Lsi03G004710 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi03G004710
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionAcetyl esterase
Locationchr03 : 5337750 .. 5338748 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTCCATTGGCGACACACAATCTCCTTCTTCACCTCCCAAACTTCCAGGGAAGCGTAAGCTTGCCAATCTGTTCACTTTCATCCTTTTCCGACAAGCATTCCGATCAGATTTTACGGTCAACCGCCTCCTCCTCCGCCTTCTTGACCGTAAAGTACCTCCTTCCACCTCTCGCGGCGTCGCCGCCTTTGACACGACCGTCGATTCCTCCTCTTCCGACCTTTGGATTCGTGTTTATAATCCTCTAACATTTTCCGACTCCGATCCTTTGCCTATTATCATATACTTCCACGGCGGGGGATTTGTTTTTGGCTCCGCTGATTCTCCGTCCATCGATACCTTCTGTCGCGATTTTGCTCGCGAAATCGGAGCCATTGTCATCTCTGTCAACTACCGCCTCGCGCCGGAACACCGATTTCCGAGCCAATTCGACGACGGATTTCAAGTTCTTAAGGCAATAGACGAAGGAGTGATCTCTGAAACTCTTCCTGAAAACGCCGATCTGAGCCGCTGTTTCATCGCCGGCGAAAGCGCCGGAGGAAACATAGCTCATCACGTGATGGTGAGAGCGGCGGAGTATCAATTCAAGAGGCTTAAGATTGCAGGCCTGATTTTGATTCAGCCATTTTTGGGAGGAGAAGAGCGGAGGGATTCAGAGCTTCGATTTGGGAGCGGCTATGGACTGACTTTGGAAATGGCCGATTGGTATTGGAAGGCGTGGCTGTCGATCGGTTCGAACCGGGATCACCCGGCCGCCAACATGGTTCGGTCGAACATTTCGGGCCTGAAGTTTCCACCGACGCTCGTTGTGATTGGGGGTTTGGATTTGTTGAGAGATCGGAACAAAGAGTACAGTAACTGGTTGGAGAAATCGGGTCAAGAAGTTCGAGTCGTGGAGTATCCGAATGGGACCCATGGGTTTAGTTGGAACTCGGATCTTCCCGAATATTCCATGCTGATGCAGCACGCCAAAGAATTTACCAAACAAATTTCTTAG

mRNA sequence

ATGTCTTCCATTGGCGACACACAATCTCCTTCTTCACCTCCCAAACTTCCAGGGAAGCGTAAGCTTGCCAATCTGTTCACTTTCATCCTTTTCCGACAAGCATTCCGATCAGATTTTACGGTCAACCGCCTCCTCCTCCGCCTTCTTGACCGTAAAGTACCTCCTTCCACCTCTCGCGGCGTCGCCGCCTTTGACACGACCGTCGATTCCTCCTCTTCCGACCTTTGGATTCGTGTTTATAATCCTCTAACATTTTCCGACTCCGATCCTTTGCCTATTATCATATACTTCCACGGCGGGGGATTTGTTTTTGGCTCCGCTGATTCTCCGTCCATCGATACCTTCTGTCGCGATTTTGCTCGCGAAATCGGAGCCATTGTCATCTCTGTCAACTACCGCCTCGCGCCGGAACACCGATTTCCGAGCCAATTCGACGACGGATTTCAAGTTCTTAAGGCAATAGACGAAGGAGTGATCTCTGAAACTCTTCCTGAAAACGCCGATCTGAGCCGCTGTTTCATCGCCGGCGAAAGCGCCGGAGGAAACATAGCTCATCACGTGATGGTGAGAGCGGCGGAGTATCAATTCAAGAGGCTTAAGATTGCAGGCCTGATTTTGATTCAGCCATTTTTGGGAGGAGAAGAGCGGAGGGATTCAGAGCTTCGATTTGGGAGCGGCTATGGACTGACTTTGGAAATGGCCGATTGGTATTGGAAGGCGTGGCTGTCGATCGGTTCGAACCGGGATCACCCGGCCGCCAACATGGTTCGGTCGAACATTTCGGGCCTGAAGTTTCCACCGACGCTCGTTGTGATTGGGGGTTTGGATTTGTTGAGAGATCGGAACAAAGAGTACAGTAACTGGTTGGAGAAATCGGGTCAAGAAGTTCGAGTCGTGGAGTATCCGAATGGGACCCATGGGTTTAGTTGGAACTCGGATCTTCCCGAATATTCCATGCTGATGCAGCACGCCAAAGAATTTACCAAACAAATTTCTTAG

Coding sequence (CDS)

ATGTCTTCCATTGGCGACACACAATCTCCTTCTTCACCTCCCAAACTTCCAGGGAAGCGTAAGCTTGCCAATCTGTTCACTTTCATCCTTTTCCGACAAGCATTCCGATCAGATTTTACGGTCAACCGCCTCCTCCTCCGCCTTCTTGACCGTAAAGTACCTCCTTCCACCTCTCGCGGCGTCGCCGCCTTTGACACGACCGTCGATTCCTCCTCTTCCGACCTTTGGATTCGTGTTTATAATCCTCTAACATTTTCCGACTCCGATCCTTTGCCTATTATCATATACTTCCACGGCGGGGGATTTGTTTTTGGCTCCGCTGATTCTCCGTCCATCGATACCTTCTGTCGCGATTTTGCTCGCGAAATCGGAGCCATTGTCATCTCTGTCAACTACCGCCTCGCGCCGGAACACCGATTTCCGAGCCAATTCGACGACGGATTTCAAGTTCTTAAGGCAATAGACGAAGGAGTGATCTCTGAAACTCTTCCTGAAAACGCCGATCTGAGCCGCTGTTTCATCGCCGGCGAAAGCGCCGGAGGAAACATAGCTCATCACGTGATGGTGAGAGCGGCGGAGTATCAATTCAAGAGGCTTAAGATTGCAGGCCTGATTTTGATTCAGCCATTTTTGGGAGGAGAAGAGCGGAGGGATTCAGAGCTTCGATTTGGGAGCGGCTATGGACTGACTTTGGAAATGGCCGATTGGTATTGGAAGGCGTGGCTGTCGATCGGTTCGAACCGGGATCACCCGGCCGCCAACATGGTTCGGTCGAACATTTCGGGCCTGAAGTTTCCACCGACGCTCGTTGTGATTGGGGGTTTGGATTTGTTGAGAGATCGGAACAAAGAGTACAGTAACTGGTTGGAGAAATCGGGTCAAGAAGTTCGAGTCGTGGAGTATCCGAATGGGACCCATGGGTTTAGTTGGAACTCGGATCTTCCCGAATATTCCATGCTGATGCAGCACGCCAAAGAATTTACCAAACAAATTTCTTAG

Protein sequence

MSSIGDTQSPSSPPKLPGKRKLANLFTFILFRQAFRSDFTVNRLLLRLLDRKVPPSTSRGVAAFDTTVDSSSSDLWIRVYNPLTFSDSDPLPIIIYFHGGGFVFGSADSPSIDTFCRDFAREIGAIVISVNYRLAPEHRFPSQFDDGFQVLKAIDEGVISETLPENADLSRCFIAGESAGGNIAHHVMVRAAEYQFKRLKIAGLILIQPFLGGEERRDSELRFGSGYGLTLEMADWYWKAWLSIGSNRDHPAANMVRSNISGLKFPPTLVVIGGLDLLRDRNKEYSNWLEKSGQEVRVVEYPNGTHGFSWNSDLPEYSMLMQHAKEFTKQIS
Homology
[Close]
BLAST of Lsi03G004710 vs. Swiss-Prot
Match: CXE18_ARATH (Probable carboxylesterase 18 OS=Arabidopsis thaliana GN=CXE18 PE=1 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 4.5e-71
Identity = 147/329 (44.68%), Postives = 196/329 (59.57%), Query Frame = 1 

Query: 10  PSSPPKLPGKRKLANLFTFILFRQAFRSDFTVNRLLLRLLDRKVPPSTS--RGVAAFDTT 69
           P+    LP K ++A      +   A R D T+NR  LRL D + PP+      V+  D  
Sbjct: 7   PNQKLTLPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFV 66

Query: 70  VDSSSSDLWIRVYNPLTFSDSDPLPIIIYFHGGGFVFGSADSPSIDTFCRDFAREIGAIV 129
           VD S  DLW R+Y P      D +P++++FHGGGF F S ++   D  CR FAR++ A V
Sbjct: 67  VDQSR-DLWFRLYTPHV--SGDKIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYV 126

Query: 130 ISVNYRLAPEHRFPSQFDDGFQVLKAIDEGVISETLPENADLSRCFIAGESAGGNIAHHV 189
           ISVNYRLAPEHR+P+Q+DDGF  LK I+E   S  LP NADLSRCF AG+SAGGNIAH+V
Sbjct: 127 ISVNYRLAPEHRYPAQYDDGFDALKYIEENHGS-ILPANADLSRCFFAGDSAGGNIAHNV 186

Query: 190 MVRAAEY---QFKRLKIAGLILIQPFLGGEERRDSELRFGSGYGLTLEMADWYWKAWLSI 249
            +R        F  +K+ GLI IQPF GGEER ++E +      ++ +  DW WKA   +
Sbjct: 187 AIRICREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKA---M 246

Query: 250 GSNRDHPAANMVRSN---ISGLKFPPTLVVIGGLDLLRDRNKEYSNWLEKSGQEVRVVEY 309
           G NRDH A N+   N   ISGL +P T+VV+ G D L+D  + Y  WL+  G++  ++EY
Sbjct: 247 GLNRDHEAVNVGGPNAVDISGLDYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEY 306

Query: 310 PNGTHGFSWNSDLPEYSMLMQHAKEFTKQ 331
           PN  H F    +LPE   L+   K+F  +
Sbjct: 307 PNMFHAFYIFPELPEAGQLIMRIKDFVDE 328

[Close]
BLAST of Lsi03G004710 vs. Swiss-Prot
Match: GID1C_ARATH (Gibberellin receptor GID1C OS=Arabidopsis thaliana GN=GID1C PE=1 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 1.2e-55
Identity = 121/291 (41.58%), Postives = 169/291 (58.08%), Query Frame = 1 

Query: 36  RSDFTVNRLLLRLLDRKVPPSTS--RGVAAFDTTVDSSSSDLWIRVYNPLTFS------- 95
           R D T NR L   LDRKVP + +   GV +FD  +D  ++ L  RVY P           
Sbjct: 36  RPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTN-LLSRVYRPADAGTSPSITD 95

Query: 96  -----DSDPLPIIIYFHGGGFVFGSADSPSIDTFCRDFAREIGAIVISVNYRLAPEHRFP 155
                D + +P+I++FHGG F   SA+S   DT CR      GA+V+SVNYR APE+R+P
Sbjct: 96  LQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRRAPENRYP 155

Query: 156 SQFDDGFQVLKAIDEGVISETLPENADLS-RCFIAGESAGGNIAHHVMVRAAEYQFKRLK 215
             +DDG+ VLK ++    S  L    D   R F+AG+S+GGNI H+V VRA E    R+ 
Sbjct: 156 CAYDDGWAVLKWVNS---SSWLRSKKDSKVRIFLAGDSSGGNIVHNVAVRAVE---SRID 215

Query: 216 IAGLILIQPFLGGEERRDSELRFGSGYGLTLEMADWYWKAWLSIGSNRDHPAANMV---R 275
           + G IL+ P  GG ER +SE R    Y +T+   DWYW+A+L  G +R+HPA +      
Sbjct: 216 VLGNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRAFLPEGEDREHPACSPFGPRS 275

Query: 276 SNISGLKFPPTLVVIGGLDLLRDRNKEYSNWLEKSGQEVRVVEYPNGTHGF 309
            ++ GL FP +LVV+ GLDL++D   +Y+  L+K+GQEV+++     T GF
Sbjct: 276 KSLEGLSFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGF 319

[Close]
BLAST of Lsi03G004710 vs. Swiss-Prot
Match: GID1_ORYSJ (Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica GN=GID1 PE=1 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 7.7e-55
Identity = 123/320 (38.44%), Postives = 175/320 (54.69%), Query Frame = 1 

Query: 36  RSDFTVNRLLLRLLDRKVPPSTS--RGVAAFDTTVDSSSSDLWIRVYNPLTFSDSD---- 95
           R+D T  R L   LDR+VP +     GV++FD  +D S   L +R+Y      D++    
Sbjct: 36  RADGTFERDLGEYLDRRVPANARPLEGVSSFDHIIDQSVG-LEVRIYRAAAEGDAEEGAA 95

Query: 96  -----------------PLPIIIYFHGGGFVFGSADSPSIDTFCRDFAREIGAIVISVNY 155
                            P P+II+FHGG FV  SA S   D+ CR F +    +V+SVNY
Sbjct: 96  AVTRPILEFLTDAPAAEPFPVIIFFHGGSFVHSSASSTIYDSLCRRFVKLSKGVVVSVNY 155

Query: 156 RLAPEHRFPSQFDDGFQVLKAIDEGVISETLPENAD--LSRCFIAGESAGGNIAHHVMVR 215
           R APEHR+P  +DDG+  LK     V+S+    +     +R F++G+S+GGNIAHHV VR
Sbjct: 156 RRAPEHRYPCAYDDGWTALK----WVMSQPFMRSGGDAQARVFLSGDSSGGNIAHHVAVR 215

Query: 216 AAEYQFKRLKIAGLILIQPFLGGEERRDSELRFGSGYGLTLEMADWYWKAWLSIGSNRDH 275
           AA+   + +K+ G IL+    GG ER +SE R    Y +TL+  DWYWKA+L   ++RDH
Sbjct: 216 AAD---EGVKVCGNILLNAMFGGTERTESERRLDGKYFVTLQDRDWYWKAYLPEDADRDH 275

Query: 276 PAANMVRSN---ISGLKFPPTLVVIGGLDLLRDRNKEYSNWLEKSGQEVRVVEYPNGTHG 328
           PA N    N   + GL F  +L+++ GLDL  DR   Y++ L + G  V+VV+  N T G
Sbjct: 276 PACNPFGPNGRRLGGLPFAKSLIIVSGLDLTCDRQLAYADALREDGHHVKVVQCENATVG 335

[Close]
BLAST of Lsi03G004710 vs. Swiss-Prot
Match: GID1B_ARATH (Gibberellin receptor GID1B OS=Arabidopsis thaliana GN=GID1B PE=1 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 3.6e-52
Identity = 120/339 (35.40%), Postives = 188/339 (55.46%), Query Frame = 1 

Query: 20  RKLANLFTFIL---FRQAF----RSDFTVNRLLLRLLDRKVPPSTSR--GVAAFDTTVDS 79
           +++  L T++L   F+ A+    R D + NR L   LDRKVP ++    GV +FD     
Sbjct: 13  KRIVPLNTWVLISNFKLAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFDHV--D 72

Query: 80  SSSDLWIRVYNPLTF---------------SDSDPLPIIIYFHGGGFVFGSADSPSIDTF 139
           S+++L  R+Y P +                S ++ +P++I+FHGG F   SA+S   DTF
Sbjct: 73  STTNLLTRIYQPASLLHQTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTF 132

Query: 140 CRDFAREIGAIVISVNYRLAPEHRFPSQFDDGFQVLKAIDEGVISETLPENADLSRCFIA 199
           CR      G +V+SV+YR +PEHR+P  +DDG+  L  +   V  ++  ++      ++A
Sbjct: 133 CRRLVTICGVVVVSVDYRRSPEHRYPCAYDDGWNALNWVKSRVWLQSGKDSN--VYVYLA 192

Query: 200 GESAGGNIAHHVMVRAAEYQFKRLKIAGLILIQPFLGGEERRDSELRFGSGYGLTLEMAD 259
           G+S+GGNIAH+V VRA     + +K+ G IL+ P  GG+ER  SE      Y +T++  D
Sbjct: 193 GDSSGGNIAHNVAVRATN---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRD 252

Query: 260 WYWKAWLSIGSNRDHPAANMV---RSNISGLKFPPTLVVIGGLDLLRDRNKEYSNWLEKS 319
           WYW+A+L  G +RDHPA N       ++ G+ FP +LVV+ GLDL++D    Y + L+K+
Sbjct: 253 WYWRAYLPEGEDRDHPACNPFGPRGQSLKGVNFPKSLVVVAGLDLVQDWQLAYVDGLKKT 312

Query: 320 GQEVRVVEYPNGTHGFSWNSDLPEYSMLMQHAKEFTKQI 332
           G EV ++     T GF +  +   +  LM+   +F   I
Sbjct: 313 GLEVNLLYLKQATIGFYFLPNNDHFHCLMEELNKFVHSI 344

[Close]
BLAST of Lsi03G004710 vs. Swiss-Prot
Match: GID1A_ARATH (Gibberellin receptor GID1A OS=Arabidopsis thaliana GN=GID1A PE=1 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 2.8e-49
Identity = 118/315 (37.46%), Postives = 171/315 (54.29%), Query Frame = 1 

Query: 20  RKLANLFTFIL---FRQAF----RSDFTVNRLLLRLLDRKVPPSTSR--GVAAFDTTVDS 79
           R +  L T++L   F+ A+    R D T NR L   LDRKV  + +   GV +FD  +D 
Sbjct: 13  RTVVPLNTWVLISNFKVAYNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDR 72

Query: 80  SSSDLWIRVYNPLTFS--------------DSDPLPIIIYFHGGGFVFGSADSPSIDTFC 139
             + L  RVY P                  D D +P+I++FHGG F   SA+S   DT C
Sbjct: 73  RIN-LLSRVYRPAYADQEQPPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLC 132

Query: 140 RDFAREIGAIVISVNYRLAPEHRFPSQFDDGFQVLKAIDEGVISETLPENADLSRCFIAG 199
           R        +V+SVNYR APE+ +P  +DDG+  L  ++     ++  ++      F+AG
Sbjct: 133 RRLVGLCKCVVVSVNYRRAPENPYPCAYDDGWIALNWVNSRSWLKSKKDSK--VHIFLAG 192

Query: 200 ESAGGNIAHHVMVRAAEYQFKRLKIAGLILIQPFLGGEERRDSELRFGSGYGLTLEMADW 259
           +S+GGNIAH+V +RA E     + + G IL+ P  GG ER +SE      Y +T+   DW
Sbjct: 193 DSSGGNIAHNVALRAGE---SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDW 252

Query: 260 YWKAWLSIGSNRDHPAANMVR---SNISGLKFPPTLVVIGGLDLLRDRNKEYSNWLEKSG 309
           YWKA+L  G +R+HPA N       ++ G+ FP +LVV+ GLDL+RD    Y+  L+K+G
Sbjct: 253 YWKAFLPEGEDREHPACNPFSPRGKSLEGVSFPKSLVVVAGLDLIRDWQLAYAEGLKKAG 312

[Close]
BLAST of Lsi03G004710 vs. TrEMBL
Match: A0A0A0LMQ6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G401430 PE=4 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 1.5e-153
Identity = 266/333 (79.88%), Postives = 292/333 (87.69%), Query Frame = 1 

Query: 1   MSSIGDTQSP-SSPPKLPGKRKLANLFTFILFRQAFRSDFTVNRLLLRLLDRKVPPSTSR 60
           M+S GD QSP SS P LP KRKLA+LFT  LFRQAFRSDFTVNR  LRL DRK+PP TSR
Sbjct: 1   MASTGDAQSPNSSLPTLPWKRKLADLFTSFLFRQAFRSDFTVNRRFLRLFDRKLPPFTSR 60

Query: 61  GVAAFDTTVDSSSSDLWIRVYNPLTFSDSDPLPIIIYFHGGGFVFGSADSPSIDTFCRDF 120
           GVAA D T+DSS+SDLWIRVYNPLTFS+SDPLP+IIYFHGGGFV+GSAD+P  DTFCRDF
Sbjct: 61  GVAASDATIDSSTSDLWIRVYNPLTFSNSDPLPVIIYFHGGGFVYGSADAPPTDTFCRDF 120

Query: 121 AREIGAIVISVNYRLAPEHRFPSQFDDGFQVLKAIDEGVISETLPENADLSRCFIAGESA 180
           AREIGAIVISVNYRLAPE RFPSQFDDGF VLKA+D+G ISET+PENADL RCFIAGESA
Sbjct: 121 AREIGAIVISVNYRLAPEDRFPSQFDDGFHVLKAMDKGAISETVPENADLRRCFIAGESA 180

Query: 181 GGNIAHHVMVRAAEYQFKRLKIAGLILIQPFLGGEERRDSELRFGSGYGLTLEMADWYWK 240
           GGNIAHHV VRAAE +FKR+KI G+ILIQPF GGEERRDSE+RFG GYGLTLEM DW+WK
Sbjct: 181 GGNIAHHVTVRAAESEFKRVKIVGMILIQPFFGGEERRDSEIRFGRGYGLTLEMTDWFWK 240

Query: 241 AWLSIGSNRDHPAANMVRSNISGLKFPPTLVVIGGLDLLRDRNKEYSNWLEKSGQEVRVV 300
           AWL +GSNRDH AAN+V S+ISG+K P  LVVIGGLDLLRDRN+EY  WL+KSGQEVRVV
Sbjct: 241 AWLPVGSNRDHTAANVVGSSISGVKVPAALVVIGGLDLLRDRNREYVEWLKKSGQEVRVV 300

Query: 301 EYPNGTHGFSWNSDLPEYSMLMQHAKEFTKQIS 333
           EYPNGTHGF    DLPEYSML+Q AK+F  +IS
Sbjct: 301 EYPNGTHGFIGKPDLPEYSMLIQDAKQFINKIS 333

[Close]
BLAST of Lsi03G004710 vs. TrEMBL
Match: A5Y726_CUCME (Esterase OS=Cucumis melo PE=4 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 3.0e-90
Identity = 169/324 (52.16%), Postives = 226/324 (69.75%), Query Frame = 1 

Query: 12  SPPKLPGKRKLANLFTFILFRQAFRSDFTVNRLLLRLLDRKVPPSTSR-GVAAFDTTVDS 71
           +P KLP K +L       +  +  R + TVNR L+ L + KV  S+ R GV   DT +D 
Sbjct: 10  NPQKLPLKYRLILPLLSFVSHKCLRPNMTVNRSLISLFESKVSSSSPRDGVFTCDTVIDP 69

Query: 72  SSSDLWIRVYNPLTFSDSDPLPIIIYFHGGGFVFGSADSPSIDTFCRDFAREIGAIVISV 131
           S + LW R++ P +     P+P++IYFHGGGFVF S D  S DT CR  ARE+ AIV+SV
Sbjct: 70  SRN-LWFRLFVPSSTPHDLPIPLLIYFHGGGFVFFSPDFLSFDTLCRKLARELQAIVVSV 129

Query: 132 NYRLAPEHRFPSQFDDGFQVLKAIDEGVISETLPENADLSRCFIAGESAGGNIAHHVMVR 191
           NYRL+PEHR+PSQ++DGF  LK ID+ + S   P+ +D  RCFIAG+SAGGNIAHHV+VR
Sbjct: 130 NYRLSPEHRYPSQYEDGFDALKFIDD-LDSSAFPKKSDFGRCFIAGDSAGGNIAHHVVVR 189

Query: 192 AAEYQFKRLKIAGLILIQPFLGGEERRDSELRFGSGYGLTLEMADWYWKAWLSIGSNRDH 251
           +++Y FK++KI GLI IQPF GGEER +SE+RFG    L LE ADWYWKA+L  G+NR+H
Sbjct: 190 SSDYNFKKVKIRGLIAIQPFFGGEERTESEIRFGRSPTLNLERADWYWKAFLPDGANRNH 249

Query: 252 PAANMVRS---NISGLKFPPTLVVIGGLDLLRDRNKEYSNWLEKSGQEVRVVEYPNGTHG 311
            AA++      NIS +KFP TLV++GG D LRD +++Y  WL+K+G+EV +VEYP   HG
Sbjct: 250 GAAHVFGEDGVNISAVKFPATLVIVGGSDQLRDWDRKYYEWLKKAGKEVELVEYPKAIHG 309

Query: 312 FSWNSDLPEYSMLMQHAKEFTKQI 332
           F   S+LPE  +L++ AK F +++
Sbjct: 310 FYVISELPETWLLIEEAKNFIEKV 331

[Close]
BLAST of Lsi03G004710 vs. TrEMBL
Match: A0A0A0LUJ2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G466560 PE=4 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 4.3e-89
Identity = 168/325 (51.69%), Postives = 224/325 (68.92%), Query Frame = 1 

Query: 12  SPPKLPGKRKLANLFTFILFRQAFRSDFTVNRLLLRLLDRKVPPSTSR--GVAAFDTTVD 71
           +P KLP K +L       +  +A R + TVNR L+ L + KV  S++   GV   DT +D
Sbjct: 10  NPQKLPLKYRLLLRLRSFVSHKALRPNMTVNRSLISLFESKVSSSSTPRDGVFTCDTVID 69

Query: 72  SSSSDLWIRVYNPLTFSDSDPLPIIIYFHGGGFVFGSADSPSIDTFCRDFAREIGAIVIS 131
            S + LW R++ P +     P+P+++YFHGGGFVF S DS   D  CR  ARE+ A+V+S
Sbjct: 70  PSRN-LWFRLFVPSSTPHDLPIPLLVYFHGGGFVFFSPDSLPFDILCRKLARELQAVVVS 129

Query: 132 VNYRLAPEHRFPSQFDDGFQVLKAIDEGVISETLPENADLSRCFIAGESAGGNIAHHVMV 191
           VNYRL+PEHR+PSQ++DGF  LK ID+ + S   PE +D SRCFIAG+SAGGNIAHHV+V
Sbjct: 130 VNYRLSPEHRYPSQYEDGFDALKFIDD-LDSSAFPEKSDFSRCFIAGDSAGGNIAHHVIV 189

Query: 192 RAAEYQFKRLKIAGLILIQPFLGGEERRDSELRFGSGYGLTLEMADWYWKAWLSIGSNRD 251
           R+++Y FK++KI GLI IQPF GGEER +SE+RFG    L LE ADWYWKA+L  G+NR+
Sbjct: 190 RSSDYNFKKVKIRGLIAIQPFFGGEERTESEIRFGETPTLNLERADWYWKAFLPDGANRN 249

Query: 252 HPAANMVRS---NISGLKFPPTLVVIGGLDLLRDRNKEYSNWLEKSGQEVRVVEYPNGTH 311
           H AA++       ISG+K P TLV++GG D LRD +++Y  WL+K G+EV +VEY N  H
Sbjct: 250 HVAAHVFGEKGVKISGVKLPATLVIVGGSDQLRDWDRKYYEWLKKGGKEVEMVEYANAIH 309

Query: 312 GFSWNSDLPEYSMLMQHAKEFTKQI 332
           GF    +LPE S+L++ AK F  +I
Sbjct: 310 GFYAIPELPETSLLIEEAKNFIGRI 332

[Close]
BLAST of Lsi03G004710 vs. TrEMBL
Match: A0A0A0KXS8_CUCSA (Acetyl esterase OS=Cucumis sativus GN=Csa_5G647390 PE=4 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 2.0e-86
Identity = 171/330 (51.82%), Postives = 224/330 (67.88%), Query Frame = 1 

Query: 12  SPPKLPGKRKLANLFTFILFRQAFRSDFTVNRLLLRLLDRKVPPS--TSRGVAAFDTTVD 71
           S P+LP K K+   F  +LF  + RSDFTVNR LL  LD K+PP+  ++  V++ D T+D
Sbjct: 11  STPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAHSVSSSDLTID 70

Query: 72  SSSSDLWIRVYNP---LTFSDSDPL-PIIIYFHGGGFVFGSADSPSIDTFCRDFAREIGA 131
           +S  DL++R++ P       +S PL PII YFHGGGF FGSAD+ S D   R FA ++ A
Sbjct: 71  TSR-DLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAARGFAEKLRA 130

Query: 132 IVISVNYRLAPEHRFPSQFDDGFQVLKAIDEGVISETLPENADLSRCFIAGESAGGNIAH 191
           +VISVNYRLAPE RFP Q+DDGF  LK IDE +  ++L E  DLSRCFI GESAGGN+ H
Sbjct: 131 VVISVNYRLAPEFRFPCQYDDGFDALKFIDE-MDDDSLLERVDLSRCFILGESAGGNLGH 190

Query: 192 HVMVRAAEYQFKRLKIAGLILIQPFLGGEERRDSELRFGSGYGLTLEMADWYWKAWLSIG 251
           HV VRA+EY+FKR+KI G I  QPF GG+ER +SE R      LTL M DW+W+A+L  G
Sbjct: 191 HVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWFWRAFLPAG 250

Query: 252 SNRDHPAANMVRSN---ISGLK-FPPTLVVIGGLDLLRDRNKEYSNWLEKSGQEVRVVEY 311
            +RDH AAN+   N   ISGL+ FP T++  GGLDLL DR K Y   L++ G++V++V +
Sbjct: 251 EDRDHAAANVNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMGKDVKLVVF 310

Query: 312 PNGTHGFSWNSDLPEYSMLMQHAKEFTKQI 332
            N  HGF    DLPEYS++++   +F  ++
Sbjct: 311 SNAFHGFFGFPDLPEYSLMIEEMSDFIAKL 338

[Close]
BLAST of Lsi03G004710 vs. TrEMBL
Match: A0A0A0KUP3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G647370 PE=4 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 1.2e-83
Identity = 166/334 (49.70%), Postives = 218/334 (65.27%), Query Frame = 1 

Query: 3   SIGDTQSPSSPPKLPGKRKLANLFTFILFRQAFRSDFTVNRLLLRLLDRKVPPSTSR--G 62
           S   + S  S  KLP K +L       +     RSDFTVNR    +LD K+PPST    G
Sbjct: 2   SSSTSASEISSSKLPWKHRLLLRIGSTITDACCRSDFTVNRWFTGILDWKIPPSTKPIDG 61

Query: 63  VAAFDTTVDSSSSDLWIRVYNPLTF---SDSDPLPIIIYFHGGGFVFGSADSPSIDTFCR 122
           V++FD T+D+S + LW+R++NP+     SD   LP+I YFHGGGF F  ADS    T   
Sbjct: 62  VSSFDLTIDTSRN-LWVRIFNPVIDGEDSDIQSLPLIFYFHGGGFAFSYADSALSHTSAH 121

Query: 123 DFAREIGAIVISVNYRLAPEHRFPSQFDDGFQVLKAIDEGVISETLPENADLSRCFIAGE 182
            FA+++ A+VISVNYRLAPE R+P Q+DDGF  LK IDE V  E LP  ADL+RCFI GE
Sbjct: 122 RFAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDE-VGEEILPAKADLTRCFILGE 181

Query: 183 SAGGNIAHHVMVRAAEYQFKRLKIAGLILIQPFLGGEERRDSELRFGSGYGLTLEMADWY 242
           SAGGN+ HHV VRA+EY  K++K+ G I  QPF GGEER +SE+R  +   L+L ++DW+
Sbjct: 182 SAGGNLGHHVAVRASEYTLKKVKMVGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWF 241

Query: 243 WKAWLSIGSNRDHPAANMV----RSNISGLKFPPTLVVIGGLDLLRDRNKEYSNWLEKSG 302
           WKA+L  G +RDH AAN+     R     +KFP TLV++G LDLL+D  + Y   L++ G
Sbjct: 242 WKAFLPEGEDRDHGAANVFGPKGRDVTEVMKFPATLVMVGELDLLQDGQRRYYEGLKRMG 301

Query: 303 QEVRVVEYPNGTHGFSWNSDLPEYSMLMQHAKEF 328
           +EV++VE+ N  HGF    DLP+YS +M+  K+F
Sbjct: 302 KEVKMVEFENAIHGFFAFWDLPQYSSMMKEMKDF 333

[Close]
BLAST of Lsi03G004710 vs. TAIR10
Match: AT5G23530.1 (AT5G23530.1 carboxyesterase 18)

HSP 1 Score: 269.6 bits (688), Expect = 2.5e-72
Identity = 147/329 (44.68%), Postives = 196/329 (59.57%), Query Frame = 1 

Query: 10  PSSPPKLPGKRKLANLFTFILFRQAFRSDFTVNRLLLRLLDRKVPPSTS--RGVAAFDTT 69
           P+    LP K ++A      +   A R D T+NR  LRL D + PP+      V+  D  
Sbjct: 7   PNQKLTLPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFV 66

Query: 70  VDSSSSDLWIRVYNPLTFSDSDPLPIIIYFHGGGFVFGSADSPSIDTFCRDFAREIGAIV 129
           VD S  DLW R+Y P      D +P++++FHGGGF F S ++   D  CR FAR++ A V
Sbjct: 67  VDQSR-DLWFRLYTPHV--SGDKIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYV 126

Query: 130 ISVNYRLAPEHRFPSQFDDGFQVLKAIDEGVISETLPENADLSRCFIAGESAGGNIAHHV 189
           ISVNYRLAPEHR+P+Q+DDGF  LK I+E   S  LP NADLSRCF AG+SAGGNIAH+V
Sbjct: 127 ISVNYRLAPEHRYPAQYDDGFDALKYIEENHGS-ILPANADLSRCFFAGDSAGGNIAHNV 186

Query: 190 MVRAAEY---QFKRLKIAGLILIQPFLGGEERRDSELRFGSGYGLTLEMADWYWKAWLSI 249
            +R        F  +K+ GLI IQPF GGEER ++E +      ++ +  DW WKA   +
Sbjct: 187 AIRICREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKA---M 246

Query: 250 GSNRDHPAANMVRSN---ISGLKFPPTLVVIGGLDLLRDRNKEYSNWLEKSGQEVRVVEY 309
           G NRDH A N+   N   ISGL +P T+VV+ G D L+D  + Y  WL+  G++  ++EY
Sbjct: 247 GLNRDHEAVNVGGPNAVDISGLDYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEY 306

Query: 310 PNGTHGFSWNSDLPEYSMLMQHAKEFTKQ 331
           PN  H F    +LPE   L+   K+F  +
Sbjct: 307 PNMFHAFYIFPELPEAGQLIMRIKDFVDE 328

[Close]
BLAST of Lsi03G004710 vs. TAIR10
Match: AT5G27320.1 (AT5G27320.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 218.4 bits (555), Expect = 6.7e-57
Identity = 121/291 (41.58%), Postives = 169/291 (58.08%), Query Frame = 1 

Query: 36  RSDFTVNRLLLRLLDRKVPPSTS--RGVAAFDTTVDSSSSDLWIRVYNPLTFS------- 95
           R D T NR L   LDRKVP + +   GV +FD  +D  ++ L  RVY P           
Sbjct: 36  RPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTN-LLSRVYRPADAGTSPSITD 95

Query: 96  -----DSDPLPIIIYFHGGGFVFGSADSPSIDTFCRDFAREIGAIVISVNYRLAPEHRFP 155
                D + +P+I++FHGG F   SA+S   DT CR      GA+V+SVNYR APE+R+P
Sbjct: 96  LQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRRAPENRYP 155

Query: 156 SQFDDGFQVLKAIDEGVISETLPENADLS-RCFIAGESAGGNIAHHVMVRAAEYQFKRLK 215
             +DDG+ VLK ++    S  L    D   R F+AG+S+GGNI H+V VRA E    R+ 
Sbjct: 156 CAYDDGWAVLKWVNS---SSWLRSKKDSKVRIFLAGDSSGGNIVHNVAVRAVE---SRID 215

Query: 216 IAGLILIQPFLGGEERRDSELRFGSGYGLTLEMADWYWKAWLSIGSNRDHPAANMV---R 275
           + G IL+ P  GG ER +SE R    Y +T+   DWYW+A+L  G +R+HPA +      
Sbjct: 216 VLGNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRAFLPEGEDREHPACSPFGPRS 275

Query: 276 SNISGLKFPPTLVVIGGLDLLRDRNKEYSNWLEKSGQEVRVVEYPNGTHGF 309
            ++ GL FP +LVV+ GLDL++D   +Y+  L+K+GQEV+++     T GF
Sbjct: 276 KSLEGLSFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGF 319

[Close]
BLAST of Lsi03G004710 vs. TAIR10
Match: AT3G63010.1 (AT3G63010.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 206.8 bits (525), Expect = 2.0e-53
Identity = 120/339 (35.40%), Postives = 188/339 (55.46%), Query Frame = 1 

Query: 20  RKLANLFTFIL---FRQAF----RSDFTVNRLLLRLLDRKVPPSTSR--GVAAFDTTVDS 79
           +++  L T++L   F+ A+    R D + NR L   LDRKVP ++    GV +FD     
Sbjct: 13  KRIVPLNTWVLISNFKLAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFDHV--D 72

Query: 80  SSSDLWIRVYNPLTF---------------SDSDPLPIIIYFHGGGFVFGSADSPSIDTF 139
           S+++L  R+Y P +                S ++ +P++I+FHGG F   SA+S   DTF
Sbjct: 73  STTNLLTRIYQPASLLHQTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTF 132

Query: 140 CRDFAREIGAIVISVNYRLAPEHRFPSQFDDGFQVLKAIDEGVISETLPENADLSRCFIA 199
           CR      G +V+SV+YR +PEHR+P  +DDG+  L  +   V  ++  ++      ++A
Sbjct: 133 CRRLVTICGVVVVSVDYRRSPEHRYPCAYDDGWNALNWVKSRVWLQSGKDSN--VYVYLA 192

Query: 200 GESAGGNIAHHVMVRAAEYQFKRLKIAGLILIQPFLGGEERRDSELRFGSGYGLTLEMAD 259
           G+S+GGNIAH+V VRA     + +K+ G IL+ P  GG+ER  SE      Y +T++  D
Sbjct: 193 GDSSGGNIAHNVAVRATN---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRD 252

Query: 260 WYWKAWLSIGSNRDHPAANMV---RSNISGLKFPPTLVVIGGLDLLRDRNKEYSNWLEKS 319
           WYW+A+L  G +RDHPA N       ++ G+ FP +LVV+ GLDL++D    Y + L+K+
Sbjct: 253 WYWRAYLPEGEDRDHPACNPFGPRGQSLKGVNFPKSLVVVAGLDLVQDWQLAYVDGLKKT 312

Query: 320 GQEVRVVEYPNGTHGFSWNSDLPEYSMLMQHAKEFTKQI 332
           G EV ++     T GF +  +   +  LM+   +F   I
Sbjct: 313 GLEVNLLYLKQATIGFYFLPNNDHFHCLMEELNKFVHSI 344

[Close]
BLAST of Lsi03G004710 vs. TAIR10
Match: AT3G05120.1 (AT3G05120.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 197.2 bits (500), Expect = 1.6e-50
Identity = 118/315 (37.46%), Postives = 171/315 (54.29%), Query Frame = 1 

Query: 20  RKLANLFTFIL---FRQAF----RSDFTVNRLLLRLLDRKVPPSTSR--GVAAFDTTVDS 79
           R +  L T++L   F+ A+    R D T NR L   LDRKV  + +   GV +FD  +D 
Sbjct: 13  RTVVPLNTWVLISNFKVAYNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDR 72

Query: 80  SSSDLWIRVYNPLTFS--------------DSDPLPIIIYFHGGGFVFGSADSPSIDTFC 139
             + L  RVY P                  D D +P+I++FHGG F   SA+S   DT C
Sbjct: 73  RIN-LLSRVYRPAYADQEQPPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLC 132

Query: 140 RDFAREIGAIVISVNYRLAPEHRFPSQFDDGFQVLKAIDEGVISETLPENADLSRCFIAG 199
           R        +V+SVNYR APE+ +P  +DDG+  L  ++     ++  ++      F+AG
Sbjct: 133 RRLVGLCKCVVVSVNYRRAPENPYPCAYDDGWIALNWVNSRSWLKSKKDSK--VHIFLAG 192

Query: 200 ESAGGNIAHHVMVRAAEYQFKRLKIAGLILIQPFLGGEERRDSELRFGSGYGLTLEMADW 259
           +S+GGNIAH+V +RA E     + + G IL+ P  GG ER +SE      Y +T+   DW
Sbjct: 193 DSSGGNIAHNVALRAGE---SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDW 252

Query: 260 YWKAWLSIGSNRDHPAANMVR---SNISGLKFPPTLVVIGGLDLLRDRNKEYSNWLEKSG 309
           YWKA+L  G +R+HPA N       ++ G+ FP +LVV+ GLDL+RD    Y+  L+K+G
Sbjct: 253 YWKAFLPEGEDREHPACNPFSPRGKSLEGVSFPKSLVVVAGLDLIRDWQLAYAEGLKKAG 312

[Close]
BLAST of Lsi03G004710 vs. TAIR10
Match: AT5G06570.1 (AT5G06570.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 188.3 bits (477), Expect = 7.4e-48
Identity = 100/276 (36.23%), Postives = 159/276 (57.61%), Query Frame = 1 

Query: 48  LLDRKVPPSTSRGVAAFDTTVDSSSSDLWIRVYNPLTFSDSDPLPIIIYFHGGGFVFGSA 107
           L+ +++P   ++ V  F  ++    ++L +R+Y P++ S+   LP++++FHGGGF FGS 
Sbjct: 34  LITQQIPFKNNQTVL-FKDSIYHKPNNLHLRLYKPISASNRTALPVVVFFHGGGFCFGSR 93

Query: 108 DSPSIDTFCRDFAREIGAIVISVNYRLAPEHRFPSQFDDGFQVLKAIDEGVISETLPE-- 167
             P    FC   A  + A+V+S +YRLAPEHR P+ F+D   VL  + +  +S+ +    
Sbjct: 94  SWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFEDAEAVLTWLWDQAVSDGVNHWF 153

Query: 168 ----NADLSRCFIAGESAGGNIAHHVMVR--AAEYQFKRLKIAGLILIQPFLGGEERRDS 227
               + D  R F+ G+S+GGNIAH + VR  +   +   +++ G +L+ PF GGEER +S
Sbjct: 154 EDGTDVDFDRVFVVGDSSGGNIAHQLAVRFGSGSIELTPVRVRGYVLMGPFFGGEERTNS 213

Query: 228 ELRFGSGYGLTLEMADWYWKAWLSIGSNRDHPAAN---MVRSNISGLKFPPTLVVIGGLD 287
           E    S   L+L++ D +W+  L  G+ RDH  AN        +  +   P LV++GG +
Sbjct: 214 E-NGPSEALLSLDLLDKFWRLSLPNGATRDHHMANPFGPTSPTLESISLEPMLVIVGGSE 273

Query: 288 LLRDRNKEYSNWLEK-SGQEVRVVEYPNGTHGFSWN 312
           LLRDR KEY+  L+K  G+ V  +E+ N  HGF  N
Sbjct: 274 LLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGFYSN 307

[Close]
BLAST of Lsi03G004710 vs. NCBI nr
Match: gi|659081174|ref|XP_008441192.1| (PREDICTED: probable carboxylesterase 18 [Cucumis melo])

HSP 1 Score: 553.5 bits (1425), Expect = 2.5e-154
Identity = 269/335 (80.30%), Postives = 295/335 (88.06%), Query Frame = 1 

Query: 1   MSSIGDTQSP-SSPPKLPGKRKLANLFTFILFRQAFRSDFTVNRLLLRLLDRKVPPSTSR 60
           M+S GD QSP SSPP LP  RKLA+LFT  LFR+AFRSDFTVNR LL LLDRKVPP T+R
Sbjct: 1   MASAGDAQSPNSSPPTLPWNRKLADLFTSFLFRRAFRSDFTVNRRLLGLLDRKVPPFTTR 60

Query: 61  GVAAFDTTVDSSSSD-LWIRVYNPLTFSDSDPLPIIIYFHGGGFVFGSADSPSIDTFCRD 120
           GVAA D  +DSSSSD LWIRVYNPLTF +SDPLP+I++FHGGGFV+GSADSP IDTFCRD
Sbjct: 61  GVAASDMNIDSSSSDHLWIRVYNPLTFFNSDPLPVIVFFHGGGFVYGSADSPPIDTFCRD 120

Query: 121 FAREIGAIVISVNYRLAPEHRFPSQFDDGFQVLKAIDEGVISETLPENADLSRCFIAGES 180
           FAREIGAIVISVNYRLAPEHRFPSQFDDGF VLKA++EGVISETLPENADL RCFIAGES
Sbjct: 121 FAREIGAIVISVNYRLAPEHRFPSQFDDGFHVLKAMEEGVISETLPENADLRRCFIAGES 180

Query: 181 AGGNIAHHVMVRAAEYQFKRLKIAGLILIQPFLGGEERRDSELRFGSGYGLTLEMADWYW 240
           AGGNIAHHV VRAAE + KRLKI G+ILIQPFLGGEERRDSE+RFG GYG+T+EMADW+W
Sbjct: 181 AGGNIAHHVTVRAAEGELKRLKIVGMILIQPFLGGEERRDSEIRFGRGYGMTVEMADWFW 240

Query: 241 KAWLSIGSNRDHPAANMVRSNISGLKFPPTLVVIGGLDLLRDRNKEYSNWLEKSGQEVRV 300
           KAWL +GSNRDHPAAN+VRS+ISG+K PP +VVIGGLDLLRDR KEY  WL+K G+EVRV
Sbjct: 241 KAWLPVGSNRDHPAANVVRSSISGVKLPPAVVVIGGLDLLRDRQKEYVEWLKKWGEEVRV 300

Query: 301 VEYPNGTHGFSWNSDLPEYSMLMQHA-KEFTKQIS 333
           VEYPNGTHGF W  DLPEYSMLMQ A K+F    S
Sbjct: 301 VEYPNGTHGFIWKPDLPEYSMLMQDAPKQFANNFS 335

[Close]
BLAST of Lsi03G004710 vs. NCBI nr
Match: gi|449442102|ref|XP_004138821.1| (PREDICTED: probable carboxylesterase 18 [Cucumis sativus])

HSP 1 Score: 550.4 bits (1417), Expect = 2.1e-153
Identity = 266/333 (79.88%), Postives = 292/333 (87.69%), Query Frame = 1 

Query: 1   MSSIGDTQSP-SSPPKLPGKRKLANLFTFILFRQAFRSDFTVNRLLLRLLDRKVPPSTSR 60
           M+S GD QSP SS P LP KRKLA+LFT  LFRQAFRSDFTVNR  LRL DRK+PP TSR
Sbjct: 1   MASTGDAQSPNSSLPTLPWKRKLADLFTSFLFRQAFRSDFTVNRRFLRLFDRKLPPFTSR 60

Query: 61  GVAAFDTTVDSSSSDLWIRVYNPLTFSDSDPLPIIIYFHGGGFVFGSADSPSIDTFCRDF 120
           GVAA D T+DSS+SDLWIRVYNPLTFS+SDPLP+IIYFHGGGFV+GSAD+P  DTFCRDF
Sbjct: 61  GVAASDATIDSSTSDLWIRVYNPLTFSNSDPLPVIIYFHGGGFVYGSADAPPTDTFCRDF 120

Query: 121 AREIGAIVISVNYRLAPEHRFPSQFDDGFQVLKAIDEGVISETLPENADLSRCFIAGESA 180
           AREIGAIVISVNYRLAPE RFPSQFDDGF VLKA+D+G ISET+PENADL RCFIAGESA
Sbjct: 121 AREIGAIVISVNYRLAPEDRFPSQFDDGFHVLKAMDKGAISETVPENADLRRCFIAGESA 180

Query: 181 GGNIAHHVMVRAAEYQFKRLKIAGLILIQPFLGGEERRDSELRFGSGYGLTLEMADWYWK 240
           GGNIAHHV VRAAE +FKR+KI G+ILIQPF GGEERRDSE+RFG GYGLTLEM DW+WK
Sbjct: 181 GGNIAHHVTVRAAESEFKRVKIVGMILIQPFFGGEERRDSEIRFGRGYGLTLEMTDWFWK 240

Query: 241 AWLSIGSNRDHPAANMVRSNISGLKFPPTLVVIGGLDLLRDRNKEYSNWLEKSGQEVRVV 300
           AWL +GSNRDH AAN+V S+ISG+K P  LVVIGGLDLLRDRN+EY  WL+KSGQEVRVV
Sbjct: 241 AWLPVGSNRDHTAANVVGSSISGVKVPAALVVIGGLDLLRDRNREYVEWLKKSGQEVRVV 300

Query: 301 EYPNGTHGFSWNSDLPEYSMLMQHAKEFTKQIS 333
           EYPNGTHGF    DLPEYSML+Q AK+F  +IS
Sbjct: 301 EYPNGTHGFIGKPDLPEYSMLIQDAKQFINKIS 333

[Close]
BLAST of Lsi03G004710 vs. NCBI nr
Match: gi|659131172|ref|XP_008465547.1| (PREDICTED: probable carboxylesterase 18 [Cucumis melo])

HSP 1 Score: 494.6 bits (1272), Expect = 1.4e-136
Identity = 237/300 (79.00%), Postives = 261/300 (87.00%), Query Frame = 1 

Query: 19  KRKLANLFTFILFRQAFRSDFTVNRLLLRLLDRKVPPSTSRGVAAFDTTVDSSSS-DLWI 78
           KRKL+NL + ILFR  FR DFT+NR LLRLLDRK+PPST+RGVAA D  +DSSSS DLWI
Sbjct: 14  KRKLSNLISSILFRLVFRPDFTINRCLLRLLDRKIPPSTTRGVAASDMNIDSSSSHDLWI 73

Query: 79  RVYNPLTFSDSDPLPIIIYFHGGGFVFGSADSPSIDTFCRDFAREIGAIVISVNYRLAPE 138
           RVYNPLTF +SD LP+I+YFHGGGF +GSADS  IDT CRDFAREI AIVISVNYRLAPE
Sbjct: 74  RVYNPLTFFNSDSLPVIVYFHGGGFAYGSADSKPIDTLCRDFAREIRAIVISVNYRLAPE 133

Query: 139 HRFPSQFDDGFQVLKAIDEGVISETLPENADLSRCFIAGESAGGNIAHHVMVRAAEYQFK 198
           HRFPSQFDDGF VLKA+DEGVI ETLPENADL RCFIAGESAGGNIAHHV VRA E + K
Sbjct: 134 HRFPSQFDDGFHVLKAMDEGVIFETLPENADLRRCFIAGESAGGNIAHHVTVRAVECELK 193

Query: 199 RLKIAGLILIQPFLGGEERRDSELRFGSGYGLTLEMADWYWKAWLSIGSNRDHPAANMVR 258
           RLKI G+ILIQPFLGGEERRDSE+RFG GYGLT+EMADW+WKAWL +GSNRDHPAAN+VR
Sbjct: 194 RLKIVGMILIQPFLGGEERRDSEIRFGRGYGLTVEMADWFWKAWLPVGSNRDHPAANVVR 253

Query: 259 SNISGLKFPPTLVVIGGLDLLRDRNKEYSNWLEKSG-QEVRVVEYPNGTHGFSWNSDLPE 317
           S+I G+K PP +VVIGGLDLLRDR KEY  WL K G +EV VV+YP+G HGF WNSDLPE
Sbjct: 254 SSIWGVKLPPAVVVIGGLDLLRDRQKEYVEWLRKLGEEEVLVVDYPSGIHGFCWNSDLPE 313

[Close]
BLAST of Lsi03G004710 vs. NCBI nr
Match: gi|148270935|gb|ABQ53633.1| (esterase [Cucumis melo])

HSP 1 Score: 340.1 bits (871), Expect = 4.3e-90
Identity = 169/324 (52.16%), Postives = 226/324 (69.75%), Query Frame = 1 

Query: 12  SPPKLPGKRKLANLFTFILFRQAFRSDFTVNRLLLRLLDRKVPPSTSR-GVAAFDTTVDS 71
           +P KLP K +L       +  +  R + TVNR L+ L + KV  S+ R GV   DT +D 
Sbjct: 10  NPQKLPLKYRLILPLLSFVSHKCLRPNMTVNRSLISLFESKVSSSSPRDGVFTCDTVIDP 69

Query: 72  SSSDLWIRVYNPLTFSDSDPLPIIIYFHGGGFVFGSADSPSIDTFCRDFAREIGAIVISV 131
           S + LW R++ P +     P+P++IYFHGGGFVF S D  S DT CR  ARE+ AIV+SV
Sbjct: 70  SRN-LWFRLFVPSSTPHDLPIPLLIYFHGGGFVFFSPDFLSFDTLCRKLARELQAIVVSV 129

Query: 132 NYRLAPEHRFPSQFDDGFQVLKAIDEGVISETLPENADLSRCFIAGESAGGNIAHHVMVR 191
           NYRL+PEHR+PSQ++DGF  LK ID+ + S   P+ +D  RCFIAG+SAGGNIAHHV+VR
Sbjct: 130 NYRLSPEHRYPSQYEDGFDALKFIDD-LDSSAFPKKSDFGRCFIAGDSAGGNIAHHVVVR 189

Query: 192 AAEYQFKRLKIAGLILIQPFLGGEERRDSELRFGSGYGLTLEMADWYWKAWLSIGSNRDH 251
           +++Y FK++KI GLI IQPF GGEER +SE+RFG    L LE ADWYWKA+L  G+NR+H
Sbjct: 190 SSDYNFKKVKIRGLIAIQPFFGGEERTESEIRFGRSPTLNLERADWYWKAFLPDGANRNH 249

Query: 252 PAANMVRS---NISGLKFPPTLVVIGGLDLLRDRNKEYSNWLEKSGQEVRVVEYPNGTHG 311
            AA++      NIS +KFP TLV++GG D LRD +++Y  WL+K+G+EV +VEYP   HG
Sbjct: 250 GAAHVFGEDGVNISAVKFPATLVIVGGSDQLRDWDRKYYEWLKKAGKEVELVEYPKAIHG 309

Query: 312 FSWNSDLPEYSMLMQHAKEFTKQI 332
           F   S+LPE  +L++ AK F +++
Sbjct: 310 FYVISELPETWLLIEEAKNFIEKV 331

[Close]
BLAST of Lsi03G004710 vs. NCBI nr
Match: gi|778730917|ref|XP_011659884.1| (PREDICTED: probable carboxylesterase 18 [Cucumis sativus])

HSP 1 Score: 338.6 bits (867), Expect = 1.3e-89
Identity = 169/325 (52.00%), Postives = 225/325 (69.23%), Query Frame = 1 

Query: 12  SPPKLPGKRKLANLFTFILFRQAFRSDFTVNRLLLRLLDRKVPPSTSR--GVAAFDTTVD 71
           +P KLP K +L       +  +A R + TVNR L+ L + KV  S++   GV   DT +D
Sbjct: 10  NPQKLPLKYRLLLRLRSFVSHKALRPNMTVNRSLISLFESKVSSSSTPRDGVFTCDTVID 69

Query: 72  SSSSDLWIRVYNPLTFSDSDPLPIIIYFHGGGFVFGSADSPSIDTFCRDFAREIGAIVIS 131
            S + LW R++ P +     P+P+++YFHGGGFVF S DS   D  CR  ARE+ A+V+S
Sbjct: 70  PSRN-LWFRLFVPSSTPHDLPIPLLVYFHGGGFVFFSPDSLPFDILCRKLARELQAVVVS 129

Query: 132 VNYRLAPEHRFPSQFDDGFQVLKAIDEGVISETLPENADLSRCFIAGESAGGNIAHHVMV 191
           VNYRL+PEHR+PSQ++DGF  LK ID+ + S   PE +D SRCFIAG+SAGGNIAHHV+V
Sbjct: 130 VNYRLSPEHRYPSQYEDGFDALKFIDD-LDSSAFPEKSDFSRCFIAGDSAGGNIAHHVIV 189

Query: 192 RAAEYQFKRLKIAGLILIQPFLGGEERRDSELRFGSGYGLTLEMADWYWKAWLSIGSNRD 251
           R+++Y FK++KI GLI IQPF GGEER +SE+RFG    L LE ADWYWKA+L  G+NR+
Sbjct: 190 RSSDYNFKKVKIRGLIAIQPFFGGEERTESEIRFGETPTLNLERADWYWKAFLPDGANRN 249

Query: 252 HPAANMVRS---NISGLKFPPTLVVIGGLDLLRDRNKEYSNWLEKSGQEVRVVEYPNGTH 311
           H AA++       ISG+KFP TLV++GG D LRD +++Y  WL+K G+EV +VEY N  H
Sbjct: 250 HVAAHVFGEKGVKISGVKFPATLVIVGGSDQLRDWDRKYYEWLKKGGKEVEMVEYANAIH 309

Query: 312 GFSWNSDLPEYSMLMQHAKEFTKQI 332
           GF    +LPE S+L++ AK F  +I
Sbjct: 310 GFYAIPELPETSLLIEEAKNFIGRI 332

The following BLAST results are available for this feature:
BLAST of Lsi03G004710 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CXE18_ARATH4.5e-7144.68Probable carboxylesterase 18 OS=Arabidopsis thaliana GN=CXE18 PE=1 SV=1[more]
GID1C_ARATH1.2e-5541.58Gibberellin receptor GID1C OS=Arabidopsis thaliana GN=GID1C PE=1 SV=1[more]
GID1_ORYSJ7.7e-5538.44Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica GN=GID1 PE=1 SV=1[more]
GID1B_ARATH3.6e-5235.40Gibberellin receptor GID1B OS=Arabidopsis thaliana GN=GID1B PE=1 SV=1[more]
GID1A_ARATH2.8e-4937.46Gibberellin receptor GID1A OS=Arabidopsis thaliana GN=GID1A PE=1 SV=1[more]
BLAST of Lsi03G004710 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LMQ6_CUCSA1.5e-15379.88Uncharacterized protein OS=Cucumis sativus GN=Csa_2G401430 PE=4 SV=1[more]
A5Y726_CUCME3.0e-9052.16Esterase OS=Cucumis melo PE=4 SV=1[more]
A0A0A0LUJ2_CUCSA4.3e-8951.69Uncharacterized protein OS=Cucumis sativus GN=Csa_1G466560 PE=4 SV=1[more]
A0A0A0KXS8_CUCSA2.0e-8651.82Acetyl esterase OS=Cucumis sativus GN=Csa_5G647390 PE=4 SV=1[more]
A0A0A0KUP3_CUCSA1.2e-8349.70Uncharacterized protein OS=Cucumis sativus GN=Csa_5G647370 PE=4 SV=1[more]
BLAST of Lsi03G004710 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G23530.12.5e-7244.68 carboxyesterase 18[more]
AT5G27320.16.7e-5741.58 alpha/beta-Hydrolases superfamily protein[more]
AT3G63010.12.0e-5335.40 alpha/beta-Hydrolases superfamily protein[more]
AT3G05120.11.6e-5037.46 alpha/beta-Hydrolases superfamily protein[more]
AT5G06570.17.4e-4836.23 alpha/beta-Hydrolases superfamily protein[more]
BLAST of Lsi03G004710 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659081174|ref|XP_008441192.1|2.5e-15480.30PREDICTED: probable carboxylesterase 18 [Cucumis melo][more]
gi|449442102|ref|XP_004138821.1|2.1e-15379.88PREDICTED: probable carboxylesterase 18 [Cucumis sativus][more]
gi|659131172|ref|XP_008465547.1|1.4e-13679.00PREDICTED: probable carboxylesterase 18 [Cucumis melo][more]
gi|148270935|gb|ABQ53633.1|4.3e-9052.16esterase [Cucumis melo][more]
gi|778730917|ref|XP_011659884.1|1.3e-8952.00PREDICTED: probable carboxylesterase 18 [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0016787hydrolase activity
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR013094AB_hydrolase_3
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016787 hydrolase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G004710.1Lsi03G004710.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 94..308
score: 6.3
NoneNo IPR availablePANTHERPTHR23024MEMBER OF 'GDXG' FAMILY OF LIPOLYTIC ENZYMEScoord: 36..327
score: 5.5E
NoneNo IPR availablePANTHERPTHR23024:SF174SUBFAMILY NOT NAMEDcoord: 36..327
score: 5.5E