Lsi03G012770 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi03G012770
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionCytochrome P450 family protein
Locationchr03 : 23557615 .. 23562649 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TGAGCAAAATGTGGGTTGTTATGGCTGCTATTTTTGGTTTTAGTGTTATAATTTGGCTCTATGGTTTGAAATATTCAAAATATTATGGAAAACTTCCAAAAGGTTCAATGGGATTTCCCATAATTGGTGAATCAAGGCAGTTCTTTGCTCCTAATCCATCTTTTGATTTATCTCCTTTCATCAAAGACAGAATTCTCAAGTATGACAATGTTTTATTTTTTTTTTCCTTTTTGTATATTAAGAAATTAAACCATCTATATTATTCTTTCTTCTTTTTTTTTTTTTTTTGAATTTTGGTATTTTTGTTTCAGGTATGGACCAATTTTCAAAACAAGATTAGTGGGGAAACCATTAATTATTTCAGCAGATCCTGAACTGAATTATATTATATTTCAACAAGAGGAACAATTGTTTGAGTGTTGGTATCCAGAGACCTTCAGAAAGATATTTGGAGGACAATTTGTAGGGTCCTTGCATGGTTTTATGCATAAATATTTGAAGAACATGATTATTAATGTTTTTGGAATTGAAAGTTTGAAAAATATGATTTTTGAAGTTGAAGTTGCAGCAACTACAAGATTGAAGAAATGGGCATCTCATAATACAGTTGAATTGAAGGATGAGATTGCAAATGTAAATTTATATTGAAAAAAAAAAAACTTGTTTTAATTATTTGACATGTTCAATCAAGGTTTTAATTTTTCTTTTTTACCTTATTAATATTAATTTGGTTCCATGCAGATGATATTTGATCTCACTGCAAAGAGATTAATTAGTTATGATCCAAAAAAGTCTTCAGAAAATTTGAGAGAGAACTTTGTTGCCTTCATTCAGGGTTTGATCTCTTTCCCTCTTGATATTCCTGGAACCTCTTTTCACAAATGCTTACAGGTAACTTTTTATTTATTTATTTAGTTGTGGTTCCTTAAATTACTTGTGTCAAAAAAAAAAAAAAAAACTTGGTCGTTTTAGGGTTTTTCTTTCAAAATTAACCTATGAAAAACGGAAGATGAGAGTCTGATAGAAATTTATTTTTAGTAGGTTAAAGAATTATAAATTTTTTATTTTTAAAAGGCTAAATAGCACAGTTTGAAGTTACGTGCCACCCTAAATTTTTAAAAAAAAAATCATTTTCACCTTTCAATTTCGAAATTATTCTAATAATATATGATTTTAGAGGGCCTTTTATTTATTTATTTATTTAAGATGTACGAAGGTAAAAACGATTAGTGTGTAGTTGATTGTACGAATTGATCATATTATTTTCTAACTCATGTTTCAACTTTGTATAGGAAATTAAAAATATAATTCTAAATGATGTGATGTATGTTAGTCCAATTAATGTTACACATTAGTGTTGTTGTACTGTCAATTTCCACTCATCGTTATTTTTTTTTTGGATTGTTTTTGGGAATAATTTAAGAGTTCAAGAATTAAAAAATAATAATAAAATATAAAAAATCTCGATCAACAATAAAATGTACAAACTCAAATGACGATAAGTACATCTTGGATGCAAGATGCAACTATCTTATTGCAATTCTTTCTTCTTTCTTCTTTTTCTTAAACATCTTTGAATTTTTTATGTTGTATTGTTAGAGAAGGAAAGGGGGTAATTTATGATATACACTCTAGCCACTTGGTTCCCAGACATCCTCAATTTTTATAGGGGAACATTTAGGGACTCATTTGATAACGTTTCTATTTTCTATTTCCTGTTTTTGTTTCTCATTTTTTAAGAAGCTGACTTGTTTGATAATCGTTCTTGTTTCTTGTGTTTAGAATTTGAGAAACGTTTCTAAAAATGGGGCCAAATTTGAGAAACTGTTCCATTTCTATTTTATATAATGTTGTTTCTTTTATTTATCAAAACTCATTTTTGACATATTGGTAGTCATGCATGTCTATACTACTGTTTTACCTTAAACGGACCCTAACGTTTCCGTTTCTTGTTTCTCATTTTTTAAGAAACCGACTTGTTTGATAACTGCTCTCGTTTTCTGTTTCTAGAATTTGAGAAATGTTTTTGAAAATGGGGTCAAATTTGAGAAATTACAAAAAGTAGCTTCTCTCTGTTTCATTTCTGTTGTATACATTATTATTATTATTATTATTTTTATCAAAACTCATCATTAGCCTACTGGTAGTCATGCACACTTATACTAAATATTTGGACTTTCATGTTTTCATATTTTAGAAATAGCACATTGTTCAAAACTTGTTAAAAGAGTTTTTTTTTTCGATCATCTCAGCCCAGATCGACTACCGAGATTTATATTAAAAAATTAACTTTTCATCTTATCTAATATATTTTGGGCTTTCTCGGAGAAATCTCGGGCATGATTAACACCGAGATTTTATATATTTTCCAAATCTTACATAATTTTTTTTGCAAAATTTGATCTGTTCCAAAATTTGAAAACAATATCCATTTAGAAAATCCACTTAGATAATTTGTAATTCCATTTGAAAATTTGAAATTTAGAAATTTGAAACAGAAATTGGGTGAAAGATTTGAAAATTTTTATATAAGATTTGGTAAAGAAAACAAATTTTGAAGAAAATTTGAAAAGATGTAAATTTTGGTATAACTTTTGGAAATATGACGTGATATTTAGAAAAAAAAATAGGAGAATCTCGTTGTTCATCCGTACGAGATTTTACTAAGAAAGTCCAAAACAATCGATATTAAATGAAAAAGTTAACTTTTTTTTTTTCAAATTTCAGTAGTCGATCTCGACCGAGATGGACCGAATCAAACTATTTTAACGAATTTTTTAAAAGGATGCTAGTTGTGAAAGAGAAACATTTCATATTATTTCTAACCATTGAATTTGAGAATTTATATACTTTTTTTTGACAGGGTAGAAAAAGAGTAATTAGGATGCTGGAAAACATGCTACATGAAAGGCAGCAAAATCCAAGGAAACAACATATTGATTATTTTGATTATGTTCTTCAAGAACTCCAAAAAGATGGAACTCCTCTCACCCAAGAAATTGCTTTGGACTTGATCTTTTTGCTTCTATTTGCAAGCTATGAAACCACTTCAATTGCTTTGACTTTAGCCATCAAATTTCTCATAGACCACCCCCACGTCTTGAACGAGTTAACGGTGCATTCCTTTTTAGATGTATCTTTGGGTTTGAAATGATCTTTAAAAAAAAACAAAAACAAATTTAGTTTCATCACATCCCTTGTTAACATATTTAATTTCCATGCAAAATCTTGATCATCAGGCAGAACATGAAAGAATTTTGAAGGAGAGGAAGAATAAAAGTGGTGGGATTACCTGGAACGAATACAAATCCATGACATTTACATTCAAGGTCAACGAAGCATTCGGAAAATTATTTAGTTGTGTTATTGTTAATGATAGTTTGAAATTAGTTAATTAACAAGTGGAAAACTTAATTAATATAGTTTCTAAACGAAACATTGAGGTTGGCAAATATTGCTCCAGGTATATTTCGAAGAGCATTAAAAGATGTTGAGTTTAAGGGTAAGGAAAAATAATATGGTGTGTTAGATTTTAATAAATTACTTATTTAAATTATAAGTGGATTAAATTTTATATATATGGTTGCACAGGATACACAATTCCAGAAGGATGGGCTGTTATGGTTTGCCCTCCAGCCATTCACTTACATCCAAAAAATTACATTGATCCACTTGCCTTCAACCCATGGCGATGGGAGGTACCTTATTCAATTCAATATAATATATATATATATATATATATATTTTGACAATATGTAGAGAGGAAAGATCAAACATCTAATTTTGAACTTGATAGTACAAGTACTATGCTAATTGAACTATGCTCACACTTTGGCAATTTAATAATATTGTTGTTTTTAAATATGGAAAAATAAGTTGAACTATTTACAAATATAGAAAAAAATTTGCGATAGAAAGTTAAATTTTTTCTATATTTGTAAATAGTTTGATATTTTTTTTGTTTATAGTAATTTCCCTTAATAATATGTATCTTAATACCCCTCGCTTATATATATATATATATATATATATATATATATATATATATATATATATATATATATTTCTTTTCTTTTTTTTTTTGTTAGAAATATTCACAAGTTTGTTATCAAACATTACATAGTTGAATTACAAAACTCCTAGATGATCAACAATAACATATTTGAAAATGATTTTTAAATGATCAAAATTATTTTTGAGGTGTCCAAAATTACTTTTCTTCTAAAATTTTGATGTTTGACACTGATGAGAAAATGATTTTGAAAACATCCAAAATTACTTAAAGTTGTGATTCACTATCAACTACTTGGAAAATAATTCTAAAGTGATTTTTGTTAGACGATTGTGAAGGGTATTTTTACTATTATTTCATATTTAAAAATTATGCTACTATTTCACTTTATTTTGTCCTAAAACAGTTTTTTTTAAATTTGTGTCCATTTTTATCTTTATTTCTCTCTAATTGTTTACCCTCTCCCTACTTTTCTGTAATGTTTAACTCTAATTTTATACTTTTTTTAAAAAAAAAAAATAATTTCTCACCTTTTCTCTCTCTAGTGGTTTTTAACTCTCTCTAGTTTTTTTATCTCGAAATTATTATATTATATTAATTAATAATAATAAGCAGAGATGTGATGGGGCATCGAAGCATTTCATGGCATTTGGTGGAGGAATGAGATTTTGCATTGGTGCAGACTTTGCCAAAATGCAAATGGCTGTGTTTCTCCATAACTTGGTTACCAATTACACGTAATTAATCTCTCTTATTAATTACCTTCTAAATTCCCATTTATATTAAAATAAACAATTAAAATTTATTTTTTATATTTTTTTATGCATATATTATCGTAGGGAGAGATGATTTAATGTAGTTTTTTCTTATATGGCATTTTTTACACTTAGGTTGAAGGCAATTAAAGGAGGCAATATTGTTCGAACCCCAGGCGTACAATTTCCCGATGGCTTTCATGTTGAGATCGTTAAAAAAAAAGTAGTAGCCCTCCAAATACTAACCAAAACCTATAAGTCTTCTAAA

mRNA sequence

TGAGCAAAATGTGGGTTGTTATGGCTGCTATTTTTGGTTTTAGTGTTATAATTTGGCTCTATGGTTTGAAATATTCAAAATATTATGGAAAACTTCCAAAAGGTTCAATGGGATTTCCCATAATTGGTGAATCAAGGCAGTTCTTTGCTCCTAATCCATCTTTTGATTTATCTCCTTTCATCAAAGACAGAATTCTCAAGTATGGACCAATTTTCAAAACAAGATTAGTGGGGAAACCATTAATTATTTCAGCAGATCCTGAACTGAATTATATTATATTTCAACAAGAGGAACAATTGTTTGAGTGTTGGTATCCAGAGACCTTCAGAAAGATATTTGGAGGACAATTTGTAGGGTCCTTGCATGGTTTTATGCATAAATATTTGAAGAACATGATTATTAATGTTTTTGGAATTGAAAGTTTGAAAAATATGATTTTTGAAGTTGAAGTTGCAGCAACTACAAGATTGAAGAAATGGGCATCTCATAATACAGTTGAATTGAAGGATGAGATTGCAAATATGATATTTGATCTCACTGCAAAGAGATTAATTAGTTATGATCCAAAAAAGTCTTCAGAAAATTTGAGAGAGAACTTTGTTGCCTTCATTCAGGGTTTGATCTCTTTCCCTCTTGATATTCCTGGAACCTCTTTTCACAAATGCTTACAGCAAAATCCAAGGAAACAACATATTGATTATTTTGATTATGTTCTTCAAGAACTCCAAAAAGATGGAACTCCTCTCACCCAAGAAATTGCTTTGGACTTGATCTTTTTGCTTCTATTTGCAAGCTATGAAACCACTTCAATTGCTTTGACTTTAGCCATCAAATTTCTCATAGACCACCCCCACGTCTTGAACGAGTTAACGGTGCATTCCTTTTTAGATGTATCTTTGGTTTCATCACATCCCTTGTTAACATATTTAATTTCCATGCAAAATCTTGATCATCAGGCAGAACATGAAAGAATTTTGAAGGAGAGGAAGAATAAAAGTGGTGGGATTACCTGGAACGAATACAAATCCATGACATTTACATTCAAGGTCAACGAAGCATTCGGAAAATTATTTAGTTGTGTTATTTTTCTAAACGAAACATTGAGGTTGGCAAATATTGCTCCAGGTATATTTCGAAGAGCATTAAAAGATGTTGAGTTTAAGGGATACACAATTCCAGAAGGATGGGCTGTTATGAGATGTGATGGGGCATCGAAGCATTTCATGGCATTTGGTGGAGGAATGAGATTTTGCATTGGTGCAGACTTTGCCAAAATGCAAATGGCTGTGTTTCTCCATAACTTGGTTACCAATTACACGTTGAAGGCAATTAAAGGAGGCAATATTGTTCGAACCCCAGGCGTACAATTTCCCGATGGCTTTCATGTTGAGATCGTTAAAAAAAAAGTAGTAGCCCTCCAAATACTAACCAAAACCTATAAGTCTTCTAAA

Coding sequence (CDS)

ATGTGGGTTGTTATGGCTGCTATTTTTGGTTTTAGTGTTATAATTTGGCTCTATGGTTTGAAATATTCAAAATATTATGGAAAACTTCCAAAAGGTTCAATGGGATTTCCCATAATTGGTGAATCAAGGCAGTTCTTTGCTCCTAATCCATCTTTTGATTTATCTCCTTTCATCAAAGACAGAATTCTCAAGTATGGACCAATTTTCAAAACAAGATTAGTGGGGAAACCATTAATTATTTCAGCAGATCCTGAACTGAATTATATTATATTTCAACAAGAGGAACAATTGTTTGAGTGTTGGTATCCAGAGACCTTCAGAAAGATATTTGGAGGACAATTTGTAGGGTCCTTGCATGGTTTTATGCATAAATATTTGAAGAACATGATTATTAATGTTTTTGGAATTGAAAGTTTGAAAAATATGATTTTTGAAGTTGAAGTTGCAGCAACTACAAGATTGAAGAAATGGGCATCTCATAATACAGTTGAATTGAAGGATGAGATTGCAAATATGATATTTGATCTCACTGCAAAGAGATTAATTAGTTATGATCCAAAAAAGTCTTCAGAAAATTTGAGAGAGAACTTTGTTGCCTTCATTCAGGGTTTGATCTCTTTCCCTCTTGATATTCCTGGAACCTCTTTTCACAAATGCTTACAGCAAAATCCAAGGAAACAACATATTGATTATTTTGATTATGTTCTTCAAGAACTCCAAAAAGATGGAACTCCTCTCACCCAAGAAATTGCTTTGGACTTGATCTTTTTGCTTCTATTTGCAAGCTATGAAACCACTTCAATTGCTTTGACTTTAGCCATCAAATTTCTCATAGACCACCCCCACGTCTTGAACGAGTTAACGGTGCATTCCTTTTTAGATGTATCTTTGGTTTCATCACATCCCTTGTTAACATATTTAATTTCCATGCAAAATCTTGATCATCAGGCAGAACATGAAAGAATTTTGAAGGAGAGGAAGAATAAAAGTGGTGGGATTACCTGGAACGAATACAAATCCATGACATTTACATTCAAGGTCAACGAAGCATTCGGAAAATTATTTAGTTGTGTTATTTTTCTAAACGAAACATTGAGGTTGGCAAATATTGCTCCAGGTATATTTCGAAGAGCATTAAAAGATGTTGAGTTTAAGGGATACACAATTCCAGAAGGATGGGCTGTTATGAGATGTGATGGGGCATCGAAGCATTTCATGGCATTTGGTGGAGGAATGAGATTTTGCATTGGTGCAGACTTTGCCAAAATGCAAATGGCTGTGTTTCTCCATAACTTGGTTACCAATTACACGTTGAAGGCAATTAAAGGAGGCAATATTGTTCGAACCCCAGGCGTACAATTTCCCGATGGCTTTCATGTTGAGATCGTTAAAAAAAAAGTAGTAGCCCTCCAAATACTAACCAAAACCTATAAGTCTTCTAAA

Protein sequence

MWVVMAAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIKDRILKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLHGFMHKYLKNMIINVFGIESLKNMIFEVEVAATTRLKKWASHNTVELKDEIANMIFDLTAKRLISYDPKKSSENLRENFVAFIQGLISFPLDIPGTSFHKCLQQNPRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPHVLNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYKSMTFTFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAVMRCDGASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVTNYTLKAIKGGNIVRTPGVQFPDGFHVEIVKKKVVALQILTKTYKSSK
BLAST of Lsi03G012770 vs. Swiss-Prot
Match: C87A3_ORYSJ (Cytochrome P450 87A3 OS=Oryza sativa subsp. japonica GN=CYP87A3 PE=2 SV=3)

HSP 1 Score: 471.9 bits (1213), Expect = 8.6e-132
Identity = 238/501 (47.50%), Postives = 329/501 (65.67%), Query Frame = 1

Query: 12  SVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIKDRILKYGPIFKT 71
           +++ W Y   + +  G+LP GS+G P+IGE+ QFFAPNP+ DLSPF+K+RI +YG IFKT
Sbjct: 54  ALLRWAYRWSHPRSNGRLPPGSLGLPVIGETLQFFAPNPTCDLSPFVKERIKRYGSIFKT 113

Query: 72  RLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLHGFMHKYLKNMII 131
            +VG+P+++SADPE+NY +FQQE +LFE WYP+TF +IFG   VGSLHGFM+KYLK +++
Sbjct: 114 SVVGRPVVVSADPEMNYYVFQQEGKLFESWYPDTFTEIFGRDNVGSLHGFMYKYLKTLVL 173

Query: 132 NVFGIESLKNMIF-EVEVAATTRLKKWASHNTVELKDEIANMIFDLTAKRLISYDPKKSS 191
            ++G E+LK+++  E + A    L  WAS  +VELK+ I+ MIFDLTAK+LI YDP K S
Sbjct: 174 RLYGQENLKSVLLAETDAACRGSLASWASQPSVELKEGISTMIFDLTAKKLIGYDPSKPS 233

Query: 192 E-NLRENFVAFIQGLISFPLDIPGTSFHKCLQ-----------------QNPRKQHIDYF 251
           + NLR+NF AFI GLISFPL+IPGT++H+C++                   P +   D+F
Sbjct: 234 QVNLRKNFGAFICGLISFPLNIPGTAYHECMEGRKNAMKVLRGMMKERMAEPERPCEDFF 293

Query: 252 DYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPHVLNELTVHSF 311
           D+V+QEL+++   LT+ IALDL+F+LLFAS+ETT++ALT+ +K L ++P V++ L     
Sbjct: 294 DHVIQELRREKPLLTETIALDLMFVLLFASFETTALALTIGVKLLTENPKVVDAL----- 353

Query: 312 LDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYKSMTFTFKVNEAFG 371
                                  + EHE I++ RK+ + G+TW EYKSMTFT +V     
Sbjct: 354 -----------------------REEHEAIIRNRKDPNSGVTWAEYKSMTFTSQV----- 413

Query: 372 KLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAVMRC-------------- 431
                   + E +RLANI PGIFR+AL+DVE KGYTIP GW +M C              
Sbjct: 414 --------IMEIVRLANIVPGIFRKALQDVEIKGYTIPAGWGIMVCPPAVHLNPEIYEDP 473

Query: 432 --------------DGASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVTNYTLKAIKGG 466
                          G +KHFMAFGGG+RFC+G D +K+ MA F+H+LVT Y+ + +KGG
Sbjct: 474 LAFNPWRWQGKPEITGGTKHFMAFGGGLRFCVGTDLSKVLMATFIHSLVTKYSWRTVKGG 513

BLAST of Lsi03G012770 vs. Swiss-Prot
Match: THAH_ARATH (Cytochrome P450 708A2 OS=Arabidopsis thaliana GN=CYP708A2 PE=2 SV=3)

HSP 1 Score: 259.2 bits (661), Expect = 8.8e-68
Identity = 165/508 (32.48%), Postives = 256/508 (50.39%), Query Frame = 1

Query: 1   MWVVMAAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIKD 60
           +WV+  A    S   WLY     K  GKLP GSMG PIIGE+  FF P+  +++SPF+K 
Sbjct: 9   VWVIAVAAVVISK--WLYRWSNPKCNGKLPPGSMGLPIIGETCDFFEPHGLYEISPFVKK 68

Query: 61  RILKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLHG 120
           R+LKYGP+F+T + G   ++  +P++ + +F+QE + F   YPE F K FG + V   HG
Sbjct: 69  RMLKYGPLFRTNIFGSNTVVLTEPDIIFEVFRQENKSFVFSYPEAFVKPFGKENVFLKHG 128

Query: 121 FMHKYLKNMIINVFGIESL-KNMIFEVEVAATTRLKKWASHNTVELKDEIANMIFDLTAK 180
            +HK++K + +   G E+L K MI E++      L+  A+  + + K+ + ++I      
Sbjct: 129 NIHKHVKQISLQHLGSEALKKKMIGEIDRVTYEHLRSKANQGSFDAKEAVESVIMAHLTP 188

Query: 181 RLISYDPKKSSENLRENFVAFIQGLISFPLDIPG-TSFHKCL----------------QQ 240
           ++IS    ++   L +N +A        PL +    S +K                  ++
Sbjct: 189 KIISNLKPETQATLVDNIMALGSEWFQSPLKLTTLISIYKVFIARRYALQVIKDVFTRRK 248

Query: 241 NPRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPH 300
             R+   D+ D +++E +K+     +E A++LIF +L  + E+TS   +LAIKFL ++  
Sbjct: 249 ASREMCGDFLDTMVEEGEKEDVIFNEESAINLIFAILVVAKESTSSVTSLAIKFLAENHK 308

Query: 301 VLNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYK-SM 360
            L EL                            + EH  IL+ R  K  G++W EY+  M
Sbjct: 309 ALAEL----------------------------KREHAAILQNRNGKGAGVSWEEYRHQM 368

Query: 361 TFTFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAV------ 420
           TFT  V             +NETLR+AN+AP ++R+A+ DVE KGYTIP GW V      
Sbjct: 369 TFTNMV-------------INETLRMANMAPIMYRKAVNDVEIKGYTIPAGWIVAVIPPA 428

Query: 421 ----------------MRCDG-----ASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVT 463
                            R +G      SK FM FGGG+R C+GA+FA++Q+++F+H+LVT
Sbjct: 429 VHFNDAIYENPLEFNPWRWEGKELRSGSKTFMVFGGGVRQCVGAEFARLQISIFIHHLVT 473

BLAST of Lsi03G012770 vs. Swiss-Prot
Match: C90A1_ARATH (Cytochrome P450 90A1 OS=Arabidopsis thaliana GN=CYP90A1 PE=2 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 3.0e-52
Identity = 129/386 (33.42%), Postives = 202/386 (52.33%), Query Frame = 1

Query: 29  LPKGSMGFPIIGESRQFFAPNPSFDLSPFIKDRILKYGPIFKTRLVGKPLIISADPELNY 88
           LP GS+G P+IGE+ Q      + +  PFI +R+ +YG +F T L G+P I SADPE N 
Sbjct: 31  LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNR 90

Query: 89  IIFQQEEQLFECWYPETFRKIFGGQFVGSLHGFMHKYLKNMIINVFGIESLK-NMIFEVE 148
            + Q E +LFEC YP +   + G   +  + G +HK + ++ ++      +K +++ +++
Sbjct: 91  FVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDID 150

Query: 149 VAATTRLKKWASHNTVELKDEIANMIFDLTAKRLISYDPKKSSENLRENFVAFIQGLISF 208
                 L  W+S   V L +E   + F+LT K+L+S+DP + SE+LR+ ++  I+G  S 
Sbjct: 151 RLVRFNLDSWSSR--VLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFSL 210

Query: 209 PLDIPGTSFHKCLQQNPRKQHIDYFDYVL---QELQKDGTP---------------LTQE 268
           PL +  T++ K +Q   R++  +    V+   +E +++G                  + E
Sbjct: 211 PLPLFSTTYRKAIQ--ARRKVAEALTVVVMKRREEEEEGAERKKDMLAALLAADDGFSDE 270

Query: 269 IALDLIFLLLFASYETTSIALTLAIKFLIDHPHVLNELTVHSFLDVSLVSSHPLLTYLIS 328
             +D +  LL A YETTS  +TLA+KFL + P  L +L                      
Sbjct: 271 EIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQL---------------------- 330

Query: 329 MQNLDHQAEHERILKERKNKSGGITWNEYKSMTFTFKVNEAFGKLFSCVIFLNETLRLAN 388
                 + EHE+I +  K+ S  + W++YKSM FT            CV+  NETLR+AN
Sbjct: 331 ------KEEHEKI-RAMKSDSYSLEWSDYKSMPFT-----------QCVV--NETLRVAN 370

Query: 389 IAPGIFRRALKDVEFKGYTIPEGWAV 396
           I  G+FRRA+ DVE KGY IP+GW V
Sbjct: 391 IIGGVFRRAMTDVEIKGYKIPKGWKV 370

BLAST of Lsi03G012770 vs. Swiss-Prot
Match: C72B1_PINTA (Abietadienol/abietadienal oxidase OS=Pinus taeda GN=CYP720B1 PE=1 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 2.0e-51
Identity = 140/481 (29.11%), Postives = 227/481 (47.19%), Query Frame = 1

Query: 29  LPKGSMGFPIIGESRQFFAPNPSFDLSPFIKDRILKY-GPIFKTRLVGKPLIISADPELN 88
           LP GS G+P+IGE+  ++    S     FI DR  +Y   +F + L G   +IS+DP+ N
Sbjct: 45  LPPGSTGWPLIGETYSYYRSMTSNRPRQFIDDREKRYDSDVFVSHLFGSQAVISSDPQFN 104

Query: 89  YIIFQQEEQLFECWYPETFRKIFGGQFVGSLHGFMHKYLKNMIINVFGIESLK-NMIFEV 148
             + Q E + F+  YP+  + + G   + S+HG + + L  + +N+   E LK + + E+
Sbjct: 105 KYVLQNEGRFFQAHYPKALKALIGDYGLLSVHGDLQRKLHGIAVNLLRFERLKFDFMEEI 164

Query: 149 EVAATTRLKKWASHNTVELKDEIANMIFDLTAKRLISYDPKKSSENLRENFVAFIQGLIS 208
           +    + L +W     + L++E   M+ +L AK+L+   P K +  + E FV +   +I+
Sbjct: 165 QNLVHSTLDRWVDKKEIALQNECHQMVLNLMAKQLLDLSPSKETNEICELFVDYTNAVIA 224

Query: 209 FPLDIPGTSFHKCLQ---------QNPRKQ-----HIDYFDYVLQELQKDGTPLTQEIAL 268
            P+ IPG+++ K L+          N  K+     HI + D + + L++D   ++ EI  
Sbjct: 225 IPIKIPGSTYAKGLKARELLIRKISNMIKERRDHPHIVHKDLLTKLLEEDS--ISDEIIC 284

Query: 269 DLIFLLLFASYETTSIALTLAIKFLIDHPHVLNELTVHSFLDVSLVSSHPLLTYLISMQN 328
           D I  LLFA +ET+S A+T AIKFL   P  L ++                         
Sbjct: 285 DFILFLLFAGHETSSRAMTFAIKFLTTCPKALTQM------------------------- 344

Query: 329 LDHQAEHERILKERKNKSGGITWNEYKSMTFTFKVNEAFGKLFSCVIFLNETLRLANIAP 388
              + EH+ ILK  K     + W++YKSM FT            CVI  NETLRL N  P
Sbjct: 345 ---KEEHDAILK-AKGGHKKLEWDDYKSMKFT-----------QCVI--NETLRLGNFGP 404

Query: 389 GIFRRALKDVEFKGYTIPEGWAV--------------------------MRCDGASKH-F 448
           G+FR   +D + K   IP+GW V                          +  D ++ H F
Sbjct: 405 GVFRETKEDTKVKDCLIPKGWVVFAFLTATHLDEKFHNEALTFNPWRWELDQDVSNNHLF 464

Query: 449 MAFGGGMRFCIGADFAKMQMAVFLHNLVTNYTLKAIKGGNIVRTPGVQFPDGFHVEIVKK 467
             FGGG R C G+  A++++A+FLH  +T +  +A+   +    P      GF + +  +
Sbjct: 465 SPFGGGARLCPGSHLARLELALFLHIFITRFRWEALADEHPSYFPLPYLAKGFPMRLYNR 481

BLAST of Lsi03G012770 vs. Swiss-Prot
Match: C90B1_ARATH (Cytochrome P450 90B1 OS=Arabidopsis thaliana GN=CYP90B1 PE=1 SV=2)

HSP 1 Score: 204.5 bits (519), Expect = 2.6e-51
Identity = 139/487 (28.54%), Postives = 236/487 (48.46%), Query Frame = 1

Query: 29  LPKGSMGFPIIGESRQFFAPNPSFDLSPFIKDRILKYGPIFKTRLVGKPLIISADPELNY 88
           LP G  G+P +GE+  +  P  +  L  F++  + KYG I+++ L G+P I+SAD  LN 
Sbjct: 39  LPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHVSKYGKIYRSNLFGEPTIVSADAGLNR 98

Query: 89  IIFQQEEQLFECWYPETFRKIFGGQFVGSLHGFMHKYLKNMIINVFGIESLKNMIF-EVE 148
            I Q E +LFEC YP +   I G   +  L G MH+ ++++ +N      L+ ++  +VE
Sbjct: 99  FILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRSISLNFLSHARLRTILLKDVE 158

Query: 149 VAATTRLKKWASHNTVELKDEIANMIFDLTAKRLISYDP-KKSSENLRENFVAFIQGLIS 208
                 L  W  ++    +DE     F+L AK ++S DP ++ +E L++ +V F++G++S
Sbjct: 159 RHTLFVLDSWQQNSIFSAQDEAKKFTFNLMAKHIMSMDPGEEETEQLKKEYVTFMKGVVS 218

Query: 209 FPLDIPGTSFHKCLQQNPRKQHIDYFDYVLQELQ---KDGTPLTQEIALDLIFLLLFASY 268
            PL++PGT++HK LQ   R   + + +  ++E +   K+     +E+  +    +  + +
Sbjct: 219 APLNLPGTAYHKALQS--RATILKFIERKMEERKLDIKEEDQEEEEVKTEDEAEMSKSDH 278

Query: 269 ETTSIALTLAIKFLIDHPHVLNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQA------ 328
                     + +++ H ++  E  +   L + L + H   +  I++     QA      
Sbjct: 279 VRKQRTDDDLLGWVLKHSNLSTEQILDLILSL-LFAGHETSSVAIALAIFFLQACPKAVE 338

Query: 329 ----EHERILKERKN-KSGGITWNEYKSMTFTFKVNEAFGKLFSCVIFLNETLRLANIAP 388
               EH  I + +K      + W++YK M FT            CVI  NETLRL N+  
Sbjct: 339 ELREEHLEIARAKKELGESELNWDDYKKMDFT-----------QCVI--NETLRLGNVVR 398

Query: 389 GIFRRALKDVEFKGYTIPEGWAVM--------------------------RCDGAS---- 448
            + R+ALKDV +KGY IP GW V+                          + +GAS    
Sbjct: 399 FLHRKALKDVRYKGYDIPSGWKVLPVISAVHLDNSRYDQPNLFNPWRWQQQNNGASSSGS 458

Query: 449 -------KHFMAFGGGMRFCIGADFAKMQMAVFLHNLVTNYTLKAIKGGNIVRTPGVQFP 463
                   ++M FGGG R C G++ AK++MAVF+H+LV  +  +  +       P V FP
Sbjct: 459 GSFSTWGNNYMPFGGGPRLCAGSELAKLEMAVFIHHLVLKFNWELAEDDKPFAFPFVDFP 509

BLAST of Lsi03G012770 vs. TrEMBL
Match: A0A0A0LCN3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G845470 PE=3 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 2.4e-197
Identity = 362/511 (70.84%), Postives = 395/511 (77.30%), Query Frame = 1

Query: 1   MWVVMAAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIKD 60
           MW ++ AI  FSV IW+Y LK  KY GKLPKGSMGFP +GES QFFAPNPSFDLSPFIK 
Sbjct: 1   MWFIIGAILSFSVTIWVYALKNPKYNGKLPKGSMGFPFVGESTQFFAPNPSFDLSPFIKH 60

Query: 61  RILKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLHG 120
           RILKYGPIFKTRLVGKPLIISAD ELN+IIFQ+EE+LFECWYPETFRKIFG + VGSLHG
Sbjct: 61  RILKYGPIFKTRLVGKPLIISADAELNHIIFQKEEELFECWYPETFRKIFGVKSVGSLHG 120

Query: 121 FMHKYLKNMIINVFGIESLKNMIFEVEVAATTRLKKWASHN-TVELKDEIANMIFDLTAK 180
           FMHKYLKNMI NVFGIESLKNMI EVEV +TTRLKKWASHN  VELKDEIANMIFDL+AK
Sbjct: 121 FMHKYLKNMITNVFGIESLKNMISEVEVTSTTRLKKWASHNDIVELKDEIANMIFDLSAK 180

Query: 181 RLISYDPKKSSENLRENFVAFIQGLISFPLDIPGTSFHKCL-----------------QQ 240
           RLISYDP+K  EN+RENFVAFIQGLISFP+DIPGTS+HKCL                 QQ
Sbjct: 181 RLISYDPEKCGENMRENFVAFIQGLISFPIDIPGTSYHKCLQGRKRVMRMLENILKERQQ 240

Query: 241 NPRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPH 300
           NPR+Q +DYFD+V+QEL+KDGTPLTQ++ALDLIFLLLFAS+ETTSIALTLAIKFL DHPH
Sbjct: 241 NPREQQVDYFDFVIQELKKDGTPLTQQVALDLIFLLLFASHETTSIALTLAIKFLTDHPH 300

Query: 301 VLNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYKSMT 360
           VLN+LT                            AEHE ILK+RKNKS  I+WNEYKSMT
Sbjct: 301 VLNQLT----------------------------AEHEGILKKRKNKSDKISWNEYKSMT 360

Query: 361 FTFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAVM------ 420
           FTFK             F+NETLRLANIAPGIFRRAL+DVEFKGYTIPEGWAVM      
Sbjct: 361 FTFK-------------FMNETLRLANIAPGIFRRALRDVEFKGYTIPEGWAVMVCPPAI 420

Query: 421 ----------------------RCDGASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVT 466
                                 RCDGASK+FMAFGGG+RFCIGADFAK+QMAVFLH+LVT
Sbjct: 421 HLDPKAFADPLAFNPWRWDEEQRCDGASKNFMAFGGGIRFCIGADFAKLQMAVFLHHLVT 470

BLAST of Lsi03G012770 vs. TrEMBL
Match: A0A0A0L5U2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G038790 PE=3 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 3.0e-160
Identity = 297/510 (58.24%), Postives = 361/510 (70.78%), Query Frame = 1

Query: 1   MWVVM-AAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIK 60
           MW+++  AI   S   W+Y     K  GKLP GSMG P++GE+ QFFAPN S D+ PFI+
Sbjct: 1   MWLLLIGAIVVLSFTHWVYSWINPKCNGKLPPGSMGLPLLGETLQFFAPNTSSDIPPFIR 60

Query: 61  DRILKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLH 120
            R+ +YGPIF+T LVG+PLI+S DP+LN+ IFQQE QLF+ WYP+TF +IFG Q VGSLH
Sbjct: 61  KRMDRYGPIFRTSLVGRPLIVSTDPDLNHFIFQQEGQLFQSWYPDTFTEIFGRQNVGSLH 120

Query: 121 GFMHKYLKNMIINVFGIESLKNMIFEVEVAATTRLKKWASHNTVELKDEIANMIFDLTAK 180
           GFM+KYLKNM++++FG ESLK MI EVE  AT RLK+W+SHN VELKD+ A+MIFDLTAK
Sbjct: 121 GFMYKYLKNMVLHLFGPESLKKMIPEVEAVATRRLKQWSSHNPVELKDKTASMIFDLTAK 180

Query: 181 RLISYDPKKSSENLRENFVAFIQGLISFPLDIPGTSFHKCLQ-----------------Q 240
           +LISYD + SSENLR+NFVAFIQGLISFPL++PGT+++KCLQ                  
Sbjct: 181 KLISYDSENSSENLRDNFVAFIQGLISFPLNVPGTAYNKCLQGRKKAMRMLKNMLQERRT 240

Query: 241 NPRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPH 300
           NPRKQ ID+FD+VL+EL+KDGT LT+EIALDL+F+LLFAS+ETTS+A+T AIKFL+++PH
Sbjct: 241 NPRKQRIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300

Query: 301 VLNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYKSMT 360
           VL ELT                            AEHE ILK R+     +TW EYKSMT
Sbjct: 301 VLEELT----------------------------AEHEGILKRREISDSRLTWGEYKSMT 360

Query: 361 FTFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAVMRC---- 420
           FTF+             F+NET+RLANI PGIFR+ALKD+EFKGYTIP GWAVM C    
Sbjct: 361 FTFQ-------------FINETVRLANIVPGIFRKALKDIEFKGYTIPSGWAVMVCPPAV 420

Query: 421 -----------------------DGASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVTN 466
                                  +G SKHFMAFGGGMRFC+G DF K+QMAVFLH LVT 
Sbjct: 421 HLNPEKYVDPLAFNPWRWEKSELNGGSKHFMAFGGGMRFCVGTDFTKVQMAVFLHCLVTK 469

BLAST of Lsi03G012770 vs. TrEMBL
Match: W9SLD3_9ROSA (Cytochrome P450 87A3 OS=Morus notabilis GN=L484_010567 PE=3 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 1.2e-153
Identity = 286/508 (56.30%), Postives = 353/508 (69.49%), Query Frame = 1

Query: 1   MWVV-MAAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIK 60
           MW++ + A+   S+  W+Y     K  GKLP GSMGFP++GE+ QFF+PN SFD+ PFIK
Sbjct: 1   MWLLFIGALAVISITHWVYTWMNPKCSGKLPPGSMGFPLLGETLQFFSPNTSFDIPPFIK 60

Query: 61  DRILKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLH 120
            RI KYG IF+T LVG+P++IS D +LNY IFQQE +LF+ WYPETF +IFG Q VGSLH
Sbjct: 61  QRIKKYGAIFRTSLVGRPVVISTDSDLNYFIFQQEGRLFQSWYPETFTEIFGKQNVGSLH 120

Query: 121 GFMHKYLKNMIINVFGIESLKNMIFEVEVAATTRLKKWASHNTVELKDEIANMIFDLTAK 180
           GFM+KYLKNM++N+FG ESLK M+ EVE AA  +L++W++   +ELK+  A MIFDLTAK
Sbjct: 121 GFMYKYLKNMVLNLFGPESLKKMLPEVEQAANGKLRQWSNRGVIELKEATARMIFDLTAK 180

Query: 181 RLISYDPKKSSENLRENFVAFIQGLISFPLDIPGTSFHKCLQ-----------------Q 240
           +LISYD +KSSENLRENFVAFIQGLISFPL+IPGT+++KCL+                 +
Sbjct: 181 KLISYDQEKSSENLRENFVAFIQGLISFPLNIPGTAYNKCLEGRKRAMRMLKKLLQERRE 240

Query: 241 NPRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPH 300
           NP+KQ  D+FDYVL+ELQK+GT LT+ IALDL+F+LLFAS+ETTS+ALTLA+KFL DHP 
Sbjct: 241 NPKKQQSDFFDYVLEELQKEGTILTEAIALDLMFVLLFASFETTSLALTLAVKFLTDHPL 300

Query: 301 VLNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYKSMT 360
           VL +LT                             EHE ILK R+N   GITW EYKSMT
Sbjct: 301 VLKKLT----------------------------EEHEMILKNRENADSGITWKEYKSMT 360

Query: 361 FTFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAVMRC---- 420
           FT +V             +NET+RLANI PGIFR+AL+D++FKGYTIP GWAVM C    
Sbjct: 361 FTIQV-------------INETVRLANIVPGIFRKALRDIQFKGYTIPAGWAVMVCPPAV 420

Query: 421 -----------------------DGASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVTN 464
                                  +GA+KHFMAFGGGMRFC+G +F K+QMAVFLH LVT 
Sbjct: 421 HLNPAKYEDPLAFNPWRWEGVELNGATKHFMAFGGGMRFCVGTEFTKVQMAVFLHCLVTK 467

BLAST of Lsi03G012770 vs. TrEMBL
Match: A0A067FJB9_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g040767mg PE=3 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 1.6e-153
Identity = 285/510 (55.88%), Postives = 355/510 (69.61%), Query Frame = 1

Query: 1   MWVV-MAAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIK 60
           MW + + A+   S+  WLY  +  +  GKLP GSMG P++GE+ QFFAPN S D+ PFIK
Sbjct: 1   MWALCIGALVFVSITHWLYRWRNPRCNGKLPPGSMGLPLLGETLQFFAPNTSSDIPPFIK 60

Query: 61  DRILKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLH 120
           +R+  YGPIF+T LVG+P+IIS DP+LNY IFQQE QLF+ WYP+TF +IFG Q VGSLH
Sbjct: 61  NRMKSYGPIFRTSLVGRPVIISTDPDLNYFIFQQEGQLFQSWYPDTFTEIFGRQNVGSLH 120

Query: 121 GFMHKYLKNMIINVFGIESLKNMIFEVEVAATTRLKKWASHNTVELKDEIANMIFDLTAK 180
           GFM++YLKNM++N+FG ESL+ M+ EVE  A  RL++W+S +TVELK+  A MIFDLTAK
Sbjct: 121 GFMYRYLKNMVLNLFGPESLRKMLPEVEQVARARLQQWSSQDTVELKEATATMIFDLTAK 180

Query: 181 RLISYDPKKSSENLRENFVAFIQGLISFPLDIPGTSFHKCLQ-----------------Q 240
           +LISYD +KSSENLRENFVAF++GLISFPLDIPGT++HKCLQ                  
Sbjct: 181 KLISYDQEKSSENLRENFVAFMEGLISFPLDIPGTAYHKCLQGRERAMKKLKNLLQERRA 240

Query: 241 NPRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPH 300
           NPRK+  D++DYVL+ELQK+GT LT+ IALDL+F+LLFAS+ETTS+A+TLAIKFL ++P 
Sbjct: 241 NPRKEQSDFYDYVLKELQKEGTILTEAIALDLMFVLLFASFETTSVAITLAIKFLSENPL 300

Query: 301 VLNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYKSMT 360
           VL +LT                             EHE ILK R+N   G+TW EYKSMT
Sbjct: 301 VLKQLT----------------------------EEHEAILKNRENVHSGLTWREYKSMT 360

Query: 361 FTFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAVMRC---- 420
           +TF+             F+NET RLANI PGIFR+AL+D++FKGYTIP GWAVM C    
Sbjct: 361 YTFQ-------------FINETARLANIVPGIFRKALRDIQFKGYTIPAGWAVMVCPPAV 420

Query: 421 -----------------------DGASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVTN 466
                                  + ASK+FMAFGGGMRFC+G DF K+QMAVFLH  +T 
Sbjct: 421 HLNPSKYEDPLAFNPSRWKGMETNCASKNFMAFGGGMRFCVGTDFTKVQMAVFLHCFLTK 469

BLAST of Lsi03G012770 vs. TrEMBL
Match: V4TQ27_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10023269mg PE=3 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 4.7e-153
Identity = 284/510 (55.69%), Postives = 354/510 (69.41%), Query Frame = 1

Query: 1   MWVV-MAAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIK 60
           MW + + A+   S+  WLY  +  +  GKLP GSMG P++GE+ QFFAPN S D+ PFIK
Sbjct: 1   MWALCIGALVFVSITHWLYRWRNPRCNGKLPPGSMGLPLLGETLQFFAPNTSSDIPPFIK 60

Query: 61  DRILKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLH 120
           +R+  YGPIF+T LVG+P+IIS DP+LNY IFQQE Q F+ WYP+TF +IFG Q VGSLH
Sbjct: 61  NRMKSYGPIFRTSLVGRPVIISTDPDLNYFIFQQEGQFFQSWYPDTFTEIFGRQNVGSLH 120

Query: 121 GFMHKYLKNMIINVFGIESLKNMIFEVEVAATTRLKKWASHNTVELKDEIANMIFDLTAK 180
           GFM++YLKNM++N+FG ESL+ M+ EVE  A  RL++W+S +TVELK+  A MIFDLTAK
Sbjct: 121 GFMYRYLKNMVLNLFGPESLRKMLPEVEQVARARLQQWSSQDTVELKEATATMIFDLTAK 180

Query: 181 RLISYDPKKSSENLRENFVAFIQGLISFPLDIPGTSFHKCLQ-----------------Q 240
           +LISYD +KSSENLRENFVAF++GLISFPLDIPGT++HKCLQ                  
Sbjct: 181 KLISYDQEKSSENLRENFVAFMEGLISFPLDIPGTAYHKCLQGRERAMKKLKNLLQERRA 240

Query: 241 NPRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPH 300
           NPRK+  D++DYVL+ELQK+GT LT+ IALDL+F+LLFAS+ETTS+A+TLAIKFL ++P 
Sbjct: 241 NPRKEQSDFYDYVLKELQKEGTILTEAIALDLMFVLLFASFETTSVAITLAIKFLSENPL 300

Query: 301 VLNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYKSMT 360
           VL +LT                             EHE ILK R+N   G+TW EYKSMT
Sbjct: 301 VLKQLT----------------------------EEHEAILKNRENVHSGLTWREYKSMT 360

Query: 361 FTFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAVMRC---- 420
           +TF+             F+NET RLANI PGIFR+AL+D++FKGYTIP GWAVM C    
Sbjct: 361 YTFQ-------------FINETARLANIVPGIFRKALRDIQFKGYTIPAGWAVMVCPPAV 420

Query: 421 -----------------------DGASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVTN 466
                                  + ASK+FMAFGGGMRFC+G DF K+QMAVFLH  +T 
Sbjct: 421 HLNPAKYEDPLAFNPSRWKGMETNCASKNFMAFGGGMRFCVGTDFTKVQMAVFLHCFLTK 469

BLAST of Lsi03G012770 vs. TAIR10
Match: AT1G12740.2 (AT1G12740.2 cytochrome P450, family 87, subfamily A, polypeptide 2)

HSP 1 Score: 522.7 bits (1345), Expect = 2.4e-148
Identity = 272/518 (52.51%), Postives = 347/518 (66.99%), Query Frame = 1

Query: 1   MWVVM--AAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFI 60
           MW ++   ++   S+  W+Y  +  K  GKLP GSMGFP++GES QFF PN + D+ PFI
Sbjct: 1   MWALLIWVSLLLISITHWVYSWRNPKCRGKLPPGSMGFPLLGESIQFFKPNKTSDIPPFI 60

Query: 61  KDRI------LKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGG 120
           K+R+       +YGPIFKT LVG+P+I+S D +L+Y +F QE + F+ WYP+TF  IFG 
Sbjct: 61  KERVKNDVDMCRYGPIFKTNLVGRPVIVSTDADLSYFVFNQEGRCFQSWYPDTFTHIFGK 120

Query: 121 QFVGSLHGFMHKYLKNMIINVFGIESLKNMIFEVEVAATTRLKKWASHNTVELKDEIANM 180
           + VGSLHGFM+KYLKNM++ +FG + LK M+ +VE+ A  RL+ W++ ++VELKD  A+M
Sbjct: 121 KNVGSLHGFMYKYLKNMVLTLFGHDGLKKMLPQVEMTANKRLELWSNQDSVELKDATASM 180

Query: 181 IFDLTAKRLISYDPKKSSENLRENFVAFIQGLISFPLDIPGTSFHKCLQ----------- 240
           IFDLTAK+LIS+DP KSSENLR NFVAFIQGLISFP DIPGT++HKCLQ           
Sbjct: 181 IFDLTAKKLISHDPDKSSENLRANFVAFIQGLISFPFDIPGTAYHKCLQGRAKAMKMLRN 240

Query: 241 ------QNPRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIK 300
                 +NPRK   D+FDYV++E+QK+GT LT+EIALDL+F+LLFAS+ETTS+ALTLAIK
Sbjct: 241 MLQERRENPRKNPSDFFDYVIEEIQKEGTILTEEIALDLMFVLLFASFETTSLALTLAIK 300

Query: 301 FLIDHPHVLNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITW 360
           FL D P VL  LT                             EHE IL+ R++   G+TW
Sbjct: 301 FLSDDPEVLKRLT----------------------------EEHETILRNREDADSGLTW 360

Query: 361 NEYKSMTFTFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAV 420
            EYKSMT+TF+             F+NET RLANI P IFR+AL+D++FK YTIP GWAV
Sbjct: 361 EEYKSMTYTFQ-------------FINETARLANIVPAIFRKALRDIKFKDYTIPAGWAV 420

Query: 421 M----------------------RCDG-----ASKHFMAFGGGMRFCIGADFAKMQMAVF 467
           M                      R +G     ASKHFMAFGGGMRFC+G DF K+QMA F
Sbjct: 421 MVCPPAVHLNPEMYKDPLVFNPSRWEGSKVTNASKHFMAFGGGMRFCVGTDFTKLQMAAF 477

BLAST of Lsi03G012770 vs. TAIR10
Match: AT1G78490.1 (AT1G78490.1 cytochrome P450, family 708, subfamily A, polypeptide 3)

HSP 1 Score: 263.8 bits (673), Expect = 2.0e-70
Identity = 168/506 (33.20%), Postives = 254/506 (50.20%), Query Frame = 1

Query: 3   VVMAAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIKDRI 62
           ++M A+    +  WLY     K  GKLP GSMGFPIIGE+  FF P     +  F+K R+
Sbjct: 9   MLMVALVVVRISHWLYRWSNPKCPGKLPPGSMGFPIIGETLDFFKPCGVEGIPTFVKKRM 68

Query: 63  LKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLHGFM 122
           ++YGP+F+T + G   ++S DP++ + IF+QE   FE  YP+ F K+FG   +     F+
Sbjct: 69  IRYGPLFRTNIFGSKTVVSTDPDVIHQIFRQENTSFELGYPDIFVKVFGKDNLFLKEVFI 128

Query: 123 HKYLKNMIINVFGIESLK-NMIFEVEVAATTRLKKWASHNTVELKDEIANMIFDLTAKRL 182
           HKYL+ + + + G E LK  M+  ++ A    ++  AS  +  ++ E+ N++      +L
Sbjct: 129 HKYLQKITMQILGSEGLKQTMLGNMDKATRDHIRSIASQGSFNVRKEVENLVVAYMTPKL 188

Query: 183 ISYDPKKSSENLRENFVAF-----------------IQGLISFPLDIPGTSFHKCLQQNP 242
           IS    ++   L +N  AF                  + L S    I        +++  
Sbjct: 189 ISNLKPETQSKLIDNLNAFNLDWFKSFLRLSTWKAVTKALKSREEAIQVMKDVLMMRKET 248

Query: 243 RKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPHVL 302
           R++  D+ + +L+EL+KDG+   Q  A++LIFLL FA  E TS    LA+KF+   P VL
Sbjct: 249 REKQEDFLNTLLEELEKDGSFFDQGSAINLIFLLAFALREGTSSCTALAVKFISKDPKVL 308

Query: 303 NELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYK-SMTF 362
            EL                            + EH+ I+  RK+K  G++W EY+ +MTF
Sbjct: 309 AEL----------------------------KREHKAIVDNRKDKEAGVSWEEYRHNMTF 368

Query: 363 TFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAV-------- 422
           T  V+             NE LRLAN  P +FR+A++DVE KGYTIP GW V        
Sbjct: 369 TNMVS-------------NEVLRLANTTPLLFRKAVQDVEIKGYTIPAGWIVAVAPSAVH 428

Query: 423 --------------MRCDG-----ASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVTNY 463
                          R +G      SK FMAFG G+R C+GA+F+++QMA+FLH+LV  Y
Sbjct: 429 FDPAIYENPFEFNPWRWEGKEMIWGSKTFMAFGYGVRLCVGAEFSRLQMAIFLHHLVAYY 473

BLAST of Lsi03G012770 vs. TAIR10
Match: AT5G48000.1 (AT5G48000.1 cytochrome P450, family 708, subfamily A, polypeptide 2)

HSP 1 Score: 259.2 bits (661), Expect = 4.9e-69
Identity = 165/508 (32.48%), Postives = 256/508 (50.39%), Query Frame = 1

Query: 1   MWVVMAAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIKD 60
           +WV+  A    S   WLY     K  GKLP GSMG PIIGE+  FF P+  +++SPF+K 
Sbjct: 50  VWVIAVAAVVISK--WLYRWSNPKCNGKLPPGSMGLPIIGETCDFFEPHGLYEISPFVKK 109

Query: 61  RILKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLHG 120
           R+LKYGP+F+T + G   ++  +P++ + +F+QE + F   YPE F K FG + V   HG
Sbjct: 110 RMLKYGPLFRTNIFGSNTVVLTEPDIIFEVFRQENKSFVFSYPEAFVKPFGKENVFLKHG 169

Query: 121 FMHKYLKNMIINVFGIESL-KNMIFEVEVAATTRLKKWASHNTVELKDEIANMIFDLTAK 180
            +HK++K + +   G E+L K MI E++      L+  A+  + + K+ + ++I      
Sbjct: 170 NIHKHVKQISLQHLGSEALKKKMIGEIDRVTYEHLRSKANQGSFDAKEAVESVIMAHLTP 229

Query: 181 RLISYDPKKSSENLRENFVAFIQGLISFPLDIPG-TSFHKCL----------------QQ 240
           ++IS    ++   L +N +A        PL +    S +K                  ++
Sbjct: 230 KIISNLKPETQATLVDNIMALGSEWFQSPLKLTTLISIYKVFIARRYALQVIKDVFTRRK 289

Query: 241 NPRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPH 300
             R+   D+ D +++E +K+     +E A++LIF +L  + E+TS   +LAIKFL ++  
Sbjct: 290 ASREMCGDFLDTMVEEGEKEDVIFNEESAINLIFAILVVAKESTSSVTSLAIKFLAENHK 349

Query: 301 VLNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYK-SM 360
            L EL                            + EH  IL+ R  K  G++W EY+  M
Sbjct: 350 ALAEL----------------------------KREHAAILQNRNGKGAGVSWEEYRHQM 409

Query: 361 TFTFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAV------ 420
           TFT  V             +NETLR+AN+AP ++R+A+ DVE KGYTIP GW V      
Sbjct: 410 TFTNMV-------------INETLRMANMAPIMYRKAVNDVEIKGYTIPAGWIVAVIPPA 469

Query: 421 ----------------MRCDG-----ASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVT 463
                            R +G      SK FM FGGG+R C+GA+FA++Q+++F+H+LVT
Sbjct: 470 VHFNDAIYENPLEFNPWRWEGKELRSGSKTFMVFGGGVRQCVGAEFARLQISIFIHHLVT 514

BLAST of Lsi03G012770 vs. TAIR10
Match: AT1G55940.1 (AT1G55940.1 cytochrome P450, family 708, subfamily A, polypeptide 1)

HSP 1 Score: 256.9 bits (655), Expect = 2.4e-68
Identity = 171/534 (32.02%), Postives = 260/534 (48.69%), Query Frame = 1

Query: 1   MWVV----MAAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSP 60
           MW V    + A+    + +WLY        GKLP GSMGFP+IGE+ +FF P    ++ P
Sbjct: 148 MWGVALSFVIALVVVKISLWLYRWANPNCSGKLPPGSMGFPVIGETVEFFKPYSFNEIHP 207

Query: 61  FIKDRILKYG-PIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFV 120
           F+K R+ K+G  +F+T ++G   I+S DPE+N+ I +QE + F   YPE   +IFG   +
Sbjct: 208 FVKKRMFKHGGSLFRTNILGSKTIVSTDPEVNFEILKQENRCFIMSYPEALVRIFGKDNL 267

Query: 121 GSLHGF-MHKYLKNMIINVFGIESLKN-MIFEVEVAATTRLKKWASHNTVELKDEIANMI 180
               G   H+Y++++ + + G E LK   I ++++A +  LK  +    V++KD    +I
Sbjct: 268 FFKQGKDFHRYMRHIALQLLGPECLKQRFIQQIDIATSEHLKSVSFQGVVDVKDTSGRLI 327

Query: 181 FDLTAKRLISYDPKKSSENLRENFVAFIQGLISFPLD-------------------IPGT 240
            +     +IS    ++   L E+F  F   L+  P D                   I   
Sbjct: 328 LEQMILMIISNIKPETKSKLIESFRDFSFDLVRSPFDPSFWNALYNGLMDFVWLLNILDP 387

Query: 241 SFHKCLQQNPRK------------------QHIDYFDYVLQELQKDGTPLTQEIALDLIF 300
            F+   + N  K                  ++ D+ + ++ E++K+G  + +E +++LI 
Sbjct: 388 LFYVKARSNVMKMLKRMFKERREEATSDDSKYGDFMETMIYEVEKEGDTINEERSVELIL 447

Query: 301 LLLFASYETTSIALTLAIKFLIDHPHVLNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQ 360
            LL ASYETTS    L +KF+ ++P VL EL                            +
Sbjct: 448 SLLIASYETTSTMTALTVKFIAENPKVLMEL----------------------------K 507

Query: 361 AEHERILKERKNKSGGITWNEYKSM-TFTFKVNEAFGKLFSCVIFLNETLRLANIAPGIF 420
            EHE IL+ R +K  G+TW EY+SM  FT  V             +NE+LRL +++P +F
Sbjct: 508 REHETILQNRADKESGVTWKEYRSMMNFTHMV-------------INESLRLGSLSPAMF 567

Query: 421 RRALKDVEFKGYTIPEGWAVM----------------------RCDG-----ASKHFMAF 463
           R+A+ DVE KGYTIP GW V+                      R +G      SK FMAF
Sbjct: 568 RKAVNDVEIKGYTIPAGWIVLVVPSLLHYDPQIYEQPCEFNPWRWEGKELLSGSKTFMAF 627

BLAST of Lsi03G012770 vs. TAIR10
Match: AT3G44970.1 (AT3G44970.1 Cytochrome P450 superfamily protein)

HSP 1 Score: 250.4 bits (638), Expect = 2.3e-66
Identity = 162/509 (31.83%), Postives = 253/509 (49.71%), Query Frame = 1

Query: 10  GFSVII--------WLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIKDR 69
           GF VI+        W Y     K  GKLP GSMGFPIIGE+  FF P   +++SP++K +
Sbjct: 8   GFCVIVLVVARVGHWWYQWSNPKSNGKLPPGSMGFPIIGETLDFFKPYGFYEISPYLKKK 67

Query: 70  ILKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLHGF 129
           +L+YGP+F+T ++G   ++S D ++N  I +QE + F   YP+   K  G   +    G 
Sbjct: 68  MLRYGPLFRTNILGVKTVVSTDKDVNMEILRQENKSFILSYPDGLMKPLGKDSLFLKIGN 127

Query: 130 MHKYLKNMIINVFGIESLKNMIF-EVEVAATTRLKKWASHNTVELKDEIANMIFDLTAKR 189
           +HK++K + +++   E LK  I  +++      L   A    +++KD ++ +I      +
Sbjct: 128 IHKHIKQITLHLLSSEGLKRKILKDMDRVTREHLSSKAKTGRLDVKDAVSKLIIAHLTPK 187

Query: 190 LISYDPKKSSENLRENFVAF------IQGLISFPLDIPGTSFH-----------KCLQQN 249
           ++S    ++   L   F AF         LIS    +  T +              +++ 
Sbjct: 188 MMSNLKPQTQAKLMGIFKAFTFDWFRTSYLISAGKGLYNTLWACREGMREIKDIYTMRKT 247

Query: 250 PRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPHV 309
             +++ D+ +  ++E +K G  L +   + LIF L   + +TTS A+ LA+KFL+++P V
Sbjct: 248 SEEKYDDFLNTAIEESEKAGELLNENAIITLIFTLSCVTQDTTSKAICLAVKFLLENPKV 307

Query: 310 LNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYK-SMT 369
           L EL                            + EHE IL+ R++K GG+TW EY+  MT
Sbjct: 308 LAEL----------------------------KKEHEVILESREDKEGGVTWEEYRHKMT 367

Query: 370 FTFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAVM------ 429
           FT  V             +NE+LR+ N+AP +FR+A+KDVE KGYTIP GW VM      
Sbjct: 368 FTNMV-------------INESLRITNLAPMLFRKAVKDVEIKGYTIPAGWIVMIIPSVV 427

Query: 430 ----------------RCDG-----ASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVTN 465
                           R +G      SK FM FG G+R C GA+FA++Q++VFLH+LVT 
Sbjct: 428 HFDPEIYENPFEFNPWRWEGKELRAGSKTFMVFGTGLRQCAGAEFARLQISVFLHHLVTT 475

BLAST of Lsi03G012770 vs. NCBI nr
Match: gi|449446791|ref|XP_004141154.1| (PREDICTED: cytochrome P450 87A3-like [Cucumis sativus])

HSP 1 Score: 696.4 bits (1796), Expect = 3.4e-197
Identity = 362/511 (70.84%), Postives = 395/511 (77.30%), Query Frame = 1

Query: 1   MWVVMAAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIKD 60
           MW ++ AI  FSV IW+Y LK  KY GKLPKGSMGFP +GES QFFAPNPSFDLSPFIK 
Sbjct: 1   MWFIIGAILSFSVTIWVYALKNPKYNGKLPKGSMGFPFVGESTQFFAPNPSFDLSPFIKH 60

Query: 61  RILKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLHG 120
           RILKYGPIFKTRLVGKPLIISAD ELN+IIFQ+EE+LFECWYPETFRKIFG + VGSLHG
Sbjct: 61  RILKYGPIFKTRLVGKPLIISADAELNHIIFQKEEELFECWYPETFRKIFGVKSVGSLHG 120

Query: 121 FMHKYLKNMIINVFGIESLKNMIFEVEVAATTRLKKWASHN-TVELKDEIANMIFDLTAK 180
           FMHKYLKNMI NVFGIESLKNMI EVEV +TTRLKKWASHN  VELKDEIANMIFDL+AK
Sbjct: 121 FMHKYLKNMITNVFGIESLKNMISEVEVTSTTRLKKWASHNDIVELKDEIANMIFDLSAK 180

Query: 181 RLISYDPKKSSENLRENFVAFIQGLISFPLDIPGTSFHKCL-----------------QQ 240
           RLISYDP+K  EN+RENFVAFIQGLISFP+DIPGTS+HKCL                 QQ
Sbjct: 181 RLISYDPEKCGENMRENFVAFIQGLISFPIDIPGTSYHKCLQGRKRVMRMLENILKERQQ 240

Query: 241 NPRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPH 300
           NPR+Q +DYFD+V+QEL+KDGTPLTQ++ALDLIFLLLFAS+ETTSIALTLAIKFL DHPH
Sbjct: 241 NPREQQVDYFDFVIQELKKDGTPLTQQVALDLIFLLLFASHETTSIALTLAIKFLTDHPH 300

Query: 301 VLNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYKSMT 360
           VLN+LT                            AEHE ILK+RKNKS  I+WNEYKSMT
Sbjct: 301 VLNQLT----------------------------AEHEGILKKRKNKSDKISWNEYKSMT 360

Query: 361 FTFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAVM------ 420
           FTFK             F+NETLRLANIAPGIFRRAL+DVEFKGYTIPEGWAVM      
Sbjct: 361 FTFK-------------FMNETLRLANIAPGIFRRALRDVEFKGYTIPEGWAVMVCPPAI 420

Query: 421 ----------------------RCDGASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVT 466
                                 RCDGASK+FMAFGGG+RFCIGADFAK+QMAVFLH+LVT
Sbjct: 421 HLDPKAFADPLAFNPWRWDEEQRCDGASKNFMAFGGGIRFCIGADFAKLQMAVFLHHLVT 470

BLAST of Lsi03G012770 vs. NCBI nr
Match: gi|659096178|ref|XP_008448963.1| (PREDICTED: cytochrome P450 87A3 isoform X1 [Cucumis melo])

HSP 1 Score: 580.9 bits (1496), Expect = 2.1e-162
Identity = 300/510 (58.82%), Postives = 364/510 (71.37%), Query Frame = 1

Query: 1   MWVVM-AAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIK 60
           MW+++  AI   S   W+Y  +  K  GKLP GSMG P++GE+ QFFAPN S D+ PFI+
Sbjct: 1   MWLLLIGAIVVLSFTHWVYSWRNPKCNGKLPPGSMGLPLLGETLQFFAPNTSSDIPPFIR 60

Query: 61  DRILKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLH 120
            R+ +YGPIF+T LVG+PLI+S DP+LNY IFQQE QLF+ WYP+TF +IFG Q VGSLH
Sbjct: 61  KRMDRYGPIFRTSLVGRPLIVSTDPDLNYFIFQQEGQLFQSWYPDTFTEIFGRQNVGSLH 120

Query: 121 GFMHKYLKNMIINVFGIESLKNMIFEVEVAATTRLKKWASHNTVELKDEIANMIFDLTAK 180
           GFM+KYLKNM++++FG ESLK MI EVE  AT RLK+W+SHN VELKD+ A+MIFDLTAK
Sbjct: 121 GFMYKYLKNMVLHLFGPESLKKMIPEVEAVATRRLKQWSSHNPVELKDKTASMIFDLTAK 180

Query: 181 RLISYDPKKSSENLRENFVAFIQGLISFPLDIPGTSFHKCLQ-----------------Q 240
           +LISYD + SSENLR+NFVAFIQGLISFPL++PGT+++KCLQ                  
Sbjct: 181 KLISYDSESSSENLRDNFVAFIQGLISFPLNVPGTAYNKCLQGRKKAMRMLKNMLQERRA 240

Query: 241 NPRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPH 300
           NPRKQ ID+FD+VL+EL+KDGT LT+EIALDL+F+LLFAS+ETTS+A+T AIKFL+++PH
Sbjct: 241 NPRKQRIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300

Query: 301 VLNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYKSMT 360
           VL ELT                            AEHE ILK R+    G+TW EYKSMT
Sbjct: 301 VLEELT----------------------------AEHEGILKRREISDSGLTWGEYKSMT 360

Query: 361 FTFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAVMRC---- 420
           FTF+             F+NET+RLANI PGIFR+ALKD++FKGYTIP GWAVM C    
Sbjct: 361 FTFQ-------------FINETVRLANIVPGIFRKALKDIQFKGYTIPSGWAVMVCPPAV 420

Query: 421 -----------------------DGASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVTN 466
                                  +GASKHFMAFGGGMRFC+G DF K+QMAVFLH LVT 
Sbjct: 421 HLNPEKYVDPLAFNPWRWEKSELNGASKHFMAFGGGMRFCVGTDFTKVQMAVFLHCLVTK 469

BLAST of Lsi03G012770 vs. NCBI nr
Match: gi|449460477|ref|XP_004147972.1| (PREDICTED: cytochrome P450 87A3 isoform X1 [Cucumis sativus])

HSP 1 Score: 573.2 bits (1476), Expect = 4.4e-160
Identity = 297/510 (58.24%), Postives = 361/510 (70.78%), Query Frame = 1

Query: 1   MWVVM-AAIFGFSVIIWLYGLKYSKYYGKLPKGSMGFPIIGESRQFFAPNPSFDLSPFIK 60
           MW+++  AI   S   W+Y     K  GKLP GSMG P++GE+ QFFAPN S D+ PFI+
Sbjct: 1   MWLLLIGAIVVLSFTHWVYSWINPKCNGKLPPGSMGLPLLGETLQFFAPNTSSDIPPFIR 60

Query: 61  DRILKYGPIFKTRLVGKPLIISADPELNYIIFQQEEQLFECWYPETFRKIFGGQFVGSLH 120
            R+ +YGPIF+T LVG+PLI+S DP+LN+ IFQQE QLF+ WYP+TF +IFG Q VGSLH
Sbjct: 61  KRMDRYGPIFRTSLVGRPLIVSTDPDLNHFIFQQEGQLFQSWYPDTFTEIFGRQNVGSLH 120

Query: 121 GFMHKYLKNMIINVFGIESLKNMIFEVEVAATTRLKKWASHNTVELKDEIANMIFDLTAK 180
           GFM+KYLKNM++++FG ESLK MI EVE  AT RLK+W+SHN VELKD+ A+MIFDLTAK
Sbjct: 121 GFMYKYLKNMVLHLFGPESLKKMIPEVEAVATRRLKQWSSHNPVELKDKTASMIFDLTAK 180

Query: 181 RLISYDPKKSSENLRENFVAFIQGLISFPLDIPGTSFHKCLQ-----------------Q 240
           +LISYD + SSENLR+NFVAFIQGLISFPL++PGT+++KCLQ                  
Sbjct: 181 KLISYDSENSSENLRDNFVAFIQGLISFPLNVPGTAYNKCLQGRKKAMRMLKNMLQERRT 240

Query: 241 NPRKQHIDYFDYVLQELQKDGTPLTQEIALDLIFLLLFASYETTSIALTLAIKFLIDHPH 300
           NPRKQ ID+FD+VL+EL+KDGT LT+EIALDL+F+LLFAS+ETTS+A+T AIKFL+++PH
Sbjct: 241 NPRKQRIDFFDFVLEELEKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300

Query: 301 VLNELTVHSFLDVSLVSSHPLLTYLISMQNLDHQAEHERILKERKNKSGGITWNEYKSMT 360
           VL ELT                            AEHE ILK R+     +TW EYKSMT
Sbjct: 301 VLEELT----------------------------AEHEGILKRREISDSRLTWGEYKSMT 360

Query: 361 FTFKVNEAFGKLFSCVIFLNETLRLANIAPGIFRRALKDVEFKGYTIPEGWAVMRC---- 420
           FTF+             F+NET+RLANI PGIFR+ALKD+EFKGYTIP GWAVM C    
Sbjct: 361 FTFQ-------------FINETVRLANIVPGIFRKALKDIEFKGYTIPSGWAVMVCPPAV 420

Query: 421 -----------------------DGASKHFMAFGGGMRFCIGADFAKMQMAVFLHNLVTN 466
                                  +G SKHFMAFGGGMRFC+G DF K+QMAVFLH LVT 
Sbjct: 421 HLNPEKYVDPLAFNPWRWEKSELNGGSKHFMAFGGGMRFCVGTDFTKVQMAVFLHCLVTK 469

BLAST of Lsi03G012770 vs. NCBI nr
Match: gi|659096180|ref|XP_008448964.1| (PREDICTED: cytochrome P450 87A3 isoform X2 [Cucumis melo])

HSP 1 Score: 566.6 bits (1459), Expect = 4.1e-158
Identity = 289/481 (60.08%), Postives = 349/481 (72.56%), Query Frame = 1

Query: 29  LPKGSMGFPIIGESRQFFAPNPSFDLSPFIKDRILKYGPIFKTRLVGKPLIISADPELNY 88
           L +GSMG P++GE+ QFFAPN S D+ PFI+ R+ +YGPIF+T LVG+PLI+S DP+LNY
Sbjct: 10  LEEGSMGLPLLGETLQFFAPNTSSDIPPFIRKRMDRYGPIFRTSLVGRPLIVSTDPDLNY 69

Query: 89  IIFQQEEQLFECWYPETFRKIFGGQFVGSLHGFMHKYLKNMIINVFGIESLKNMIFEVEV 148
            IFQQE QLF+ WYP+TF +IFG Q VGSLHGFM+KYLKNM++++FG ESLK MI EVE 
Sbjct: 70  FIFQQEGQLFQSWYPDTFTEIFGRQNVGSLHGFMYKYLKNMVLHLFGPESLKKMIPEVEA 129

Query: 149 AATTRLKKWASHNTVELKDEIANMIFDLTAKRLISYDPKKSSENLRENFVAFIQGLISFP 208
            AT RLK+W+SHN VELKD+ A+MIFDLTAK+LISYD + SSENLR+NFVAFIQGLISFP
Sbjct: 130 VATRRLKQWSSHNPVELKDKTASMIFDLTAKKLISYDSESSSENLRDNFVAFIQGLISFP 189

Query: 209 LDIPGTSFHKCLQ-----------------QNPRKQHIDYFDYVLQELQKDGTPLTQEIA 268
           L++PGT+++KCLQ                  NPRKQ ID+FD+VL+EL+KDGT LT+EIA
Sbjct: 190 LNVPGTAYNKCLQGRKKAMRMLKNMLQERRANPRKQRIDFFDFVLEELEKDGTLLTEEIA 249

Query: 269 LDLIFLLLFASYETTSIALTLAIKFLIDHPHVLNELTVHSFLDVSLVSSHPLLTYLISMQ 328
           LDL+F+LLFAS+ETTS+A+T AIKFL+++PHVL ELT                       
Sbjct: 250 LDLMFVLLFASFETTSLAITAAIKFLLNNPHVLEELT----------------------- 309

Query: 329 NLDHQAEHERILKERKNKSGGITWNEYKSMTFTFKVNEAFGKLFSCVIFLNETLRLANIA 388
                AEHE ILK R+    G+TW EYKSMTFTF+             F+NET+RLANI 
Sbjct: 310 -----AEHEGILKRREISDSGLTWGEYKSMTFTFQ-------------FINETVRLANIV 369

Query: 389 PGIFRRALKDVEFKGYTIPEGWAVMRC---------------------------DGASKH 448
           PGIFR+ALKD++FKGYTIP GWAVM C                           +GASKH
Sbjct: 370 PGIFRKALKDIQFKGYTIPSGWAVMVCPPAVHLNPEKYVDPLAFNPWRWEKSELNGASKH 429

Query: 449 FMAFGGGMRFCIGADFAKMQMAVFLHNLVTNYTLKAIKGGNIVRTPGVQFPDGFHVEIVK 466
           FMAFGGGMRFC+G DF K+QMAVFLH LVT Y  KAIKGGNIVRTPG+QFPDGFHV+I++
Sbjct: 430 FMAFGGGMRFCVGTDFTKVQMAVFLHCLVTKYRFKAIKGGNIVRTPGLQFPDGFHVQIME 449

BLAST of Lsi03G012770 vs. NCBI nr
Match: gi|778675642|ref|XP_011650442.1| (PREDICTED: cytochrome P450 87A3 isoform X2 [Cucumis sativus])

HSP 1 Score: 560.8 bits (1444), Expect = 2.2e-156
Identity = 286/481 (59.46%), Postives = 347/481 (72.14%), Query Frame = 1

Query: 29  LPKGSMGFPIIGESRQFFAPNPSFDLSPFIKDRILKYGPIFKTRLVGKPLIISADPELNY 88
           L +GSMG P++GE+ QFFAPN S D+ PFI+ R+ +YGPIF+T LVG+PLI+S DP+LN+
Sbjct: 10  LEEGSMGLPLLGETLQFFAPNTSSDIPPFIRKRMDRYGPIFRTSLVGRPLIVSTDPDLNH 69

Query: 89  IIFQQEEQLFECWYPETFRKIFGGQFVGSLHGFMHKYLKNMIINVFGIESLKNMIFEVEV 148
            IFQQE QLF+ WYP+TF +IFG Q VGSLHGFM+KYLKNM++++FG ESLK MI EVE 
Sbjct: 70  FIFQQEGQLFQSWYPDTFTEIFGRQNVGSLHGFMYKYLKNMVLHLFGPESLKKMIPEVEA 129

Query: 149 AATTRLKKWASHNTVELKDEIANMIFDLTAKRLISYDPKKSSENLRENFVAFIQGLISFP 208
            AT RLK+W+SHN VELKD+ A+MIFDLTAK+LISYD + SSENLR+NFVAFIQGLISFP
Sbjct: 130 VATRRLKQWSSHNPVELKDKTASMIFDLTAKKLISYDSENSSENLRDNFVAFIQGLISFP 189

Query: 209 LDIPGTSFHKCLQ-----------------QNPRKQHIDYFDYVLQELQKDGTPLTQEIA 268
           L++PGT+++KCLQ                  NPRKQ ID+FD+VL+EL+KDGT LT+EIA
Sbjct: 190 LNVPGTAYNKCLQGRKKAMRMLKNMLQERRTNPRKQRIDFFDFVLEELEKDGTLLTEEIA 249

Query: 269 LDLIFLLLFASYETTSIALTLAIKFLIDHPHVLNELTVHSFLDVSLVSSHPLLTYLISMQ 328
           LDL+F+LLFAS+ETTS+A+T AIKFL+++PHVL ELT                       
Sbjct: 250 LDLMFVLLFASFETTSLAITAAIKFLLNNPHVLEELT----------------------- 309

Query: 329 NLDHQAEHERILKERKNKSGGITWNEYKSMTFTFKVNEAFGKLFSCVIFLNETLRLANIA 388
                AEHE ILK R+     +TW EYKSMTFTF+             F+NET+RLANI 
Sbjct: 310 -----AEHEGILKRREISDSRLTWGEYKSMTFTFQ-------------FINETVRLANIV 369

Query: 389 PGIFRRALKDVEFKGYTIPEGWAVMRC---------------------------DGASKH 448
           PGIFR+ALKD+EFKGYTIP GWAVM C                           +G SKH
Sbjct: 370 PGIFRKALKDIEFKGYTIPSGWAVMVCPPAVHLNPEKYVDPLAFNPWRWEKSELNGGSKH 429

Query: 449 FMAFGGGMRFCIGADFAKMQMAVFLHNLVTNYTLKAIKGGNIVRTPGVQFPDGFHVEIVK 466
           FMAFGGGMRFC+G DF K+QMAVFLH LVT Y  KAIKGGNI+RTPG+QFPDGFHV+I++
Sbjct: 430 FMAFGGGMRFCVGTDFTKVQMAVFLHCLVTKYRFKAIKGGNIIRTPGLQFPDGFHVQIME 449

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C87A3_ORYSJ8.6e-13247.50Cytochrome P450 87A3 OS=Oryza sativa subsp. japonica GN=CYP87A3 PE=2 SV=3[more]
THAH_ARATH8.8e-6832.48Cytochrome P450 708A2 OS=Arabidopsis thaliana GN=CYP708A2 PE=2 SV=3[more]
C90A1_ARATH3.0e-5233.42Cytochrome P450 90A1 OS=Arabidopsis thaliana GN=CYP90A1 PE=2 SV=1[more]
C72B1_PINTA2.0e-5129.11Abietadienol/abietadienal oxidase OS=Pinus taeda GN=CYP720B1 PE=1 SV=1[more]
C90B1_ARATH2.6e-5128.54Cytochrome P450 90B1 OS=Arabidopsis thaliana GN=CYP90B1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0LCN3_CUCSA2.4e-19770.84Uncharacterized protein OS=Cucumis sativus GN=Csa_3G845470 PE=3 SV=1[more]
A0A0A0L5U2_CUCSA3.0e-16058.24Uncharacterized protein OS=Cucumis sativus GN=Csa_3G038790 PE=3 SV=1[more]
W9SLD3_9ROSA1.2e-15356.30Cytochrome P450 87A3 OS=Morus notabilis GN=L484_010567 PE=3 SV=1[more]
A0A067FJB9_CITSI1.6e-15355.88Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g040767mg PE=3 SV=1[more]
V4TQ27_9ROSI4.7e-15355.69Uncharacterized protein OS=Citrus clementina GN=CICLE_v10023269mg PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G12740.22.4e-14852.51 cytochrome P450, family 87, subfamily A, polypeptide 2[more]
AT1G78490.12.0e-7033.20 cytochrome P450, family 708, subfamily A, polypeptide 3[more]
AT5G48000.14.9e-6932.48 cytochrome P450, family 708, subfamily A, polypeptide 2[more]
AT1G55940.12.4e-6832.02 cytochrome P450, family 708, subfamily A, polypeptide 1[more]
AT3G44970.12.3e-6631.83 Cytochrome P450 superfamily protein[more]
Match NameE-valueIdentityDescription
gi|449446791|ref|XP_004141154.1|3.4e-19770.84PREDICTED: cytochrome P450 87A3-like [Cucumis sativus][more]
gi|659096178|ref|XP_008448963.1|2.1e-16258.82PREDICTED: cytochrome P450 87A3 isoform X1 [Cucumis melo][more]
gi|449460477|ref|XP_004147972.1|4.4e-16058.24PREDICTED: cytochrome P450 87A3 isoform X1 [Cucumis sativus][more]
gi|659096180|ref|XP_008448964.1|4.1e-15860.08PREDICTED: cytochrome P450 87A3 isoform X2 [Cucumis melo][more]
gi|778675642|ref|XP_011650442.1|2.2e-15659.46PREDICTED: cytochrome P450 87A3 isoform X2 [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0020037heme binding
GO:0005506iron ion binding
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Vocabulary: INTERPRO
TermDefinition
IPR017972Cyt_P450_CS
IPR002401Cyt_P450_E_grp-I
IPR001128Cyt_P450
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1901615 organic hydroxy compound metabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0065008 regulation of biological quality
biological_process GO:0008202 steroid metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G012770.1Lsi03G012770.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 261..278
score: 5.7E-8coord: 359..370
score: 5.7E-8coord: 415..426
score: 5.7E-8coord: 406..415
score: 5.
IPR001128Cytochrome P450GENE3DG3DSA:1.10.630.10coord: 14..465
score: 6.1
IPR001128Cytochrome P450PFAMPF00067p450coord: 401..447
score: 2.3E-10coord: 223..287
score: 4.3E-7coord: 361..396
score: 1.
IPR001128Cytochrome P450unknownSSF48264Cytochrome P450coord: 27..290
score: 9.14E-60coord: 354..452
score: 9.14
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 415..438
score: 1.3E-8coord: 405..415
score: 1.3E-8coord: 62..81
score: 1.
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 408..417
scor
NoneNo IPR availablePANTHERPTHR24286FAMILY NOT NAMEDcoord: 316..471
score: 2.6E-238coord: 1..287
score: 2.6E
NoneNo IPR availablePANTHERPTHR24286:SF11CYTOCHROME P450, FAMILY 87, SUBFAMILY A, POLYPEPTIDE 2coord: 316..471
score: 2.6E-238coord: 1..287
score: 2.6E