Lsi03G007930 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi03G007930
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionLaccase
Locationchr03 : 11619710 .. 11622438 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCAAGGAAAAACTTATCTTCTCCGAATTGTCAACGCGGTTATGGACGAAGATCTCTTCTTCGGAATCGCAAAACACGAAATGACATTAGTCGGAAAAGACGGGCTCTACACTAAACAAATCAAAACAAATTACCTAATGATCACACCAGGCCAATCCATGGACATATTGATCACTGCAAATCATTCCCCAGGAATCTATATTATGGCTACAAAATCCTACTCAAGTGCTTTTGGTGCTGGCTTTGATAACACCACAGCCACAGCCATTCTAAAGTACTCAACATCACCAAATCCCCAACTCAACCATTTCTTCCCACACTTACCTCCCTACGATAGAACCGAAGCAGCAACGAATTTCACCAAACGTCTACGAAGCTTGACTACTGTCGACGTTCCTTTAAACGTCGACACTCGTTTGTTTTTCGCGTTATCCGTCAATCTAATAGATTGCTCTAATGATCATAAGCCTTGTGCTGGGCCATTTGGGAAGAGATTTGCTGCAAGTATCAACAATGTGAGCTTTGTGACACCATCAGTTTCTTTGCTTGAAGCTTTCTACAACAACGTCGGTGGCGTGTTTACGACGGATTTCCTGGAAAACCCTCCGAGAAAATTCAACTACACCGGCGAGGTTCTACCCGAAAATTTGTTGGCTACTTCTTTTGGGACTAGGGTTATGGTTTTGGAGTATAATGCTAGTGTGGAGGTGATCTTGCAGGGTACTAATATTCTTGCAAGTGATAATCATCCCGTCCATTTGCATGGCTATAGCTTTTACGTTGTAGGATGGGGATTTGGGAATTTCGACCCTGAAACCGACCCGAAACGGTAAAATCTCATCGACCCACCGGAGGAGACGACGGTTGGAGTTCCTAAGAATGGCTGGGTTGCTATCAGATTCAAGGCAAATAATCCAGGTTTGTTTTTCAAGTACAATAATTCTGATGCAAACTTTAACTTTGATTTAATTTAAATTCTATTTACACTTGTTTTGAAATTTGGAAGTTAGTGTTTCCCTATGGTTTCTCCATCAATTTTCCGTTTTTTTTAGTGTACATGTTTTGAATAAAAAACTAAGGCTACTAACCATTTGGTTTTTAGTCTTTAGTTTTTGAAAATTAAATCTATTTTCTCTCCATCTTTTACAATGGTATATTTATATGCTTATGTAAAATAGTTGAATTCTTAGCCAAATTCTAAAAGAAAAACTACTACTTTAAAAACTACTTTTCTTTTTTTAGTTTTTAAAAATTGGTTTAGTTTTTGAAAAAATGGGTTAAATTATATAACAAAACAAAAAAAATTTAGAAGTGAGGCTTAATTTTCAAAGACTAAAAATCAAAAACCAAAAATCAAGGCCTTGTTTGGTATCCATTTCCTTTTTCCTTTTTGGTTTTTGAAAAGTAAGTCTATTTTCTCTCAATTTCTTATAGTGATTTGCATCTTTCTTAAACGGAAGAGTTGATTTTTTAGCCAAATTCTAAAAACAAAAACAAGTTTTTTAAAAACTACTTTTTTTTAGTTTTCAAATTTTGGTTTTGTTTTTTAAAACACTAGTAAAAAGTAGATAACAAATCAAAAAATTTATAGGTGGTAGGAGTGTTTATAAGCTTCATTTTCAGAAACAAAAAATAAAAAACCAAATGATTACCAAACAGAGCCCAAATGTTACCAAACGGGGAGTAAGTTTTTTTTTTTGAACATTTAATAAAAATATTAATCTAAACTTACGTTTAAGAAAATATGTTTAAAAGATTAAATGTATTATAATCTTGGTTAAATTATAAATTTGGTCCCATAATTAAGAGAAAGTTAGAATTTGATCCCTATAATTTTAAAACTTAAAATTTAGTCGTTATGATTTGATAAAACCTCATAAATAATAGTCTATATGGTTTGTTAAACCCCTCCGAAATAGTATCTACCATAGGGACTAAATTATACTTATAAACCATATGAACTAAATTCTAACTAATTTCTTTAAAACATAAGGACCAAATTTATAATTTAACAATCTATAAATCTATTGTTACATCGATCAGGAGGTCAAAAGTTTGAATCTTCTACTATGCATATTGCTGTACTAATAAAAACAAACTACTACATATCTAACTTATTAAAGGAAAGAATAATGTATTGAATTAGATAATTAAATTGAAAGAATAATGCGTATTGAATTAGATATATAATTAAATTTATATTGATCCATATATATGTCAATGATTATTCATTATTAACTAAGCTCTTATTTAAAAGAGCATTTGCAATGGATAGCACTTTTAAGGTTAATAATTAAGTGTATAGCAATATTTTTAAAAAATTATAATTATATCAAAATCTATCGGCGGTAGACTCCTGTCTATCACTGATAGACTCTGAGAGTTAAATCTAAATTTTGCTATATCTTCATAAAAAAATTACATTATATTATATCTACTAATATTTTGGATATGATTACTATGTTTGCAACTGTCTCTATTTAAAACAGACTTTCTAAGCGTTTTTTTAACATTATTAATCATTCTAAATATGTCCTATATATGTTTGAAAAAACTAATTAAATCTCTCTTATTTGATACTTTTATAGGTATGTGGTTGATGCATTGTCATATAGAGCGTCACCAAGTATGGGGCATGGGCATGGTGTTCTTAGTGAAAAATGGACTTGCTTCACACCAACAAATTCTTAACCCTCCACATGATTTGCCTTCATGCTATGATTAG

mRNA sequence

ATGGAGCAAGGAAAAACTTATCTTCTCCGAATTGTCAACGCGGTTATGGACGAAGATCTCTTCTTCGGAATCGCAAAACACGAAATGACATTAGTCGGAAAAGACGGGCTCTACACTAAACAAATCAAAACAAATTACCTAATGATCACACCAGGCCAATCCATGGACATATTGATCACTGCAAATCATTCCCCAGGAATCTATATTATGGCTACAAAATCCTACTCAAGTGCTTTTGGTGCTGGCTTTGATAACACCACAGCCACAGCCATTCTAAAGTACTCAACATCACCAAATCCCCAACTCAACCATTTCTTCCCACACTTACCTCCCTACGATAGAACCGAAGCAGCAACGAATTTCACCAAACGTCTACGAAGCTTGACTACTGTCGACGTTCCTTTAAACGTCGACACTCGTTTGTTTTTCGCGTTATCCGTCAATCTAATAGATTGCTCTAATGATCATAAGCCTTGTGCTGGGCCATTTGGGAAGAGATTTGCTGCAAGTATCAACAATGTGAGCTTTGTGACACCATCAGTTTCTTTGCTTGAAGCTTTCTACAACAACGTCGGTGGCGTGTTTACGACGGATTTCCTGGAAAACCCTCCGAGAAAATTCAACTACACCGGCGAGGTTCTACCCGAAAATTTGTTGGCTACTTCTTTTGGGACTAGGGTTATGGTTTTGGAGTATAATGCTAGTGTGGAGGTGATCTTGCAGGGTACTAATATTCTTGCAAGTGATAATCATCCCGTCCATTTGCATGGCTATAGCTTTTACGTTGTAGGATGGGGATTTGGGAATTTCGACCCTGAAACCGACCCGAAACGATTCAAGGCAAATAATCCAGGTATGTGGTTGATGCATTGTCATATAGAGCGTCACCAAGTATGGGGCATGGGCATGGTGTTCTTAGTGAAAAATGGACTTGCTTCACACCAACAAATTCTTAACCCTCCACATGATTTGCCTTCATGCTATGATTAG

Coding sequence (CDS)

ATGGAGCAAGGAAAAACTTATCTTCTCCGAATTGTCAACGCGGTTATGGACGAAGATCTCTTCTTCGGAATCGCAAAACACGAAATGACATTAGTCGGAAAAGACGGGCTCTACACTAAACAAATCAAAACAAATTACCTAATGATCACACCAGGCCAATCCATGGACATATTGATCACTGCAAATCATTCCCCAGGAATCTATATTATGGCTACAAAATCCTACTCAAGTGCTTTTGGTGCTGGCTTTGATAACACCACAGCCACAGCCATTCTAAAGTACTCAACATCACCAAATCCCCAACTCAACCATTTCTTCCCACACTTACCTCCCTACGATAGAACCGAAGCAGCAACGAATTTCACCAAACGTCTACGAAGCTTGACTACTGTCGACGTTCCTTTAAACGTCGACACTCGTTTGTTTTTCGCGTTATCCGTCAATCTAATAGATTGCTCTAATGATCATAAGCCTTGTGCTGGGCCATTTGGGAAGAGATTTGCTGCAAGTATCAACAATGTGAGCTTTGTGACACCATCAGTTTCTTTGCTTGAAGCTTTCTACAACAACGTCGGTGGCGTGTTTACGACGGATTTCCTGGAAAACCCTCCGAGAAAATTCAACTACACCGGCGAGGTTCTACCCGAAAATTTGTTGGCTACTTCTTTTGGGACTAGGGTTATGGTTTTGGAGTATAATGCTAGTGTGGAGGTGATCTTGCAGGGTACTAATATTCTTGCAAGTGATAATCATCCCGTCCATTTGCATGGCTATAGCTTTTACGTTGTAGGATGGGGATTTGGGAATTTCGACCCTGAAACCGACCCGAAACGATTCAAGGCAAATAATCCAGGTATGTGGTTGATGCATTGTCATATAGAGCGTCACCAAGTATGGGGCATGGGCATGGTGTTCTTAGTGAAAAATGGACTTGCTTCACACCAACAAATTCTTAACCCTCCACATGATTTGCCTTCATGCTATGATTAG

Protein sequence

MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILITANHSPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSPNPQLNHFFPHLPPYDRTEAATNFTKRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDHKPCAGPFGKRFAASINNVSFVTPSVSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNASVEVILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPKRFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSCYD
BLAST of Lsi03G007930 vs. Swiss-Prot
Match: LAC14_ARATH (Laccase-14 OS=Arabidopsis thaliana GN=LAC14 PE=2 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 1.3e-115
Identity = 198/348 (56.90%), Postives = 251/348 (72.13%), Query Frame = 1

Query: 3   QGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILITAN 62
           +G+ YLLRI+NAVMDE+LFF IA H +T+V KDG Y K  K++YLMITPGQSMD+L+ AN
Sbjct: 226 RGRRYLLRIINAVMDEELFFAIANHTLTVVAKDGFYLKHFKSDYLMITPGQSMDVLLHAN 285

Query: 63  HSPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSPNPQLNHFFPHLPPYDRTEAATNFT 122
             P  Y +A ++YSSAFGAGFD TT TAIL+Y      ++    P+LPPY+RTEA+T FT
Sbjct: 286 QRPNHYFVAARAYSSAFGAGFDKTTTTAILQYKGDTLNRIKPILPYLPPYNRTEASTRFT 345

Query: 123 KRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDHKPCAGPFGKRFAASINNVSFVTPSVS 182
            + RS   V+VP+ ++TRL +A+SVNL++CS+D +PC GPFGKRF++SINN+SFV PSV 
Sbjct: 346 NQFRSQRPVNVPVKINTRLLYAISVNLMNCSDD-RPCTGPFGKRFSSSINNISFVNPSVD 405

Query: 183 LLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNASVEVILQG 242
           +L A+Y ++GGVF  DF  NPP KFNYTGE LP     T FGT+V+VL+YN+SVE+ILQG
Sbjct: 406 ILRAYYRHIGGVFQEDFPRNPPTKFNYTGENLP---FPTRFGTKVVVLDYNSSVELILQG 465

Query: 243 TNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPKR-----------------------F 302
           T + AS+ HP+HLHGY+FYVVG GFGNFD   DP R                       F
Sbjct: 466 TTVWASNIHPIHLHGYNFYVVGSGFGNFDRRKDPLRYNLVDPPEETTVGVPRNGWTAVRF 525

Query: 303 KANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSC 328
            ANNPG+WL+HCHIERH  WGM  VF+VK+G     +++ PP DLPSC
Sbjct: 526 VANNPGVWLLHCHIERHATWGMNTVFIVKDGPTKSSRMVKPPPDLPSC 569

BLAST of Lsi03G007930 vs. Swiss-Prot
Match: LAC3_ARATH (Laccase-3 OS=Arabidopsis thaliana GN=LAC3 PE=2 SV=2)

HSP 1 Score: 323.2 bits (827), Expect = 3.4e-87
Identity = 167/355 (47.04%), Postives = 220/355 (61.97%), Query Frame = 1

Query: 4   GKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILITANH 63
           G+T  LR++NA M+++LFF +A H+ T+V  D  YTK   TN +MI PGQ+ ++L+TAN 
Sbjct: 218 GETVQLRVINAGMNQELFFSVANHQFTVVETDSAYTKPFTTNVIMIGPGQTTNVLLTANQ 277

Query: 64  SPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSP------NPQLNHFFPHLPPYDRTEA 123
            PG Y MA ++Y+SA  A FDNTT TAIL+Y  +P        Q+   FP LP ++ T  
Sbjct: 278 RPGRYYMAARAYNSA-NAPFDNTTTTAILQYVNAPTRRGRGRGQIAPVFPVLPGFNDTAT 337

Query: 124 ATNFTKRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDHKP-CAGPFGKRFAASINNVSF 183
           AT FT RLR      VP  VD  LFF + + LI+C+N + P C GP G RFAAS+NN+SF
Sbjct: 338 ATAFTNRLRYWKRAPVPQQVDENLFFTVGLGLINCANPNSPRCQGPNGTRFAASMNNMSF 397

Query: 184 VTP-SVSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNAS 243
           V P S S+++A+Y    G+FTTDF   PP +F+YTG V    L     GT+   L+Y ++
Sbjct: 398 VLPRSNSVMQAYYQGTPGIFTTDFPPVPPVQFDYTGNV-SRGLWQPIKGTKAYKLKYKSN 457

Query: 244 VEVILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPKRFK--------------- 303
           V+++LQ T+I+  +NHP+HLHGY FYVVG GFGNF+P TDP RF                
Sbjct: 458 VQIVLQDTSIVTPENHPMHLHGYQFYVVGSGFGNFNPRTDPARFNLFDPPERNTIGTPPG 517

Query: 304 --------ANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSC 328
                   A+NPG W MHCHI+ H  WG+ MVFLV+NG    Q +  PP DLP C
Sbjct: 518 GWVAIRFVADNPGAWFMHCHIDSHLGWGLAMVFLVENGRGQLQSVQAPPLDLPRC 570

BLAST of Lsi03G007930 vs. Swiss-Prot
Match: LAC12_ARATH (Laccase-12 OS=Arabidopsis thaliana GN=LAC12 PE=2 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 6.6e-83
Identity = 157/353 (44.48%), Postives = 214/353 (60.62%), Query Frame = 1

Query: 1   MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILIT 60
           +  G+T LLR++NA +++ LFF +A H++T+VG D  Y K   T  LM+ PGQ+ D+L+T
Sbjct: 214 INSGETSLLRVINAALNQPLFFTVANHKLTVVGADASYLKPFTTKVLMLGPGQTTDVLLT 273

Query: 61  ANHSPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSPNPQLNHFFPHLPPYDRTEAATN 120
           A+  P  Y +A ++Y SA  A FDNTT TAIL+Y  +         P LP ++ T   T+
Sbjct: 274 ADQPPKRYYIAARAYQSAQNAPFDNTTTTAILQYKKTTTTS-KPIMPVLPAFNDTNTVTS 333

Query: 121 FTKRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDHKP--CAGPFGKRFAASINNVSFVT 180
           F+++ +SL  V VP  +D  LFF + + L +C        C G  G RF AS+NNVSFV 
Sbjct: 334 FSRKFKSLRNVVVPKTIDDNLFFTIGLGLDNCPKKFPKSRCQGLNGTRFTASMNNVSFVL 393

Query: 181 PS-VSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNASVE 240
           PS  SLL+A  N + GVFTTDF   PP KF+YTG  +   L     GT++  L+Y + V+
Sbjct: 394 PSNFSLLQAHSNGIPGVFTTDFPSKPPVKFDYTGNNISRALFQPVKGTKLYKLKYGSRVQ 453

Query: 241 VILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPE--------TDPK------------ 300
           V+LQ TNI+ S+NHP+HLHGY FY+VG GFGNF+P+         DP             
Sbjct: 454 VVLQDTNIVTSENHPIHLHGYDFYIVGEGFGNFNPKKDTSKFNLVDPPLRNTVAVPVNGW 513

Query: 301 ---RFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSC 328
              RF A+NPG+WLMHCH++ H  WG+ M FLV NG+   + +  PPHDLP C
Sbjct: 514 AVIRFVADNPGVWLMHCHLDVHIKWGLAMAFLVDNGVGELETLEAPPHDLPIC 565

BLAST of Lsi03G007930 vs. Swiss-Prot
Match: LAC17_ORYSJ (Putative laccase-17 OS=Oryza sativa subsp. japonica GN=LAC17 PE=3 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 1.5e-82
Identity = 159/351 (45.30%), Postives = 211/351 (60.11%), Query Frame = 1

Query: 4   GKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILITANH 63
           G+T LLR +NA ++ +LF  +A H MT+V  D  YTK   T+ L++ PGQ+ D+L+T + 
Sbjct: 217 GETNLLRFINAALNTELFVSLAGHNMTVVAADASYTKPYTTSLLLLAPGQTTDVLVTFDQ 276

Query: 64  SPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSPNPQLNHF-FPHLPPYDRTEAATNFT 123
            PG Y +A ++Y+SA G  FDNTT TAI  Y  + N        P LP Y+ T AAT FT
Sbjct: 277 PPGRYYLAARAYASAQGVPFDNTTTTAIFDYGAANNASSAAIAMPTLPAYNDTTAATAFT 336

Query: 124 KRLRSLTTVDVPLNVDTRLFFALSVNLIDCSN-DHKPCAGPFGKRFAASINNVSFVTPS- 183
             LR L   ++P  VD  LFF + V L +C+N   + C GP G RFAASINNVSFV PS 
Sbjct: 337 TNLRGLRKAELPSRVDESLFFTVGVGLFNCTNATAQQCGGPNGTRFAASINNVSFVLPSS 396

Query: 184 VSLLEAFYNNV-GGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNASVEVI 243
            S+L+A ++   GGVFT DF  NPP +F+YT + +   L     GT+V  L+Y ++V+V+
Sbjct: 397 TSILQAHHHGAPGGVFTADFPANPPVQFDYTAQNVSRALWQPVAGTKVYKLKYGSAVQVV 456

Query: 244 LQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK---------------------- 303
           LQGTNI A +NHP+HLHGY FY++  G GNFD   D                        
Sbjct: 457 LQGTNIFAGENHPIHLHGYDFYILAEGLGNFDAGADTGKFNVEDPPMRNTVGVPVNGWAV 516

Query: 304 -RFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSC 328
            RF A+NPG+WLMHCH++ H  WG+ M FLV +G+   Q +  PP DLP C
Sbjct: 517 IRFVADNPGVWLMHCHLDVHITWGLAMAFLVDDGVGELQSLEAPPPDLPLC 567

BLAST of Lsi03G007930 vs. Swiss-Prot
Match: LAC23_ORYSJ (Laccase-23 OS=Oryza sativa subsp. japonica GN=LAC23 PE=3 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 7.3e-82
Identity = 158/351 (45.01%), Postives = 211/351 (60.11%), Query Frame = 1

Query: 4   GKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILITANH 63
           G+T LLR +NA ++ +LF  +A H MT+V  D  YTK   T+ L++ PGQ+ D+L+T + 
Sbjct: 217 GETNLLRFINAALNTELFVSLAGHNMTVVAADASYTKPYTTSLLLLAPGQTTDVLVTFDQ 276

Query: 64  SPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSPNPQLNHF-FPHLPPYDRTEAATNFT 123
            PG Y +A ++Y+SA G  FDNTT TAI  Y  + N        P LP Y+ T AAT FT
Sbjct: 277 PPGRYYLAARAYASAQGVPFDNTTTTAIFDYGAANNASSAAIAMPTLPAYNDTTAATAFT 336

Query: 124 KRLRSLTTVDVPLNVDTRLFFALSVNLIDCSN-DHKPCAGPFGKRFAASINNVSFVTPS- 183
             LR L   ++P  VD  LFF + V L +C+N   + C GP G RFAASINNVSFV PS 
Sbjct: 337 TNLRGLRKAELPSRVDESLFFTVGVGLFNCTNATAQQCGGPNGTRFAASINNVSFVLPSS 396

Query: 184 VSLLEAFYNNV-GGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNASVEVI 243
            S+L+A ++   GGVFT DF  +PP +F+YT + +   L     GT+V  L+Y ++V+V+
Sbjct: 397 TSILQAHHHGAPGGVFTADFPASPPVQFDYTAQNVSRALWQPVPGTKVYKLKYGSAVQVV 456

Query: 244 LQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK---------------------- 303
           LQGTNI A +NHP+HLHGY FY++  G GNFD   D                        
Sbjct: 457 LQGTNIFAGENHPIHLHGYDFYILAEGLGNFDAGADTAKFNMEDPPMRNTVGVPVNGWAV 516

Query: 304 -RFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSC 328
            RF A+NPG+WLMHCH++ H  WG+ M FLV +G+   Q +  PP DLP C
Sbjct: 517 IRFVADNPGVWLMHCHLDVHITWGLAMAFLVDDGVGELQSLEAPPPDLPLC 567

BLAST of Lsi03G007930 vs. TrEMBL
Match: A0A0A0K3Y1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G058630 PE=4 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 3.1e-140
Identity = 253/313 (80.83%), Postives = 273/313 (87.22%), Query Frame = 1

Query: 1   MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILIT 60
           MEQGKTYLLRIV+AVMDE+LFFGIAKH+MTLVGKDG+YTKQ KT+Y+MITPGQSMDILIT
Sbjct: 1   MEQGKTYLLRIVSAVMDEELFFGIAKHKMTLVGKDGIYTKQFKTDYIMITPGQSMDILIT 60

Query: 61  ANHSPGIYIMATKSYSSAFGAGFDNTTATAILKYST--SPNPQLNHFFPHLPPYDRTEAA 120
           AN SPG+YIMAT+SYSSAFGAGFDNTTATAILKYST  SPNP  N FFPHLPPYDRTEAA
Sbjct: 61  ANQSPGMYIMATRSYSSAFGAGFDNTTATAILKYSTITSPNPP-NPFFPHLPPYDRTEAA 120

Query: 121 TNFTKRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDHKPCAGPFGKRFAASINNVSFVT 180
           T+FTKRLRSLTTVDV L VDTRLFF LSVNL+DCSN+ KPCAGPFGKRFAASINNVSFVT
Sbjct: 121 TDFTKRLRSLTTVDVSLEVDTRLFFTLSVNLMDCSNEDKPCAGPFGKRFAASINNVSFVT 180

Query: 181 PSVSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNASVEV 240
           PSVSLLEA++N +GGVFTTDF  NPPRKFNYTGE LPE LL TSFGT+VMVLEYNASVE+
Sbjct: 181 PSVSLLEAYHNKIGGVFTTDFPGNPPRKFNYTGENLPEKLLPTSFGTKVMVLEYNASVEL 240

Query: 241 ILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK--------------------- 289
           ILQGTN+LASDNHPVHLHGYSFYVVGWGFGNFDP+TDPK                     
Sbjct: 241 ILQGTNVLASDNHPVHLHGYSFYVVGWGFGNFDPKTDPKRYNLVDPPEETTVGVPKNGWV 300

BLAST of Lsi03G007930 vs. TrEMBL
Match: M5Y906_PRUPE (Laccase OS=Prunus persica GN=PRUPE_ppa016865mg PE=3 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 1.7e-130
Identity = 226/357 (63.31%), Postives = 275/357 (77.03%), Query Frame = 1

Query: 1   MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILIT 60
           +E GKTYLLRI++AVMDE+LFFGIA H+M LVG+DG YTKQ+ T+Y+MI PGQSMD+L+ 
Sbjct: 214 VEHGKTYLLRIISAVMDEELFFGIANHKMILVGRDGSYTKQVVTSYIMIAPGQSMDVLLQ 273

Query: 61  ANHSPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSPN--PQLNHF-FPHLPPYDRTEA 120
           AN  P  Y MA ++YSSA GAGFD T  TAILKY  S +  P+  H+ FPHLPPYDRT+A
Sbjct: 274 ANQPPSFYFMAARAYSSAIGAGFDKTMTTAILKYQASYHEPPEFPHYKFPHLPPYDRTQA 333

Query: 121 ATNFTKRLRSLTTVD----VPLNVDTRLFFALSVNLIDCSNDHKPCAGPFGKRFAASINN 180
           +T+FTKR+RSL T D    VPL+V+T LFF +SVNL++CSN  KPC GPFGKRFAAS+NN
Sbjct: 334 STDFTKRIRSLATKDHPTNVPLHVNTHLFFTISVNLLNCSN--KPCTGPFGKRFAASVNN 393

Query: 181 VSFVTPSVSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYN 240
           +SFV PS+ +L A+Y  + GVF  DF + PP +FNYTGE LPENLL  S+GT+V+VLEYN
Sbjct: 394 ISFVAPSIDILRAYYYKIPGVFENDFPKKPPMEFNYTGEDLPENLLTPSYGTKVVVLEYN 453

Query: 241 ASVEVILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK---------------- 300
           ASVE++LQGTN+LASDNHPVHLHGY+FYVVGWGFGNF+P+ DP                 
Sbjct: 454 ASVELVLQGTNVLASDNHPVHLHGYNFYVVGWGFGNFNPKKDPLNYNLVDPPEENTVGVH 513

Query: 301 -------RFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSC 328
                  RF+A+NPG+WLMHCHIERHQ WGM +VFLVKNG +   +IL PPHDLP+C
Sbjct: 514 KNGWVAIRFRADNPGVWLMHCHIERHQTWGMTVVFLVKNGNSPQNKILPPPHDLPTC 568

BLAST of Lsi03G007930 vs. TrEMBL
Match: B9GF92_POPTR (Laccase OS=Populus trichocarpa GN=POPTR_0001s21380g PE=3 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 1.8e-124
Identity = 220/352 (62.50%), Postives = 266/352 (75.57%), Query Frame = 1

Query: 1   MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILIT 60
           +EQGKTYLLRI+NAV+DE+LFF IAKH++T+VGKDG Y K   ++YLMITPGQ+MD+L  
Sbjct: 214 VEQGKTYLLRIINAVLDENLFFSIAKHKLTIVGKDGCYLKPFTSDYLMITPGQTMDVLFE 273

Query: 61  ANHSPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSPN-PQLNHFFPHLPPYDRTEAAT 120
           AN  P  Y MA+++YSSAFGAGFDNTT TAI++Y    + P+  HF P LPPY+RT+A+T
Sbjct: 274 ANQPPSHYSMASRAYSSAFGAGFDNTTTTAIVEYHGIYHLPKSPHFSP-LPPYNRTQAST 333

Query: 121 NFTKRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDHKPCAGPFGKRFAASINNVSFVT- 180
           +FTK+ RS     VP  VDTRLFF +SVNL++CS D KPCAGPFGKRFAAS+NN+SFV  
Sbjct: 334 DFTKQFRSPVKAHVPQKVDTRLFFTISVNLLNCSTD-KPCAGPFGKRFAASMNNISFVNP 393

Query: 181 PSVSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNASVEV 240
           PS+ +L+A+Y  V GVF  +F   PP +FNYT E LP NLL  SFGT V VL+YNASVE+
Sbjct: 394 PSLDILQAYYYGVAGVFERNFPRKPPNEFNYTAENLPANLLTPSFGTEVRVLKYNASVEI 453

Query: 241 ILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK--------------------- 300
           ILQGTN+LA+DNHP+HLHGYSFYVVGWGFGNFDP  DP                      
Sbjct: 454 ILQGTNVLAADNHPIHLHGYSFYVVGWGFGNFDPSKDPSRYNLVDPPEETTVGVPHNGWA 513

Query: 301 --RFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSC 328
             RF+A+NPG+WL+HCHIERH  WGMGMVFLVKNG++S  +IL PP DLP C
Sbjct: 514 AIRFRADNPGVWLLHCHIERHVTWGMGMVFLVKNGVSSQARILKPPRDLPRC 563

BLAST of Lsi03G007930 vs. TrEMBL
Match: G7ILB5_MEDTR (Laccase OS=Medicago truncatula GN=MTR_2g008330 PE=3 SV=2)

HSP 1 Score: 439.1 bits (1128), Expect = 4.7e-120
Identity = 211/356 (59.27%), Postives = 260/356 (73.03%), Query Frame = 1

Query: 1   MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILIT 60
           ++ GKTYLLRI+N VMDE+LFF I  H++T+VGKDGLY K IKT+Y+MITPGQSMDIL+ 
Sbjct: 225 VDYGKTYLLRIINGVMDEELFFAIKNHKLTVVGKDGLYLKPIKTDYIMITPGQSMDILME 284

Query: 61  ANHSPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSPNP--QLNHFFPHLPPYDRTEAA 120
           AN   G Y MA +SYSS FGAGFDNTT TA+L Y+ S +   +     P LPPY++T+A+
Sbjct: 285 ANQPLGHYFMAARSYSSTFGAGFDNTTTTALLIYTDSHHHHHKKKPILPQLPPYNKTQAS 344

Query: 121 TNFTKRLRSLTT----VDVPLNVDTRLFFALSVNLIDCSNDHKPCAGPFGKRFAASINNV 180
           T+FTK+ RSL T    ++VP  VDT L F +SVNL++C+ D KPC GPFGKRFAAS+NN+
Sbjct: 345 TSFTKKFRSLATKTHPINVPTEVDTHLLFTISVNLLNCTQD-KPCTGPFGKRFAASVNNI 404

Query: 181 SFVTPSVSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNA 240
           S V P +  L A+Y  + GVF  DF   P R+FNYT + LPE  L+TSFGT+V+VL+Y+A
Sbjct: 405 SLVHPYIDFLSAYYYKIPGVFEMDFPRIPTREFNYTADKLPEYFLSTSFGTKVLVLDYDA 464

Query: 241 SVEVILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK----------------- 300
           SVE++LQGTN+LASDNHPVHLHGYSFYV+GWGFGNFDP+ DPK                 
Sbjct: 465 SVELVLQGTNVLASDNHPVHLHGYSFYVIGWGFGNFDPKNDPKNYNLIDPPEETTVGVPN 524

Query: 301 ------RFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSC 328
                 RF+A+NPGMWL+HCHIERH  WGM MVFLVK+G     Q+L PP DLP C
Sbjct: 525 NGWVAIRFRADNPGMWLLHCHIERHATWGMSMVFLVKDGPNPQTQMLPPPRDLPKC 579

BLAST of Lsi03G007930 vs. TrEMBL
Match: A0A087G5T7_ARAAL (Uncharacterized protein OS=Arabis alpina GN=AALP_AA8G086000 PE=3 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 3.7e-117
Identity = 208/358 (58.10%), Postives = 258/358 (72.07%), Query Frame = 1

Query: 1   MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILIT 60
           ME+G+ YLLRI+NAVMDE+LFF IA H +T+V KDGLY K  KT YLMITPGQSMD+L+ 
Sbjct: 1   MERGRRYLLRIINAVMDEELFFAIANHTLTVVAKDGLYLKHFKTRYLMITPGQSMDVLLH 60

Query: 61  ANHSPGIYIMATKSYSSAFGAGFDNTTATAILKYS----TSPNPQLNHFFPHLPPYDRTE 120
           AN  P  Y +A ++YSSAFGAGFDNTT TAIL+Y     T  + Q     P+LPPY+ TE
Sbjct: 61  ANQQPDRYFLAARAYSSAFGAGFDNTTTTAILQYKGVPLTEESNQTPPVLPYLPPYNHTE 120

Query: 121 AATNFTKRLRSLTT----VDVPLNVDTRLFFALSVNLIDCSNDHKPCAGPFGKRFAASIN 180
           A T FT + RSL +    V+VP+ +DTRL +A+SVNL++CS+D KPC+GPFGKRF++S+N
Sbjct: 121 ATTRFTSQFRSLISDNRPVNVPVKIDTRLLYAISVNLLNCSDD-KPCSGPFGKRFSSSVN 180

Query: 181 NVSFVTPSVSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEY 240
           N+SFV PS+ +L A+Y  +GGVF  DF  NPP +FNYTGE LP     T FGT+V+VL+Y
Sbjct: 181 NISFVNPSIDILIAYYLRIGGVFEADFPRNPPTEFNYTGENLP---FTTRFGTKVVVLDY 240

Query: 241 NASVEVILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPKR-------------- 300
           N+SVE++LQGTN+LASDNHP+HLHGYSF+VVG GFGNFD   DP R              
Sbjct: 241 NSSVELVLQGTNVLASDNHPIHLHGYSFFVVGSGFGNFDRSKDPLRYNLVDPPEETTVGV 300

Query: 301 ---------FKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSC 328
                    F ANNPG+WL+HCHIERH  WGM  VF+VK+G     ++L PP DLPSC
Sbjct: 301 PRNGWTAVRFVANNPGVWLLHCHIERHATWGMNTVFIVKDGPTKSSRMLKPPSDLPSC 354

BLAST of Lsi03G007930 vs. TAIR10
Match: AT5G09360.1 (AT5G09360.1 laccase 14)

HSP 1 Score: 417.5 bits (1072), Expect = 7.4e-117
Identity = 198/348 (56.90%), Postives = 251/348 (72.13%), Query Frame = 1

Query: 3   QGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILITAN 62
           +G+ YLLRI+NAVMDE+LFF IA H +T+V KDG Y K  K++YLMITPGQSMD+L+ AN
Sbjct: 226 RGRRYLLRIINAVMDEELFFAIANHTLTVVAKDGFYLKHFKSDYLMITPGQSMDVLLHAN 285

Query: 63  HSPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSPNPQLNHFFPHLPPYDRTEAATNFT 122
             P  Y +A ++YSSAFGAGFD TT TAIL+Y      ++    P+LPPY+RTEA+T FT
Sbjct: 286 QRPNHYFVAARAYSSAFGAGFDKTTTTAILQYKGDTLNRIKPILPYLPPYNRTEASTRFT 345

Query: 123 KRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDHKPCAGPFGKRFAASINNVSFVTPSVS 182
            + RS   V+VP+ ++TRL +A+SVNL++CS+D +PC GPFGKRF++SINN+SFV PSV 
Sbjct: 346 NQFRSQRPVNVPVKINTRLLYAISVNLMNCSDD-RPCTGPFGKRFSSSINNISFVNPSVD 405

Query: 183 LLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNASVEVILQG 242
           +L A+Y ++GGVF  DF  NPP KFNYTGE LP     T FGT+V+VL+YN+SVE+ILQG
Sbjct: 406 ILRAYYRHIGGVFQEDFPRNPPTKFNYTGENLP---FPTRFGTKVVVLDYNSSVELILQG 465

Query: 243 TNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPKR-----------------------F 302
           T + AS+ HP+HLHGY+FYVVG GFGNFD   DP R                       F
Sbjct: 466 TTVWASNIHPIHLHGYNFYVVGSGFGNFDRRKDPLRYNLVDPPEETTVGVPRNGWTAVRF 525

Query: 303 KANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSC 328
            ANNPG+WL+HCHIERH  WGM  VF+VK+G     +++ PP DLPSC
Sbjct: 526 VANNPGVWLLHCHIERHATWGMNTVFIVKDGPTKSSRMVKPPPDLPSC 569

BLAST of Lsi03G007930 vs. TAIR10
Match: AT2G30210.1 (AT2G30210.1 laccase 3)

HSP 1 Score: 323.2 bits (827), Expect = 1.9e-88
Identity = 167/355 (47.04%), Postives = 220/355 (61.97%), Query Frame = 1

Query: 4   GKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILITANH 63
           G+T  LR++NA M+++LFF +A H+ T+V  D  YTK   TN +MI PGQ+ ++L+TAN 
Sbjct: 218 GETVQLRVINAGMNQELFFSVANHQFTVVETDSAYTKPFTTNVIMIGPGQTTNVLLTANQ 277

Query: 64  SPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSP------NPQLNHFFPHLPPYDRTEA 123
            PG Y MA ++Y+SA  A FDNTT TAIL+Y  +P        Q+   FP LP ++ T  
Sbjct: 278 RPGRYYMAARAYNSA-NAPFDNTTTTAILQYVNAPTRRGRGRGQIAPVFPVLPGFNDTAT 337

Query: 124 ATNFTKRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDHKP-CAGPFGKRFAASINNVSF 183
           AT FT RLR      VP  VD  LFF + + LI+C+N + P C GP G RFAAS+NN+SF
Sbjct: 338 ATAFTNRLRYWKRAPVPQQVDENLFFTVGLGLINCANPNSPRCQGPNGTRFAASMNNMSF 397

Query: 184 VTP-SVSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNAS 243
           V P S S+++A+Y    G+FTTDF   PP +F+YTG V    L     GT+   L+Y ++
Sbjct: 398 VLPRSNSVMQAYYQGTPGIFTTDFPPVPPVQFDYTGNV-SRGLWQPIKGTKAYKLKYKSN 457

Query: 244 VEVILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPKRFK--------------- 303
           V+++LQ T+I+  +NHP+HLHGY FYVVG GFGNF+P TDP RF                
Sbjct: 458 VQIVLQDTSIVTPENHPMHLHGYQFYVVGSGFGNFNPRTDPARFNLFDPPERNTIGTPPG 517

Query: 304 --------ANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSC 328
                   A+NPG W MHCHI+ H  WG+ MVFLV+NG    Q +  PP DLP C
Sbjct: 518 GWVAIRFVADNPGAWFMHCHIDSHLGWGLAMVFLVENGRGQLQSVQAPPLDLPRC 570

BLAST of Lsi03G007930 vs. TAIR10
Match: AT5G05390.1 (AT5G05390.1 laccase 12)

HSP 1 Score: 308.9 bits (790), Expect = 3.7e-84
Identity = 157/353 (44.48%), Postives = 214/353 (60.62%), Query Frame = 1

Query: 1   MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILIT 60
           +  G+T LLR++NA +++ LFF +A H++T+VG D  Y K   T  LM+ PGQ+ D+L+T
Sbjct: 214 INSGETSLLRVINAALNQPLFFTVANHKLTVVGADASYLKPFTTKVLMLGPGQTTDVLLT 273

Query: 61  ANHSPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSPNPQLNHFFPHLPPYDRTEAATN 120
           A+  P  Y +A ++Y SA  A FDNTT TAIL+Y  +         P LP ++ T   T+
Sbjct: 274 ADQPPKRYYIAARAYQSAQNAPFDNTTTTAILQYKKTTTTS-KPIMPVLPAFNDTNTVTS 333

Query: 121 FTKRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDHKP--CAGPFGKRFAASINNVSFVT 180
           F+++ +SL  V VP  +D  LFF + + L +C        C G  G RF AS+NNVSFV 
Sbjct: 334 FSRKFKSLRNVVVPKTIDDNLFFTIGLGLDNCPKKFPKSRCQGLNGTRFTASMNNVSFVL 393

Query: 181 PS-VSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNASVE 240
           PS  SLL+A  N + GVFTTDF   PP KF+YTG  +   L     GT++  L+Y + V+
Sbjct: 394 PSNFSLLQAHSNGIPGVFTTDFPSKPPVKFDYTGNNISRALFQPVKGTKLYKLKYGSRVQ 453

Query: 241 VILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPE--------TDPK------------ 300
           V+LQ TNI+ S+NHP+HLHGY FY+VG GFGNF+P+         DP             
Sbjct: 454 VVLQDTNIVTSENHPIHLHGYDFYIVGEGFGNFNPKKDTSKFNLVDPPLRNTVAVPVNGW 513

Query: 301 ---RFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSC 328
              RF A+NPG+WLMHCH++ H  WG+ M FLV NG+   + +  PPHDLP C
Sbjct: 514 AVIRFVADNPGVWLMHCHLDVHIKWGLAMAFLVDNGVGELETLEAPPHDLPIC 565

BLAST of Lsi03G007930 vs. TAIR10
Match: AT5G07130.1 (AT5G07130.1 laccase 13)

HSP 1 Score: 305.4 bits (781), Expect = 4.1e-83
Identity = 159/356 (44.66%), Postives = 215/356 (60.39%), Query Frame = 1

Query: 4   GKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILITANH 63
           G+  LLR++N+ ++++LFFG+A H++T+V  D  YTK   TN +M+ PGQ+ D+L+TA+ 
Sbjct: 216 GEIVLLRVINSALNQELFFGVANHKLTVVAADASYTKPFSTNVIMLGPGQTTDVLLTADQ 275

Query: 64  SPGIYIMATKSYSSAFGAGFDNTTATAILKY------STSPNPQLNHFFPHLPPYDRTEA 123
            P  Y MA  +Y+SA  A FDNTT TAILKY      +     Q       LP ++ T  
Sbjct: 276 PPAHYYMAAHAYNSA-NAAFDNTTTTAILKYKDASCVTLQAKSQARAIPAQLPGFNDTAT 335

Query: 124 ATNFTKRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDH-KPCAGPFGKRFAASINNVSF 183
           A  FT +++S + V VPL +D  LFF + + L +C   + + C GP G RF ASINNVSF
Sbjct: 336 AAAFTAQMKSPSKVKVPLEIDENLFFTVGLGLFNCPTPNTQRCQGPNGTRFTASINNVSF 395

Query: 184 VTPSV-SLLEAFYNNV-GGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNA 243
           V P   S+++A+Y     GVFTTDF   PP  F+YTG V    L   + GT+   L++N+
Sbjct: 396 VFPKQNSIMQAYYQGTPTGVFTTDFPPTPPVTFDYTGNV-SRGLWQPTRGTKAYKLKFNS 455

Query: 244 SVEVILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK----------------- 303
            V++ILQ T+I+ ++NHP+HLHGY FYVVG G GNF+P TD                   
Sbjct: 456 QVQIILQDTSIVTTENHPMHLHGYEFYVVGTGVGNFNPNTDTSSFNLIDPPRRNTIGTPP 515

Query: 304 ------RFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSC 328
                 RF ANNPG WLMHCHI+ H  WG+ MVFLV+NG    Q + +PP DLP C
Sbjct: 516 GGWVAIRFVANNPGAWLMHCHIDSHIFWGLAMVFLVENGEGHLQSVQSPPLDLPQC 569

BLAST of Lsi03G007930 vs. TAIR10
Match: AT2G40370.1 (AT2G40370.1 laccase 5)

HSP 1 Score: 303.5 bits (776), Expect = 1.6e-82
Identity = 151/362 (41.71%), Postives = 214/362 (59.12%), Query Frame = 1

Query: 4   GKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILITANH 63
           G+T LLR++N+ +++ LFF +A H++T+VG D  Y K   TN +++ PGQ+ D+LIT + 
Sbjct: 219 GETILLRVINSALNQPLFFTVANHKLTVVGADASYLKPFTTNVIVLGPGQTTDVLITGDQ 278

Query: 64  SPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSP---------NPQLNHF---FPHLPP 123
            P  Y MA ++Y SA  A F NTT TAIL+Y ++P           + N F    P LP 
Sbjct: 279 PPNRYYMAARAYQSAQNAPFGNTTTTAILQYKSAPCCGVGGGSGTKKGNSFKPIMPILPA 338

Query: 124 YDRTEAATNFTKRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDHKP--CAGPFGKRFAA 183
           Y+ T   T F++  RSL   +VP  +D  LF  + + L +C  + +   C GP G RF A
Sbjct: 339 YNDTNTVTRFSQSFRSLRRAEVPTEIDENLFVTIGLGLNNCPKNFRSRRCQGPNGTRFTA 398

Query: 184 SINNVSFVTPS-VSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVM 243
           S+NNVSF  PS  SLL+A ++ + GVFTTDF   PP KF+YTG  +  +L     GT++ 
Sbjct: 399 SMNNVSFALPSNYSLLQAHHHGIPGVFTTDFPAKPPVKFDYTGNNISRSLYQPDRGTKLY 458

Query: 244 VLEYNASVEVILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPKRFK-------- 303
            L+Y + V+++LQ T I+  +NHP+HLHGY FY++  GFGNF+P+ D  +F         
Sbjct: 459 KLKYGSRVQIVLQDTGIVTPENHPIHLHGYDFYIIAEGFGNFNPKKDTAKFNLEDPPLRN 518

Query: 304 ---------------ANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLP 328
                          A+NPG+W+MHCH++ H  WG+ M FLV+NG    Q I  PPHDLP
Sbjct: 519 TVGVPVNGWAVIRFIADNPGVWIMHCHLDAHISWGLAMAFLVENGNGVLQTIEQPPHDLP 578

BLAST of Lsi03G007930 vs. NCBI nr
Match: gi|659111019|ref|XP_008455534.1| (PREDICTED: laccase-14 [Cucumis melo])

HSP 1 Score: 589.7 bits (1519), Expect = 3.1e-165
Identity = 289/354 (81.64%), Postives = 311/354 (87.85%), Query Frame = 1

Query: 1   MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILIT 60
           ME+GKTYLLRIV+AVMDEDLFFGIAKH+MTLVGKDG+Y KQIKT+Y+MITPGQSMDILIT
Sbjct: 210 MEKGKTYLLRIVSAVMDEDLFFGIAKHKMTLVGKDGIYMKQIKTDYIMITPGQSMDILIT 269

Query: 61  ANHSPGIYIMATKSYSSAFGAGFDNTTATAILKYST--SPNPQLNHFFPHLPPYDRTEAA 120
           AN SPG+YIMAT+SYSSAFGAGFDNTTATAILKYST  SPNP  N FFPHLPPYDRTEAA
Sbjct: 270 ANQSPGMYIMATRSYSSAFGAGFDNTTATAILKYSTITSPNPP-NPFFPHLPPYDRTEAA 329

Query: 121 TNFTKRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDHKPCAGPFGKRFAASINNVSFVT 180
           T+FTKRLRSL TVDV LNVDTRLFF LSVNL+DCSN+ KPCAGPFGKRFAASINNVSFVT
Sbjct: 330 TDFTKRLRSLRTVDVSLNVDTRLFFTLSVNLMDCSNEDKPCAGPFGKRFAASINNVSFVT 389

Query: 181 PSVSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNASVEV 240
           PSVSLLEA++N VGGVFTTDF ENPPRKFNYTGE LP+ LL TSFGT+VMVLEYNASVE+
Sbjct: 390 PSVSLLEAYHNRVGGVFTTDFPENPPRKFNYTGENLPKKLLPTSFGTKVMVLEYNASVEL 449

Query: 241 ILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK--------------------- 300
           ILQGTN+LASDNHPVHLHGYSFYVVGWGFGNFDP+ DPK                     
Sbjct: 450 ILQGTNVLASDNHPVHLHGYSFYVVGWGFGNFDPKNDPKRYNLVDPPEETTVGVPKNGWV 509

Query: 301 --RFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSCYD 330
             RFKA+NPGMWLMHCHIERHQ WGM MVFLVK+GLA HQQIL+PPHDLPSCY+
Sbjct: 510 AIRFKADNPGMWLMHCHIERHQAWGMNMVFLVKDGLAPHQQILHPPHDLPSCYN 562

BLAST of Lsi03G007930 vs. NCBI nr
Match: gi|449438540|ref|XP_004137046.1| (PREDICTED: laccase-14 [Cucumis sativus])

HSP 1 Score: 587.4 bits (1513), Expect = 1.5e-164
Identity = 289/352 (82.10%), Postives = 309/352 (87.78%), Query Frame = 1

Query: 1   MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILIT 60
           MEQGKTYLLRIV+AVMDE+LFFGIAKH+MTLVGKDG+YTKQ KT+Y+MITPGQSMDILIT
Sbjct: 221 MEQGKTYLLRIVSAVMDEELFFGIAKHKMTLVGKDGIYTKQFKTDYIMITPGQSMDILIT 280

Query: 61  ANHSPGIYIMATKSYSSAFGAGFDNTTATAILKYST--SPNPQLNHFFPHLPPYDRTEAA 120
           AN SPG+YIMAT+SYSSAFGAGFDNTTATAILKYST  SPNP  N FFPHLPPYDRTEAA
Sbjct: 281 ANQSPGMYIMATRSYSSAFGAGFDNTTATAILKYSTITSPNPP-NPFFPHLPPYDRTEAA 340

Query: 121 TNFTKRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDHKPCAGPFGKRFAASINNVSFVT 180
           T+FTKRLRSLTTVDV L VDTRLFF LSVNL+DCSN+ KPCAGPFGKRFAASINNVSFVT
Sbjct: 341 TDFTKRLRSLTTVDVSLEVDTRLFFTLSVNLMDCSNEDKPCAGPFGKRFAASINNVSFVT 400

Query: 181 PSVSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNASVEV 240
           PSVSLLEA++N +GGVFTTDF  NPPRKFNYTGE LPE LL TSFGT+VMVLEYNASVE+
Sbjct: 401 PSVSLLEAYHNKIGGVFTTDFPGNPPRKFNYTGENLPEKLLPTSFGTKVMVLEYNASVEL 460

Query: 241 ILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK--------------------- 300
           ILQGTN+LASDNHPVHLHGYSFYVVGWGFGNFDP+TDPK                     
Sbjct: 461 ILQGTNVLASDNHPVHLHGYSFYVVGWGFGNFDPKTDPKRYNLVDPPEETTVGVPKNGWV 520

Query: 301 --RFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSC 328
             RFKANNPGMWLMHCHIERHQVWGM MVFLVK+GLA HQQIL+ PHDLPSC
Sbjct: 521 AIRFKANNPGMWLMHCHIERHQVWGMTMVFLVKDGLAPHQQILHSPHDLPSC 571

BLAST of Lsi03G007930 vs. NCBI nr
Match: gi|700188456|gb|KGN43689.1| (hypothetical protein Csa_7G058630 [Cucumis sativus])

HSP 1 Score: 506.1 bits (1302), Expect = 4.5e-140
Identity = 253/313 (80.83%), Postives = 273/313 (87.22%), Query Frame = 1

Query: 1   MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILIT 60
           MEQGKTYLLRIV+AVMDE+LFFGIAKH+MTLVGKDG+YTKQ KT+Y+MITPGQSMDILIT
Sbjct: 1   MEQGKTYLLRIVSAVMDEELFFGIAKHKMTLVGKDGIYTKQFKTDYIMITPGQSMDILIT 60

Query: 61  ANHSPGIYIMATKSYSSAFGAGFDNTTATAILKYST--SPNPQLNHFFPHLPPYDRTEAA 120
           AN SPG+YIMAT+SYSSAFGAGFDNTTATAILKYST  SPNP  N FFPHLPPYDRTEAA
Sbjct: 61  ANQSPGMYIMATRSYSSAFGAGFDNTTATAILKYSTITSPNPP-NPFFPHLPPYDRTEAA 120

Query: 121 TNFTKRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDHKPCAGPFGKRFAASINNVSFVT 180
           T+FTKRLRSLTTVDV L VDTRLFF LSVNL+DCSN+ KPCAGPFGKRFAASINNVSFVT
Sbjct: 121 TDFTKRLRSLTTVDVSLEVDTRLFFTLSVNLMDCSNEDKPCAGPFGKRFAASINNVSFVT 180

Query: 181 PSVSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNASVEV 240
           PSVSLLEA++N +GGVFTTDF  NPPRKFNYTGE LPE LL TSFGT+VMVLEYNASVE+
Sbjct: 181 PSVSLLEAYHNKIGGVFTTDFPGNPPRKFNYTGENLPEKLLPTSFGTKVMVLEYNASVEL 240

Query: 241 ILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK--------------------- 289
           ILQGTN+LASDNHPVHLHGYSFYVVGWGFGNFDP+TDPK                     
Sbjct: 241 ILQGTNVLASDNHPVHLHGYSFYVVGWGFGNFDPKTDPKRYNLVDPPEETTVGVPKNGWV 300

BLAST of Lsi03G007930 vs. NCBI nr
Match: gi|645224163|ref|XP_008218979.1| (PREDICTED: laccase-14 [Prunus mume])

HSP 1 Score: 474.6 bits (1220), Expect = 1.4e-130
Identity = 226/357 (63.31%), Postives = 278/357 (77.87%), Query Frame = 1

Query: 1   MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILIT 60
           +E GKTYLLRI++AVMDE+LFFGIA H+M LVG+DG YTKQI+T+Y+MI PGQSMD+L+ 
Sbjct: 214 VEHGKTYLLRIISAVMDEELFFGIANHKMILVGRDGSYTKQIETSYIMIAPGQSMDVLLQ 273

Query: 61  ANHSPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSPN--PQLNHF-FPHLPPYDRTEA 120
           AN SP  Y MA ++YSSA GAGFD T  TAI+KY  S +  P+  H+ FPHLPPYDRT+A
Sbjct: 274 ANKSPSFYFMAARAYSSAIGAGFDKTMTTAIIKYQASYHEPPEFPHYKFPHLPPYDRTQA 333

Query: 121 ATNFTKRLRSLTTVD----VPLNVDTRLFFALSVNLIDCSNDHKPCAGPFGKRFAASINN 180
           +T+FTKR+RSL T D    VPL+V+T LFF +SVNL++CSN  KPC GPFGKRFAAS+NN
Sbjct: 334 STDFTKRIRSLATKDHPTNVPLHVNTHLFFTISVNLLNCSN--KPCTGPFGKRFAASVNN 393

Query: 181 VSFVTPSVSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYN 240
           +SFV PS+ +L A+Y+ + GVF  DF + PP++FNYTGE LPENLL  S+ T+V+VLEYN
Sbjct: 394 ISFVAPSIDILRAYYHKIPGVFEKDFPKKPPKEFNYTGEDLPENLLTPSYCTKVVVLEYN 453

Query: 241 ASVEVILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK---------------- 300
           ASVE++LQGTN+LASDNHPVHLHGY+FYVVGWGFGNF+P+ DP                 
Sbjct: 454 ASVELVLQGTNVLASDNHPVHLHGYNFYVVGWGFGNFNPKKDPLNYNLVDPPEENTVGVH 513

Query: 301 -------RFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSC 328
                  RF+A+NPG+WLMHCHIERHQ WGM +VFLVKNG +   +IL PPHDLP+C
Sbjct: 514 KNGWVAIRFRADNPGVWLMHCHIERHQTWGMTVVFLVKNGNSPQNKILPPPHDLPTC 568

BLAST of Lsi03G007930 vs. NCBI nr
Match: gi|596297235|ref|XP_007227301.1| (hypothetical protein PRUPE_ppa016865mg [Prunus persica])

HSP 1 Score: 473.8 bits (1218), Expect = 2.5e-130
Identity = 226/357 (63.31%), Postives = 275/357 (77.03%), Query Frame = 1

Query: 1   MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILIT 60
           +E GKTYLLRI++AVMDE+LFFGIA H+M LVG+DG YTKQ+ T+Y+MI PGQSMD+L+ 
Sbjct: 214 VEHGKTYLLRIISAVMDEELFFGIANHKMILVGRDGSYTKQVVTSYIMIAPGQSMDVLLQ 273

Query: 61  ANHSPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSPN--PQLNHF-FPHLPPYDRTEA 120
           AN  P  Y MA ++YSSA GAGFD T  TAILKY  S +  P+  H+ FPHLPPYDRT+A
Sbjct: 274 ANQPPSFYFMAARAYSSAIGAGFDKTMTTAILKYQASYHEPPEFPHYKFPHLPPYDRTQA 333

Query: 121 ATNFTKRLRSLTTVD----VPLNVDTRLFFALSVNLIDCSNDHKPCAGPFGKRFAASINN 180
           +T+FTKR+RSL T D    VPL+V+T LFF +SVNL++CSN  KPC GPFGKRFAAS+NN
Sbjct: 334 STDFTKRIRSLATKDHPTNVPLHVNTHLFFTISVNLLNCSN--KPCTGPFGKRFAASVNN 393

Query: 181 VSFVTPSVSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYN 240
           +SFV PS+ +L A+Y  + GVF  DF + PP +FNYTGE LPENLL  S+GT+V+VLEYN
Sbjct: 394 ISFVAPSIDILRAYYYKIPGVFENDFPKKPPMEFNYTGEDLPENLLTPSYGTKVVVLEYN 453

Query: 241 ASVEVILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK---------------- 300
           ASVE++LQGTN+LASDNHPVHLHGY+FYVVGWGFGNF+P+ DP                 
Sbjct: 454 ASVELVLQGTNVLASDNHPVHLHGYNFYVVGWGFGNFNPKKDPLNYNLVDPPEENTVGVH 513

Query: 301 -------RFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSC 328
                  RF+A+NPG+WLMHCHIERHQ WGM +VFLVKNG +   +IL PPHDLP+C
Sbjct: 514 KNGWVAIRFRADNPGVWLMHCHIERHQTWGMTVVFLVKNGNSPQNKILPPPHDLPTC 568

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
LAC14_ARATH1.3e-11556.90Laccase-14 OS=Arabidopsis thaliana GN=LAC14 PE=2 SV=1[more]
LAC3_ARATH3.4e-8747.04Laccase-3 OS=Arabidopsis thaliana GN=LAC3 PE=2 SV=2[more]
LAC12_ARATH6.6e-8344.48Laccase-12 OS=Arabidopsis thaliana GN=LAC12 PE=2 SV=1[more]
LAC17_ORYSJ1.5e-8245.30Putative laccase-17 OS=Oryza sativa subsp. japonica GN=LAC17 PE=3 SV=1[more]
LAC23_ORYSJ7.3e-8245.01Laccase-23 OS=Oryza sativa subsp. japonica GN=LAC23 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K3Y1_CUCSA3.1e-14080.83Uncharacterized protein OS=Cucumis sativus GN=Csa_7G058630 PE=4 SV=1[more]
M5Y906_PRUPE1.7e-13063.31Laccase OS=Prunus persica GN=PRUPE_ppa016865mg PE=3 SV=1[more]
B9GF92_POPTR1.8e-12462.50Laccase OS=Populus trichocarpa GN=POPTR_0001s21380g PE=3 SV=1[more]
G7ILB5_MEDTR4.7e-12059.27Laccase OS=Medicago truncatula GN=MTR_2g008330 PE=3 SV=2[more]
A0A087G5T7_ARAAL3.7e-11758.10Uncharacterized protein OS=Arabis alpina GN=AALP_AA8G086000 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G09360.17.4e-11756.90 laccase 14[more]
AT2G30210.11.9e-8847.04 laccase 3[more]
AT5G05390.13.7e-8444.48 laccase 12[more]
AT5G07130.14.1e-8344.66 laccase 13[more]
AT2G40370.11.6e-8241.71 laccase 5[more]
Match NameE-valueIdentityDescription
gi|659111019|ref|XP_008455534.1|3.1e-16581.64PREDICTED: laccase-14 [Cucumis melo][more]
gi|449438540|ref|XP_004137046.1|1.5e-16482.10PREDICTED: laccase-14 [Cucumis sativus][more]
gi|700188456|gb|KGN43689.1|4.5e-14080.83hypothetical protein Csa_7G058630 [Cucumis sativus][more]
gi|645224163|ref|XP_008218979.1|1.4e-13063.31PREDICTED: laccase-14 [Prunus mume][more]
gi|596297235|ref|XP_007227301.1|2.5e-13063.31hypothetical protein PRUPE_ppa016865mg [Prunus persica][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005507copper ion binding
GO:0016491oxidoreductase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: INTERPRO
TermDefinition
IPR011706Cu-oxidase_2
IPR008972Cupredoxin
IPR002355Cu_oxidase_Cu_BS
IPR001117Cu-oxidase
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009809 lignin biosynthetic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0046274 lignin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0048046 apoplast
molecular_function GO:0005507 copper ion binding
molecular_function GO:0016722 oxidoreductase activity, oxidizing metal ions
molecular_function GO:0052716 hydroquinone:oxygen oxidoreductase activity
molecular_function GO:0016491 oxidoreductase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G007930.1Lsi03G007930.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 1..96
score: 1.3
IPR002355Multicopper oxidase, copper-binding sitePROSITEPS00080MULTICOPPER_OXIDASE2coord: 290..301
scor
IPR008972CupredoxinGENE3DG3DSA:2.60.40.420coord: 129..328
score: 1.1E-31coord: 2..128
score: 2.5
IPR008972CupredoxinunknownSSF49503Cupredoxinscoord: 2..115
score: 2.97E-23coord: 134..327
score: 5.45
IPR011706Multicopper oxidase, type 2PFAMPF07731Cu-oxidase_2coord: 203..278
score: 3.1
NoneNo IPR availablePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 1..329
score: 1.5E
NoneNo IPR availablePANTHERPTHR11709:SF88LACCASE-14coord: 1..329
score: 1.5E