Lsi03G006480 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi03G006480
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Subtilase family protein
Location	chr03 : 8183360 .. 8184730 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAAACAAAAGCAATTCTTCTTAATTTTGTACAAAATTGTGACTCCATCTCTATTCCTAATTCCTATAGTGTTGAAGTTGAAAGAATGGCAAATACAAAGATGAATTTTTCCTCATTTACCTTGTATCTTCTTTTATTGTTGACAATTTCTCTGAATTTGCTTCTTAGCTCGACGACAACATCAGTAAGATCCACCACCTCCGAGATTGGTTTACTATCGTCATTGCATATCGTTTACACTTCTGAGAGGCCTAGCAATGAGAAACCTGAGGCCTTTAACCTCCAAATCCTAACTTCTGTTCTCGGCAGGTATGTTTTTCTTGAAAAATTATTCAAAGAGACTGCCACATTATTTTTAGACGAAAAATTATTCAATATGGACATCACAAAGTGGTGTGCAATTTTACACCATTGGATGTGGATTCTAAAATTTGCATGCTCATAATAACCACATGTTCTTTTACCGTTCTCTCTCATACATCCAACACTACAAAATTGTCGGCCGTATTATGGAATATTATCGTTGCTCGCAATGGTTAATTGTTAGGAACATCCACATTGAGATATGTTCATTGAATAATTTTTCGTTTTCTAAATACGTCTCGTGTGTGTCGTCTTTTCTCTGACCAAATGATGGGTTAATTGAAGGTGAATTTTTGTGTCAATTATTTGTTGGTTTTATTCCAGTAAAGAAGCTGCTAAGAAAGCTCTTGTGTATAGTTTTAATAATTCTATGACTGGCTTTGCTGCTAATCTGACTCCTGATCAGGTTAAGAAAATTTCAGGTAACTATTGTTCTAAATATTATGTTTTTTTTTTTTAATAATAACTTAGGATGTGATTAGATCTTCACAACTTTTTATTTTGGGATTTTGGCAGAACACCCCGGAGTTGTACATGTTGTCCAAAGCGTTAATTATAATTTGCAGACAGAAGGAAAAAGGGATAGTAAAAATGTATAGAAGAGTTTCAATATCTTTCTCTTCAAATATGGGTTTGTTGCTTAAATAAAAGGCGCAATTGCAAGTATAGCAATCATGCCCAAAATATTAGCAAATATAATACAATGCAAAAGAATTTACAAATATAACAAAATTTAGATCTAGCTCTTAGAGTTTATCGGTGATAAACTATATCGGTGATAGGACGAGTCTATTAGCGATAGAGTCTATCACTAATAGGAATTTATCATCGAATGAATCTATAATTGATAGATTTTGCTATATTTGTAATTCCTTAAAAATGTTGTTATACATTTAATTGTTAGTCCTAAATTGATACCCATTGTAATAACCGTGTTATTCATTGCAATTACCCTAAATAAAATGTATTTGGTGTTAAACCATGGTTTAAGGAGTTTTTATATCCCTT

mRNA sequence

CAAAACAAAAGCAATTCTTCTTAATTTTGTACAAAATTGTGACTCCATCTCTATTCCTAATTCCTATAGTGTTGAAGTTGAAAGAATGGCAAATACAAAGATGAATTTTTCCTCATTTACCTTGTATCTTCTTTTATTGTTGACAATTTCTCTGAATTTGCTTCTTAGCTCGACGACAACATCAGTAAGATCCACCACCTCCGAGATTGGTTTACTATCGTCATTGCATATCGTTTACACTTCTGAGAGGCCTAGCAATGAGAAACCTGAGGCCTTTAACCTCCAAATCCTAACTTCTGTTCTCGGCAGTAAAGAAGCTGCTAAGAAAGCTCTTGTGTATAGTTTTAATAATTCTATGACTGGCTTTGCTGCTAATCTGACTCCTGATCAGGTTAAGAAAATTTCAGAACACCCCGGAGTTGTACATGTTGTCCAAAGCGTTAATTATAATTTGCAGACAGAAGGAAAAAGGGATAGTAAAAATGTATAGAAGAGTTTCAATATCTTTCTCTTCAAATATGGGTTTGTTGCTTAAATAAAAGGCGCAATTGCAAGTATAGCAATCATGCCCAAAATATTAGCAAATATAATACAATGCAAAAGAATTTACAAATATAACAAAATTTAGATCTAGCTCTTAGAGTTTATCGGTGATAAACTATATCGGTGATAGGACGAGTCTATTAGCGATAGAGTCTATCACTAATAGGAATTTATCATCGAATGAATCTATAATTGATAGATTTTGCTATATTTGTAATTCCTTAAAAATGTTGTTATACATTTAATTGTTAGTCCTAAATTGATACCCATTGTAATAACCGTGTTATTCATTGCAATTACCCTAAATAAAATGTATTTGGTGTTAAACCATGGTTTAAGGAGTTTTTATATCCCTT

Coding sequence (CDS)

ATGGCAAATACAAAGATGAATTTTTCCTCATTTACCTTGTATCTTCTTTTATTGTTGACAATTTCTCTGAATTTGCTTCTTAGCTCGACGACAACATCAGTAAGATCCACCACCTCCGAGATTGGTTTACTATCGTCATTGCATATCGTTTACACTTCTGAGAGGCCTAGCAATGAGAAACCTGAGGCCTTTAACCTCCAAATCCTAACTTCTGTTCTCGGCAGTAAAGAAGCTGCTAAGAAAGCTCTTGTGTATAGTTTTAATAATTCTATGACTGGCTTTGCTGCTAATCTGACTCCTGATCAGGTTAAGAAAATTTCAGAACACCCCGGAGTTGTACATGTTGTCCAAAGCGTTAATTATAATTTGCAGACAGAAGGAAAAAGGGATAGTAAAAATGTATAG

Protein sequence

MANTKMNFSSFTLYLLLLLTISLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEKPEAFNLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVNYNLQTEGKRDSKNV

Homology

[Close]

BLAST of Lsi03G006480 vs. Swiss-Prot
Match: SBT3B_ARATH (Subtilisin-like protease SBT3.11 OS=Arabidopsis thaliana GN=SBT3.11 PE=2 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 7.2e-12
Identity = 38/84 (45.24%), Postives = 58/84 (69.05%), Query Frame = 1


Query: 45  SSLHIVYTSERPSNEKPE---AFNLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPD 104
           + +HIVY  E+  N+ PE   + +L++L S+LGSK+ A +++V+S+ N  +GFAA+LT  
Sbjct: 35  TKVHIVYLGEKEHND-PELVTSSHLRMLESLLGSKKDASESIVHSYRNGFSGFAAHLTDS 94

Query: 105 QVKKISEHPGVVHVVQSVNYNLQT 126
           Q ++ISEHP VV V  +  Y LQT
Sbjct: 95  QAEQISEHPDVVQVTPNTFYELQT 117

[Close]

BLAST of Lsi03G006480 vs. Swiss-Prot
Match: SBT3D_ARATH (Subtilisin-like protease SBT3.13 OS=Arabidopsis thaliana GN=SBT3.13 PE=2 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 4.0e-10
Identity = 51/123 (41.46%), Postives = 74/123 (60.16%), Query Frame = 1


Query: 6   MNFSSFTLYLLLLLTISLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEKPE--- 65
           MN S  +  L+LLL I+L L L+ T     +    +   S ++IVY  ER  ++ PE   
Sbjct: 1   MNNSLQSSKLVLLLAIALVLFLN-TELDFLTAAGALDSDSKVYIVYLGER-EHDDPELVT 60

Query: 66  AFNLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVNYN 125
           A + Q+L S+L SKE A+ +L+YS+ +  +GFAA LT  Q KKISEHP V+HV+ +    
Sbjct: 61  ASHHQMLESLLQSKEDAQNSLIYSYQHGFSGFAALLTSSQAKKISEHPEVIHVIPNRIRK 120

[Close]

BLAST of Lsi03G006480 vs. Swiss-Prot
Match: SBT36_ARATH (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana GN=SBT3.6 PE=2 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.5e-09
Identity = 47/123 (38.21%), Postives = 71/123 (57.72%), Query Frame = 1


Query: 6   MNFSSFTLYLLLLLTISLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEKPEAF- 65
           MN+ + ++Y++L L I LN+         RS  +E      +HIVY  E+  ++ PE   
Sbjct: 2   MNYRT-SIYVVLSLVIFLNVQ--------RSFVAESSAKRKVHIVYLGEK-QHDDPEFVT 61

Query: 66  --NLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVNYN 125
             + ++L S+LGSKE A  ++VYS+ +  +GFAA LT  Q KKI++ P VVHV+    Y 
Sbjct: 62  ESHHRMLWSLLGSKEDANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYK 114

[Close]

BLAST of Lsi03G006480 vs. Swiss-Prot
Match: SBT33_ARATH (Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana GN=SBT3.3 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 2.0e-09
Identity = 42/107 (39.25%), Postives = 64/107 (59.81%), Query Frame = 1


Query: 22  SLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEKPEAF---NLQILTSVLGSKEA 81
           S+ L+L S  T + +T +     S +HIVY  E+  ++ PE     + Q+L S+LGSK+ 
Sbjct: 7   SILLVLLSLITVLNATRARSETESKVHIVYLGEKKHHD-PEFVTESHHQMLASLLGSKKD 66

Query: 82  AKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVNYNLQT 126
           A  ++VYS+ +  +GFAA LT  Q KKI++ P VVHV+    + L T
Sbjct: 67  ADDSMVYSYRHGFSGFAAKLTKSQAKKIADLPEVVHVIPDGFHELAT 112

[Close]

BLAST of Lsi03G006480 vs. Swiss-Prot
Match: SBT34_ARATH (Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana GN=SBT3.4 PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 4.4e-09
Identity = 39/111 (35.14%), Postives = 65/111 (58.56%), Query Frame = 1


Query: 17  LLLTISLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEKP--EAFNLQILTSVLG 76
           +L+ +SL ++L+    S +S          +HIVY  E+  ++       + Q+L+S+LG
Sbjct: 8   VLVVLSLIIVLNVARASAKS---------KVHIVYLGEKQHDDPKFVTESHHQMLSSLLG 67

Query: 77  SKEAAKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVNYNLQT 126
           SK+ A +++VYS+ +  +GFAA LT  Q KKI++ P V+HV+    Y L T
Sbjct: 68  SKDDAHESMVYSYRHGFSGFAAKLTKSQAKKIADSPEVIHVIPDSYYELAT 109

[Close]

BLAST of Lsi03G006480 vs. TrEMBL
Match: A0A0A0K630_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G274680 PE=4 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 2.3e-33
Identity = 85/131 (64.89%), Postives = 104/131 (79.39%), Query Frame = 1


Query: 1   MANTKMNFSSFTLYLLLLLTISLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEK 60
           M NTKMNFSSFTL L+LL TISLN  L S   S +   S+  + SS+++VYT++RPSNEK
Sbjct: 1   MTNTKMNFSSFTLSLVLLFTISLNSHLISGMISDKPV-SKFVVPSSIYMVYTAKRPSNEK 60

Query: 61  PEAFNLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVN 120
           PE F +QIL SV+ S EAAKKALVYSF +S+ GFAANLTP+QVKKI   PGV+HV +SVN
Sbjct: 61  PETFYIQILASVIDSNEAAKKALVYSFKSSINGFAANLTPNQVKKILAQPGVLHVARSVN 120

Query: 121 YNLQTEGKRDS 132
           YNLQTEGK+++
Sbjct: 121 YNLQTEGKKNN 130

[Close]

BLAST of Lsi03G006480 vs. TrEMBL
Match: E5GCJ1_CUCME (Peptidase OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 2.0e-29
Identity = 75/113 (66.37%), Postives = 91/113 (80.53%), Query Frame = 1


Query: 19  LTISLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEKPEAFNLQILTSVLGSKEA 78
           +++SLN LL+    S +S  S+  + SSLHIVYT++RPSNEKPEAF +QIL SVLGS EA
Sbjct: 19  VSVSLNSLLAWGMISEKSM-SKFAVPSSLHIVYTAKRPSNEKPEAFYIQILASVLGSNEA 78

Query: 79  AKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVNYNLQTEGKRDS 132
           A+KALVYSF NSM GFAANLTP+QVKKIS  PGV+HV +SV YNLQT GK ++
Sbjct: 79  ARKALVYSFKNSMNGFAANLTPNQVKKISAQPGVLHVARSVTYNLQTGGKENN 130

[Close]

BLAST of Lsi03G006480 vs. TrEMBL
Match: A0A0A0K6B0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G291690 PE=4 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.3e-20
Identity = 66/124 (53.23%), Postives = 91/124 (73.39%), Query Frame = 1


Query: 9   SSFTLYLLLLLTISLNLLLSSTTTSVRSTTSEIGLLSS-----LHIVYTSERPSNEKPEA 68
           ++  + LL L +ISLN  LSST  S  S ++ I L+SS     +HIVYT ERP NE+PEA
Sbjct: 2   ANLNISLLFLFSISLNFHLSSTAMS--SQSASIPLVSSFDSPAVHIVYT-ERPHNEEPEA 61

Query: 69  FNLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVNYNL 128
           ++++ L SVLGS+EAA++ALVYS+ N+ +GF+A LTPDQV +I++ PGV+HVVQS N  L
Sbjct: 62  YHIRTLVSVLGSEEAAREALVYSYKNAASGFSARLTPDQVTEITKQPGVLHVVQSGNNKL 121

[Close]

BLAST of Lsi03G006480 vs. TrEMBL
Match: E5GCJ0_CUCME (Putative peptidase OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 2.6e-16
Identity = 46/86 (53.49%), Postives = 68/86 (79.07%), Query Frame = 1


Query: 42  GLLSSLHIVYTSERPSNEKPEAFNLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPD 101
           G  +++HIVY +ERP NE+PEA++++ L SVLGS++AA++ALVYS+ N+ +GF+A LT D
Sbjct: 3   GQSTAVHIVY-TERPHNEEPEAYHIRTLASVLGSQDAAREALVYSYKNAASGFSARLTSD 62

Query: 102 QVKKISEHPGVVHVVQSVNYNLQTEG 128
           QV +I++ PGV+ VVQS N  L + G
Sbjct: 63  QVAEIAKQPGVLQVVQSGNNKLHSGG 87

[Close]

BLAST of Lsi03G006480 vs. TrEMBL
Match: A5BSE8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0117g00130 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 7.5e-16
Identity = 45/81 (55.56%), Postives = 65/81 (80.25%), Query Frame = 1


Query: 45  SSLHIVYTSERPSNEKPEAFNLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPDQVK 104
           +++HI+Y +E+P NE+PEAF+L+ L+SVLGS+EAAKKAL+YS+ N+ +GF+A LTP+QV 
Sbjct: 14  AAVHIIY-TEKPLNEEPEAFHLRTLSSVLGSEEAAKKALIYSYKNAASGFSAKLTPEQVS 73

Query: 105 KISEHPGVVHVVQSVNYNLQT 126
           +IS  PGV+ VV S    L +
Sbjct: 74  QISTLPGVLQVVPSRTLQLHS 93

[Close]

BLAST of Lsi03G006480 vs. TAIR10
Match: AT1G71950.1 (AT1G71950.1 Proteinase inhibitor, propeptide)

HSP 1 Score: 80.1 bits (196), Expect = 1.1e-15
Identity = 44/114 (38.60%), Postives = 76/114 (66.67%), Query Frame = 1


Query: 14  YLLLLLTISLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEKPEAFNLQILTSVL 73
           +   L +   +++++   T   + +SE    + +HI+Y +E+P++E+P+ ++L+ L+S L
Sbjct: 20  FFFCLSSFFASIVMADEYTGEATGSSE----AKVHIIY-TEKPTDEEPKTYHLRTLSSAL 79

Query: 74  GSKEAAKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVNYNLQTEG 128
           GS+EAAK AL+YS+  + +GF+A LTP+QV +IS+ PGV+ VV S  Y L   G
Sbjct: 80  GSEEAAKDALIYSYKEAASGFSAKLTPEQVAEISKQPGVIQVVPSQTYQLHKPG 128

[Close]

BLAST of Lsi03G006480 vs. TAIR10
Match: AT5G11940.1 (AT5G11940.1 Subtilase family protein)

HSP 1 Score: 71.6 bits (174), Expect = 4.1e-13
Identity = 38/84 (45.24%), Postives = 58/84 (69.05%), Query Frame = 1


Query: 45  SSLHIVYTSERPSNEKPE---AFNLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPD 104
           + +HIVY  E+  N+ PE   + +L++L S+LGSK+ A +++V+S+ N  +GFAA+LT  
Sbjct: 35  TKVHIVYLGEKEHND-PELVTSSHLRMLESLLGSKKDASESIVHSYRNGFSGFAAHLTDS 94

Query: 105 QVKKISEHPGVVHVVQSVNYNLQT 126
           Q ++ISEHP VV V  +  Y LQT
Sbjct: 95  QAEQISEHPDVVQVTPNTFYELQT 117

[Close]

BLAST of Lsi03G006480 vs. TAIR10
Match: AT4G21650.1 (AT4G21650.1 Subtilase family protein)

HSP 1 Score: 65.9 bits (159), Expect = 2.2e-11
Identity = 51/123 (41.46%), Postives = 74/123 (60.16%), Query Frame = 1


Query: 6   MNFSSFTLYLLLLLTISLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEKPE--- 65
           MN S  +  L+LLL I+L L L+ T     +    +   S ++IVY  ER  ++ PE   
Sbjct: 1   MNNSLQSSKLVLLLAIALVLFLN-TELDFLTAAGALDSDSKVYIVYLGER-EHDDPELVT 60

Query: 66  AFNLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVNYN 125
           A + Q+L S+L SKE A+ +L+YS+ +  +GFAA LT  Q KKISEHP V+HV+ +    
Sbjct: 61  ASHHQMLESLLQSKEDAQNSLIYSYQHGFSGFAALLTSSQAKKISEHPEVIHVIPNRIRK 120

[Close]

BLAST of Lsi03G006480 vs. TAIR10
Match: AT4G10550.3 (AT4G10550.3 Subtilase family protein)

HSP 1 Score: 65.9 bits (159), Expect = 2.2e-11
Identity = 40/97 (41.24%), Postives = 57/97 (58.76%), Query Frame = 1


Query: 32  TSVRSTTSEIGLLSSLHIVYTSERPSNEKPEAF---NLQILTSVLGSKEAAKKALVYSFN 91
           T  RS  +E      +HIVY  E+  ++ PE     + ++L S+LGSKE A  ++VYS+ 
Sbjct: 35  TKERSFVAESSAKRKVHIVYLGEK-QHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYR 94

Query: 92  NSMTGFAANLTPDQVKKISEHPGVVHVVQSVNYNLQT 126
           +  +GFAA LT  Q KKI++ P VVHV+    Y L T
Sbjct: 95  HGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLAT 130

[Close]

BLAST of Lsi03G006480 vs. TAIR10
Match: AT1G32960.1 (AT1G32960.1 Subtilase family protein)

HSP 1 Score: 63.5 bits (153), Expect = 1.1e-10
Identity = 42/107 (39.25%), Postives = 64/107 (59.81%), Query Frame = 1


Query: 22  SLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEKPEAF---NLQILTSVLGSKEA 81
           S+ L+L S  T + +T +     S +HIVY  E+  ++ PE     + Q+L S+LGSK+ 
Sbjct: 7   SILLVLLSLITVLNATRARSETESKVHIVYLGEKKHHD-PEFVTESHHQMLASLLGSKKD 66

Query: 82  AKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVNYNLQT 126
           A  ++VYS+ +  +GFAA LT  Q KKI++ P VVHV+    + L T
Sbjct: 67  ADDSMVYSYRHGFSGFAAKLTKSQAKKIADLPEVVHVIPDGFHELAT 112

[Close]

BLAST of Lsi03G006480 vs. NCBI nr
Match: gi|659124047|ref|XP_008461963.1| (PREDICTED: xylem serine proteinase 1-like [Cucumis melo])

HSP 1 Score: 159.8 bits (403), Expect = 3.2e-36
Identity = 90/131 (68.70%), Postives = 105/131 (80.15%), Query Frame = 1


Query: 1   MANTKMNFSSFTLYLLLLLTISLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEK 60
           M NTKMNFSS  LYL+LL T+SLN LL+    S +S  S+  + SSLHIVYT++RPSNEK
Sbjct: 1   MTNTKMNFSSSPLYLVLLFTVSLNSLLAWGMISEKSM-SKFAVPSSLHIVYTAKRPSNEK 60

Query: 61  PEAFNLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVN 120
           PEAF +QIL SVLGS EAA+KALVYSF NSM GFAANLTP+QVKKIS  PGV+HV +SV 
Sbjct: 61  PEAFYIQILASVLGSNEAARKALVYSFKNSMNGFAANLTPNQVKKISAQPGVLHVARSVT 120

Query: 121 YNLQTEGKRDS 132
           YNLQT GK ++
Sbjct: 121 YNLQTGGKENN 130

[Close]

BLAST of Lsi03G006480 vs. NCBI nr
Match: gi|700189146|gb|KGN44379.1| (hypothetical protein Csa_7G274680 [Cucumis sativus])

HSP 1 Score: 149.8 bits (377), Expect = 3.3e-33
Identity = 85/131 (64.89%), Postives = 104/131 (79.39%), Query Frame = 1


Query: 1   MANTKMNFSSFTLYLLLLLTISLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEK 60
           M NTKMNFSSFTL L+LL TISLN  L S   S +   S+  + SS+++VYT++RPSNEK
Sbjct: 1   MTNTKMNFSSFTLSLVLLFTISLNSHLISGMISDKPV-SKFVVPSSIYMVYTAKRPSNEK 60

Query: 61  PEAFNLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVN 120
           PE F +QIL SV+ S EAAKKALVYSF +S+ GFAANLTP+QVKKI   PGV+HV +SVN
Sbjct: 61  PETFYIQILASVIDSNEAAKKALVYSFKSSINGFAANLTPNQVKKILAQPGVLHVARSVN 120

Query: 121 YNLQTEGKRDS 132
           YNLQTEGK+++
Sbjct: 121 YNLQTEGKKNN 130

[Close]

BLAST of Lsi03G006480 vs. NCBI nr
Match: gi|307136379|gb|ADN34190.1| (peptidase [Cucumis melo subsp. melo])

HSP 1 Score: 136.7 bits (343), Expect = 2.9e-29
Identity = 75/113 (66.37%), Postives = 91/113 (80.53%), Query Frame = 1


Query: 19  LTISLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEKPEAFNLQILTSVLGSKEA 78
           +++SLN LL+    S +S  S+  + SSLHIVYT++RPSNEKPEAF +QIL SVLGS EA
Sbjct: 19  VSVSLNSLLAWGMISEKSM-SKFAVPSSLHIVYTAKRPSNEKPEAFYIQILASVLGSNEA 78

Query: 79  AKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVNYNLQTEGKRDS 132
           A+KALVYSF NSM GFAANLTP+QVKKIS  PGV+HV +SV YNLQT GK ++
Sbjct: 79  ARKALVYSFKNSMNGFAANLTPNQVKKISAQPGVLHVARSVTYNLQTGGKENN 130

[Close]

BLAST of Lsi03G006480 vs. NCBI nr
Match: gi|449443859|ref|XP_004139693.1| (PREDICTED: subtilisin-like protease SBT3.3 isoform X2 [Cucumis sativus])

HSP 1 Score: 107.5 bits (267), Expect = 1.9e-20
Identity = 66/124 (53.23%), Postives = 91/124 (73.39%), Query Frame = 1


Query: 9   SSFTLYLLLLLTISLNLLLSSTTTSVRSTTSEIGLLSS-----LHIVYTSERPSNEKPEA 68
           ++  + LL L +ISLN  LSST  S  S ++ I L+SS     +HIVYT ERP NE+PEA
Sbjct: 2   ANLNISLLFLFSISLNFHLSSTAMS--SQSASIPLVSSFDSPAVHIVYT-ERPHNEEPEA 61

Query: 69  FNLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVNYNL 128
           ++++ L SVLGS+EAA++ALVYS+ N+ +GF+A LTPDQV +I++ PGV+HVVQS N  L
Sbjct: 62  YHIRTLVSVLGSEEAAREALVYSYKNAASGFSARLTPDQVTEITKQPGVLHVVQSGNNKL 121

[Close]

BLAST of Lsi03G006480 vs. NCBI nr
Match: gi|659123012|ref|XP_008461444.1| (PREDICTED: xylem serine proteinase 1-like isoform X1 [Cucumis melo])

HSP 1 Score: 106.3 bits (264), Expect = 4.2e-20
Identity = 66/124 (53.23%), Postives = 91/124 (73.39%), Query Frame = 1


Query: 9   SSFTLYLLLLLTISLNLLLSSTTTSVRSTTSEIGLLSS-----LHIVYTSERPSNEKPEA 68
           ++  + LL L +ISLNL LSS   S  S +S I L+S+     +HIVYT ERP NE PEA
Sbjct: 2   ANLNVSLLFLFSISLNLHLSSAAMS--SQSSSIPLVSNFDSPAVHIVYT-ERPHNEAPEA 61

Query: 69  FNLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVNYNL 128
           ++++ L SVLGS+EAA++ALVYS+ N+ +GF+A LTPDQVK+I++ PGV+HVVQS +  L
Sbjct: 62  YHIRTLVSVLGSEEAAREALVYSYKNAASGFSARLTPDQVKEITKQPGVLHVVQSGSNKL 121

The following BLAST results are available for this feature:

BLAST of Lsi03G006480 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SBT3B_ARATH	7.2e-12	45.24	Subtilisin-like protease SBT3.11 OS=Arabidopsis thaliana GN=SBT3.11 PE=2 SV=1	[more]
SBT3D_ARATH	4.0e-10	41.46	Subtilisin-like protease SBT3.13 OS=Arabidopsis thaliana GN=SBT3.13 PE=2 SV=1	[more]
SBT36_ARATH	1.5e-09	38.21	Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana GN=SBT3.6 PE=2 SV=1	[more]
SBT33_ARATH	2.0e-09	39.25	Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana GN=SBT3.3 PE=2 SV=1	[more]
SBT34_ARATH	4.4e-09	35.14	Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana GN=SBT3.4 PE=3 SV=1	[more]

BLAST of Lsi03G006480 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K630_CUCSA	2.3e-33	64.89	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G274680 PE=4 SV=1	[more]
E5GCJ1_CUCME	2.0e-29	66.37	Peptidase OS=Cucumis melo subsp. melo PE=4 SV=1	[more]
A0A0A0K6B0_CUCSA	1.3e-20	53.23	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G291690 PE=4 SV=1	[more]
E5GCJ0_CUCME	2.6e-16	53.49	Putative peptidase OS=Cucumis melo subsp. melo PE=4 SV=1	[more]
A5BSE8_VITVI	7.5e-16	55.56	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0117g00130 PE=4 SV=...	[more]

BLAST of Lsi03G006480 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G71950.1	1.1e-15	38.60	Proteinase inhibitor, propeptide	[more]
AT5G11940.1	4.1e-13	45.24	Subtilase family protein	[more]
AT4G21650.1	2.2e-11	41.46	Subtilase family protein	[more]
AT4G10550.3	2.2e-11	41.24	Subtilase family protein	[more]
AT1G32960.1	1.1e-10	39.25	Subtilase family protein	[more]

BLAST of Lsi03G006480 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659124047\|ref\|XP_008461963.1\|	3.2e-36	68.70	PREDICTED: xylem serine proteinase 1-like [Cucumis melo]	[more]
gi\|700189146\|gb\|KGN44379.1\|	3.3e-33	64.89	hypothetical protein Csa_7G274680 [Cucumis sativus]	[more]
gi\|307136379\|gb\|ADN34190.1\|	2.9e-29	66.37	peptidase [Cucumis melo subsp. melo]	[more]
gi\|449443859\|ref\|XP_004139693.1\|	1.9e-20	53.23	PREDICTED: subtilisin-like protease SBT3.3 isoform X2 [Cucumis sativus]	[more]
gi\|659123012\|ref\|XP_008461444.1\|	4.2e-20	53.23	PREDICTED: xylem serine proteinase 1-like isoform X1 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR015500	Peptidase_S8_subtilisin-rel
IPR010259	S8pro/Inhibitor_I9

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0008233	peptidase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G006480.1	Lsi03G006480.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	GENE3D	G3DSA:3.30.70.80		coord: 81..118 score: 1.
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 64..123 score: 2.8
IPR015500	Peptidase S8, subtilisin-related	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 47..125 score: 2.0E-18coord: 7..31 score: 2.0
None	No IPR available	PANTHER	PTHR10795:SF316	SERINE PROTEASE-LIKE PROTEIN-RELATED	coord: 47..125 score: 2.0E-18coord: 7..31 score: 2.0

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi03G006480	Silver-seed gourd	carlsiB412
Lsi03G006480	Silver-seed gourd	carlsiB671
Lsi03G006480	Cucumber (Chinese Long) v3	cuclsiB552
Lsi03G006480	Cucumber (Chinese Long) v3	cuclsiB555
Lsi03G006480	Wax gourd	lsiwgoB307
Lsi03G006480	Wax gourd	lsiwgoB320
Lsi03G006480	Bottle gourd (USVL1VR-Ls)	lsilsiB126
Lsi03G006480	Cucumber (Gy14) v1	cgylsiB364
Lsi03G006480	Cucumber (Gy14) v1	cgylsiB367
Lsi03G006480	Cucurbita maxima (Rimu)	cmalsiB116
Lsi03G006480	Cucurbita maxima (Rimu)	cmalsiB484
Lsi03G006480	Cucurbita maxima (Rimu)	cmalsiB662
Lsi03G006480	Cucurbita moschata (Rifu)	cmolsiB103
Lsi03G006480	Cucurbita moschata (Rifu)	cmolsiB468
Lsi03G006480	Cucurbita moschata (Rifu)	cmolsiB650
Lsi03G006480	Cucurbita pepo (Zucchini)	cpelsiB208
Lsi03G006480	Cucurbita pepo (Zucchini)	cpelsiB543
Lsi03G006480	Wild cucumber (PI 183967)	cpilsiB532
Lsi03G006480	Wild cucumber (PI 183967)	cpilsiB536
Lsi03G006480	Cucumber (Chinese Long) v2	culsiB518
Lsi03G006480	Cucumber (Chinese Long) v2	culsiB522
Lsi03G006480	Melon (DHL92) v3.5.1	lsimeB247
Lsi03G006480	Melon (DHL92) v3.5.1	lsimeB251
Lsi03G006480	Melon (DHL92) v3.5.1	lsimeB268
Lsi03G006480	Watermelon (Charleston Gray)	lsiwcgB223
Lsi03G006480	Watermelon (97103) v1	lsiwmB245
Lsi03G006480	Cucumber (Gy14) v2	cgyblsiB485
Lsi03G006480	Cucumber (Gy14) v2	cgyblsiB490
Lsi03G006480	Melon (DHL92) v3.6.1	lsimedB260
Lsi03G006480	Melon (DHL92) v3.6.1	lsimedB264
Lsi03G006480	Melon (DHL92) v3.6.1	lsimedB281