Lsi03G004650 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi03G004650
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionMagnesium transporter NIPA2-like protein
Locationchr03 : 5247770 .. 5254413 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAGGCGTTTTGTAAATGAGAAAGATAAACATAACATAGCAGTTCGTCACTTCCATGAGAGCAAGAGAGAGAAATCTCATCCTTTTGTAACCAACGCACAGTTTGTATGTCACCTTCTCCTCGCTTTCTGATCTAACACGCACCATCGAAATCTCGTTTGATCTCTCTTGATCAATTCATCTCCTTCATCTCCATCACTGAACAAATTTCTTCATCCTTTCCGCGCGATCCAACCCCTCCCTCTGTTTTTCCCAGATTTTCCCTCGTCTTTTTTGGTGCACCACCGGGTATTTTCTCTCCGATGACACCGCTTTCAACCGCCTTCATTCTCATTACCATGGCGGATTTGATGGGGCTACATTGAGTTTGCATGGATCGTATGGAATGGAAGCTTTTCGATTTATGAGTTCTTATTTTCAACTCCTCCTCCAATTTCAGCCATGGCGGTTAGTGGGCCGGCTTCGAGTTCAGAGAGAGCCTGGAGTGACTCTCGCAAGGGTATGTCCAGTGATAACATCAAGGGTTTGGTCTTAGCTTTGTCTTCCAGTTTCTTCATTGGTGCTAGCTTCATTGTCAAGAAGAAAGGCCTTAAGATAGCCGGGGCTTCTGGTGTTAGGGCAGGTAATGCACTTTTTATTATCTCTTCTCTTTTCAAACGAATGTTTGATTTTTGGATCCATGTTGGTTGCCTTTCCCTACTCTTAATCTCAATCTTTCCCTGGCAGGCGCTGGCGGTTATTCTTACTTGTATGAACCATTATGGTGGGTCGGCATGATAACCAGTAAGCTTGTCCAATCTATGTGGTTGCTGCTGTGTTTTAGTTACGGGCTTGGTTGATACGAAATTATTTTGAGAATTATAAGCCCCCTAGAAGAAGTGACTATTGAAGTTATTCTATCTGTGTAGCATTATAAACTAGTAAATGATGAATTATACTGAAGTATTTCTGCCGTTCTGGTCCTCGGTGGCTAACACATTGATAAGTGGATGCATTTAGGCCCTGGCTTTTGATATCAGTGTCTTGGGAATATATTATCCATGTGTTTGTGTGTGTCTTTGGACTAAGATAAACTCTGTAAGTTGGTTCCATTTTGTGGCTAGTTATCATTGGCTTCTGATGCTTTTTCATTCATTCTTCTGTTCCAAGTTAGACATTGTCCCCTTCTCCCAGTTTCTTTGCTTTTTCAGTTACTTCTATATTTGGCCCTCAATTTCTTCGTAGGTGGATTCTTATTTACAAGTTAGGAGCAAGATAATATTGCAGAGAATTATTTGTTGCATTATGATCTCCTTTTCCTTTTGCATTCCTTTTTGAAGTATTAAGTTGAGATGTACTTACATAACTAAAACCTTGAGTATTAAATATTTCATCAGAAGATTTGTATTCTAATGGAAATATTTTTCACTTGGCAGTGGTTATAGGGGAAATTGCTAATTTTGCAGCTTATGCATTTGCACCAGCTATTCTTGTAACTCCCCTCGGTGCACTCAGTATAATTATCAGGCAAGAGGACAAACTGTAGCTGTTGTTGTTGTTAACGCATTTTGGCATTCACCGTGTTTTGAGCATTTTGTGACATGTTATGTGCAGTGCTGCTCTTGCTCATATTATATTAAGGGAGCGATTAAACATTTTTGGTATTCTTGGTTGTGTGTTATGTGTTGTGGGTTCGACAACAATTGTTCTGCATGCACCGCAAGAGCGTGAGATAGAATCTGTTAAGGAAGTGTGGGATCTTGCAACAGAACCAGGTATACACTAAAGGACATTCTATTTCTATTACCATGTTTAATTTTTAACTTTTGTGTGAATATCAAATACATTCACGGCTTCTCTCCTTAATGTAACATGTGCATGTTTTCTGTAGCTTTCCTTTTGTATGCTGCTTTGATGATAGCCACAACTCTCGTACTCATAATCCACTTTGTGCCTCATTATGGGCAGACATATGTGATGGTCTACATAGGGGTTTGTTCTATTGTGGGTTCACTTTCGGTATGTAGTCGGTTTTTCATGCGTTTTTTTTCTTTTAGTTTTTACACCGCTCTATGTAAAGTTCTGAAGCCGTTTTGACAGGTTATGTGTGTTAAAGCACTTGGCATTGCCCTGAAGTTAACGTTTTCTGGAATGAATCAGTTAACATATCCTCAAACTTGGGCCTTCACAATGTTTGTCATTACTTGTGTGATCATTCAAATAAATTATCTAAACAAGGTATCCATCTTTATATTTGAAAGCATTAAATTAATTATTTAACCACTTTTGACTTTTGAGGGATCATCAACTAGTGGACGAGTTAATTGTTTGGTAATTGGCACAATATAGTTGGTTAGTTGTATACATACGTTGGTAATTGGTAGTGTTCCAGCATGCTGCAAGGGTTGAAGTTGGCACATTCTTGGCAAAGTCCTCTTGTGCTATTTCATAATGAATTCCTGAATCTTTTGTTATGGTCAATTGTCAGGTTGTGGGAAATAGTCACAATTATTAAGTGACATCATGTTCACTTTTGAGGAAAAAAAAGAAAAAAGAAAAAAAGAAAAGTGACATCACGTTCTAAAATCCCAAAGTTCCTACGTTGAAGCAATTGAAAGTTTCATTAGGTTAATAGTCACATATGCTTGATAAAGATTTACAAACTAATAAATTTAAGCAATGTGCAATGCAGACTTCTAAATATACAACTTTAATTATCAGAACGATTATGACATTATCATAATTTCTCTATTCGGACATGTGAACTGAGAATACAAACTACAGGCTATAACCTGGAAGAGATGAAAATTTTCCTTAATTTTGATGACCGGCCTGATTGTGTTTTTAAATAAGAATTTGAAATTTTCTGATGGTATTTTATTATTGTAGGCTCTTGATACTTTCAACACTGCTGTAGTATCCCCTACATACTATGTGATGTTTACATCCTTGACCATCTTGGCTAGCATAATTATGTTCAAGGTAAAGACATTTAGCTCTTATTTTCCCTGGGCTTCATAAAACATTATTGATTTCTTCTGTCTGATAAATATAAGAGGCAATGAACGCATTATGTTCAAGATTTTCACTTGTGAATTTAACAAATATTCCTCTTCTTTTATGCCTGCATTTCTGTTTGTGTGTTGTACATTTTTAGGGAAATGAAAGTTGCTTTCAATGAGCAGTCTTTTGTTCAAATCTTCAAGTATAGAGTCAACTGTTGTATGTTGCTGTTGCCTGATTTCAAATATAGAGTGCGCGGGTAAGGTAATTGCTTGCAGTTGGTCTTTGAGAACATTGGACAGTAGCCATCGGTATCAAGATTCAATGAATTAAGTTCAAGGCCATTAATTCATGTGGATATGTGCGATCTGAAATAAATCCAGAAGTATATAATCCTGTAAAAACATTTTCTCCTGATTCCCAATGCCTAATGCTTGTGGAGAAGACAAGCTTTTGCATAACTTCAAAAGATTCTGGATTTCAAGTAAAAACAGTTTTCGTTTTTTTCTTCAAGCTTGTGATAACTATATTGAATAGATAAGCTTCTACTGCCACAGTTACAGAGAAACTCCAGGTCCCTAAATTTCCTGAATAGCTTATAATCCAAGTAAATCCCCGCTAAACCTTTCCTGCTTTTTCGTTCACCCTCATCTTAACTAACCAGTTCCTTGCCCACTACTAATGCCTTTGTTCATGCAAAGATGCACCAACCTATGCTTCCTCTTCTATCAAGGCAGCCAGTTCCCGAGATACTTTAATTCTCTCACTTTACTAACTTTTTACTCTTTTAACGTAGGTATTATTTTGCACCAGGGCTCTATCAGCATTCCCCTTCCAAAGTTTCAAACTGTGAAAAAACTTTTCATATCCATAGCTGTTTATCATTTCTATCAATAATTTGAGCTCTGGGGTCAATTTAGATAGGAGGGAGGGCCTCTCTCCTTGAATCTTAATAGAATAACTAAAAGCCAGTCTCAACCGAAGCACAGGATCAATGCACCCGGTAGAGGTTAAAGGGAAATGATAAAAATTTGAAGCAAGCTTCTTAGGGAGGCATTGTGGCAGTAACTTCTGTCTATTATATTTTGTTCGAGGAATACCTGATTCTCACCTTAAAAGACGATGCCTTTCTTCTCATTTCAGTCTAATGTTTCATTTGATGTGCTTCCATAATAAATTTCATCAGCACATCTCTTTTTTGTAATGTGCTCGACAGCATCCAAAGGAAAATCACTGTTTTGATGGACATGGGCTGATGGATCCTTACCTTATATTGCCTTAAAAGGGTGTAGTTGAGTTTTATTGATAAATGAATAGAGACTAGAGACTAATGCTTAGAAGATACAAACTCTACTTGGGACAGAAAATATTAATAATAAATTACTAATGTAAAAACATCCCAATTTAAACAAAAATCCTGATAAAAGCACCAGCAAGGCTTGGGAAGAGAACATTAAGGAGAGGCCTTTAATCCAGCTGACACAAAACAATCTAGTTAAAGAAGAAGTTTGCAAGCATAGATAATTAGATTCCTTTCCAAGCAAGACATCACAGAACTCCCGATACTGCTAAAATCCACTCAACAAAACCTCCAAAAAAAAACTTGTTGCCAAACACTTAATTTAAAGCGCGAGATAATCCACACTCCTCCAAGCTATAGAAATGCTGAGCCAGCAAAAGGGAGTAGTGAGTTGGAGAATGGAAAGATGTTTTGTATGAAAAACCCGCCAGTAGCATAGCAACAACCATATGTAATATCCAAGTTCTGTCAAACACAAAGCAATGCAAATAGGTTCCAAGAAAATGACCACATCCGACAGCATCATGCTGTCGATGGGAAAAGGTACTTCTCTTGAGCTTTATCCGTTGTAGTTGAAACGTGAAAGAACTCAAGGCAGGAAGTGCCAATCCAATATTGGAATGAACTAGTCTGCAGCCGCTCCCCATTTTAACACAACGTTGCCTAGAAATTCCAACTGTTCTCTAGACTCTAGCAAAAGACACCCCATGAAGTTGGCATATAATTGGAGCTGCTAAAGAACAGTGGATATGGCATTCGGATGCCCAATGTGGTCATTCCAATTTCGAGTGTAAAACAAGAATGTCTTCAAAGCACCAACCTTAGGTATGCTTGAATACTTTGTTCATCATTGATTGGAAAGTGGCTGTGGGGTGTTCACGAGGCTGAACATATAACAACAACAATTAACATATTTGATATCTATCATGCATGCTGTACTGACAGCCTGTCTCTTATGTACTCCCTACTCTTTAACAAGAATTTGGTGAAGAGTAAGACCTAACTTTAGGGGTCTAGCTTAGATGATATGGTTGCTCCATGTAGCTCATCTTTAACCAGGACTGGAAACTTGACCATCAGACTGACCATTTCCTTATCAAGAGCATGGTAGTCTGCAAAATCGTATCATTTTTCCTTCTAACAAATAATACAGGGCTGAAAACGCGCTTACATTGGTTGGATGATACCCATATCAATAAGGAGAGACGATGGAAAATTCACATTTCTTCCTGTAGTCTACTACTGCCTACTTTATCATTTTCTAGAACAAATCAATTGAATGTCGTGGTTTAGTCTTCAATTGTCAACTGTATTCTTAACTATTCTGATCAATTGATAGAATTCACTACGAACGCTCATGAATTTCTAATCTAAATGCGCTCTTGTTCATGCAAAGCACCAAACTGCCCTTCCTTTTTCATTCTCATTATTTTATTACCCTTTCCTTGTTGCCTTTTCCTATTTTAATCATGTATGTATGATGCACTGGGGTCTATTATTTTGTCGTTATTATACTTTCGAAAGGTGATGAACTACATTTATTTCCCTTTTAATTATATGTTTAGTCTTACTTTTTGGGACTCATCGGGATGAATTTTCATTATTGATGCTGATTAACCTGTTCTTTAACTTCTTATCAGGATTGGGATCGACAGAGTGCAATCCAAATTTTCACACAAATGTGTGGTTTTGTGACGATTCTCGCTGGTACTTTTCTTCTTCACAGAACGAAGGATATGGTTGAGGGTGGGTCTTCAGTTCCTCCTTCCATTGTTTTAATCCATATCTGTTTCAAAATATCTAATAATCGAAATTTCGATTCATTGTAGCATCGACAACACCATCTTTTTCAATGCGACTTTCTAAACACATAGAAGATGGTTGTGAACTTGAAGCCATCCCACTTCAGCGTCAAGCATCATTGTGATAAGATTAGACATTAGTCGTTAGATCTGCGAATTCACTCTTATCATCATATCGCATTTCGTGCATAAATCTTAGGGGAAGCTATGGCCGCCATTGGCTTTGTGATCGTGAACATTGTTGGGTAAATTTGAAGGGGATACAAGCATGGCTATGGAAGGACGGTAATCTCTTTTCTTCCTTGGAACTCCCATTTTTTTTCTGTTAGGTACTCCTTTGTGAGTTGCAGTTTTTGGTCCAGTGTTCTATATACCTATATTATAACATTCATTCATTTCCACCAAGAAATAGACTCTTTAAGGGATGTTTGGCTCATAGAGTAGCTTGGTAGAGCAAGCTGGAAGGACCATTTACATACTTGATTAAACTTCACTCATGTACTCCCTTTTTCTTCACTTCTTTCTTGATTTAATAGAAATTTCATTCTAGAATTGCCCAC

mRNA sequence

CAAAGGCGTTTTGTAAATGAGAAAGATAAACATAACATAGCAGTTCGTCACTTCCATGAGAGCAAGAGAGAGAAATCTCATCCTTTTGTAACCAACGCACAGTTTGTATGTCACCTTCTCCTCGCTTTCTGATCTAACACGCACCATCGAAATCTCGTTTGATCTCTCTTGATCAATTCATCTCCTTCATCTCCATCACTGAACAAATTTCTTCATCCTTTCCGCGCGATCCAACCCCTCCCTCTGTTTTTCCCAGATTTTCCCTCGTCTTTTTTGGTGCACCACCGGGTATTTTCTCTCCGATGACACCGCTTTCAACCGCCTTCATTCTCATTACCATGGCGGATTTGATGGGGCTACATTGAGTTTGCATGGATCGTATGGAATGGAAGCTTTTCGATTTATGAGTTCTTATTTTCAACTCCTCCTCCAATTTCAGCCATGGCGGTTAGTGGGCCGGCTTCGAGTTCAGAGAGAGCCTGGAGTGACTCTCGCAAGGGTATGTCCAGTGATAACATCAAGGGTTTGGTCTTAGCTTTGTCTTCCAGTTTCTTCATTGGTGCTAGCTTCATTGTCAAGAAGAAAGGCCTTAAGATAGCCGGGGCTTCTGGTGTTAGGGCAGGCGCTGGCGGTTATTCTTACTTGTATGAACCATTATGGTGGGTCGGCATGATAACCATGGTTATAGGGGAAATTGCTAATTTTGCAGCTTATGCATTTGCACCAGCTATTCTTGTAACTCCCCTCGGTGCACTCAGTATAATTATCAGGCAAGAGGACAAACTTGCTGCTCTTGCTCATATTATATTAAGGGAGCGATTAAACATTTTTGGTATTCTTGGTTGTGTGTTATGTGTTGTGGGTTCGACAACAATTGTTCTGCATGCACCGCAAGAGCGTGAGATAGAATCTGTTAAGGAAGTGTGGGATCTTGCAACAGAACCAGCTTTCCTTTTGTATGCTGCTTTGATGATAGCCACAACTCTCGTACTCATAATCCACTTTGTGCCTCATTATGGGCAGACATATGTGATGGTCTACATAGGGGTTTGTTCTATTGTGGGTTCACTTTCGGTTATGTGTGTTAAAGCACTTGGCATTGCCCTGAAGTTAACGTTTTCTGGAATGAATCAGTTAACATATCCTCAAACTTGGGCCTTCACAATGTTTGTCATTACTTGTGTGATCATTCAAATAAATTATCTAAACAAGGCTCTTGATACTTTCAACACTGCTGTAGTATCCCCTACATACTATGTGATGTTTACATCCTTGACCATCTTGGCTAGCATAATTATGTTCAAGGATTGGGATCGACAGAGTGCAATCCAAATTTTCACACAAATGTGTGGTTTTGTGACGATTCTCGCTGGTACTTTTCTTCTTCACAGAACGAAGGATATGGTTGAGGCATCGACAACACCATCTTTTTCAATGCGACTTTCTAAACACATAGAAGATGGTTGTGAACTTGAAGCCATCCCACTTCAGCGTCAAGCATCATTGTGATAAGATTAGACATTAGTCGTTAGATCTGCGAATTCACTCTTATCATCATATCGCATTTCGTGCATAAATCTTAGGGGAAGCTATGGCCGCCATTGGCTTTGTGATCGTGAACATTGTTGGGTAAATTTGAAGGGGATACAAGCATGGCTATGGAAGGACGGTAATCTCTTTTCTTCCTTGGAACTCCCATTTTTTTTCTGTTAGGTACTCCTTTGTGAGTTGCAGTTTTTGGTCCAGTGTTCTATATACCTATATTATAACATTCATTCATTTCCACCAAGAAATAGACTCTTTAAGGGATGTTTGGCTCATAGAGTAGCTTGGTAGAGCAAGCTGGAAGGACCATTTACATACTTGATTAAACTTCACTCATGTACTCCCTTTTTCTTCACTTCTTTCTTGATTTAATAGAAATTTCATTCTAGAATTGCCCAC

Coding sequence (CDS)

ATGGCGGTTAGTGGGCCGGCTTCGAGTTCAGAGAGAGCCTGGAGTGACTCTCGCAAGGGTATGTCCAGTGATAACATCAAGGGTTTGGTCTTAGCTTTGTCTTCCAGTTTCTTCATTGGTGCTAGCTTCATTGTCAAGAAGAAAGGCCTTAAGATAGCCGGGGCTTCTGGTGTTAGGGCAGGCGCTGGCGGTTATTCTTACTTGTATGAACCATTATGGTGGGTCGGCATGATAACCATGGTTATAGGGGAAATTGCTAATTTTGCAGCTTATGCATTTGCACCAGCTATTCTTGTAACTCCCCTCGGTGCACTCAGTATAATTATCAGGCAAGAGGACAAACTTGCTGCTCTTGCTCATATTATATTAAGGGAGCGATTAAACATTTTTGGTATTCTTGGTTGTGTGTTATGTGTTGTGGGTTCGACAACAATTGTTCTGCATGCACCGCAAGAGCGTGAGATAGAATCTGTTAAGGAAGTGTGGGATCTTGCAACAGAACCAGCTTTCCTTTTGTATGCTGCTTTGATGATAGCCACAACTCTCGTACTCATAATCCACTTTGTGCCTCATTATGGGCAGACATATGTGATGGTCTACATAGGGGTTTGTTCTATTGTGGGTTCACTTTCGGTTATGTGTGTTAAAGCACTTGGCATTGCCCTGAAGTTAACGTTTTCTGGAATGAATCAGTTAACATATCCTCAAACTTGGGCCTTCACAATGTTTGTCATTACTTGTGTGATCATTCAAATAAATTATCTAAACAAGGCTCTTGATACTTTCAACACTGCTGTAGTATCCCCTACATACTATGTGATGTTTACATCCTTGACCATCTTGGCTAGCATAATTATGTTCAAGGATTGGGATCGACAGAGTGCAATCCAAATTTTCACACAAATGTGTGGTTTTGTGACGATTCTCGCTGGTACTTTTCTTCTTCACAGAACGAAGGATATGGTTGAGGCATCGACAACACCATCTTTTTCAATGCGACTTTCTAAACACATAGAAGATGGTTGTGAACTTGAAGCCATCCCACTTCAGCGTCAAGCATCATTGTGA

Protein sequence

MAVSGPASSSERAWSDSRKGMSSDNIKGLVLALSSSFFIGASFIVKKKGLKIAGASGVRAGAGGYSYLYEPLWWVGMITMVIGEIANFAAYAFAPAILVTPLGALSIIIRQEDKLAALAHIILRERLNIFGILGCVLCVVGSTTIVLHAPQEREIESVKEVWDLATEPAFLLYAALMIATTLVLIIHFVPHYGQTYVMVYIGVCSIVGSLSVMCVKALGIALKLTFSGMNQLTYPQTWAFTMFVITCVIIQINYLNKALDTFNTAVVSPTYYVMFTSLTILASIIMFKDWDRQSAIQIFTQMCGFVTILAGTFLLHRTKDMVEASTTPSFSMRLSKHIEDGCELEAIPLQRQASL
BLAST of Lsi03G004650 vs. Swiss-Prot
Match: NIPA4_ARATH (Probable magnesium transporter NIPA4 OS=Arabidopsis thaliana GN=At1g71900 PE=2 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 2.8e-140
Identity = 265/348 (76.15%), Postives = 297/348 (85.34%), Query Frame = 1

Query: 8   SSSERAWSDSRKGMSSDNIKGLVLALSSSFFIGASFIVKKKGLKIAGASGVRAGAGGYSY 67
           + S  +W DS KGMSSDNIKGLVLALSSS FIGASFIVKKKGLK A ++G RAG GGYSY
Sbjct: 2   AESSGSWRDSYKGMSSDNIKGLVLALSSSLFIGASFIVKKKGLKKAASTGTRAGVGGYSY 61

Query: 68  LYEPLWWVGMITMVIGEIANFAAYAFAPAILVTPLGALSIIIRQEDKLAALAHIILRERL 127
           LYEPLWW+GM TM++GEIANFAAYAFAPAILVTPLGA+SIII      A LAHIILRE+L
Sbjct: 62  LYEPLWWIGMTTMLLGEIANFAAYAFAPAILVTPLGAVSIIIS-----AVLAHIILREKL 121

Query: 128 NIFGILGCVLCVVGSTTIVLHAPQEREIESVKEVWDLATEPAFLLYAALMIATTLVLIIH 187
           +IFGILGC LCVVGSTTIVLHAPQEREI+SV EVW+LATEPAF+ YA+L+I   + LII 
Sbjct: 122 HIFGILGCALCVVGSTTIVLHAPQEREIDSVIEVWNLATEPAFMFYASLVIGAAVFLIIR 181

Query: 188 FVPHYGQTYVMVYIGVCSIVGSLSVMCVKALGIALKLTFSGMNQLTYPQTWAFTMFVITC 247
           FVP YGQT VMVYIG+CS+VGSLSVM VKALGIALKLTFSG NQL YPQTW FT+ V+TC
Sbjct: 182 FVPQYGQTNVMVYIGICSLVGSLSVMSVKALGIALKLTFSGTNQLFYPQTWIFTLVVLTC 241

Query: 248 VIIQINYLNKALDTFNTAVVSPTYYVMFTSLTILASIIMFKDWDRQSAIQIFTQMCGFVT 307
           V+ Q+NYLNKALDTFNTA+VSP YYVMFTSLTILAS+IMFKDWDRQ+  QI T++CGFVT
Sbjct: 242 VVTQLNYLNKALDTFNTAIVSPIYYVMFTSLTILASVIMFKDWDRQNGTQIVTEICGFVT 301

Query: 308 ILAGTFLLHRTKDMVEASTTPSFSMRLSKHIEDGCELEAIPLQRQASL 356
           IL+GTFLLHRTKDMVE S+     +R+SKHI    E E IPL+RQ SL
Sbjct: 302 ILSGTFLLHRTKDMVEGSSV-ILPLRISKHIN---EEEGIPLRRQESL 340

BLAST of Lsi03G004650 vs. Swiss-Prot
Match: NIPA3_ARATH (Probable magnesium transporter NIPA3 OS=Arabidopsis thaliana GN=At1g34470 PE=2 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 3.7e-140
Identity = 262/345 (75.94%), Postives = 300/345 (86.96%), Query Frame = 1

Query: 8   SSSERAWSDSRKGMSSDNIKGLVLALSSSFFIGASFIVKKKGLKIAGASGVRAGAGGYSY 67
           +S   +W D+ KGMSSDNIKGLVLALSSS FIGASFIVKKKGLK AGASG+RAG+GGYSY
Sbjct: 2   ASLSGSWRDAYKGMSSDNIKGLVLALSSSLFIGASFIVKKKGLKRAGASGLRAGSGGYSY 61

Query: 68  LYEPLWWVGMITMVIGEIANFAAYAFAPAILVTPLGALSIIIRQEDKLAALAHIILRERL 127
           L EPLWWVGMITM++GEIANFAAYAFAPAILVTPLGALSIII      AALAH+IL E+L
Sbjct: 62  LLEPLWWVGMITMIVGEIANFAAYAFAPAILVTPLGALSIIIS-----AALAHVILHEKL 121

Query: 128 NIFGILGCVLCVVGSTTIVLHAPQEREIESVKEVWDLATEPAFLLYAALMIATTLVLIIH 187
           + FG+LGCVLCVVGS TIVLHAPQE+EI+SV +VW+LATEPAFLLYAA ++   ++LI+ 
Sbjct: 122 HTFGLLGCVLCVVGSITIVLHAPQEQEIDSVLQVWNLATEPAFLLYAAAVVGAAIILIVQ 181

Query: 188 FVPHYGQTYVMVYIGVCSIVGSLSVMCVKALGIALKLTFSGMNQLTYPQTWAFTMFVITC 247
           FVP YGQ++VMVYIGVCS+VGSLSVM VKALGIALKLTFSGMNQL YPQTW FT+ V+TC
Sbjct: 182 FVPQYGQSHVMVYIGVCSLVGSLSVMSVKALGIALKLTFSGMNQLIYPQTWVFTLIVLTC 241

Query: 248 VIIQINYLNKALDTFNTAVVSPTYYVMFTSLTILASIIMFKDWDRQSAIQIFTQMCGFVT 307
           VI Q+NYLNKALDTFNTAVVSP YYVMFTSLTILAS+IMFKDWDRQ   QI T++CGFVT
Sbjct: 242 VITQMNYLNKALDTFNTAVVSPIYYVMFTSLTILASVIMFKDWDRQDGTQIVTELCGFVT 301

Query: 308 ILAGTFLLHRTKDMVEASTT-PSFSMRLSKHIED--GCELEAIPL 350
           IL+GTFLLH+TKDMV+ S++  + ++RL K +ED  G E E IPL
Sbjct: 302 ILSGTFLLHKTKDMVDGSSSLGNLALRLPKQLEDSNGFEQEGIPL 341

BLAST of Lsi03G004650 vs. Swiss-Prot
Match: NIPA5_ARATH (Probable magnesium transporter NIPA5 OS=Arabidopsis thaliana GN=At4g09640 PE=2 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 8.9e-134
Identity = 249/339 (73.45%), Postives = 286/339 (84.37%), Query Frame = 1

Query: 10  SERAWSDSRKGMSSDNIKGLVLALSSSFFIGASFIVKKKGLKIAGASGVRAGAGGYSYLY 69
           S  +W D+ KGMSSDN+KGLVLALSSS FIGASFIVKKKGLK AGASG+RAG+GGYSYL 
Sbjct: 4   SSGSWRDAYKGMSSDNVKGLVLALSSSIFIGASFIVKKKGLKKAGASGLRAGSGGYSYLL 63

Query: 70  EPLWWVGMITMVIGEIANFAAYAFAPAILVTPLGALSIIIRQEDKLAALAHIILRERLNI 129
           EPLWW+GMITM++GEIANFAAYAFAPAILVTPLGALSIII      A+LAHIIL+E+L+ 
Sbjct: 64  EPLWWIGMITMIVGEIANFAAYAFAPAILVTPLGALSIIIS-----ASLAHIILQEKLHT 123

Query: 130 FGILGCVLCVVGSTTIVLHAPQEREIESVKEVWDLATEPAFLLYAALMIATTLVLIIHFV 189
           FGILGC LC+VGS TIVLHAPQE++I SV EVW+LATEPAFL YAA ++   +VLI+ F+
Sbjct: 124 FGILGCALCIVGSVTIVLHAPQEQDIVSVLEVWNLATEPAFLFYAAAVVGAAIVLIVQFI 183

Query: 190 PHYGQTYVMVYIGVCSIVGSLSVMCVKALGIALKLTFSGMNQLTYPQTWAFTMFVITCVI 249
           P YGQ++VMVYIGVCS++GSLSVM VKALGIALKLTFSG NQL YPQTW FT+ V+ CVI
Sbjct: 184 PLYGQSHVMVYIGVCSLIGSLSVMSVKALGIALKLTFSGTNQLGYPQTWVFTVIVLFCVI 243

Query: 250 IQINYLNKALDTFNTAVVSPTYYVMFTSLTILASIIMFKDWDRQSAIQIFTQMCGFVTIL 309
            Q+NYLNKALDTFNTAVVSP YYVMFTSLTILAS+IMFKDWDRQS  QI T++CGFVTIL
Sbjct: 244 TQMNYLNKALDTFNTAVVSPIYYVMFTSLTILASVIMFKDWDRQSGTQIMTELCGFVTIL 303

Query: 310 AGTFLLHRTKDMVEASTTPSFS--------MRLSKHIED 341
           +GTFLLH T DMV+  +  + S        +R+ KH ED
Sbjct: 304 SGTFLLHTTTDMVDGESKGNLSSEEDSHLLLRIPKHSED 337

BLAST of Lsi03G004650 vs. Swiss-Prot
Match: NIPA1_ARATH (Probable magnesium transporter NIPA1 OS=Arabidopsis thaliana GN=At3g23870 PE=2 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 3.2e-115
Identity = 211/313 (67.41%), Postives = 259/313 (82.75%), Query Frame = 1

Query: 21  MSSDNIKGLVLALSSSFFIGASFIVKKKGLKIAGASGVRAGAGGYSYLYEPLWWVGMITM 80
           MS DNI G++LA+SSS FIG+SFI+KKKGLK AGASGVRAG GGY YL EP WW GMITM
Sbjct: 4   MSPDNINGVILAVSSSIFIGSSFIIKKKGLKKAGASGVRAGEGGYGYLKEPWWWAGMITM 63

Query: 81  VIGEIANFAAYAFAPAILVTPLGALSIIIRQEDKLAALAHIILRERLNIFGILGCVLCVV 140
           ++GE+ANFAAYAFAPAILVTPLGALSII       A LAH IL+E+L++FGILGC+LCVV
Sbjct: 64  IVGEVANFAAYAFAPAILVTPLGALSIIFS-----AVLAHFILKEKLHMFGILGCILCVV 123

Query: 141 GSTTIVLHAPQEREIESVKEVWDLATEPAFLLYAALMIATTLVLIIHFVPHYGQTYVMVY 200
           GSTTIVLHAP E++IESVK++W LA EP FL+Y+A+++    +LI ++ P YG+T+++VY
Sbjct: 124 GSTTIVLHAPHEQKIESVKQIWQLAIEPGFLVYSAVIVIVVAILIFYYEPRYGKTHMIVY 183

Query: 201 IGVCSIVGSLSVMCVKALGIALKLTFSGMNQLTYPQTWAFTMFVITCVIIQINYLNKALD 260
           +G+CS++GSL+VM VKA+ IA+KLTFSG NQ  Y  TW F + V TC I+QINYLNKALD
Sbjct: 184 VGICSLMGSLTVMSVKAVAIAIKLTFSGTNQFKYFNTWIFILVVATCCILQINYLNKALD 243

Query: 261 TFNTAVVSPTYYVMFTSLTILASIIMFKDWDRQSAIQIFTQMCGFVTILAGTFLLHRTKD 320
           TFNTAV+SP YYVMFT+ TI+AS+IMFKDW  QS ++I T++CGFVTIL+GTFLLH+TKD
Sbjct: 244 TFNTAVISPVYYVMFTTFTIIASMIMFKDWASQSGLKIATELCGFVTILSGTFLLHKTKD 303

Query: 321 M-VEASTTPSFSM 333
           M   AS   S SM
Sbjct: 304 MGNSASGRGSISM 311

BLAST of Lsi03G004650 vs. Swiss-Prot
Match: NIPA2_ARATH (Probable magnesium transporter NIPA2 OS=Arabidopsis thaliana GN=At4g13800 PE=2 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 7.0e-115
Identity = 210/301 (69.77%), Postives = 249/301 (82.72%), Query Frame = 1

Query: 21  MSSDNIKGLVLALSSSFFIGASFIVKKKGLKIAGASGVRAGAGGYSYLYEPLWWVGMITM 80
           MS DNI G++LA+SSS FIG+SFI+KKKGLK AG SG RAG GGY YLYEP WW GMITM
Sbjct: 4   MSPDNIHGVILAVSSSIFIGSSFIIKKKGLKKAGVSGARAGEGGYGYLYEPWWWAGMITM 63

Query: 81  VIGEIANFAAYAFAPAILVTPLGALSIIIRQEDKLAALAHIILRERLNIFGILGCVLCVV 140
           ++GEIANFAAYAFAPAILVTPLGALSII       A LAH IL E+L++FGILGCVLCVV
Sbjct: 64  IVGEIANFAAYAFAPAILVTPLGALSIIFS-----AVLAHFILEEKLHMFGILGCVLCVV 123

Query: 141 GSTTIVLHAPQEREIESVKEVWDLATEPAFLLYAALMIATTLVLIIHFVPHYGQTYVMVY 200
           GSTTIVLHAP E+ IESVK+VW LATEP FL Y+A+++   L LI ++ P YG+T+++VY
Sbjct: 124 GSTTIVLHAPHEQGIESVKQVWHLATEPGFLAYSAVVLVVVLALIFYYEPRYGKTHMIVY 183

Query: 201 IGVCSIVGSLSVMCVKALGIALKLTFSGMNQLTYPQTWAFTMFVITCVIIQINYLNKALD 260
           +G+CS++GSL+VM VKA+ IA+KLTFSGMNQ  Y   W F + V  C I+QINYLNKALD
Sbjct: 184 VGICSLMGSLTVMSVKAVAIAIKLTFSGMNQFKYFHAWIFIIVVTICCILQINYLNKALD 243

Query: 261 TFNTAVVSPTYYVMFTSLTILASIIMFKDWDRQSAIQIFTQMCGFVTILAGTFLLHRTKD 320
            FNTAV+SP YYVMFT+ TILAS+IMFKDW  QS +QI T++CGFVTIL+GTFLLH+TKD
Sbjct: 244 NFNTAVISPVYYVMFTTFTILASMIMFKDWASQSGLQIATELCGFVTILSGTFLLHKTKD 299

Query: 321 M 322
           M
Sbjct: 304 M 299

BLAST of Lsi03G004650 vs. TrEMBL
Match: A0A0A0LMR9_CUCSA (Probable magnesium transporter OS=Cucumis sativus GN=Csa_2G402060 PE=3 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 1.7e-176
Identity = 333/355 (93.80%), Postives = 338/355 (95.21%), Query Frame = 1

Query: 1   MAVSGPASSSERAWSDSRKGMSSDNIKGLVLALSSSFFIGASFIVKKKGLKIAGASGVRA 60
           MAVSGPASSSER WSD RKGMSSDNIKGLVLALSSSFFIGASFIVKKKGLKIAGASGVRA
Sbjct: 1   MAVSGPASSSERLWSDPRKGMSSDNIKGLVLALSSSFFIGASFIVKKKGLKIAGASGVRA 60

Query: 61  GAGGYSYLYEPLWWVGMITMVIGEIANFAAYAFAPAILVTPLGALSIIIRQEDKLAALAH 120
           GAGGYSYLYEPLWWVGMITMV+GEIANFAAYAFAPAILVTPLGALSIII      AALAH
Sbjct: 61  GAGGYSYLYEPLWWVGMITMVVGEIANFAAYAFAPAILVTPLGALSIIIS-----AALAH 120

Query: 121 IILRERLNIFGILGCVLCVVGSTTIVLHAPQEREIESVKEVWDLATEPAFLLYAALMIAT 180
           IILRERLNIFGILGCVLCVVGSTTIVLHAPQEREI SVKEVWDLATEPAFLLYAA MIAT
Sbjct: 121 IILRERLNIFGILGCVLCVVGSTTIVLHAPQEREIVSVKEVWDLATEPAFLLYAAFMIAT 180

Query: 181 TLVLIIHFVPHYGQTYVMVYIGVCSIVGSLSVMCVKALGIALKLTFSGMNQLTYPQTWAF 240
           TL+LIIHFVP YGQTYVMVYIGVCSIVGSLSVM VKALGIALKLTF GMNQLTYPQTWAF
Sbjct: 181 TLILIIHFVPRYGQTYVMVYIGVCSIVGSLSVMSVKALGIALKLTFLGMNQLTYPQTWAF 240

Query: 241 TMFVITCVIIQINYLNKALDTFNTAVVSPTYYVMFTSLTILASIIMFKDWDRQSAIQIFT 300
           TM VITCVIIQ+NYLNKALDTFNTAVVSPTYYVMFT+LTILASIIMFKDWDRQ AIQIFT
Sbjct: 241 TMIVITCVIIQMNYLNKALDTFNTAVVSPTYYVMFTTLTILASIIMFKDWDRQGAIQIFT 300

Query: 301 QMCGFVTILAGTFLLHRTKDMVEASTTPSFSMRLSKHIEDGCELEAIPLQRQASL 356
           QMCGFVTILAGTFLLHRTKDMVEAS+TPSFSMR SKH EDGCELEAIPLQRQASL
Sbjct: 301 QMCGFVTILAGTFLLHRTKDMVEASSTPSFSMRPSKHTEDGCELEAIPLQRQASL 350

BLAST of Lsi03G004650 vs. TrEMBL
Match: A5C3H7_VITVI (Probable magnesium transporter OS=Vitis vinifera GN=VITISV_002232 PE=3 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 5.8e-148
Identity = 286/350 (81.71%), Postives = 311/350 (88.86%), Query Frame = 1

Query: 7   ASSSERAWSDSRKGMSSDNIKGLVLALSSSFFIGASFIVKKKGLKIAGASGVRAGAGGYS 66
           A+  E A+S   KGMS+DNIKGLVLALSSSFFIGASFIVKKKGLK AGASG+RAG GGYS
Sbjct: 3   AAGEEGAYS--YKGMSADNIKGLVLALSSSFFIGASFIVKKKGLKKAGASGIRAGVGGYS 62

Query: 67  YLYEPLWWVGMITMVIGEIANFAAYAFAPAILVTPLGALSIIIRQEDKLAALAHIILRER 126
           YLYEPLWWVGMITM++GEIANFAAYAFAPAILVTPLGALSIIIR  D  A LAHIILRE+
Sbjct: 63  YLYEPLWWVGMITMIVGEIANFAAYAFAPAILVTPLGALSIIIRH-DSNAVLAHIILREK 122

Query: 127 LNIFGILGCVLCVVGSTTIVLHAPQEREIESVKEVWDLATEPAFLLYAALMIATTLVLII 186
           L+IFGILGCVLCVVGSTTIVLHAPQEREI+SV EVWDLATEPAFL YAAL+I    VLII
Sbjct: 123 LHIFGILGCVLCVVGSTTIVLHAPQEREIQSVIEVWDLATEPAFLFYAALVITAVFVLII 182

Query: 187 HFVPHYGQTYVMVYIGVCSIVGSLSVMCVKALGIALKLTFSGMNQLTYPQTWAFTMFVIT 246
           HF+P YGQT++MVYIGVCS+VGSLSVM VKALGIALKLT SGMNQLTYPQTWAFT+ VIT
Sbjct: 183 HFIPQYGQTHIMVYIGVCSLVGSLSVMSVKALGIALKLTLSGMNQLTYPQTWAFTIVVIT 242

Query: 247 CVIIQINYLNKALDTFNTAVVSPTYYVMFTSLTILASIIMFKDWDRQSAIQIFTQMCGFV 306
           CVI Q+NYLNKALDTFNTAVVSP YYVMFTSLTILAS+IMFKDWDRQ+  QI T+MCGFV
Sbjct: 243 CVITQMNYLNKALDTFNTAVVSPIYYVMFTSLTILASVIMFKDWDRQNPTQIVTEMCGFV 302

Query: 307 TILAGTFLLHRTKDMVEASTTPSFSMRLSKHI-EDGCELEAIPLQRQASL 356
           TIL+GTFLLH+TKD+ +  +T S SMRLSKHI EDG   E IPL+RQ SL
Sbjct: 303 TILSGTFLLHKTKDLSDGLST-SLSMRLSKHIEEDGFGQEGIPLRRQESL 348

BLAST of Lsi03G004650 vs. TrEMBL
Match: A0A067LI84_JATCU (Probable magnesium transporter OS=Jatropha curcas GN=JCGZ_03916 PE=3 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 1.8e-146
Identity = 277/342 (80.99%), Postives = 306/342 (89.47%), Query Frame = 1

Query: 15  SDSRKGMSSDNIKGLVLALSSSFFIGASFIVKKKGLKIAGASGVRAGAGGYSYLYEPLWW 74
           +DS KGMSSDNIKGLVLALSSSFFIGASFIVKKKGL+ AG+SG+RAGAGGYSYLYEPLWW
Sbjct: 2   ADSYKGMSSDNIKGLVLALSSSFFIGASFIVKKKGLQKAGSSGLRAGAGGYSYLYEPLWW 61

Query: 75  VGMITMVIGEIANFAAYAFAPAILVTPLGALSIIIRQEDKLAALAHIILRERLNIFGILG 134
           +GMITM++GEIANFAAYAFAPAILVTPLGALSIII      AALAH+ILRERL+IFGILG
Sbjct: 62  IGMITMIVGEIANFAAYAFAPAILVTPLGALSIIIS-----AALAHLILRERLHIFGILG 121

Query: 135 CVLCVVGSTTIVLHAPQEREIESVKEVWDLATEPAFLLYAALMIATTLVLIIHFVPHYGQ 194
           CVLCVVGSTTIVLHAPQEREI+SVKEVWDLATEPAFL YA  +IA   ++I+H +PHYGQ
Sbjct: 122 CVLCVVGSTTIVLHAPQEREIQSVKEVWDLATEPAFLFYATCVIAAVFIIIVHIIPHYGQ 181

Query: 195 TYVMVYIGVCSIVGSLSVMCVKALGIALKLTFSGMNQLTYPQTWAFTMFVITCVIIQINY 254
           T+VMVYI VCS+VGSLSVM VKALGIALKLTFSGMNQL YPQTWAFT+FV TCVI Q+NY
Sbjct: 182 THVMVYIAVCSLVGSLSVMSVKALGIALKLTFSGMNQLVYPQTWAFTLFVATCVITQMNY 241

Query: 255 LNKALDTFNTAVVSPTYYVMFTSLTILASIIMFKDWDRQSAIQIFTQMCGFVTILAGTFL 314
           LNKALDTFNTAVVSP YYVMFTSLTILAS+IMFKDWDRQ+  QI T++CGFVTIL+GTFL
Sbjct: 242 LNKALDTFNTAVVSPIYYVMFTSLTILASVIMFKDWDRQNPTQIVTEICGFVTILSGTFL 301

Query: 315 LHRTKDMVEASTTPSFSMRLSKHIEDG-CELEAIPLQRQASL 356
           LH+TKDMVE S + S  +RLSKH E+   E E IPL+RQ SL
Sbjct: 302 LHKTKDMVEGS-SQSLPLRLSKHAEENPYEPEGIPLRRQESL 337

BLAST of Lsi03G004650 vs. TrEMBL
Match: F6HK54_VITVI (Probable magnesium transporter OS=Vitis vinifera GN=VIT_12s0035g00720 PE=3 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 2.4e-146
Identity = 284/350 (81.14%), Postives = 309/350 (88.29%), Query Frame = 1

Query: 7   ASSSERAWSDSRKGMSSDNIKGLVLALSSSFFIGASFIVKKKGLKIAGASGVRAGAGGYS 66
           A+  E A+S   KGMS+DNIKGLVLALSSSFFIGASFIVKKKGLK AGASG+RAG GGYS
Sbjct: 3   AAGEEGAYS--YKGMSADNIKGLVLALSSSFFIGASFIVKKKGLKKAGASGIRAGVGGYS 62

Query: 67  YLYEPLWWVGMITMVIGEIANFAAYAFAPAILVTPLGALSIIIRQEDKLAALAHIILRER 126
           YLYEPLWWVGMITM++GEIANFAAYAFAPAILVTPLGALSIII      A LAHIILRE+
Sbjct: 63  YLYEPLWWVGMITMIVGEIANFAAYAFAPAILVTPLGALSIIIS-----AVLAHIILREK 122

Query: 127 LNIFGILGCVLCVVGSTTIVLHAPQEREIESVKEVWDLATEPAFLLYAALMIATTLVLII 186
           L+IFGILGCVLCVVGSTTIVLHAPQEREI+SV EVWDLATEPAFL YAAL+I    VLII
Sbjct: 123 LHIFGILGCVLCVVGSTTIVLHAPQEREIQSVIEVWDLATEPAFLFYAALVITAVFVLII 182

Query: 187 HFVPHYGQTYVMVYIGVCSIVGSLSVMCVKALGIALKLTFSGMNQLTYPQTWAFTMFVIT 246
           HF+P YGQT++MVYIGVCS+VGSLSVM VKALGIALKLT SGMNQLTYPQTWAFT+ VIT
Sbjct: 183 HFIPQYGQTHIMVYIGVCSLVGSLSVMSVKALGIALKLTLSGMNQLTYPQTWAFTIVVIT 242

Query: 247 CVIIQINYLNKALDTFNTAVVSPTYYVMFTSLTILASIIMFKDWDRQSAIQIFTQMCGFV 306
           CVI Q+NYLNKALDTFNTAVVSP YYVMFTSLTILAS+IMFKDWDRQ+  QI T+MCGFV
Sbjct: 243 CVITQMNYLNKALDTFNTAVVSPIYYVMFTSLTILASVIMFKDWDRQNPTQIVTEMCGFV 302

Query: 307 TILAGTFLLHRTKDMVEASTTPSFSMRLSKHI-EDGCELEAIPLQRQASL 356
           TIL+GTFLLH+TKD+ +  +T S SMRLSKHI EDG   E IPL+RQ SL
Sbjct: 303 TILSGTFLLHKTKDLSDGLST-SLSMRLSKHIEEDGFGQEGIPLRRQESL 344

BLAST of Lsi03G004650 vs. TrEMBL
Match: M5XBW5_PRUPE (Probable magnesium transporter OS=Prunus persica GN=PRUPE_ppa007889mg PE=3 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 1.2e-145
Identity = 279/355 (78.59%), Postives = 301/355 (84.79%), Query Frame = 1

Query: 1   MAVSGPASSSERAWSDSRKGMSSDNIKGLVLALSSSFFIGASFIVKKKGLKIAGASGVRA 60
           MA  G   S   +W DS +GMSSDNIKGL+LALSSS FIGASFIVKKKGLK AGASG+RA
Sbjct: 1   MATEG--QSPPMSWRDSYRGMSSDNIKGLILALSSSLFIGASFIVKKKGLKKAGASGIRA 60

Query: 61  GAGGYSYLYEPLWWVGMITMVIGEIANFAAYAFAPAILVTPLGALSIIIRQEDKLAALAH 120
           G GGYSYLYEPLWWVGMITM++GEIANFAAYAFAPAILVTPLGALSIII      A LAH
Sbjct: 61  GVGGYSYLYEPLWWVGMITMIVGEIANFAAYAFAPAILVTPLGALSIIIS-----AVLAH 120

Query: 121 IILRERLNIFGILGCVLCVVGSTTIVLHAPQEREIESVKEVWDLATEPAFLLYAALMIAT 180
           IILRERL+IFGILGC LCVVGSTTIVLHAPQER IESV EVWDLA EP FLLYAAL+I  
Sbjct: 121 IILRERLHIFGILGCALCVVGSTTIVLHAPQERGIESVTEVWDLAMEPGFLLYAALVITA 180

Query: 181 TLVLIIHFVPHYGQTYVMVYIGVCSIVGSLSVMCVKALGIALKLTFSGMNQLTYPQTWAF 240
             +LI HF+P YGQT++MVYIGVCS+VGSLSVM VKALGIALKLT SGMNQL YPQTW F
Sbjct: 181 VFILIFHFIPQYGQTHIMVYIGVCSLVGSLSVMSVKALGIALKLTLSGMNQLVYPQTWVF 240

Query: 241 TMFVITCVIIQINYLNKALDTFNTAVVSPTYYVMFTSLTILASIIMFKDWDRQSAIQIFT 300
           T+ VITCV+ Q+NYLNKALDTFNTAVVSP YYVMFTSLTILAS+IMFKDWDRQS  Q+ T
Sbjct: 241 TLVVITCVLTQMNYLNKALDTFNTAVVSPIYYVMFTSLTILASVIMFKDWDRQSPTQVVT 300

Query: 301 QMCGFVTILAGTFLLHRTKDMVEASTTPSFSMRLSKHI-EDGCELEAIPLQRQAS 355
           +MCGFVTIL GTFLLHRTKDMV+   T   SMRLSKH  EDG + E IPL+RQ S
Sbjct: 301 EMCGFVTILGGTFLLHRTKDMVDGLPTSMSSMRLSKHADEDGIDGEGIPLRRQDS 348

BLAST of Lsi03G004650 vs. TAIR10
Match: AT1G71900.1 (AT1G71900.1 Protein of unknown function (DUF803))

HSP 1 Score: 499.6 bits (1285), Expect = 1.6e-141
Identity = 265/348 (76.15%), Postives = 297/348 (85.34%), Query Frame = 1

Query: 8   SSSERAWSDSRKGMSSDNIKGLVLALSSSFFIGASFIVKKKGLKIAGASGVRAGAGGYSY 67
           + S  +W DS KGMSSDNIKGLVLALSSS FIGASFIVKKKGLK A ++G RAG GGYSY
Sbjct: 2   AESSGSWRDSYKGMSSDNIKGLVLALSSSLFIGASFIVKKKGLKKAASTGTRAGVGGYSY 61

Query: 68  LYEPLWWVGMITMVIGEIANFAAYAFAPAILVTPLGALSIIIRQEDKLAALAHIILRERL 127
           LYEPLWW+GM TM++GEIANFAAYAFAPAILVTPLGA+SIII      A LAHIILRE+L
Sbjct: 62  LYEPLWWIGMTTMLLGEIANFAAYAFAPAILVTPLGAVSIIIS-----AVLAHIILREKL 121

Query: 128 NIFGILGCVLCVVGSTTIVLHAPQEREIESVKEVWDLATEPAFLLYAALMIATTLVLIIH 187
           +IFGILGC LCVVGSTTIVLHAPQEREI+SV EVW+LATEPAF+ YA+L+I   + LII 
Sbjct: 122 HIFGILGCALCVVGSTTIVLHAPQEREIDSVIEVWNLATEPAFMFYASLVIGAAVFLIIR 181

Query: 188 FVPHYGQTYVMVYIGVCSIVGSLSVMCVKALGIALKLTFSGMNQLTYPQTWAFTMFVITC 247
           FVP YGQT VMVYIG+CS+VGSLSVM VKALGIALKLTFSG NQL YPQTW FT+ V+TC
Sbjct: 182 FVPQYGQTNVMVYIGICSLVGSLSVMSVKALGIALKLTFSGTNQLFYPQTWIFTLVVLTC 241

Query: 248 VIIQINYLNKALDTFNTAVVSPTYYVMFTSLTILASIIMFKDWDRQSAIQIFTQMCGFVT 307
           V+ Q+NYLNKALDTFNTA+VSP YYVMFTSLTILAS+IMFKDWDRQ+  QI T++CGFVT
Sbjct: 242 VVTQLNYLNKALDTFNTAIVSPIYYVMFTSLTILASVIMFKDWDRQNGTQIVTEICGFVT 301

Query: 308 ILAGTFLLHRTKDMVEASTTPSFSMRLSKHIEDGCELEAIPLQRQASL 356
           IL+GTFLLHRTKDMVE S+     +R+SKHI    E E IPL+RQ SL
Sbjct: 302 ILSGTFLLHRTKDMVEGSSV-ILPLRISKHIN---EEEGIPLRRQESL 340

BLAST of Lsi03G004650 vs. TAIR10
Match: AT1G34470.1 (AT1G34470.1 Protein of unknown function (DUF803))

HSP 1 Score: 499.2 bits (1284), Expect = 2.1e-141
Identity = 262/345 (75.94%), Postives = 300/345 (86.96%), Query Frame = 1

Query: 8   SSSERAWSDSRKGMSSDNIKGLVLALSSSFFIGASFIVKKKGLKIAGASGVRAGAGGYSY 67
           +S   +W D+ KGMSSDNIKGLVLALSSS FIGASFIVKKKGLK AGASG+RAG+GGYSY
Sbjct: 2   ASLSGSWRDAYKGMSSDNIKGLVLALSSSLFIGASFIVKKKGLKRAGASGLRAGSGGYSY 61

Query: 68  LYEPLWWVGMITMVIGEIANFAAYAFAPAILVTPLGALSIIIRQEDKLAALAHIILRERL 127
           L EPLWWVGMITM++GEIANFAAYAFAPAILVTPLGALSIII      AALAH+IL E+L
Sbjct: 62  LLEPLWWVGMITMIVGEIANFAAYAFAPAILVTPLGALSIIIS-----AALAHVILHEKL 121

Query: 128 NIFGILGCVLCVVGSTTIVLHAPQEREIESVKEVWDLATEPAFLLYAALMIATTLVLIIH 187
           + FG+LGCVLCVVGS TIVLHAPQE+EI+SV +VW+LATEPAFLLYAA ++   ++LI+ 
Sbjct: 122 HTFGLLGCVLCVVGSITIVLHAPQEQEIDSVLQVWNLATEPAFLLYAAAVVGAAIILIVQ 181

Query: 188 FVPHYGQTYVMVYIGVCSIVGSLSVMCVKALGIALKLTFSGMNQLTYPQTWAFTMFVITC 247
           FVP YGQ++VMVYIGVCS+VGSLSVM VKALGIALKLTFSGMNQL YPQTW FT+ V+TC
Sbjct: 182 FVPQYGQSHVMVYIGVCSLVGSLSVMSVKALGIALKLTFSGMNQLIYPQTWVFTLIVLTC 241

Query: 248 VIIQINYLNKALDTFNTAVVSPTYYVMFTSLTILASIIMFKDWDRQSAIQIFTQMCGFVT 307
           VI Q+NYLNKALDTFNTAVVSP YYVMFTSLTILAS+IMFKDWDRQ   QI T++CGFVT
Sbjct: 242 VITQMNYLNKALDTFNTAVVSPIYYVMFTSLTILASVIMFKDWDRQDGTQIVTELCGFVT 301

Query: 308 ILAGTFLLHRTKDMVEASTT-PSFSMRLSKHIED--GCELEAIPL 350
           IL+GTFLLH+TKDMV+ S++  + ++RL K +ED  G E E IPL
Sbjct: 302 ILSGTFLLHKTKDMVDGSSSLGNLALRLPKQLEDSNGFEQEGIPL 341

BLAST of Lsi03G004650 vs. TAIR10
Match: AT4G09640.1 (AT4G09640.1 Protein of unknown function (DUF803))

HSP 1 Score: 478.0 bits (1229), Expect = 5.0e-135
Identity = 249/339 (73.45%), Postives = 286/339 (84.37%), Query Frame = 1

Query: 10  SERAWSDSRKGMSSDNIKGLVLALSSSFFIGASFIVKKKGLKIAGASGVRAGAGGYSYLY 69
           S  +W D+ KGMSSDN+KGLVLALSSS FIGASFIVKKKGLK AGASG+RAG+GGYSYL 
Sbjct: 4   SSGSWRDAYKGMSSDNVKGLVLALSSSIFIGASFIVKKKGLKKAGASGLRAGSGGYSYLL 63

Query: 70  EPLWWVGMITMVIGEIANFAAYAFAPAILVTPLGALSIIIRQEDKLAALAHIILRERLNI 129
           EPLWW+GMITM++GEIANFAAYAFAPAILVTPLGALSIII      A+LAHIIL+E+L+ 
Sbjct: 64  EPLWWIGMITMIVGEIANFAAYAFAPAILVTPLGALSIIIS-----ASLAHIILQEKLHT 123

Query: 130 FGILGCVLCVVGSTTIVLHAPQEREIESVKEVWDLATEPAFLLYAALMIATTLVLIIHFV 189
           FGILGC LC+VGS TIVLHAPQE++I SV EVW+LATEPAFL YAA ++   +VLI+ F+
Sbjct: 124 FGILGCALCIVGSVTIVLHAPQEQDIVSVLEVWNLATEPAFLFYAAAVVGAAIVLIVQFI 183

Query: 190 PHYGQTYVMVYIGVCSIVGSLSVMCVKALGIALKLTFSGMNQLTYPQTWAFTMFVITCVI 249
           P YGQ++VMVYIGVCS++GSLSVM VKALGIALKLTFSG NQL YPQTW FT+ V+ CVI
Sbjct: 184 PLYGQSHVMVYIGVCSLIGSLSVMSVKALGIALKLTFSGTNQLGYPQTWVFTVIVLFCVI 243

Query: 250 IQINYLNKALDTFNTAVVSPTYYVMFTSLTILASIIMFKDWDRQSAIQIFTQMCGFVTIL 309
            Q+NYLNKALDTFNTAVVSP YYVMFTSLTILAS+IMFKDWDRQS  QI T++CGFVTIL
Sbjct: 244 TQMNYLNKALDTFNTAVVSPIYYVMFTSLTILASVIMFKDWDRQSGTQIMTELCGFVTIL 303

Query: 310 AGTFLLHRTKDMVEASTTPSFS--------MRLSKHIED 341
           +GTFLLH T DMV+  +  + S        +R+ KH ED
Sbjct: 304 SGTFLLHTTTDMVDGESKGNLSSEEDSHLLLRIPKHSED 337

BLAST of Lsi03G004650 vs. TAIR10
Match: AT3G23870.1 (AT3G23870.1 Protein of unknown function (DUF803))

HSP 1 Score: 416.4 bits (1069), Expect = 1.8e-116
Identity = 211/313 (67.41%), Postives = 259/313 (82.75%), Query Frame = 1

Query: 21  MSSDNIKGLVLALSSSFFIGASFIVKKKGLKIAGASGVRAGAGGYSYLYEPLWWVGMITM 80
           MS DNI G++LA+SSS FIG+SFI+KKKGLK AGASGVRAG GGY YL EP WW GMITM
Sbjct: 4   MSPDNINGVILAVSSSIFIGSSFIIKKKGLKKAGASGVRAGEGGYGYLKEPWWWAGMITM 63

Query: 81  VIGEIANFAAYAFAPAILVTPLGALSIIIRQEDKLAALAHIILRERLNIFGILGCVLCVV 140
           ++GE+ANFAAYAFAPAILVTPLGALSII       A LAH IL+E+L++FGILGC+LCVV
Sbjct: 64  IVGEVANFAAYAFAPAILVTPLGALSIIFS-----AVLAHFILKEKLHMFGILGCILCVV 123

Query: 141 GSTTIVLHAPQEREIESVKEVWDLATEPAFLLYAALMIATTLVLIIHFVPHYGQTYVMVY 200
           GSTTIVLHAP E++IESVK++W LA EP FL+Y+A+++    +LI ++ P YG+T+++VY
Sbjct: 124 GSTTIVLHAPHEQKIESVKQIWQLAIEPGFLVYSAVIVIVVAILIFYYEPRYGKTHMIVY 183

Query: 201 IGVCSIVGSLSVMCVKALGIALKLTFSGMNQLTYPQTWAFTMFVITCVIIQINYLNKALD 260
           +G+CS++GSL+VM VKA+ IA+KLTFSG NQ  Y  TW F + V TC I+QINYLNKALD
Sbjct: 184 VGICSLMGSLTVMSVKAVAIAIKLTFSGTNQFKYFNTWIFILVVATCCILQINYLNKALD 243

Query: 261 TFNTAVVSPTYYVMFTSLTILASIIMFKDWDRQSAIQIFTQMCGFVTILAGTFLLHRTKD 320
           TFNTAV+SP YYVMFT+ TI+AS+IMFKDW  QS ++I T++CGFVTIL+GTFLLH+TKD
Sbjct: 244 TFNTAVISPVYYVMFTTFTIIASMIMFKDWASQSGLKIATELCGFVTILSGTFLLHKTKD 303

Query: 321 M-VEASTTPSFSM 333
           M   AS   S SM
Sbjct: 304 MGNSASGRGSISM 311

BLAST of Lsi03G004650 vs. TAIR10
Match: AT4G13800.1 (AT4G13800.1 Protein of unknown function (DUF803))

HSP 1 Score: 415.2 bits (1066), Expect = 4.0e-116
Identity = 210/301 (69.77%), Postives = 249/301 (82.72%), Query Frame = 1

Query: 21  MSSDNIKGLVLALSSSFFIGASFIVKKKGLKIAGASGVRAGAGGYSYLYEPLWWVGMITM 80
           MS DNI G++LA+SSS FIG+SFI+KKKGLK AG SG RAG GGY YLYEP WW GMITM
Sbjct: 4   MSPDNIHGVILAVSSSIFIGSSFIIKKKGLKKAGVSGARAGEGGYGYLYEPWWWAGMITM 63

Query: 81  VIGEIANFAAYAFAPAILVTPLGALSIIIRQEDKLAALAHIILRERLNIFGILGCVLCVV 140
           ++GEIANFAAYAFAPAILVTPLGALSII       A LAH IL E+L++FGILGCVLCVV
Sbjct: 64  IVGEIANFAAYAFAPAILVTPLGALSIIFS-----AVLAHFILEEKLHMFGILGCVLCVV 123

Query: 141 GSTTIVLHAPQEREIESVKEVWDLATEPAFLLYAALMIATTLVLIIHFVPHYGQTYVMVY 200
           GSTTIVLHAP E+ IESVK+VW LATEP FL Y+A+++   L LI ++ P YG+T+++VY
Sbjct: 124 GSTTIVLHAPHEQGIESVKQVWHLATEPGFLAYSAVVLVVVLALIFYYEPRYGKTHMIVY 183

Query: 201 IGVCSIVGSLSVMCVKALGIALKLTFSGMNQLTYPQTWAFTMFVITCVIIQINYLNKALD 260
           +G+CS++GSL+VM VKA+ IA+KLTFSGMNQ  Y   W F + V  C I+QINYLNKALD
Sbjct: 184 VGICSLMGSLTVMSVKAVAIAIKLTFSGMNQFKYFHAWIFIIVVTICCILQINYLNKALD 243

Query: 261 TFNTAVVSPTYYVMFTSLTILASIIMFKDWDRQSAIQIFTQMCGFVTILAGTFLLHRTKD 320
            FNTAV+SP YYVMFT+ TILAS+IMFKDW  QS +QI T++CGFVTIL+GTFLLH+TKD
Sbjct: 244 NFNTAVISPVYYVMFTTFTILASMIMFKDWASQSGLQIATELCGFVTILSGTFLLHKTKD 299

Query: 321 M 322
           M
Sbjct: 304 M 299

BLAST of Lsi03G004650 vs. NCBI nr
Match: gi|659081215|ref|XP_008441214.1| (PREDICTED: magnesium transporter NIPA2 [Cucumis melo])

HSP 1 Score: 631.7 bits (1628), Expect = 7.7e-178
Identity = 335/355 (94.37%), Postives = 340/355 (95.77%), Query Frame = 1

Query: 1   MAVSGPASSSERAWSDSRKGMSSDNIKGLVLALSSSFFIGASFIVKKKGLKIAGASGVRA 60
           MAVSGPASSSER WSD RKGMSSDNIKGLVLALSSSFFIGASFIVKKKGLKIAGASGVRA
Sbjct: 1   MAVSGPASSSERPWSDPRKGMSSDNIKGLVLALSSSFFIGASFIVKKKGLKIAGASGVRA 60

Query: 61  GAGGYSYLYEPLWWVGMITMVIGEIANFAAYAFAPAILVTPLGALSIIIRQEDKLAALAH 120
           GAGGYSYLYEPLWWVGMITMV+GEIANFAAYAFAPAILVTPLGALSIII      AALAH
Sbjct: 61  GAGGYSYLYEPLWWVGMITMVVGEIANFAAYAFAPAILVTPLGALSIIIS-----AALAH 120

Query: 121 IILRERLNIFGILGCVLCVVGSTTIVLHAPQEREIESVKEVWDLATEPAFLLYAALMIAT 180
           IILRERLNIFGILGCVLCVVGSTTIVLHAPQEREI SVKEVWDLATEPAFLLYAALMIAT
Sbjct: 121 IILRERLNIFGILGCVLCVVGSTTIVLHAPQEREIVSVKEVWDLATEPAFLLYAALMIAT 180

Query: 181 TLVLIIHFVPHYGQTYVMVYIGVCSIVGSLSVMCVKALGIALKLTFSGMNQLTYPQTWAF 240
           TL+LIIHFVP YGQTYVMVYIGVCSIVGSLSVM VKALGIALKLTFSG+NQLTYPQTWAF
Sbjct: 181 TLILIIHFVPRYGQTYVMVYIGVCSIVGSLSVMSVKALGIALKLTFSGLNQLTYPQTWAF 240

Query: 241 TMFVITCVIIQINYLNKALDTFNTAVVSPTYYVMFTSLTILASIIMFKDWDRQSAIQIFT 300
           TM VITCVIIQ+NYLNKALDTFNTAVVSPTYYVMFT+LTILASIIMFKDWDRQ AIQIFT
Sbjct: 241 TMIVITCVIIQMNYLNKALDTFNTAVVSPTYYVMFTTLTILASIIMFKDWDRQGAIQIFT 300

Query: 301 QMCGFVTILAGTFLLHRTKDMVEASTTPSFSMRLSKHIEDGCELEAIPLQRQASL 356
           QMCGFVTILAGTFLLHRTKDMVEAST PSFSMRLSKHIEDGCELEAIPLQR ASL
Sbjct: 301 QMCGFVTILAGTFLLHRTKDMVEASTAPSFSMRLSKHIEDGCELEAIPLQRHASL 350

BLAST of Lsi03G004650 vs. NCBI nr
Match: gi|778672954|ref|XP_004138651.2| (PREDICTED: probable magnesium transporter NIPA4 [Cucumis sativus])

HSP 1 Score: 626.7 bits (1615), Expect = 2.5e-176
Identity = 333/355 (93.80%), Postives = 338/355 (95.21%), Query Frame = 1

Query: 1   MAVSGPASSSERAWSDSRKGMSSDNIKGLVLALSSSFFIGASFIVKKKGLKIAGASGVRA 60
           MAVSGPASSSER WSD RKGMSSDNIKGLVLALSSSFFIGASFIVKKKGLKIAGASGVRA
Sbjct: 1   MAVSGPASSSERLWSDPRKGMSSDNIKGLVLALSSSFFIGASFIVKKKGLKIAGASGVRA 60

Query: 61  GAGGYSYLYEPLWWVGMITMVIGEIANFAAYAFAPAILVTPLGALSIIIRQEDKLAALAH 120
           GAGGYSYLYEPLWWVGMITMV+GEIANFAAYAFAPAILVTPLGALSIII      AALAH
Sbjct: 61  GAGGYSYLYEPLWWVGMITMVVGEIANFAAYAFAPAILVTPLGALSIIIS-----AALAH 120

Query: 121 IILRERLNIFGILGCVLCVVGSTTIVLHAPQEREIESVKEVWDLATEPAFLLYAALMIAT 180
           IILRERLNIFGILGCVLCVVGSTTIVLHAPQEREI SVKEVWDLATEPAFLLYAA MIAT
Sbjct: 121 IILRERLNIFGILGCVLCVVGSTTIVLHAPQEREIVSVKEVWDLATEPAFLLYAAFMIAT 180

Query: 181 TLVLIIHFVPHYGQTYVMVYIGVCSIVGSLSVMCVKALGIALKLTFSGMNQLTYPQTWAF 240
           TL+LIIHFVP YGQTYVMVYIGVCSIVGSLSVM VKALGIALKLTF GMNQLTYPQTWAF
Sbjct: 181 TLILIIHFVPRYGQTYVMVYIGVCSIVGSLSVMSVKALGIALKLTFLGMNQLTYPQTWAF 240

Query: 241 TMFVITCVIIQINYLNKALDTFNTAVVSPTYYVMFTSLTILASIIMFKDWDRQSAIQIFT 300
           TM VITCVIIQ+NYLNKALDTFNTAVVSPTYYVMFT+LTILASIIMFKDWDRQ AIQIFT
Sbjct: 241 TMIVITCVIIQMNYLNKALDTFNTAVVSPTYYVMFTTLTILASIIMFKDWDRQGAIQIFT 300

Query: 301 QMCGFVTILAGTFLLHRTKDMVEASTTPSFSMRLSKHIEDGCELEAIPLQRQASL 356
           QMCGFVTILAGTFLLHRTKDMVEAS+TPSFSMR SKH EDGCELEAIPLQRQASL
Sbjct: 301 QMCGFVTILAGTFLLHRTKDMVEASSTPSFSMRPSKHTEDGCELEAIPLQRQASL 350

BLAST of Lsi03G004650 vs. NCBI nr
Match: gi|1009137601|ref|XP_015886143.1| (PREDICTED: probable magnesium transporter NIPA4 [Ziziphus jujuba])

HSP 1 Score: 534.6 bits (1376), Expect = 1.3e-148
Identity = 278/343 (81.05%), Postives = 307/343 (89.50%), Query Frame = 1

Query: 13  AWSDSRKGMSSDNIKGLVLALSSSFFIGASFIVKKKGLKIAGASGVRAGAGGYSYLYEPL 72
           +W+DS KGMSSDNIKGL+LALSSSFFIGASFIVKKKGLK AGASGVRAG GGYSYL+EPL
Sbjct: 10  SWTDSYKGMSSDNIKGLILALSSSFFIGASFIVKKKGLKKAGASGVRAGVGGYSYLFEPL 69

Query: 73  WWVGMITMVIGEIANFAAYAFAPAILVTPLGALSIIIRQEDKLAALAHIILRERLNIFGI 132
           WWVGMITM++GEIANFAAYAFAPAILVTPLGALSIII      AALAHIIL E+L++FG+
Sbjct: 70  WWVGMITMIVGEIANFAAYAFAPAILVTPLGALSIIIS-----AALAHIILNEKLHVFGV 129

Query: 133 LGCVLCVVGSTTIVLHAPQEREIESVKEVWDLATEPAFLLYAALMIATTLVLIIHFVPHY 192
           LGCVLCVVGST+IVLHAPQEREIESVKEVWDLATEPAFLLYAA +IA  +++IIHF+P Y
Sbjct: 130 LGCVLCVVGSTSIVLHAPQEREIESVKEVWDLATEPAFLLYAAFVIAAVIIVIIHFIPQY 189

Query: 193 GQTYVMVYIGVCSIVGSLSVMCVKALGIALKLTFSGMNQLTYPQTWAFTMFVITCVIIQI 252
           GQT+VMVYIGVCS+VGSLSVM VKALGIALKLTFSGMNQL YPQTW FTM V+ CVI Q+
Sbjct: 190 GQTHVMVYIGVCSLVGSLSVMSVKALGIALKLTFSGMNQLVYPQTWVFTMVVLACVITQM 249

Query: 253 NYLNKALDTFNTAVVSPTYYVMFTSLTILASIIMFKDWDRQSAIQIFTQMCGFVTILAGT 312
           NYLNKALDTFNTAVVSP YYVMFTS TILAS+IMFKDWDRQ+A QI T+MCGFVTIL+GT
Sbjct: 250 NYLNKALDTFNTAVVSPIYYVMFTSFTILASVIMFKDWDRQNATQIMTEMCGFVTILSGT 309

Query: 313 FLLHRTKDMVEASTTPSFSMRLSKH-IEDGCELEAIPLQRQAS 355
           FLLH+TKDM E S+  S S R SKH IEDG  +EA+PL+ Q S
Sbjct: 310 FLLHKTKDMAEVSSPSSHSFRGSKHTIEDGFGIEAMPLRGQES 347

BLAST of Lsi03G004650 vs. NCBI nr
Match: gi|672181394|ref|XP_008810913.1| (PREDICTED: magnesium transporter NIPA2-like [Phoenix dactylifera])

HSP 1 Score: 532.3 bits (1370), Expect = 6.3e-148
Identity = 280/351 (79.77%), Postives = 310/351 (88.32%), Query Frame = 1

Query: 6   PASSSERAWSDSRKGMSSDNIKGLVLALSSSFFIGASFIVKKKGLKIAGASGVRAGAGGY 65
           P ++S R+W +S  GMS+DNIKGL+LALSSS FIGASFIVKKKGLK AGASGVRAG+GGY
Sbjct: 3   PGAASGRSWVESYTGMSADNIKGLILALSSSLFIGASFIVKKKGLKKAGASGVRAGSGGY 62

Query: 66  SYLYEPLWWVGMITMVIGEIANFAAYAFAPAILVTPLGALSIIIRQEDKLAALAHIILRE 125
           SYLYEPLWW+GMITMV+GE+ANFAAYAFAPAILVTPLGALSIII      AALAHIILRE
Sbjct: 63  SYLYEPLWWIGMITMVVGEVANFAAYAFAPAILVTPLGALSIIIS-----AALAHIILRE 122

Query: 126 RLNIFGILGCVLCVVGSTTIVLHAPQEREIESVKEVWDLATEPAFLLYAALMIATTLVLI 185
           RL+IFGILGCVLCVVGSTTIVLHAPQEREIESVKEVWDLATEPAFL YAA++IA   VLI
Sbjct: 123 RLHIFGILGCVLCVVGSTTIVLHAPQEREIESVKEVWDLATEPAFLFYAAVVIAAVFVLI 182

Query: 186 IHFVPHYGQTYVMVYIGVCSIVGSLSVMCVKALGIALKLTFSGMNQLTYPQTWAFTMFVI 245
            HFVPHYGQT++MVYIGVCS+VGSLSVM V+ALGIALKLTFSGMNQL YPQTWAFT+ V 
Sbjct: 183 FHFVPHYGQTHIMVYIGVCSLVGSLSVMSVQALGIALKLTFSGMNQLIYPQTWAFTIVVA 242

Query: 246 TCVIIQINYLNKALDTFNTAVVSPTYYVMFTSLTILASIIMFKDWDRQSAIQIFTQMCGF 305
            CVI Q+NYLNKALDTFNTAVVSP YYVMFTSLTILAS+IMFKDWDRQ+  QI T+MCGF
Sbjct: 243 ACVITQVNYLNKALDTFNTAVVSPIYYVMFTSLTILASVIMFKDWDRQNPTQIVTEMCGF 302

Query: 306 VTILAGTFLLHRTKDMVEASTTPSFSMRLSKHI-EDGCELEAIPLQRQASL 356
           +TIL+GTFLLH+TKDM +   +PS S+RL KH  EDG   E IPL+RQ S+
Sbjct: 303 ITILSGTFLLHKTKDMADGH-SPSLSVRLPKHADEDGYPPEGIPLRRQDSV 347

BLAST of Lsi03G004650 vs. NCBI nr
Match: gi|147855843|emb|CAN81783.1| (hypothetical protein VITISV_002232 [Vitis vinifera])

HSP 1 Score: 531.9 bits (1369), Expect = 8.3e-148
Identity = 286/350 (81.71%), Postives = 311/350 (88.86%), Query Frame = 1

Query: 7   ASSSERAWSDSRKGMSSDNIKGLVLALSSSFFIGASFIVKKKGLKIAGASGVRAGAGGYS 66
           A+  E A+S   KGMS+DNIKGLVLALSSSFFIGASFIVKKKGLK AGASG+RAG GGYS
Sbjct: 3   AAGEEGAYS--YKGMSADNIKGLVLALSSSFFIGASFIVKKKGLKKAGASGIRAGVGGYS 62

Query: 67  YLYEPLWWVGMITMVIGEIANFAAYAFAPAILVTPLGALSIIIRQEDKLAALAHIILRER 126
           YLYEPLWWVGMITM++GEIANFAAYAFAPAILVTPLGALSIIIR  D  A LAHIILRE+
Sbjct: 63  YLYEPLWWVGMITMIVGEIANFAAYAFAPAILVTPLGALSIIIRH-DSNAVLAHIILREK 122

Query: 127 LNIFGILGCVLCVVGSTTIVLHAPQEREIESVKEVWDLATEPAFLLYAALMIATTLVLII 186
           L+IFGILGCVLCVVGSTTIVLHAPQEREI+SV EVWDLATEPAFL YAAL+I    VLII
Sbjct: 123 LHIFGILGCVLCVVGSTTIVLHAPQEREIQSVIEVWDLATEPAFLFYAALVITAVFVLII 182

Query: 187 HFVPHYGQTYVMVYIGVCSIVGSLSVMCVKALGIALKLTFSGMNQLTYPQTWAFTMFVIT 246
           HF+P YGQT++MVYIGVCS+VGSLSVM VKALGIALKLT SGMNQLTYPQTWAFT+ VIT
Sbjct: 183 HFIPQYGQTHIMVYIGVCSLVGSLSVMSVKALGIALKLTLSGMNQLTYPQTWAFTIVVIT 242

Query: 247 CVIIQINYLNKALDTFNTAVVSPTYYVMFTSLTILASIIMFKDWDRQSAIQIFTQMCGFV 306
           CVI Q+NYLNKALDTFNTAVVSP YYVMFTSLTILAS+IMFKDWDRQ+  QI T+MCGFV
Sbjct: 243 CVITQMNYLNKALDTFNTAVVSPIYYVMFTSLTILASVIMFKDWDRQNPTQIVTEMCGFV 302

Query: 307 TILAGTFLLHRTKDMVEASTTPSFSMRLSKHI-EDGCELEAIPLQRQASL 356
           TIL+GTFLLH+TKD+ +  +T S SMRLSKHI EDG   E IPL+RQ SL
Sbjct: 303 TILSGTFLLHKTKDLSDGLST-SLSMRLSKHIEEDGFGQEGIPLRRQESL 348

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
NIPA4_ARATH2.8e-14076.15Probable magnesium transporter NIPA4 OS=Arabidopsis thaliana GN=At1g71900 PE=2 S... [more]
NIPA3_ARATH3.7e-14075.94Probable magnesium transporter NIPA3 OS=Arabidopsis thaliana GN=At1g34470 PE=2 S... [more]
NIPA5_ARATH8.9e-13473.45Probable magnesium transporter NIPA5 OS=Arabidopsis thaliana GN=At4g09640 PE=2 S... [more]
NIPA1_ARATH3.2e-11567.41Probable magnesium transporter NIPA1 OS=Arabidopsis thaliana GN=At3g23870 PE=2 S... [more]
NIPA2_ARATH7.0e-11569.77Probable magnesium transporter NIPA2 OS=Arabidopsis thaliana GN=At4g13800 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A0A0LMR9_CUCSA1.7e-17693.80Probable magnesium transporter OS=Cucumis sativus GN=Csa_2G402060 PE=3 SV=1[more]
A5C3H7_VITVI5.8e-14881.71Probable magnesium transporter OS=Vitis vinifera GN=VITISV_002232 PE=3 SV=1[more]
A0A067LI84_JATCU1.8e-14680.99Probable magnesium transporter OS=Jatropha curcas GN=JCGZ_03916 PE=3 SV=1[more]
F6HK54_VITVI2.4e-14681.14Probable magnesium transporter OS=Vitis vinifera GN=VIT_12s0035g00720 PE=3 SV=1[more]
M5XBW5_PRUPE1.2e-14578.59Probable magnesium transporter OS=Prunus persica GN=PRUPE_ppa007889mg PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G71900.11.6e-14176.15 Protein of unknown function (DUF803)[more]
AT1G34470.12.1e-14175.94 Protein of unknown function (DUF803)[more]
AT4G09640.15.0e-13573.45 Protein of unknown function (DUF803)[more]
AT3G23870.11.8e-11667.41 Protein of unknown function (DUF803)[more]
AT4G13800.14.0e-11669.77 Protein of unknown function (DUF803)[more]
Match NameE-valueIdentityDescription
gi|659081215|ref|XP_008441214.1|7.7e-17894.37PREDICTED: magnesium transporter NIPA2 [Cucumis melo][more]
gi|778672954|ref|XP_004138651.2|2.5e-17693.80PREDICTED: probable magnesium transporter NIPA4 [Cucumis sativus][more]
gi|1009137601|ref|XP_015886143.1|1.3e-14881.05PREDICTED: probable magnesium transporter NIPA4 [Ziziphus jujuba][more]
gi|672181394|ref|XP_008810913.1|6.3e-14879.77PREDICTED: magnesium transporter NIPA2-like [Phoenix dactylifera][more]
gi|147855843|emb|CAN81783.1|8.3e-14881.71hypothetical protein VITISV_002232 [Vitis vinifera][more]
The following terms have been associated with this gene:
Vocabulary: Cellular Component
TermDefinition
GO:0016021integral component of membrane
Vocabulary: Biological Process
TermDefinition
GO:0015693magnesium ion transport
Vocabulary: Molecular Function
TermDefinition
GO:0015095magnesium ion transmembrane transporter activity
Vocabulary: INTERPRO
TermDefinition
IPR008521Mg_trans_NIPA
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1903830 magnesium ion transmembrane transport
biological_process GO:0015693 magnesium ion transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005769 early endosome
cellular_component GO:0005886 plasma membrane
molecular_function GO:0015095 magnesium ion transmembrane transporter activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G004650.1Lsi03G004650.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008521Magnesium transporter NIPAPANTHERPTHR12570UNCHARACTERIZEDcoord: 10..354
score: 1.4E
IPR008521Magnesium transporter NIPAPFAMPF05653Mg_trans_NIPAcoord: 24..321
score: 2.1E
NoneNo IPR availablePANTHERPTHR12570:SF21SUBFAMILY NOT NAMEDcoord: 10..354
score: 1.4E
NoneNo IPR availableunknownSSF103481Multidrug resistance efflux transporter EmrEcoord: 71..149
score: 6.9