Lsi03G014590 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi03G014590
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Auxin efflux carrier component
Location	chr03 : 25478239 .. 25480900 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTCCAATGAACAGTGGCTTAAAACCCGAGCCTTTATTGATTAATAAAGCAATCAAGAAAAGCAGAGGAAAGTGCTTTATTAACAAGGAATTTCTATAGACAATACGAAAAATTTTCAATCCTTCTTCCGCTGGTCACCATGATTTCGTTGTCTGATGTCTATCATGTTGTGGCAGCCACAGTCCCTCTGTACGTAGCCATGATGTTAGCTTACATCTCTGTAAAATGGTGGAAGTTCTTCACAGCGGAGCAATGTTCTGGAATAAACAAGTTTGTGGCAAAATTTTCAATCCCACTGTTATCCTTTCAAGTCATTTCAAACAACAACCTCTACAAAATGAACCTGCAACTGATATTGGCCGACTTCCTTCAGAAAATATTAGCTATTGTTCTGCTGGGTGCAATCACTAGAATCACCTCTCGGGGAGGCTTAAATTGGATTATCACTGGTCTCTCTCTGTCAACCATGCCAAATACTCTAATTCTTGGACTACCAGTATTGAAGGCCATGTACGGCAGTGAAGCAGATGTGCTTCTTGCCCAGATAGTTGTCTTACAGAGCATTCTTTGGTACAACATATTACTGTTTCTCTTTGAGTTCACTACCACCAAAGCAGCTTCTTTAGCTCCAGCATCAGAAGCCACAGGTAGGCATTGGAATTTGCTTTTTAAAAATAGTAAAAAAGAGAATCAAATCATCTCAACTTTATAAGTCATTATGTGTATAAGCTTAGGACTTCCCTAAACAAAAATGGTCATATTCATGCCAATGCCATGATCTTCAGTAACCATCCCAGTTTAAGCCTTCATGTATAGCATTAGGACTTCCCTCAACAGAAATGGCTGTATTCATGCTATCAACCTCAATAACATTCCGTCATGATATCATCCATGTACGACCAAGAATGGAAACTTGCTAAATTTTTCTCTTGATTACTTTACCTCATTTCCTAAATTTTTATTCTCTTCCTACATCCGTAGGTCATGAGTGCTATATCTAGGCTACTGTATTTCGCAGAAAGAATACCCAACATAGTCTCACATGAATTCACGTAACAAAAATGTGTAAGCTAACTTTGTCCACTTCATTCACTCTAGTTATGCTGTTTATCTTTTTGGAAAGCAGAAATTGATATAACTCAAGATGGAAGACCAAAAGAAGAAATTGAGGAGGCAAGAGACAGAAATGGAAGGAGTCTCAAAACCAGGTCCATTCTCTTAACAGTTGCTAGGAAGCTCATCATCAACCCCAATACTCATGCAACTATATTGGGTCTCATTTGGGCAAGCATACGATTCAGGTAAATTAGAGATAATAGCTTTCACTTCAAAAGAAGGTATAGATGTATTAGGATGAAACTAACATTATCTCAGGTGGGGAGTAAAACTGCCAGAAGTTATTGACAGATCAATATCAATTCTATCAACTGGGGGACTTGGTATGGCTATGTTCAGCTTAGGTATGAAACGAAAACATTACTAGAAGTTCCATCAAGTAAATGACGAGAAACATAACTTCTAGTAGTCTGACTCTTACTTATTAGGTCTTTTCATGGGTTCACGGACTAGCATTATAGCATGTGGTACAAAGATGACACTGGTGGCCATGGGAATGAAATTCTTAATGGGGCCTGCACTTATGGCAGCATGCTCACTTGCTCTAGGACTTGGGGGCAGATTGCTCAGAGTGGCAATTGTGCAGGTTTGTTGTAATTTGTATTACTAAGCCCACATCCAGGATTGACAATTCCATTTGACGTGTTTGTTTCTTCTTTCTTCTTCTTCATTGGTACAGGCAGCACTTCCTCAAGGAATTGTTCCATTTGTCTTTTCCAAAGAGTATAATATACATCCAGATGTTTTGAGCACAGGGTAACTATTGTAATTTTCATCTCCATAGCAATTTTCAAACCAGACATTGATCATCTCTTTTATCTAAATAGGCCGAGACAGTAAAACTTTGCTTCATATTATTTTCCAATATTATTCAGCCAAAAGATCATAAAAAGTTTAGGTGGCAACATTTGAGCAGTTTAAGGAGAAGCAAATTATATGGCTAAACTACATACTGAAATGGGGTTTTCTTTTGGTCACAGGGTACTTTTGGGCTTGCTCATTGCACTGCCAGTAGCACTGGCCTACTACTACTTGCTGTCATTATAAAGCATCAATGCAGCCATGCCACCACAGGATACATGGTGCCGCCGAAACAAGAAATTTAGGACATTGACGCTAAACATCAGGAAAGACCACATGCAGAGATGAAGTTTTTGAGTTAAAATTTTTTTACCATTTTTTTCCGAAATGGTACACTGCTGATGATTTTAAGAAACATGTAAATGTGTGTGCTAAAGATATCGAGGAAGGATGATCATATGCAATAAACAAAATAGAAGGAAAAATGCAATACCTTAAATAGGAAGCACACTGAACAGACCTTCGCTACTAATCTATCACCCAGAACTATCTGCATCCTCGTGTTTGTATAGTCCAACTATCCATTGTTTGACTCTGTAATTTTTTTCAAATATCAGCCGGGCTATGAAGATTCTCTCAAAAACAATCGGAAATTGCATGAAAGTTCATAGAAGAAAGATTATTTTCTCAATCAATTATTTTTCCTCGAGTTCTTCGTATTTATTATTCAAACATTAGATCAAAC

mRNA sequence

CTTCCAATGAACAGTGGCTTAAAACCCGAGCCTTTATTGATTAATAAAGCAATCAAGAAAAGCAGAGGAAAGTGCTTTATTAACAAGGAATTTCTATAGACAATACGAAAAATTTTCAATCCTTCTTCCGCTGGTCACCATGATTTCGTTGTCTGATGTCTATCATGTTGTGGCAGCCACAGTCCCTCTGTACGTAGCCATGATGTTAGCTTACATCTCTGTAAAATGGTGGAAGTTCTTCACAGCGGAGCAATGTTCTGGAATAAACAAGTTTGTGGCAAAATTTTCAATCCCACTGTTATCCTTTCAAGTCATTTCAAACAACAACCTCTACAAAATGAACCTGCAACTGATATTGGCCGACTTCCTTCAGAAAATATTAGCTATTGTTCTGCTGGGTGCAATCACTAGAATCACCTCTCGGGGAGGCTTAAATTGGATTATCACTGGTCTCTCTCTGTCAACCATGCCAAATACTCTAATTCTTGGACTACCAGTATTGAAGGCCATGTACGGCAGTGAAGCAGATGTGCTTCTTGCCCAGATAGTTGTCTTACAGAGCATTCTTTGGTACAACATATTACTGTTTCTCTTTGAGTTCACTACCACCAAAGCAGCTTCTTTAGCTCCAGCATCAGAAGCCACAGAAATTGATATAACTCAAGATGGAAGACCAAAAGAAGAAATTGAGGAGGCAAGAGACAGAAATGGAAGGAGTCTCAAAACCAGGTCCATTCTCTTAACAGTTGCTAGGAAGCTCATCATCAACCCCAATACTCATGCAACTATATTGGGTCTCATTTGGGCAAGCATACGATTCAGGTGGGGAGTAAAACTGCCAGAAGTTATTGACAGATCAATATCAATTCTATCAACTGGGGGACTTGGTATGGCTATGTTCAGCTTAGGTCTTTTCATGGGTTCACGGACTAGCATTATAGCATGTGGTACAAAGATGACACTGGTGGCCATGGGAATGAAATTCTTAATGGGGCCTGCACTTATGGCAGCATGCTCACTTGCTCTAGGACTTGGGGGCAGATTGCTCAGAGTGGCAATTGTGCAGGGTACTTTTGGGCTTGCTCATTGCACTGCCAGTAGCACTGGCCTACTACTACTTGCTGTCATTATAAAGCATCAATGCAGCCATGCCACCACAGGATACATGGTGCCGCCGAAACAAGAAATTTAGGACATTGACGCTAAACATCAGGAAAGACCACATGCAGAGATGAAGTTTTTGAGTTAAAATTTTTTTACCATTTTTTTCCGAAATGGTACACTGCTGATGATTTTAAGAAACATGTAAATGTGTGTGCTAAAGATATCGAGGAAGGATGATCATATGCAATAAACAAAATAGAAGGAAAAATGCAATACCTTAAATAGGAAGCACACTGAACAGACCTTCGCTACTAATCTATCACCCAGAACTATCTGCATCCTCGTGTTTGTATAGTCCAACTATCCATTGTTTGACTCTGTAATTTTTTTCAAATATCAGCCGGGCTATGAAGATTCTCTCAAAAACAATCGGAAATTGCATGAAAGTTCATAGAAGAAAGATTATTTTCTCAATCAATTATTTTTCCTCGAGTTCTTCGTATTTATTATTCAAACATTAGATCAAAC

Coding sequence (CDS)

ATGATTTCGTTGTCTGATGTCTATCATGTTGTGGCAGCCACAGTCCCTCTGTACGTAGCCATGATGTTAGCTTACATCTCTGTAAAATGGTGGAAGTTCTTCACAGCGGAGCAATGTTCTGGAATAAACAAGTTTGTGGCAAAATTTTCAATCCCACTGTTATCCTTTCAAGTCATTTCAAACAACAACCTCTACAAAATGAACCTGCAACTGATATTGGCCGACTTCCTTCAGAAAATATTAGCTATTGTTCTGCTGGGTGCAATCACTAGAATCACCTCTCGGGGAGGCTTAAATTGGATTATCACTGGTCTCTCTCTGTCAACCATGCCAAATACTCTAATTCTTGGACTACCAGTATTGAAGGCCATGTACGGCAGTGAAGCAGATGTGCTTCTTGCCCAGATAGTTGTCTTACAGAGCATTCTTTGGTACAACATATTACTGTTTCTCTTTGAGTTCACTACCACCAAAGCAGCTTCTTTAGCTCCAGCATCAGAAGCCACAGAAATTGATATAACTCAAGATGGAAGACCAAAAGAAGAAATTGAGGAGGCAAGAGACAGAAATGGAAGGAGTCTCAAAACCAGGTCCATTCTCTTAACAGTTGCTAGGAAGCTCATCATCAACCCCAATACTCATGCAACTATATTGGGTCTCATTTGGGCAAGCATACGATTCAGGTGGGGAGTAAAACTGCCAGAAGTTATTGACAGATCAATATCAATTCTATCAACTGGGGGACTTGGTATGGCTATGTTCAGCTTAGGTCTTTTCATGGGTTCACGGACTAGCATTATAGCATGTGGTACAAAGATGACACTGGTGGCCATGGGAATGAAATTCTTAATGGGGCCTGCACTTATGGCAGCATGCTCACTTGCTCTAGGACTTGGGGGCAGATTGCTCAGAGTGGCAATTGTGCAGGGTACTTTTGGGCTTGCTCATTGCACTGCCAGTAGCACTGGCCTACTACTACTTGCTGTCATTATAAAGCATCAATGCAGCCATGCCACCACAGGATACATGGTGCCGCCGAAACAAGAAATTTAG

Protein sequence

MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVISNNNLYKMNLQLILADFLQKILAIVLLGAITRITSRGGLNWIITGLSLSTMPNTLILGLPVLKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIDITQDGRPKEEIEEARDRNGRSLKTRSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRSISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLGGRLLRVAIVQGTFGLAHCTASSTGLLLLAVIIKHQCSHATTGYMVPPKQEI

Homology

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BLAST of Lsi03G014590 vs. Swiss-Prot
Match: PIN8_ARATH (Auxin efflux carrier component 8 OS=Arabidopsis thaliana GN=PIN8 PE=2 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 2.6e-85
Identity = 179/320 (55.94%), Postives = 233/320 (72.81%), Query Frame = 1


Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MIS  D+YHVV+ATVPLYV+M L ++S +  K F+ EQC+GINKFVAKFSIPLLSFQ+IS
Sbjct: 1   MISWLDIYHVVSATVPLYVSMTLGFLSARHLKLFSPEQCAGINKFVAKFSIPLLSFQIIS 60

Query: 61  NNNLYKMNLQLILADFLQKILAIVLLGAITRIT----SRGG-LNWIITGLSLSTMPNTLI 120
            NN +KM+ +LIL+D LQK L +V+L  + R       RGG L W+ITGLS+S +PNTLI
Sbjct: 61  ENNPFKMSPKLILSDILQKFLVVVVLAMVLRFWHPTGGRGGKLGWVITGLSISVLPNTLI 120

Query: 121 LGLPVLKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPAS------EAT 180
           LG+P+L A+YG EA  +L QIVVLQS++WY ILLFLFE    +A   + AS      +  
Sbjct: 121 LGMPILSAIYGDEAASILEQIVVLQSLIWYTILLFLFELNAARALPSSGASLEHTGNDQE 180

Query: 181 EIDITQDGRPKEEIEEARDRNGRSLKTRSILLTVARKLIINPNTHATILGLIWASIRFRW 240
           E +I  + + +E+ EE      RS+ T  ILL   RKLIINPNT+AT++G+IWA++ FR 
Sbjct: 181 EANIEDEPKEEEDEEEVAIVRTRSVGTMKILLKAWRKLIINPNTYATLIGIIWATLHFRL 240

Query: 241 GVKLPEVIDRSISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALM 300
           G  LPE+ID+SI +LS GGLGMAMFSLGLFM S++SIIACGTKM ++ M +KF++GPALM
Sbjct: 241 GWNLPEMIDKSIHLLSDGGLGMAMFSLGLFMASQSSIIACGTKMAIITMLLKFVLGPALM 300

Query: 301 AACSLALGLGGRLLRVAIVQ 310
            A +  + L   L +VAI+Q
Sbjct: 301 IASAYCIRLKSTLFKVAILQ 320

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BLAST of Lsi03G014590 vs. Swiss-Prot
Match: PIN6_ORYSJ (Probable auxin efflux carrier component 6 OS=Oryza sativa subsp. japonica GN=PIN6 PE=2 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 1.2e-55
Identity = 126/316 (39.87%), Postives = 190/316 (60.13%), Query Frame = 1


Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MI   DVY VVAATVPLY A+ L Y SV+WW+ FT EQC  +N+ VA F++P  +F+   
Sbjct: 1   MIGWGDVYKVVAATVPLYFALFLGYGSVRWWRIFTREQCDAVNRLVAFFALPFFTFEFTL 60

Query: 61  NNNLYKMNLQLILADFLQKILAIVLLGAITRITSRGG--LNWIITGLSLSTMPNTLILGL 120
           + + +++N + + AD + K + + ++GA  R  S+GG  ++W IT  SLST+ N+L++G+
Sbjct: 61  HTDPFQVNYRAVAADVISKAVIVAVIGAWARFMSKGGCAVSWSITSFSLSTLTNSLVVGV 120

Query: 121 PVLKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEAT-----EIDI 180
           P+ +AMYG  A  L+ Q+ V Q+I+W  +LLF+ E            +EA      +++ 
Sbjct: 121 PMARAMYGEWAQQLVVQLSVFQAIVWLTLLLFVLEVRKAAIGMYVDGAEAAAAAGKDVEA 180

Query: 181 TQDGRPKEEIEEARDRNGRSLKTRSILLTVARKLIINPNTHATILGLIWASIRFRWGVKL 240
                    +  A      SL   +++  VA KL  NPNT+A+ +G+ WA +  R  + L
Sbjct: 181 AGAAAAAGTVVVAAAAGKPSLW--ALVKVVAHKLARNPNTYASFVGITWACLANRLHIAL 240

Query: 241 PEVIDRSISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACS 300
           P   + S+ I+S  G GMAMFS+GLFM  +  IIACGT    + + +KF +GPA MA  S
Sbjct: 241 PSAFEGSVLIMSKSGTGMAMFSMGLFMAQQEKIIACGTSFAALGLVLKFALGPAAMAIGS 300

Query: 301 LALGLGGRLLRVAIVQ 310
           +A+GL G +LRVAI+Q
Sbjct: 301 IAVGLRGDVLRVAIIQ 314

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BLAST of Lsi03G014590 vs. Swiss-Prot
Match: PIN5_ARATH (Auxin efflux carrier component 5 OS=Arabidopsis thaliana GN=PIN5 PE=2 SV=2)

HSP 1 Score: 214.5 bits (545), Expect = 1.8e-54
Identity = 123/309 (39.81%), Postives = 188/309 (60.84%), Query Frame = 1


Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MI+  DVY V+ A VPLYVA++L Y SVKWW  FT +QC  IN+ V  F++PL + +  +
Sbjct: 1   MINCGDVYKVIEAMVPLYVALILGYGSVKWWHIFTRDQCDAINRLVCYFTLPLFTIEFTA 60

Query: 61  NNNLYKMNLQLILADFLQKILAIVLLGAITRITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           + + + MN + I AD L K++ + +L    + +++G   W IT  SL T+ N+L++G+P+
Sbjct: 61  HVDPFNMNYRFIAADVLSKVIIVTVLALWAKYSNKGSYCWSITSFSLCTLTNSLVVGVPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIDITQDGRPK 180
            KAMYG +A  L+ Q  V Q+I+W  +LLF+ EF   KA   +      ++D       K
Sbjct: 121 AKAMYGQQAVDLVVQSSVFQAIVWLTLLLFVLEF--RKAGFSSNNISDVQVDNINIESGK 180

Query: 181 EEIEEARDRNGRSLKTRSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRS 240
            E     +++        ++  V  KL  NPN ++ ILG+ WA I  RW ++LP +++ S
Sbjct: 181 RETVVVGEKS-----FLEVMSLVWLKLATNPNCYSCILGIAWAFISNRWHLELPGILEGS 240

Query: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLGG 300
           I I+S  G G AMF++G+FM  +  +I CGT +T++ M +KF+ GPA MA  S+ LGL G
Sbjct: 241 ILIMSKAGTGTAMFNMGIFMALQEKLIVCGTSLTVMGMVLKFIAGPAAMAIGSIVLGLHG 300

Query: 301 RLLRVAIVQ 310
            +LRVAI+Q
Sbjct: 301 DVLRVAIIQ 302

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BLAST of Lsi03G014590 vs. Swiss-Prot
Match: PIN4_ORYSJ (Probable auxin efflux carrier component 4 OS=Oryza sativa subsp. japonica GN=PIN4 PE=2 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.3e-52
Identity = 125/325 (38.46%), Postives = 186/325 (57.23%), Query Frame = 1


Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MI   DVY VV A  PLY A+ L Y SV+WW+FFTAEQC+ IN  V  FS+P  +F  + 
Sbjct: 1   MIGWGDVYKVVGAMAPLYFALGLGYGSVRWWRFFTAEQCAAINTMVVYFSMPFFTFDFVV 60

Query: 61  NNNLYKMNLQLILADFLQKILAIVLLGAITRI------TSRGGLNWIITGLSLSTMPNTL 120
             + + MN ++I AD + K +AI  + A  R          G  +W ITG SL+ + NTL
Sbjct: 61  RTDPFAMNYRVIAADAVSKAIAIAAMAAWARTRCGCAAAKAGAQSWSITGFSLAALNNTL 120

Query: 121 ILGLPVLKAMYGSEADVLLAQIVVLQSILWYNILLFLFE----FTTTKAASLAPASEAT- 180
           ++G+P+L AMYG  A  L+ QI V+QS++W+ +LL  FE    +       + PA  ++ 
Sbjct: 121 VVGVPLLDAMYGRWAQDLVVQIAVVQSMVWFPLLLMAFELRKAWVVGGGGGVGPAVMSSS 180

Query: 181 -----EIDITQDGRPKEEIEEARDRNGRSLKTRSILLTVARKLIINPNTHATILGLIWAS 240
                + D+  +G     +       G  L   +   TV  KL  NPN +A++LG++WA 
Sbjct: 181 SPPEKQSDVEMNGAV---VAAPGGGGGVRLPFWATARTVGLKLARNPNVYASVLGVVWAC 240

Query: 241 IRFRWGVKLPEVIDRSISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLM 300
           I +RW + LP ++  S+ ++S  G GM+MFS+GLFMG +  +IACG  +T + M ++F+ 
Sbjct: 241 IAYRWHLSLPGIVTGSLQVMSRTGTGMSMFSMGLFMGQQERVIACGAGLTALGMALRFVA 300

Query: 301 GPALMAACSLALGLGGRLLRVAIVQ 310
           GP      + ALGL G +L +AI+Q
Sbjct: 301 GPLATLVGAAALGLRGDVLHLAIIQ 322

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BLAST of Lsi03G014590 vs. Swiss-Prot
Match: PIN1_ORYSJ (Auxin efflux carrier component 1 OS=Oryza sativa subsp. japonica GN=PIN1 PE=2 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.7e-44
Identity = 90/154 (58.44%), Postives = 119/154 (77.27%), Query Frame = 1


Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MI+ +D YHV+ A VPLYVAM+LAY SVKWW+ FT +QCSGIN+FVA F++PLLSF  IS
Sbjct: 1   MITAADFYHVMTAMVPLYVAMILAYGSVKWWRIFTPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  NNNLYKMNLQLILADFLQKILAIVLLGAITRITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            NN Y MNL+ I AD LQK++ + +L A + ++ RG L W IT  SLST+PNTL++G+P+
Sbjct: 61  TNNPYTMNLRFIAADTLQKLMVLAMLTAWSHLSRRGSLEWTITLFSLSTLPNTLVMGIPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEF 155
           LK MYG  +  L+ QIVVLQ I+WY ++LF+FE+
Sbjct: 121 LKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEY 154

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BLAST of Lsi03G014590 vs. TrEMBL
Match: A0A0A0LFB7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G827360 PE=4 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 4.1e-154
Identity = 296/309 (95.79%), Postives = 305/309 (98.71%), Query Frame = 1


Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  NNNLYKMNLQLILADFLQKILAIVLLGAITRITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            NNLYKMN QLILADFLQKILAI+LLGA+T+ITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIDITQDGRPK 180
           LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEI+IT +GRPK
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITHEGRPK 180

Query: 181 EEIEEARDRNGRSLKTRSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRS 240
           EEIEEARDRNGRSL+T+SILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRS
Sbjct: 181 EEIEEARDRNGRSLRTKSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRS 240

Query: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLGG 300
           ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFL+GPALMAACSLALGL G
Sbjct: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLVGPALMAACSLALGLRG 300

Query: 301 RLLRVAIVQ 310
           +LLRVAIVQ
Sbjct: 301 KLLRVAIVQ 309

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BLAST of Lsi03G014590 vs. TrEMBL
Match: W9QE17_9ROSA (Putative auxin efflux carrier component 5 OS=Morus notabilis GN=L484_001515 PE=4 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 4.5e-113
Identity = 220/313 (70.29%), Postives = 273/313 (87.22%), Query Frame = 1


Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MISL DVYHVV ATVPLYVAM+LAY+SV+WWK FT +QCSGINKFVAKFSIPLLSFQ++S
Sbjct: 1   MISLRDVYHVVTATVPLYVAMILAYVSVRWWKIFTPDQCSGINKFVAKFSIPLLSFQILS 60

Query: 61  NNNLYKMNLQLILADFLQKILAIVLLGAITRITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           +NN YKMNL+LIL+DFLQK+LA V L  +TR TS+GGL+++ITGLS+S +PNTLILG+P+
Sbjct: 61  DNNPYKMNLRLILSDFLQKLLAFVALTVMTRFTSKGGLDFVITGLSVSILPNTLILGIPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTK-AASLAPASEATE-IDITQDGR 180
           LKAMYG +A VLL+QIVVLQS++WYN+LLFL+EF   K AAS++P+SEATE ++  Q+ +
Sbjct: 121 LKAMYGEQAAVLLSQIVVLQSLIWYNLLLFLYEFNAAKSAASVSPSSEATEDMEAPQESQ 180

Query: 181 PKEEIEEARDRNG--RSLKTRSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEV 240
           PKEE+EEAR+R+   R +KT  ILLTV RKLI NPNTHATI+GL+WA I+FRW V+LP +
Sbjct: 181 PKEEVEEARNRSSSPRKIKTMLILLTVGRKLIGNPNTHATIIGLVWACIKFRWKVELPAI 240

Query: 241 IDRSISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLAL 300
           +D+SI++LS+GGLGMAMFSLGLFM SR+SIIACGT+M ++AMG+KF+ GP LMAA SLA+
Sbjct: 241 VDQSITVLSSGGLGMAMFSLGLFMASRSSIIACGTRMGVLAMGLKFIGGPFLMAAASLAI 300

Query: 301 GLGGRLLRVAIVQ 310
           GL G  LRVAIVQ
Sbjct: 301 GLRGTQLRVAIVQ 313

[Close]

BLAST of Lsi03G014590 vs. TrEMBL
Match: M5WVA2_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021573mg PE=4 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 5.0e-112
Identity = 223/312 (71.47%), Postives = 265/312 (84.94%), Query Frame = 1


Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MISL+DVYHVVAATVPLYVAM+LAY+SVKWWK FT +QCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLADVYHVVAATVPLYVAMILAYVSVKWWKLFTPDQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  NNNLYKMNLQLILADFLQKILAIVLLGAITRITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            NN YKMNL+LIL+D LQK++A + L AIT+ +S GGLN IITGLSLSTMPNTLILG+P+
Sbjct: 61  GNNPYKMNLKLILSDCLQKLIAFLALTAITKFSSHGGLNSIITGLSLSTMPNTLILGIPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEAT--EIDITQDGR 180
           LKAMYG EA +L+ QIVVLQS++WYN+LLFLFEF   KAAS+ P+SE+T  E++  Q+ +
Sbjct: 121 LKAMYGDEAAMLVTQIVVLQSLIWYNMLLFLFEFRAAKAASVTPSSESTAEEMEAPQEAQ 180

Query: 181 PKEEIE-EARDRNGRSLKTRSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVI 240
            KEE E E R R  + +K  SILLTV RKLI NPNTHAT+LGLIWASI ++WGVKLP+++
Sbjct: 181 SKEEAEDETRSRTPKKIK--SILLTVGRKLIANPNTHATLLGLIWASIHYKWGVKLPDIV 240

Query: 241 DRSISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALG 300
            +SI+ILS GGLGMAMFSLGLFM SR SIIACGT+  ++AMG+KF+ GPALMA  S A+G
Sbjct: 241 TQSIAILSNGGLGMAMFSLGLFMASRGSIIACGTRTGMLAMGLKFIAGPALMAVSSFAVG 300

Query: 301 LGGRLLRVAIVQ 310
           L GR+LRVAIVQ
Sbjct: 301 LRGRILRVAIVQ 310

[Close]

BLAST of Lsi03G014590 vs. TrEMBL
Match: A0A067FYK2_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g018360mg PE=4 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 2.8e-110
Identity = 216/310 (69.68%), Postives = 257/310 (82.90%), Query Frame = 1


Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MIS+ D YHVVAA VPLYVAM+LAYISVKWWK FT +QC+GINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISVVDAYHVVAACVPLYVAMLLAYISVKWWKLFTPDQCAGINKFVAKFSIPLLSFQVIS 60

Query: 61  NNNLYKMNLQLILADFLQKILAIVLLGAITRITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            NN YKMNL+LI ADFLQK+LA ++L A+T+I+SR GL WIITGLSLS++PN+LILG+P+
Sbjct: 61  KNNPYKMNLKLIYADFLQKLLAFLVLVALTKISSRSGLRWIITGLSLSSLPNSLILGIPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEA-TEIDITQDGRP 180
           L+AMY  EA +LLAQIVVLQS++WYN+LLFLFE    K+AS+ P  E   E D +Q+ + 
Sbjct: 121 LRAMYSEEAALLLAQIVVLQSLIWYNLLLFLFELNAAKSASVTPNPETPEEHDASQESQA 180

Query: 181 KEEIEEARDRNGRSLKTRSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDR 240
           KE  EEA++R    ++T  ILLTV RKLIINPNTHAT+LGLIWASI FRWGVKLP +ID+
Sbjct: 181 KEGEEEAQNRTATRVRTTHILLTVGRKLIINPNTHATLLGLIWASIEFRWGVKLPAIIDQ 240

Query: 241 SISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLG 300
           S+ ILS GGLGMAMFSLGLFM SR SIIACGT+M  VAM +KF++GPALMAA S A+GL 
Sbjct: 241 SVRILSNGGLGMAMFSLGLFMASRPSIIACGTRMAAVAMALKFIVGPALMAASSFAIGLR 300

Query: 301 GRLLRVAIVQ 310
           G L ++A VQ
Sbjct: 301 GTLFKIAFVQ 310

[Close]

BLAST of Lsi03G014590 vs. TrEMBL
Match: V4SZ19_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10012051mg PE=4 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 2.8e-110
Identity = 216/310 (69.68%), Postives = 257/310 (82.90%), Query Frame = 1


Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MIS+ D YHVVAA VPLYVAM+LAYISVKWWK FT +QC+GINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISVVDAYHVVAACVPLYVAMLLAYISVKWWKLFTPDQCAGINKFVAKFSIPLLSFQVIS 60

Query: 61  NNNLYKMNLQLILADFLQKILAIVLLGAITRITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            NN YKMNL+LI ADFLQK+LA ++L A+T+I+SR GL WIITGLSLS++PN+LILG+P+
Sbjct: 61  KNNPYKMNLKLIYADFLQKLLAFLVLVALTKISSRSGLRWIITGLSLSSLPNSLILGIPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEA-TEIDITQDGRP 180
           L+AMY  EA +LLAQIVVLQS++WYN+LLFLFE    K+AS+ P  E   E D +Q+ + 
Sbjct: 121 LRAMYSEEAALLLAQIVVLQSLIWYNLLLFLFELNAAKSASVTPNPETPEEHDASQESQA 180

Query: 181 KEEIEEARDRNGRSLKTRSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDR 240
           KE  EEA++R    ++T  ILLTV RKLIINPNTHAT+LGLIWASI FRWGVKLP +ID+
Sbjct: 181 KEGEEEAQNRTATRVRTTHILLTVGRKLIINPNTHATLLGLIWASIEFRWGVKLPAIIDQ 240

Query: 241 SISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLG 300
           S+ ILS GGLGMAMFSLGLFM SR SIIACGT+M  VAM +KF++GPALMAA S A+GL 
Sbjct: 241 SVRILSNGGLGMAMFSLGLFMASRPSIIACGTRMAAVAMALKFIVGPALMAASSFAIGLR 300

Query: 301 GRLLRVAIVQ 310
           G L ++A VQ
Sbjct: 301 GTLFKIAFVQ 310

[Close]

BLAST of Lsi03G014590 vs. TAIR10
Match: AT5G15100.1 (AT5G15100.1 Auxin efflux carrier family protein)

HSP 1 Score: 317.0 bits (811), Expect = 1.5e-86
Identity = 179/320 (55.94%), Postives = 233/320 (72.81%), Query Frame = 1


Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MIS  D+YHVV+ATVPLYV+M L ++S +  K F+ EQC+GINKFVAKFSIPLLSFQ+IS
Sbjct: 1   MISWLDIYHVVSATVPLYVSMTLGFLSARHLKLFSPEQCAGINKFVAKFSIPLLSFQIIS 60

Query: 61  NNNLYKMNLQLILADFLQKILAIVLLGAITRIT----SRGG-LNWIITGLSLSTMPNTLI 120
            NN +KM+ +LIL+D LQK L +V+L  + R       RGG L W+ITGLS+S +PNTLI
Sbjct: 61  ENNPFKMSPKLILSDILQKFLVVVVLAMVLRFWHPTGGRGGKLGWVITGLSISVLPNTLI 120

Query: 121 LGLPVLKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPAS------EAT 180
           LG+P+L A+YG EA  +L QIVVLQS++WY ILLFLFE    +A   + AS      +  
Sbjct: 121 LGMPILSAIYGDEAASILEQIVVLQSLIWYTILLFLFELNAARALPSSGASLEHTGNDQE 180

Query: 181 EIDITQDGRPKEEIEEARDRNGRSLKTRSILLTVARKLIINPNTHATILGLIWASIRFRW 240
           E +I  + + +E+ EE      RS+ T  ILL   RKLIINPNT+AT++G+IWA++ FR 
Sbjct: 181 EANIEDEPKEEEDEEEVAIVRTRSVGTMKILLKAWRKLIINPNTYATLIGIIWATLHFRL 240

Query: 241 GVKLPEVIDRSISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALM 300
           G  LPE+ID+SI +LS GGLGMAMFSLGLFM S++SIIACGTKM ++ M +KF++GPALM
Sbjct: 241 GWNLPEMIDKSIHLLSDGGLGMAMFSLGLFMASQSSIIACGTKMAIITMLLKFVLGPALM 300

Query: 301 AACSLALGLGGRLLRVAIVQ 310
            A +  + L   L +VAI+Q
Sbjct: 301 IASAYCIRLKSTLFKVAILQ 320

[Close]

BLAST of Lsi03G014590 vs. TAIR10
Match: AT5G16530.1 (AT5G16530.1 Auxin efflux carrier family protein)

HSP 1 Score: 214.5 bits (545), Expect = 1.0e-55
Identity = 123/309 (39.81%), Postives = 188/309 (60.84%), Query Frame = 1


Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MI+  DVY V+ A VPLYVA++L Y SVKWW  FT +QC  IN+ V  F++PL + +  +
Sbjct: 1   MINCGDVYKVIEAMVPLYVALILGYGSVKWWHIFTRDQCDAINRLVCYFTLPLFTIEFTA 60

Query: 61  NNNLYKMNLQLILADFLQKILAIVLLGAITRITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           + + + MN + I AD L K++ + +L    + +++G   W IT  SL T+ N+L++G+P+
Sbjct: 61  HVDPFNMNYRFIAADVLSKVIIVTVLALWAKYSNKGSYCWSITSFSLCTLTNSLVVGVPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIDITQDGRPK 180
            KAMYG +A  L+ Q  V Q+I+W  +LLF+ EF   KA   +      ++D       K
Sbjct: 121 AKAMYGQQAVDLVVQSSVFQAIVWLTLLLFVLEF--RKAGFSSNNISDVQVDNINIESGK 180

Query: 181 EEIEEARDRNGRSLKTRSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRS 240
            E     +++        ++  V  KL  NPN ++ ILG+ WA I  RW ++LP +++ S
Sbjct: 181 RETVVVGEKS-----FLEVMSLVWLKLATNPNCYSCILGIAWAFISNRWHLELPGILEGS 240

Query: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLGG 300
           I I+S  G G AMF++G+FM  +  +I CGT +T++ M +KF+ GPA MA  S+ LGL G
Sbjct: 241 ILIMSKAGTGTAMFNMGIFMALQEKLIVCGTSLTVMGMVLKFIAGPAAMAIGSIVLGLHG 300

Query: 301 RLLRVAIVQ 310
            +LRVAI+Q
Sbjct: 301 DVLRVAIIQ 302

[Close]

BLAST of Lsi03G014590 vs. TAIR10
Match: AT1G73590.1 (AT1G73590.1 Auxin efflux carrier family protein)

HSP 1 Score: 175.6 bits (444), Expect = 5.2e-44
Identity = 99/202 (49.01%), Postives = 136/202 (67.33%), Query Frame = 1


Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MI+ +D YHV+ A VPLYVAM+LAY SVKWWK FT +QCSGIN+FVA F++PLLSF  I+
Sbjct: 1   MITAADFYHVMTAMVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIA 60

Query: 61  NNNLYKMNLQLILADFLQKILAIVLLGAITRITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            NN Y MNL+ + AD LQK++ + LL    +++  G L+W IT  SLST+PNTL++G+P+
Sbjct: 61  ANNPYAMNLRFLAADSLQKVIVLSLLFLWCKLSRNGSLDWTITLFSLSTLPNTLVMGIPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTK----------AASLAPASEATE 180
           LK MYG+ +  L+ QIVVLQ I+WY ++LFLFE+   K          A S+      ++
Sbjct: 121 LKGMYGNFSGDLMVQIVVLQCIIWYTLMLFLFEYRGAKLLISEQFPDTAGSIVSIHVDSD 180

Query: 181 IDITQDGRPKEEIEEARDRNGR 193
           I ++ DGR   E E     +G+
Sbjct: 181 I-MSLDGRQPLETEAEIKEDGK 201

[Close]

BLAST of Lsi03G014590 vs. TAIR10
Match: AT2G01420.2 (AT2G01420.2 Auxin efflux carrier family protein)

HSP 1 Score: 175.3 bits (443), Expect = 6.8e-44
Identity = 90/158 (56.96%), Postives = 118/158 (74.68%), Query Frame = 1


Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MI+  D+Y V+ A VPLYVAM+LAY SV+WWK F+ +QCSGIN+FVA F++PLLSF  IS
Sbjct: 1   MITWHDLYTVLTAVVPLYVAMILAYGSVQWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  NNNLYKMNLQLILADFLQKILAIVLLGAITRITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            N+ Y MN + + AD LQKI+ +VLL     +T  G L W+IT  SLST+PNTL++G+P+
Sbjct: 61  TNDPYAMNFRFVAADTLQKIIMLVLLALWANLTKNGSLEWMITIFSLSTLPNTLVMGIPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTK 159
           L AMYG+ A  L+ Q+VVLQ I+WY +LLFLFE+   K
Sbjct: 121 LIAMYGTYAGSLMVQVVVLQCIIWYTLLLFLFEYRGAK 158

[Close]

BLAST of Lsi03G014590 vs. TAIR10
Match: AT1G70940.1 (AT1G70940.1 Auxin efflux carrier family protein)

HSP 1 Score: 174.1 bits (440), Expect = 1.5e-43
Identity = 93/158 (58.86%), Postives = 115/158 (72.78%), Query Frame = 1


Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MIS  D+Y V+ A +PLYVAM+LAY SV+WWK F+ +QCSGIN+FVA F++PLLSF  IS
Sbjct: 1   MISWHDLYTVLTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  NNNLYKMNLQLILADFLQKILAIVLLGAITRITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            NN Y MNL+ I AD LQKI+ + LL      T  G L W IT  SLST+PNTL++G+P+
Sbjct: 61  TNNPYAMNLRFIAADTLQKIIMLSLLVLWANFTRSGSLEWSITIFSLSTLPNTLVMGIPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTK 159
           L AMYG  +  L+ QIVVLQ I+WY +LLFLFEF   K
Sbjct: 121 LIAMYGEYSGSLMVQIVVLQCIIWYTLLLFLFEFRGAK 158

[Close]

BLAST of Lsi03G014590 vs. NCBI nr
Match: gi|659085474|ref|XP_008443438.1| (PREDICTED: putative auxin efflux carrier component 5 isoform X2 [Cucumis melo])

HSP 1 Score: 554.3 bits (1427), Expect = 1.5e-154
Identity = 299/309 (96.76%), Postives = 306/309 (99.03%), Query Frame = 1


Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  NNNLYKMNLQLILADFLQKILAIVLLGAITRITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           +NNLYKMNLQLILADFLQKILAI+LLGAIT+ITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  DNNLYKMNLQLILADFLQKILAIILLGAITKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIDITQDGRPK 180
           LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEI+ITQ+GR K
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITQEGRAK 180

Query: 181 EEIEEARDRNGRSLKTRSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRS 240
           EEIEEARDRNGRSLKT+SILLTVARKLIINPNTHATILGLIW+SIRFRWGVKLP VIDRS
Sbjct: 181 EEIEEARDRNGRSLKTKSILLTVARKLIINPNTHATILGLIWSSIRFRWGVKLPVVIDRS 240

Query: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLGG 300
           ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGL G
Sbjct: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLRG 300

Query: 301 RLLRVAIVQ 310
           RLLRVAIVQ
Sbjct: 301 RLLRVAIVQ 309

[Close]

BLAST of Lsi03G014590 vs. NCBI nr
Match: gi|778685606|ref|XP_011652244.1| (PREDICTED: putative auxin efflux carrier component 5 [Cucumis sativus])

HSP 1 Score: 552.4 bits (1422), Expect = 5.8e-154
Identity = 296/309 (95.79%), Postives = 305/309 (98.71%), Query Frame = 1


Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  NNNLYKMNLQLILADFLQKILAIVLLGAITRITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            NNLYKMN QLILADFLQKILAI+LLGA+T+ITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIDITQDGRPK 180
           LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEI+IT +GRPK
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITHEGRPK 180

Query: 181 EEIEEARDRNGRSLKTRSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRS 240
           EEIEEARDRNGRSL+T+SILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRS
Sbjct: 181 EEIEEARDRNGRSLRTKSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRS 240

Query: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLGG 300
           ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFL+GPALMAACSLALGL G
Sbjct: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLVGPALMAACSLALGLRG 300

Query: 301 RLLRVAIVQ 310
           +LLRVAIVQ
Sbjct: 301 KLLRVAIVQ 309

[Close]

BLAST of Lsi03G014590 vs. NCBI nr
Match: gi|659085472|ref|XP_008443437.1| (PREDICTED: putative auxin efflux carrier component 5 isoform X1 [Cucumis melo])

HSP 1 Score: 549.7 bits (1415), Expect = 3.8e-153
Identity = 299/310 (96.45%), Postives = 306/310 (98.71%), Query Frame = 1


Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  NNNLYKMNLQLILADFLQKILAIVLLGAITRITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           +NNLYKMNLQLILADFLQKILAI+LLGAIT+ITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  DNNLYKMNLQLILADFLQKILAIILLGAITKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEAT-EIDITQDGRP 180
           LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEAT EI+ITQ+GR 
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATAEIEITQEGRA 180

Query: 181 KEEIEEARDRNGRSLKTRSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDR 240
           KEEIEEARDRNGRSLKT+SILLTVARKLIINPNTHATILGLIW+SIRFRWGVKLP VIDR
Sbjct: 181 KEEIEEARDRNGRSLKTKSILLTVARKLIINPNTHATILGLIWSSIRFRWGVKLPVVIDR 240

Query: 241 SISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLG 300
           SISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGL 
Sbjct: 241 SISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLR 300

Query: 301 GRLLRVAIVQ 310
           GRLLRVAIVQ
Sbjct: 301 GRLLRVAIVQ 310

[Close]

BLAST of Lsi03G014590 vs. NCBI nr
Match: gi|1009125051|ref|XP_015879402.1| (PREDICTED: putative auxin efflux carrier component 5 isoform X2 [Ziziphus jujuba])

HSP 1 Score: 450.7 bits (1158), Expect = 2.4e-123
Identity = 241/333 (72.37%), Postives = 279/333 (83.78%), Query Frame = 1


Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MIS +DVYHVVAAT+PLYVAM+LAYISVKWW+FFT +QCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISPTDVYHVVAATIPLYVAMILAYISVKWWQFFTPDQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  NNNLYKMNLQLILADFLQKILAIVLLGAITRITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            NN YKMNL+LIL+DFLQK+ A V L AITRI+SRGGLNW ITGLSLST+PNTLILG+P+
Sbjct: 61  ANNPYKMNLKLILSDFLQKLFAFVALTAITRISSRGGLNWTITGLSLSTLPNTLILGIPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATE-IDITQDGRP 180
           LKAMYG EA   LAQIVVLQSI+WYNILLFLFEF   K+AS++P+SE TE ++   + + 
Sbjct: 121 LKAMYGEEAAGNLAQIVVLQSIIWYNILLFLFEFNAVKSASVSPSSETTEDMEAPHEAQS 180

Query: 181 KEEIEEARDRNGRSLKTRSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDR 240
           KEE+EEAR R  R +KT  ILLTV RKL+ NPNTHAT++GLIWA IRFRW +KLPE+ ++
Sbjct: 181 KEEVEEARTRTPRKIKTMLILLTVGRKLMSNPNTHATVIGLIWAVIRFRWNIKLPEIANQ 240

Query: 241 SISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLG 300
           SI ILS GGLGMAMFSLGLFM SRT IIACGT++ ++AM MKF++GPALMA  S A+GL 
Sbjct: 241 SIIILSNGGLGMAMFSLGLFMASRTRIIACGTRLAILAMAMKFIIGPALMAGASYAVGLS 300

Query: 301 GRLLRVAIVQGTFGLAHCTASSTGLLLLAVIIK 333
           G +LRVAIVQG F  AHC A  T LLLL  IIK
Sbjct: 301 GTILRVAIVQGNFWAAHCLAHRTDLLLLVSIIK 333

[Close]

BLAST of Lsi03G014590 vs. NCBI nr
Match: gi|1009125049|ref|XP_015879401.1| (PREDICTED: putative auxin efflux carrier component 5 isoform X1 [Ziziphus jujuba])

HSP 1 Score: 427.9 bits (1099), Expect = 1.7e-116
Identity = 227/310 (73.23%), Postives = 265/310 (85.48%), Query Frame = 1


Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MIS +DVYHVVAAT+PLYVAM+LAYISVKWW+FFT +QCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISPTDVYHVVAATIPLYVAMILAYISVKWWQFFTPDQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  NNNLYKMNLQLILADFLQKILAIVLLGAITRITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            NN YKMNL+LIL+DFLQK+ A V L AITRI+SRGGLNW ITGLSLST+PNTLILG+P+
Sbjct: 61  ANNPYKMNLKLILSDFLQKLFAFVALTAITRISSRGGLNWTITGLSLSTLPNTLILGIPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATE-IDITQDGRP 180
           LKAMYG EA   LAQIVVLQSI+WYNILLFLFEF   K+AS++P+SE TE ++   + + 
Sbjct: 121 LKAMYGEEAAGNLAQIVVLQSIIWYNILLFLFEFNAVKSASVSPSSETTEDMEAPHEAQS 180

Query: 181 KEEIEEARDRNGRSLKTRSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDR 240
           KEE+EEAR R  R +KT  ILLTV RKL+ NPNTHAT++GLIWA IRFRW +KLPE+ ++
Sbjct: 181 KEEVEEARTRTPRKIKTMLILLTVGRKLMSNPNTHATVIGLIWAVIRFRWNIKLPEIANQ 240

Query: 241 SISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLG 300
           SI ILS GGLGMAMFSLGLFM SRT IIACGT++ ++AM MKF++GPALMA  S A+GL 
Sbjct: 241 SIIILSNGGLGMAMFSLGLFMASRTRIIACGTRLAILAMAMKFIIGPALMAGASYAVGLS 300

Query: 301 GRLLRVAIVQ 310
           G +LRVAIVQ
Sbjct: 301 GTILRVAIVQ 310

The following BLAST results are available for this feature:

BLAST of Lsi03G014590 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PIN8_ARATH	2.6e-85	55.94	Auxin efflux carrier component 8 OS=Arabidopsis thaliana GN=PIN8 PE=2 SV=1	[more]
PIN6_ORYSJ	1.2e-55	39.87	Probable auxin efflux carrier component 6 OS=Oryza sativa subsp. japonica GN=PIN...	[more]
PIN5_ARATH	1.8e-54	39.81	Auxin efflux carrier component 5 OS=Arabidopsis thaliana GN=PIN5 PE=2 SV=2	[more]
PIN4_ORYSJ	1.3e-52	38.46	Probable auxin efflux carrier component 4 OS=Oryza sativa subsp. japonica GN=PIN...	[more]
PIN1_ORYSJ	1.7e-44	58.44	Auxin efflux carrier component 1 OS=Oryza sativa subsp. japonica GN=PIN1 PE=2 SV...	[more]

BLAST of Lsi03G014590 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LFB7_CUCSA	4.1e-154	95.79	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G827360 PE=4 SV=1	[more]
W9QE17_9ROSA	4.5e-113	70.29	Putative auxin efflux carrier component 5 OS=Morus notabilis GN=L484_001515 PE=4...	[more]
M5WVA2_PRUPE	5.0e-112	71.47	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021573mg PE=4 SV=1	[more]
A0A067FYK2_CITSI	2.8e-110	69.68	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g018360mg PE=4 SV=1	[more]
V4SZ19_9ROSI	2.8e-110	69.68	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10012051mg PE=4 SV=1	[more]

BLAST of Lsi03G014590 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G15100.1	1.5e-86	55.94	Auxin efflux carrier family protein	[more]
AT5G16530.1	1.0e-55	39.81	Auxin efflux carrier family protein	[more]
AT1G73590.1	5.2e-44	49.01	Auxin efflux carrier family protein	[more]
AT2G01420.2	6.8e-44	56.96	Auxin efflux carrier family protein	[more]
AT1G70940.1	1.5e-43	58.86	Auxin efflux carrier family protein	[more]

BLAST of Lsi03G014590 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659085474\|ref\|XP_008443438.1\|	1.5e-154	96.76	PREDICTED: putative auxin efflux carrier component 5 isoform X2 [Cucumis melo]	[more]
gi\|778685606\|ref\|XP_011652244.1\|	5.8e-154	95.79	PREDICTED: putative auxin efflux carrier component 5 [Cucumis sativus]	[more]
gi\|659085472\|ref\|XP_008443437.1\|	3.8e-153	96.45	PREDICTED: putative auxin efflux carrier component 5 isoform X1 [Cucumis melo]	[more]
gi\|1009125051\|ref\|XP_015879402.1\|	2.4e-123	72.37	PREDICTED: putative auxin efflux carrier component 5 isoform X2 [Ziziphus jujuba...	[more]
gi\|1009125049\|ref\|XP_015879401.1\|	1.7e-116	73.23	PREDICTED: putative auxin efflux carrier component 5 isoform X1 [Ziziphus jujuba...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0055085	transmembrane transport

Vocabulary: **Cellular Component**
Term	Definition
GO:0016021	integral component of membrane

Vocabulary: **INTERPRO**
Term	Definition
IPR014024	Auxin_eff_plant
IPR004776	Mem_trans

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010315	auxin efflux
biological_process	GO:0010252	auxin homeostasis
biological_process	GO:0009555	pollen development
biological_process	GO:0055085	transmembrane transport
biological_process	GO:0009734	auxin-activated signaling pathway
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G014590.1	Lsi03G014590.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004776	Membrane transport protein	PFAM	PF03547	Mem_trans	coord: 9..324 score: 7.8
IPR014024	Auxin efflux carrier, plant type	TIGRFAMs	TIGR00946	TIGR00946	coord: 1..310 score: 1.7
None	No IPR available	PANTHER	PTHR31752	FAMILY NOT NAMED	coord: 2..309 score: 2.8E
None	No IPR available	PANTHER	PTHR31752:SF10	AUXIN EFFLUX CARRIER COMPONENT 5-RELATED	coord: 2..309 score: 2.8E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi03G014590	Csa3G827360	Cucumber (Chinese Long) v2	culsiB186
Lsi03G014590	CSPI03G39240	Wild cucumber (PI 183967)	cpilsiB184
Lsi03G014590	Cucsa.339090	Cucumber (Gy14) v1	cgylsiB551
Lsi03G014590	CmaCh14G006050	Cucurbita maxima (Rimu)	cmalsiB242
Lsi03G014590	CmoCh14G006160	Cucurbita moschata (Rifu)	cmolsiB230
Lsi03G014590	Cla017028	Watermelon (97103) v1	lsiwmB240
Lsi03G014590	MELO3C009892	Melon (DHL92) v3.5.1	lsimeB260
Lsi03G014590	ClCG10G014820	Watermelon (Charleston Gray)	lsiwcgB219
Lsi03G014590	MELO3C009892.2	Melon (DHL92) v3.6.1	lsimedB273
Lsi03G014590	Cla97C10G197710	Watermelon (97103) v2	lsiwmbB218
Lsi03G014590	Bhi11G002525	Wax gourd	lsiwgoB300
Lsi03G014590	Cp4.1LG04g01350	Cucurbita pepo (Zucchini)	cpelsiB547
Lsi03G014590	Cp4.1LG03g08040	Cucurbita pepo (Zucchini)	cpelsiB503
Lsi03G014590	CsGy3G036840	Cucumber (Gy14) v2	cgyblsiB171
Lsi03G014590	Carg22907	Silver-seed gourd	carlsiB191
Lsi03G014590	Carg09772	Silver-seed gourd	carlsiB394
Lsi03G014590	CsaV3_3G041710	Cucumber (Chinese Long) v3	cuclsiB203

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi03G014590	Bottle gourd (USVL1VR-Ls)	lsilsiB094
Lsi03G014590	Cucumber (Gy14) v1	cgylsiB255
Lsi03G014590	Cucumber (Gy14) v1	cgylsiB463
Lsi03G014590	Cucurbita maxima (Rimu)	cmalsiB119
Lsi03G014590	Cucurbita maxima (Rimu)	cmalsiB658
Lsi03G014590	Cucurbita moschata (Rifu)	cmolsiB107
Lsi03G014590	Cucurbita moschata (Rifu)	cmolsiB647
Lsi03G014590	Cucurbita pepo (Zucchini)	cpelsiB145
Lsi03G014590	Wild cucumber (PI 183967)	cpilsiB359
Lsi03G014590	Wild cucumber (PI 183967)	cpilsiB360
Lsi03G014590	Watermelon (Charleston Gray)	lsiwcgB244
Lsi03G014590	Cucumber (Chinese Long) v2	culsiB356
Lsi03G014590	Cucumber (Chinese Long) v2	culsiB355
Lsi03G014590	Melon (DHL92) v3.5.1	lsimeB237
Lsi03G014590	Watermelon (Charleston Gray)	lsiwcgB216
Lsi03G014590	Watermelon (97103) v1	lsiwmB236
Lsi03G014590	Watermelon (97103) v1	lsiwmB257
Lsi03G014590	Cucumber (Gy14) v2	cgyblsiB328
Lsi03G014590	Melon (DHL92) v3.6.1	lsimedB245
Lsi03G014590	Melon (DHL92) v3.6.1	lsimedB250
Lsi03G014590	Silver-seed gourd	carlsiB238
Lsi03G014590	Cucumber (Chinese Long) v3	cuclsiB381
Lsi03G014590	Cucumber (Chinese Long) v3	cuclsiB383
Lsi03G014590	Watermelon (97103) v2	lsiwmbB252
Lsi03G014590	Wax gourd	lsiwgoB313
Lsi03G014590	Wax gourd	lsiwgoB340