCGGGATTTTTGATGGTGATGATAATTTTGCAACAGAAGTTTAATTTTAAGAGAGAGGAATATGGAGGGAGTGCGGTGGTTGTTACTTTTTTGTTGCTTTTGCCTATTGCTATTGTTGTTGCTCAAAGAGTTTAAGTCATGGCGGCGGTTGAATAAGCCGGCGGTTCTTGAAAATGGTCTGTCTCCCTTGCCGGTGAGTGTAAAATGGACCTTAATTCTCTTTTAAATATTTATTTATCTATTTGATACCTTTTACTAGTTGTATGTCAATTTTTTTAGAAATTCTTTTAATCATTTTATTGATTTTGATTTTAAAAAGAAAAATATTTTGTATATCCAGAGCCGCACTCATCTTTATGCAATTTATTTTTTTTTAAAAAAAAATACTTTTTATATTATTTTTCTTTTTGAATTGAAGGAGAGGTTGCACATAAGTATTGCTCTTTGAAAAATACTTAAACAAGTCTTTGGAGCAAAGAGTGGAGTTGTGAATTCTAAAGAATAGTGAACCGACAATGTTTTTTCTTTTTTAAAATATATTTATATTTTAATATATTGGAGTAAGCTTGTGCAAGATGGACCAAGTATTATTGCTCTTTTGATTTTTTGAATGGGGAAAGAGATGATTGTTTGTAGGGTCTCTATCTTAATTTTAAAATACTTATCATGAACAGAAAACATACATAGGATGAGAGATTAATTTTGAACTATACGTTGAAATCTTCATATCAAAATTTATTTGTTAATTTTACCATTTTATCTTCATCCAAATTGTTAAAATTGATGATTTCCGACCTGACTAATTAACATGATTTTAATAATAGGGGTCACCACCATTGAAGAACACAGTACCAATGTCACTCATCCCAAAGAAACCAAAACCCCAACCACAACAACCCATCAAAGTCAAGTGGTGGAAAAACGTATTCAATCCCCCACCAAGAGGAGATGATTGGACAATCCTCCAAGCCCTTTTTAGCTTCGACATGTTCCTCCTCTTCCTGGCCACCGCCTGCGGCGTCGGCGGCACTCTCACCGCCATCGACAACCTCGGCCAAATCGGCCAATCCCAAAATTACCCCAAAAAAAGCATCAGCACATTCGTTTCCCTCGTCAGCATCTGGAATTACCTCGGTCGAGTAATGGCTGGATTCCTCTCCGAACATTTACTCATCAAATACAAATTCCCTCGTCCTTTAATGCTCACAATCGTCCTTCTTCTTTCATGCATTGCCCATCTTTTAATCGCCTTCAATCCCGCTGGCGGCCTTTACATTGCCTCTGTTATGACCGGATTCTGTTACGGCGCTCAATGGCCTCTGCTTTTTGCCATTGTTTCTGAGATTTTTGGGCTGAAATATTATGCGACTCTGTATAATTTCGGGTCGGTGGCTAGCCCAGTAGGGCTTTATGTGTTGAATGTGAATGTGGCTGGGTATTTGTATGATAAGGAAGCTAAGAAGCAGCTGGCGATGGCGGGGAGGATTAGGAAAACAGGGGAGGAGTTGATCTGTCATGGGACGGTGTGTTTTAAGCTGTCTTTTGTGATTATTACGGCAGTGAGTTTGTTTGGGGCTTTAGTTTCTTTGGTTTTGGTGTTGAGGACAAAGAAGTTTTATAAGAGTGATATTTATCGGAAATTTAGGGAGACAGAGGAGGCGGCGGCGGTGGCGTAG

mRNA sequence

ATGGAGGGATTCAACCTGAAATCCTTTACACTAAATCTAATCAAAGGGTCATGGTTCATGATGTTTGCGTCATTCCTAATCATGTCGATGGCAGGCATTCCGTACATGTTCGGCTTATACTCGAGCACCATAAAATCTGTGTTAGGGTACGACCAAACAACCTTAAACTTCATAAGTTTCTTCAAAGACGTCGGAACGACGGTCGGTGTTGTAGCGGGATTAATCAACGAAGTAACCCCGCCGTGGTCCATTCTAGCCATGGGAGCTGTTCTCAATTTCTTTGGCTTCTTCATGATTTGGTTATCCGTCTCCGAAAAAATCCCAACTCACGTTTGGTTAATGTGCCTCTACATCTGCATCGGTGCCAACGCCACGACGTTCGCCAACACCGGCGCGTTAGTTACGTGCGTAAAAAACTACCCGCAACGGCGAGGCGTCGTGATTGGGATTTTGAAAGGGTATATGGGATTGAGCGGGGCAATTGTGACGCAATTGTATCATGCCATATATGGGATAGATGAGAAGTCGTTGATATTATTGTTGGGGTGGTTGCCAGCGGCGGTGTCGTTGGTGTTTATGCCGACAGTGCGGCGGATGAAGGTGGAGCATGAGGAGGATGAGTTGAAAGGGTCACCACCATTGAAGAACACAGTACCAATGTCACTCATCCCAAAGAAACCAAAACCCCAACCACAACAACCCATCAAAGTCAAGTGGTGGAAAAACGTATTCAATCCCCCACCAAGAGGAGATGATTGGACAATCCTCCAAGCCCTTTTTAGCTTCGACATGTTCCTCCTCTTCCTGGCCACCGCCTGCGGCGTCGGCGGCACTCTCACCGCCATCGACAACCTCGGCCAAATCGGCCAATCCCAAAATTACCCCAAAAAAAGCATCAGCACATTCGTTTCCCTCGTCAGCATCTGGAATTACCTCGGTCGAGTAATGGCTGGATTCCTCTCCGAACATTTACTCATCAAATACAAATTCCCTCGTCCTTTAATGCTCACAATCGTCCTTCTTCTTTCATGCATTGCCCATCTTTTAATCGCCTTCAATCCCGCTGGCGGCCTTTACATTGCCTCTGTTATGACCGGATTCTGTTACGGCGCTCAATGGCCTCTGCTTTTTGCCATTGTTTCTGAGATTTTTGGGCTGAAATATTATGCGACTCTGTATAATTTCGGGTCGGTGGCTAGCCCAGTAGGGCTTTATGTGTTGAATGTGAATGTGGCTGGGTATTTGTATGATAAGGAAGCTAAGAAGCAGCTGGCGATGGCGGGGAGGATTAGGAAAACAGGGGAGGAGTTGATCTGTCATGGGACGGTGTGTTTTAAGCTGTCTTTTGTGATTATTACGGCAGTGAGTTTGTTTGGGGCTTTAGTTTCTTTGGTTTTGGTGTTGAGGACAAAGAAGTTTTATAAGAGTGATATTTATCGGAAATTTAGGGAGACAGAGGAGGCGGCGGCGGTGGCGTAG

Coding sequence (CDS)

ATGGAGGGATTCAACCTGAAATCCTTTACACTAAATCTAATCAAAGGGTCATGGTTCATGATGTTTGCGTCATTCCTAATCATGTCGATGGCAGGCATTCCGTACATGTTCGGCTTATACTCGAGCACCATAAAATCTGTGTTAGGGTACGACCAAACAACCTTAAACTTCATAAGTTTCTTCAAAGACGTCGGAACGACGGTCGGTGTTGTAGCGGGATTAATCAACGAAGTAACCCCGCCGTGGTCCATTCTAGCCATGGGAGCTGTTCTCAATTTCTTTGGCTTCTTCATGATTTGGTTATCCGTCTCCGAAAAAATCCCAACTCACGTTTGGTTAATGTGCCTCTACATCTGCATCGGTGCCAACGCCACGACGTTCGCCAACACCGGCGCGTTAGTTACGTGCGTAAAAAACTACCCGCAACGGCGAGGCGTCGTGATTGGGATTTTGAAAGGGTATATGGGATTGAGCGGGGCAATTGTGACGCAATTGTATCATGCCATATATGGGATAGATGAGAAGTCGTTGATATTATTGTTGGGGTGGTTGCCAGCGGCGGTGTCGTTGGTGTTTATGCCGACAGTGCGGCGGATGAAGGTGGAGCATGAGGAGGATGAGTTGAAAGGGTCACCACCATTGAAGAACACAGTACCAATGTCACTCATCCCAAAGAAACCAAAACCCCAACCACAACAACCCATCAAAGTCAAGTGGTGGAAAAACGTATTCAATCCCCCACCAAGAGGAGATGATTGGACAATCCTCCAAGCCCTTTTTAGCTTCGACATGTTCCTCCTCTTCCTGGCCACCGCCTGCGGCGTCGGCGGCACTCTCACCGCCATCGACAACCTCGGCCAAATCGGCCAATCCCAAAATTACCCCAAAAAAAGCATCAGCACATTCGTTTCCCTCGTCAGCATCTGGAATTACCTCGGTCGAGTAATGGCTGGATTCCTCTCCGAACATTTACTCATCAAATACAAATTCCCTCGTCCTTTAATGCTCACAATCGTCCTTCTTCTTTCATGCATTGCCCATCTTTTAATCGCCTTCAATCCCGCTGGCGGCCTTTACATTGCCTCTGTTATGACCGGATTCTGTTACGGCGCTCAATGGCCTCTGCTTTTTGCCATTGTTTCTGAGATTTTTGGGCTGAAATATTATGCGACTCTGTATAATTTCGGGTCGGTGGCTAGCCCAGTAGGGCTTTATGTGTTGAATGTGAATGTGGCTGGGTATTTGTATGATAAGGAAGCTAAGAAGCAGCTGGCGATGGCGGGGAGGATTAGGAAAACAGGGGAGGAGTTGATCTGTCATGGGACGGTGTGTTTTAAGCTGTCTTTTGTGATTATTACGGCAGTGAGTTTGTTTGGGGCTTTAGTTTCTTTGGTTTTGGTGTTGAGGACAAAGAAGTTTTATAAGAGTGATATTTATCGGAAATTTAGGGAGACAGAGGAGGCGGCGGCGGTGGCGTAG

Protein sequence

MEGFNLKSFTLNLIKGSWFMMFASFLIMSMAGIPYMFGLYSSTIKSVLGYDQTTLNFISFFKDVGTTVGVVAGLINEVTPPWSILAMGAVLNFFGFFMIWLSVSEKIPTHVWLMCLYICIGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGIDEKSLILLLGWLPAAVSLVFMPTVRRMKVEHEEDELKGSPPLKNTVPMSLIPKKPKPQPQQPIKVKWWKNVFNPPPRGDDWTILQALFSFDMFLLFLATACGVGGTLTAIDNLGQIGQSQNYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYKFPRPLMLTIVLLLSCIAHLLIAFNPAGGLYIASVMTGFCYGAQWPLLFAIVSEIFGLKYYATLYNFGSVASPVGLYVLNVNVAGYLYDKEAKKQLAMAGRIRKTGEELICHGTVCFKLSFVIITAVSLFGALVSLVLVLRTKKFYKSDIYRKFRETEEAAAVA

Homology

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BLAST of Lsi04G000990 vs. Swiss-Prot
Match: NFD4_ARATH (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana GN=NFD4 PE=3 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 4.7e-08
Identity = 55/227 (24.23%), Postives = 105/227 (46.26%), Query Frame = 1


Query: 250 GDDWTILQALFSFDMFLLFLATACGVGGTLTAIDNLGQIGQSQNYPKKSISTFVSLVSIW 309
           GD+ +    +   + +L ++A  CG    L   +NLGQI QS     ++ +T V++ S +
Sbjct: 346 GDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLG---QNSTTLVTIYSSF 405

Query: 310 NYLGRVMAGFLSEHLLIKYKFPRPLMLTIVLLLSCIAHLLIAFNPAG--GLYIASVMTGF 369
           ++ GR+++    + +  +++  R     I LL + IA  L+A + +    L  A+ + G 
Sbjct: 406 SFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTALQTATALIGL 465

Query: 370 CYGAQWPLLFAIVSEIFGLKYYATLYNFGSVASPVGLYVLNVNVAGYLYDKEAKKQLAMA 429
             G  +    +I S++FG       +N      P+G  +    +A  +Y+  A   +   
Sbjct: 466 SSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGY-IAASIYEANASPDITPI 525

Query: 430 GRIRKTGEELICHGTVCFKLSFVIITAVSLFGALVSLVLVLRTKKFY 475
                  + ++C G  C+  +FV    +S+ G + SL L +RTK  Y
Sbjct: 526 -----VSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

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BLAST of Lsi04G000990 vs. TrEMBL
Match: A0A0R0HXG0_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_10G058800 PE=4 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 7.0e-152
Identity = 285/499 (57.11%), Postives = 365/499 (73.15%), Query Frame = 1


Query: 8   SFTLNLIKGSWFMMFASFLIMSMAGIPYMFGLYSSTIKSVLGYDQTTLNFISFFKDVGTT 67
           +F  ++I G WFM+FAS LIM+++G  YMFGLYS+ +K+ LGYDQ+TLN ISFFKD+G  
Sbjct: 5   AFIHHVITGRWFMIFASCLIMAVSGATYMFGLYSNEVKASLGYDQSTLNLISFFKDLGAN 64

Query: 68  VGVVAGLINEVTPPWSILAMGAVLNFFGFFMIWLSVSEKIPT-HVWLMCLYICIGANATT 127
           +G+ +GLINE++PPW ILAMGA +NF G+FMIWLSV+ +I    VW MCLY  IGAN+ +
Sbjct: 65  LGIFSGLINEISPPWVILAMGATMNFIGYFMIWLSVTSRIAKPQVWQMCLYFYIGANSQS 124

Query: 128 FANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYG--------------- 187
           FANTGALV CVK++P+ RG VIG+LKGY+GLSGAI TQ YHA YG               
Sbjct: 125 FANTGALVNCVKSFPRSRGSVIGLLKGYVGLSGAIFTQFYHAFYGFLMVLIVVQNKLSFT 184

Query: 188 ----IDEKSLILLLGWLPAAVSLVFMPTVRRMKVEHEEDELKGSPPLKNTVPMSLIPKKP 247
               I +  ++LLL  LP  + +VF    +  K +++      +    + V +S  P++ 
Sbjct: 185 RVEFIVDGMVVLLLLLLP--LGIVFKEEFKIWKNQNQNF----TDAAASVVELSQ-PEEA 244

Query: 248 KPQPQQPIKVKWWKNVFNPPPRGDDWTILQALFSFDMFLLFLATACGVGGTLTAIDNLGQ 307
               ++       KNVF PP RG+D+TI QALFS DM +LF+AT  GVGGTLTA+DNLGQ
Sbjct: 245 PSHSERKNNNSCLKNVFKPPKRGEDYTIFQALFSIDMLILFIATVFGVGGTLTALDNLGQ 304

Query: 308 IGQSQNYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYKFPRPLMLTIVLLLSCIAH 367
           IG S  YPKKS++TFVSLVSIWNYLGR  +GF+SE+LL KYKFPRPL+LT+V+LLSC+ H
Sbjct: 305 IGNSLGYPKKSLTTFVSLVSIWNYLGRASSGFVSEYLLTKYKFPRPLLLTLVMLLSCVGH 364

Query: 368 LLIAFNPAGGLYIASVMTGFCYGAQWPLLFAIVSEIFGLKYYATLYNFGSVASPVGLYVL 427
           +LIAF     LY +SV+ GFC+GA WPL+FAI+SEIFGLKYY+TLYNFG+VASPVG Y+L
Sbjct: 365 ILIAFGIPNSLYFSSVIIGFCFGAIWPLMFAIISEIFGLKYYSTLYNFGAVASPVGSYIL 424

Query: 428 NVNVAGYLYDKEAKKQLAMAGRIRKTGEELICHGTVCFKLSFVIITAVSLFGALVSLVLV 487
           NV V GYLYDKEA KQL + G IR+ G++L C G  C++++F+IITA +L G +VS +LV
Sbjct: 425 NVRVTGYLYDKEALKQLGVKGLIRQKGKDLTCVGVQCYRMAFLIITASTLVGCVVSFILV 484

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BLAST of Lsi04G000990 vs. TrEMBL
Match: A0A0D2TCP2_GOSRA (Uncharacterized protein (Fragment) OS=Gossypium raimondii GN=B456_011G234900 PE=4 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 1.0e-147
Identity = 272/489 (55.62%), Postives = 363/489 (74.23%), Query Frame = 1


Query: 3   GFNLKSFTLNLIKGSWFMMFASFLIMSMAGIPYMFGLYSSTIKSVLGYDQTTLNFISFFK 62
           G+    F L +I G WFMMFASFLIM+ AG  Y+FG+YS  IKS LGYDQ+TLN +S FK
Sbjct: 16  GYVCCRFFLQVITGRWFMMFASFLIMACAGATYLFGVYSKDIKSTLGYDQSTLNLMSTFK 75

Query: 63  DVGTTVGVVAGLINEVTPPWSILAMGAVLNFFGFFMIWLSVSEKIPT-HVWLMCLYICIG 122
           D+G  VGV++GL+ EVTP W +L +GA++NF G+FMIWL+V++++ T  VW MC YICIG
Sbjct: 76  DLGANVGVLSGLLAEVTPTWFVLLVGALMNFTGYFMIWLAVTKRVATPKVWQMCAYICIG 135

Query: 123 ANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGIDEKSLILLL 182
           AN+  FANTG+LVTCVKN+PQ RG+++G++KG++GLSGAI  Q+Y+AIYG D KSLILL+
Sbjct: 136 ANSQNFANTGSLVTCVKNFPQSRGMMLGLMKGFVGLSGAIFPQIYYAIYGNDSKSLILLI 195

Query: 183 GWLPAAVSLVFMPTVRRMKVEHEEDELK------GSPPLKNTVPMSLIPKKPKPQPQQPI 242
           GW PAA+SLVF+  +R MK+    +E K      G   +   + M+L   + K +     
Sbjct: 196 GWFPAAISLVFVYNIRAMKISTHPNETKVLHEYLGIATVLALLIMALTIAQKKTKEAS-- 255

Query: 243 KVKWWKNVFNPPPRGDDWTILQALFSFDMFLLFLATACGVGGTLTAIDNLGQIGQSQNYP 302
                 ++F PP RG+D++ILQALFS DMFL+FL T CG+G +LTA+DNLGQIG+S  YP
Sbjct: 256 -ASGCSSIFKPPKRGEDFSILQALFSIDMFLVFLGTFCGLGCSLTAVDNLGQIGESLGYP 315

Query: 303 KKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYKFPRPLMLTIVLLLSCIAHLLIAFNPA 362
            +++ST VSL+SIWNY GRV AGF+SE +L+KYK PRPL++++V LL+ +  LLIAF   
Sbjct: 316 YQAVSTVVSLLSIWNYFGRVFAGFVSEIILLKYKIPRPLIMSLVFLLAAVGDLLIAFPWP 375

Query: 363 GGLYIASVMTGFCYGAQWPLLFAIVSEIFGLKYYATLYNFGSVASPVGLYVLNVNVAGYL 422
           G +Y+AS++ GF +GAQ  LLF IVSE+FGLKYYATL+N   ++SP+G +VLNV + G L
Sbjct: 376 GSVYLASLLLGFSFGAQQTLLFTIVSELFGLKYYATLFNCAQLSSPLGSFVLNVRIVGKL 435

Query: 423 YDKEAKKQLAMAGRIRKTGEELICHGTVCFKLSFVIITAVSLFGALVSLVLVLRTKKFYK 482
           YDKEA KQLA  G  R   +EL C G  C++LSF I+ A + FG++V LVLVLRT+K+Y 
Sbjct: 436 YDKEAWKQLAAKGMARSMVKELTCIGEQCYRLSFSILAATNTFGSVVILVLVLRTRKYYG 495

Query: 483 SDIYRKFRE 485
            DIY++FR+
Sbjct: 496 GDIYKRFRD 501

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BLAST of Lsi04G000990 vs. TrEMBL
Match: A0A0A0KWQ1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G622530 PE=4 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 4.8e-145
Identity = 259/281 (92.17%), Postives = 275/281 (97.86%), Query Frame = 1


Query: 210 GSPPLKNTVPMSLIPKKPKPQPQQPIKVKWWKNVFNPPPRGDDWTILQALFSFDMFLLFL 269
           GSPPLKNT P+SL+PKKPK Q Q+PIK +WWKNVFNPPPRGDDWTILQALFSFDMFLLFL
Sbjct: 132 GSPPLKNTTPISLLPKKPKSQQQEPIKTEWWKNVFNPPPRGDDWTILQALFSFDMFLLFL 191

Query: 270 ATACGVGGTLTAIDNLGQIGQSQNYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYK 329
           ATACGVGGTLTAIDNL QIGQSQ+YPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYK
Sbjct: 192 ATACGVGGTLTAIDNLAQIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYK 251

Query: 330 FPRPLMLTIVLLLSCIAHLLIAFNPAGGLYIASVMTGFCYGAQWPLLFAIVSEIFGLKYY 389
           FPRPLMLTIVLLLSCIAHLLIAFNP+GGLYIAS++TG+CYGAQWPLLFAIVSEIFGLKYY
Sbjct: 252 FPRPLMLTIVLLLSCIAHLLIAFNPSGGLYIASILTGYCYGAQWPLLFAIVSEIFGLKYY 311

Query: 390 ATLYNFGSVASPVGLYVLNVNVAGYLYDKEAKKQLAMAGRIRKTGEELICHGTVCFKLSF 449
           ATLYNFGSVASPVGLY+LNVNVAGYLYDKEAKKQL+MAG+IRKTGEEL+C+GTVCFKLSF
Sbjct: 312 ATLYNFGSVASPVGLYLLNVNVAGYLYDKEAKKQLSMAGKIRKTGEELVCNGTVCFKLSF 371

Query: 450 VIITAVSLFGALVSLVLVLRTKKFYKSDIYRKFRETEEAAA 491
           VIITAVSLFGALVSLVLVLRTKKFYKSDIY+KF+E EEAAA
Sbjct: 372 VIITAVSLFGALVSLVLVLRTKKFYKSDIYKKFKEAEEAAA 412

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BLAST of Lsi04G000990 vs. TrEMBL
Match: A0A061EU62_THECC (Major facilitator superfamily protein, putative OS=Theobroma cacao GN=TCM_022284 PE=4 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 8.2e-145
Identity = 275/508 (54.13%), Postives = 361/508 (71.06%), Query Frame = 1


Query: 6   LKSFTLNLIKGSWFMMFASFLIMSMAGIPYMFGLYSSTIKSVLGYDQTTLNFISFFKDVG 65
           +KSF L ++    FM+FA+ +IMS+ G  YMFGLYS+ IK+ LGYDQTTLN +SFFKD+G
Sbjct: 15  MKSFFLAVLSSRLFMVFATIIIMSVNGSGYMFGLYSNDIKTTLGYDQTTLNLLSFFKDLG 74

Query: 66  TTVGVVAGLINEVTPPWSILAMGAVLNFFGFFMIWLSVSEKIPT-HVWLMCLYICIGANA 125
             +G+++GLI EV PPW +L+MG+VLNFFG+FMIWLSV+ +     VW MCLY+ I AN+
Sbjct: 75  GNLGILSGLIYEVVPPWVVLSMGSVLNFFGYFMIWLSVTSRTAKPQVWQMCLYMWIAANS 134

Query: 126 TTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGIDEKSLILLL--- 185
            +F NTGALVTCVKN+P  RG V+G+LKG++GLSGAI+TQ  +   G+    +++++   
Sbjct: 135 QSFPNTGALVTCVKNFPGSRGCVLGLLKGFIGLSGAIMTQFLYIALGLAGFIMVMIILQN 194

Query: 186 ----------GWLPAAVSLVFMPTVRRMKVEHEEDELKGSPPLKNTVPMSLIPKKPKPQP 245
                     G     + L+ +P V  +K E      K      ++  ++++ + P   P
Sbjct: 195 RLSFTRTEYIGSASVVLILLLLPLVIVIKEEFNVSTSKTQASNTHS-QLNVVIENPPAVP 254

Query: 246 QQPIKV-----KWWKNVFNPPPRGDDWTILQALFSFDMFLLFLATACGVGGTLTAIDNLG 305
            +P +         K +F PP RG+D+TILQA+FS DM +LF+ATACG GG LT IDNLG
Sbjct: 255 AKPAEATKEAFNCIKKMFKPPDRGEDYTILQAIFSIDMLILFIATACGTGGALTVIDNLG 314

Query: 306 QIGQSQNYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYKFPRPLMLTIVLLLSCIA 365
           Q+G+S  YP  SIS F+SLVSIWN+LGRV+AG+LSE +L KY FPRPLMLT V+LLSC+ 
Sbjct: 315 QLGKSLGYPTHSISAFISLVSIWNFLGRVLAGYLSEIVLTKYNFPRPLMLTFVILLSCVG 374

Query: 366 HLLIAFNPAGGLYIASVMTGFCYGAQWPLLFAIVSEIFGLKYYATLYNFGSVASPVGLYV 425
           HLLIAF     LY ASVMTGFC GAQ PLL AI+SE+FGLK+Y+T+YN GSV+SPVG Y+
Sbjct: 375 HLLIAFAVPNSLYFASVMTGFCLGAQLPLLCAIISELFGLKHYSTMYNVGSVSSPVGSYI 434

Query: 426 LNVNVAGYLYDKEAKKQLAMAGRIRKTGEELICHGTVCFKLSFVIITAVSLFGALVSLVL 485
            NV VAG+LYDKEA KQ    GR RK G++L C G  C++L+F+IITA +LFG++VSL+L
Sbjct: 435 FNVRVAGHLYDKEAVKQTEALGRTRKPGQDLNCSGAKCYRLAFLIITAATLFGSIVSLIL 494

Query: 486 VLRTKKFYKSDIYRKFRE--TEEAAAVA 493
           V RT+ FYK DIY+KF+E   E  A VA
Sbjct: 495 VFRTRTFYKGDIYKKFKEEVVESQAGVA 521

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BLAST of Lsi04G000990 vs. TrEMBL
Match: B9GP32_POPTR (Uncharacterized protein (Fragment) OS=Populus trichocarpa GN=POPTR_0002s13500g PE=4 SV=2)

HSP 1 Score: 521.2 bits (1341), Expect = 1.4e-144
Identity = 274/501 (54.69%), Postives = 357/501 (71.26%), Query Frame = 1


Query: 6   LKSFTLNLIKGSWFMMFASFLIMSMAGIPYMFGLYSSTIKSVLGYDQTTLNFISFFKDVG 65
           ++SF+L ++   WF +FAS LIMS+ G  YMFGLYS  IK+ LGYDQTTLN +SFFKD+G
Sbjct: 1   MRSFSLQILSSRWFTIFASLLIMSVNGTSYMFGLYSGDIKTSLGYDQTTLNTLSFFKDLG 60

Query: 66  TTVGVVAGLINEVTPPWSILAMGAVLNFFGFFMIWLSVSEKI-PTHVWLMCLYICIGANA 125
             +GV AGL+ E+ PPW +L++GAV+NF  +F+IW++V+ +I    +W +CLY+C+  NA
Sbjct: 61  GNLGVSAGLVYEIMPPWVVLSIGAVMNFSAYFLIWVTVTGRINKPRLWQVCLYMCLATNA 120

Query: 126 TTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGIDEKSLILLLGWL 185
            ++ NTGALVTCVKN+P+ RG VIG+LKG + LSGAI+TQLYHA YG D KSLILL+ W+
Sbjct: 121 ASYPNTGALVTCVKNFPESRGSVIGLLKGLISLSGAIMTQLYHAFYGNDSKSLILLIAWI 180

Query: 186 PAAVSLVFMPTVRRMKVEHEEDELKGSPPLKNT---VPMSLIPKKPKPQPQQPIKV---- 245
           PA V L+F+ T+R MKV  +E ELK       T   +   ++  K K    + I +    
Sbjct: 181 PAIVPLLFLRTIRIMKVVQQEKELKVFYKFLYTALGLAGFIMLNKLKFTRAEYISIVTPP 240

Query: 246 --------KWWKNVFNPPPRGDDWTILQALFSFDMFLLFLATACGVGGTLTAIDNLGQIG 305
                       ++FN P RG+D+TILQA+ S DM ++ +AT CGVGG L AIDNLGQI 
Sbjct: 241 LGGRLEPFPCIVSIFNQPDRGEDYTILQAISSIDMLIILIATTCGVGGALAAIDNLGQIA 300

Query: 306 QSQNYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYKFPRPLMLTIVLLLSCIAHLL 365
            S  Y   +I TF+SLVS+WN+LGRV+A F SE  L KYKFPRPLMLT V+L SCI H+L
Sbjct: 301 DSLGYKTHNIGTFISLVSVWNFLGRVLASFASEVALTKYKFPRPLMLTFVILFSCIGHVL 360

Query: 366 IAFNPAGGLYIASVMTGFCYGAQWPLLFAIVSEIFGLKYYATLYNFGSVASPVGLYVLNV 425
           IAF     LYI+S++ GFC GAQ PL+ AI+SEIFGLK+++TLY+ GSV+SP+G Y+ NV
Sbjct: 361 IAFGVEHSLYISSIIIGFCLGAQLPLVSAIISEIFGLKHFSTLYSVGSVSSPIGSYIFNV 420

Query: 426 NVAGYLYDKEAKKQLAMAGRIRKTGEELICHGTVCFKLSFVIITAVSLFGALVSLVLVLR 485
            VAG LYDKEA KQ+   G  R+ G+EL C G  CF+ +FVIITA +  G LVS++LV R
Sbjct: 421 KVAGNLYDKEALKQMEALGLKREAGKELNCSGVHCFRKAFVIITAATFLGFLVSIILVYR 480

Query: 486 TKKFYKSDIYRKFRETEEAAA 491
           T++FYK DIY+KF  TEEA A
Sbjct: 481 TRRFYKGDIYKKF--TEEAVA 499

[Close]

BLAST of Lsi04G000990 vs. TAIR10
Match: AT2G39210.1 (AT2G39210.1 Major facilitator superfamily protein)

HSP 1 Score: 378.3 bits (970), Expect = 7.5e-105
Identity = 198/291 (68.04%), Postives = 228/291 (78.35%), Query Frame = 1


Query: 214 LKNTVPMSLIPKKPKPQPQQ------------PIKVKW---WKNVFNPPPRGDDWTILQA 273
           L +  P++++ +KPK    +              KVK    W  VFNPP RGDD+TILQA
Sbjct: 280 LNDPAPINVVTEKPKLDSSEFKDDDGEESKEVVEKVKTPSCWTTVFNPPERGDDYTILQA 339

Query: 274 LFSFDMFLLFLATACGVGGTLTAIDNLGQIGQSQNYPKKSISTFVSLVSIWNYLGRVMAG 333
           LFS DM +LFLAT CGVGGTLTAIDNLGQIG S  YPK+S+STFVSLVSIWNY GRV++G
Sbjct: 340 LFSVDMLILFLATICGVGGTLTAIDNLGQIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSG 399

Query: 334 FLSEHLLIKYKFPRPLMLTIVLLLSCIAHLLIAFNPAGGLYIASVMTGFCYGAQWPLLFA 393
            +SE  LIKYKFPRPLMLT+VLLLSC  HLLIAFN  GGLY+ASV+ GFC+GAQWPLLFA
Sbjct: 400 VVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIAFNVPGGLYVASVIIGFCFGAQWPLLFA 459

Query: 394 IVSEIFGLKYYATLYNFGSVASPVGLYVLNVNVAGYLYDKEAKKQLAMAGRIRKTGEELI 453
           I+SEIFGLKYY+TLYNFGSVASP+G Y+LNV VAGYLYD EA KQ    G+ R  G++L 
Sbjct: 460 IISEIFGLKYYSTLYNFGSVASPIGSYLLNVRVAGYLYDVEAGKQYKALGKTRVEGQDLN 519

Query: 454 CHGTVCFKLSFVIITAVSLFGALVSLVLVLRTKKFYKSDIYRKFRETEEAA 490
           C GT CFKLSF+II AV+LFG LVS+VLV+RTKKFYKSDIY+KFRE   AA
Sbjct: 520 CIGTSCFKLSFIIIAAVTLFGVLVSMVLVIRTKKFYKSDIYKKFREKALAA 570

[Close]

BLAST of Lsi04G000990 vs. TAIR10
Match: AT2G28120.1 (AT2G28120.1 Major facilitator superfamily protein)

HSP 1 Score: 326.2 bits (835), Expect = 3.4e-89
Identity = 159/249 (63.86%), Postives = 201/249 (80.72%), Query Frame = 1


Query: 240 WKNVFNPPPRGDDWTILQALFSFDMFLLFLATACGVGGTLTAIDNLGQIGQSQNYPKKSI 299
           +  VF+PPPRG+D+TILQAL S DM +LF+AT CG+G +LTA+DNLGQIG+S  YP  ++
Sbjct: 312 FSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESLGYPNHTV 371

Query: 300 STFVSLVSIWNYLGRVMAGFLSEHLLIKYKFPRPLMLTIVLLLSCIAHLLIAFNPAGGLY 359
           S+FVSLVSIWNY GRV +GF+SE+LL KYK PRPLM+T+VLLLSC  HLLIAF   G +Y
Sbjct: 372 SSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIAFPVPGSVY 431

Query: 360 IASVMTGFCYGAQWPLLFAIVSEIFGLKYYATLYNFGSVASPVGLYVLNVNVAGYLYDKE 419
           IAS++ GF +GAQ PLLFAI+SE+FGLKYY+TL+N G +ASP+G Y+LNV V G LYDKE
Sbjct: 432 IASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRVTGMLYDKE 491

Query: 420 AKKQLAMAGRIRKTGEELICHGTVCFKLSFVIITAVSLFGALVSLVLVLRTKKFYKSDIY 479
           A KQL   G  RK  ++L C G+ C+KL F+I+ AV+ FGALVSL L +RT++FYK DIY
Sbjct: 492 ALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRTREFYKGDIY 551

Query: 480 RKFRETEEA 489
           +KFRE+ E+
Sbjct: 552 KKFRESPES 560

[Close]

BLAST of Lsi04G000990 vs. TAIR10
Match: AT1G74780.1 (AT1G74780.1 Nodulin-like / Major Facilitator Superfamily protein)

HSP 1 Score: 191.4 bits (485), Expect = 1.3e-48
Identity = 100/231 (43.29%), Postives = 148/231 (64.07%), Query Frame = 1


Query: 251 DDWTILQALFSFDMFLLFLATACGVGGTLTAIDNLGQIGQSQNYPKKSISTFVSLVSIWN 310
           ++  +LQA+     +LLFLA  CG+G  L+ I+N+ QIG+S  Y    I++ VSL SIWN
Sbjct: 311 ENLNLLQAMKKLSFWLLFLAMICGMGSGLSTINNIRQIGESLRYSSVEINSLVSLWSIWN 370

Query: 311 YLGRVMAGFLSEHLLIKYKFPRPLMLTIVLLLSCIAHLLIAFNPAGGLYIASVMTGFCYG 370
           +LGR  AG+ S+ LL K  +PRPL++   L    I HL+IA    G LY+ SV+ G CYG
Sbjct: 371 FLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSIGHLIIASGFQGNLYVGSVIVGVCYG 430

Query: 371 AQWPLLFAIVSEIFGLKYYATLYNFGSVASPVGLYVLNVNVAGYLYDKEAKKQLAMAGRI 430
           +QW L+  I SE+FG+++  T++N  SVASP+G Y+ +V + GY+YDK A          
Sbjct: 431 SQWSLMPTITSELFGIRHMGTIFNTISVASPIGSYIFSVRLIGYIYDKTA---------- 490

Query: 431 RKTGEELICHGTVCFKLSFVIITAVSLFGALVSLVLVLRTKKFYKSDIYRK 482
             +GE   C+G+ CF+LSF+I+ +V+ FG LV++VL  RTK  Y+  + ++
Sbjct: 491 --SGEGNTCYGSHCFRLSFIIMASVAFFGFLVAIVLFFRTKTLYRQILVKR 529

[Close]

BLAST of Lsi04G000990 vs. TAIR10
Match: AT2G34355.1 (AT2G34355.1 Major facilitator superfamily protein)

HSP 1 Score: 185.7 bits (470), Expect = 7.1e-47
Identity = 95/231 (41.13%), Postives = 147/231 (63.64%), Query Frame = 1


Query: 251 DDWTILQALFSFDMFLLFLATACGVGGTLTAIDNLGQIGQSQNYPKKSISTFVSLVSIWN 310
           +D  IL+A+ + + +LLFLA  CG+G     ++N+ QIG+S  Y    +++ VSL SIWN
Sbjct: 302 EDSNILEAMSTVNFWLLFLAMLCGMGSGFATVNNMRQIGESLRYSSVQLNSLVSLWSIWN 361

Query: 311 YLGRVMAGFLSEHLLIKYKFPRPLMLTIVLLLSCIAHLLIAFNPAGGLYIASVMTGFCYG 370
           +LGR  AG++S+  L K+ +PRP+ + I L +  I H+++A    G LY  SV+ G  YG
Sbjct: 362 FLGRFGAGYVSDTFLHKHSWPRPIFMAITLGVMAIGHIIVASGVQGSLYAGSVLIGMAYG 421

Query: 371 AQWPLLFAIVSEIFGLKYYATLYNFGSVASPVGLYVLNVNVAGYLYDKEAKKQLAMAGRI 430
           +QW L+  I SEIFG+++  T+Y   S+A P+G Y+L+V V GY YDK A +        
Sbjct: 422 SQWSLMPTITSEIFGIRHMGTIYFTISIAGPIGSYILSVKVIGYFYDKVASE-------- 481

Query: 431 RKTGEELICHGTVCFKLSFVIITAVSLFGALVSLVLVLRTKKFYKSDIYRK 482
               ++  C G+ CF+ SF+I+ +V+LFG+LV+ VL  RT KFYK+ + ++
Sbjct: 482 ----DDNSCFGSQCFRTSFMIMASVALFGSLVASVLFFRTHKFYKNLVAKR 520

[Close]

BLAST of Lsi04G000990 vs. TAIR10
Match: AT2G34350.1 (AT2G34350.1 Nodulin-like / Major Facilitator Superfamily protein)

HSP 1 Score: 181.0 bits (458), Expect = 1.7e-45
Identity = 95/231 (41.13%), Postives = 149/231 (64.50%), Query Frame = 1


Query: 251 DDWTILQALFSFDMFLLFLATACGVGGTLTAIDNLGQIGQSQNYPKKSISTFVSLVSIWN 310
           +D  +L+A+ + + +LLF+A  CG+G  L  I+N+ Q+G+S  Y    +++ VSL SIWN
Sbjct: 304 NDMNVLEAICTTNFWLLFVAMICGMGSGLATINNIRQMGESLRYSTVQLNSLVSLWSIWN 363

Query: 311 YLGRVMAGFLSEHLLIKYKFPRPLMLTIVLLLSCIAHLLIAFNPAGGLYIASVMTGFCYG 370
           +LGR  +G++S+  L  + +PRP+ + I L L  I H+++A    G LYI S++ G  YG
Sbjct: 364 FLGRFGSGYISDTYLHSHGWPRPVFMAITLGLMAIGHIVMASGLLGSLYIGSLLVGLAYG 423

Query: 371 AQWPLLFAIVSEIFGLKYYATLYNFGSVASPVGLYVLNVNVAGYLYDKEAKKQLAMAGRI 430
           +QW L+  I SEIFG+ +  T++   S+ASPVG Y  +V V GYLYDK A +        
Sbjct: 424 SQWSLMPTITSEIFGVLHMGTIFYTISIASPVGSYFFSVKVIGYLYDKVASE-------- 483

Query: 431 RKTGEELICHGTVCFKLSFVIITAVSLFGALVSLVLVLRTKKFYKSDIYRK 482
               ++  C+G  CF+ SF+I+ A++L G+LV+LVL+LRTKKFY + + ++
Sbjct: 484 ----DDHSCYGNHCFRTSFLIMAAMALLGSLVALVLLLRTKKFYATLVAKR 522

[Close]

BLAST of Lsi04G000990 vs. NCBI nr
Match: gi|802661142|ref|XP_012080986.1| (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 [Jatropha curcas])

HSP 1 Score: 622.1 bits (1603), Expect = 8.4e-175
Identity = 324/523 (61.95%), Postives = 391/523 (74.76%), Query Frame = 1


Query: 5   NLKSFTLNLIKGSWFMMFASFLIMSMAGIPYMFGLYSSTIKSVLGYDQTTLNFISFFKDV 64
           ++K+ T+ +I G WFM+FASF+IM+ +G  YMFGLYS+ IK+ LGYDQT L  +SFFKD+
Sbjct: 17  DMKNLTVQVINGRWFMVFASFVIMAASGATYMFGLYSNDIKTALGYDQTKLTLLSFFKDL 76

Query: 65  GTTVGVVAGLINEVTPPWSILAMGAVLNFFGFFMIWLSVSEKIP-THVWLMCLYICIGAN 124
           G+++GV +GLINE+TPPW +L+MGAVLNFFG+FMIWL+VS KI   H W MCLYI IGAN
Sbjct: 77  GSSLGVFSGLINELTPPWVVLSMGAVLNFFGYFMIWLAVSGKISGVHFWHMCLYIYIGAN 136

Query: 125 ATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGIDEKSLILLLGW 184
           +  FANTG+LVTCVK +P+ RG VI ILKGY+GLSGAI+TQ+YHAIYG D KSLILL+GW
Sbjct: 137 SQAFANTGSLVTCVKTFPESRGAVIVILKGYVGLSGAIITQIYHAIYGDDSKSLILLIGW 196

Query: 185 LPAAVSLVFMPTVRRMK---------------------------VEHEEDELKGSPPL-- 244
           +PAA+S  F+ TVR MK                           V  EE ++  S  L  
Sbjct: 197 VPAAISFAFLRTVRIMKKRMQFTRSEYGASAAMVLLLLFLPLAIVLIEEYKIWQSKKLVS 256

Query: 245 KNTVPMSLIPKKPKPQPQQPI---------KVKWWKNVFNPPPRGDDWTILQALFSFDMF 304
            N+ P+ +I  +                  KV  WK  F PP RG+D+TILQALFS DM 
Sbjct: 257 NNSSPLKIITTEENTATASTAVLSENVIEEKVSCWKTAFKPPSRGEDYTILQALFSIDML 316

Query: 305 LLFLATACGVGGTLTAIDNLGQIGQSQNYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLL 364
           +LFLAT  GVGGTLTAIDNLGQIG S  YPK+SISTFVSLVSIWNYLGRV+AGF SEH L
Sbjct: 317 VLFLATIFGVGGTLTAIDNLGQIGTSLGYPKRSISTFVSLVSIWNYLGRVVAGFASEHFL 376

Query: 365 IKYKFPRPLMLTIVLLLSCIAHLLIAFNPAGGLYIASVMTGFCYGAQWPLLFAIVSEIFG 424
            KYKFPRPLM T++LL SC+ HLLIA+N  GGLY+AS++ GFC+GAQWPLLFAI+SEIFG
Sbjct: 377 TKYKFPRPLMFTLILLCSCVGHLLIAYNVPGGLYVASIIIGFCFGAQWPLLFAIISEIFG 436

Query: 425 LKYYATLYNFGSVASPVGLYVLNVNVAGYLYDKEAKKQLAMAGRIRKTGEELICHGTVCF 484
           LKYY+TLYNF  +ASP+G +VLNV VAG+LYD EAKKQLA  G  R+ G+EL C GT CF
Sbjct: 437 LKYYSTLYNFAGMASPIGSFVLNVRVAGHLYDIEAKKQLAAKGLTRQHGQELSCIGTECF 496

Query: 485 KLSFVIITAVSLFGALVSLVLVLRTKKFYKSDIYRKFRETEEA 489
           KLSF+IITAV++F   VSL+LVLRT+KFYKSDIY+KFRE  +A
Sbjct: 497 KLSFIIITAVTIFSTFVSLILVLRTRKFYKSDIYKKFREAAQA 539

[Close]

BLAST of Lsi04G000990 vs. NCBI nr
Match: gi|296085964|emb|CBI31405.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 568.5 bits (1464), Expect = 1.1e-158
Identity = 307/519 (59.15%), Postives = 375/519 (72.25%), Query Frame = 1


Query: 5   NLKSFTLNLIKGSWFMMFASFLIMSMAGIPYMFGLYSSTIKSVLGYDQTTLNFISFFKDV 64
           ++KS ++ +I G WF++FASFLIMS AG  YMFGLYS TIKS LGYDQTTLN +SFFKD+
Sbjct: 15  DMKSLSVQVITGRWFVVFASFLIMSAAGATYMFGLYSGTIKSALGYDQTTLNLLSFFKDL 74

Query: 65  GTTVGVVAGLINEVTPPWSILAMGAVLNFFGFFMIWLSVSEKIPT-HVWLMCLYICIGAN 124
           G  VGV++GLINEVTPPW +L+MGA LNFFG+FMIWL+VS+KI    VW MCLYICIGAN
Sbjct: 75  GANVGVLSGLINEVTPPWVVLSMGAALNFFGYFMIWLAVSQKIAKPQVWHMCLYICIGAN 134

Query: 125 ATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGIDEKSLILLLGW 184
           +  FANTG+LVTCVKN+P+ RGVV+GILKGY+GLSGAI+TQLYHA YG D K+LILL+ W
Sbjct: 135 SQAFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYHAFYGNDTKALILLIAW 194

Query: 185 LPAAVS------LVFMPTVRRMK------------------------VEHE----EDELK 244
           LPAA+S      +  M  +R+                          VE E    + E  
Sbjct: 195 LPAAISFAFLRTIRIMKVIRQENELKVFYNFLYISLGLAGFLMIIIIVEKELTFSQSEYG 254

Query: 245 GSPPLKNTV---PMSLIPKKPKP--QPQQPIKVKWWKNVFNPPPRGDDWTILQALFSFDM 304
           GS  L   +   P++++ ++     + +Q   +  + NVF PP RG+D+TILQALFS DM
Sbjct: 255 GSAALVLLLLFLPLAVVIQEEFKLWKIKQEALLSCFSNVFRPPDRGEDYTILQALFSIDM 314

Query: 305 FLLFLATACGVGGTLTAIDNLGQIGQSQNYPKKSISTFVSLVSIWNYLGRVMAGFLSEHL 364
            +LF  T CGVGGTLTAIDNLGQIG S  YP+KS+STF+SLVSIWNYLGRV AGF SE +
Sbjct: 315 LILFFTTICGVGGTLTAIDNLGQIGSSLGYPQKSLSTFISLVSIWNYLGRVTAGFGSEIV 374

Query: 365 LIKYKFPRPLMLTIVLLLSCIAHLLIAFNPAGGLYIASVMTGFCYGAQWPLLFAIVSEIF 424
           L KYKFPRPLMLT++LLLSC+ HLLIAFN   GLY AS++ GFC+GAQWPLLFA++SEIF
Sbjct: 375 LDKYKFPRPLMLTLILLLSCVGHLLIAFNIKNGLYFASIIIGFCFGAQWPLLFAVISEIF 434

Query: 425 GLKYYATLYNFGSVASPVGLYVLNVNVAGYLYDKEAKKQLAMAGRIRKTGEELICHGTVC 484
           GLKYY+TLYNFGSVASP+G Y+LNV                      + GE+L C G  C
Sbjct: 435 GLKYYSTLYNFGSVASPIGSYLLNV----------------------RRGEDLDCTGVEC 494

[Close]

BLAST of Lsi04G000990 vs. NCBI nr
Match: gi|947083832|gb|KRH32553.1| (hypothetical protein GLYMA_10G058800 [Glycine max])

HSP 1 Score: 545.4 bits (1404), Expect = 1.0e-151
Identity = 285/499 (57.11%), Postives = 365/499 (73.15%), Query Frame = 1


Query: 8   SFTLNLIKGSWFMMFASFLIMSMAGIPYMFGLYSSTIKSVLGYDQTTLNFISFFKDVGTT 67
           +F  ++I G WFM+FAS LIM+++G  YMFGLYS+ +K+ LGYDQ+TLN ISFFKD+G  
Sbjct: 5   AFIHHVITGRWFMIFASCLIMAVSGATYMFGLYSNEVKASLGYDQSTLNLISFFKDLGAN 64

Query: 68  VGVVAGLINEVTPPWSILAMGAVLNFFGFFMIWLSVSEKIPT-HVWLMCLYICIGANATT 127
           +G+ +GLINE++PPW ILAMGA +NF G+FMIWLSV+ +I    VW MCLY  IGAN+ +
Sbjct: 65  LGIFSGLINEISPPWVILAMGATMNFIGYFMIWLSVTSRIAKPQVWQMCLYFYIGANSQS 124

Query: 128 FANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYG--------------- 187
           FANTGALV CVK++P+ RG VIG+LKGY+GLSGAI TQ YHA YG               
Sbjct: 125 FANTGALVNCVKSFPRSRGSVIGLLKGYVGLSGAIFTQFYHAFYGFLMVLIVVQNKLSFT 184

Query: 188 ----IDEKSLILLLGWLPAAVSLVFMPTVRRMKVEHEEDELKGSPPLKNTVPMSLIPKKP 247
               I +  ++LLL  LP  + +VF    +  K +++      +    + V +S  P++ 
Sbjct: 185 RVEFIVDGMVVLLLLLLP--LGIVFKEEFKIWKNQNQNF----TDAAASVVELSQ-PEEA 244

Query: 248 KPQPQQPIKVKWWKNVFNPPPRGDDWTILQALFSFDMFLLFLATACGVGGTLTAIDNLGQ 307
               ++       KNVF PP RG+D+TI QALFS DM +LF+AT  GVGGTLTA+DNLGQ
Sbjct: 245 PSHSERKNNNSCLKNVFKPPKRGEDYTIFQALFSIDMLILFIATVFGVGGTLTALDNLGQ 304

Query: 308 IGQSQNYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYKFPRPLMLTIVLLLSCIAH 367
           IG S  YPKKS++TFVSLVSIWNYLGR  +GF+SE+LL KYKFPRPL+LT+V+LLSC+ H
Sbjct: 305 IGNSLGYPKKSLTTFVSLVSIWNYLGRASSGFVSEYLLTKYKFPRPLLLTLVMLLSCVGH 364

Query: 368 LLIAFNPAGGLYIASVMTGFCYGAQWPLLFAIVSEIFGLKYYATLYNFGSVASPVGLYVL 427
           +LIAF     LY +SV+ GFC+GA WPL+FAI+SEIFGLKYY+TLYNFG+VASPVG Y+L
Sbjct: 365 ILIAFGIPNSLYFSSVIIGFCFGAIWPLMFAIISEIFGLKYYSTLYNFGAVASPVGSYIL 424

Query: 428 NVNVAGYLYDKEAKKQLAMAGRIRKTGEELICHGTVCFKLSFVIITAVSLFGALVSLVLV 487
           NV V GYLYDKEA KQL + G IR+ G++L C G  C++++F+IITA +L G +VS +LV
Sbjct: 425 NVRVTGYLYDKEALKQLGVKGLIRQKGKDLTCVGVQCYRMAFLIITASTLVGCVVSFILV 484

[Close]

BLAST of Lsi04G000990 vs. NCBI nr
Match: gi|763806555|gb|KJB73493.1| (hypothetical protein B456_011G234900, partial [Gossypium raimondii])

HSP 1 Score: 531.6 bits (1368), Expect = 1.5e-147
Identity = 272/489 (55.62%), Postives = 363/489 (74.23%), Query Frame = 1


Query: 3   GFNLKSFTLNLIKGSWFMMFASFLIMSMAGIPYMFGLYSSTIKSVLGYDQTTLNFISFFK 62
           G+    F L +I G WFMMFASFLIM+ AG  Y+FG+YS  IKS LGYDQ+TLN +S FK
Sbjct: 16  GYVCCRFFLQVITGRWFMMFASFLIMACAGATYLFGVYSKDIKSTLGYDQSTLNLMSTFK 75

Query: 63  DVGTTVGVVAGLINEVTPPWSILAMGAVLNFFGFFMIWLSVSEKIPT-HVWLMCLYICIG 122
           D+G  VGV++GL+ EVTP W +L +GA++NF G+FMIWL+V++++ T  VW MC YICIG
Sbjct: 76  DLGANVGVLSGLLAEVTPTWFVLLVGALMNFTGYFMIWLAVTKRVATPKVWQMCAYICIG 135

Query: 123 ANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGIDEKSLILLL 182
           AN+  FANTG+LVTCVKN+PQ RG+++G++KG++GLSGAI  Q+Y+AIYG D KSLILL+
Sbjct: 136 ANSQNFANTGSLVTCVKNFPQSRGMMLGLMKGFVGLSGAIFPQIYYAIYGNDSKSLILLI 195

Query: 183 GWLPAAVSLVFMPTVRRMKVEHEEDELK------GSPPLKNTVPMSLIPKKPKPQPQQPI 242
           GW PAA+SLVF+  +R MK+    +E K      G   +   + M+L   + K +     
Sbjct: 196 GWFPAAISLVFVYNIRAMKISTHPNETKVLHEYLGIATVLALLIMALTIAQKKTKEAS-- 255

Query: 243 KVKWWKNVFNPPPRGDDWTILQALFSFDMFLLFLATACGVGGTLTAIDNLGQIGQSQNYP 302
                 ++F PP RG+D++ILQALFS DMFL+FL T CG+G +LTA+DNLGQIG+S  YP
Sbjct: 256 -ASGCSSIFKPPKRGEDFSILQALFSIDMFLVFLGTFCGLGCSLTAVDNLGQIGESLGYP 315

Query: 303 KKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYKFPRPLMLTIVLLLSCIAHLLIAFNPA 362
            +++ST VSL+SIWNY GRV AGF+SE +L+KYK PRPL++++V LL+ +  LLIAF   
Sbjct: 316 YQAVSTVVSLLSIWNYFGRVFAGFVSEIILLKYKIPRPLIMSLVFLLAAVGDLLIAFPWP 375

Query: 363 GGLYIASVMTGFCYGAQWPLLFAIVSEIFGLKYYATLYNFGSVASPVGLYVLNVNVAGYL 422
           G +Y+AS++ GF +GAQ  LLF IVSE+FGLKYYATL+N   ++SP+G +VLNV + G L
Sbjct: 376 GSVYLASLLLGFSFGAQQTLLFTIVSELFGLKYYATLFNCAQLSSPLGSFVLNVRIVGKL 435

Query: 423 YDKEAKKQLAMAGRIRKTGEELICHGTVCFKLSFVIITAVSLFGALVSLVLVLRTKKFYK 482
           YDKEA KQLA  G  R   +EL C G  C++LSF I+ A + FG++V LVLVLRT+K+Y 
Sbjct: 436 YDKEAWKQLAAKGMARSMVKELTCIGEQCYRLSFSILAATNTFGSVVILVLVLRTRKYYG 495

Query: 483 SDIYRKFRE 485
            DIY++FR+
Sbjct: 496 GDIYKRFRD 501

[Close]

BLAST of Lsi04G000990 vs. NCBI nr
Match: gi|659091946|ref|XP_008446818.1| (PREDICTED: uncharacterized protein LOC103489434 [Cucumis melo])

HSP 1 Score: 529.3 bits (1362), Expect = 7.4e-147
Identity = 263/280 (93.93%), Postives = 275/280 (98.21%), Query Frame = 1


Query: 210 GSPPLKNTVPMSLIPKKPKPQPQQPIKVKWWKNVFNPPPRGDDWTILQALFSFDMFLLFL 269
           GSPPLKNT PMSL+PKK KPQ QQPIK +WWKNVFNPPPRGDDWTILQALFSFDMFLLFL
Sbjct: 286 GSPPLKNTTPMSLLPKKQKPQQQQPIKTEWWKNVFNPPPRGDDWTILQALFSFDMFLLFL 345

Query: 270 ATACGVGGTLTAIDNLGQIGQSQNYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYK 329
           ATACGVGGTLTAIDNLGQIGQSQ+YPKKSISTFVSLVSIWNYLGRVMAGFLSEHLL+KYK
Sbjct: 346 ATACGVGGTLTAIDNLGQIGQSQHYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLVKYK 405

Query: 330 FPRPLMLTIVLLLSCIAHLLIAFNPAGGLYIASVMTGFCYGAQWPLLFAIVSEIFGLKYY 389
           FPRPLMLTIVLLLSCIAHLLIAFNP+GGLYIAS++TGFCYGAQWPLLFAIVSEIFGLKYY
Sbjct: 406 FPRPLMLTIVLLLSCIAHLLIAFNPSGGLYIASILTGFCYGAQWPLLFAIVSEIFGLKYY 465

Query: 390 ATLYNFGSVASPVGLYVLNVNVAGYLYDKEAKKQLAMAGRIRKTGEELICHGTVCFKLSF 449
           ATLYNFGSVASPVGLY+LNVNVAGYLYDKEAKKQLAMAGRIRKTGEEL+C+GTVCFKLSF
Sbjct: 466 ATLYNFGSVASPVGLYLLNVNVAGYLYDKEAKKQLAMAGRIRKTGEELVCNGTVCFKLSF 525

Query: 450 VIITAVSLFGALVSLVLVLRTKKFYKSDIYRKFRETEEAA 490
           VIITAVSLFGALVSLVLVLRTKKFYKSDIY+KF+E EEAA
Sbjct: 526 VIITAVSLFGALVSLVLVLRTKKFYKSDIYKKFKEAEEAA 565

The following BLAST results are available for this feature:

BLAST of Lsi04G000990 vs. Swiss-Prot


Match Name	E-value	Identity	Description
NFD4_ARATH	4.7e-08	24.23	Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana GN=NFD4 PE=3 SV=1	[more]

BLAST of Lsi04G000990 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0R0HXG0_SOYBN	7.0e-152	57.11	Uncharacterized protein OS=Glycine max GN=GLYMA_10G058800 PE=4 SV=1	[more]
A0A0D2TCP2_GOSRA	1.0e-147	55.62	Uncharacterized protein (Fragment) OS=Gossypium raimondii GN=B456_011G234900 PE=...	[more]
A0A0A0KWQ1_CUCSA	4.8e-145	92.17	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G622530 PE=4 SV=1	[more]
A0A061EU62_THECC	8.2e-145	54.13	Major facilitator superfamily protein, putative OS=Theobroma cacao GN=TCM_022284...	[more]
B9GP32_POPTR	1.4e-144	54.69	Uncharacterized protein (Fragment) OS=Populus trichocarpa GN=POPTR_0002s13500g P...	[more]

BLAST of Lsi04G000990 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G39210.1	7.5e-105	68.04	Major facilitator superfamily protein	[more]
AT2G28120.1	3.4e-89	63.86	Major facilitator superfamily protein	[more]
AT1G74780.1	1.3e-48	43.29	Nodulin-like / Major Facilitator Superfamily protein	[more]
AT2G34355.1	7.1e-47	41.13	Major facilitator superfamily protein	[more]
AT2G34350.1	1.7e-45	41.13	Nodulin-like / Major Facilitator Superfamily protein	[more]

BLAST of Lsi04G000990 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|802661142\|ref\|XP_012080986.1\|	8.4e-175	61.95	PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 [Jatropha curcas]	[more]
gi\|296085964\|emb\|CBI31405.3\|	1.1e-158	59.15	unnamed protein product [Vitis vinifera]	[more]
gi\|947083832\|gb\|KRH32553.1\|	1.0e-151	57.11	hypothetical protein GLYMA_10G058800 [Glycine max]	[more]
gi\|763806555\|gb\|KJB73493.1\|	1.5e-147	55.62	hypothetical protein B456_011G234900, partial [Gossypium raimondii]	[more]
gi\|659091946\|ref\|XP_008446818.1\|	7.4e-147	93.93	PREDICTED: uncharacterized protein LOC103489434 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0055085	transmembrane transport

Vocabulary: **Cellular Component**
Term	Definition
GO:0016021	integral component of membrane

Vocabulary: **INTERPRO**
Term	Definition
IPR020846	MFS_dom
IPR011701	MFS
IPR010658	Nodulin-like

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
biological_process	GO:0002239	response to oomycetes
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009506	plasmodesma
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G000990.1	Lsi04G000990.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010658	Nodulin-like	PFAM	PF06813	Nodulin-like	coord: 18..202 score: 2.6
IPR011701	Major facilitator superfamily	PFAM	PF07690	MFS_1	coord: 268..471 score: 2.3
IPR020846	Major facilitator superfamily domain	unknown	SSF103473	MFS general substrate transporter	coord: 18..206 score: 3.4E-27coord: 245..473 score: 3.4
None	No IPR available	GENE3D	G3DSA:1.20.1250.20		coord: 261..472 score: 1.5E-15coord: 21..207 score: 7.
None	No IPR available	PANTHER	PTHR21576	UNCHARACTERIZED NODULIN-LIKE PROTEIN	coord: 5..490 score:
None	No IPR available	PANTHER	PTHR21576:SF40	SUBFAMILY NOT NAMED	coord: 5..490 score:

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi04G000990	CsGy5G026220	Cucumber (Gy14) v2	cgyblsiB337
Lsi04G000990	CsaV3_5G035920	Cucumber (Chinese Long) v3	cuclsiB393

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi04G000990	Silver-seed gourd	carlsiB240
Lsi04G000990	Silver-seed gourd	carlsiB437
Lsi04G000990	Silver-seed gourd	carlsiB627
Lsi04G000990	Silver-seed gourd	carlsiB767
Lsi04G000990	Watermelon (97103) v2	lsiwmbB284
Lsi04G000990	Watermelon (97103) v2	lsiwmbB300
Lsi04G000990	Wax gourd	lsiwgoB365
Lsi04G000990	Wax gourd	lsiwgoB387
Lsi04G000990	Bottle gourd (USVL1VR-Ls)	lsilsiB152
Lsi04G000990	Cucumber (Gy14) v1	cgylsiB497
Lsi04G000990	Cucurbita maxima (Rimu)	cmalsiB279
Lsi04G000990	Cucurbita maxima (Rimu)	cmalsiB379
Lsi04G000990	Cucurbita maxima (Rimu)	cmalsiB669
Lsi04G000990	Cucurbita maxima (Rimu)	cmalsiB684
Lsi04G000990	Cucurbita moschata (Rifu)	cmolsiB268
Lsi04G000990	Cucurbita moschata (Rifu)	cmolsiB364
Lsi04G000990	Cucurbita moschata (Rifu)	cmolsiB660
Lsi04G000990	Cucurbita moschata (Rifu)	cmolsiB676
Lsi04G000990	Cucurbita moschata (Rifu)	cmolsiB750
Lsi04G000990	Cucurbita pepo (Zucchini)	cpelsiB032
Lsi04G000990	Cucurbita pepo (Zucchini)	cpelsiB151
Lsi04G000990	Cucurbita pepo (Zucchini)	cpelsiB365
Lsi04G000990	Cucurbita pepo (Zucchini)	cpelsiB352
Lsi04G000990	Wild cucumber (PI 183967)	cpilsiB372
Lsi04G000990	Cucumber (Chinese Long) v2	culsiB368
Lsi04G000990	Melon (DHL92) v3.5.1	lsimeB281
Lsi04G000990	Melon (DHL92) v3.5.1	lsimeB304
Lsi04G000990	Watermelon (Charleston Gray)	lsiwcgB283
Lsi04G000990	Watermelon (Charleston Gray)	lsiwcgB300
Lsi04G000990	Watermelon (97103) v1	lsiwmB289
Lsi04G000990	Watermelon (97103) v1	lsiwmB312
Lsi04G000990	Cucumber (Gy14) v2	cgyblsiB421
Lsi04G000990	Melon (DHL92) v3.6.1	lsimedB297
Lsi04G000990	Melon (DHL92) v3.6.1	lsimedB319