Lsi03G006760 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi03G006760
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	GDSL esterase/lipase
Location	chr03 : 8581586 .. 8582777 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTGACGCTATTTATCAATTGGGCAACTCTATTTTTGATACCAACAATCTCATTCGAGAAAATCTCAACACTACTTTTTCTCACCTTCCTTACGGCGAAACGTTCTTCAATAATCCCACTGGTCGATGCTCCAATGGTCTACTTATGATCGATTACTTTGGTACTCATTCTTTTTAATTAGCCTTCTATATTCTAATTTTCTTTCATTAATTCATTGTATTGCTCCACGATAATTCATGACTAATAACATATAACGGTCTTTTCAATTTGTAGCTTTAGACGCGGGGCTCCCTTTGGTCAACCCTTATCTCAACAAAGATGCTTTGACAAATCATGGAGTCAATTTCGCAATGGCTGGTTCTACTGCTTTGTCTTCTAAACTTCTTTCTCAGAAAACAATATCTTCTCCTGTTACTAATTCTTCTCTCAACCGTCAACTTGATTGGATGTTCTCTCATTTCAACTCCATTTGTTACAATCAAAGAGGTAATTAATTAACTCTTCCATTTAATATATGTACATAAAGATATATTTTTTTTTAGTTTTTTTCTTTCCATTTTACGTTTTGTTTAATGTTGGACAATTTTCACAAATATTTACATGTTGTGTGTAGATTGCAACGAGAAATTACATAATACTTTGTTCTTGGTGTGTGAGATTGGCGGCAATGACTATAACTATGCACTCTTCCAAAGCAAAACTATTCAAGAGGTAGAAGATATGGTACCCGATGTCCAATCCATAAAGGCTGCTGTTGAGGTAATTTGTTGTTAAGAAATTCTTTTTTCATGTTTTCTTTTTTGCTACTGATTATATGAATATGGGAGAGAAGTTTTTCTTATTTACTTTTTTCTTTCTTCTCTTTTGATGTTTTTAGAGAGTAATAAGTTATGGTACTACACGAGTTGTTGTCCCTGGAAATTTTCCAATTGGATGTTTCTCCATCTATCTCACTGGATTCCAAACCAATGATACAACTGCATGTGATGAACTCCATTGTTTAAAGGATTTGAACAATTTTGCTAGCTATCACAATGATCAAATCAAGGAAATGATTGAAGCATTAAAGAAAGAGAATCCACACACTGTTATCGTTTATGGTGACTACTATAATGCATTTCTATGGATTATTCGTCGTGCTTTTATACTAGGTATGTTTTTTACACCACACAAAATCATGATATTATAA

mRNA sequence

ATGTTTGACGCTATTTATCAATTGGGCAACTCTATTTTTGATACCAACAATCTCATTCGAGAAAATCTCAACACTACTTTTTCTCACCTTCCTTACGGCGAAACGTTCTTCAATAATCCCACTGGTCGATGCTCCAATGGTCTACTTATGATCGATTACTTTGCTTTAGACGCGGGGCTCCCTTTGGTCAACCCTTATCTCAACAAAGATGCTTTGACAAATCATGGAGTCAATTTCGCAATGGCTGGTTCTACTGCTTTGTCTTCTAAACTTCTTTCTCAGAAAACAATATCTTCTCCTGTTACTAATTCTTCTCTCAACCGTCAACTTGATTGGATGTTCTCTCATTTCAACTCCATTTGTTACAATCAAAGAGATTGCAACGAGAAATTACATAATACTTTGTTCTTGGTGTGTGAGATTGGCGGCAATGACTATAACTATGCACTCTTCCAAAGCAAAACTATTCAAGAGGTAGAAGATATGGTACCCGATGTCCAATCCATAAAGGCTGCTGTTGAGAGAGTAATAAGTTATGGTACTACACGAGTTGTTGTCCCTGGAAATTTTCCAATTGGATGTTTCTCCATCTATCTCACTGGATTCCAAACCAATGATACAACTGCATGTGATGAACTCCATTGTTTAAAGGATTTGAACAATTTTGCTAGCTATCACAATGATCAAATCAAGGAAATGATTGAAGCATTAAAGAAAGAGAATCCACACACTGTTATCGTTTATGGTGACTACTATAATGCATTTCTATGGATTATTCGTCGTGCTTTTATACTAGGTATGTTTTTTACACCACACAAAATCATGATATTATAA

Coding sequence (CDS)

ATGTTTGACGCTATTTATCAATTGGGCAACTCTATTTTTGATACCAACAATCTCATTCGAGAAAATCTCAACACTACTTTTTCTCACCTTCCTTACGGCGAAACGTTCTTCAATAATCCCACTGGTCGATGCTCCAATGGTCTACTTATGATCGATTACTTTGCTTTAGACGCGGGGCTCCCTTTGGTCAACCCTTATCTCAACAAAGATGCTTTGACAAATCATGGAGTCAATTTCGCAATGGCTGGTTCTACTGCTTTGTCTTCTAAACTTCTTTCTCAGAAAACAATATCTTCTCCTGTTACTAATTCTTCTCTCAACCGTCAACTTGATTGGATGTTCTCTCATTTCAACTCCATTTGTTACAATCAAAGAGATTGCAACGAGAAATTACATAATACTTTGTTCTTGGTGTGTGAGATTGGCGGCAATGACTATAACTATGCACTCTTCCAAAGCAAAACTATTCAAGAGGTAGAAGATATGGTACCCGATGTCCAATCCATAAAGGCTGCTGTTGAGAGAGTAATAAGTTATGGTACTACACGAGTTGTTGTCCCTGGAAATTTTCCAATTGGATGTTTCTCCATCTATCTCACTGGATTCCAAACCAATGATACAACTGCATGTGATGAACTCCATTGTTTAAAGGATTTGAACAATTTTGCTAGCTATCACAATGATCAAATCAAGGAAATGATTGAAGCATTAAAGAAAGAGAATCCACACACTGTTATCGTTTATGGTGACTACTATAATGCATTTCTATGGATTATTCGTCGTGCTTTTATACTAGGTATGTTTTTTACACCACACAAAATCATGATATTATAA

Protein sequence

MFDAIYQLGNSIFDTNNLIRENLNTTFSHLPYGETFFNNPTGRCSNGLLMIDYFALDAGLPLVNPYLNKDALTNHGVNFAMAGSTALSSKLLSQKTISSPVTNSSLNRQLDWMFSHFNSICYNQRDCNEKLHNTLFLVCEIGGNDYNYALFQSKTIQEVEDMVPDVQSIKAAVERVISYGTTRVVVPGNFPIGCFSIYLTGFQTNDTTACDELHCLKDLNNFASYHNDQIKEMIEALKKENPHTVIVYGDYYNAFLWIIRRAFILGMFFTPHKIMIL

Homology

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BLAST of Lsi03G006760 vs. Swiss-Prot
Match: AAE_RAUSE (Acetylajmalan esterase OS=Rauvolfia serpentina GN=AAE PE=1 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 2.6e-56
Identity = 116/269 (43.12%), Postives = 162/269 (60.22%), Query Frame = 1


Query: 2   FDAIYQLGNSIFDTNNLIR---ENLNTTFSHLPYGETFFNNPTGRCSNGLLMIDYFALDA 61
           FD+IYQLG+S  DT NLIR   +    T +H PYGETF   PTGRCS+G L+ID+ A   
Sbjct: 27  FDSIYQLGDSFSDTGNLIRLPPDGPTFTAAHFPYGETFPGTPTGRCSDGRLIIDFIATAL 86

Query: 62  GLPLVNPYLNKDALTNHGVNFAMAGSTALSSKLLSQKTISSPVTNSSLNRQLDWMFSHFN 121
            LPL+NPYL ++    HGVNFA+AG+TAL    L+ + +     +S L+ QL+W  ++  
Sbjct: 87  NLPLLNPYLQQNVSFRHGVNFAVAGATALDRSFLAARGVQVSDIHSHLSAQLNWFRTYLG 146

Query: 122 SICYNQRDCNEKLHNTLFLVCEIGGNDYNYALFQSKTIQEVEDMVPDV-QSIKAAVERVI 181
           SIC   ++C+ KL N LF++  IG ND NYA F ++TI+E+   VP + +++  A   +I
Sbjct: 147 SICSTPKECSNKLKNALFILGNIGNNDVNYA-FPNRTIEEIRAYVPFITEAVANATREII 206

Query: 182 SYGTTRVVVPGNFPIGCFSIYLTGFQTNDTTACDELHCLKDLNNFASYHNDQIKEMIEAL 241
             G +RV+VPG FPIGC +  L           D+L CL  LNN + Y N   +  + +L
Sbjct: 207 RLGGSRVIVPGIFPIGCVARNLNFLNFFPDGDKDDLGCLSSLNNLSIYFNSLFQRALASL 266

Query: 242 KKENPHTVIVYGDYYNAFLWIIRRAFILG 267
             E P  VI+Y DYYNA+ ++ R    LG
Sbjct: 267 SIEFPQAVIIYADYYNAWRFLFRNGPALG 294

[Close]

BLAST of Lsi03G006760 vs. Swiss-Prot
Match: GDL8_ARATH (GDSL esterase/lipase At1g28590 OS=Arabidopsis thaliana GN=At1g28590 PE=2 SV=2)

HSP 1 Score: 208.8 bits (530), Expect = 7.8e-53
Identity = 118/276 (42.75%), Postives = 168/276 (60.87%), Query Frame = 1


Query: 2   FDAIYQLGNSIFDTNNLIR---ENLNTTFSHLPYGETFFNNPTGRCSNGLLMIDYFALDA 61
           F +I   G+SI DT NL+     N     +  PYGETFF++PTGR S+G L+ID+ A   
Sbjct: 34  FKSIISFGDSIADTGNLLGLSDPNDLPASAFPPYGETFFHHPTGRYSDGRLIIDFIAEFL 93

Query: 62  GLPLVNPYLN-KDALTNHGVNFAMAGSTALSSKLLSQKTISSPVTNSSLNRQLDWMFSHF 121
           G PLV P+   ++A    GVNFA+AG+TAL    L ++ I S +TN SL+ QL       
Sbjct: 94  GFPLVPPFYGCQNANFKKGVNFAVAGATALEPSFLEERGIHSTITNVSLSVQLRSFTESL 153

Query: 122 NSICYNQRDCNEKLHNTLFLVCEIGGNDYNYALFQSKTIQEVEDMVPDV-QSIKAAVERV 181
            ++C +  DC + + N L L+ EIGGNDYN+ALFQ K ++EVE++VP V  +I +A+  +
Sbjct: 154 PNLCGSPSDCRDMIENALILMGEIGGNDYNFALFQRKPVKEVEELVPFVIATISSAITEL 213

Query: 182 ISYGTTRVVVPGNFPIGCFSIYLTGFQTNDTTACDEL-HCLKDLNNFASYHNDQIKEMIE 241
           +  G    +VPGNFPIG  + YLT ++T++    D L  CLK LN+F+ Y+N Q++E + 
Sbjct: 214 VCMGGRTFLVPGNFPIGYSASYLTLYKTSNKEEYDPLTGCLKWLNDFSEYYNKQLQEELN 273

Query: 242 ALKKENPHTVIVYGDYYNAFLWIIRRAFILGMFFTP 272
            L+K  PH  I+Y DYYNA L + +     G    P
Sbjct: 274 GLRKLYPHVNIIYADYYNALLRLFQEPAKFGFMNRP 309

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BLAST of Lsi03G006760 vs. Swiss-Prot
Match: GDL6_ARATH (GDSL esterase/lipase At1g28570 OS=Arabidopsis thaliana GN=At1g28570 PE=2 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 7.8e-53
Identity = 115/276 (41.67%), Postives = 165/276 (59.78%), Query Frame = 1


Query: 2   FDAIYQLGNSIFDTNNLIRENLNTTF---SHLPYGETFFNNPTGRCSNGLLMIDYFALDA 61
           F +I   G+SI DT NL+  +  T     + LPYGETFF++PTGR SNG L+ID+ A   
Sbjct: 32  FKSIISFGDSIADTGNLLALSDPTNLPKVAFLPYGETFFHHPTGRFSNGRLIIDFIAEFL 91

Query: 62  GLPLVNP-YLNKDALTNHGVNFAMAGSTALSSKLLSQKTISSPVTNSSLNRQLDWMFSHF 121
           G PLV P Y +++A    GVNFA+ G+TAL    L ++ I  P TN SL  QL       
Sbjct: 92  GFPLVPPFYGSQNANFEKGVNFAVGGATALERSFLEERGIHFPYTNVSLAVQLSSFKESL 151

Query: 122 NSICYNQRDCNEKLHNTLFLVCEIGGNDYNYALFQSKTIQEVEDMVP-DVQSIKAAVERV 181
            ++C +  DC + + N+L L+ EIGGNDYNYA F  K I+E++++VP  +++I +A+  +
Sbjct: 152 PNLCVSPSDCRDMIENSLILMGEIGGNDYNYAFFVGKNIEEIKELVPLVIETISSAITEL 211

Query: 182 ISYGTTRVVVPGNFPIGCFSIYLTGFQTNDTTACDEL-HCLKDLNNFASYHNDQIKEMIE 241
           I  G    +VPG FP+GC   YL+ +QT++    D L  CLK LN F+ YH++Q++  + 
Sbjct: 212 IGMGGKTFLVPGEFPLGCSVAYLSLYQTSNIEEYDPLTGCLKWLNKFSEYHDEQLQAELN 271

Query: 242 ALKKENPHTVIVYGDYYNAFLWIIRRAFILGMFFTP 272
            L+K  PH  I+Y DYYN  L + +     G    P
Sbjct: 272 RLQKLYPHVNIIYADYYNTLLRLAQEPAKFGFISRP 307

[Close]

BLAST of Lsi03G006760 vs. Swiss-Prot
Match: GDL7_ARATH (GDSL esterase/lipase At1g28580 OS=Arabidopsis thaliana GN=At1g28580 PE=2 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 2.5e-51
Identity = 115/276 (41.67%), Postives = 158/276 (57.25%), Query Frame = 1


Query: 2   FDAIYQLGNSIFDTNNLIRENLNTTFSHL---PYGETFFNNPTGRCSNGLLMIDYFALDA 61
           F +I   G+SI DT NL+  +      H+   PYGE FF++PTGR SNG L+ID+ A   
Sbjct: 35  FKSIISFGDSIADTGNLLGLSDPKDLPHMAFPPYGENFFHHPTGRFSNGRLIIDFIAEFL 94

Query: 62  GLPLVNP-YLNKDALTNHGVNFAMAGSTALSSKLLSQKTISSPVTNSSLNRQLDWMFSHF 121
           GLPLV P Y + +A    GVNFA+ G+TAL    L  + I  P TN SL  QL+      
Sbjct: 95  GLPLVPPFYGSHNANFEKGVNFAVGGATALERSFLEDRGIHFPYTNVSLGVQLNSFKESL 154

Query: 122 NSICYNQRDCNEKLHNTLFLVCEIGGNDYNYALFQSKTIQEVEDMVP-DVQSIKAAVERV 181
            SIC +  DC + + N L L+ EIGGNDYNYA F  K I+E+++++P  + +I +A+  +
Sbjct: 155 PSICGSPSDCRDMIENALILMGEIGGNDYNYAFFVDKGIEEIKELMPLVITTISSAITEL 214

Query: 182 ISYGTTRVVVPGNFPIGCFSIYLTGFQTNDTTACDEL-HCLKDLNNFASYHNDQIKEMIE 241
           I  G    +VPG FP+GC  +YLT  QT++    D L  CLK LN F   H +Q++  + 
Sbjct: 215 IGMGGRTFLVPGEFPVGCSVLYLTSHQTSNMEEYDPLTGCLKWLNKFGENHGEQLRAELN 274

Query: 242 ALKKENPHTVIVYGDYYNAFLWIIRRAFILGMFFTP 272
            L+K  PH  I+Y DYYNA   + +     G    P
Sbjct: 275 RLQKLYPHVNIIYADYYNALFHLYQEPAKFGFMNRP 310

[Close]

BLAST of Lsi03G006760 vs. Swiss-Prot
Match: GDL9_ARATH (GDSL esterase/lipase At1g28600 OS=Arabidopsis thaliana GN=At1g28600 PE=2 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 3.6e-50
Identity = 115/277 (41.52%), Postives = 162/277 (58.48%), Query Frame = 1


Query: 2   FDAIYQLGNSIFDTNNLI----RENLNTTFSHLPYGETFFNNPTGRCSNGLLMIDYFALD 61
           F +I   G+SI DT NL+    R  L  T +  PYGETFF++PTGR  +G +++D+ A  
Sbjct: 29  FKSIISFGDSIADTGNLVGLSDRNQLPVT-AFPPYGETFFHHPTGRSCDGRIIMDFIAEF 88

Query: 62  AGLPLVNPYL-NKDALTNHGVNFAMAGSTALSSKLLSQKTISSPVTNSSLNRQLDWMFSH 121
            GLP V PY  +K+   + GVNFA+AG+TAL S  L ++ I  P TN SL  QL      
Sbjct: 89  VGLPYVPPYFGSKNRNFDKGVNFAVAGATALKSSFLKKRGIQ-PHTNVSLGVQLKSFKKS 148

Query: 122 FNSICYNQRDCNEKLHNTLFLVCEIGGNDYNYALFQSKTIQEVEDMVPDV-QSIKAAVER 181
             ++C +  DC + + N L L+ EIGGNDYN+  F  K ++EVE++VP V  SI + +  
Sbjct: 149 LPNLCGSPSDCRDMIGNALILMGEIGGNDYNFPFFNRKPVKEVEELVPFVIASISSTITE 208

Query: 182 VISYGTTRVVVPGNFPIGCFSIYLTGFQTNDTTACD-ELHCLKDLNNFASYHNDQIKEMI 241
           +I  G    +VPG FPIGC  +YLT ++T++    D    CLK LN F  YH++++K  +
Sbjct: 209 LIGMGGKTFLVPGEFPIGCSVVYLTLYKTSNKDEYDPSTGCLKWLNKFGEYHSEKLKVEL 268

Query: 242 EALKKENPHTVIVYGDYYNAFLWIIRRAFILGMFFTP 272
             L+K  PH  I+Y DYYN+ L I +     G    P
Sbjct: 269 NRLRKLYPHVNIIYADYYNSLLRIFKEPAKFGFMERP 303

[Close]

BLAST of Lsi03G006760 vs. TrEMBL
Match: A0A061FGU1_THECC (SGNH hydrolase-type esterase superfamily protein, putative OS=Theobroma cacao GN=TCM_034863 PE=4 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 1.0e-91
Identity = 167/268 (62.31%), Postives = 206/268 (76.87%), Query Frame = 1


Query: 2   FDAIYQLGNSIFDTNNLIRENLNTTFSHLPYGETFFNNPTGRCSNGLLMIDYFALDAGLP 61
           FDAIYQLG+SI DT NLIRE+  + F+ LPYG+TFF N TGRCSNGLLMIDY AL AG+P
Sbjct: 34  FDAIYQLGDSISDTGNLIREDPLSPFARLPYGQTFFKNATGRCSNGLLMIDYIALSAGIP 93

Query: 62  LVNPYLNKDAL--TNHGVNFAMAGSTALSSKLLSQKTISSPVTNSSLNRQLDWMFSHFNS 121
            + PYLN DAL     GVNFA+AGSTAL  + L++  + +PVTNSSL+RQLDWMFS+FN 
Sbjct: 94  FLEPYLNSDALFTRGRGVNFAVAGSTALPVETLAENNVLAPVTNSSLSRQLDWMFSYFNG 153

Query: 122 ICYNQRDCNEKLHNTLFLVCEIGGNDYNYALFQSKTIQEVEDMVPDV-QSIKAAVERVIS 181
           IC ++ DC +KL   LF+V EIGGNDYNYALFQ KT  EV  M+P V Q+IK AV RV+ 
Sbjct: 154 ICRDEEDCVKKLKTALFMVGEIGGNDYNYALFQGKTFDEVRSMIPKVIQAIKDAVARVVG 213

Query: 182 YGTTRVVVPGNFPIGCFSIYLTGFQTNDTTACDELHCLKDLNNFASYHNDQIKEMIEALK 241
           YG TRV+VPGNFPIGC  IYL+GFQ++D+TA D  HCLK LNN A +HN  +K  I+ L+
Sbjct: 214 YGATRVIVPGNFPIGCLPIYLSGFQSSDSTAYDRFHCLKGLNNLAIHHNKLLKHAIKELR 273

Query: 242 KENPHTVIVYGDYYNAFLWIIRRAFILG 267
           K+ P+ +IVYGDYYNA+  ++R+  +LG
Sbjct: 274 KDLPNAIIVYGDYYNAYQKLLRKVELLG 301

[Close]

BLAST of Lsi03G006760 vs. TrEMBL
Match: A0A061F1T9_THECC (SGNH hydrolase-type esterase superfamily protein, putative OS=Theobroma cacao GN=TCM_025945 PE=4 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 2.3e-91
Identity = 166/268 (61.94%), Postives = 206/268 (76.87%), Query Frame = 1


Query: 2   FDAIYQLGNSIFDTNNLIRENLNTTFSHLPYGETFFNNPTGRCSNGLLMIDYFALDAGLP 61
           FDAIYQLG+SI DT NLIRE+  + F  LPYG+ FF + TGRCSNGLLMID+ AL  G+P
Sbjct: 34  FDAIYQLGDSISDTGNLIREDPLSPFGRLPYGQNFFKDATGRCSNGLLMIDFLALSTGIP 93

Query: 62  LVNPYLNKDALTN--HGVNFAMAGSTALSSKLLSQKTISSPVTNSSLNRQLDWMFSHFNS 121
            + PYLN DAL    HGVNFA+AGSTAL  ++L++  + +PVTN+SL RQLDWMFS+FN 
Sbjct: 94  FLEPYLNSDALLTRGHGVNFAVAGSTALPVEILAENNVLAPVTNTSLRRQLDWMFSYFNG 153

Query: 122 ICYNQRDCNEKLHNTLFLVCEIGGNDYNYALFQSKTIQEVEDMVPDV-QSIKAAVERVIS 181
           IC +Q DC +KL  +LF+V EIGGNDYNYALFQ KT +EV  M+P V Q+IK AV RV+ 
Sbjct: 154 ICRDQEDCFKKLKTSLFMVGEIGGNDYNYALFQGKTFEEVRSMMPKVVQAIKDAVTRVVG 213

Query: 182 YGTTRVVVPGNFPIGCFSIYLTGFQTNDTTACDELHCLKDLNNFASYHNDQIKEMIEALK 241
           YG TRV+VPGNFPIGC  IYLTGFQ+N + A D  HCLK LN  A +HN+ +K  I+ L+
Sbjct: 214 YGATRVIVPGNFPIGCLPIYLTGFQSNHSAAYDGFHCLKGLNKLAIHHNNLLKRAIKELR 273

Query: 242 KENPHTVIVYGDYYNAFLWIIRRAFILG 267
           K+ P+ +IVYGDYYNAFL ++R+A +LG
Sbjct: 274 KDLPNAIIVYGDYYNAFLQLLRKAELLG 301

[Close]

BLAST of Lsi03G006760 vs. TrEMBL
Match: V4V456_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10003375mg PE=4 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 3.3e-90
Identity = 160/261 (61.30%), Postives = 208/261 (79.69%), Query Frame = 1


Query: 3   DAIYQLGNSIFDTNNLIRENLNTTFSHLPYGETFFNNPTGRCSNGLLMIDYFALDAGLPL 62
           ++IYQLG+S+ DT NLI+E+  + F+ LPYGETFF N TGRCSNGLLMIDYFA+ AG+P 
Sbjct: 37  NSIYQLGDSVSDTGNLIQEHPESAFAKLPYGETFFKNATGRCSNGLLMIDYFAISAGIPF 96

Query: 63  VNPYLNKDA---LTNHGVNFAMAGSTALSSKLLSQKTISSPVTNSSLNRQLDWMFSHFNS 122
           V+ +LN +A   +  HGVNFA+AGSTAL S +L+ K ISSPVTNSSL+ QLDWMF++FN 
Sbjct: 97  VDAFLNSNASLYIQGHGVNFAVAGSTALPSYILADKKISSPVTNSSLSTQLDWMFTYFNG 156

Query: 123 ICYNQRDCNEKLHNTLFLVCEIGGNDYNYALFQSKTIQEVEDMVPD-VQSIKAAVERVIS 182
           ICY+  DC +KL ++LF+V EIG NDY YALFQ KT++EV+ +VP+ VQ+IK AV RVI 
Sbjct: 157 ICYDYDDCAQKLKSSLFIVGEIGENDYKYALFQGKTVEEVKTLVPEVVQAIKDAVTRVID 216

Query: 183 YGTTRVVVPGNFPIGCFSIYLTGFQTNDTTACDELHCLKDLNNFASYHNDQIKEMIEALK 242
           +G  RVVVPGNFP+GCF IYLT F+TND+ A D+ HCLK LNN + +HN+ +++ I+ LK
Sbjct: 217 FGAVRVVVPGNFPVGCFPIYLTQFRTNDSAAYDQFHCLKCLNNLSFHHNELLQQAIQELK 276

Query: 243 KENPHTVIVYGDYYNAFLWII 260
            E+P+  I+YGDYY AF+WI+
Sbjct: 277 NEHPNVAIIYGDYYTAFMWIL 297

[Close]

BLAST of Lsi03G006760 vs. TrEMBL
Match: A0A061EYZ6_THECC (SGNH hydrolase-type esterase superfamily protein, putative OS=Theobroma cacao GN=TCM_025392 PE=4 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 9.5e-90
Identity = 165/267 (61.80%), Postives = 209/267 (78.28%), Query Frame = 1


Query: 2   FDAIYQLGNSIFDTNNLIRENLNTTFSHLPYGETFFNNPTGRCSNGLLMIDYFALDAGLP 61
           FDAI+Q+G+SI DT N I+EN ++ ++ LPYGETFF   TGRCSNGLLMIDY AL A +P
Sbjct: 40  FDAIHQVGDSISDTGNYIQENPSSVYARLPYGETFFKKATGRCSNGLLMIDYLALSAEIP 99

Query: 62  LVNPYLNKDALTNHGVNFAMAGSTALSSKLLSQKTISSPVTNSSLNRQLDWMFSHFNSIC 121
            ++ YLNKD L +HGVNFA+AG+TALS ++LS++ ISS  TN SL+ QLDWMFS+FN  C
Sbjct: 100 FLDAYLNKDGLFDHGVNFAVAGATALSVEVLSKRNISSVPTNKSLSNQLDWMFSYFNRTC 159

Query: 122 YNQRDCNEKLHNTLFLVCEIGGNDYNYAL-FQSKTIQEVEDMVPD-VQSIKAAVERVISY 181
              +DC  K    LF+V EIGGNDYNYA    + T++EVE MVP+ VQ+IK AV+RVI Y
Sbjct: 160 RYDKDCFIKNGRALFMVGEIGGNDYNYAFQIGNLTLEEVEAMVPEVVQAIKYAVKRVIGY 219

Query: 182 GTTRVVVPGNFPIGCFSIYLTGFQTNDTTACDELHCLKDLNNFASYHNDQIKEMIEALKK 241
           G TR+VVPGNFPIGCF +YLT FQTNDTTA D+LHCLK LNN + YHND +++ IE LK+
Sbjct: 220 GATRLVVPGNFPIGCFPVYLTKFQTNDTTAYDDLHCLKYLNNISIYHNDLLQQAIEELKE 279

Query: 242 ENPHTVIVYGDYYNAFLWIIRRAFILG 267
           E+P+ +IVYG+YY AF W++ +A +LG
Sbjct: 280 EHPNVIIVYGNYYTAFQWLLSKANLLG 306

[Close]

BLAST of Lsi03G006760 vs. TrEMBL
Match: U5G9P4_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0005s02420g PE=4 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 6.2e-89
Identity = 166/268 (61.94%), Postives = 206/268 (76.87%), Query Frame = 1


Query: 2   FDAIYQLGNSIFDTNNLIRENLNTTFSHLPYGETFFNNPTGRCSNGLLMIDYFALDAGLP 61
           FDAIYQLG+SI DT N I+E  ++ ++  PYGETFFN PTGRCSNG LMID+ A  AG+P
Sbjct: 36  FDAIYQLGDSISDTGNFIQEKPSSVYARFPYGETFFNKPTGRCSNGRLMIDFIASSAGVP 95

Query: 62  LVNPYLNKDAL--TNHGVNFAMAGSTALSSKLLSQKTISSPVTNSSLNRQLDWMFSHFNS 121
            ++ +LN +      HGVNFA+A STAL + +LS+K I  P T+SSL+ QLDWMFS+FNS
Sbjct: 96  FLDAHLNPNGTFTRGHGVNFAVASSTALPADILSKKNIFPP-THSSLSVQLDWMFSYFNS 155

Query: 122 ICYNQRDCNEKLHNTLFLVCEIGGNDYNYALFQSKTIQEVEDMVPDV-QSIKAAVERVIS 181
           IC+N++DC EKL N+LF+V EIG NDY YA FQ K ++EV++MVPDV Q+IK AV RVI 
Sbjct: 156 ICFNEQDCAEKLKNSLFMVGEIGVNDYTYAFFQGKIMEEVKNMVPDVVQAIKDAVTRVIG 215

Query: 182 YGTTRVVVPGNFPIGCFSIYLTGFQTNDTTACDELHCLKDLNNFASYHNDQIKEMIEALK 241
           YG  RVVVPGN PIGCF IYLTGFQTN+T A D+ HCLK LNN ++ HND +++ IE LK
Sbjct: 216 YGARRVVVPGNVPIGCFPIYLTGFQTNNTDAYDKFHCLKGLNNLSASHNDHLQQAIEELK 275

Query: 242 KENPHTVIVYGDYYNAFLWIIRRAFILG 267
           KENP+ +I Y DYYNAF WI+ +A  LG
Sbjct: 276 KENPNVLIAYADYYNAFQWILTKAPNLG 302

[Close]

BLAST of Lsi03G006760 vs. TAIR10
Match: AT1G28570.1 (AT1G28570.1 SGNH hydrolase-type esterase superfamily protein)

HSP 1 Score: 208.8 bits (530), Expect = 4.4e-54
Identity = 115/276 (41.67%), Postives = 165/276 (59.78%), Query Frame = 1


Query: 2   FDAIYQLGNSIFDTNNLIRENLNTTF---SHLPYGETFFNNPTGRCSNGLLMIDYFALDA 61
           F +I   G+SI DT NL+  +  T     + LPYGETFF++PTGR SNG L+ID+ A   
Sbjct: 32  FKSIISFGDSIADTGNLLALSDPTNLPKVAFLPYGETFFHHPTGRFSNGRLIIDFIAEFL 91

Query: 62  GLPLVNP-YLNKDALTNHGVNFAMAGSTALSSKLLSQKTISSPVTNSSLNRQLDWMFSHF 121
           G PLV P Y +++A    GVNFA+ G+TAL    L ++ I  P TN SL  QL       
Sbjct: 92  GFPLVPPFYGSQNANFEKGVNFAVGGATALERSFLEERGIHFPYTNVSLAVQLSSFKESL 151

Query: 122 NSICYNQRDCNEKLHNTLFLVCEIGGNDYNYALFQSKTIQEVEDMVP-DVQSIKAAVERV 181
            ++C +  DC + + N+L L+ EIGGNDYNYA F  K I+E++++VP  +++I +A+  +
Sbjct: 152 PNLCVSPSDCRDMIENSLILMGEIGGNDYNYAFFVGKNIEEIKELVPLVIETISSAITEL 211

Query: 182 ISYGTTRVVVPGNFPIGCFSIYLTGFQTNDTTACDEL-HCLKDLNNFASYHNDQIKEMIE 241
           I  G    +VPG FP+GC   YL+ +QT++    D L  CLK LN F+ YH++Q++  + 
Sbjct: 212 IGMGGKTFLVPGEFPLGCSVAYLSLYQTSNIEEYDPLTGCLKWLNKFSEYHDEQLQAELN 271

Query: 242 ALKKENPHTVIVYGDYYNAFLWIIRRAFILGMFFTP 272
            L+K  PH  I+Y DYYN  L + +     G    P
Sbjct: 272 RLQKLYPHVNIIYADYYNTLLRLAQEPAKFGFISRP 307

[Close]

BLAST of Lsi03G006760 vs. TAIR10
Match: AT1G28590.1 (AT1G28590.1 GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 208.8 bits (530), Expect = 4.4e-54
Identity = 118/276 (42.75%), Postives = 168/276 (60.87%), Query Frame = 1


Query: 2   FDAIYQLGNSIFDTNNLIR---ENLNTTFSHLPYGETFFNNPTGRCSNGLLMIDYFALDA 61
           F +I   G+SI DT NL+     N     +  PYGETFF++PTGR S+G L+ID+ A   
Sbjct: 34  FKSIISFGDSIADTGNLLGLSDPNDLPASAFPPYGETFFHHPTGRYSDGRLIIDFIAEFL 93

Query: 62  GLPLVNPYLN-KDALTNHGVNFAMAGSTALSSKLLSQKTISSPVTNSSLNRQLDWMFSHF 121
           G PLV P+   ++A    GVNFA+AG+TAL    L ++ I S +TN SL+ QL       
Sbjct: 94  GFPLVPPFYGCQNANFKKGVNFAVAGATALEPSFLEERGIHSTITNVSLSVQLRSFTESL 153

Query: 122 NSICYNQRDCNEKLHNTLFLVCEIGGNDYNYALFQSKTIQEVEDMVPDV-QSIKAAVERV 181
            ++C +  DC + + N L L+ EIGGNDYN+ALFQ K ++EVE++VP V  +I +A+  +
Sbjct: 154 PNLCGSPSDCRDMIENALILMGEIGGNDYNFALFQRKPVKEVEELVPFVIATISSAITEL 213

Query: 182 ISYGTTRVVVPGNFPIGCFSIYLTGFQTNDTTACDEL-HCLKDLNNFASYHNDQIKEMIE 241
           +  G    +VPGNFPIG  + YLT ++T++    D L  CLK LN+F+ Y+N Q++E + 
Sbjct: 214 VCMGGRTFLVPGNFPIGYSASYLTLYKTSNKEEYDPLTGCLKWLNDFSEYYNKQLQEELN 273

Query: 242 ALKKENPHTVIVYGDYYNAFLWIIRRAFILGMFFTP 272
            L+K  PH  I+Y DYYNA L + +     G    P
Sbjct: 274 GLRKLYPHVNIIYADYYNALLRLFQEPAKFGFMNRP 309

[Close]

BLAST of Lsi03G006760 vs. TAIR10
Match: AT1G28580.1 (AT1G28580.1 GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 203.8 bits (517), Expect = 1.4e-52
Identity = 115/276 (41.67%), Postives = 158/276 (57.25%), Query Frame = 1


Query: 2   FDAIYQLGNSIFDTNNLIRENLNTTFSHL---PYGETFFNNPTGRCSNGLLMIDYFALDA 61
           F +I   G+SI DT NL+  +      H+   PYGE FF++PTGR SNG L+ID+ A   
Sbjct: 35  FKSIISFGDSIADTGNLLGLSDPKDLPHMAFPPYGENFFHHPTGRFSNGRLIIDFIAEFL 94

Query: 62  GLPLVNP-YLNKDALTNHGVNFAMAGSTALSSKLLSQKTISSPVTNSSLNRQLDWMFSHF 121
           GLPLV P Y + +A    GVNFA+ G+TAL    L  + I  P TN SL  QL+      
Sbjct: 95  GLPLVPPFYGSHNANFEKGVNFAVGGATALERSFLEDRGIHFPYTNVSLGVQLNSFKESL 154

Query: 122 NSICYNQRDCNEKLHNTLFLVCEIGGNDYNYALFQSKTIQEVEDMVP-DVQSIKAAVERV 181
            SIC +  DC + + N L L+ EIGGNDYNYA F  K I+E+++++P  + +I +A+  +
Sbjct: 155 PSICGSPSDCRDMIENALILMGEIGGNDYNYAFFVDKGIEEIKELMPLVITTISSAITEL 214

Query: 182 ISYGTTRVVVPGNFPIGCFSIYLTGFQTNDTTACDEL-HCLKDLNNFASYHNDQIKEMIE 241
           I  G    +VPG FP+GC  +YLT  QT++    D L  CLK LN F   H +Q++  + 
Sbjct: 215 IGMGGRTFLVPGEFPVGCSVLYLTSHQTSNMEEYDPLTGCLKWLNKFGENHGEQLRAELN 274

Query: 242 ALKKENPHTVIVYGDYYNAFLWIIRRAFILGMFFTP 272
            L+K  PH  I+Y DYYNA   + +     G    P
Sbjct: 275 RLQKLYPHVNIIYADYYNALFHLYQEPAKFGFMNRP 310

[Close]

BLAST of Lsi03G006760 vs. TAIR10
Match: AT1G28600.1 (AT1G28600.1 GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 199.9 bits (507), Expect = 2.0e-51
Identity = 115/277 (41.52%), Postives = 162/277 (58.48%), Query Frame = 1


Query: 2   FDAIYQLGNSIFDTNNLI----RENLNTTFSHLPYGETFFNNPTGRCSNGLLMIDYFALD 61
           F +I   G+SI DT NL+    R  L  T +  PYGETFF++PTGR  +G +++D+ A  
Sbjct: 29  FKSIISFGDSIADTGNLVGLSDRNQLPVT-AFPPYGETFFHHPTGRSCDGRIIMDFIAEF 88

Query: 62  AGLPLVNPYL-NKDALTNHGVNFAMAGSTALSSKLLSQKTISSPVTNSSLNRQLDWMFSH 121
            GLP V PY  +K+   + GVNFA+AG+TAL S  L ++ I  P TN SL  QL      
Sbjct: 89  VGLPYVPPYFGSKNRNFDKGVNFAVAGATALKSSFLKKRGIQ-PHTNVSLGVQLKSFKKS 148

Query: 122 FNSICYNQRDCNEKLHNTLFLVCEIGGNDYNYALFQSKTIQEVEDMVPDV-QSIKAAVER 181
             ++C +  DC + + N L L+ EIGGNDYN+  F  K ++EVE++VP V  SI + +  
Sbjct: 149 LPNLCGSPSDCRDMIGNALILMGEIGGNDYNFPFFNRKPVKEVEELVPFVIASISSTITE 208

Query: 182 VISYGTTRVVVPGNFPIGCFSIYLTGFQTNDTTACD-ELHCLKDLNNFASYHNDQIKEMI 241
           +I  G    +VPG FPIGC  +YLT ++T++    D    CLK LN F  YH++++K  +
Sbjct: 209 LIGMGGKTFLVPGEFPIGCSVVYLTLYKTSNKDEYDPSTGCLKWLNKFGEYHSEKLKVEL 268

Query: 242 EALKKENPHTVIVYGDYYNAFLWIIRRAFILGMFFTP 272
             L+K  PH  I+Y DYYN+ L I +     G    P
Sbjct: 269 NRLRKLYPHVNIIYADYYNSLLRIFKEPAKFGFMERP 303

[Close]

BLAST of Lsi03G006760 vs. TAIR10
Match: AT1G28650.1 (AT1G28650.1 GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 197.2 bits (500), Expect = 1.3e-50
Identity = 113/278 (40.65%), Postives = 165/278 (59.35%), Query Frame = 1


Query: 2   FDAIYQLGNSIFDTNNLIR-ENLNTT--FSHLPYGETFFNNPTGRCSNGLLMIDYFALDA 61
           + +I   G+SI DT N +   N+N     + LPYGE+FF+ P+GR S+G L+ID+ A   
Sbjct: 35  YKSIISFGDSIADTGNYVHLSNVNNLPQAAFLPYGESFFHPPSGRYSDGRLVIDFIAEFL 94

Query: 62  GLPLVNPYLNKDALT-NHGVNFAMAGSTALSSKLLSQKTISSPVTNSSLNRQLDWMFSHF 121
           GLP V PY     ++ N G+NFA+ G+TAL    L ++ I S  TN SL+ QL+      
Sbjct: 95  GLPYVPPYFGSQNVSFNQGINFAVYGATALDRAFLVKQGIKSDFTNISLSVQLNTFKQIL 154

Query: 122 NSICYNQ-RDCNEKLHNTLFLVCEIGGNDYNYALFQSKTIQEVEDMVP-DVQSIKAAVER 181
            ++C +  RDC E L ++L L+ EIGGNDYNY  F+ K+I E++++VP  +++I +A+  
Sbjct: 155 PNLCASSTRDCREMLGDSLILMGEIGGNDYNYPFFEGKSINEIKELVPLIIKAISSAIVD 214

Query: 182 VISYGTTRVVVPGNFPIGCFSIYLTGFQTNDTTACDELHCLKDLNNFASYHNDQIKEMIE 241
           +I  G    +VPGNFPIGC + YLT FQT          C+  LN F  +HN+Q+K  ++
Sbjct: 215 LIDLGGKTFLVPGNFPIGCSTAYLTLFQTATVEHDPFTGCIPWLNKFGEHHNEQLKIELK 274

Query: 242 ALKKENPHTVIVYGDYYNAFLWIIRRAFILGMFFTPHK 274
            L+K  PH  I+Y DYYN+         + G+F  P K
Sbjct: 275 QLQKLYPHVNIIYADYYNS---------LYGLFQEPAK 303

[Close]

BLAST of Lsi03G006760 vs. NCBI nr
Match: gi|778730277|ref|XP_011659746.1| (PREDICTED: acetylajmalan esterase-like [Cucumis sativus])

HSP 1 Score: 447.2 bits (1149), Expect = 2.1e-122
Identity = 217/268 (80.97%), Postives = 239/268 (89.18%), Query Frame = 1


Query: 1   MFDAIYQLGNSIFDTNNLIRENLNTTFSHLPYGETFFNNPTGRCSNGLLMIDYFALDAGL 60
           MFDAIYQLG+SI DT NLIREN NT FSHLPYG++FFNNPTGRCSNGLLM+D+ ALDAGL
Sbjct: 32  MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDFLALDAGL 91

Query: 61  PLVNPYLNKDALTNHGVNFAMAGSTALSSKLLSQK-TISSPVTNSSLNRQLDWMFSHFNS 120
           PLV+PYLNKD L +HGVNFA+AGSTAL S+ LS    I SPVTNSSL+ QLDWMFSHFNS
Sbjct: 92  PLVSPYLNKDGLMDHGVNFAVAGSTALPSQYLSSSYKIISPVTNSSLDHQLDWMFSHFNS 151

Query: 121 ICYNQRDCNEKLHNTLFLVCEIGGNDYNYALFQSKTIQEVEDMVPD-VQSIKAAVERVIS 180
           IC+NQR+CNEKL + LFLV EIGGNDYNYALFQ KTIQE +DMVPD VQ+IK+AVE+VIS
Sbjct: 152 ICHNQRECNEKLRSALFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVIS 211

Query: 181 YGTTRVVVPGNFPIGCFSIYLTGFQTNDTTACDELHCLKDLNNFASYHNDQIKEMIEALK 240
           YG TRVVVPGNFPIGCF IYLTGF TNDT+A DELHCLKDLN+FA+YHNDQIK+ IE LK
Sbjct: 212 YGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNSFATYHNDQIKQAIEVLK 271

Query: 241 KENPHTVIVYGDYYNAFLWIIRRAFILG 267
           KENPH VIVYGDYYNAFLWIIR AF+LG
Sbjct: 272 KENPHAVIVYGDYYNAFLWIIRHAFVLG 299

[Close]

BLAST of Lsi03G006760 vs. NCBI nr
Match: gi|659131079|ref|XP_008465497.1| (PREDICTED: acetylajmalan esterase-like, partial [Cucumis melo])

HSP 1 Score: 423.3 bits (1087), Expect = 3.2e-115
Identity = 210/267 (78.65%), Postives = 231/267 (86.52%), Query Frame = 1


Query: 2   FDAIYQLGNSIFDTNNLIRENLNTTFSHLPYGETFFNN-PTGRCSNGLLMIDYFALDAGL 61
           FD IYQLG+SI DT NLIREN NT FSHLPYG+TFFN+ PTGRCSNGLL+IDYFALDA L
Sbjct: 19  FDGIYQLGDSISDTGNLIRENPNTPFSHLPYGQTFFNSTPTGRCSNGLLIIDYFALDAKL 78

Query: 62  PLVNPYLNKDALTNHGVNFAMAGSTALSSKLLSQKTISSPVTNSSLNRQLDWMFSHFNSI 121
           PLVNPYLNKDALT HGVNFA+AGSTALSS+LLS+K ISSP+TNSSL+ QLDWMFSHFNSI
Sbjct: 79  PLVNPYLNKDALTRHGVNFAVAGSTALSSQLLSEKKISSPLTNSSLDIQLDWMFSHFNSI 138

Query: 122 CYNQRDCNEKLHNTLFLVCEIGGNDYNYALFQSKTIQEVEDMVPD-VQSIKAAVERVISY 181
           C++Q+DCNEKL N LFLV EIG NDYNYAL Q KTIQEV++MVP+ VQ++K AVERVISY
Sbjct: 139 CFDQKDCNEKLMNALFLVGEIGANDYNYALLQGKTIQEVKEMVPEVVQTVKNAVERVISY 198

Query: 182 GTTRVVVPGNFPIGCFSIYLTGFQTNDTTACDELHCLKDLNNFASYHNDQIKEMIEALKK 241
           G TRVVV GNFPIGCF I+LT FQTNDTTA DE HCLK LN   SYHNDQI++ IE L K
Sbjct: 199 GATRVVVSGNFPIGCFPIFLTVFQTNDTTAYDEFHCLKSLNALTSYHNDQIQQAIEVLNK 258

Query: 242 ENPHTVIVYGDYYNAFLWIIRRAFILG 267
           EN  TVIVYGDYYNAFLWI+R A  LG
Sbjct: 259 ENSRTVIVYGDYYNAFLWILRHASTLG 285

[Close]

BLAST of Lsi03G006760 vs. NCBI nr
Match: gi|778674018|ref|XP_004144819.2| (PREDICTED: acetylajmalan esterase-like [Cucumis sativus])

HSP 1 Score: 417.5 bits (1072), Expect = 1.8e-113
Identity = 211/270 (78.15%), Postives = 234/270 (86.67%), Query Frame = 1


Query: 2   FDAIYQLGNSIFDTNNLIRENLNTTFSHLPYGETFFNN-PTGRCSNGLLMIDYFALDAGL 61
           FD IYQLG+SI DT NLIREN NT FSHLPYG+TFFN+ PTGRCSNGLL+IDYFALDA L
Sbjct: 33  FDGIYQLGDSISDTGNLIRENPNTPFSHLPYGQTFFNSTPTGRCSNGLLIIDYFALDARL 92

Query: 62  PLVNPYLNKDALTNHGVNFAMAGSTALSSKLLSQKTISSPVTNSSLNRQLDWMFSHFNSI 121
           PLVNPYLNKDALT HG+NFA+AGSTALSS+LLS+K ISS +TNSSL+ QLDWMFSHFNSI
Sbjct: 93  PLVNPYLNKDALTRHGINFAVAGSTALSSELLSKKKISSLLTNSSLDLQLDWMFSHFNSI 152

Query: 122 CYNQRDCNEKLHNTLFLVCEIGGNDYNYALFQSKTIQEVEDMVPD-VQSIKAAVERVISY 181
           CY+Q+DC+EKL N LFLV EIG NDYNYAL Q KTI+EV++MVP+ VQ+IK AVERVISY
Sbjct: 153 CYDQKDCDEKLKNALFLVGEIGANDYNYALLQGKTIEEVKEMVPEVVQAIKNAVERVISY 212

Query: 182 GTTRVVVPGNFPIGCFSIYLTGFQTNDTT---ACDELHCLKDLNNFASYHNDQIKEMIEA 241
           G TRVVV GNFPIGCF I LT FQTN+TT     DE HCLK LN  ASYHNDQIK++IE 
Sbjct: 213 GATRVVVSGNFPIGCFPISLTVFQTNNTTDYDEYDEYHCLKSLNALASYHNDQIKQVIEV 272

Query: 242 LKKENPHTVIVYGDYYNAFLWIIRRAFILG 267
           LKKEN HTVIVYGDYYNAFLWI+RRA +LG
Sbjct: 273 LKKENLHTVIVYGDYYNAFLWILRRASMLG 302

[Close]

BLAST of Lsi03G006760 vs. NCBI nr
Match: gi|449469178|ref|XP_004152298.1| (PREDICTED: GDSL esterase/lipase At5g03980-like [Cucumis sativus])

HSP 1 Score: 361.3 bits (926), Expect = 1.5e-96
Identity = 171/266 (64.29%), Postives = 212/266 (79.70%), Query Frame = 1


Query: 3   DAIYQLGNSIFDTNNLIRENLNTTFSHLPYGETFFNNPTGRCSNGLLMIDYFALDAGLPL 62
           D+I+Q G+S+ DT NLIREN +T FSHLPYG+TFFN PTGRCSNGLLM+DYFAL AGLPL
Sbjct: 39  DSIFQFGDSLADTGNLIRENPSTPFSHLPYGQTFFNKPTGRCSNGLLMVDYFALAAGLPL 98

Query: 63  VNPYLNKDALTNHGVNFAMAGSTALSSKLLSQKTISSPVTNSSLNRQLDWMFSHFNSICY 122
           VNPYL K A   HGVNFA+AGSTAL   +L+Q  I+SPVTN+SL++QLDWM S+ N+IC 
Sbjct: 99  VNPYLQKKASFVHGVNFAVAGSTALPLDVLAQNNITSPVTNTSLSKQLDWMHSYLNTICS 158

Query: 123 NQR-DCNEKLHNTLFLVCEIGGNDYNYALFQSKTIQEVEDMVPD-VQSIKAAVERVISYG 182
           N+R DC  KL + LF + EIGGNDYNYALF+ KT+ EV++MVP  VQ+I  A +RVI YG
Sbjct: 159 NKRDDCTTKLKHALFFMGEIGGNDYNYALFEGKTVAEVKNMVPRVVQTIMDATKRVIDYG 218

Query: 183 TTRVVVPGNFPIGCFSIYLTGFQTNDTTACDELHCLKDLNNFASYHNDQIKEMIEALKKE 242
            TRV++PG+F +GC  IYLTGFQTND+TA D+ HCLKD N  ASYHN ++K+ I+ L+KE
Sbjct: 219 ATRVIIPGHFSMGCLPIYLTGFQTNDSTAYDKFHCLKDFNGLASYHNKKLKQAIKLLRKE 278

Query: 243 NPHTVIVYGDYYNAFLWIIRRAFILG 267
           NP+ +I YGDYYNA  W+ + A +LG
Sbjct: 279 NPNVIIAYGDYYNALFWVFQHASLLG 304

[Close]

BLAST of Lsi03G006760 vs. NCBI nr
Match: gi|659108979|ref|XP_008454485.1| (PREDICTED: GDSL esterase/lipase At5g03980-like [Cucumis melo])

HSP 1 Score: 358.6 bits (919), Expect = 9.8e-96
Identity = 168/266 (63.16%), Postives = 211/266 (79.32%), Query Frame = 1


Query: 3   DAIYQLGNSIFDTNNLIRENLNTTFSHLPYGETFFNNPTGRCSNGLLMIDYFALDAGLPL 62
           D+I+Q G+S+ DT NLIREN +T F+HLPYG+TFFN PTGRCSNGLLM+DYFAL AGLPL
Sbjct: 43  DSIFQFGDSLADTGNLIRENPSTPFAHLPYGQTFFNKPTGRCSNGLLMVDYFALAAGLPL 102

Query: 63  VNPYLNKDALTNHGVNFAMAGSTALSSKLLSQKTISSPVTNSSLNRQLDWMFSHFNSICY 122
           VNPYL K A   HGVNFA+AGSTAL   +L+Q  ++SPVTN+SL +QLDWM S+  +IC 
Sbjct: 103 VNPYLQKKASFVHGVNFAVAGSTALPLDVLAQNNVTSPVTNTSLTKQLDWMHSYLRTICS 162

Query: 123 NQR-DCNEKLHNTLFLVCEIGGNDYNYALFQSKTIQEVEDMVPD-VQSIKAAVERVISYG 182
           N+R DC  KL + LF + EIGGNDYNYALF+ KT+ EV++MVP  VQ+I  A +RVI YG
Sbjct: 163 NKRDDCTMKLKHALFFMGEIGGNDYNYALFEGKTVSEVKNMVPRVVQTIMGATKRVIDYG 222

Query: 183 TTRVVVPGNFPIGCFSIYLTGFQTNDTTACDELHCLKDLNNFASYHNDQIKEMIEALKKE 242
            TRV++PG+F +GC  IYLTGFQTND+TA D+ HCLKD N  ASYHN+++K+ I+ L+KE
Sbjct: 223 ATRVIIPGHFSMGCLPIYLTGFQTNDSTAYDKFHCLKDFNGLASYHNNKLKQAIKLLRKE 282

Query: 243 NPHTVIVYGDYYNAFLWIIRRAFILG 267
           NP+ +I YGDYYNA  W+ + A +LG
Sbjct: 283 NPNVIIAYGDYYNALFWVFQHASLLG 308

The following BLAST results are available for this feature:

BLAST of Lsi03G006760 vs. Swiss-Prot


Match Name	E-value	Identity	Description
AAE_RAUSE	2.6e-56	43.12	Acetylajmalan esterase OS=Rauvolfia serpentina GN=AAE PE=1 SV=1	[more]
GDL8_ARATH	7.8e-53	42.75	GDSL esterase/lipase At1g28590 OS=Arabidopsis thaliana GN=At1g28590 PE=2 SV=2	[more]
GDL6_ARATH	7.8e-53	41.67	GDSL esterase/lipase At1g28570 OS=Arabidopsis thaliana GN=At1g28570 PE=2 SV=1	[more]
GDL7_ARATH	2.5e-51	41.67	GDSL esterase/lipase At1g28580 OS=Arabidopsis thaliana GN=At1g28580 PE=2 SV=1	[more]
GDL9_ARATH	3.6e-50	41.52	GDSL esterase/lipase At1g28600 OS=Arabidopsis thaliana GN=At1g28600 PE=2 SV=1	[more]

BLAST of Lsi03G006760 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A061FGU1_THECC	1.0e-91	62.31	SGNH hydrolase-type esterase superfamily protein, putative OS=Theobroma cacao GN...	[more]
A0A061F1T9_THECC	2.3e-91	61.94	SGNH hydrolase-type esterase superfamily protein, putative OS=Theobroma cacao GN...	[more]
V4V456_9ROSI	3.3e-90	61.30	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10003375mg PE=4 SV=1	[more]
A0A061EYZ6_THECC	9.5e-90	61.80	SGNH hydrolase-type esterase superfamily protein, putative OS=Theobroma cacao GN...	[more]
U5G9P4_POPTR	6.2e-89	61.94	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0005s02420g PE=4 SV=1	[more]

BLAST of Lsi03G006760 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G28570.1	4.4e-54	41.67	SGNH hydrolase-type esterase superfamily protein	[more]
AT1G28590.1	4.4e-54	42.75	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT1G28580.1	1.4e-52	41.67	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT1G28600.1	2.0e-51	41.52	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT1G28650.1	1.3e-50	40.65	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]

BLAST of Lsi03G006760 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778730277\|ref\|XP_011659746.1\|	2.1e-122	80.97	PREDICTED: acetylajmalan esterase-like [Cucumis sativus]	[more]
gi\|659131079\|ref\|XP_008465497.1\|	3.2e-115	78.65	PREDICTED: acetylajmalan esterase-like, partial [Cucumis melo]	[more]
gi\|778674018\|ref\|XP_004144819.2\|	1.8e-113	78.15	PREDICTED: acetylajmalan esterase-like [Cucumis sativus]	[more]
gi\|449469178\|ref\|XP_004152298.1\|	1.5e-96	64.29	PREDICTED: GDSL esterase/lipase At5g03980-like [Cucumis sativus]	[more]
gi\|659108979\|ref\|XP_008454485.1\|	9.8e-96	63.16	PREDICTED: GDSL esterase/lipase At5g03980-like [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016788	hydrolase activity, acting on ester bonds

Vocabulary: **INTERPRO**
Term	Definition
IPR001087	GDSL

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G006760.1	Lsi03G006760.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001087	GDSL lipase/esterase	PFAM	PF00657	Lipase_GDSL	coord: 98..258 score: 2.
None	No IPR available	PANTHER	PTHR22835	ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN	coord: 2..267 score: 4.2E
None	No IPR available	PANTHER	PTHR22835:SF133	SUBFAMILY NOT NAMED	coord: 2..267 score: 4.2E

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi03G006760	Cucumber (Gy14) v1	cgylsiB367
Lsi03G006760	Cucurbita maxima (Rimu)	cmalsiB484
Lsi03G006760	Cucurbita moschata (Rifu)	cmolsiB468
Lsi03G006760	Cucurbita pepo (Zucchini)	cpelsiB208
Lsi03G006760	Wild cucumber (PI 183967)	cpilsiB536
Lsi03G006760	Cucumber (Chinese Long) v2	culsiB522
Lsi03G006760	Melon (DHL92) v3.5.1	lsimeB247
Lsi03G006760	Watermelon (Charleston Gray)	lsiwcgB212
Lsi03G006760	Watermelon (Charleston Gray)	lsiwcgB215
Lsi03G006760	Watermelon (97103) v1	lsiwmB227
Lsi03G006760	Watermelon (97103) v1	lsiwmB233
Lsi03G006760	Cucumber (Gy14) v2	cgyblsiB490
Lsi03G006760	Melon (DHL92) v3.6.1	lsimedB260
Lsi03G006760	Silver-seed gourd	carlsiB262
Lsi03G006760	Silver-seed gourd	carlsiB671
Lsi03G006760	Cucumber (Chinese Long) v3	cuclsiB555
Lsi03G006760	Watermelon (97103) v2	lsiwmbB247
Lsi03G006760	Watermelon (97103) v2	lsiwmbB251
Lsi03G006760	Wax gourd	lsiwgoB320