Lsi03G004360 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi03G004360
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionGroup IIA intron splicing facilitator CRS1
Locationchr03 : 4920202 .. 4921023 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTTCCGACAACTTCCTTCTCTCCAGCTCTACCAAGGATTCTCATTCCCCCATCCTTTCGATCAGATTCCACTCTCTTACACTCCACCCACAAGCCTATTTCCGCCACTTCTACACCATCACAATCATCTGTTCTATCCGAGTCGCCTTCCACCTCCAATGCCACCGTTAATCTCCGTACAGCGCCATGGATGAAGGGTCCTCTCCACCTTAAACCCCAACAAGAAGAAAGCGTGGATCTCGCAAAGCCCGAAAGAAGAAATGGGTCCGACGGTCATGGAGGGGATAAATGTTCTAGGGCTTTAGCTAATGGAAGAATTGATAAAACTGGGAAATACGCGATGCGGCGAATTGCTAAAAGCATCGGAAAGCTTCAGAAAAATGGGGATTCGGGGGAAACCCGAATGAAATTGGAGGAGGTTGAGTTTGGGGATTTTTGTTTGGAGGGTTTTGAGGAGTCTGGAACTCGCCGGAGAATGCCATGGGAGAAGGATGATGGGATTGTTTTGCGGAGGACTAAGAAGAAAACAGTGACTTCAGCGGAGTTGAATCTTGATAAAGTGTTGCTTGAAAGGTTGAGGGGTGAGGCTTCAAAAATGGAGAAATGGGTGAAGGTCAATAAAGTTGGGGTTACTCAAGATGTTGTGAATAAGATACAATTCATGTGGGAAAGAAATGAGCTTGCTATGCTGAAATTCGATGTGCCCTTGAGCCGCAATATGGATAGAGCACGAGAAATTGTTGAGGTAAGGTTGGTTTTGGTTAACTTTCTCTTTTGTATTCCAAGTATTTTGTTATTTGAAGTCCTTTCTACTTTGTAA

mRNA sequence

ATGCTTCCGACAACTTCCTTCTCTCCAGCTCTACCAAGGATTCTCATTCCCCCATCCTTTCGATCAGATTCCACTCTCTTACACTCCACCCACAAGCCTATTTCCGCCACTTCTACACCATCACAATCATCTGTTCTATCCGAGTCGCCTTCCACCTCCAATGCCACCGTTAATCTCCGTACAGCGCCATGGATGAAGGGTCCTCTCCACCTTAAACCCCAACAAGAAGAAAGCGTGGATCTCGCAAAGCCCGAAAGAAGAAATGGGTCCGACGGTCATGGAGGGGATAAATGTTCTAGGGCTTTAGCTAATGGAAGAATTGATAAAACTGGGAAATACGCGATGCGGCGAATTGCTAAAAGCATCGGAAAGCTTCAGAAAAATGGGGATTCGGGGGAAACCCGAATGAAATTGGAGGAGGTTGAGTTTGGGGATTTTTGTTTGGAGGGTTTTGAGGAGTCTGGAACTCGCCGGAGAATGCCATGGGAGAAGGATGATGGGATTGTTTTGCGGAGGACTAAGAAGAAAACAGTGACTTCAGCGGAGTTGAATCTTGATAAAGTGTTGCTTGAAAGGTTGAGGGGTGAGGCTTCAAAAATGGAGAAATGGGTGAAGGTCAATAAAGTTGGGGTTACTCAAGATGTTGTGAATAAGATACAATTCATGTGGGAAAGAAATGAGCTTGCTATGCTGAAATTCGATGTGCCCTTGAGCCGCAATATGGATAGAGCACGAGAAATTGTTGAGGTAAGGTTGGTTTTGGTTAACTTTCTCTTTTGTATTCCAAGTATTTTGTTATTTGAAGTCCTTTCTACTTTGTAA

Coding sequence (CDS)

ATGCTTCCGACAACTTCCTTCTCTCCAGCTCTACCAAGGATTCTCATTCCCCCATCCTTTCGATCAGATTCCACTCTCTTACACTCCACCCACAAGCCTATTTCCGCCACTTCTACACCATCACAATCATCTGTTCTATCCGAGTCGCCTTCCACCTCCAATGCCACCGTTAATCTCCGTACAGCGCCATGGATGAAGGGTCCTCTCCACCTTAAACCCCAACAAGAAGAAAGCGTGGATCTCGCAAAGCCCGAAAGAAGAAATGGGTCCGACGGTCATGGAGGGGATAAATGTTCTAGGGCTTTAGCTAATGGAAGAATTGATAAAACTGGGAAATACGCGATGCGGCGAATTGCTAAAAGCATCGGAAAGCTTCAGAAAAATGGGGATTCGGGGGAAACCCGAATGAAATTGGAGGAGGTTGAGTTTGGGGATTTTTGTTTGGAGGGTTTTGAGGAGTCTGGAACTCGCCGGAGAATGCCATGGGAGAAGGATGATGGGATTGTTTTGCGGAGGACTAAGAAGAAAACAGTGACTTCAGCGGAGTTGAATCTTGATAAAGTGTTGCTTGAAAGGTTGAGGGGTGAGGCTTCAAAAATGGAGAAATGGGTGAAGGTCAATAAAGTTGGGGTTACTCAAGATGTTGTGAATAAGATACAATTCATGTGGGAAAGAAATGAGCTTGCTATGCTGAAATTCGATGTGCCCTTGAGCCGCAATATGGATAGAGCACGAGAAATTGTTGAGGTAAGGTTGGTTTTGGTTAACTTTCTCTTTTGTATTCCAAGTATTTTGTTATTTGAAGTCCTTTCTACTTTGTAA

Protein sequence

MLPTTSFSPALPRILIPPSFRSDSTLLHSTHKPISATSTPSQSSVLSESPSTSNATVNLRTAPWMKGPLHLKPQQEESVDLAKPERRNGSDGHGGDKCSRALANGRIDKTGKYAMRRIAKSIGKLQKNGDSGETRMKLEEVEFGDFCLEGFEESGTRRRMPWEKDDGIVLRRTKKKTVTSAELNLDKVLLERLRGEASKMEKWVKVNKVGVTQDVVNKIQFMWERNELAMLKFDVPLSRNMDRAREIVEVRLVLVNFLFCIPSILLFEVLSTL
Homology
[Close]
BLAST of Lsi03G004360 vs. Swiss-Prot
Match: CRS1_ARATH (Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic OS=Arabidopsis thaliana GN=At5g16180 PE=3 SV=2)

HSP 1 Score: 151.4 bits (381), Expect = 1.5e-35
Identity = 99/221 (44.80%), Postives = 139/221 (62.90%), Query Frame = 1 

Query: 35  SATSTPSQSSVLSESPSTSNATVNLRTAPWMKGPLHLKPQQEESVDLAKPERRNGSDGHG 94
           S  +  SQ     E+   S+A + + TAPWMKGPL L+P  +E +D  K  +    +   
Sbjct: 59  SRRAKSSQFDQFRENRGVSDAAIKVPTAPWMKGPLLLRP--DEILDTKKRNKPRKVE--- 118

Query: 95  GDKCSRALANGRIDKTGKYAMRRIAKSIGKLQKNGDSGETRMK-LEEVEFGDFCLEGFEE 154
            +K  +AL        GK AM++I +++ KL ++ DS ET+M  L E E+     E  E 
Sbjct: 119 -EKTFKALNRRESGVRGKKAMKKIVRNVEKLDEDSDSEETQMDDLSEFEYLGRIEEKVES 178

Query: 155 SGT-RRRMPWEKDDG-IVLRRTKKKTV-TSAELNLDKVLLERLRGEASKMEKWVKVNKVG 214
                 +MPWE+++   +LRR KK++V T+AEL LD+ LL RLR EASKM KWV V K G
Sbjct: 179 KDRFGGKMPWEREEERFILRRMKKESVPTTAELILDEGLLNRLRREASKMRKWVNVRKAG 238

Query: 215 VTQDVVNKIQFMWERNELAMLKFDVPLSRNMDRAREIVEVR 252
           VT+ VVNKI+ MW+ NELAM++FDVPL RNM+RA+EI+E++
Sbjct: 239 VTELVVNKIKSMWKLNELAMVRFDVPLCRNMERAQEIIEMK 273

[Close]
BLAST of Lsi03G004360 vs. Swiss-Prot
Match: CRS1_ORYSJ (Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic OS=Oryza sativa subsp. japonica GN=Os08g0360100 PE=2 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 4.6e-13
Identity = 45/106 (42.45%), Postives = 65/106 (61.32%), Query Frame = 1 

Query: 152 EESGTRRRMPW----EKDDGIVLRRTKKKTV-TSAELNLDKVLLERLRGEASKMEKWVKV 211
           E+ G RR +PW    +++  +VLRR KK  V T AE  L+   LERLR  A   E+W + 
Sbjct: 117 EKKGIRRAVPWAAARDEETKVVLRREKKTRVPTRAETELEAGELERLRRAARGKERWARA 176

Query: 212 NKVGVTQDVVNKIQFMWER-NELAMLKFDVPLSRNMDRAREIVEVR 252
            K G+T +VV +++  W +  ELA ++   PL R MDRAREI+E++
Sbjct: 177 KKAGITDEVVEEVRGQWAKGQELAGVRIVEPLRRCMDRAREILEIK 222

[Close]
BLAST of Lsi03G004360 vs. Swiss-Prot
Match: CRS1_MAIZE (Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic OS=Zea mays GN=CRS1 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.4e-09
Identity = 42/105 (40.00%), Postives = 61/105 (58.10%), Query Frame = 1 

Query: 153 ESGTRRRMPWE--KDDG--IVLRRTKK-KTVTSAELNLDKVLLERLRGEASKMEKWVKVN 212
           E    R +PW   +D+G  + LRR KK +  T AE  L+   L RLR  A  + +W +  
Sbjct: 133 EKEKSRAVPWAAARDEGLKVALRREKKPREPTRAETELETHELRRLRRLARGIGRWARAK 192

Query: 213 KVGVTQDVVNKIQFMW-ERNELAMLKFDVPLSRNMDRAREIVEVR 252
           K GVT +VV +++  W    ELA ++   PL R+MDRAREI+E++
Sbjct: 193 KAGVTDEVVKEVRREWASGEELAAVRIVEPLRRSMDRAREILEIK 237

[Close]
BLAST of Lsi03G004360 vs. TrEMBL
Match: A0A0A0LSY1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G404830 PE=4 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 8.2e-110
Identity = 218/255 (85.49%), Postives = 229/255 (89.80%), Query Frame = 1 

Query: 1   MLPTTSFSPALPRILIPPSFRSDSTLLH-STHKPISATSTPSQSSVLSESPSTSNATVNL 60
           MLPTTSFS +LPR LIPPSFRS S LLH STH PISATSTPSQSSVL E PS SNA VNL
Sbjct: 1   MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNPISATSTPSQSSVLPEPPSISNAAVNL 60

Query: 61  RTAPWMKGPLHLKPQQ--EESVDLAKPERRNGSDGHGGDKCSRALANGRIDKTGKYAMRR 120
           RTAPWMK PLHL+PQQ  EE VD A P+RRNGSDG G DKCSRAL +  IDKTGKYAMRR
Sbjct: 61  RTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYAMRR 120

Query: 121 IAKSIGKLQKNGDSGETRMKLEEVEFGDFCLEGFEESGTRRRMPWEK-DDGIVLRRTKKK 180
           IAKSIGKL++NGD GETRMKLEEVEFG F LEGFEESGTRRRMPWEK DDGIVLRR KKK
Sbjct: 121 IAKSIGKLRRNGDLGETRMKLEEVEFGGFDLEGFEESGTRRRMPWEKDDDGIVLRRMKKK 180

Query: 181 TVTSAELNLDKVLLERLRGEASKMEKWVKVNKVGVTQDVVNKIQFMWERNELAMLKFDVP 240
           TVTSAELNLD+VLLERL+GEASKMEKWVKVNKVGVTQDVVN+IQFMWERNELAMLKFDVP
Sbjct: 181 TVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVP 240

Query: 241 LSRNMDRAREIVEVR 252
           LSRNMDRAREIVE++
Sbjct: 241 LSRNMDRAREIVEMK 255

[Close]
BLAST of Lsi03G004360 vs. TrEMBL
Match: B9RSN8_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1728180 PE=4 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 2.2e-38
Identity = 106/244 (43.44%), Postives = 148/244 (60.66%), Query Frame = 1 

Query: 22  SDSTLLHSTHKPISATSTPSQSSVLSESPSTSNATVNLRTAPWMKGPLHLKPQQEESVDL 81
           ++ + L++   P  AT+  ++ ++LS   S SNA + + TAPWMKGPL L+P   E ++L
Sbjct: 23  TNKSSLNNAQNPKFATNKNTEFTLLSVPNSQSNAPIKVPTAPWMKGPLLLQP--HELINL 82

Query: 82  AKPERRNGSDGHGGDKCSRALANGRIDKTGKYAMRRIAKSIGKLQKNGDSGETRMKLEEV 141
           +KP  +N S+    +K  + L        GK AM +I KSI +LQ+N    +T+   +  
Sbjct: 83  SKPRNKNSSNNANIEKSDKVLTGKESGVRGKKAMEKIVKSIEQLQENQALEKTQCDSQAY 142

Query: 142 EFGDFCLEGFE------------ESGTRRRM-PWEKDDGIVLRRTKK-KTVTSAELNLDK 201
           E      E FE            + G  +++ PWE+++  V  R KK K VT AEL L+K
Sbjct: 143 EKTQLDSEAFEIGEKLGLIREHGDFGVNKKLKPWEREEKFVYWRIKKEKAVTKAELILEK 202

Query: 202 VLLERLRGEASKMEKWVKVNKVGVTQDVVNKIQFMWERNELAMLKFDVPLSRNMDRAREI 252
            LLE LR EASKM KWVKV K GVTQ VV++I++ W  NELAM+KFD+PL RNMDRAREI
Sbjct: 203 ELLEILRTEASKMRKWVKVMKAGVTQSVVDQIRYAWRNNELAMVKFDLPLCRNMDRAREI 262

[Close]
BLAST of Lsi03G004360 vs. TrEMBL
Match: M5WXK2_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa016241mg PE=4 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 4.9e-38
Identity = 107/246 (43.50%), Postives = 145/246 (58.94%), Query Frame = 1 

Query: 34  ISATSTPSQSSVLSESPSTSNATVNLRTAPWMKGPLHLKPQQEESVDLAKPERRNGSDGH 93
           +S+T+T   S   SE  S+++A +   TAPWMKGPL L+P   E +D +KP  +   +  
Sbjct: 62  LSSTTTTPNSKAPSEPNSSTDACIKAPTAPWMKGPLLLQPH--EVIDFSKPRNKKTHNNA 121

Query: 94  GGDKCSRALANGRIDKTGKYAMRRIAKSIGKLQKNGDSGETRMKLEE------------- 153
             +K    LA   +   G  A+++I +SI +L  N  + ET+    E             
Sbjct: 122 KAEKPDTVLAGKLVGIRGDKAIKQIVQSIERLGPNQKTDETQKGFGEFRIWDSLEGLGQN 181

Query: 154 ----------VEFG-DFCLEGFEESGTRR---RMPWEKDDGIVLRRTKKKTVTSA-ELNL 213
                     VEFG   CLEG  ++   R   +MPWE+D+ IV +R KKK V SA EL+L
Sbjct: 182 EKWDETHKDFVEFGIGGCLEGLGKAADSRFGGKMPWERDERIVFQRIKKKRVASAAELSL 241

Query: 214 DKVLLERLRGEASKMEKWVKVNKVGVTQDVVNKIQFMWERNELAMLKFDVPLSRNMDRAR 252
           +K LLERLR EA+KM KWVKV K GVTQ +V+ I+F+W+ NELAM+KFDVPL RNM RA+
Sbjct: 242 EKELLERLRAEAAKMRKWVKVKKAGVTQAIVDDIKFIWKTNELAMVKFDVPLCRNMHRAQ 301

[Close]
BLAST of Lsi03G004360 vs. TrEMBL
Match: A0A161ZQS9_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_025905 PE=4 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 6.4e-38
Identity = 110/246 (44.72%), Postives = 147/246 (59.76%), Query Frame = 1 

Query: 16  IPPSFRSDSTLLHSTHKPISATSTPSQSSVLSESPSTSNATVNLRTAPWMK-GPL---HL 75
           IPP F   S   HS     +           S  P   N +  L T PWMK GP+    +
Sbjct: 23  IPPKFSFSSGFNHSKKDDKTRAKASDSDDSFSAQP---NLSTRLPTPPWMKRGPVLGDDI 82

Query: 76  KPQQEESVDLAKPERRNGSDGHGGDKCSRALANGRIDKTGKYAMRRIAKSIGKLQKN--G 135
           +P +         +R  G +  GG   ++ +  GR    GK AM +I +SIGKLQ+    
Sbjct: 83  EPSKLRKDRNFSNDR--GEENEGGQSLTKRVKGGR----GKKAMGKIFRSIGKLQETCLE 142

Query: 136 DSGETRMKLEEVEFGDFCLEGFEE---SGTRRRMPWEKDDGIVLRRTKK-KTVTSAELNL 195
           +S E R   ++++  DF + G EE   SG   +MPWE+D+ +  RR KK K VT+AEL+L
Sbjct: 143 ESEEFRKSPDQIKL-DFLMGGVEEDEFSGFGEQMPWERDERVFFRRMKKEKVVTAAELSL 202

Query: 196 DKVLLERLRGEASKMEKWVKVNKVGVTQDVVNKIQFMWERNELAMLKFDVPLSRNMDRAR 252
           D  LLERLRGEA+KM+KWVKV K GVT D+V++I+F+W+ NELAMLKFD+PL RNMDRAR
Sbjct: 203 DSGLLERLRGEAAKMKKWVKVKKAGVTWDIVSQIRFLWKNNELAMLKFDIPLCRNMDRAR 258

[Close]
BLAST of Lsi03G004360 vs. TrEMBL
Match: A0A103YGV6_CYNCS (RNA-binding, CRM domain-containing protein OS=Cynara cardunculus var. scolymus GN=Ccrd_012761 PE=4 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 8.3e-38
Identity = 106/245 (43.27%), Postives = 153/245 (62.45%), Query Frame = 1 

Query: 16  IPPSFRSDSTLLHSTHKPISATSTPSQSSVLSESPSTSNATVNLRTAPWMKGPLHLKPQQ 75
           +P    S +  +H  H  +S  +          +P+ SN+    +TAPWMKGPL L+P Q
Sbjct: 48  LPKKIHSSNPQIH--HSSLSVPTPEIHDKKHEFNPNPSNSAT--KTAPWMKGPLVLEPNQ 107

Query: 76  EESVDLAKPERRNGSDGHGGDK-CSRALANGRIDKTGKYAMRRIAKSIGKL-QKNGDSGE 135
              VD +KP+ RN S  +  +K  +  ++ GR    GK+AM++I  SI  L Q++ +  +
Sbjct: 108 --VVDFSKPKHRNKSSDNKDEKSLTHKVSGGR----GKHAMKKILYSIDNLEQESQELRD 167

Query: 136 TRMKLEEVEFG------DFCLEGFEESGTRRRMPWEKDDGIVLRRTKK-KTVTSAELNLD 195
           T+  L+++EF       D   +G ++   RR++PWE+++ +V RR KK K  T+AEL+LD
Sbjct: 168 TQNNLQQIEFDLSLGKLDEDSDGGDKRWFRRKLPWEREERMVFRRMKKEKVTTAAELSLD 227

Query: 196 KVLLERLRGEASKMEKWVKVNKVGVTQDVVNKIQFMWERNELAMLKFDVPLSRNMDRARE 252
             LLERLRGEA KM  WVKV KVGV + VV +I+ +W  +ELAMLKFD+PL RNMDRARE
Sbjct: 228 VRLLERLRGEARKMRNWVKVKKVGVNEGVVEQIRLIWNTDELAMLKFDMPLCRNMDRARE 282

[Close]
BLAST of Lsi03G004360 vs. TAIR10
Match: AT5G16180.1 (AT5G16180.1 ortholog of maize chloroplast splicing factor CRS1)

HSP 1 Score: 151.4 bits (381), Expect = 8.2e-37
Identity = 99/221 (44.80%), Postives = 139/221 (62.90%), Query Frame = 1 

Query: 35  SATSTPSQSSVLSESPSTSNATVNLRTAPWMKGPLHLKPQQEESVDLAKPERRNGSDGHG 94
           S  +  SQ     E+   S+A + + TAPWMKGPL L+P  +E +D  K  +    +   
Sbjct: 59  SRRAKSSQFDQFRENRGVSDAAIKVPTAPWMKGPLLLRP--DEILDTKKRNKPRKVE--- 118

Query: 95  GDKCSRALANGRIDKTGKYAMRRIAKSIGKLQKNGDSGETRMK-LEEVEFGDFCLEGFEE 154
            +K  +AL        GK AM++I +++ KL ++ DS ET+M  L E E+     E  E 
Sbjct: 119 -EKTFKALNRRESGVRGKKAMKKIVRNVEKLDEDSDSEETQMDDLSEFEYLGRIEEKVES 178

Query: 155 SGT-RRRMPWEKDDG-IVLRRTKKKTV-TSAELNLDKVLLERLRGEASKMEKWVKVNKVG 214
                 +MPWE+++   +LRR KK++V T+AEL LD+ LL RLR EASKM KWV V K G
Sbjct: 179 KDRFGGKMPWEREEERFILRRMKKESVPTTAELILDEGLLNRLRREASKMRKWVNVRKAG 238

Query: 215 VTQDVVNKIQFMWERNELAMLKFDVPLSRNMDRAREIVEVR 252
           VT+ VVNKI+ MW+ NELAM++FDVPL RNM+RA+EI+E++
Sbjct: 239 VTELVVNKIKSMWKLNELAMVRFDVPLCRNMERAQEIIEMK 273

[Close]
BLAST of Lsi03G004360 vs. TAIR10
Match: AT4G29750.1 (AT4G29750.1 CRS1 / YhbY (CRM) domain-containing protein)

HSP 1 Score: 56.6 bits (135), Expect = 2.8e-08
Identity = 32/78 (41.03%), Postives = 44/78 (56.41%), Query Frame = 1 

Query: 172 RTKKKTVTSAELNLDKVLLERLRGEASKMEKWVKVNKVGVTQDVVNKIQFMWERNELAML 231
           RT+K     AE  + +  L+RLR  A +M + VKV   G+TQ +V  I   WE +E+  L
Sbjct: 231 RTRKSNTVEAERIVPEHELKRLRNVALRMVERVKVGSAGITQALVEAIHEKWEVDEVVKL 290

Query: 232 KFDVPLSRNMDRAREIVE 250
           KF  P S NM R  E++E
Sbjct: 291 KFSEPYSLNMKRTHEVLE 308

[Close]
BLAST of Lsi03G004360 vs. TAIR10
Match: AT3G18390.1 (AT3G18390.1 CRS1 / YhbY (CRM) domain-containing protein)

HSP 1 Score: 56.2 bits (134), Expect = 3.6e-08
Identity = 35/105 (33.33%), Postives = 56/105 (53.33%), Query Frame = 1 

Query: 160 MPWEKDD------------GI-VLRRTKKKTVTSAELNLDKVLLERLRGEASKMEKWVKV 219
           +PWEK++            G+ V+++ + +  + AEL ++   L RLR +   +   + +
Sbjct: 205 LPWEKNEEEQAAERVVGEGGVAVMQKRRARAPSLAELTVEDSELRRLRRDGMYLRVRINI 264

Query: 220 NKVGVTQDVVNKIQFMWERNELAMLKFDVPLSRNMDRAREIVEVR 252
            K G+TQ V+ KI   W + EL  LKF   L+R+M  A EIVE R
Sbjct: 265 PKAGLTQAVMEKIYDTWRKEELVRLKFHEVLARDMKTAHEIVERR 309

[Close]
BLAST of Lsi03G004360 vs. NCBI nr
Match: gi|778673091|ref|XP_011649926.1| (PREDICTED: chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic [Cucumis sativus])

HSP 1 Score: 404.8 bits (1039), Expect = 1.2e-109
Identity = 218/255 (85.49%), Postives = 229/255 (89.80%), Query Frame = 1 

Query: 1   MLPTTSFSPALPRILIPPSFRSDSTLLH-STHKPISATSTPSQSSVLSESPSTSNATVNL 60
           MLPTTSFS +LPR LIPPSFRS S LLH STH PISATSTPSQSSVL E PS SNA VNL
Sbjct: 1   MLPTTSFSSSLPRSLIPPSFRSHSPLLHLSTHNPISATSTPSQSSVLPEPPSISNAAVNL 60

Query: 61  RTAPWMKGPLHLKPQQ--EESVDLAKPERRNGSDGHGGDKCSRALANGRIDKTGKYAMRR 120
           RTAPWMK PLHL+PQQ  EE VD A P+RRNGSDG G DKCSRAL +  IDKTGKYAMRR
Sbjct: 61  RTAPWMKAPLHLQPQQQEEEGVDPANPKRRNGSDGSGRDKCSRALGDSGIDKTGKYAMRR 120

Query: 121 IAKSIGKLQKNGDSGETRMKLEEVEFGDFCLEGFEESGTRRRMPWEK-DDGIVLRRTKKK 180
           IAKSIGKL++NGD GETRMKLEEVEFG F LEGFEESGTRRRMPWEK DDGIVLRR KKK
Sbjct: 121 IAKSIGKLRRNGDLGETRMKLEEVEFGGFDLEGFEESGTRRRMPWEKDDDGIVLRRMKKK 180

Query: 181 TVTSAELNLDKVLLERLRGEASKMEKWVKVNKVGVTQDVVNKIQFMWERNELAMLKFDVP 240
           TVTSAELNLD+VLLERL+GEASKMEKWVKVNKVGVTQDVVN+IQFMWERNELAMLKFDVP
Sbjct: 181 TVTSAELNLDRVLLERLKGEASKMEKWVKVNKVGVTQDVVNQIQFMWERNELAMLKFDVP 240

Query: 241 LSRNMDRAREIVEVR 252
           LSRNMDRAREIVE++
Sbjct: 241 LSRNMDRAREIVEMK 255

[Close]
BLAST of Lsi03G004360 vs. NCBI nr
Match: gi|659081301|ref|XP_008441260.1| (PREDICTED: chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic [Cucumis melo])

HSP 1 Score: 391.3 bits (1004), Expect = 1.3e-105
Identity = 208/253 (82.21%), Postives = 224/253 (88.54%), Query Frame = 1 

Query: 1   MLPTTSFSPALPRILIPPSFRSDSTLLH-STHKPISATSTPSQSSVLSESPSTSNATVNL 60
           MLPTTSFS  LP ILIPPSFRS S+LLH STH PISAT TPSQSSVLSE PS SNA +NL
Sbjct: 1   MLPTTSFSSPLPTILIPPSFRSHSSLLHLSTHHPISATFTPSQSSVLSEPPSISNAALNL 60

Query: 61  RTAPWMKGPLHLKPQQEESVDLAKPERRNGSDGHGGDKCSRALANGRIDKTGKYAMRRIA 120
           RTAPWMK PLHL+ QQEE VD A P+RRNGSD +GGDKCSRAL +  I+KTGKYAMRRIA
Sbjct: 61  RTAPWMKAPLHLQTQQEEGVDPANPKRRNGSDRNGGDKCSRALGDRGIEKTGKYAMRRIA 120

Query: 121 KSIGKLQKNGDSGETRMKLEEVEFGDFCLEGFEESGTRRRMPWEK-DDGIVLRRTKKKTV 180
           KSIGKL++NGD GETRM LEEVEFGDF LEGFEESGTRRRMPWEK DDGIV RR KKKT+
Sbjct: 121 KSIGKLRRNGDLGETRMNLEEVEFGDFDLEGFEESGTRRRMPWEKDDDGIVFRRMKKKTM 180

Query: 181 TSAELNLDKVLLERLRGEASKMEKWVKVNKVGVTQDVVNKIQFMWERNELAMLKFDVPLS 240
           TSAELNLD++LLERL+ +ASKMEKWVKVNKVGVTQDVVN IQ  WERNELAMLKFDVPLS
Sbjct: 181 TSAELNLDRLLLERLKAKASKMEKWVKVNKVGVTQDVVNNIQLKWERNELAMLKFDVPLS 240

Query: 241 RNMDRAREIVEVR 252
           RNMDRAREIVE++
Sbjct: 241 RNMDRAREIVEMK 253

[Close]
BLAST of Lsi03G004360 vs. NCBI nr
Match: gi|731417743|ref|XP_010660411.1| (PREDICTED: chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic isoform X1 [Vitis vinifera])

HSP 1 Score: 182.2 bits (461), Expect = 1.2e-42
Identity = 112/227 (49.34%), Postives = 150/227 (66.08%), Query Frame = 1 

Query: 33  PISATSTPSQS----SVLSESPSTSNATVNLRTAPWMKGPLHLKPQQEESVDLAKPERRN 92
           PISAT+T + +    S+ S+  S ++A + + TAPWMKGPL L+P +   +  A+P++  
Sbjct: 45  PISATTTATTNHPDHSISSQPVSGTDAAIKMPTAPWMKGPLLLQPNEVLDLSKARPKKVA 104

Query: 93  GSDGHGGDKCSRALANGRIDKTGKYAMRRIAKSIGKLQKNGDSGETRMKLEEVEFGDFCL 152
           GS   G +K  R+L        G  AM++I +SI KLQ+   S ET+   EE EFG   L
Sbjct: 105 GS--AGAEKPDRSLTEKVSGGRGAKAMKKIMQSIVKLQETHTSDETQENTEEFEFG-VSL 164

Query: 153 EGF---EESGTRRRMPWEKDDGIVLRRTKK-KTVTSAELNLDKVLLERLRGEASKMEKWV 212
           EG    E S    +MPW K + +V RRTKK K VT+AEL LD +LLERLRGEA KM KWV
Sbjct: 165 EGIGGDENSRIGGKMPWLKTEKVVFRRTKKEKVVTAAELTLDPMLLERLRGEAVKMRKWV 224

Query: 213 KVNKVGVTQDVVNKIQFMWERNELAMLKFDVPLSRNMDRAREIVEVR 252
           KV K GVT+ VV++I  +W+ +ELAM+KFD+PL RNMDRAREI+E++
Sbjct: 225 KVKKAGVTESVVDQIHMVWKSDELAMVKFDMPLCRNMDRAREILEIK 268

[Close]
BLAST of Lsi03G004360 vs. NCBI nr
Match: gi|731417747|ref|XP_010660413.1| (PREDICTED: chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic isoform X2 [Vitis vinifera])

HSP 1 Score: 182.2 bits (461), Expect = 1.2e-42
Identity = 112/227 (49.34%), Postives = 150/227 (66.08%), Query Frame = 1 

Query: 33  PISATSTPSQS----SVLSESPSTSNATVNLRTAPWMKGPLHLKPQQEESVDLAKPERRN 92
           PISAT+T + +    S+ S+  S ++A + + TAPWMKGPL L+P +   +  A+P++  
Sbjct: 45  PISATTTATTNHPDHSISSQPVSGTDAAIKMPTAPWMKGPLLLQPNEVLDLSKARPKKVA 104

Query: 93  GSDGHGGDKCSRALANGRIDKTGKYAMRRIAKSIGKLQKNGDSGETRMKLEEVEFGDFCL 152
           GS   G +K  R+L        G  AM++I +SI KLQ+   S ET+   EE EFG   L
Sbjct: 105 GS--AGAEKPDRSLTEKVSGGRGAKAMKKIMQSIVKLQETHTSDETQENTEEFEFG-VSL 164

Query: 153 EGF---EESGTRRRMPWEKDDGIVLRRTKK-KTVTSAELNLDKVLLERLRGEASKMEKWV 212
           EG    E S    +MPW K + +V RRTKK K VT+AEL LD +LLERLRGEA KM KWV
Sbjct: 165 EGIGGDENSRIGGKMPWLKTEKVVFRRTKKEKVVTAAELTLDPMLLERLRGEAVKMRKWV 224

Query: 213 KVNKVGVTQDVVNKIQFMWERNELAMLKFDVPLSRNMDRAREIVEVR 252
           KV K GVT+ VV++I  +W+ +ELAM+KFD+PL RNMDRAREI+E++
Sbjct: 225 KVKKAGVTESVVDQIHMVWKSDELAMVKFDMPLCRNMDRAREILEIK 268

[Close]
BLAST of Lsi03G004360 vs. NCBI nr
Match: gi|645279076|ref|XP_008244532.1| (PREDICTED: chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic [Prunus mume])

HSP 1 Score: 169.1 bits (427), Expect = 1.1e-38
Identity = 109/246 (44.31%), Postives = 146/246 (59.35%), Query Frame = 1 

Query: 34  ISATSTPSQSSVLSESPSTSNATVNLRTAPWMKGPLHLKPQQEESVDLAKPERRNGSDGH 93
           +S+T+T   S   SE  S+++A +   TAPWMKGPL L+P   E +D +KP  +   +  
Sbjct: 62  LSSTTTTPNSKAPSEPNSSTDACIKAPTAPWMKGPLLLQPH--EVIDFSKPRNKKTHNNA 121

Query: 94  GGDKCSRALANGRIDKTGKYAMRRIAKSIGKLQKNGDSGETRMKLEE------------- 153
             +K    LA   +   G  A+++I +SI +L  N  + ET+    E             
Sbjct: 122 KAEKPDTVLAGKLVGIRGDKAIKQIVQSIERLGPNQKTDETQKGFGEFRIWDSLEGLGQN 181

Query: 154 ----------VEFG-DFCLEGFEESGTRR---RMPWEKDDGIVLRRTKKKTVTSA-ELNL 213
                     VEFG   CLEG  ++   R   +MPWE+D+ IV +R KKK V SA EL+L
Sbjct: 182 EKWDETHKDFVEFGIGGCLEGLGKAAESRFGGKMPWERDERIVFQRIKKKRVASAAELSL 241

Query: 214 DKVLLERLRGEASKMEKWVKVNKVGVTQDVVNKIQFMWERNELAMLKFDVPLSRNMDRAR 252
           +K LLERLRGEA+KM KWVKV K GVTQ VV+ I+F+W+ NELAM+KFDVPL RNM RA+
Sbjct: 242 EKELLERLRGEAAKMRKWVKVKKAGVTQAVVDDIKFIWKTNELAMVKFDVPLCRNMHRAQ 301

The following BLAST results are available for this feature:
BLAST of Lsi03G004360 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CRS1_ARATH1.5e-3544.80Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic OS=Arabi... [more]
CRS1_ORYSJ4.6e-1342.45Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic OS=Oryza... [more]
CRS1_MAIZE1.4e-0940.00Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic OS=Zea m... [more]
BLAST of Lsi03G004360 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LSY1_CUCSA8.2e-11085.49Uncharacterized protein OS=Cucumis sativus GN=Csa_2G404830 PE=4 SV=1[more]
B9RSN8_RICCO2.2e-3843.44Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1728180 PE=4 SV=1[more]
M5WXK2_PRUPE4.9e-3843.50Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa016241mg PE=4 SV=1[more]
A0A161ZQS9_DAUCA6.4e-3844.72Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_025905 PE=4 SV=1[more]
A0A103YGV6_CYNCS8.3e-3843.27RNA-binding, CRM domain-containing protein OS=Cynara cardunculus var. scolymus G... [more]
BLAST of Lsi03G004360 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G16180.18.2e-3744.80 ortholog of maize chloroplast splicing factor CRS1[more]
AT4G29750.12.8e-0841.03 CRS1 / YhbY (CRM) domain-containing protein[more]
AT3G18390.13.6e-0833.33 CRS1 / YhbY (CRM) domain-containing protein[more]
BLAST of Lsi03G004360 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778673091|ref|XP_011649926.1|1.2e-10985.49PREDICTED: chloroplastic group IIA intron splicing facilitator CRS1, chloroplast... [more]
gi|659081301|ref|XP_008441260.1|1.3e-10582.21PREDICTED: chloroplastic group IIA intron splicing facilitator CRS1, chloroplast... [more]
gi|731417743|ref|XP_010660411.1|1.2e-4249.34PREDICTED: chloroplastic group IIA intron splicing facilitator CRS1, chloroplast... [more]
gi|731417747|ref|XP_010660413.1|1.2e-4249.34PREDICTED: chloroplastic group IIA intron splicing facilitator CRS1, chloroplast... [more]
gi|645279076|ref|XP_008244532.1|1.1e-3844.31PREDICTED: chloroplastic group IIA intron splicing facilitator CRS1, chloroplast... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0003723RNA binding
Vocabulary: INTERPRO
TermDefinition
IPR001890RNA-binding_CRM
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003723 RNA binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G004360.1Lsi03G004360.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001890RNA-binding, CRM domainGENE3DG3DSA:3.30.110.60coord: 189..249
score: 6.
IPR001890RNA-binding, CRM domainPFAMPF01985CRS1_YhbYcoord: 190..251
score: 4.0
IPR001890RNA-binding, CRM domainPROFILEPS51295CRMcoord: 183..273
score: 11
IPR001890RNA-binding, CRM domainunknownSSF75471YhbY-likecoord: 185..249
score: 3.9
NoneNo IPR availablePANTHERPTHR31846FAMILY NOT NAMEDcoord: 158..251
score: 4.7
NoneNo IPR availablePANTHERPTHR31846:SF10CHLOROPLASTIC GROUP IIA INTRON SPLICING FACILITATOR CRS1, CHLOROPLASTICcoord: 158..251
score: 4.7

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi03G004360Cucurbita maxima (Rimu)cmalsiB276
Lsi03G004360Cucurbita maxima (Rimu)cmalsiB656
Lsi03G004360Cucurbita moschata (Rifu)cmolsiB265
Lsi03G004360Cucurbita moschata (Rifu)cmolsiB645
Lsi03G004360Cucurbita pepo (Zucchini)cpelsiB143
Lsi03G004360Cucurbita pepo (Zucchini)cpelsiB339
Lsi03G004360Wild cucumber (PI 183967)cpilsiB113
Lsi03G004360Cucumber (Chinese Long) v2culsiB115
Lsi03G004360Melon (DHL92) v3.5.1lsimeB259
Lsi03G004360Watermelon (Charleston Gray)lsiwcgB227
Lsi03G004360Watermelon (97103) v1lsiwmB275
Lsi03G004360Cucumber (Gy14) v2cgyblsiB102
Lsi03G004360Melon (DHL92) v3.6.1lsimedB272
Lsi03G004360Silver-seed gourdcarlsiB017
Lsi03G004360Silver-seed gourdcarlsiB986
Lsi03G004360Cucumber (Chinese Long) v3cuclsiB127
Lsi03G004360Watermelon (97103) v2lsiwmbB224
Lsi03G004360Wax gourdlsiwgoB322
Lsi03G004360Cucumber (Gy14) v1cgylsiB098