Lsi03G002200 (gene) Bottle gourd (USVL1VR-Ls)

BLAST of Lsi03G002200 vs. Swiss-Prot
Match: ENG1_ARTBC (Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) GN=ARB_01444 PE=1 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 4.5e-39
Identity = 164/580 (28.28%), Postives = 263/580 (45.34%), Query Frame = 1

Query: 174 SGNLRFFLVRGSPFMTFEVLNNTPISISTIHAIL------SFSSNSSLTKFTVTLNNNQT 233
           S ++ F LV+G  F+T  + NN   +I +  A+L      + S    + K+ +TL +++ 
Sbjct: 335 SESITFPLVQGMGFIT-AIYNNLQPAIQS--AVLFRKVEPAGSPQGGIFKYKITLEDDKN 394

Query: 234 WLIY----SSSPINLTHSLSQITSG--GFSGIIRIAVLPNPH-CETILDRFSSCYP---- 293
           WL+Y    + +   L    +++ SG  GF G+I++A  P+    E I D+ +  Y     
Sbjct: 395 WLLYVIPENGADPKLKLEGNKLISGPTGFKGVIQVAKNPSAEEGEGIYDKSAGSYATDIK 454

Query: 294 VSGEVNFRNPFCLEYKWEKNGWGN-LLMLAHPLHLRLLSGYDSGAVVLDDFKYKSID-GD 353
           +SG V        ++ +EK G G  L+M A P H+      D+      + K  +   G 
Sbjct: 455 ISGSVGTDGTGTYKFSFEKAGKGAPLVMYALPHHVESFD--DATKNTKKNMKLSTTTKGM 514

Query: 354 LVGIVGGSWVLKPD--PVSVTWH--SINGVGEEFQSEIISALVKDVEGLKSSP-----IT 413
               VG SW +     P+S+ +         +   SE     +K V G + S        
Sbjct: 515 ATACVGDSWTMVEGNLPLSMDFAPWKPGSSSQVTLSEGAKNAIKAVAGNELSQDMELQTN 574

Query: 414 TTSSYFYGKSIAR-AARLALIAEEVNFLQVIPEVRKFLKGAIEPWLHGTFNGNGFLYDGK 473
             S YF GK + + A  +  + E V            LK + + ++         +YD  
Sbjct: 575 LNSMYFSGKGLNKFAGAIYTVQELVGDKAAASGPLNSLKESFKRFVDNK-QQIPLVYDNV 634

Query: 474 WGGLVT----QQGSTNSGADFGFGVYNDHHYHIGYFLYAIAVLVKIDPAWGRKFRPHAYS 533
           W G+V+    ++G T  G DFG  +YNDHH+H GYF+   A+L K+DPAW    + +   
Sbjct: 635 WKGVVSSGTYEKGDT--GLDFGNTLYNDHHFHYGYFILTAAILGKLDPAWLDANKAYVNM 694

Query: 534 LMADFMNLSRRSNSTFPRLRCFDLYKLHSWASGLTEFADGRNQESTSEAVNAYYSAALLG 593
           L+ D  N S  ++  FP  R FD Y  HSWA GL E +DG++QESTSE     Y+  + G
Sbjct: 695 LVRDSGN-SVDNDEHFPFSRAFDWYHGHSWAKGLFESSDGKDQESTSEDTMYAYAIKMWG 754

Query: 594 LAYGDTHLASIGSTLTALEIKAAQTWWQIREGDNLYDVDFARENKVVGVLWSNKRDSGLW 653
              GD  + + G+ +  +  +    ++ + + DN+        NKV G+L+ NK D   +
Sbjct: 755 KTSGDKSMEARGNLMLGILARTLNNYF-LMKNDNVNQPKNFIGNKVTGILFENKIDHTTY 814

Query: 654 FAPPDWRECRLGIQVLPLLPITEILFSDAGFAKELVDWTL-----PSLGREGVGEGWKGF 712
           F      E   GI +LPLLP +    S A F KE  +W        +   E V  GWKG 
Sbjct: 815 FGAN--LEYIQGIHMLPLLPNSAYTRS-AEFVKE--EWEAMFASGAAAPAEKVTGGWKGV 874

BLAST of Lsi03G002200 vs. Swiss-Prot
Match: ENG2_SCHPO (Putative endo-1,3(4)-beta-glucanase 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=eng2 PE=3 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 4.2e-37
Identity = 181/678 (26.70%), Postives = 296/678 (43.66%), Query Frame = 1

Query: 61  PVAALFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIK---- 120
           P+  +FP   S   P P      +L   P+ TN F+ N  +     P + HPY +     
Sbjct: 11  PINPVFP---SRAHPSPPLGLTSNLF--PIQTNKFYGNLYIGTRHNPSWSHPYSVTWLNG 70

Query: 121 SSLSTVSISYPS----------------MFLNSAFGYQVF-NADWTVSENPSSVSQKPHI 180
           SS   ++IS+                   + N A  Y +  +A    S N  S+ Q  H 
Sbjct: 71  SSYYGLAISHIDDSQRVFGPDPESVPCQYYFNPAGLYSIIISAREFASGNLLSLDQSRHF 130

Query: 181 ISSFSDLSLTLDIPSGNLRFFLVRGSPFMTFEVLNNTPISISTIHAILSFSSNSSLT--- 240
            S  + LS T    SG +   +V G  F++    N TP+  S+I     FSS + +    
Sbjct: 131 -SIQATLSATTS-GSGTIILPIVAGMGFVSGYYTNLTPVFNSSI----LFSSITKINFSR 190

Query: 241 --KFTVTLNNNQTWLIY-----SSSPINLT-HSLSQIT-SGGFSGIIRIAVLPNP----- 300
             K+ + L + + W +Y     SSS  NLT  S SQ+T S  F+G+I+I  +PN      
Sbjct: 191 GYKYRIQLTDGKIWFLYAFPTSSSSTFNLTLASNSQLTTSTKFTGLIQICKVPNESVNNS 250

Query: 301 HCETILDRFSSCYPVSGEVNFRNPFCLEYKWEK---NGWGNL--LMLAHPLHLRLLSGYD 360
           + +TI D  +  Y  S  ++ +        W +    G+ NL  LM A P H++   G D
Sbjct: 251 YPDTIYDASAGVYTTSISLSAQVSGTTGEYWFRFATAGYTNLNPLMFALPHHMQSF-GSD 310

Query: 361 SGAVVLDDFKYKSIDGDLVGIVGGSWVL--KPDPVSVTWHSI--NGVGEEFQSEIISAL- 420
           + A         +  G +      +W L  K  P  V +  I  NG    +    ++A+ 
Sbjct: 311 TQAYKTGLGLASTTMGIMFAYATKTWHLIEKNLPTQVGFLPIPWNGGSNTYSPTALAAIR 370

Query: 421 ---VKDVEGLKSSPITTTSSYFYGKSIARAARLALIAEEVNFLQVIPEVRKF-LKGAIEP 480
                D+     +     S Y  GK +A  A++ L+A  +     +       LK A+  
Sbjct: 371 AACATDINFDVVNASNLDSMYTSGKIVAMYAQVCLVASRILGDSTLTNTGLTKLKQAMAR 430

Query: 481 WLHGTFNGNGFLYDGKWGGLVTQQGSTNSGADFGFGVYNDHHYHIGYFLYAIAVLVKIDP 540
           +   T      +YD  + G+++  G ++  AD+G   YNDHH+H GY +YA AV+  +DP
Sbjct: 431 FTTNT-QMYPLVYDTTYKGIISTAGYSSPLADYGNTYYNDHHFHWGYHIYACAVIGLLDP 490

Query: 541 AW--GRKFRPHAYSLMADFMNLSRRSNSTFPRLRCFDLYKLHSWASGLTEFADGRNQEST 600
           +W      R +  +L+ D  N S  S++ F   R FD +  HSWA+G+ E  DG+++EST
Sbjct: 491 SWLVNDNIR-YVNALLRDSANPS-ESDTYFAMFRNFDWFVGHSWATGIFESGDGKDEEST 550

Query: 601 SEAVNAYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIREGDNLYDVDFARENKV 660
           SE  N  Y+  L G+   DT L +  + + A+   +  T+  +    ++        N V
Sbjct: 551 SEDFNFLYATKLWGMVRNDTVLINRANLMLAVLKNSLNTYIYMTPTTSVQPSQIL-GNYV 610

Query: 661 VGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEILFSDAGFAKELVDWTLPSLGRE 685
            G+ + NK D   +F+  ++  C+ GI ++P  PI+  L S +   ++      P +   
Sbjct: 611 TGITFMNKVDYATYFSAEEY--CKQGIHMIPTTPISGYLRSPSYVQQDWNAKIAPIINT- 668

BLAST of Lsi03G002200 vs. Swiss-Prot
Match: ENG1_SCHPO (Endo-1,3(4)-beta-glucanase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=eng1 PE=3 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 2.3e-35
Identity = 177/672 (26.34%), Postives = 277/672 (41.22%), Query Frame = 1

Query: 86  LSSPLPTNSFFQNFTVKNGDQPEYIHP--YLIKSSLSTVSISYPSMFLNSAFGYQVFNAD 145
           LSSP+ TN FF N  + +     Y  P  Y  +SS +   I       N     +V + D
Sbjct: 78  LSSPIETNKFFDNNLLGSRTNFMYADPFRYWWQSSDTMGGICIAHTDDNQ----RVMDTD 137

Query: 146 WTVSE---NPSSV-----------SQKPHIISSFSDLS--LTLDIPSGNLRFFLVRGSPF 205
            T+      P  +           S    I+     +S   T    S +++  L  G   
Sbjct: 138 DTIPSYYYEPIGICSLGFGASGITSNTDPIVDEIDQMSARFTFSWDSSSMQLTLTEGMAV 197

Query: 206 MTFEVLNNTPISIST---IHAILSFSSNSSLTKFTVTLNNNQTWLIYS-SSPINLTHSLS 265
            T    N  P   S+   I+  +     S++ K+ VT+++N  WLIY     + LT S S
Sbjct: 198 TTAVYTNAIPQIFSSTLYINDFVEVPGTSAVQKYRVTMSDNHVWLIYIYGDSLTLTESTS 257

Query: 266 QITSGG--FSGIIRIAVLP--NPHCETILDRFSSCY----PVSGEVNFRNPFCLEYKWEK 325
           Q+  G   F+G I+IA +P  +   E + D ++  Y     +SG V     +   Y ++ 
Sbjct: 258 QMLVGSNTFNGYIQIAKIPLGDGTAEALYDTYAGVYITGISISGYVEDAVGY---YSFDF 317

Query: 326 NGWGNLLMLAHPLHLRLLSGYD---SGAVVLDDFKYKSIDGDLVGIVGGSWVLK---PDP 385
              G+  +   PL   L    D   SG  V        + GD+    G S       P  
Sbjct: 318 TTAGDTSV--EPLFFLLPHQVDTAVSGTKVTSIVLASLVSGDMNAAAGNSITFAEAIPQD 377

Query: 386 VSVTWHSINGVGEEFQSEIISALVKDVEGLK-----SSPITTTSSYFYGKSIARAARLA- 445
           +     S  G G+   SE    ++ +V G +     S+     S Y+ GK +A+ A L  
Sbjct: 378 IGFLPWSPTG-GQIGYSEEALEIIAEVAGTELGEDFSANSNLNSMYYSGKVLAKYAMLCV 437

Query: 446 ----LIAEEVNFLQVIPEVRKFLKGAIEPWLHGTFNGNGFLYDGKWGGLVTQQG-STNSG 505
               ++ +E +  Q I ++       ++             YD  W G+V+  G S +S 
Sbjct: 438 TINDILGDETSSEQCIQKLEAAFARFVD-----NQQIYPLTYDNTWKGVVSVAGLSGDSL 497

Query: 506 ADFGFGVYNDHHYHIGYFLYAIAVLVKIDPAWGRKFRPHAYS--LMADFMNLSRRSNSTF 565
           ADFG   YNDHH+H GYF++  AV+  IDP W        +   L+ D  N S  ++  F
Sbjct: 498 ADFGNSYYNDHHFHYGYFVFTAAVIGHIDPDWINTGNNKEWVNFLVRDVANPSS-NDPYF 557

Query: 566 PRLRCFDLYKLHSWASGLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLT 625
           P+ R  D+Y  H WASGL E  DG+++ESTSE  N ++   L G   GD+ +    + + 
Sbjct: 558 PKHRMIDIYHGHGWASGLFESNDGKDEESTSEDYNFFFGMKLWGQVIGDSDMEDRANIIL 617

Query: 626 ALEIKAAQTWWQIREGDNLYDVDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVL 685
            +E  A   +    +G+        + N V G+ + NK     +F      E   GI +L
Sbjct: 618 GIERNALNKYMLYADGN--VQPTSMQPNYVAGITFMNKITHTTYFGTN--IEYIQGIHML 677

Query: 686 PLLPITEILFSDAGFAKELVDW-TLPSLGREGVGEGWKGFAYALQGVYDKDGVVEKIR-- 704
           P+ PI+  +    G +  L +W  L +   + V  GW+   YA   + + +   E     
Sbjct: 678 PITPISAFI---RGPSFVLAEWNALLASVIDYVDSGWRSLLYANLAIAEPEESYEYFSSS 726

BLAST of Lsi03G002200 vs. Swiss-Prot
Match: ENG1_YEAST (Endo-1,3(4)-beta-glucanase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=DSE4 PE=1 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 4.8e-33
Identity = 191/731 (26.13%), Postives = 315/731 (43.09%), Query Frame = 1

Query: 23   SSSSSSKRYKFSSPSPPLSPPPPPEMSSPMQPFWQSPPPVAALF-------PQTQSTVLP 82
            SSSSSS++ K SS S   S       +        S   +  LF       P T     P
Sbjct: 365  SSSSSSRQTKTSSSSLSTSTSSTATTTEN-----SSTTTIVNLFNAVSTDEPPTVFDRSP 424

Query: 83   DPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLI---KSSLSTVSISYPSMFLN 142
            +P +         P+ TN F+ N  V + + P +++PY +    SS    ++ + ++   
Sbjct: 425  NPMSLADGVSNDGPIQTNKFYTNLIVGSQESPAFVYPYSLWKYTSSSYGFAVQHTTVDQY 484

Query: 143  SAFGYQVF-NADWTVSENPSSVSQKPHIISSFSDLSLTLDIP-----------------S 202
            S  GY    NA++ V  NP  ++      S+F D S+T+ +                  S
Sbjct: 485  SYGGYDSSGNAEYLV--NPLGIAHVVFSASNF-DSSMTMQVDEMTLSSTRVVLSESNDSS 544

Query: 203  GNLRFFLVRGSPFMT--FEVLNNTPISIST-IHAILSFSSNS---SLTKFTVTLNNNQTW 262
              L   LV+G  F T  +    N  I  S   + I+S SS++    + K+ +TL N  TW
Sbjct: 545  NYLEIPLVQGMGFATGIYHGSLNAKIGSSVGFNTIVSESSSNLAQGILKYRITLLNGVTW 604

Query: 263  LIYSSSPINLTHSLSQI---------TSGGFSG-IIRIAVLPNP-HCETILDRFSSCYPV 322
            L Y   P +LT +   +          S    G II++AV P+    E   D+ +  Y  
Sbjct: 605  LCYVIGPDDLTSTDFSLEVSSEYEIKASASVDGLIIQLAVAPSETDYEVFYDQAAGMYVT 664

Query: 323  SGE---VNFRNPFCLEYKWEKNGW---GNLLMLAHPLHLR----LLSGYDSGAVVLDDFK 382
            + +   V+  +    E+ +   G    G+ ++ A P H      ++  Y +G  +    K
Sbjct: 665  NFKLQGVSDGSTATYEFSYTTQGESASGSTMIFALPHHESSFSDIMQDYYTGIQLASTTK 724

Query: 383  YKSIDGDLVGIVGGSWVLKPDPVSVTWHSINGVGE-EFQSEIISALVK----DVEGLKSS 442
               ++G L   +  S  L      + W S  G    E+  E +  L +    +++   S 
Sbjct: 725  -GVMNGYLTTSLQFSTSLNRQISWLPWSSQLGSNLLEYSKEQLQLLAEVANSELQVSISE 784

Query: 443  PITTTSSYFYGKSIARAARLALIAEEVNFLQVIPEVRKFL---KGAIEPWLHGTFNGNGF 502
             I+  ++Y+ GK I + + + L   E+  +Q     +  L   K A +  L         
Sbjct: 785  SISGLNTYYLGKVIDKYSYILLTVSEI--IQDEASTKSTLENIKSAFDILLQNE-QTYPL 844

Query: 503  LYDGKWGGLVTQQ--GSTNSGADFGFGVYNDHHYHIGYFLYAIAVL----VKIDPAWGRK 562
            +YD K+ GLV+    GST++  DFG   YNDHH+H GY ++A AV+     K++  W   
Sbjct: 845  IYDTKFNGLVSSGDWGSTSTQYDFGNTYYNDHHFHYGYIIHAAAVIGYVDSKLNGTWAAD 904

Query: 563  FRPHAYSLMADFMNLSRRSNSTFPRLRCFDLYKLHSWASGLTEFADGRNQESTSEAVNAY 622
             +    SL+ D  N S + +  F + R FD +  HSWA+GL E  +G+N+ES+SE  N  
Sbjct: 905  NKDWVNSLVRDVANPSEK-DEYFAQSRMFDWFNGHSWAAGLYENGNGKNEESSSEDYNFA 964

Query: 623  YSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIREGDNLYDVDFARENKVVGVLWSN 682
            Y+  L G   GD  +   G  + ++ +K A   +   + DN  + +    NKV G+L+ N
Sbjct: 965  YAMKLWGATIGDQSMELRGDLMISI-MKDAMNDYFYYQNDNTVEPEEIIGNKVSGILFDN 1024

Query: 683  KRDSGLWFAPPDWRECRLGIQVLPLLPITEILFSDAGFAKELVDWTLPSLGREGVGEGWK 685
              D   +F      E   GI +LP+ P++  + S+    +E      P +  E +  GW 
Sbjct: 1025 IIDYTTYFGTN--TEYIHGIHMLPITPVSSNIRSETFVEEEWQTKIEPII--ESIESGWT 1077

BLAST of Lsi03G002200 vs. Swiss-Prot
Match: ENG1_CANAL (Endo-1,3(4)-beta-glucanase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=ENG1 PE=1 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 4.5e-31
Identity = 158/657 (24.05%), Postives = 274/657 (41.70%), Query Frame = 1

Query: 89   PLPTNSFFQNFTVKNGDQPEYIHPYLIKSSLSTV----------------SISYPSMFLN 148
            P+ TN F+ N  + N D   Y  PY +  S ++                 SI+  +  + 
Sbjct: 460  PIGTNKFYTNLLLGNQDFMVYPLPYGLYWSKTSYYGFAVQHNNVSDRVFGSINTNNKGVA 519

Query: 149  SAFGYQVFNADWTVSENPSSVSQKPHIISSFSDLSLTLDIPSGN------LRFFLVRGSP 208
            S +     NA+   S    S       +S  ++LS  + + S +      L   LV+G  
Sbjct: 520  SYYFNPTNNAELIFSATSFSKDSMHMKVSQMAELSALVTLSSSSNDESNYLDIPLVQGMG 579

Query: 209  FMTFEVLNNTPISISTIHAILSFSSNSS------LTKFTVTLNNNQTWLIYSSSPINLTH 268
            F+T     N    ++++  +   S  +S      + K+  TL NN  WLIY + P   T 
Sbjct: 580  FVTGIYNGNLTPLLNSLFGVKDLSLETSDALLSNVLKYRATLLNNVQWLIYVTLPDKDTD 639

Query: 269  SLSQIT-------SGGFSG-IIRIAVLPNPHC-ETILDRFSSCY----PVSGEVNFRNPF 328
               ++        S    G II++A+ P  +  +   D  +  Y     VSG V+     
Sbjct: 640  FKLEVEDFYNLKGSKPVDGLIIQVAIAPEDNDNDKYYDAAAGMYVTGATVSGSVSQGTAA 699

Query: 329  CLEYKWE---KNGWGNLLMLAHPLHLRLLSGYDSGA---VVLDDFKYKSIDGDLVGIVGG 388
              ++ +    K+   N ++ A P H+  L+G    A   + +       + G L   +  
Sbjct: 700  SYKFSYTTAGKSSSNNPIVFALPHHMDSLTGSALDALTGITVTSTTKGEMTGFLTNELEF 759

Query: 389  SWVLKPDPVSVTWHSINGVGEEFQSEIISALVKDVEGLKSSPITTT-----SSYFYGKSI 448
            S  +  D   + W         +  + +  L        ++ I  T     S+YF GK +
Sbjct: 760  SETINQDVEFLPWTENMTGSLTYTKDQLELLASAANKELAANIAATVKNMNSNYFSGKVL 819

Query: 449  ARAARLALIAEEV-NFLQVIPEVRKFLKGAIEPWLHGTFNGNGFLYDGKWGGLVT---QQ 508
             + A++ L+  E+    +V  +    +K A + +          +YD K+GG+ +   Q 
Sbjct: 820  DKYAQILLVVSEIIQDEEVTKDALNAMKDAFKVFTQNK-QYYPLMYDTKFGGVTSTSAQD 879

Query: 509  GSTNSGADFGFGVYNDHHYHIGYFLYAIAVLVKIDP----AWGRKFRPHAYSLMADFMNL 568
            G  N  ADFG   YNDH +H GYF++A A++  +D      W +  +    SL+ D  N 
Sbjct: 880  GDPN--ADFGSAYYNDHDFHYGYFIHAAAIVGYVDKKLGGTWAQSNKDWVNSLVRDASNP 939

Query: 569  SRRSNSTFPRLRCFDLYKLHSWASGLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHL 628
            S   ++ FP  R FD +  HSWA+GL  F   +N ES+SE+++   +  L G   GD  +
Sbjct: 940  S-ADDTYFPVSRMFDWFSGHSWATGL--FVTYKNIESSSESLHFAAAIKLWGKVVGDQSM 999

Query: 629  ASIGSTLTALEIKAAQTWWQIREGDNLYDVDFARENKVVGVLWSNKRDSGLWF-APPDWR 685
             + G  + ++  ++   ++  +  DN  +      NKV G+ + NK D   +F  P D  
Sbjct: 1000 EARGGLMISIMARSFNMYFYYK-SDNTVEPKQILPNKVSGIFFENKVDYTTFFGTPADHP 1059

BLAST of Lsi03G002200 vs. TrEMBL
Match: E5GCT5_CUCME (Beta-glucan-binding protein OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 1399.0 bits (3620), Expect = 0.0e+00
Identity = 692/739 (93.64%), Postives = 708/739 (95.81%), Query Frame = 1

Query: 1   MLKKLRRNFKKFKTLINKSFNYSSSSSS---KRYKFSS-PSPPLSPPPPPEMSSPMQPFW 60
           MLKKL+ NFKKFKTLINK+FNYSSSSSS   KRYKFSS PSPP   PPPPEMSSP QPFW
Sbjct: 6   MLKKLKGNFKKFKTLINKTFNYSSSSSSSSSKRYKFSSSPSPPPLSPPPPEMSSPSQPFW 65

Query: 61  QSPPPVAALFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIK 120
           QSPPPVAALFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIK
Sbjct: 66  QSPPPVAALFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIK 125

Query: 121 SSLSTVSISYPSMFLNSAFGYQVFNADWTVSENPSSVSQKPHIISSFSDLSLTLDIPSGN 180
           SSLSTVSISYPSMF NSAFG+QVFNADWTVSENPSSVSQKPHIISSFSDLSLTLDIPSGN
Sbjct: 126 SSLSTVSISYPSMFSNSAFGFQVFNADWTVSENPSSVSQKPHIISSFSDLSLTLDIPSGN 185

Query: 181 LRFFLVRGSPFMTFEVLNNTPISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIYSSSPI 240
           LRFFLVRGSPFMTFEV NNT ISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIY+SSPI
Sbjct: 186 LRFFLVRGSPFMTFEVFNNTQISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIYASSPI 245

Query: 241 NLTHSLSQITSGGFSGIIRIAVLPNPHCETILDRFSSCYPVSGEVNFRNPFCLEYKWEKN 300
           NLTHSLSQITSGGF GI+RIAVLPNPHCETILDRFSSCYPVSGEVNFRNPF LEYKWEK 
Sbjct: 246 NLTHSLSQITSGGFCGIVRIAVLPNPHCETILDRFSSCYPVSGEVNFRNPFSLEYKWEKK 305

Query: 301 GWGNLLMLAHPLHLRLLSGYDSGAVVLDDFKYKSIDGDLVGIVGGSWVLKPDPVSVTWHS 360
           GWGNLLMLAHPLHLRLL+G  SGAVVLD+FKYKSIDGDLVG+VGGSWVLKPDPV VTWHS
Sbjct: 306 GWGNLLMLAHPLHLRLLAGDGSGAVVLDNFKYKSIDGDLVGVVGGSWVLKPDPVYVTWHS 365

Query: 361 INGVGEEFQSEIISALVKDVEGLKSSPITTTSSYFYGKSIARAARLALIAEEVNFLQVIP 420
           INGVGEEF SEIISALVKDVEGLKSSPITTT+SYFYGKSIARAARLALIAEEVN+LQVIP
Sbjct: 366 INGVGEEFHSEIISALVKDVEGLKSSPITTTASYFYGKSIARAARLALIAEEVNYLQVIP 425

Query: 421 EVRKFLKGAIEPWLHGTFNGNGFLYDGKWGGLVTQQGSTNSGADFGFGVYNDHHYHIGYF 480
           EVRKFLKGAIEPWL GTFNGNGFLYDGKWGGLVTQQGS +SG DFGFGVYNDHHYHIGYF
Sbjct: 426 EVRKFLKGAIEPWLLGTFNGNGFLYDGKWGGLVTQQGSHDSGGDFGFGVYNDHHYHIGYF 485

Query: 481 LYAIAVLVKIDPAWGRKFRPHAYSLMADFMNLSRRSNSTFPRLRCFDLYKLHSWASGLTE 540
           LYAIAVLVKIDPAWGRKF+PHAYSLMADFMNLSRRSNS FPRLRCFDLYKLHSWASGLTE
Sbjct: 486 LYAIAVLVKIDPAWGRKFKPHAYSLMADFMNLSRRSNSMFPRLRCFDLYKLHSWASGLTE 545

Query: 541 FADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIREGDNLY 600
           FADGRNQESTSEAVN YYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQI+EGDNLY
Sbjct: 546 FADGRNQESTSEAVNGYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIKEGDNLY 605

Query: 601 DVDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEILFSDAGFAKELV 660
           + DFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEILFSDA F KELV
Sbjct: 606 EEDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEILFSDADFVKELV 665

Query: 661 DWTLPSLGREGVGEGWKGFAYALQGVYDKDGVVEKIRNLTGFDDGNSLSNLLWWIHSRRG 720
           DWTLPSLGREGVGEGWKGFAYALQG+YDKDG +EKIRNLTGFDDGNSLSNLLWWIHS RG
Sbjct: 666 DWTLPSLGREGVGEGWKGFAYALQGIYDKDGALEKIRNLTGFDDGNSLSNLLWWIHS-RG 725

Query: 721 GGDEGEGSGGGWKHWWFSH 736
           GGDE EG GGGWKHWWFSH
Sbjct: 726 GGDEVEGCGGGWKHWWFSH 743

BLAST of Lsi03G002200 vs. TrEMBL
Match: A0A0A0KSJ9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G643880 PE=4 SV=1)

HSP 1 Score: 1381.3 bits (3574), Expect = 0.0e+00
Identity = 684/738 (92.68%), Postives = 701/738 (94.99%), Query Frame = 1

Query: 1   MLKKLRRNFKKFKTLINKSFNYSSSSSS--KRYKFSS-PSPPLSPPPPPEMSSPMQPFWQ 60
           MLKKL+ NFKK +TLINKSFN SSSSSS  KRYKFSS PSPP   PPP EMSSP QPFWQ
Sbjct: 1   MLKKLKGNFKKIRTLINKSFNNSSSSSSSSKRYKFSSSPSPPPLSPPPLEMSSPSQPFWQ 60

Query: 61  SPPPVAALFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKS 120
           SPPPVAALFPQT+S+VLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKS
Sbjct: 61  SPPPVAALFPQTESSVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKS 120

Query: 121 SLSTVSISYPSMFLNSAFGYQVFNADWTVSENPSSVSQKPHIISSFSDLSLTLDIPSGNL 180
           SLSTVS+SYPSM  NSAFGYQVFNADWTVSENP SVSQKPHIISSFSDLSLTLDIPSGNL
Sbjct: 121 SLSTVSVSYPSMLSNSAFGYQVFNADWTVSENPPSVSQKPHIISSFSDLSLTLDIPSGNL 180

Query: 181 RFFLVRGSPFMTFEVLNNTPISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIYSSSPIN 240
           RFFLVRGSPFMTFEV NNT ISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIY+SSPIN
Sbjct: 181 RFFLVRGSPFMTFEVFNNTQISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIYASSPIN 240

Query: 241 LTHSLSQITSGGFSGIIRIAVLPNPHCETILDRFSSCYPVSGEVNFRNPFCLEYKWEKNG 300
           LTHSLSQITSGGF+GI+RIAVLPNPHCETILDRFSSCYPVSGEVNFRNPF LEYKWEK G
Sbjct: 241 LTHSLSQITSGGFTGIVRIAVLPNPHCETILDRFSSCYPVSGEVNFRNPFSLEYKWEKKG 300

Query: 301 WGNLLMLAHPLHLRLLSGYDSGAVVLDDFKYKSIDGDLVGIVGGSWVLKPDPVSVTWHSI 360
           WGNLLMLAHPLHLRLLSG  S AVVLDDFKYKSIDGDLVG+VGGSW LKPDPV +TWHSI
Sbjct: 301 WGNLLMLAHPLHLRLLSGDGSSAVVLDDFKYKSIDGDLVGVVGGSWALKPDPVYLTWHSI 360

Query: 361 NGVGEEFQSEIISALVKDVEGLKSSPITTTSSYFYGKSIARAARLALIAEEVNFLQVIPE 420
           NGVGEEF SEIISALVKDVEGLKSSPITTTSSYFYGKSIARAARLALIAEEVN+LQVIPE
Sbjct: 361 NGVGEEFHSEIISALVKDVEGLKSSPITTTSSYFYGKSIARAARLALIAEEVNYLQVIPE 420

Query: 421 VRKFLKGAIEPWLHGTFNGNGFLYDGKWGGLVTQQGSTNSGADFGFGVYNDHHYHIGYFL 480
           VRKFLKGAIEPWL GTFNGNGFLYDGKWGGLVTQQGS +SG DFGFGVYNDHHYHIGYFL
Sbjct: 421 VRKFLKGAIEPWLLGTFNGNGFLYDGKWGGLVTQQGSHDSGGDFGFGVYNDHHYHIGYFL 480

Query: 481 YAIAVLVKIDPAWGRKFRPHAYSLMADFMNLSRRSNSTFPRLRCFDLYKLHSWASGLTEF 540
           YAIAVLVKIDPAWGRKF+PHAYSLMADFMNLSRRSNS FPRLRCFDLYKLHSWASGLTEF
Sbjct: 481 YAIAVLVKIDPAWGRKFKPHAYSLMADFMNLSRRSNSMFPRLRCFDLYKLHSWASGLTEF 540

Query: 541 ADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIREGDNLYD 600
           ADGRNQESTSEAVN YYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQI+EGDNLY+
Sbjct: 541 ADGRNQESTSEAVNGYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIKEGDNLYE 600

Query: 601 VDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEILFSDAGFAKELVD 660
            DFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLP+LPITEILFSDA F KELVD
Sbjct: 601 EDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPILPITEILFSDADFVKELVD 660

Query: 661 WTLPSLGREGVGEGWKGFAYALQGVYDKDGVVEKIRNLTGFDDGNSLSNLLWWIHSRRGG 720
           WTLPSLGREGVGEGWKGFAYALQGVYDKDG +EKIRNLTGFDDGNSLSNLLWWIHS RGG
Sbjct: 661 WTLPSLGREGVGEGWKGFAYALQGVYDKDGALEKIRNLTGFDDGNSLSNLLWWIHS-RGG 720

Query: 721 GDEGEGSGGGWKHWWFSH 736
           GDE E  GGGWKHWWFSH
Sbjct: 721 GDEVEACGGGWKHWWFSH 737

BLAST of Lsi03G002200 vs. TrEMBL
Match: W9R5F5_9ROSA (Endo-1,3(4)-beta-glucanase 1 OS=Morus notabilis GN=L484_009128 PE=4 SV=1)

HSP 1 Score: 1069.3 bits (2764), Expect = 2.1e-309
Identity = 533/748 (71.26%), Postives = 612/748 (81.82%), Query Frame = 1

Query: 1   MLKKLRRNFKKFKTLINKSFN-------YSSSSSSKRYKFSSPSPP--LSPPPPPEMSSP 60
           MLKKLRR   + KTL+ K+F        +S+ S S   +  SPS P  +  P PP+MS  
Sbjct: 1   MLKKLRR---RVKTLVTKTFKKPRRRPEFSTPSPSTPVEVPSPSTPEEVVEPEPPKMS-- 60

Query: 61  MQPFWQSPPPVAALFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIH 120
           +   +Q       LFP+ QSTV+ DPS FF+P LLS+PLPTNSFFQNF +KNGDQPEYIH
Sbjct: 61  LSSSFQKRSNAPFLFPKAQSTVVSDPSLFFSPDLLSTPLPTNSFFQNFVLKNGDQPEYIH 120

Query: 121 PYLIKSSLSTVSISYPSMFLNSAFGYQVFNADWTVS--ENPSSVSQKPHIISSFSDLSLT 180
           PYL+KSS S++S+ YPS   NSAF YQ+FNAD TVS  E  +  SQKPH+ISSF+DLSLT
Sbjct: 121 PYLVKSSNSSLSVCYPSRLSNSAFLYQIFNADLTVSAAEKTAPDSQKPHLISSFNDLSLT 180

Query: 181 LDIPSGNLRFFLVRGSPFMTFEVLNNTPISISTIHAILSFSSNSSLTKFTVTLNNNQTWL 240
           LDIPS NLRFFLVRGSPF+T      T ISISTIHAILSFSSN+S TK+TV LNNNQTWL
Sbjct: 181 LDIPSSNLRFFLVRGSPFLTCTASAKTEISISTIHAILSFSSNNSQTKYTVKLNNNQTWL 240

Query: 241 IYSSSPINLTHSLSQITSGGFSGIIRIAVLP--NPHCETILDRFSSCYPVSGEVNFRNPF 300
           IY+SSPI+ +HSLS +TS GFSGI+RIA+LP  NP  E ILDRFSSCYPVSGEV+F  PF
Sbjct: 241 IYTSSPISFSHSLSLLTSNGFSGILRIAILPDSNPEFEAILDRFSSCYPVSGEVSFTKPF 300

Query: 301 CLEYKWEKNGWGNLLMLAHPLHLRLLSGYDSGAVVLDDFKYKSIDGDLVGIVGGSWVLKP 360
           CLEYKWEK GWG+LLMLAHPLHL+LL G D G  VL++FKYKSIDGDLVG+VG SW LKP
Sbjct: 301 CLEYKWEKKGWGDLLMLAHPLHLKLLKGDDRGVTVLEEFKYKSIDGDLVGVVGDSWTLKP 360

Query: 361 DPVSVTWHSINGVGEEFQSEIISALVKDVEGLKSSPITTTSSYFYGKSIARAARLALIAE 420
           DPVSVTWHSI GV EE   EI++AL KDVE L ++ ITT +SYFYGK IARAAR ALIAE
Sbjct: 361 DPVSVTWHSIEGVKEEAYEEIVAALQKDVEALDAAAITTNASYFYGKLIARAARFALIAE 420

Query: 421 EVNFLQVIPEVRKFLKGAIEPWLHGTFNGNGFLYDGKWGGLVTQQGSTNSGADFGFGVYN 480
           EV F  VIP++RKFLK AIEPWL GTFNGNGFLY+ KWGG++T+QG  + GADFGFG+YN
Sbjct: 421 EVRFFDVIPKIRKFLKEAIEPWLDGTFNGNGFLYEEKWGGIITKQGQGDGGADFGFGIYN 480

Query: 481 DHHYHIGYFLYAIAVLVKIDPAWGRKFRPHAYSLMADFMNLSRRSNSTFPRLRCFDLYKL 540
           DHH+H+G+FLYAIAVL KIDPAWGRK++P AYS+MADFMNLSRR+ S +PRLRCFDLYKL
Sbjct: 481 DHHFHLGHFLYAIAVLAKIDPAWGRKYKPQAYSMMADFMNLSRRAKSNYPRLRCFDLYKL 540

Query: 541 HSWASGLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWW 600
           HSWA GLTEFADGRNQES+SEAVNAYYSAAL+GLAYGDTHL +IGS LTALEI+AAQ WW
Sbjct: 541 HSWAGGLTEFADGRNQESSSEAVNAYYSAALMGLAYGDTHLVAIGSLLTALEIQAAQMWW 600

Query: 601 QIREGDNLYDVDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEILFS 660
            ++EGD LYD DF REN+VVGVLWSNKRDSGLWFAP  WRECRLGIQVLPLLPITE+LFS
Sbjct: 601 HVKEGDKLYDEDFTRENRVVGVLWSNKRDSGLWFAPAAWRECRLGIQVLPLLPITEVLFS 660

Query: 661 DAGFAKELVDWTLPSLGREGVGEGWKGFAYALQGVYDKDGVVEKIRNLTGFDDGNSLSNL 720
           D GF +ELV+WTLP+L REGVGEGWKGF YAL+G+YDK+  ++KI++L GFDDGNSL+NL
Sbjct: 661 DVGFTRELVNWTLPALQREGVGEGWKGFVYALEGIYDKETALQKIKSLNGFDDGNSLTNL 720

Query: 721 LWWIHSRRGGGDEGEGSGGGWKHWWFSH 736
           LWWIHSR     E E  GGG KH WF+H
Sbjct: 721 LWWIHSR---DQEKEEFGGGGKHCWFAH 740

BLAST of Lsi03G002200 vs. TrEMBL
Match: V4STK6_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10011145mg PE=4 SV=1)

HSP 1 Score: 1063.1 bits (2748), Expect = 1.5e-307
Identity = 535/746 (71.72%), Postives = 614/746 (82.31%), Query Frame = 1

Query: 1   MLKKLRRNFKKFKTLINKSFN-----YSSSSSSKRYKFSSPSPPLSPPPPPEMSSPMQPF 60
           MLKKL+R   + KT+I K F      +   ++      +SPSPP SP   PE    M+P 
Sbjct: 1   MLKKLKR---RVKTIITKPFKKPRKPHKPPNTKSPSPSASPSPPDSPKLEPEPPPTMEPQ 60

Query: 61  WQSP-PPVAALFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYL 120
                P    LFP+ QSTVLPDPS FFAP LLSSPLPTNSFFQNFT+K GDQPEYIHPYL
Sbjct: 61  HSKKLPTFPFLFPKAQSTVLPDPSLFFAPSLLSSPLPTNSFFQNFTLKAGDQPEYIHPYL 120

Query: 121 IKSSLSTVSISYPSMFLNSAFGYQVFNADWTVS--ENPSSVSQKPHIISSFSDLSLTLDI 180
           IKSSLS++S+SYPS F N +F YQVFNAD  +S   N    S+K HIISS+SDLS+TLD+
Sbjct: 121 IKSSLSSLSVSYPSRFHNPSFIYQVFNADLIISASNNTEPSSRKSHIISSYSDLSVTLDM 180

Query: 181 PSGNLRFFLVRGSPFMTFEVLNNTPISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIYS 240
           PS N RFFLVRGSPF+T  ++NNTP+SISTIHAILS  +N+SLTK+T+ LNNNQTW +YS
Sbjct: 181 PSANFRFFLVRGSPFLTCSIVNNTPVSISTIHAILSCDANNSLTKYTIKLNNNQTWFLYS 240

Query: 241 SSPINLTHS-LSQITSGGFSGIIRIAVLPN--PHCETILDRFSSCYPVSGEVNFRNPFCL 300
           SSPI+L+ + +S +TS GF+GIIRIAVLP+  P  ++ILDRFSSCYPVSG+  F  PFCL
Sbjct: 241 SSPISLSQNDVSLLTSDGFTGIIRIAVLPDFDPQYQSILDRFSSCYPVSGDAVFTKPFCL 300

Query: 301 EYKWEKNGWGNLLMLAHPLHLRLLSGYDSGAVVLDDFKYKSIDGDLVGIVGGSWVLKPDP 360
           EYK++K GWG+LLMLAHPLHL +LS  DS   VL+DFKYKSIDGDLVG+VG SWVLKP  
Sbjct: 301 EYKFQKKGWGDLLMLAHPLHLNMLSIDDSDVSVLEDFKYKSIDGDLVGVVGDSWVLKPHS 360

Query: 361 VSVTWHSINGVGEEFQSEIISALVKDVEGLKSSPITTTSSYFYGKSIARAARLALIAEEV 420
           VS+TWHSING  EE   EIISAL KDV+ L S+PITT SSYFYGK IARAARLALIAEEV
Sbjct: 361 VSITWHSINGNREESYDEIISALSKDVKALNSTPITTNSSYFYGKLIARAARLALIAEEV 420

Query: 421 NFLQVIPEVRKFLKGAIEPWLHGTFNGNGFLYDGKWGGLVTQQGSTNSGADFGFGVYNDH 480
           +F  VIP +RK+L+  IEPWL GTFNGNGFLYDGKWGG+VT+QGS +SGADFGFGVYNDH
Sbjct: 421 SFPDVIPAIRKYLRETIEPWLDGTFNGNGFLYDGKWGGIVTKQGSLDSGADFGFGVYNDH 480

Query: 481 HYHIGYFLYAIAVLVKIDPAWGRKFRPHAYSLMADFMNLSRRSNSTFPRLRCFDLYKLHS 540
           HYH+GYFLYAIAVLVKIDP WGRK+RP AYSLMADFMNL RR NS +PRLRCFDLYKLHS
Sbjct: 481 HYHLGYFLYAIAVLVKIDPVWGRKYRPQAYSLMADFMNLGRRENSNYPRLRCFDLYKLHS 540

Query: 541 WASGLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQI 600
           WA GLTEFADGRNQESTSEAVNAYYSAAL+GLAYGDTHL +IGSTLTA+EI+AAQTWW I
Sbjct: 541 WAGGLTEFADGRNQESTSEAVNAYYSAALMGLAYGDTHLVAIGSTLTAMEIQAAQTWWHI 600

Query: 601 REGDNLYDVDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEILFSDA 660
           RE D LYD DF REN+VVGVLW+NKRDSGLWFAPPDWRECRLGIQ+LPL PI+E+LFS+ 
Sbjct: 601 REEDTLYDNDFTRENRVVGVLWANKRDSGLWFAPPDWRECRLGIQLLPLSPISEVLFSEV 660

Query: 661 GFAKELVDWTLPSLGREGVGEGWKGFAYALQGVYDKDGVVEKIRNLTGFDDGNSLSNLLW 720
           GF ++LV+WTLP+LGREGVGEGWKGF Y+L+G+YDKD  ++KIRNL G+DDGNSL+NLLW
Sbjct: 661 GFVRQLVNWTLPALGREGVGEGWKGFVYSLEGIYDKDSALQKIRNLNGYDDGNSLTNLLW 720

Query: 721 WIHSRRGGGDEGEGSGGGWKHWWFSH 736
           WIHS RG  +E E SGGG K  WF H
Sbjct: 721 WIHS-RGEVEEEECSGGG-KFCWFGH 741

BLAST of Lsi03G002200 vs. TrEMBL
Match: A0A061EHI7_THECC (Glycosyl hydrolase family 81 protein OS=Theobroma cacao GN=TCM_019610 PE=4 SV=1)

HSP 1 Score: 1057.0 bits (2732), Expect = 1.1e-305
Identity = 537/743 (72.27%), Postives = 607/743 (81.70%), Query Frame = 1

Query: 1   MLKKLRRNFKKFKTLINKSFNYSSSSSSKRYKFSSPSPPLSPPPPPEMSSPMQPFWQSPP 60
           MLKKL R   K KTLI K F        KR K     PP SP P P  S PM P    P 
Sbjct: 1   MLKKLGR---KVKTLITKPF--------KRPK-KPCKPPKSPSPSPSPSPPMSP----PK 60

Query: 61  PVAA---LFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKS 120
           P      LFP  QS+VLPDPS FF+P LLS+PLPTNSFFQNFT+KNGDQPEYIHPYLIKS
Sbjct: 61  PTKYCPFLFPGVQSSVLPDPSVFFSPKLLSTPLPTNSFFQNFTLKNGDQPEYIHPYLIKS 120

Query: 121 SLSTVSISYPSMFLNSAFGYQVFNADWTV--SENPSSVSQKPHIISSFSDLSLTLDIPSG 180
           + S++SISYPS F NS+F YQ+F +D T+  ++  +  SQK HIISS+SDLSLTLD P  
Sbjct: 121 ADSSLSISYPSHFHNSSFLYQIFISDLTIFATDRTNPTSQKNHIISSYSDLSLTLDFPCS 180

Query: 181 NLRFFLVRGSPFMTFEVLNNTPISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIYSSSP 240
           NLRF+LVRGSPF+T  V  NTPISISTIHAILSFSSN+SLTK T+ LNNNQTWLIY+SS 
Sbjct: 181 NLRFYLVRGSPFLTCFVTGNTPISISTIHAILSFSSNTSLTKHTIKLNNNQTWLIYTSSA 240

Query: 241 INLTHSLSQITSGGFSGIIRIAVLPN--PHCETILDRFSSCYPVSGEVNFRNPFCLEYKW 300
           I+L HSLS ITSGGFSGIIRIAVLP+  P  E+ILD++SSCYP+SG+     PFCLEYKW
Sbjct: 241 ISLNHSLSLITSGGFSGIIRIAVLPDSDPTYESILDQYSSCYPISGDAVLTKPFCLEYKW 300

Query: 301 EKNGWGNLLMLAHPLHLRLLSGYDSGAVVLDDFKYKSIDGDLVGIVGGSWVLKPDPVSVT 360
           EK GWG+LLMLAHPLHL +LS  DS   VL++FKYKSIDG+LVGIVG SWVLKP  VSVT
Sbjct: 301 EKKGWGDLLMLAHPLHLNILSRDDSEVTVLENFKYKSIDGELVGIVGDSWVLKPHQVSVT 360

Query: 361 WHSINGVGEEFQSEIISALVKDVEGLKSSPITTTSSYFYGKSIARAARLALIAEEVNFLQ 420
           WHSI GV EE   EI++AL KDVE L SS ITTTSSYFYGK IARAARLALIAEEV FL 
Sbjct: 361 WHSIKGVKEESYHEIVTALSKDVEALDSSAITTTSSYFYGKLIARAARLALIAEEVCFLD 420

Query: 421 VIPEVRKFLKGAIEPWLHGTFNGNGFLYDGKWGGLVTQQGSTNSGADFGFGVYNDHHYHI 480
           VIP +RKFLK  IEPWL GTFNGNGFLYD KWGG+VT+QGST+SGADFGFG+YNDHHYHI
Sbjct: 421 VIPAIRKFLKDTIEPWLDGTFNGNGFLYDSKWGGIVTKQGSTDSGADFGFGIYNDHHYHI 480

Query: 481 GYFLYAIAVLVKIDPAWGRKFRPHAYSLMADFMNLSRRSNSTFPRLRCFDLYKLHSWASG 540
           GYFLYAIAVL KIDPAWGR+++P AY+L+ADFMNL RRSNS +PRLRCFDLYKLHSWA G
Sbjct: 481 GYFLYAIAVLAKIDPAWGRRYKPQAYTLVADFMNLGRRSNSNYPRLRCFDLYKLHSWAGG 540

Query: 541 LTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIREGD 600
           LTEFADGRNQESTSEAVNAYYSAAL+GLAYGDTHL + GSTL A+EI+AAQTWW ++EGD
Sbjct: 541 LTEFADGRNQESTSEAVNAYYSAALMGLAYGDTHLVATGSTLAAMEIQAAQTWWHVKEGD 600

Query: 601 NLYDVDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEILFSDAGFAK 660
           NLY+ +F  EN+VVGVLW+NKRDSGLWFAP DWRECRLGIQ+LP+LPI+E+LFSD  F +
Sbjct: 601 NLYEEEFTTENRVVGVLWANKRDSGLWFAPADWRECRLGIQLLPILPISEVLFSDISFTR 660

Query: 661 ELVDWTLPSLGREGVGEGWKGFAYALQGVYDKDGVVEKIRNLTGFDDGNSLSNLLWWIHS 720
           ELV+WTLP+LGREGVGEGWKGF YAL+ +YDK+  +EKIRNL G+DDGNSL+NLLWW+HS
Sbjct: 661 ELVNWTLPALGREGVGEGWKGFVYALEAIYDKESALEKIRNLNGYDDGNSLTNLLWWVHS 720

Query: 721 RRGGGDEGEGS-GGGWKHWWFSH 736
           R   GDE E   G   K+ WFSH
Sbjct: 721 R---GDEHEDKFGTEGKYCWFSH 724

BLAST of Lsi03G002200 vs. TAIR10
Match: AT5G15870.1 (AT5G15870.1 glycosyl hydrolase family 81 protein)

HSP 1 Score: 963.0 bits (2488), Expect = 1.1e-280
Identity = 494/757 (65.26%), Postives = 578/757 (76.35%), Query Frame = 1

Query: 1   MLKKLRRNFKKFKTLINKSFNYSSSSSSKRYKFSSPSPP----LSPPPPPEMSSPMQPFW 60
           MLKK+RR   K K LI K F        K+ K   PSPP    L P P P  S  M    
Sbjct: 1   MLKKVRR---KVKVLITKPF--------KKPKNRPPSPPPPLPLPPSPSPPPSQQMSSSR 60

Query: 61  QSPPPVAALFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIK 120
           Q   P   LFP++ S+VLPDPS FF+  LLSSPLPTNSFFQNFT+ NGDQ EY HPY+IK
Sbjct: 61  QKNTPF--LFPRSDSSVLPDPSRFFSHDLLSSPLPTNSFFQNFTLNNGDQAEYFHPYIIK 120

Query: 121 SSLSTVSISYPSMFLNSAFGYQVFNADWTV--SENPSSVSQKPHIISSFSDLSLTLDIPS 180
            S S++SISYPS+  NSAF Y+ FNAD T+  S+ P   S+K H+ISSFSDL +TLD PS
Sbjct: 121 PSTSSLSISYPSLSHNSAFIYEAFNADITITGSDGPDPHSRKSHLISSFSDLGVTLDFPS 180

Query: 181 GNLRFFLVRGSPFMTFEVLNNTPISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIYSSS 240
            NLRFFLVRGSPF+TF V  N+ I+ISTIHA+LS S N+S TK+TV LNNNQTWLIY+SS
Sbjct: 181 SNLRFFLVRGSPFITFSV--NSSITISTIHAVLSLSGNTSSTKYTVKLNNNQTWLIYASS 240

Query: 241 PINLTHS-LSQITSG-GFSGIIRIAVLPNP--HCETILDRFSSCYPVSGEVNFRNPFCLE 300
           PINLT   +S I  G GFSGIIRI VLPNP  + ETILD FS  YPVSG+ +F  PF LE
Sbjct: 241 PINLTKDGVSSINCGDGFSGIIRIVVLPNPNPYFETILDGFSCSYPVSGDADFTKPFALE 300

Query: 301 YKWEKNGWGNLLMLAHPLHLRLLSGYDSGAVVLDDFKYKSIDGDLVGIVGGSWVLKPDPV 360
           YKWEK G+G+LLMLAHPLHL+LLS  D    VLD+FKY SIDGDLVG++G SWVLKPDPV
Sbjct: 301 YKWEKRGYGDLLMLAHPLHLKLLSTNDCSITVLDNFKYNSIDGDLVGVIGDSWVLKPDPV 360

Query: 361 SVTWHSINGVGEEFQSEIISALVKDVEGLKSSPITTTSSYFYGKSIARAARLALIAEEVN 420
           SVTWHSI GV E+   EIISAL+KDV  L SS   T SSYFY K IARAARLALIAEEV 
Sbjct: 361 SVTWHSIKGVQEDSHQEIISALIKDVNALDSSAEVTNSSYFYAKLIARAARLALIAEEVC 420

Query: 421 FLQVIPEVRKFLKGAIEPWLHGTFNGNGFLYDGKWGGLVTQQGSTNSGADFGFGVYNDHH 480
           +L VIP++R +LK  IEPWL+G+F  NGFLYD KWGG++T+ GS +SGADFGFG+YNDHH
Sbjct: 421 YLDVIPKIRTYLKNMIEPWLNGSFGPNGFLYDPKWGGVITKLGSRDSGADFGFGIYNDHH 480

Query: 481 YHIGYFLYAIAVLVKIDPAWGRKFRPHAYSLMADFMNLSR---RSNSTFPRLRCFDLYKL 540
           YH+GYF+YAIAVL KIDP WG+++RP AY+LMAD++ L +   +SNS +PRLRCFDL+KL
Sbjct: 481 YHLGYFVYAIAVLAKIDPLWGKRYRPQAYTLMADYLTLGKKGAKSNSNYPRLRCFDLFKL 540

Query: 541 HSWASGLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWW 600
           HSWA GLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHL +  S +  LEI AA+ WW
Sbjct: 541 HSWAGGLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLVAAASMVLTLEIHAAKMWW 600

Query: 601 QIREGDNLYDVDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEILFS 660
           Q++E D +Y  DF  EN+VVGVLWS KRDSGLWFAP +W+ECRLGIQ+LPLLP++E+LFS
Sbjct: 601 QVKEDDAIYPQDFTSENRVVGVLWSTKRDSGLWFAPKEWKECRLGIQLLPLLPVSEVLFS 660

Query: 661 DAGFAKELVDWTLPSLGREGVGEGWKGFAYALQGVYDKDGVVEKIRNLTGFDDGNSLSNL 720
           D  F K+LV+WT+P+L R+ VGEGWKGF YAL+ +YDKDG +EKI+ L GFDDGNSLSNL
Sbjct: 661 DVTFVKQLVNWTMPALARDSVGEGWKGFVYALESMYDKDGAMEKIKGLNGFDDGNSLSNL 720

Query: 721 LWWIHSRRGGGDEG---------EGSGGGWKHWWFSH 736
           LWW+HSR    D+           G GGG K+  F H
Sbjct: 721 LWWVHSRNNDDDDDYEEDDEGGYGGHGGGGKYCSFGH 742

BLAST of Lsi03G002200 vs. TAIR10
Match: AT1G18310.1 (AT1G18310.1 glycosyl hydrolase family 81 protein)

HSP 1 Score: 838.2 bits (2164), Expect = 3.9e-243
Identity = 427/680 (62.79%), Postives = 496/680 (72.94%), Query Frame = 1

Query: 65  LFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKSSLSTVSI 124
           LFP+T+S+VLPDPS FF+P LLS+PLPTNSFFQNFT+KNGDQ EY HPYLIKS  S++ I
Sbjct: 9   LFPETKSSVLPDPSRFFSPDLLSNPLPTNSFFQNFTLKNGDQAEYFHPYLIKSVDSSLCI 68

Query: 125 SYPSMFLNSAFGYQVFNADWTVS--ENPSSVSQKPHIISSFSDLSLTLDIPSGNLRFFLV 184
           SYPS+F NS F Y+VF AD  +S    P   S+K HIISSFSDL +TLD PS NLRFFLV
Sbjct: 69  SYPSLFHNSDFFYEVFTADIIISGSNGPGPRSRKTHIISSFSDLVVTLDFPSSNLRFFLV 128

Query: 185 RGSPFMTFEVLNNTPISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIYSSSPINLT-HS 244
           RGSP                          SS TK++  L NNQ WLIY+SSPI+LT H 
Sbjct: 129 RGSP--------------------------SSSTKWSDKLTNNQRWLIYASSPIDLTKHG 188

Query: 245 LSQI-TSGGFSGIIRIAVLP--NPHCETILDRFSSCYPVSGEVNFRNPFCLEYKWEKNGW 304
            S I   GGF+GI+RI VLP  NP  E+ LDRFSSCYPVSG+ +F  PF L+Y WEK G 
Sbjct: 189 DSSIHCRGGFTGIVRITVLPGSNPGYESTLDRFSSCYPVSGDADFTKPFTLKYMWEKRGS 248

Query: 305 GNLLMLAHPLHLRLLSG--------------------YDSGAVVLDDFKYKSIDGDLVGI 364
           G+LLMLAHPLHL+LL+                      +S   VLD F+YKSIDGDLVG+
Sbjct: 249 GDLLMLAHPLHLKLLAKDASSLFQQSTLLLVCPDRGCTNSSVTVLDHFRYKSIDGDLVGV 308

Query: 365 VGGSWVLKPDPVSVTWHSINGVGEEFQSEIISALVKDVEGLKSSPITTTSSYFYGKSIAR 424
           VG SWVLKPD VSVTWHS+ GV ++   EIISAL KDV GL SS   T+SSYFYGK IAR
Sbjct: 309 VGDSWVLKPDSVSVTWHSMKGVKKDSYKEIISALGKDVNGLNSSAEVTSSSYFYGKLIAR 368

Query: 425 AARLALIAEEVNFLQVIPEVRKFLKGAIEPWLHGTFNGNGFLYDGKWGGLVTQQGSTNSG 484
           AAR ALIAEEV +L VIP++  +LK  IEPWL G+F  NGFLYD KWGGL+T+QGS +S 
Sbjct: 369 AARFALIAEEVCYLDVIPKIVTYLKNMIEPWLDGSFKPNGFLYDPKWGGLITKQGSKDSQ 428

Query: 485 ADFGFGVYNDHHYHIGYFLYAIAVLVKIDPAWGRKFRPHAYSLMADFMNLSRR----SNS 544
           ADFGFG+YNDHHYHIGYFLYAIAVL K DP WG ++R  AYSL+ADFM   R+    SNS
Sbjct: 429 ADFGFGIYNDHHYHIGYFLYAIAVLAKFDPLWGERYRAQAYSLLADFMTFGRKDDNNSNS 488

Query: 545 TFPRLRCFDLYKLHSWASGLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGST 604
           ++PRLR FDL+KLHSWA GLTEF DGRNQESTSEAVNAYYSAALLGLAYGD HL    ST
Sbjct: 489 SYPRLRNFDLFKLHSWAGGLTEFWDGRNQESTSEAVNAYYSAALLGLAYGDKHLVETAST 548

Query: 605 LTALEIKAAQTWWQIREGDNLYDVDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQ 664
           +  LEI AA+ WWQ+++G+ LY  DF  EN+VVGVLWS KRDS LWF P +W+ECRLGIQ
Sbjct: 549 IMTLEIHAAKMWWQVKKGEALYPKDFTAENRVVGVLWSTKRDSSLWFGPKEWKECRLGIQ 608

Query: 665 VLPLLPITEILFSDAGFAKELVDWTLPSLGREGVGEGWKGFAYALQGVYDKDGVVEKIRN 715
           +LP+LP              LV+WTLP+L R GVGEGWKGF YAL+ +YDKDG ++KI+ 
Sbjct: 609 LLPILP--------------LVNWTLPALQRNGVGEGWKGFLYALESLYDKDGAIKKIKR 648

BLAST of Lsi03G002200 vs. NCBI nr
Match: gi|659119012|ref|XP_008459427.1| (PREDICTED: putative endo-1,3(4)-beta-glucanase 2 [Cucumis melo])

HSP 1 Score: 1399.0 bits (3620), Expect = 0.0e+00
Identity = 692/739 (93.64%), Postives = 708/739 (95.81%), Query Frame = 1

Query: 1   MLKKLRRNFKKFKTLINKSFNYSSSSSS---KRYKFSS-PSPPLSPPPPPEMSSPMQPFW 60
           MLKKL+ NFKKFKTLINK+FNYSSSSSS   KRYKFSS PSPP   PPPPEMSSP QPFW
Sbjct: 6   MLKKLKGNFKKFKTLINKTFNYSSSSSSSSSKRYKFSSSPSPPPLSPPPPEMSSPSQPFW 65

Query: 61  QSPPPVAALFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIK 120
           QSPPPVAALFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIK
Sbjct: 66  QSPPPVAALFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIK 125

Query: 121 SSLSTVSISYPSMFLNSAFGYQVFNADWTVSENPSSVSQKPHIISSFSDLSLTLDIPSGN 180
           SSLSTVSISYPSMF NSAFG+QVFNADWTVSENPSSVSQKPHIISSFSDLSLTLDIPSGN
Sbjct: 126 SSLSTVSISYPSMFSNSAFGFQVFNADWTVSENPSSVSQKPHIISSFSDLSLTLDIPSGN 185

Query: 181 LRFFLVRGSPFMTFEVLNNTPISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIYSSSPI 240
           LRFFLVRGSPFMTFEV NNT ISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIY+SSPI
Sbjct: 186 LRFFLVRGSPFMTFEVFNNTQISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIYASSPI 245

Query: 241 NLTHSLSQITSGGFSGIIRIAVLPNPHCETILDRFSSCYPVSGEVNFRNPFCLEYKWEKN 300
           NLTHSLSQITSGGF GI+RIAVLPNPHCETILDRFSSCYPVSGEVNFRNPF LEYKWEK 
Sbjct: 246 NLTHSLSQITSGGFCGIVRIAVLPNPHCETILDRFSSCYPVSGEVNFRNPFSLEYKWEKK 305

Query: 301 GWGNLLMLAHPLHLRLLSGYDSGAVVLDDFKYKSIDGDLVGIVGGSWVLKPDPVSVTWHS 360
           GWGNLLMLAHPLHLRLL+G  SGAVVLD+FKYKSIDGDLVG+VGGSWVLKPDPV VTWHS
Sbjct: 306 GWGNLLMLAHPLHLRLLAGDGSGAVVLDNFKYKSIDGDLVGVVGGSWVLKPDPVYVTWHS 365

Query: 361 INGVGEEFQSEIISALVKDVEGLKSSPITTTSSYFYGKSIARAARLALIAEEVNFLQVIP 420
           INGVGEEF SEIISALVKDVEGLKSSPITTT+SYFYGKSIARAARLALIAEEVN+LQVIP
Sbjct: 366 INGVGEEFHSEIISALVKDVEGLKSSPITTTASYFYGKSIARAARLALIAEEVNYLQVIP 425

Query: 421 EVRKFLKGAIEPWLHGTFNGNGFLYDGKWGGLVTQQGSTNSGADFGFGVYNDHHYHIGYF 480
           EVRKFLKGAIEPWL GTFNGNGFLYDGKWGGLVTQQGS +SG DFGFGVYNDHHYHIGYF
Sbjct: 426 EVRKFLKGAIEPWLLGTFNGNGFLYDGKWGGLVTQQGSHDSGGDFGFGVYNDHHYHIGYF 485

Query: 481 LYAIAVLVKIDPAWGRKFRPHAYSLMADFMNLSRRSNSTFPRLRCFDLYKLHSWASGLTE 540
           LYAIAVLVKIDPAWGRKF+PHAYSLMADFMNLSRRSNS FPRLRCFDLYKLHSWASGLTE
Sbjct: 486 LYAIAVLVKIDPAWGRKFKPHAYSLMADFMNLSRRSNSMFPRLRCFDLYKLHSWASGLTE 545

Query: 541 FADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIREGDNLY 600
           FADGRNQESTSEAVN YYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQI+EGDNLY
Sbjct: 546 FADGRNQESTSEAVNGYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIKEGDNLY 605

Query: 601 DVDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEILFSDAGFAKELV 660
           + DFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEILFSDA F KELV
Sbjct: 606 EEDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEILFSDADFVKELV 665

Query: 661 DWTLPSLGREGVGEGWKGFAYALQGVYDKDGVVEKIRNLTGFDDGNSLSNLLWWIHSRRG 720
           DWTLPSLGREGVGEGWKGFAYALQG+YDKDG +EKIRNLTGFDDGNSLSNLLWWIHS RG
Sbjct: 666 DWTLPSLGREGVGEGWKGFAYALQGIYDKDGALEKIRNLTGFDDGNSLSNLLWWIHS-RG 725

Query: 721 GGDEGEGSGGGWKHWWFSH 736
           GGDE EG GGGWKHWWFSH
Sbjct: 726 GGDEVEGCGGGWKHWWFSH 743

BLAST of Lsi03G002200 vs. NCBI nr
Match: gi|449447468|ref|XP_004141490.1| (PREDICTED: endo-1,3(4)-beta-glucanase 1-like [Cucumis sativus])

HSP 1 Score: 1381.3 bits (3574), Expect = 0.0e+00
Identity = 684/738 (92.68%), Postives = 701/738 (94.99%), Query Frame = 1

Query: 1   MLKKLRRNFKKFKTLINKSFNYSSSSSS--KRYKFSS-PSPPLSPPPPPEMSSPMQPFWQ 60
           MLKKL+ NFKK +TLINKSFN SSSSSS  KRYKFSS PSPP   PPP EMSSP QPFWQ
Sbjct: 6   MLKKLKGNFKKIRTLINKSFNNSSSSSSSSKRYKFSSSPSPPPLSPPPLEMSSPSQPFWQ 65

Query: 61  SPPPVAALFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKS 120
           SPPPVAALFPQT+S+VLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKS
Sbjct: 66  SPPPVAALFPQTESSVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKS 125

Query: 121 SLSTVSISYPSMFLNSAFGYQVFNADWTVSENPSSVSQKPHIISSFSDLSLTLDIPSGNL 180
           SLSTVS+SYPSM  NSAFGYQVFNADWTVSENP SVSQKPHIISSFSDLSLTLDIPSGNL
Sbjct: 126 SLSTVSVSYPSMLSNSAFGYQVFNADWTVSENPPSVSQKPHIISSFSDLSLTLDIPSGNL 185

Query: 181 RFFLVRGSPFMTFEVLNNTPISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIYSSSPIN 240
           RFFLVRGSPFMTFEV NNT ISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIY+SSPIN
Sbjct: 186 RFFLVRGSPFMTFEVFNNTQISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIYASSPIN 245

Query: 241 LTHSLSQITSGGFSGIIRIAVLPNPHCETILDRFSSCYPVSGEVNFRNPFCLEYKWEKNG 300
           LTHSLSQITSGGF+GI+RIAVLPNPHCETILDRFSSCYPVSGEVNFRNPF LEYKWEK G
Sbjct: 246 LTHSLSQITSGGFTGIVRIAVLPNPHCETILDRFSSCYPVSGEVNFRNPFSLEYKWEKKG 305

Query: 301 WGNLLMLAHPLHLRLLSGYDSGAVVLDDFKYKSIDGDLVGIVGGSWVLKPDPVSVTWHSI 360
           WGNLLMLAHPLHLRLLSG  S AVVLDDFKYKSIDGDLVG+VGGSW LKPDPV +TWHSI
Sbjct: 306 WGNLLMLAHPLHLRLLSGDGSSAVVLDDFKYKSIDGDLVGVVGGSWALKPDPVYLTWHSI 365

Query: 361 NGVGEEFQSEIISALVKDVEGLKSSPITTTSSYFYGKSIARAARLALIAEEVNFLQVIPE 420
           NGVGEEF SEIISALVKDVEGLKSSPITTTSSYFYGKSIARAARLALIAEEVN+LQVIPE
Sbjct: 366 NGVGEEFHSEIISALVKDVEGLKSSPITTTSSYFYGKSIARAARLALIAEEVNYLQVIPE 425

Query: 421 VRKFLKGAIEPWLHGTFNGNGFLYDGKWGGLVTQQGSTNSGADFGFGVYNDHHYHIGYFL 480
           VRKFLKGAIEPWL GTFNGNGFLYDGKWGGLVTQQGS +SG DFGFGVYNDHHYHIGYFL
Sbjct: 426 VRKFLKGAIEPWLLGTFNGNGFLYDGKWGGLVTQQGSHDSGGDFGFGVYNDHHYHIGYFL 485

Query: 481 YAIAVLVKIDPAWGRKFRPHAYSLMADFMNLSRRSNSTFPRLRCFDLYKLHSWASGLTEF 540
           YAIAVLVKIDPAWGRKF+PHAYSLMADFMNLSRRSNS FPRLRCFDLYKLHSWASGLTEF
Sbjct: 486 YAIAVLVKIDPAWGRKFKPHAYSLMADFMNLSRRSNSMFPRLRCFDLYKLHSWASGLTEF 545

Query: 541 ADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIREGDNLYD 600
           ADGRNQESTSEAVN YYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQI+EGDNLY+
Sbjct: 546 ADGRNQESTSEAVNGYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIKEGDNLYE 605

Query: 601 VDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEILFSDAGFAKELVD 660
            DFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLP+LPITEILFSDA F KELVD
Sbjct: 606 EDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPILPITEILFSDADFVKELVD 665

Query: 661 WTLPSLGREGVGEGWKGFAYALQGVYDKDGVVEKIRNLTGFDDGNSLSNLLWWIHSRRGG 720
           WTLPSLGREGVGEGWKGFAYALQGVYDKDG +EKIRNLTGFDDGNSLSNLLWWIHS RGG
Sbjct: 666 WTLPSLGREGVGEGWKGFAYALQGVYDKDGALEKIRNLTGFDDGNSLSNLLWWIHS-RGG 725

Query: 721 GDEGEGSGGGWKHWWFSH 736
           GDE E  GGGWKHWWFSH
Sbjct: 726 GDEVEACGGGWKHWWFSH 742

BLAST of Lsi03G002200 vs. NCBI nr
Match: gi|700197383|gb|KGN52560.1| (hypothetical protein Csa_5G643880 [Cucumis sativus])

HSP 1 Score: 1381.3 bits (3574), Expect = 0.0e+00
Identity = 684/738 (92.68%), Postives = 701/738 (94.99%), Query Frame = 1

Query: 1   MLKKLRRNFKKFKTLINKSFNYSSSSSS--KRYKFSS-PSPPLSPPPPPEMSSPMQPFWQ 60
           MLKKL+ NFKK +TLINKSFN SSSSSS  KRYKFSS PSPP   PPP EMSSP QPFWQ
Sbjct: 1   MLKKLKGNFKKIRTLINKSFNNSSSSSSSSKRYKFSSSPSPPPLSPPPLEMSSPSQPFWQ 60

Query: 61  SPPPVAALFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKS 120
           SPPPVAALFPQT+S+VLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKS
Sbjct: 61  SPPPVAALFPQTESSVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKS 120

Query: 121 SLSTVSISYPSMFLNSAFGYQVFNADWTVSENPSSVSQKPHIISSFSDLSLTLDIPSGNL 180
           SLSTVS+SYPSM  NSAFGYQVFNADWTVSENP SVSQKPHIISSFSDLSLTLDIPSGNL
Sbjct: 121 SLSTVSVSYPSMLSNSAFGYQVFNADWTVSENPPSVSQKPHIISSFSDLSLTLDIPSGNL 180

Query: 181 RFFLVRGSPFMTFEVLNNTPISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIYSSSPIN 240
           RFFLVRGSPFMTFEV NNT ISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIY+SSPIN
Sbjct: 181 RFFLVRGSPFMTFEVFNNTQISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIYASSPIN 240

Query: 241 LTHSLSQITSGGFSGIIRIAVLPNPHCETILDRFSSCYPVSGEVNFRNPFCLEYKWEKNG 300
           LTHSLSQITSGGF+GI+RIAVLPNPHCETILDRFSSCYPVSGEVNFRNPF LEYKWEK G
Sbjct: 241 LTHSLSQITSGGFTGIVRIAVLPNPHCETILDRFSSCYPVSGEVNFRNPFSLEYKWEKKG 300

Query: 301 WGNLLMLAHPLHLRLLSGYDSGAVVLDDFKYKSIDGDLVGIVGGSWVLKPDPVSVTWHSI 360
           WGNLLMLAHPLHLRLLSG  S AVVLDDFKYKSIDGDLVG+VGGSW LKPDPV +TWHSI
Sbjct: 301 WGNLLMLAHPLHLRLLSGDGSSAVVLDDFKYKSIDGDLVGVVGGSWALKPDPVYLTWHSI 360

Query: 361 NGVGEEFQSEIISALVKDVEGLKSSPITTTSSYFYGKSIARAARLALIAEEVNFLQVIPE 420
           NGVGEEF SEIISALVKDVEGLKSSPITTTSSYFYGKSIARAARLALIAEEVN+LQVIPE
Sbjct: 361 NGVGEEFHSEIISALVKDVEGLKSSPITTTSSYFYGKSIARAARLALIAEEVNYLQVIPE 420

Query: 421 VRKFLKGAIEPWLHGTFNGNGFLYDGKWGGLVTQQGSTNSGADFGFGVYNDHHYHIGYFL 480
           VRKFLKGAIEPWL GTFNGNGFLYDGKWGGLVTQQGS +SG DFGFGVYNDHHYHIGYFL
Sbjct: 421 VRKFLKGAIEPWLLGTFNGNGFLYDGKWGGLVTQQGSHDSGGDFGFGVYNDHHYHIGYFL 480

Query: 481 YAIAVLVKIDPAWGRKFRPHAYSLMADFMNLSRRSNSTFPRLRCFDLYKLHSWASGLTEF 540
           YAIAVLVKIDPAWGRKF+PHAYSLMADFMNLSRRSNS FPRLRCFDLYKLHSWASGLTEF
Sbjct: 481 YAIAVLVKIDPAWGRKFKPHAYSLMADFMNLSRRSNSMFPRLRCFDLYKLHSWASGLTEF 540

Query: 541 ADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIREGDNLYD 600
           ADGRNQESTSEAVN YYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQI+EGDNLY+
Sbjct: 541 ADGRNQESTSEAVNGYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIKEGDNLYE 600

Query: 601 VDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEILFSDAGFAKELVD 660
            DFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLP+LPITEILFSDA F KELVD
Sbjct: 601 EDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPILPITEILFSDADFVKELVD 660

Query: 661 WTLPSLGREGVGEGWKGFAYALQGVYDKDGVVEKIRNLTGFDDGNSLSNLLWWIHSRRGG 720
           WTLPSLGREGVGEGWKGFAYALQGVYDKDG +EKIRNLTGFDDGNSLSNLLWWIHS RGG
Sbjct: 661 WTLPSLGREGVGEGWKGFAYALQGVYDKDGALEKIRNLTGFDDGNSLSNLLWWIHS-RGG 720

Query: 721 GDEGEGSGGGWKHWWFSH 736
           GDE E  GGGWKHWWFSH
Sbjct: 721 GDEVEACGGGWKHWWFSH 737

BLAST of Lsi03G002200 vs. NCBI nr
Match: gi|1009176854|ref|XP_015869652.1| (PREDICTED: probable endo-1,3(4)-beta-glucanase ARB_01444 [Ziziphus jujuba])

HSP 1 Score: 1083.2 bits (2800), Expect = 0.0e+00
Identity = 537/750 (71.60%), Postives = 615/750 (82.00%), Query Frame = 1

Query: 1   MLKKLRRNFKKFKTLINKSF-------NYSSSSSSKRYKFSSPSPP----LSPPPPPEMS 60
           MLKKLRR   + KTLI KSF       +Y +  ++ + +   PSPP    +SPPP P + 
Sbjct: 1   MLKKLRR---RVKTLITKSFKKPHKKKSYKTPETTTQTQSPCPSPPESLTMSPPPEPILK 60

Query: 61  SPMQPFWQSPPPVAALFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEY 120
            P  PF         LFPQTQSTVLPDPS FF+P LL +PLPT+SFFQN+ +KNGDQPEY
Sbjct: 61  LPNSPF---------LFPQTQSTVLPDPSTFFSPDLLQNPLPTSSFFQNYVLKNGDQPEY 120

Query: 121 IHPYLIKSSLSTVSISYPSMFLNSAFGYQVFNADWTVSENPSSV--SQKPHIISSFSDLS 180
           IHPYL+KS  S++++SYPS F NSAF YQ+F AD T+S +  +   SQ+ H++SS+ DLS
Sbjct: 121 IHPYLVKSDSSSLTLSYPSRFFNSAFIYQIFVADLTISASKKTNPDSQRNHVVSSYDDLS 180

Query: 181 LTLDIPSGNLRFFLVRGSPFMTFEVLNNTPISISTIHAILSFSSNSSLTKFTVTLNNNQT 240
           +TLD+P+ NLRFFLVRGSPF+TF V   T ISISTIHAILSFSSN+S+TK+T++LNNNQT
Sbjct: 181 VTLDLPASNLRFFLVRGSPFLTFSVSGGTAISISTIHAILSFSSNNSMTKYTISLNNNQT 240

Query: 241 WLIYSSSPINLTHSLSQITSGGFSGIIRIAVLP--NPHCETILDRFSSCYPVSGEVNFRN 300
           WLIY+SSPINLTHSLS I S GFSGIIRIA+LP  NP  E+ILDRFSSCYPVSG+  F  
Sbjct: 241 WLIYTSSPINLTHSLSSINSDGFSGIIRIAILPDSNPLYESILDRFSSCYPVSGDAAFTK 300

Query: 301 PFCLEYKWEKNGWGNLLMLAHPLHLRLLSGYDSGAVVLDDFKYKSIDGDLVGIVGGSWVL 360
           PF LEY W K GWG+LLMLAHPLHLRLLSG DS   VL++FKYKSIDG+LVG+VG SWVL
Sbjct: 301 PFTLEYTWRKRGWGDLLMLAHPLHLRLLSGDDSDVTVLEEFKYKSIDGELVGVVGDSWVL 360

Query: 361 KPDPVSVTWHSINGVGEEFQSEIISALVKDVEGLKSSPITTTSSYFYGKSIARAARLALI 420
           KP PVSVTWHSINGV  E   EIISAL KDVE L ++PITTTSSYFYGK +ARAAR ALI
Sbjct: 361 KPQPVSVTWHSINGVNGEAHDEIISALRKDVEALNTTPITTTSSYFYGKLVARAARFALI 420

Query: 421 AEEVNFLQVIPEVRKFLKGAIEPWLHGTFNGNGFLYDGKWGGLVTQQGSTNSGADFGFGV 480
           AEEV+FL VIP ++KFLK  IEPWL+GTFNGNGFLYD KWGG+VT+QGS +SGADFGFGV
Sbjct: 421 AEEVSFLDVIPAIKKFLKDTIEPWLNGTFNGNGFLYDRKWGGIVTKQGSVDSGADFGFGV 480

Query: 481 YNDHHYHIGYFLYAIAVLVKIDPAWGRKFRPHAYSLMADFMNLSRRSNSTFPRLRCFDLY 540
           YNDHHYH+GYFLY IAVL KIDPAWGRK+RP AY+LMADFMNL RRSNS +PRLRCFDLY
Sbjct: 481 YNDHHYHLGYFLYGIAVLAKIDPAWGRKYRPRAYALMADFMNLGRRSNSNYPRLRCFDLY 540

Query: 541 KLHSWASGLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTALEIKAAQT 600
           KLHSWA GLTEFADGRNQESTSEAVNAYYSAAL+GLAYGDTHL + GS L A EI+AAQ 
Sbjct: 541 KLHSWAGGLTEFADGRNQESTSEAVNAYYSAALMGLAYGDTHLVATGSILAAFEIQAAQM 600

Query: 601 WWQIREGDNLYDVDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEIL 660
           WW +REGDNLY+ DF REN++VGVLW+NKRDSGLWFAPPDW+ECRLGIQVLPLLPITE L
Sbjct: 601 WWHVREGDNLYEEDFTRENRMVGVLWANKRDSGLWFAPPDWKECRLGIQVLPLLPITEAL 660

Query: 661 FSDAGFAKELVDWTLPSLGREGVGEGWKGFAYALQGVYDKDGVVEKIRNLTGFDDGNSLS 720
           FSD GF K+LV WTLP+L REGVGEGWKGF YA++G+YDK+G + KIR+L GFDDGNSLS
Sbjct: 661 FSDVGFVKDLVRWTLPALQREGVGEGWKGFVYAMEGIYDKEGALGKIRSLNGFDDGNSLS 720

Query: 721 NLLWWIHSRRGGGDEGEGSGGGWKHWWFSH 736
           NLLWWIHSR    +E EG  GG K+ WF H
Sbjct: 721 NLLWWIHSR--DEEEEEGCEGG-KYCWFGH 735

BLAST of Lsi03G002200 vs. NCBI nr
Match: gi|703103793|ref|XP_010097822.1| (Endo-1,3(4)-beta-glucanase 1 [Morus notabilis])

HSP 1 Score: 1069.3 bits (2764), Expect = 3.0e-309
Identity = 533/748 (71.26%), Postives = 612/748 (81.82%), Query Frame = 1

Query: 1   MLKKLRRNFKKFKTLINKSFN-------YSSSSSSKRYKFSSPSPP--LSPPPPPEMSSP 60
           MLKKLRR   + KTL+ K+F        +S+ S S   +  SPS P  +  P PP+MS  
Sbjct: 1   MLKKLRR---RVKTLVTKTFKKPRRRPEFSTPSPSTPVEVPSPSTPEEVVEPEPPKMS-- 60

Query: 61  MQPFWQSPPPVAALFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIH 120
           +   +Q       LFP+ QSTV+ DPS FF+P LLS+PLPTNSFFQNF +KNGDQPEYIH
Sbjct: 61  LSSSFQKRSNAPFLFPKAQSTVVSDPSLFFSPDLLSTPLPTNSFFQNFVLKNGDQPEYIH 120

Query: 121 PYLIKSSLSTVSISYPSMFLNSAFGYQVFNADWTVS--ENPSSVSQKPHIISSFSDLSLT 180
           PYL+KSS S++S+ YPS   NSAF YQ+FNAD TVS  E  +  SQKPH+ISSF+DLSLT
Sbjct: 121 PYLVKSSNSSLSVCYPSRLSNSAFLYQIFNADLTVSAAEKTAPDSQKPHLISSFNDLSLT 180

Query: 181 LDIPSGNLRFFLVRGSPFMTFEVLNNTPISISTIHAILSFSSNSSLTKFTVTLNNNQTWL 240
           LDIPS NLRFFLVRGSPF+T      T ISISTIHAILSFSSN+S TK+TV LNNNQTWL
Sbjct: 181 LDIPSSNLRFFLVRGSPFLTCTASAKTEISISTIHAILSFSSNNSQTKYTVKLNNNQTWL 240

Query: 241 IYSSSPINLTHSLSQITSGGFSGIIRIAVLP--NPHCETILDRFSSCYPVSGEVNFRNPF 300
           IY+SSPI+ +HSLS +TS GFSGI+RIA+LP  NP  E ILDRFSSCYPVSGEV+F  PF
Sbjct: 241 IYTSSPISFSHSLSLLTSNGFSGILRIAILPDSNPEFEAILDRFSSCYPVSGEVSFTKPF 300

Query: 301 CLEYKWEKNGWGNLLMLAHPLHLRLLSGYDSGAVVLDDFKYKSIDGDLVGIVGGSWVLKP 360
           CLEYKWEK GWG+LLMLAHPLHL+LL G D G  VL++FKYKSIDGDLVG+VG SW LKP
Sbjct: 301 CLEYKWEKKGWGDLLMLAHPLHLKLLKGDDRGVTVLEEFKYKSIDGDLVGVVGDSWTLKP 360

Query: 361 DPVSVTWHSINGVGEEFQSEIISALVKDVEGLKSSPITTTSSYFYGKSIARAARLALIAE 420
           DPVSVTWHSI GV EE   EI++AL KDVE L ++ ITT +SYFYGK IARAAR ALIAE
Sbjct: 361 DPVSVTWHSIEGVKEEAYEEIVAALQKDVEALDAAAITTNASYFYGKLIARAARFALIAE 420

Query: 421 EVNFLQVIPEVRKFLKGAIEPWLHGTFNGNGFLYDGKWGGLVTQQGSTNSGADFGFGVYN 480
           EV F  VIP++RKFLK AIEPWL GTFNGNGFLY+ KWGG++T+QG  + GADFGFG+YN
Sbjct: 421 EVRFFDVIPKIRKFLKEAIEPWLDGTFNGNGFLYEEKWGGIITKQGQGDGGADFGFGIYN 480

Query: 481 DHHYHIGYFLYAIAVLVKIDPAWGRKFRPHAYSLMADFMNLSRRSNSTFPRLRCFDLYKL 540
           DHH+H+G+FLYAIAVL KIDPAWGRK++P AYS+MADFMNLSRR+ S +PRLRCFDLYKL
Sbjct: 481 DHHFHLGHFLYAIAVLAKIDPAWGRKYKPQAYSMMADFMNLSRRAKSNYPRLRCFDLYKL 540

Query: 541 HSWASGLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWW 600
           HSWA GLTEFADGRNQES+SEAVNAYYSAAL+GLAYGDTHL +IGS LTALEI+AAQ WW
Sbjct: 541 HSWAGGLTEFADGRNQESSSEAVNAYYSAALMGLAYGDTHLVAIGSLLTALEIQAAQMWW 600

Query: 601 QIREGDNLYDVDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEILFS 660
            ++EGD LYD DF REN+VVGVLWSNKRDSGLWFAP  WRECRLGIQVLPLLPITE+LFS
Sbjct: 601 HVKEGDKLYDEDFTRENRVVGVLWSNKRDSGLWFAPAAWRECRLGIQVLPLLPITEVLFS 660

Query: 661 DAGFAKELVDWTLPSLGREGVGEGWKGFAYALQGVYDKDGVVEKIRNLTGFDDGNSLSNL 720
           D GF +ELV+WTLP+L REGVGEGWKGF YAL+G+YDK+  ++KI++L GFDDGNSL+NL
Sbjct: 661 DVGFTRELVNWTLPALQREGVGEGWKGFVYALEGIYDKETALQKIKSLNGFDDGNSLTNL 720

Query: 721 LWWIHSRRGGGDEGEGSGGGWKHWWFSH 736
           LWWIHSR     E E  GGG KH WF+H
Sbjct: 721 LWWIHSR---DQEKEEFGGGGKHCWFAH 740