Lsi03G004720 (gene) Bottle gourd (USVL1VR-Ls)

BLAST of Lsi03G004720 vs. Swiss-Prot
Match: EIF2D_RAT (Eukaryotic translation initiation factor 2D OS=Rattus norvegicus GN=Eif2d PE=1 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 8.4e-55
Identity = 191/633 (30.17%), Postives = 296/633 (46.76%), Query Frame = 1

Query: 1   MFKKAAEAKLHQRLSGADRKKLKRTVKERFPGASDADIDVLLPPKVEFSVAKFQ----NR 60
           MF KA   K +  + G+DR+KL+  V   FP      +  L+P K E +V K      + 
Sbjct: 1   MFAKAFRVKSNTAIKGSDRRKLRADVTVAFPTLGTDQVSELIPGKEELNVVKLYAHKGDA 60

Query: 61  VHIYSLEGGFPMFFDVDGRGTEIFPTVFALWMVPELLPAFVLKGGEVSRFVIGGADLMFP 120
           V +Y+  GG P+ F+++     ++PTV+ LW  P+LLPAF+     + + V GGADLM P
Sbjct: 61  VTVYT-SGGNPILFELE---KNLYPTVYTLWSHPDLLPAFITWPLVLEKLV-GGADLMLP 120

Query: 121 GIQVPAEGLPSFLSGVPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDS 180
           G+ VP  GLP    G   A+ + GN +P+AVGV  MS+ + L +GL+GK + +LH Y+D 
Sbjct: 121 GVVVPPTGLPQVQQGDLCAIALVGNRAPVAVGVAAMSTAQMLASGLKGKGISVLHTYQDH 180

Query: 181 LWGSVEGHYVPNAGFFEDVVYGDPALASSSQTDDSCEGTETADASNYGQNDS---SNKEV 240
           LW S +                 PA+A    T DSCE  E AD   +G   S     +E 
Sbjct: 181 LWRSGDKS-------------SPPAIAPLDPT-DSCE--EKADLGLHGNLRSLSLEGEEE 240

Query: 241 GGSVDETTSDVLDEPGVVLTAQRDSTTDDIVSGVNDLKVVNDISAEEPNVQHTLSAEDVD 300
            G V     +  D+P                         N  +  + +V      E +D
Sbjct: 241 NGQV--PNPEASDDP-------------------------NSRALSQDSVDGKPLQEQMD 300

Query: 301 KLLDKCLLQALHTTVKDKDFPLPGSTLCSGGRLEHGGGVEQVLTATTSVPVRRTGKASFE 360
           +LL++C L AL + VK  D PL  STL        G  +         + ++   K+S++
Sbjct: 301 ELLEQCFLHALKSRVKKADLPLLTSTLL-------GSHMFSCCPEGQQLDIK---KSSYK 360

Query: 361 LQLLFREVHDKLGDDFIVKEDKHKKETVLYSVNRKHPDYTSF-KPEKQQVEKIDQSNNHG 420
               F + H +      VKE     E+++ +V+ +HP  TSF  PE     +  Q  +  
Sbjct: 361 KLSKFLQ-HMQQEQIVQVKELSKGVESIV-AVDWRHPRITSFIVPEPSLASQTVQEGSRE 420

Query: 421 KKESSSNKMLEVAEIYKPSVHVNPIFLAVGADASKLYTAAEATDIVFQYVEKEKLEKPTE 480
           K         ++  +Y    ++  +FL  G         +E   I+  Y ++  L     
Sbjct: 421 KPYLPP----DIKSLYCVPANMTQLFLESGHKKGSTLEGSEVRRIITDYAKRNNLVDADN 480

Query: 481 KSRVVLDAILCDALFKGAIKKGTTYPTEIHKKDIGSTFISRMQPHHIVT-RGSESVVRKG 540
           ++ V LD ILCD + +   K       ++    + +  +  +QP + VT  G E +++KG
Sbjct: 481 RNLVKLDPILCDCILE---KNEQHLVMKLPWDSLLTRCLKNLQPAYQVTFPGQEPIIKKG 540

Query: 541 ALKTIQIMTERRQGNKKVTRLSGLETFLLDAEALASELQKKCACSTTVAELPG-KKGYEV 600
            L  I I    +  NKKVT +  LET+ LD  ++A+ LQ++C  ST V+  PG K   +V
Sbjct: 541 KLCPIDITLVLKTYNKKVTVVRNLETYGLDPCSVAAILQQRCQASTIVSPAPGAKDSLQV 566

Query: 601 VVQGGVIDDLARHLIEQYGIPKKYIEVLDKTRK 624
            VQG  I  L + L+E+Y +P KYI+ L+K  K
Sbjct: 601 QVQGNQIHHLGQLLLEEYRLPGKYIQGLEKAPK 566

BLAST of Lsi03G004720 vs. Swiss-Prot
Match: EIF2D_MOUSE (Eukaryotic translation initiation factor 2D OS=Mus musculus GN=Eif2d PE=1 SV=3)

HSP 1 Score: 212.6 bits (540), Expect = 1.2e-53
Identity = 188/634 (29.65%), Postives = 299/634 (47.16%), Query Frame = 1

Query: 1   MFKKAAEAKLHQRLSGADRKKLKRTVKERFPGASDADIDVLLPPKVEFSVAKFQ----NR 60
           MF KA   K +  + G+DR+KL+  V   FP      I  L+P K E +V K      + 
Sbjct: 1   MFAKAFRVKSNTAIKGSDRRKLRADVTAAFPALGTDQISELIPGKEELNVVKLYVHKGDS 60

Query: 61  VHIYSLEGGFPMFFDVDGRGTEIFPTVFALWMVPELLPAFVLKGGEVSRFVIGGADLMFP 120
           V +Y+  GG P+ F+++     ++PTV+ LW  P++LP F+     + + V GGADLM P
Sbjct: 61  VTVYT-SGGNPILFELE---KNLYPTVYTLWAYPDILPTFITWPLVLEKLV-GGADLMLP 120

Query: 121 GIQVPAEGLPSFLSGVPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDS 180
           G+ VP  GLP    G   A+ + GN +P+A+GV  MS+ + L +GL+GK + +LH Y+D 
Sbjct: 121 GVVVPPTGLPQVQQGDLCAIALVGNRAPVAIGVAAMSTAQMLASGLKGKGVSVLHTYQDH 180

Query: 181 LWGSVEGHYVPNAGFFEDVVYGDPALASSSQTDDSCEGTETADASNYGQNDSSNKEVGGS 240
           LW S +                 PA+A    T DSCE             +  +  + G+
Sbjct: 181 LWRSGDKS-------------SPPAIAPLDPT-DSCE-------------EKVHLGLQGN 240

Query: 241 VDETTSDVLDEPGVVLTAQRDSTTDDIVSGVNDLKVVNDISAEEPNVQHTLSAEDVDKLL 300
           +   T D  +E G V   +    T          +  +  S +   +Q     E +D LL
Sbjct: 241 LKSLTLDGEEENGQVPLREASEDTSS--------RAPSQDSLDGKPLQ-----EQMDDLL 300

Query: 301 DKCLLQALHTTVKDKDFPLPGSTLCSGGRLEHGGGVEQVLTATTSVPVRRTGKASFELQL 360
            +C L AL + VK  D PL  STL        G  +         + ++   K+S++   
Sbjct: 301 LRCFLHALKSRVKKADLPLLTSTLL-------GSHMFSCCPEGQQLDIK---KSSYKKLS 360

Query: 361 LFREVHDKLGDDFIVKEDKHKKETVLYSVNRKHPDYTSFK-PE----KQQVEKIDQSNNH 420
            F + H +      VKE     E+++ +V+ +HP  TSF  PE     Q V+++ +   +
Sbjct: 361 KFLQ-HMQQEQIVQVKELSKGVESIV-AVDWRHPRITSFVIPEPSLTSQTVQEVSREQPY 420

Query: 421 GKKESSSNKMLEVAEIYKPSVHVNPIFLAVGADASKLYTAAEATDIVFQYVEKEKLEKPT 480
                      ++  +Y    ++  +FL  G         +E   I+  Y ++ +L    
Sbjct: 421 LPP--------DIKSLYCVPANMTQLFLESGHKKGSTLEGSEVRKIITDYAKRNRLVDAD 480

Query: 481 EKSRVVLDAILCDALFKGAIKKGTTYPTEIHKKDIGSTFISRMQPHHIVT-RGSESVVRK 540
            ++ V LD ILCD + +   K      T++    + +  +  MQP + VT  G E +++K
Sbjct: 481 NRNLVKLDPILCDCILE---KNEQHLVTKLPWDCLLTRCLKNMQPAYQVTFPGQEPILKK 540

Query: 541 GALKTIQIMTERRQGNKKVTRLSGLETFLLDAEALASELQKKCACSTTVAELPG-KKGYE 600
           G L  I I    +  NKKVT +  LET+ LD  ++A+ LQ++C  ST V+  PG K   +
Sbjct: 541 GKLCPIDITLALKTYNKKVTVVRNLETYGLDPCSVAAILQQRCQASTIVSPAPGAKDSLQ 566

Query: 601 VVVQGGVIDDLARHLIEQYGIPKKYIEVLDKTRK 624
           V VQG  I  L + L+E+Y +P KYI+ L+K  K
Sbjct: 601 VQVQGNQIHHLGQLLLEEYRLPGKYIQGLEKAPK 566

BLAST of Lsi03G004720 vs. Swiss-Prot
Match: EIF2D_BOVIN (Eukaryotic translation initiation factor 2D OS=Bos taurus GN=EIF2D PE=2 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 1.2e-53
Identity = 185/632 (29.27%), Postives = 291/632 (46.04%), Query Frame = 1

Query: 1   MFKKAAEAKLHQRLSGADRKKLKRTVKERFPGASDADIDVLLPPKVEFSVAKFQ----NR 60
           MF KA   K +  + G+DR+KL+  V   FP      +  L+P K E ++ K      + 
Sbjct: 1   MFAKAFRVKSNTAIKGSDRRKLRADVAAVFPTLGTDQVSELVPGKEELNIVKLYAHRGDA 60

Query: 61  VHIYSLEGGFPMFFDVDGRGTEIFPTVFALWMVPELLPAFVLKGGEVSRFVIGGADLMFP 120
           V +Y + GG P+ F+++     ++PTV+ LW  P+LLP F      + + V GGADLM P
Sbjct: 61  VTVY-VSGGNPILFELE---KNLYPTVYTLWSYPDLLPTFTTWPLVLEKLV-GGADLMLP 120

Query: 121 GIQVPAEGLPSFLSGVPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDS 180
           G+ VP  GLP    G   AV + GN +P+AVGV  MS+ E L +GL+G+   +LH Y+D 
Sbjct: 121 GLVVPPAGLPQVQKGDLCAVALVGNRAPVAVGVAAMSTAEMLASGLKGRGFCVLHSYQDH 180

Query: 181 LWGSVEGHYVPNAGFFEDVVYGDPALASSSQTDDSCEGTETADASNYGQNDSSNKEVGGS 240
           LW S +    P+      +    P L+        C   +TA      Q     +     
Sbjct: 181 LWRSGDKSSPPS---IAPLALNPPDLSEGK----GCVKADTALQGAMRQLTLEEEVQQRC 240

Query: 241 VDETTSDVLDEPGVVLTAQRDSTTDDIVSGVNDLKVVNDISAEEPNVQHTLSAEDVDKLL 300
            +++ S+  ++PG                G++           +P    TL  E +D+LL
Sbjct: 241 EEKSPSEATEDPG--------------PGGLH----------VDPMDSKTLQ-EQMDELL 300

Query: 301 DKCLLQALHTTVKDKDFPLPGSTLCSGGRLEHGGGVEQVLTATTSVPVRRTG--KASFEL 360
             C L AL  +V+  D PL  STL        G  +         + ++++   K S  L
Sbjct: 301 QTCFLHALKCSVRKADLPLLTSTLL-------GSHMFSCCPEGRQLDIKKSSYKKLSKFL 360

Query: 361 QLLFREVHDKLGDDFIVKEDKHKKETVLYSVNRKHPDYTSFK-PEKQQVEKIDQSNNHGK 420
           Q + +E         I  ++  K    + +V+ KHP  TSF  PE        Q+   G 
Sbjct: 361 QHMQQE-------QIIQVQELSKGVESIVAVDWKHPRITSFVIPEPSPT---SQTIQEGS 420

Query: 421 KESSSNKMLEVAEIYKPSVHVNPIFLAVGADASKLYTAAEATDIVFQYVEKEKLEKPTEK 480
           +E   +   ++  +Y     +  +F   G     +   +E    V  Y +K  L     K
Sbjct: 421 REQPYHPP-DIKPLYCVPASMTLLFQESGHKKGSVLEGSEVRTFVINYAKKNDLVDADNK 480

Query: 481 SRVVLDAILCDALFKGAIKKGTTYPTEIHKKDIGSTFISRMQPHHIVT-RGSESVVRKGA 540
           + V LD ILCD + +   K      T++    +    + ++QP + VT  G E +V+KG 
Sbjct: 481 NLVKLDPILCDCILE---KDEQHTVTKLPWDSLLGRCLEKLQPAYQVTFPGQEPIVKKGR 540

Query: 541 LKTIQIMTERRQGNKKVTRLSGLETFLLDAEALASELQKKCACSTTVAELPG-KKGYEVV 600
           +  I I   ++  NKKVT +  LE + LD  ++A+ LQ++C  STTV   PG K   +V 
Sbjct: 541 ICPIDITLAQKASNKKVTVVRNLEAYGLDPRSVAATLQQRCQASTTVTSAPGLKDSVQVQ 574

Query: 601 VQGGVIDDLARHLIEQYGIPKKYIEVLDKTRK 624
           +QG  I  L R L+E+Y +P+K+I+ L+K  K
Sbjct: 601 IQGNQIHHLGRLLLEEYRLPRKHIQGLEKAPK 574

BLAST of Lsi03G004720 vs. Swiss-Prot
Match: EIF2D_PONAB (Eukaryotic translation initiation factor 2D OS=Pongo abelii GN=EIF2D PE=2 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 2.1e-53
Identity = 182/632 (28.80%), Postives = 292/632 (46.20%), Query Frame = 1

Query: 1   MFKKAAEAKLHQRLSGADRKKLKRTVKERFPGASDADIDVLLPPKVEFSVAKFQ----NR 60
           MF KA   K +  + G+DR+KL+  V   FP      +  L+P K E ++ K      + 
Sbjct: 1   MFAKAFRVKSNTAIKGSDRRKLRADVTTAFPTLGTDQVSELVPGKEELNIVKLYAHKGDA 60

Query: 61  VHIYSLEGGFPMFFDVDGRGTEIFPTVFALWMVPELLPAFVLKGGEVSRFVIGGADLMFP 120
           V +Y + GG P+ F+++     ++PTV+ LW  P+LLP F      + + V GGADLM P
Sbjct: 61  VTVY-VSGGNPILFELE---KNLYPTVYTLWSYPDLLPTFTTWPLVLEKLV-GGADLMLP 120

Query: 121 GIQVPAEGLPSFLSGVPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDS 180
           G+ +P  GLP    G   A+ + GN +P+A+GV  MS+ E L +GL+G+   +LH Y+D 
Sbjct: 121 GLVMPPAGLPQVQKGDLCAISLVGNRAPVAIGVAAMSTAEMLTSGLKGRGFSVLHTYQDH 180

Query: 181 LWGSVEGHYVPNAGFFEDVVYGDPALASSSQTDDSCEGTETADASNYGQNDSSNKEVGGS 240
           LW S                        SS    +    ++AD S    +   +  + G 
Sbjct: 181 LWRSGN---------------------KSSPPSIAPLALDSADLSEEKGSVQMDSTLQGD 240

Query: 241 VDETTSDVLDEPGVVLTAQRDSTTDDIVSGVNDLKVVNDISAEEPNVQHTLSAEDVDKLL 300
           +   T +  +E G V  A+ D +  +     +  + +N  S +   +Q     E +D+LL
Sbjct: 241 MRHMTLEGEEENGEVHQAREDKSLSEAPEDTS-TRGLNQDSTDSKTLQ-----EQMDELL 300

Query: 301 DKCLLQALHTTVKDKDFPLPGSTLCSGGRLEHGGGVEQVLTATTSVPVRRTG--KASFEL 360
            +C L AL   VK  D PL  ST         G  +         + ++++   K S  L
Sbjct: 301 QQCFLHALKCRVKKADLPLLASTFL-------GSHMFSCCPEGRQLDIKKSSYKKLSKFL 360

Query: 361 QLLFREVHDKLGDDFIVKEDKHKKETVLYSVNRKHPDYTSFK-PEKQQVEKIDQSNNHGK 420
           Q + +E         I  ++  K    + +V+ KHP  TSF  PE        Q+   G 
Sbjct: 361 QQMQQE-------QIIQVKELSKGVESIVAVDWKHPRITSFVIPEPSPT---SQTIQEGS 420

Query: 421 KESSSNKMLEVAEIYKPSVHVNPIFLAVGADASKLYTAAEATDIVFQYVEKEKLEKPTEK 480
           +E   +   ++  +Y     +  +F   G         +E   IV  Y +K  L     K
Sbjct: 421 REQPYHPP-DIKPLYCVPASMTLLFQESGHKKGSFLEGSEVRTIVINYAKKNDLVDADNK 480

Query: 481 SRVVLDAILCDALFKGAIKKGTTYPTEIHKKDIGSTFISRMQPHHIVT-RGSESVVRKGA 540
           + V LD ILCD + +   K       ++    + +  + ++QP + VT  G E +V+KG 
Sbjct: 481 NLVRLDPILCDCILE---KNEQHTVMKLPWDSLLTRCLEKLQPAYQVTLPGQEPIVKKGR 540

Query: 541 LKTIQIMTERRQGNKKVTRLSGLETFLLDAEALASELQKKCACSTTVAELPG-KKGYEVV 600
           +  I I   +R  NKKVT +  LE + LD  ++A+ LQ++C  STTV   PG K   +V 
Sbjct: 541 ICPIDITLAQRASNKKVTVVRNLEAYGLDPYSVAAILQQRCQASTTVNPAPGAKDSLQVQ 579

Query: 601 VQGGVIDDLARHLIEQYGIPKKYIEVLDKTRK 624
           +QG  +  L   L+E+Y +P+K+I+ L+K  K
Sbjct: 601 IQGNQVHHLGWLLLEEYQLPRKHIQGLEKALK 579

BLAST of Lsi03G004720 vs. Swiss-Prot
Match: EIF2D_HUMAN (Eukaryotic translation initiation factor 2D OS=Homo sapiens GN=EIF2D PE=1 SV=3)

HSP 1 Score: 211.1 bits (536), Expect = 3.5e-53
Identity = 182/632 (28.80%), Postives = 292/632 (46.20%), Query Frame = 1

Query: 1   MFKKAAEAKLHQRLSGADRKKLKRTVKERFPGASDADIDVLLPPKVEFSVAKFQ----NR 60
           MF KA   K +  + G+DR+KL+  V   FP      +  L+P K E ++ K      + 
Sbjct: 1   MFAKAFRVKSNTAIKGSDRRKLRADVTTAFPTLGTDQVSELVPGKEELNIVKLYAHKGDA 60

Query: 61  VHIYSLEGGFPMFFDVDGRGTEIFPTVFALWMVPELLPAFVLKGGEVSRFVIGGADLMFP 120
           V +Y + GG P+ F+++     ++PTV+ LW  P+LLP F      + + V GGADLM P
Sbjct: 61  VTVY-VSGGNPILFELE---KNLYPTVYTLWSYPDLLPTFTTWPLVLEKLV-GGADLMLP 120

Query: 121 GIQVPAEGLPSFLSGVPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDS 180
           G+ +P  GLP    G   A+ + GN +P+A+GV  MS+ E L +GL+G+   +LH Y+D 
Sbjct: 121 GLVMPPAGLPQVQKGDLCAISLVGNRAPVAIGVAAMSTAEMLTSGLKGRGFSVLHTYQDH 180

Query: 181 LWGSVEGHYVPNAGFFEDVVYGDPALASSSQTDDSCEGTETADASNYGQNDSSNKEVGGS 240
           LW S                        SS    +    ++AD S    +   +  + G 
Sbjct: 181 LWRSGN---------------------KSSPPSIAPLALDSADLSEEKGSVQMDSTLQGD 240

Query: 241 VDETTSDVLDEPGVVLTAQRDSTTDDIVSGVNDLKVVNDISAEEPNVQHTLSAEDVDKLL 300
           +   T +  +E G V  A+ D +  +     +  + +N  S +   +Q     E +D+LL
Sbjct: 241 MRHMTLEGEEENGEVHQAREDKSLSEAPEDTS-TRGLNQDSTDSKTLQ-----EQMDELL 300

Query: 301 DKCLLQALHTTVKDKDFPLPGSTLCSGGRLEHGGGVEQVLTATTSVPVRRTG--KASFEL 360
            +C L AL   VK  D PL  ST         G  +         + ++++   K S  L
Sbjct: 301 QQCFLHALKCRVKKADLPLLTSTFL-------GSHMFSCCPEGRQLDIKKSSYKKLSKFL 360

Query: 361 QLLFREVHDKLGDDFIVKEDKHKKETVLYSVNRKHPDYTSFK-PEKQQVEKIDQSNNHGK 420
           Q + +E         I  ++  K    + +V+ KHP  TSF  PE        Q+   G 
Sbjct: 361 QQMQQE-------QIIQVKELSKGVESIVAVDWKHPRITSFVIPEPSPT---SQTIQEGS 420

Query: 421 KESSSNKMLEVAEIYKPSVHVNPIFLAVGADASKLYTAAEATDIVFQYVEKEKLEKPTEK 480
           +E   +   ++  +Y     +  +F   G         +E   IV  Y +K  L     K
Sbjct: 421 REQPYHPP-DIKPLYCVPASMTLLFQESGHKKGSFLEGSEVRTIVINYAKKNDLVDADNK 480

Query: 481 SRVVLDAILCDALFKGAIKKGTTYPTEIHKKDIGSTFISRMQPHHIVT-RGSESVVRKGA 540
           + V LD ILCD + +   K       ++    + +  + ++QP + VT  G E +V+KG 
Sbjct: 481 NLVRLDPILCDCILE---KNEQHTVMKLPWDSLLTRCLEKLQPAYQVTLPGQEPIVKKGR 540

Query: 541 LKTIQIMTERRQGNKKVTRLSGLETFLLDAEALASELQKKCACSTTVAELPG-KKGYEVV 600
           +  I I   +R  NKKVT +  LE + LD  ++A+ LQ++C  STTV   PG K   +V 
Sbjct: 541 ICPIDITLAQRASNKKVTVVRNLEAYGLDPYSVAAILQQRCQASTTVNPAPGAKDSLQVQ 579

Query: 601 VQGGVIDDLARHLIEQYGIPKKYIEVLDKTRK 624
           +QG  +  L   L+E+Y +P+K+I+ L+K  K
Sbjct: 601 IQGNQVHHLGWLLLEEYQLPRKHIQGLEKALK 579

BLAST of Lsi03G004720 vs. TrEMBL
Match: A0A0A0LQM2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G401420 PE=4 SV=1)

HSP 1 Score: 1043.9 bits (2698), Expect = 7.9e-302
Identity = 548/624 (87.82%), Postives = 566/624 (90.71%), Query Frame = 1

Query: 1   MFKKAAEAKLHQRLSGADRKKLKRTVKERFPGASDADIDVLLPPKVEFSVAKFQNRVHIY 60
           MFKKA+EAKL QRLSGADRKKLKRTVKERFP ASDADID+LLPPKVEFSVAKFQNRVHIY
Sbjct: 1   MFKKASEAKLQQRLSGADRKKLKRTVKERFPAASDADIDLLLPPKVEFSVAKFQNRVHIY 60

Query: 61  SLEGGFPMFFDVDGRGTEIFPTVFALWMVPELLPAFVLKGGEVSRFVIGGADLMFPGIQV 120
           S+EGGFPMFFDVDGRG EIFPTVFALWMVPELLPAF LKGGEVSRFVIGGADLMFPGI V
Sbjct: 61  SVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIHV 120

Query: 121 PAEGLPSFLSGVPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDSLWGS 180
           PAEGLPSFLSG PWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRD+LWGS
Sbjct: 121 PAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDTLWGS 180

Query: 181 VEGHYVPNAGFFEDVVYGDPALASSSQTDDSCEGTETADASNYGQNDSSNKEVGGSVDET 240
           VEGHYVPNAGF ED VYGDPALASSSQ DDS E TET DA+NYGQNDS NKEVGGSVDET
Sbjct: 181 VEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEDTETGDATNYGQNDSGNKEVGGSVDET 240

Query: 241 TSDVLDEPGVVLTAQRDSTTDDIVSGVNDLKVVNDISAEEPNVQHTLSAEDVDKLLDKCL 300
           T+DVLDEPGVV TAQ DSTTD+IVSGVNDLKVVNDIS EEPNVQHTLSAEDVDKLLDKCL
Sbjct: 241 TTDVLDEPGVVPTAQTDSTTDEIVSGVNDLKVVNDISTEEPNVQHTLSAEDVDKLLDKCL 300

Query: 301 LQALHTTVKDKDFPLPGSTLCSGGRLEHGGGVEQVLTATTSVPVRRTGKASFELQLLFRE 360
           LQALH TVKDKDFPLPGSTL S            VL       +    K+S++    + +
Sbjct: 301 LQALHITVKDKDFPLPGSTLWS----------NHVLPCRPPGMMLDIKKSSYKKLSKWLQ 360

Query: 361 VHDKLGDDFIVKEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNN-HGKKESSSN 420
                G    VKEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNN HG KESSSN
Sbjct: 361 AKSSAG-LITVKEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHHGLKESSSN 420

Query: 421 KMLEVAEIYKPSVHVNPIFLAVGADASKLYTAAEATDIVFQYVEKEKLEKPTEKSRVVLD 480
           KMLEVAEIYKPSVHVNPIF+AVGAD SKLYT+AEATDIVFQYVEKEKLEKPT+KSRVVLD
Sbjct: 421 KMLEVAEIYKPSVHVNPIFVAVGADTSKLYTSAEATDIVFQYVEKEKLEKPTDKSRVVLD 480

Query: 481 AILCDALFKGAIKKGTTYPTEIHKKDIGSTFISRMQPHHIVTRGSESVVRKGALKTIQIM 540
           AILCD+LFKGAIKKGTTYPTEIHK+DIGSTFISRMQPHHIVTRGSESVVRKGALKTIQIM
Sbjct: 481 AILCDSLFKGAIKKGTTYPTEIHKRDIGSTFISRMQPHHIVTRGSESVVRKGALKTIQIM 540

Query: 541 TERRQGNKKVTRLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDD 600
           TERRQGNKKVTRLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDD
Sbjct: 541 TERRQGNKKVTRLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDD 600

Query: 601 LARHLIEQYGIPKKYIEVLDKTRK 624
           LARHLIEQYGIPKKYIEVLDKTRK
Sbjct: 601 LARHLIEQYGIPKKYIEVLDKTRK 613

BLAST of Lsi03G004720 vs. TrEMBL
Match: D7SN00_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0089g00360 PE=4 SV=1)

HSP 1 Score: 820.1 bits (2117), Expect = 1.9e-234
Identity = 435/627 (69.38%), Postives = 501/627 (79.90%), Query Frame = 1

Query: 1   MFKKAAEAKLHQRLSGADRKKLKRTVKERFPGASDADIDVLLPPKVEFSVAKFQNRVHIY 60
           MFKK+ EAK  QRLSGADRKKLKRT+KERF  ASD++ID +LPPK E ++ K QNRVH+Y
Sbjct: 1   MFKKSLEAKSQQRLSGADRKKLKRTIKERFRNASDSEIDSILPPKAEITIVKLQNRVHLY 60

Query: 61  SLEGGFPMFFDVDGRGTEIFPTVFALWMVPELLPAFVLKGGEVSRFVIGGADLMFPGIQV 120
            +EGGFPMFFD+DGRGTEIFPTV+ALW VP+LLPAF+LKGGEVSRFVIGGADLMFPGI +
Sbjct: 61  GVEGGFPMFFDIDGRGTEIFPTVYALWKVPDLLPAFILKGGEVSRFVIGGADLMFPGISI 120

Query: 121 PAEGLPSFLSGVPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDSLWGS 180
           PAEGLPSF  G PWAVKVPGN +PIAVG TTMSST ALKAGLRGKALRI HYYRDSLW  
Sbjct: 121 PAEGLPSFAEGEPWAVKVPGNLAPIAVGTTTMSSTAALKAGLRGKALRITHYYRDSLWEL 180

Query: 181 VEGHYVPNAGFFEDVVYGDPALASSSQTDDSCEGTETADASNYGQNDSSNKEVGGSVDET 240
           VEGHYVPNAGF EDVV+ DP+L+S+ Q  DSCEG    +AS+  QND SNK+ G SVD  
Sbjct: 181 VEGHYVPNAGFLEDVVFEDPSLSSACQASDSCEGVN--EASSDPQNDISNKKEGESVD-- 240

Query: 241 TSDVLDEPGVVLTAQRDSTTD---DIVSGVNDLKVVNDISAEEPNVQHTLSAEDVDKLLD 300
             D+  E     TAQ D + D    I + V+DLKV ++ S  E  V HTLS EDVD LLD
Sbjct: 241 VLDLTSEANPSSTAQLDVSNDPAEQITANVDDLKVTDEESNVE--VHHTLSTEDVDALLD 300

Query: 301 KCLLQALHTTVKDKDFPLPGSTLCSGGRLEHGGGVEQVLTATTSVPVRRTGKASFELQLL 360
           KCLLQALHTTVKDKD P+PGSTL +G           VL    S       K+S+  + L
Sbjct: 301 KCLLQALHTTVKDKDLPMPGSTLWAG----------HVLPCRPSGITLDIKKSSY--KKL 360

Query: 361 FREVHDKLGDDFI-VKEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHGKKES 420
            + +  K     I VKEDKHKKE VL SVNR HP+YTSF+PEK+QVEK DQ+++H   ES
Sbjct: 361 SKWLQAKSSTTLISVKEDKHKKEVVLNSVNRNHPEYTSFRPEKRQVEKSDQTSDHAASES 420

Query: 421 SSNKMLEVAEIYKPSVHVNPIFLAVGADASKLYTAAEATDIVFQYVEKEKLEKPTEKSRV 480
            S+K LEVAEIYKPSVHVNPIF +VG D  KL++A+EATDIVF+Y+EKE L KPT+KS V
Sbjct: 421 RSHKTLEVAEIYKPSVHVNPIFASVGVDTGKLFSASEATDIVFRYIEKENLVKPTDKSMV 480

Query: 481 VLDAILCDALFKGAIKKGTTYPTEIHKKDIGSTFISRMQPHHIVTRGSESVVRKGALKTI 540
           VLDAILCDALFKGAIKKG+TYPT IHKK++G TF++RMQ HH+VTRG ESVVRKGALK I
Sbjct: 481 VLDAILCDALFKGAIKKGSTYPTAIHKKELGHTFVNRMQAHHVVTRGGESVVRKGALKNI 540

Query: 541 QIMTERRQGNKKVTRLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGV 600
           QIMTERRQGNKKVT++SG E+FL+DAEALASELQKK ACSTTVAELPGKKG+EV+VQGGV
Sbjct: 541 QIMTERRQGNKKVTKVSGFESFLMDAEALASELQKKFACSTTVAELPGKKGHEVLVQGGV 600

Query: 601 IDDLARHLIEQYGIPKKYIEVLDKTRK 624
           I+DLA+HL+EQYGIPK+ IEVLDKT+K
Sbjct: 601 IEDLAKHLVEQYGIPKRCIEVLDKTKK 609

BLAST of Lsi03G004720 vs. TrEMBL
Match: A0A067KH41_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_14805 PE=4 SV=1)

HSP 1 Score: 798.5 bits (2061), Expect = 5.8e-228
Identity = 419/627 (66.83%), Postives = 493/627 (78.63%), Query Frame = 1

Query: 1   MFKKAAEAKLHQRLSGADRKKLKRTVKERFPGASDADIDVLLPPKVEFSVAKFQNRVHIY 60
           MFKKA EAK HQRLSGADRKKLKRT++++FP ASDADID+LLPPK E +VAKFQNRVH++
Sbjct: 1   MFKKAVEAKSHQRLSGADRKKLKRTIRDKFPRASDADIDILLPPKAEITVAKFQNRVHVF 60

Query: 61  SLEGGFPMFFDVDGRGTEIFPTVFALWMVPELLPAFVLKGGEVSRFVIGGADLMFPGIQV 120
            +EGGFPMFFDVDGRGTEIFPTVFALW VPELLP+F+LKGGEVSRFVIGGADLMFPGI V
Sbjct: 61  GVEGGFPMFFDVDGRGTEIFPTVFALWKVPELLPSFMLKGGEVSRFVIGGADLMFPGITV 120

Query: 121 PAEGLPSFLSGVPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDSLWGS 180
           PAEGLPSF +G  WAVKVPGN +PIAVG TTM STEA+KAGLRGKALRI HYYRD LW S
Sbjct: 121 PAEGLPSFSAGEVWAVKVPGNPAPIAVGSTTMKSTEAIKAGLRGKALRITHYYRDLLWES 180

Query: 181 VEGHYVPNAGFFEDVVYGDPALASSSQTDDSCEGTETADASNYGQNDSSNKEVGGSVD-- 240
           VEGHYVPNAGF EDVV+ DPA +SS +  D+CE    A   +  QN+  +KE+G S D  
Sbjct: 181 VEGHYVPNAGFLEDVVFEDPAFSSSVKVPDACE----AGGDDDQQNEDQSKEIGESADLN 240

Query: 241 ETTSDVLDEPGVVLTAQRDSTTDDIVSGVNDLKVVNDISAEEPNV--QHTLSAEDVDKLL 300
           E TS+      V L ++ D   + + + V DLK+ ++I+ +E N   QH LS  DVD  L
Sbjct: 241 EVTSEPHPSSNVKLDSKND-IVEQVSADVGDLKIKDNIATDESNAEGQHMLSTTDVDAYL 300

Query: 301 DKCLLQALHTTVKDKDFPLPGSTLCSGGRLEHGGGVEQVLTATTSVPVRRTGKASFELQL 360
           DKCLLQALH TVKDKD P+PGSTL S            VL    S  +    K+S +   
Sbjct: 301 DKCLLQALHMTVKDKDLPMPGSTLWS----------NHVLPCRPSGVMLDIKKSSHKKLS 360

Query: 361 LFREVHDKLGDDFIVKEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHGKKES 420
            + +     G    VKEDK+KKETVL+S+NR HPDY SFKPEK+Q +KIDQ+ +     S
Sbjct: 361 KWLQAKSSAG-LISVKEDKYKKETVLFSINRAHPDYLSFKPEKRQEQKIDQTADQATSAS 420

Query: 421 SSNKMLEVAEIYKPSVHVNPIFLAVGADASKLYTAAEATDIVFQYVEKEKLEKPTEKSRV 480
              K LEV EIYKPSVHVNPIF +VGAD  KLY+A+EA+DIVF+Y+EKE L KPT K+ V
Sbjct: 421 RPQKTLEVTEIYKPSVHVNPIFASVGADTGKLYSASEASDIVFKYIEKENLVKPTNKAFV 480

Query: 481 VLDAILCDALFKGAIKKGTTYPTEIHKKDIGSTFISRMQPHHIVTRGSESVVRKGALKTI 540
           VLD  LCDALFKGAIKKG+TYPTEIHKKD+G TF+SRMQ H++VTRGSESVVRKG+LK I
Sbjct: 481 VLDPTLCDALFKGAIKKGSTYPTEIHKKDLGLTFVSRMQAHYVVTRGSESVVRKGSLKAI 540

Query: 541 QIMTERRQGNKKVTRLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGV 600
           QI+TERRQGNKKVT++SG+E+FL+DAEALASELQKK ACST+V ELPGKKG+EV++QGGV
Sbjct: 541 QIVTERRQGNKKVTKVSGIESFLMDAEALASELQKKFACSTSVQELPGKKGFEVLIQGGV 600

Query: 601 IDDLARHLIEQYGIPKKYIEVLDKTRK 624
           IDD+ARHL+EQYG+PK+YIEVLDKT+K
Sbjct: 601 IDDVARHLVEQYGVPKRYIEVLDKTKK 611

BLAST of Lsi03G004720 vs. TrEMBL
Match: M5VKY6_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003013mg PE=4 SV=1)

HSP 1 Score: 798.5 bits (2061), Expect = 5.8e-228
Identity = 429/628 (68.31%), Postives = 496/628 (78.98%), Query Frame = 1

Query: 1   MFKKAAEAKLHQRLSGADRKKLKRTVKERFPGASDADIDVLLPPKVEFSVAKFQNRVHIY 60
           MFKKA EA+ HQRLSGADRKKLKR++K +FPGAS++DID LLPPKVE +VAKFQNRVH+Y
Sbjct: 1   MFKKAVEARSHQRLSGADRKKLKRSLKNKFPGASESDIDSLLPPKVEIAVAKFQNRVHVY 60

Query: 61  SLEGGFPMFFDVDGRGTEIFPTVFALWMVPELLPAFVLKGGEVSRFVIGGADLMFPGIQV 120
            +EGGFP+FFD+DGRGT+IFPTV+ALW VP+LLP+FVLKGGEVSR++IGGADLMFPGI +
Sbjct: 61  GVEGGFPIFFDIDGRGTDIFPTVYALWKVPDLLPSFVLKGGEVSRYIIGGADLMFPGISI 120

Query: 121 PAEGLPSFLSGVPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDSLWGS 180
           PAEGLPSF++G  WAVKVPGN +PIAVG TTMSSTEA KAGLRGKAL+I+HYY D LW S
Sbjct: 121 PAEGLPSFIAGEAWAVKVPGNPAPIAVGTTTMSSTEAAKAGLRGKALKIMHYYHDLLWES 180

Query: 181 VEGHYVPNAGFFEDVVYGDPALASSSQTDDSCEGTETADASNYGQNDSSNKEVGGSVDET 240
           VE  YVPNAGFFEDVV+ DP L SS Q  DS  G     +SN  QN  S+KE G SVD  
Sbjct: 181 VECPYVPNAGFFEDVVFEDPTL-SSLQVSDSYSGANY--SSNDQQNGISDKENGKSVD-- 240

Query: 241 TSDVLDEPGVVLTAQRDS---TTDDIVSGVNDLKVVNDISAEEP--NVQHTLSAEDVDKL 300
            +DVL EP     AQ +S     ++I + V+DLKV +D SA++     QH LS EDVD L
Sbjct: 241 VADVLSEPSSASVAQTNSGNEIAEEIAAHVDDLKVTDDGSADQSIGEDQHPLSTEDVDTL 300

Query: 301 LDKCLLQALHTTVKDKDFPLPGSTLCSGGRLEHGGGVEQVLTATTSVPVRRTGKASFELQ 360
           L+KCLLQALHTTVKDKD P+PGSTL S            VL    S       K+S++  
Sbjct: 301 LEKCLLQALHTTVKDKDLPMPGSTLWS----------NHVLPCRPSGITLDIKKSSYKKL 360

Query: 361 LLFREVHDKLGDDFIVKEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHGKKE 420
             + +     G    VKEDK+KKE++L SVNRKHPDY+SFKPEK+QVEK  Q+      E
Sbjct: 361 SKWLQAKSSTG-LITVKEDKYKKESILLSVNRKHPDYSSFKPEKRQVEKTVQTGVPTVSE 420

Query: 421 SSSNKMLEVAEIYKPSVHVNPIFLAVGADASKLYTAAEATDIVFQYVEKEKLEKPTEKSR 480
           S S KMLEV E+YKPSVHVNPI  +VGAD  KLY+A+EATDIVF YVEKE L K T+KS 
Sbjct: 421 SRSLKMLEVVEVYKPSVHVNPILASVGADTGKLYSASEATDIVFTYVEKENLVKQTDKSI 480

Query: 481 VVLDAILCDALFKGAIKKGTTYPTEIHKKDIGSTFISRMQPHHIVTRGSESVVRKGALKT 540
           VVLD ILCDALFKGAIKKGT YPTEIHK+D+G+ F++RMQ HH+VTRGSESVVRKG LKT
Sbjct: 481 VVLDPILCDALFKGAIKKGTMYPTEIHKRDLGAAFVNRMQAHHVVTRGSESVVRKGGLKT 540

Query: 541 IQIMTERRQGNKKVTRLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGG 600
           IQIMTERRQGNKKVT+LSGLETFL+D EALASELQKK ACSTTV ELPGKKG+EVVVQGG
Sbjct: 541 IQIMTERRQGNKKVTKLSGLETFLVDPEALASELQKKFACSTTVVELPGKKGHEVVVQGG 600

Query: 601 VIDDLARHLIEQYGIPKKYIEVLDKTRK 624
           VIDDLA+H+IEQYGIPK++IEVLDKTR+
Sbjct: 601 VIDDLAKHMIEQYGIPKRFIEVLDKTRR 612

BLAST of Lsi03G004720 vs. TrEMBL
Match: A0A061FKJ7_THECC (Eukaryotic translation initiation factor SUI1 family protein isoform 1 OS=Theobroma cacao GN=TCM_036324 PE=4 SV=1)

HSP 1 Score: 797.7 bits (2059), Expect = 9.9e-228
Identity = 422/626 (67.41%), Postives = 497/626 (79.39%), Query Frame = 1

Query: 1   MFKKAAEAKLHQRLSGADRKKLKRTVKERFPGASDADIDVLLPPKVEFSVAKFQNRVHIY 60
           MFKKA EAK HQRLSGADRKKLKRT+++RFP ASDADID LLPPK E +VAKFQNR H+Y
Sbjct: 1   MFKKAVEAKAHQRLSGADRKKLKRTLRDRFPTASDADIDALLPPKTEITVAKFQNRAHVY 60

Query: 61  SLEGGFPMFFDVDGRGTEIFPTVFALWMVPELLPAFVLKGGEVSRFVIGGADLMFPGIQV 120
            +EGGFP+FFDVDGRGTEI+PTVFALW VPEL+P+F+LKGGEVSRFVIGGADLMFPGI V
Sbjct: 61  GVEGGFPVFFDVDGRGTEIYPTVFALWKVPELMPSFMLKGGEVSRFVIGGADLMFPGISV 120

Query: 121 PAEGLPSFLSGVPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDSLWGS 180
            AEGLPSF +G PWAVKVPGN +PIAVG TTMSSTEALKAGLRGKALRI+HYYRD LW S
Sbjct: 121 AAEGLPSFSAGEPWAVKVPGNPAPIAVGSTTMSSTEALKAGLRGKALRIMHYYRDLLWES 180

Query: 181 VEGHYVPNAGFFEDVVYGDPALASSSQTDDSCEGTETADASNYGQNDSSNKEVGGSVDET 240
           VEGHYVPNAGF ED V+ DP+  SS+Q   S EG   AD S+  Q+  +N+++G SV+  
Sbjct: 181 VEGHYVPNAGFLEDAVFEDPSFLSSNQPSVSSEGA--ADGSDAQQSGINNEDIGESVN-- 240

Query: 241 TSDVLDEPGVVLTAQR--DSTTDDIVSGVNDLKVVNDISAEEPNVQH-TLSAEDVDKLLD 300
            +DV+ E  +  +AQ   ++  ++I   V+DLK+  +++A E N +H +LS E VD  LD
Sbjct: 241 VNDVVSER-LAASAQNYSENAAEEITPDVSDLKLSENVAATESNTEHNSLSTEGVDSHLD 300

Query: 301 KCLLQALHTTVKDKDFPLPGSTLCSGGRLEHGGGVEQVLTATTSVPVRRTGKASFELQLL 360
           +CLLQALHTTVKDKD P+PGSTL S            VL    S       K+S +    
Sbjct: 301 RCLLQALHTTVKDKDLPMPGSTLWSN----------HVLACRPSGITLDIKKSSHKKLSK 360

Query: 361 FREVHDKLGDDFIVKEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHGKKESS 420
           + +     G   +VKEDK+KKE +L SVNR HPDY SFKPEK+ VEK+DQ+ +    E  
Sbjct: 361 WLQAKSSTGL-IVVKEDKYKKEAMLISVNRAHPDYLSFKPEKRPVEKVDQAGDSAASECL 420

Query: 421 SNKMLEVAEIYKPSVHVNPIFLAVGADASKLYTAAEATDIVFQYVEKEKLEKPTEKSRVV 480
           S K+LEV E+YK SVHV PIF +VGAD  KLY+A+EA+DIVF+Y+EKE L K T K+ +V
Sbjct: 421 SQKVLEVVEVYKSSVHVKPIFASVGADTGKLYSASEASDIVFKYIEKENLVKQTNKAIMV 480

Query: 481 LDAILCDALFKGAIKKGTTYPTEIHKKDIGSTFISRMQPHHIVTRGSESVVRKGALKTIQ 540
           LDA LCDALFKGAIKKG+TYPTEIHKKD+GSTFISRMQ HHIVTRGSES VRKGALKT+Q
Sbjct: 481 LDATLCDALFKGAIKKGSTYPTEIHKKDLGSTFISRMQAHHIVTRGSESAVRKGALKTVQ 540

Query: 541 IMTERRQGNKKVTRLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVI 600
           I+TERRQGNKKVT++SGLETFL+DAEALASELQKK ACSTTVAELPGKKG+EV++QGGVI
Sbjct: 541 IVTERRQGNKKVTKVSGLETFLVDAEALASELQKKFACSTTVAELPGKKGHEVLIQGGVI 600

Query: 601 DDLARHLIEQYGIPKKYIEVLDKTRK 624
           DD+ARHL+EQYGIPK+YIE LDKTRK
Sbjct: 601 DDVARHLVEQYGIPKRYIEFLDKTRK 610

BLAST of Lsi03G004720 vs. TAIR10
Match: AT1G71350.1 (AT1G71350.1 eukaryotic translation initiation factor SUI1 family protein)

HSP 1 Score: 698.0 bits (1800), Expect = 5.4e-201
Identity = 372/627 (59.33%), Postives = 456/627 (72.73%), Query Frame = 1

Query: 1   MFKKAAEAKLHQRLSGADRKKLKRTVKERFPGASDADIDVLLPPKVEFSVAKFQNRVHIY 60
           MFKKA EAK HQRLSGADRKKL+RTV+ R P  +D  +D +LPPKVE +++KFQNRV +Y
Sbjct: 1   MFKKAVEAKSHQRLSGADRKKLRRTVRNRLPLLTDELLDAILPPKVEITLSKFQNRVIVY 60

Query: 61  SLEGGFPMFFDVDGRGTEIFPTVFALWMVPELLPAFVLKGGEVSRFVIGGADLMFPGIQV 120
           S+EGG PMFFD+DGRGTEIFPTVFALW  PE+LP+F+LKGGEVSR++IGGADLMFPGI +
Sbjct: 61  SIEGGCPMFFDIDGRGTEIFPTVFALWEAPEMLPSFMLKGGEVSRYIIGGADLMFPGILI 120

Query: 121 PAEGLPSFLSGVPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDSLWGS 180
           P +G PSF +G  WAVKVPGN +PIAVG TTMSS EA KAGLRGKALRI HYYRD LW S
Sbjct: 121 PPQGYPSFSAGEIWAVKVPGNLAPIAVGCTTMSSEEARKAGLRGKALRITHYYRDFLWES 180

Query: 181 VEGHYVPNAGFFEDVVYGDPALASSSQTDDSCEGTETADASNYGQNDSSNKEVGGSVDET 240
            EGHYVPNAGF EDVV  DP+  +    +D  +      AS   + D S  E   +   +
Sbjct: 181 AEGHYVPNAGFMEDVVMEDPSYLAPGSGEDILD----TSASTGNEEDESALETAST---S 240

Query: 241 TSDVLDEPGVVLTAQRDSTTDDIVSGVNDLKVVNDISAEEPNV--QHTLSAEDVDKLLDK 300
            +D  ++  V            +V  +N+L +V+D+ A E N   Q TLS E+VD LLD+
Sbjct: 241 AADAKNDAEV-----------HVVGSMNELYLVDDVCATETNTDKQITLSPEEVDTLLDQ 300

Query: 301 CLLQALHTTVKDKDFPLPGSTLCSGGRLEHGGGVEQVLTATTSVPVRRTGKASFELQLLF 360
           CLLQALHTT+K+KD P+PGSTL +            VL    S       K+S   + L 
Sbjct: 301 CLLQALHTTLKEKDLPIPGSTLWA----------NHVLPCRPSGLTLDIKKSSH--KKLS 360

Query: 361 REVHDKLGDDFI-VKEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHGKKESS 420
           + +  K     I VKEDKHKKE VL SVNR HPDY SFKPEK++ E  +        ++ 
Sbjct: 361 KWLQSKASGGMISVKEDKHKKEIVLISVNRSHPDYKSFKPEKKKAEVSESPGERSTAQAQ 420

Query: 421 SNKMLEVAEIYKPSVHVNPIFLAVGADASKLYTAAEATDIVFQYVEKEKLEKPTEKSRVV 480
           S K LE+ E+YK S+H + IF +VG D   LYTA+EATD+VF+Y+EKE L KPT KS VV
Sbjct: 421 SEKGLEIIEVYKSSIHNSAIFASVGEDKGNLYTASEATDVVFKYIEKENLVKPTNKSMVV 480

Query: 481 LDAILCDALFKGAIKKGTTYPTEIHKKDIGSTFISRMQPHHIVTR-GSESVVRKGALKTI 540
           LD ILCDALFKGAIKKG+ YP+EIHKKD+GSTF+ RMQP+H+V R G E VVRKGA+K +
Sbjct: 481 LDPILCDALFKGAIKKGSAYPSEIHKKDVGSTFVGRMQPNHVVMRGGGEPVVRKGAVKPV 540

Query: 541 QIMTERRQGNKKVTRLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGV 600
           QIMTERRQGNKKVT+++G+ETFL+D ++  SELQKK ACST+V ELPGKKGYEV++QGGV
Sbjct: 541 QIMTERRQGNKKVTKVTGMETFLIDPDSFGSELQKKFACSTSVGELPGKKGYEVLIQGGV 597

Query: 601 IDDLARHLIEQYGIPKKYIEVLDKTRK 624
           ID+LAR+++E YG+PK+YIEVLDKTRK
Sbjct: 601 IDNLARYMVEHYGVPKRYIEVLDKTRK 597

BLAST of Lsi03G004720 vs. TAIR10
Match: AT1G09150.1 (AT1G09150.1 pseudouridine synthase and archaeosine transglycosylase (PUA) domain-containing protein)

HSP 1 Score: 67.8 bits (164), Expect = 2.7e-11
Identity = 49/181 (27.07%), Postives = 90/181 (49.72%), Query Frame = 1

Query: 1   MFKKAA--EAKLHQRLSGADRKKLKRTVKERFPGASDADIDVLLPPKVEFSVAKFQNRVH 60
           MFKK    E     ++  + ++++++++++ +PG      D LLP K+   V K  N + 
Sbjct: 1   MFKKFCLEEISSQNQVKASVQRRIRQSIQDEYPGLESVMED-LLPKKIPLIVVKCPNHLT 60

Query: 61  IYSLEGGFPMFFDVDGRGTEIFPTVFALWMVPELLPAFVLKGGEVSRFVIGGADLMFPGI 120
           +  +    P+FF +  R     PT+  L   P ++  F +  G + +FV+ GA++M PG+
Sbjct: 61  LVVVNN-VPLFFCI--RDGPYMPTLRLLHQYPNIMKRFQVDRGAI-KFVLSGANIMCPGL 120

Query: 121 QVPAEGLPSFLSGV-PWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDSL 179
             P   L   +    P A+   G    +A+G T MS+ + +K+  +G  +  +HY  D L
Sbjct: 121 TSPGGVLDQEVEAERPVAIYAEGKQHALAIGFTKMSAKD-IKSINKGIGVDNMHYLNDGL 175

BLAST of Lsi03G004720 vs. NCBI nr
Match: gi|659081170|ref|XP_008441190.1| (PREDICTED: eukaryotic translation initiation factor 2D isoform X1 [Cucumis melo])

HSP 1 Score: 1052.4 bits (2720), Expect = 3.2e-304
Identity = 549/623 (88.12%), Postives = 567/623 (91.01%), Query Frame = 1

Query: 1   MFKKAAEAKLHQRLSGADRKKLKRTVKERFPGASDADIDVLLPPKVEFSVAKFQNRVHIY 60
           MFKKAAEAKL QRLSGADRKKLKRTVKERFPG SDADID+LLPPKVEFSVAKFQNRVHIY
Sbjct: 1   MFKKAAEAKLQQRLSGADRKKLKRTVKERFPGPSDADIDLLLPPKVEFSVAKFQNRVHIY 60

Query: 61  SLEGGFPMFFDVDGRGTEIFPTVFALWMVPELLPAFVLKGGEVSRFVIGGADLMFPGIQV 120
           S+EGGFPMFFDVDGRG EIFPTVFALWMVPELLPAF LKGGEVSRFVIGGADLMFPGI +
Sbjct: 61  SVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIHI 120

Query: 121 PAEGLPSFLSGVPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDSLWGS 180
           PAEGLPSFLSG PWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDSLWGS
Sbjct: 121 PAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDSLWGS 180

Query: 181 VEGHYVPNAGFFEDVVYGDPALASSSQTDDSCEGTETADASNYGQNDSSNKEVGGSVDET 240
           VEGHYVPNAGF ED VYGDPALASSSQ DDS EGTET DASNYGQNDS +KEVGGSVDET
Sbjct: 181 VEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEGTETDDASNYGQNDSGDKEVGGSVDET 240

Query: 241 TSDVLDEPGVVLTAQRDSTTDDIVSGVNDLKVVNDISAEEPNVQHTLSAEDVDKLLDKCL 300
           T+DVLDEPGVVLTAQ DST D+IVSGVNDLKVVNDIS EEP VQHTLSAEDVDKLLDKCL
Sbjct: 241 TTDVLDEPGVVLTAQTDSTIDEIVSGVNDLKVVNDISTEEPKVQHTLSAEDVDKLLDKCL 300

Query: 301 LQALHTTVKDKDFPLPGSTLCSGGRLEHGGGVEQVLTATTSVPVRRTGKASFELQLLFRE 360
           LQALHTTVKDKDFPLPGSTL S            VL       +    K+S++    + +
Sbjct: 301 LQALHTTVKDKDFPLPGSTLWS----------NHVLPCRPPGMMLDIKKSSYKKLSKWLQ 360

Query: 361 VHDKLGDDFIVKEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHGKKESSSNK 420
                G    VKEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHG KESSSNK
Sbjct: 361 AKSSAG-LITVKEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHGPKESSSNK 420

Query: 421 MLEVAEIYKPSVHVNPIFLAVGADASKLYTAAEATDIVFQYVEKEKLEKPTEKSRVVLDA 480
           MLEVAEIYKPSVHVNPIF+AVGAD SKLYT+AEATDIVF+YVEKEKLEKPT+KSRVVLDA
Sbjct: 421 MLEVAEIYKPSVHVNPIFVAVGADTSKLYTSAEATDIVFEYVEKEKLEKPTDKSRVVLDA 480

Query: 481 ILCDALFKGAIKKGTTYPTEIHKKDIGSTFISRMQPHHIVTRGSESVVRKGALKTIQIMT 540
           ILCDALFKGAIKKGTTYPTEIHK+DIGSTFISRMQPHH+VTRGSESVVRKGALKTIQIMT
Sbjct: 481 ILCDALFKGAIKKGTTYPTEIHKRDIGSTFISRMQPHHVVTRGSESVVRKGALKTIQIMT 540

Query: 541 ERRQGNKKVTRLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDL 600
           ERRQGNKKVTRLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDL
Sbjct: 541 ERRQGNKKVTRLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDL 600

Query: 601 ARHLIEQYGIPKKYIEVLDKTRK 624
           ARHLIEQYGIPKKYIEVLDKTRK
Sbjct: 601 ARHLIEQYGIPKKYIEVLDKTRK 612

BLAST of Lsi03G004720 vs. NCBI nr
Match: gi|778672898|ref|XP_011649889.1| (PREDICTED: eukaryotic translation initiation factor 2D [Cucumis sativus])

HSP 1 Score: 1043.9 bits (2698), Expect = 1.1e-301
Identity = 548/624 (87.82%), Postives = 566/624 (90.71%), Query Frame = 1

Query: 1   MFKKAAEAKLHQRLSGADRKKLKRTVKERFPGASDADIDVLLPPKVEFSVAKFQNRVHIY 60
           MFKKA+EAKL QRLSGADRKKLKRTVKERFP ASDADID+LLPPKVEFSVAKFQNRVHIY
Sbjct: 1   MFKKASEAKLQQRLSGADRKKLKRTVKERFPAASDADIDLLLPPKVEFSVAKFQNRVHIY 60

Query: 61  SLEGGFPMFFDVDGRGTEIFPTVFALWMVPELLPAFVLKGGEVSRFVIGGADLMFPGIQV 120
           S+EGGFPMFFDVDGRG EIFPTVFALWMVPELLPAF LKGGEVSRFVIGGADLMFPGI V
Sbjct: 61  SVEGGFPMFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIHV 120

Query: 121 PAEGLPSFLSGVPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDSLWGS 180
           PAEGLPSFLSG PWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRD+LWGS
Sbjct: 121 PAEGLPSFLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDTLWGS 180

Query: 181 VEGHYVPNAGFFEDVVYGDPALASSSQTDDSCEGTETADASNYGQNDSSNKEVGGSVDET 240
           VEGHYVPNAGF ED VYGDPALASSSQ DDS E TET DA+NYGQNDS NKEVGGSVDET
Sbjct: 181 VEGHYVPNAGFLEDAVYGDPALASSSQIDDSYEDTETGDATNYGQNDSGNKEVGGSVDET 240

Query: 241 TSDVLDEPGVVLTAQRDSTTDDIVSGVNDLKVVNDISAEEPNVQHTLSAEDVDKLLDKCL 300
           T+DVLDEPGVV TAQ DSTTD+IVSGVNDLKVVNDIS EEPNVQHTLSAEDVDKLLDKCL
Sbjct: 241 TTDVLDEPGVVPTAQTDSTTDEIVSGVNDLKVVNDISTEEPNVQHTLSAEDVDKLLDKCL 300

Query: 301 LQALHTTVKDKDFPLPGSTLCSGGRLEHGGGVEQVLTATTSVPVRRTGKASFELQLLFRE 360
           LQALH TVKDKDFPLPGSTL S            VL       +    K+S++    + +
Sbjct: 301 LQALHITVKDKDFPLPGSTLWS----------NHVLPCRPPGMMLDIKKSSYKKLSKWLQ 360

Query: 361 VHDKLGDDFIVKEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNN-HGKKESSSN 420
                G    VKEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNN HG KESSSN
Sbjct: 361 AKSSAG-LITVKEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHHGLKESSSN 420

Query: 421 KMLEVAEIYKPSVHVNPIFLAVGADASKLYTAAEATDIVFQYVEKEKLEKPTEKSRVVLD 480
           KMLEVAEIYKPSVHVNPIF+AVGAD SKLYT+AEATDIVFQYVEKEKLEKPT+KSRVVLD
Sbjct: 421 KMLEVAEIYKPSVHVNPIFVAVGADTSKLYTSAEATDIVFQYVEKEKLEKPTDKSRVVLD 480

Query: 481 AILCDALFKGAIKKGTTYPTEIHKKDIGSTFISRMQPHHIVTRGSESVVRKGALKTIQIM 540
           AILCD+LFKGAIKKGTTYPTEIHK+DIGSTFISRMQPHHIVTRGSESVVRKGALKTIQIM
Sbjct: 481 AILCDSLFKGAIKKGTTYPTEIHKRDIGSTFISRMQPHHIVTRGSESVVRKGALKTIQIM 540

Query: 541 TERRQGNKKVTRLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDD 600
           TERRQGNKKVTRLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDD
Sbjct: 541 TERRQGNKKVTRLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDD 600

Query: 601 LARHLIEQYGIPKKYIEVLDKTRK 624
           LARHLIEQYGIPKKYIEVLDKTRK
Sbjct: 601 LARHLIEQYGIPKKYIEVLDKTRK 613

BLAST of Lsi03G004720 vs. NCBI nr
Match: gi|659081172|ref|XP_008441191.1| (PREDICTED: eukaryotic translation initiation factor 2D isoform X2 [Cucumis melo])

HSP 1 Score: 929.5 bits (2401), Expect = 3.1e-267
Identity = 486/556 (87.41%), Postives = 502/556 (90.29%), Query Frame = 1

Query: 68  MFFDVDGRGTEIFPTVFALWMVPELLPAFVLKGGEVSRFVIGGADLMFPGIQVPAEGLPS 127
           MFFDVDGRG EIFPTVFALWMVPELLPAF LKGGEVSRFVIGGADLMFPGI +PAEGLPS
Sbjct: 1   MFFDVDGRGNEIFPTVFALWMVPELLPAFFLKGGEVSRFVIGGADLMFPGIHIPAEGLPS 60

Query: 128 FLSGVPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDSLWGSVEGHYVP 187
           FLSG PWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDSLWGSVEGHYVP
Sbjct: 61  FLSGEPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDSLWGSVEGHYVP 120

Query: 188 NAGFFEDVVYGDPALASSSQTDDSCEGTETADASNYGQNDSSNKEVGGSVDETTSDVLDE 247
           NAGF ED VYGDPALASSSQ DDS EGTET DASNYGQNDS +KEVGGSVDETT+DVLDE
Sbjct: 121 NAGFLEDAVYGDPALASSSQIDDSYEGTETDDASNYGQNDSGDKEVGGSVDETTTDVLDE 180

Query: 248 PGVVLTAQRDSTTDDIVSGVNDLKVVNDISAEEPNVQHTLSAEDVDKLLDKCLLQALHTT 307
           PGVVLTAQ DST D+IVSGVNDLKVVNDIS EEP VQHTLSAEDVDKLLDKCLLQALHTT
Sbjct: 181 PGVVLTAQTDSTIDEIVSGVNDLKVVNDISTEEPKVQHTLSAEDVDKLLDKCLLQALHTT 240

Query: 308 VKDKDFPLPGSTLCSGGRLEHGGGVEQVLTATTSVPVRRTGKASFELQLLFREVHDKLGD 367
           VKDKDFPLPGSTL S            VL       +    K+S++    + +     G 
Sbjct: 241 VKDKDFPLPGSTLWSN----------HVLPCRPPGMMLDIKKSSYKKLSKWLQAKSSAGL 300

Query: 368 DFIVKEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHGKKESSSNKMLEVAEI 427
              VKEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHG KESSSNKMLEVAEI
Sbjct: 301 -ITVKEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHGPKESSSNKMLEVAEI 360

Query: 428 YKPSVHVNPIFLAVGADASKLYTAAEATDIVFQYVEKEKLEKPTEKSRVVLDAILCDALF 487
           YKPSVHVNPIF+AVGAD SKLYT+AEATDIVF+YVEKEKLEKPT+KSRVVLDAILCDALF
Sbjct: 361 YKPSVHVNPIFVAVGADTSKLYTSAEATDIVFEYVEKEKLEKPTDKSRVVLDAILCDALF 420

Query: 488 KGAIKKGTTYPTEIHKKDIGSTFISRMQPHHIVTRGSESVVRKGALKTIQIMTERRQGNK 547
           KGAIKKGTTYPTEIHK+DIGSTFISRMQPHH+VTRGSESVVRKGALKTIQIMTERRQGNK
Sbjct: 421 KGAIKKGTTYPTEIHKRDIGSTFISRMQPHHVVTRGSESVVRKGALKTIQIMTERRQGNK 480

Query: 548 KVTRLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDLARHLIEQ 607
           KVTRLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDLARHLIEQ
Sbjct: 481 KVTRLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGVIDDLARHLIEQ 540

Query: 608 YGIPKKYIEVLDKTRK 624
           YGIPKKYIEVLDKTRK
Sbjct: 541 YGIPKKYIEVLDKTRK 545

BLAST of Lsi03G004720 vs. NCBI nr
Match: gi|225460008|ref|XP_002271249.1| (PREDICTED: eukaryotic translation initiation factor 2D [Vitis vinifera])

HSP 1 Score: 820.1 bits (2117), Expect = 2.7e-234
Identity = 435/627 (69.38%), Postives = 501/627 (79.90%), Query Frame = 1

Query: 1   MFKKAAEAKLHQRLSGADRKKLKRTVKERFPGASDADIDVLLPPKVEFSVAKFQNRVHIY 60
           MFKK+ EAK  QRLSGADRKKLKRT+KERF  ASD++ID +LPPK E ++ K QNRVH+Y
Sbjct: 1   MFKKSLEAKSQQRLSGADRKKLKRTIKERFRNASDSEIDSILPPKAEITIVKLQNRVHLY 60

Query: 61  SLEGGFPMFFDVDGRGTEIFPTVFALWMVPELLPAFVLKGGEVSRFVIGGADLMFPGIQV 120
            +EGGFPMFFD+DGRGTEIFPTV+ALW VP+LLPAF+LKGGEVSRFVIGGADLMFPGI +
Sbjct: 61  GVEGGFPMFFDIDGRGTEIFPTVYALWKVPDLLPAFILKGGEVSRFVIGGADLMFPGISI 120

Query: 121 PAEGLPSFLSGVPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDSLWGS 180
           PAEGLPSF  G PWAVKVPGN +PIAVG TTMSST ALKAGLRGKALRI HYYRDSLW  
Sbjct: 121 PAEGLPSFAEGEPWAVKVPGNLAPIAVGTTTMSSTAALKAGLRGKALRITHYYRDSLWEL 180

Query: 181 VEGHYVPNAGFFEDVVYGDPALASSSQTDDSCEGTETADASNYGQNDSSNKEVGGSVDET 240
           VEGHYVPNAGF EDVV+ DP+L+S+ Q  DSCEG    +AS+  QND SNK+ G SVD  
Sbjct: 181 VEGHYVPNAGFLEDVVFEDPSLSSACQASDSCEGVN--EASSDPQNDISNKKEGESVD-- 240

Query: 241 TSDVLDEPGVVLTAQRDSTTD---DIVSGVNDLKVVNDISAEEPNVQHTLSAEDVDKLLD 300
             D+  E     TAQ D + D    I + V+DLKV ++ S  E  V HTLS EDVD LLD
Sbjct: 241 VLDLTSEANPSSTAQLDVSNDPAEQITANVDDLKVTDEESNVE--VHHTLSTEDVDALLD 300

Query: 301 KCLLQALHTTVKDKDFPLPGSTLCSGGRLEHGGGVEQVLTATTSVPVRRTGKASFELQLL 360
           KCLLQALHTTVKDKD P+PGSTL +G           VL    S       K+S+  + L
Sbjct: 301 KCLLQALHTTVKDKDLPMPGSTLWAG----------HVLPCRPSGITLDIKKSSY--KKL 360

Query: 361 FREVHDKLGDDFI-VKEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHGKKES 420
            + +  K     I VKEDKHKKE VL SVNR HP+YTSF+PEK+QVEK DQ+++H   ES
Sbjct: 361 SKWLQAKSSTTLISVKEDKHKKEVVLNSVNRNHPEYTSFRPEKRQVEKSDQTSDHAASES 420

Query: 421 SSNKMLEVAEIYKPSVHVNPIFLAVGADASKLYTAAEATDIVFQYVEKEKLEKPTEKSRV 480
            S+K LEVAEIYKPSVHVNPIF +VG D  KL++A+EATDIVF+Y+EKE L KPT+KS V
Sbjct: 421 RSHKTLEVAEIYKPSVHVNPIFASVGVDTGKLFSASEATDIVFRYIEKENLVKPTDKSMV 480

Query: 481 VLDAILCDALFKGAIKKGTTYPTEIHKKDIGSTFISRMQPHHIVTRGSESVVRKGALKTI 540
           VLDAILCDALFKGAIKKG+TYPT IHKK++G TF++RMQ HH+VTRG ESVVRKGALK I
Sbjct: 481 VLDAILCDALFKGAIKKGSTYPTAIHKKELGHTFVNRMQAHHVVTRGGESVVRKGALKNI 540

Query: 541 QIMTERRQGNKKVTRLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVVQGGV 600
           QIMTERRQGNKKVT++SG E+FL+DAEALASELQKK ACSTTVAELPGKKG+EV+VQGGV
Sbjct: 541 QIMTERRQGNKKVTKVSGFESFLMDAEALASELQKKFACSTTVAELPGKKGHEVLVQGGV 600

Query: 601 IDDLARHLIEQYGIPKKYIEVLDKTRK 624
           I+DLA+HL+EQYGIPK+ IEVLDKT+K
Sbjct: 601 IEDLAKHLVEQYGIPKRCIEVLDKTKK 609

BLAST of Lsi03G004720 vs. NCBI nr
Match: gi|743921569|ref|XP_011004850.1| (PREDICTED: eukaryotic translation initiation factor 2D [Populus euphratica])

HSP 1 Score: 802.0 bits (2070), Expect = 7.5e-229
Identity = 422/631 (66.88%), Postives = 494/631 (78.29%), Query Frame = 1

Query: 1   MFKKAAEAKLHQRLSGADRKKLKRTVKERFPGASDADIDVLLPPKVEFSVAKFQNRVHIY 60
           MFKKA EAK HQRLSGADRKKLKRT+++RF  ASDADID LLPPK E +V+KF NRVH+Y
Sbjct: 1   MFKKAIEAKSHQRLSGADRKKLKRTIRDRFHRASDADIDALLPPKAEITVSKFVNRVHLY 60

Query: 61  SLEGGFPMFFDVDGRGTEIFPTVFALWMVPELLPAFVLKGGEVSRFVIGGADLMFPGIQV 120
            +EG FPMFFDVDGRG+EIFPTVFALW VPE+LP+F+LKGGEVSRFVIGGADLMFPGI +
Sbjct: 61  GVEGSFPMFFDVDGRGSEIFPTVFALWKVPEMLPSFILKGGEVSRFVIGGADLMFPGISI 120

Query: 121 PAEGLPSFLSGVPWAVKVPGNASPIAVGVTTMSSTEALKAGLRGKALRILHYYRDSLWGS 180
           PAEGLPSFL G PWAVKVPGN +PIAVG TTMS+ EALKAGLRGKALRI HYYRD LW S
Sbjct: 121 PAEGLPSFLEGEPWAVKVPGNPAPIAVGSTTMSTAEALKAGLRGKALRITHYYRDLLWES 180

Query: 181 VEGHYVPNAGFFEDVVYGDPALASSSQTDDSCEGTETADASNYGQNDSSNKEVGGSVDET 240
           VEGHYVPNAGF EDVV+ DP   SS+Q  DSCEG  + D+SN+  +   +KEVG S D  
Sbjct: 181 VEGHYVPNAGFLEDVVFEDPVFLSSAQVPDSCEG--SGDSSNHENDGVDSKEVGESAD-- 240

Query: 241 TSDVLDEPGVVLTAQRDSTTD---DIVSGVNDLKVVNDISAEEPNV----QHTLSAEDVD 300
             D + +       Q DS TD    +   +   KV ++I+A+E       QHTLSAED+D
Sbjct: 241 VKDPISDSHPTSKTQLDSDTDIVEQVTKAIGGFKVTDNIAADESTTVVEEQHTLSAEDLD 300

Query: 301 KLLDKCLLQALHTTVKDKDFPLPGSTLCSGGRLEHGGGVEQVLTATTSVPVRRTGKASFE 360
             LDKCLLQALHTTV DKD P+PGSTL S            VL    S       K+S  
Sbjct: 301 AYLDKCLLQALHTTVNDKDLPMPGSTLWS----------NHVLPCRPSGITLDIKKSSH- 360

Query: 361 LQLLFREVHDKLGDDFI-VKEDKHKKETVLYSVNRKHPDYTSFKPEKQQVEKIDQSNNHG 420
            + L + +  K     I VKEDK+KKETVL+SVNR HPDY SFKPE++Q +K+DQ  +  
Sbjct: 361 -KKLSKWLQAKSSSGLISVKEDKYKKETVLFSVNRSHPDYLSFKPERRQEQKVDQGGDQA 420

Query: 421 KKESSSNKMLEVAEIYKPSVHVNPIFLAVGADASKLYTAAEATDIVFQYVEKEKLEKPTE 480
            KE+ S K+LEV+EIYKPSVHVNPIF  VGAD  KLY+A+EA+D+VF+Y+EK  L KP  
Sbjct: 421 VKETRSPKLLEVSEIYKPSVHVNPIFAFVGADTGKLYSASEASDVVFKYIEKGHLVKPMN 480

Query: 481 KSRVVLDAILCDALFKGAIKKGTTYPTEIHKKDIGSTFISRMQPHHIVTRGSESVVRKGA 540
           KS VVLD  LCDALFKGAIKKG+ YPTEIHKKD+GSTF+SRMQ H+IVTRGSESVVRKG+
Sbjct: 481 KSIVVLDPTLCDALFKGAIKKGSAYPTEIHKKDLGSTFVSRMQAHYIVTRGSESVVRKGS 540

Query: 541 LKTIQIMTERRQGNKKVTRLSGLETFLLDAEALASELQKKCACSTTVAELPGKKGYEVVV 600
           ++TIQI+TERRQGNKKVT+LSG+ETFL+DA+ALASELQKK ACST+V+ELPGKKG+EV++
Sbjct: 541 VRTIQILTERRQGNKKVTKLSGMETFLMDADALASELQKKFACSTSVSELPGKKGHEVLI 600

Query: 601 QGGVIDDLARHLIEQYGIPKKYIEVLDKTRK 624
           QGGVIDDLARHL+EQYG+PK+YIEVLDKT+K
Sbjct: 601 QGGVIDDLARHLVEQYGVPKRYIEVLDKTKK 615