Lsi03G002120 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi03G002120
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Proline dehydrogenase
Location	chr03 : 2724191 .. 2728268 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTCAACGTTTTGGCACAATATTCGATAAGATTTTGCTGCCACGTCCGTTTTCTTATGAATAACACATTGCCACGTGGTTATTTGATGGCTTTATTTCTTCAATAGTTTTTCCATCTAAATCTTTTTCTTGTGGAAAAGAAAAGTAGACCAAGCAACTAAAATAAATGAAAGGAAGGGAAATGGTAATAATCTTGAAAAATAGTAATAAAATCCAATCTAATTTTTTAAAATAAGGTTTATAAACACTATTTCACCCATAATTCTTCGTATGTTTTGATTGATTGTGACATATTTTGAACACTTTTTTAATTTGACAACTGATAAAAGTAATGTTGTATTTGATTGATAGGCAAGTTTCGTGGTGACTAAAGTGAGTGCAATTTGTCCATTGAGATTACTGGAGAGAGTTAGCGACTTATTAAGATGGCAACAAAAGAACCCTAGTTTCAATCTTCCATGGAAGCTCGAAACTTTGCCAATTTTCTCAGAATCAAGCCCTCTTTACCACACTCTTGAACAACCAAAACCCTTAACAAATGAAGAAGAAAAAAGCCTCCAAATGAGCCATGAAAGGCTAATGAAAATTTGTGAAAGTTGTGTTGATGCCAATATTCCTTTAGCCATTGATGCTGAACATACCAAAGTTCAACCAGCCATTGATTACTTCACTTATTCAGCTGCCATTATTCACAACAAAGACCACAATCCAATAGTTTATGGAACAATTCAAGCCTATCTAAAAGATGCAAAAGATAGACTTTTGTTAGTTACCAAAGAAGCTACCAAAATGAAAGTTCCACTTGGTATTAAGCTTGTGAGAGGTGCTTATATGTCAAGTGAATCCAAACTTGCTTCTTCTCTTGGCTTTGAATCTCCAATTCACAATACAATTCAAGATACACATTTTTGCTACAATACTTGTGCATCTTTCTTGCTTGACAACATCGCCAAAGGCTCGAGTGGAGCTATTCTTGCAACCCATAACATCGAATCAGGTAATGAATTATGCTCTATGCTCTATGCTCTTCTTATTTCCAAACCGACCCAGTTGTTTGATAACCATTTCGGTTTCTTTTGAATAGGGAAATTGGCAGCTAGTAAAGCATATGAGATAGGGATAGGAAAGTTGAAGCAAAAGCTTGAGTTTGCTCAATTGTATGGAATGTCAGAAGCACTTTCTTTTGGGTTGAGAAATGCAGGGTTTCAAGTGAGTAAATACATGCCATTTGGACCTGTGGATATGGTAATGCCATACCTTTTAAGAAGAGCTGAAGAGAATAGAGGTCTCTTATCAACTTCAAATTTGGATAGACAACTTATGAGGTAATTCAAAACATATTTAATTAATTTTCATATAGTATGTTGAAAATTTTGTTAAACTATTTACAGAAAAATAACAAAAAAAAAAAAAAAAAACACGGATAGACATTATCAACTTCTATCAGCATCTATCAGTGATAAAACTTCTATCATTTCTATCCGCGTCTATCACAACTATCTAAACATTTTACTATTTTGTGTAAATAGTTTTTTTTATTTTTTTATTTTTAAAAATTCTCTTAGTATATATATGGATTTGACACATTTGAAATTTCTTGATTCTTTGCAGAAGGGAGATGGGAAGGAGGATGAAAGAGTACTTTAGGTGAAAAGAAAGGGCCTTTTAGCAATTTCAACAACCTACAACACCAAGATTTGCAATTTTTGTTTATAGGATCACACATGGTTTTAAAAACCTACTTTGTTTTGTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTAAAATCAAACAAAAGTTAAATTGCAAAACCACTTTGGTGAACAAAAAATATAATGTTTACTTAAAATATATATGTGGTTAAGTTACAAATTTATAATTCTTTTTTAGGATAAAAATTATGATGTTGGAAATCAAATTTAGAACTGTTTTCAAATATAAAAAAATGAGTTAAATTATTTACGAATATAGAAATTTTTTACTACATATTAAGGATAAACCGATTATAAAACTTGAACAATAAATAAGAATAGTCAAGTCAGTGAAATTTTTTAATATTTATAAATAATCTGATTTTTTTTGTTTAACAGTAATTTCTCAAAAAAAGTAAAAAAAGCGGTTGTGAGTTCAATCTATAGTGGCCATATTTAATATCCTACGATAAGTTGGCAGGCTGCCTGTTATAGGGTCGGGTGGATTGTCTCGTGAGATTAATAGAGATGGGCGTAAGTTGTCCAGACACTCACGGATATAAAAAAAGAAAAAACACATTAATTTCCCACAAAAACACAGTAATTTCCCACAAAATTAAGTATATTTATCATAAATAATTTAATTCTTAATTTAACTGAAAAAATATTCTCACCCACTCTTCTTCCCAACCCGTCTGAAATCGGACGCTCTCTCTGCTCTCTCTCCCTCCTTAACTTACCGCCGGCCGCCTTCTCTCTCCGTCTCCGGCATTCAGGGATCAGTTTCTCCTGTCTCTCAAGAAACCGATCGCTTCACAGAATACAGCAACCAACAGTAAGTCCTACAAATAGAATAATCGGAAGCATTTTTTAATAATTTTACTTCAATTGAGAGTATGATTGGAGGCTGTTTTTGGATCCTTTAAGTGTCATCTTACTTGTTCAATTGATCAATTCAACCCTATCTTGTGTATCGTATTCGTATTCTACGCCTCAATTTGGAGATTATATATATAATTTTCTTTTATTTATTCAACACTATCAGTGAATTAGATTCTCGAGAATCTCCTCTTTTGGCTTTGGAGTTTTTAACATTTCCTCTGTCGACACTGAGGCAGAAAGCAGAATAAAGTGGCAATGCGGAAAGTGCTCGTAACTTGTTATAGATCATCCATATCAGAATTTTTCAATTCCGTTTTCTTCTGGGTTCAAATTCAAACCTACCTGCGAATTTAATTTATGATGGTGGTGCAGAAGCCCTAGAGGTACGAAATTCCTTTGCTATCTTCAACGATCTCAATCAAATATACAAAGATATAGGGTTTGCGAAATTGTTATTCTTTACCTTTCTGTTATGGGATGTCGAGGTTGAATTTGCTTAGAAGCGATGATATGTGCTTCATTCGATGAAATTCGTTTCCCAAAACATGGATGTTTCGAAGAAAATCCATTAATTTTTCCTTAAATAAGTATTATTGAGCTTCTGCATCATTGACAGGCTGCGTCTTTCTTTTTAGGGATTTCTGGGTAG

mRNA sequence

ATGGTCAACGTTTTGGCACAATATTCGATAAGATTTTGCTGCCACGCAAGTTTCGTGGTGACTAAAGTGAGTGCAATTTGTCCATTGAGATTACTGGAGAGAGTTAGCGACTTATTAAGATGGCAACAAAAGAACCCTAGTTTCAATCTTCCATGGAAGCTCGAAACTTTGCCAATTTTCTCAGAATCAAGCCCTCTTTACCACACTCTTGAACAACCAAAACCCTTAACAAATGAAGAAGAAAAAAGCCTCCAAATGAGCCATGAAAGGCTAATGAAAATTTGTGAAAGTTGTGTTGATGCCAATATTCCTTTAGCCATTGATGCTGAACATACCAAAGTTCAACCAGCCATTGATTACTTCACTTATTCAGCTGCCATTATTCACAACAAAGACCACAATCCAATAGTTTATGGAACAATTCAAGCCTATCTAAAAGATGCAAAAGATAGACTTTTGTTAGTTACCAAAGAAGCTACCAAAATGAAAGTTCCACTTGGTATTAAGCTTGTGAGAGGTGCTTATATGTCAAGTGAATCCAAACTTGCTTCTTCTCTTGGCTTTGAATCTCCAATTCACAATACAATTCAAGATACACATTTTTGCTACAATACTTGTGCATCTTTCTTGCTTGACAACATCGCCAAAGGCTCGAGTGGAGCTATTCTTGCAACCCATAACATCGAATCAGGTAATGAATTATGCTCTATGCTCTATGCTCTTCTTATTTCCAAACCGACCCAGTTGTTTGATAACCATTTCGGGTTTCAAGTGAGTAAATACATGCCATTTGGACCTGTGGATATGGTAATGCCATACCTTTTAAGAAGAGCTGAAGAGAATAGAGGGTCGGGTGGATTGTCTCGTGAGATTAATAGAGATGGGCGGATCAGTTTCTCCTGTCTCTCAAGAAACCGATCGCTTCACAGAATACAGCAACCAACAGCTGCGTCTTTCTTTTTAGGGATTTCTGGGTAG

Coding sequence (CDS)

ATGGTCAACGTTTTGGCACAATATTCGATAAGATTTTGCTGCCACGCAAGTTTCGTGGTGACTAAAGTGAGTGCAATTTGTCCATTGAGATTACTGGAGAGAGTTAGCGACTTATTAAGATGGCAACAAAAGAACCCTAGTTTCAATCTTCCATGGAAGCTCGAAACTTTGCCAATTTTCTCAGAATCAAGCCCTCTTTACCACACTCTTGAACAACCAAAACCCTTAACAAATGAAGAAGAAAAAAGCCTCCAAATGAGCCATGAAAGGCTAATGAAAATTTGTGAAAGTTGTGTTGATGCCAATATTCCTTTAGCCATTGATGCTGAACATACCAAAGTTCAACCAGCCATTGATTACTTCACTTATTCAGCTGCCATTATTCACAACAAAGACCACAATCCAATAGTTTATGGAACAATTCAAGCCTATCTAAAAGATGCAAAAGATAGACTTTTGTTAGTTACCAAAGAAGCTACCAAAATGAAAGTTCCACTTGGTATTAAGCTTGTGAGAGGTGCTTATATGTCAAGTGAATCCAAACTTGCTTCTTCTCTTGGCTTTGAATCTCCAATTCACAATACAATTCAAGATACACATTTTTGCTACAATACTTGTGCATCTTTCTTGCTTGACAACATCGCCAAAGGCTCGAGTGGAGCTATTCTTGCAACCCATAACATCGAATCAGGTAATGAATTATGCTCTATGCTCTATGCTCTTCTTATTTCCAAACCGACCCAGTTGTTTGATAACCATTTCGGGTTTCAAGTGAGTAAATACATGCCATTTGGACCTGTGGATATGGTAATGCCATACCTTTTAAGAAGAGCTGAAGAGAATAGAGGGTCGGGTGGATTGTCTCGTGAGATTAATAGAGATGGGCGGATCAGTTTCTCCTGTCTCTCAAGAAACCGATCGCTTCACAGAATACAGCAACCAACAGCTGCGTCTTTCTTTTTAGGGATTTCTGGGTAG

Protein sequence

MVNVLAQYSIRFCCHASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKPLTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNPIVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNTIQDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFDNHFGFQVSKYMPFGPVDMVMPYLLRRAEENRGSGGLSREINRDGRISFSCLSRNRSLHRIQQPTAASFFLGISG

Homology

[Close]

BLAST of Lsi03G002120 vs. Swiss-Prot
Match: PROD2_ARATH (Proline dehydrogenase 2, mitochondrial OS=Arabidopsis thaliana GN=POX2 PE=2 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 5.3e-85
Identity = 165/299 (55.18%), Postives = 208/299 (69.57%), Query Frame = 1


Query: 15  HASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPK 74
           H S VV K++AICP+ +L+RVSDLLRWQ KNP+F LPWKL + P+FS  SPLYHT  +P+
Sbjct: 168 HLSSVVVKITAICPMNVLKRVSDLLRWQYKNPNFKLPWKLNSFPVFSGLSPLYHTTSEPE 227

Query: 75  PLTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHN 134
           PLT EEE+ L+ +HERL  +C  C ++N+PL IDAE T +QPAIDY  Y +AI+ N D +
Sbjct: 228 PLTVEEERELEKAHERLKSVCLRCQESNVPLLIDAEDTILQPAIDYMAYWSAIMFNSDKD 287

Query: 135 -PIVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIH 194
            PIVY TIQAYLKDA +RL L  +E+ KM VP+G KLVRGAYMSSE+KLA SLG++SP+H
Sbjct: 288 RPIVYNTIQAYLKDAGERLHLALRESEKMNVPIGFKLVRGAYMSSEAKLADSLGYKSPVH 347

Query: 195 NTIQDTHFCYNTCASFLLDNIAKGSS-GAILATHNIESGNELCSMLYALLISKP------ 254
           +TIQ+TH CYN C SFL++  + GS    ILATHN +SG         L I+K       
Sbjct: 348 DTIQNTHDCYNDCMSFLMEKASNGSGIAVILATHNTDSGKLGARKASELGINKENGKIEF 407

Query: 255 TQLFD---------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
            QL+             GF VSKYMP+GPVD  +PYL+RRA ENRG   +G L R++ R
Sbjct: 408 AQLYGMSDALSFGLKRAGFNVSKYMPYGPVDTAIPYLIRRAYENRGMMSTGALDRQLMR 466

[Close]

BLAST of Lsi03G002120 vs. Swiss-Prot
Match: PROD1_ARATH (Proline dehydrogenase 1, mitochondrial OS=Arabidopsis thaliana GN=POX1 PE=2 SV=2)

HSP 1 Score: 307.4 bits (786), Expect = 1.9e-82
Identity = 162/299 (54.18%), Postives = 205/299 (68.56%), Query Frame = 1


Query: 15  HASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPK 74
           H S VV K++AICP+ LL+RVSDLLRW+ K+P+F L WKL++ P+FSESSPLYHT  +P+
Sbjct: 189 HFSSVVVKITAICPISLLKRVSDLLRWEYKSPNFKLSWKLKSFPVFSESSPLYHTNSEPE 248

Query: 75  PLTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHN 134
           PLT EEE+ L+ +H R+ +IC  C ++N+PL IDAE T +QPAIDY  YS+AI+ N D +
Sbjct: 249 PLTAEEERELEAAHGRIQEICRKCQESNVPLLIDAEDTILQPAIDYMAYSSAIMFNADKD 308

Query: 135 -PIVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIH 194
            PIVY TIQAYL+DA +RL L  + A K  VP+G KLVRGAYMSSE+ LA SLG +SP+H
Sbjct: 309 RPIVYNTIQAYLRDAGERLHLAVQNAEKENVPMGFKLVRGAYMSSEASLADSLGCKSPVH 368

Query: 195 NTIQDTHFCYNTCASFLLDNIAKGSS-GAILATHNIESGNELCSMLYALLISKP------ 254
           +TIQDTH CYN C +FL++  + GS  G +LATHN +SG         L I K       
Sbjct: 369 DTIQDTHSCYNDCMTFLMEKASNGSGFGVVLATHNADSGRLASRKASDLGIDKQNGKIEF 428

Query: 255 TQLFD---------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
            QL+             GF VSKYMPFGPV   +PYLLRRA ENRG   +G   R++ R
Sbjct: 429 AQLYGMSDALSFGLKRAGFNVSKYMPFGPVATAIPYLLRRAYENRGMMATGAHDRQLMR 487

[Close]

BLAST of Lsi03G002120 vs. Swiss-Prot
Match: PROD_DICDI (Proline dehydrogenase 1, mitochondrial OS=Dictyostelium discoideum GN=prodh PE=3 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 2.6e-31
Identity = 99/295 (33.56%), Postives = 150/295 (50.85%), Query Frame = 1


Query: 17  SFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKPL 76
           SF   K + +    +LE+++ L+         N+   +  LPI S +SPL   L Q   L
Sbjct: 265 SFSCVKFTGLVTPSVLEKMNTLVS--------NVTTNVSELPIESFNSPLDFYLNQSSSL 324

Query: 77  ---------TNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAI 136
                    T+++ K ++    R+ KI + C    +P+ +DAE +  Q AI + T S +I
Sbjct: 325 MKQGSEPLLTSKDIKEIKEFFNRMDKIFQLCHQRGVPILVDAEQSYYQVAIHHLTMSYSI 384

Query: 137 IHNKDHNPIVYGTIQAYLKDAKDRLL--LVTKEATKMKVPLGIKLVRGAYMSSESKLASS 196
            +NK+  PI+Y T Q YL +  + L        + K    LG K+VRGAYM +ES+ +  
Sbjct: 385 KYNKE-KPIIYNTYQMYLVNGMNVLKQHFELSSSQKFNFKLGAKIVRGAYMVTESERSQR 444

Query: 197 LGFESPIHNTIQDTHFCYNTCASFLLDNIAK--GSSGAILATHNIESGNELCSML--YAL 256
           L  E+P+  TIQDTH  YNT   FLL+ I     S G ++A+HN +S N    ++  Y +
Sbjct: 445 LSTENPVLPTIQDTHKSYNTALDFLLNQIKSDPNSIGLMIASHNEDSINLGTKLIKQYKI 504

Query: 257 LISKPTQLFDNHFGF-------------QVSKYMPFGPVDMVMPYLLRRAEENRG 284
             + P   F   FG              ++ KY+PFGPV+ V+PYL+RR  EN+G
Sbjct: 505 DPTNPNIQFGQLFGMADFLSFNLVDQHQRIFKYVPFGPVEEVLPYLIRRMHENKG 550

[Close]

BLAST of Lsi03G002120 vs. Swiss-Prot
Match: PROD_CAEEL (Proline dehydrogenase 1, mitochondrial OS=Caenorhabditis elegans GN=B0513.5 PE=3 SV=2)

HSP 1 Score: 112.1 bits (279), Expect = 1.2e-23
Identity = 77/261 (29.50%), Postives = 128/261 (49.04%), Query Frame = 1


Query: 76  LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNP 135
           L+NEEE+  +    R + + E  ++  + + +DAE T +QPAI   T      +NK    
Sbjct: 359 LSNEEEQEFRNMVRRTLDVAEYAIEKGVRIMVDAEQTYLQPAISKITIEMMKKYNKGRGN 418

Query: 136 IVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNT 195
           I + T QAYLK     +    + A +     G KLVRGAYM  E   A ++G+E PI++ 
Sbjct: 419 I-FNTYQAYLKGTLQNMEADMQVARREGWHFGAKLVRGAYMEQERARAKAIGYEDPINDN 478

Query: 196 IQDTHFCYNTCASFLLDNI---AKGSSGAILATHNIESGNELCSMLYALLISKP------ 255
            + T   Y +C + + D +    K +   ++A+HN ++     +++    IS        
Sbjct: 479 FEATSKMYESCLTRIADEVHRRGKTNVSVMVASHNEDTVRFALNLMKEKCISPSERVMCM 538

Query: 256 TQLFD---------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRGSGGLSREINRDGRI 315
            QL+             GF V KY+P+GPV+ V+PYL RRA EN   G + ++ N++  +
Sbjct: 539 AQLYGMCDQVSFSLGQAGFSVYKYLPYGPVEEVLPYLSRRALEN---GSVLKKANKERDL 598

Query: 316 SFSCLSRNRSLHRIQQPTAAS 319
            +  L R  S    +  +++S
Sbjct: 599 LWKELKRRISSGEFKARSSSS 615

[Close]

BLAST of Lsi03G002120 vs. Swiss-Prot
Match: PROD_DROME (Proline dehydrogenase 1, mitochondrial OS=Drosophila melanogaster GN=slgA PE=2 SV=2)

HSP 1 Score: 104.8 bits (260), Expect = 1.9e-21
Identity = 83/288 (28.82%), Postives = 128/288 (44.44%), Query Frame = 1


Query: 51  PWKLETLPIFSESSPLYHTLEQPKPLTNE-----------EEKSLQMSHERLMKICESCV 110
           PW      I  E S L  T   P P T +           EE+  +    RL  I ++  
Sbjct: 384 PWS----GIVDEDSQLSDTFRVPDPQTGQMRRLISQIPPKEEEMFRNMIRRLNTIVKAAA 443

Query: 111 DANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNPIVYGTIQAYLKDAKDRLLLVTKEA 170
           D ++ + +DAE T  QPAI   T      +NKD   IV+ T Q YL++    +    ++A
Sbjct: 444 DLDVRIMVDAEQTYFQPAISRITLEMMRKYNKD-KAIVFNTYQCYLRETFREVNTDLEQA 503

Query: 171 TKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNTIQDTHFCYNTCASFLLDNIAKGSS 230
            +     G KLVRGAYM  E   A SLG+  P++ T + T   Y+   S  L  I     
Sbjct: 504 KRQNFYFGAKLVRGAYMDQERDRAKSLGYPDPVNPTFEATTDMYHRTLSECLRRIKLMKD 563

Query: 231 --------GAILATHNIESGNELCSMLYALLISKPTQLFD---------------NHFGF 290
                   G ++A+HN ++       +  + IS   ++                    G+
Sbjct: 564 CDDDARKIGIMVASHNEDTVRFAIQQMKEIGISPEDKVICFGQLLGMCDYITFPLGQAGY 623

Query: 291 QVSKYMPFGPVDMVMPYLLRRAEENRGSGGLSREINRDGRISFSCLSR 305
              KY+P+GPV+ V+PYL RRA+EN+   G+ ++I ++ R+  S + R
Sbjct: 624 SAYKYIPYGPVEEVLPYLSRRAQENK---GVLKKIKKEKRLLLSEIRR 663

[Close]

BLAST of Lsi03G002120 vs. TrEMBL
Match: A0A0A0KXN4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G643300 PE=4 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 1.0e-127
Identity = 239/296 (80.74%), Postives = 254/296 (85.81%), Query Frame = 1


Query: 16  ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKP 75
           ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKL+TLPIFSESSPLYHTLEQP+P
Sbjct: 185 ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLEQPEP 244

Query: 76  LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNP 135
           LT EEEKSLQMSHERLMKIC+SCVDAN+PLAIDAEHTKVQPAIDYFTYSAAIIHNKD NP
Sbjct: 245 LTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKDRNP 304

Query: 136 IVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNT 195
           IVYGTIQAYLKDAKDRLLL  KEA+K+KVPLGIKLVRGAYMSSESKLASSLGFESPIH+T
Sbjct: 305 IVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGFESPIHDT 364

Query: 196 IQDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFD---- 255
           IQDTH CYNTCASFLLD+IAKGS+GAILATHN+ESG    S  Y + I K  Q  +    
Sbjct: 365 IQDTHSCYNTCASFLLDDIAKGSTGAILATHNVESGKLAASRAYEIGIGKLKQKLEFAQL 424

Query: 256 -----------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
                       + GFQVSKYMPFGPVDMVMPYLLRRAEENRG   +  L RE+ R
Sbjct: 425 YGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRELMR 480

[Close]

BLAST of Lsi03G002120 vs. TrEMBL
Match: V9M5A2_MANES (Proline oxidase OS=Manihot esculenta GN=ProDH PE=2 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 9.7e-102
Identity = 191/295 (64.75%), Postives = 227/295 (76.95%), Query Frame = 1


Query: 17  SFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKPL 76
           S VV K++AICPL LLERV+DLLRWQ+K+PSFNLPWK  T PIFSES+PLYHTL++P+PL
Sbjct: 190 SAVVVKITAICPLNLLERVADLLRWQEKDPSFNLPWKQNTFPIFSESTPLYHTLKKPEPL 249

Query: 77  TNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNPI 136
           T EEE+ L +  ++L+K+C++C +AN+PL IDAEHTKVQPAIDYFTYS+AI +NKD NPI
Sbjct: 250 TPEEEQHLLLGQQKLLKLCQACAEANVPLVIDAEHTKVQPAIDYFTYSSAIKYNKDDNPI 309

Query: 137 VYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNTI 196
           VYGTIQAYLKDAK+RLLL TK A KM +P+G KLVRGAYMSSE KLASSLG++SPIHN+I
Sbjct: 310 VYGTIQAYLKDAKERLLLATKAADKMGLPMGFKLVRGAYMSSERKLASSLGYQSPIHNSI 369

Query: 197 QDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFD----- 256
           Q TH CYN CASFLL+ IA  S G ILATHN+ESG         L I    Q  +     
Sbjct: 370 QQTHDCYNDCASFLLEAIADRSHGLILATHNVESGTLAAKKAEELGIGNGNQRLEFAQLY 429

Query: 257 ----------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
                      + GFQVSKY+PFGPV+MV+PYLLRRAEENRG   + GL RE+ R
Sbjct: 430 GMAEALSFGLRNAGFQVSKYLPFGPVEMVIPYLLRRAEENRGLLSASGLDRELTR 484

[Close]

BLAST of Lsi03G002120 vs. TrEMBL
Match: A0A151RH61_CAJCA (Uncharacterized protein OS=Cajanus cajan GN=KK1_036702 PE=4 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 2.8e-101
Identity = 187/295 (63.39%), Postives = 230/295 (77.97%), Query Frame = 1


Query: 17  SFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKPL 76
           SF V K++AICP+ LLERVSDLLRWQQK+PSF LPWK ++LPIF+ESSPLYHT ++P+PL
Sbjct: 184 SFAVVKITAICPMALLERVSDLLRWQQKDPSFILPWKQDSLPIFAESSPLYHTQKRPEPL 243

Query: 77  TNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNPI 136
           T EEE  L+++++RL+++C+ CVDANIPL +DAEHT VQPAIDYFTYS+AI+HNKD  PI
Sbjct: 244 TPEEESDLELANQRLLELCQKCVDANIPLLVDAEHTTVQPAIDYFTYSSAIMHNKDDKPI 303

Query: 137 VYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNTI 196
           V+GTIQ YLKDAK+RLLL TK A KM +P+G KLVRGAYMS+ESKLA SLG+ SPIHNTI
Sbjct: 304 VFGTIQTYLKDAKERLLLTTKAAEKMGIPMGFKLVRGAYMSTESKLAESLGYASPIHNTI 363

Query: 197 QDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKP------TQLF 256
           +DTH C+N C+SFLL+ +A G    +LATHNIESG    +  + L I K        QL+
Sbjct: 364 EDTHNCFNRCSSFLLEKVANGPGSVVLATHNIESGKLAVAKAHELGIEKVKHKLEFAQLY 423

Query: 257 D---------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
                     ++ GFQVSKYMPFGPV+MVMPYLLRRAEENRG   + G  R++ R
Sbjct: 424 GMSEALSFGLSNAGFQVSKYMPFGPVEMVMPYLLRRAEENRGLLAASGFDRQLMR 478

[Close]

BLAST of Lsi03G002120 vs. TrEMBL
Match: A0A0B2Q656_GLYSO (Proline dehydrogenase 2, mitochondrial OS=Glycine soja GN=glysoja_046995 PE=4 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 3.7e-101
Identity = 187/295 (63.39%), Postives = 229/295 (77.63%), Query Frame = 1


Query: 17  SFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKPL 76
           SFV+ K++AICP+ LLER+SDLLRWQQK+PSF LPWK ++LPIF+ESSPLYHT ++P+PL
Sbjct: 96  SFVIVKITAICPMALLERMSDLLRWQQKDPSFVLPWKQDSLPIFAESSPLYHTQKRPEPL 155

Query: 77  TNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNPI 136
           T EEE  LQ++++RL+++C+ C +AN+PL +DAEHT VQPAIDYFTYS++I HNKD NPI
Sbjct: 156 TPEEESDLQLANQRLLELCQKCEEANMPLLVDAEHTTVQPAIDYFTYSSSIRHNKDDNPI 215

Query: 137 VYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNTI 196
           V+GTIQ YLKDAK+RLLL TK A KM VP+G KLVRGAYMS+ESKLA S G+ SPIHNTI
Sbjct: 216 VFGTIQTYLKDAKERLLLTTKAAEKMGVPMGFKLVRGAYMSTESKLAESFGYASPIHNTI 275

Query: 197 QDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKP------TQLF 256
           QDTH C+N C+SFLL+ IA G    +LATHNIESG    +  Y L + K        QL+
Sbjct: 276 QDTHNCFNDCSSFLLEKIANGPGSVVLATHNIESGKLAATKAYELGVGKVNHKLEFAQLY 335

Query: 257 D---------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
                     ++ GFQVSKYMPFGPVDMVMPYLLRRAEENRG   + G  R++ R
Sbjct: 336 GMSEALSFGLSNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLAASGFDRQLMR 390

[Close]

BLAST of Lsi03G002120 vs. TrEMBL
Match: M5X4N6_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004722mg PE=4 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 1.1e-100
Identity = 176/282 (62.41%), Postives = 228/282 (80.85%), Query Frame = 1


Query: 17  SFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKPL 76
           SF++ K++AICP+R+LERVSDLLRWQ K+PSF LPWKL+TLPIFS++SPLYHTLE+P+PL
Sbjct: 188 SFIIMKITAICPMRVLERVSDLLRWQHKDPSFKLPWKLDTLPIFSDNSPLYHTLEKPEPL 247

Query: 77  TNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNPI 136
           T +EE+ L++ H+RL+K+ + C +AN+PL++DAE++ VQPAIDY TYS+AII+NKD NPI
Sbjct: 248 TPDEERDLELVHQRLLKLSQKCSEANVPLSVDAEYSSVQPAIDYLTYSSAIIYNKDDNPI 307

Query: 137 VYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNTI 196
           VYGTIQAYLKDAK+RLLL TK A  M +P+G K+VRGAYMSSE+K+ASSLG++SPIH++I
Sbjct: 308 VYGTIQAYLKDAKERLLLATKAADDMGIPMGFKVVRGAYMSSEAKVASSLGYKSPIHDSI 367

Query: 197 QDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFD----- 256
           +DTH CYN CASF+L+ IA GS G +LATHN+ESG    +  + + I K  Q  +     
Sbjct: 368 EDTHACYNDCASFMLEKIANGSGGVVLATHNVESGKLAVAKAHEIGIGKVRQKIEFAQLY 427

Query: 257 ----------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG 284
                      + GFQVSKYMPFGP+++V+PYLLRRAEENRG
Sbjct: 428 GMAESLSFGLRNAGFQVSKYMPFGPIELVLPYLLRRAEENRG 469

[Close]

BLAST of Lsi03G002120 vs. TAIR10
Match: AT5G38710.1 (AT5G38710.1 Methylenetetrahydrofolate reductase family protein)

HSP 1 Score: 315.8 bits (808), Expect = 3.0e-86
Identity = 165/299 (55.18%), Postives = 208/299 (69.57%), Query Frame = 1


Query: 15  HASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPK 74
           H S VV K++AICP+ +L+RVSDLLRWQ KNP+F LPWKL + P+FS  SPLYHT  +P+
Sbjct: 168 HLSSVVVKITAICPMNVLKRVSDLLRWQYKNPNFKLPWKLNSFPVFSGLSPLYHTTSEPE 227

Query: 75  PLTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHN 134
           PLT EEE+ L+ +HERL  +C  C ++N+PL IDAE T +QPAIDY  Y +AI+ N D +
Sbjct: 228 PLTVEEERELEKAHERLKSVCLRCQESNVPLLIDAEDTILQPAIDYMAYWSAIMFNSDKD 287

Query: 135 -PIVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIH 194
            PIVY TIQAYLKDA +RL L  +E+ KM VP+G KLVRGAYMSSE+KLA SLG++SP+H
Sbjct: 288 RPIVYNTIQAYLKDAGERLHLALRESEKMNVPIGFKLVRGAYMSSEAKLADSLGYKSPVH 347

Query: 195 NTIQDTHFCYNTCASFLLDNIAKGSS-GAILATHNIESGNELCSMLYALLISKP------ 254
           +TIQ+TH CYN C SFL++  + GS    ILATHN +SG         L I+K       
Sbjct: 348 DTIQNTHDCYNDCMSFLMEKASNGSGIAVILATHNTDSGKLGARKASELGINKENGKIEF 407

Query: 255 TQLFD---------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
            QL+             GF VSKYMP+GPVD  +PYL+RRA ENRG   +G L R++ R
Sbjct: 408 AQLYGMSDALSFGLKRAGFNVSKYMPYGPVDTAIPYLIRRAYENRGMMSTGALDRQLMR 466

[Close]

BLAST of Lsi03G002120 vs. TAIR10
Match: AT3G30775.1 (AT3G30775.1 Methylenetetrahydrofolate reductase family protein)

HSP 1 Score: 307.4 bits (786), Expect = 1.1e-83
Identity = 162/299 (54.18%), Postives = 205/299 (68.56%), Query Frame = 1


Query: 15  HASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPK 74
           H S VV K++AICP+ LL+RVSDLLRW+ K+P+F L WKL++ P+FSESSPLYHT  +P+
Sbjct: 189 HFSSVVVKITAICPISLLKRVSDLLRWEYKSPNFKLSWKLKSFPVFSESSPLYHTNSEPE 248

Query: 75  PLTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHN 134
           PLT EEE+ L+ +H R+ +IC  C ++N+PL IDAE T +QPAIDY  YS+AI+ N D +
Sbjct: 249 PLTAEEERELEAAHGRIQEICRKCQESNVPLLIDAEDTILQPAIDYMAYSSAIMFNADKD 308

Query: 135 -PIVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIH 194
            PIVY TIQAYL+DA +RL L  + A K  VP+G KLVRGAYMSSE+ LA SLG +SP+H
Sbjct: 309 RPIVYNTIQAYLRDAGERLHLAVQNAEKENVPMGFKLVRGAYMSSEASLADSLGCKSPVH 368

Query: 195 NTIQDTHFCYNTCASFLLDNIAKGSS-GAILATHNIESGNELCSMLYALLISKP------ 254
           +TIQDTH CYN C +FL++  + GS  G +LATHN +SG         L I K       
Sbjct: 369 DTIQDTHSCYNDCMTFLMEKASNGSGFGVVLATHNADSGRLASRKASDLGIDKQNGKIEF 428

Query: 255 TQLFD---------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
            QL+             GF VSKYMPFGPV   +PYLLRRA ENRG   +G   R++ R
Sbjct: 429 AQLYGMSDALSFGLKRAGFNVSKYMPFGPVATAIPYLLRRAYENRGMMATGAHDRQLMR 487

[Close]

BLAST of Lsi03G002120 vs. NCBI nr
Match: gi|778707546|ref|XP_004141603.2| (PREDICTED: proline dehydrogenase 2, mitochondrial-like [Cucumis sativus])

HSP 1 Score: 464.5 bits (1194), Expect = 1.5e-127
Identity = 239/296 (80.74%), Postives = 254/296 (85.81%), Query Frame = 1


Query: 16  ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKP 75
           ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKL+TLPIFSESSPLYHTLEQP+P
Sbjct: 185 ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLEQPEP 244

Query: 76  LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNP 135
           LT EEEKSLQMSHERLMKIC+SCVDAN+PLAIDAEHTKVQPAIDYFTYSAAIIHNKD NP
Sbjct: 245 LTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKDRNP 304

Query: 136 IVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNT 195
           IVYGTIQAYLKDAKDRLLL  KEA+K+KVPLGIKLVRGAYMSSESKLASSLGFESPIH+T
Sbjct: 305 IVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGFESPIHDT 364

Query: 196 IQDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFD---- 255
           IQDTH CYNTCASFLLD+IAKGS+GAILATHN+ESG    S  Y + I K  Q  +    
Sbjct: 365 IQDTHSCYNTCASFLLDDIAKGSTGAILATHNVESGKLAASRAYEIGIGKLKQKLEFAQL 424

Query: 256 -----------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
                       + GFQVSKYMPFGPVDMVMPYLLRRAEENRG   +  L RE+ R
Sbjct: 425 YGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRELMR 480

[Close]

BLAST of Lsi03G002120 vs. NCBI nr
Match: gi|659119046|ref|XP_008459446.1| (PREDICTED: proline dehydrogenase 2, mitochondrial [Cucumis melo])

HSP 1 Score: 463.4 bits (1191), Expect = 3.3e-127
Identity = 237/296 (80.07%), Postives = 254/296 (85.81%), Query Frame = 1


Query: 16  ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKP 75
           ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKL+TLPIFSESSPLYHTLEQP+P
Sbjct: 179 ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLEQPEP 238

Query: 76  LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNP 135
           LT EEEKSLQ+SHERLMKIC+SCVDAN+PLAIDAEHTKVQPAIDYFTYSAAIIHNKD NP
Sbjct: 239 LTREEEKSLQISHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKDRNP 298

Query: 136 IVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNT 195
           IVYGTIQAYLKDAKDRLLL  KEA+K+K+PLGIKLVRGAYMSSESKLASSLGFESPIHNT
Sbjct: 299 IVYGTIQAYLKDAKDRLLLANKEASKLKIPLGIKLVRGAYMSSESKLASSLGFESPIHNT 358

Query: 196 IQDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFD---- 255
           IQDTH CYNTCASFLLD+IAKGSSGAILATHN+ESG    +  Y + I K  Q  +    
Sbjct: 359 IQDTHSCYNTCASFLLDDIAKGSSGAILATHNVESGKLAATRAYEIGIGKLKQKLEFAQL 418

Query: 256 -----------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
                       + GFQVSKYMPFGPVDMVMPYLLRRAEENRG   +  L R++ R
Sbjct: 419 YGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLMR 474

[Close]

BLAST of Lsi03G002120 vs. NCBI nr
Match: gi|1009127021|ref|XP_015880472.1| (PREDICTED: proline dehydrogenase 2, mitochondrial-like [Ziziphus jujuba])

HSP 1 Score: 383.3 bits (983), Expect = 4.3e-103
Identity = 190/295 (64.41%), Postives = 229/295 (77.63%), Query Frame = 1


Query: 17  SFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKPL 76
           SFVV K+SAICPLRLLERVSDLLRWQ KNPS +LPWKL T PIFS+SSP YHTL +P+PL
Sbjct: 203 SFVVVKISAICPLRLLERVSDLLRWQHKNPSCSLPWKLNTFPIFSDSSPTYHTLTEPQPL 262

Query: 77  TNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNPI 136
           T EEE+ LQ++H+RL+ +C+ C++AN+PL +DAEHT VQPAIDY TYS+AI++NKD+NPI
Sbjct: 263 TPEEERDLQLAHQRLLTLCQECLEANLPLLVDAEHTAVQPAIDYLTYSSAIMYNKDNNPI 322

Query: 137 VYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNTI 196
           VYGT+QAYLKDA DRL L TK A KM VPLG+KLVRGAYMSSE +LASSLG+ESPIHNTI
Sbjct: 323 VYGTLQAYLKDAGDRLFLATKAAEKMGVPLGVKLVRGAYMSSERELASSLGYESPIHNTI 382

Query: 197 QDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFD----- 256
           QDTH  +N CASF+L+ +A GS   +LATHN++SG    +  + L I +  Q  +     
Sbjct: 383 QDTHSSFNDCASFMLERLANGSDALVLATHNVQSGKLAAAKAHDLGIGRVNQKLEFAQLY 442

Query: 257 ----------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
                      + GFQVSKYMPFGP+D+VMPYLLRRAEENRG   S  L RE+ R
Sbjct: 443 GMADSLSYGLGNAGFQVSKYMPFGPIDVVMPYLLRRAEENRGMLSSSTLDRELMR 497

[Close]

BLAST of Lsi03G002120 vs. NCBI nr
Match: gi|442022546|gb|AGC51786.1| (proline oxidase [Manihot esculenta])

HSP 1 Score: 378.3 bits (970), Expect = 1.4e-101
Identity = 191/295 (64.75%), Postives = 227/295 (76.95%), Query Frame = 1


Query: 17  SFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKPL 76
           S VV K++AICPL LLERV+DLLRWQ+K+PSFNLPWK  T PIFSES+PLYHTL++P+PL
Sbjct: 190 SAVVVKITAICPLNLLERVADLLRWQEKDPSFNLPWKQNTFPIFSESTPLYHTLKKPEPL 249

Query: 77  TNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNPI 136
           T EEE+ L +  ++L+K+C++C +AN+PL IDAEHTKVQPAIDYFTYS+AI +NKD NPI
Sbjct: 250 TPEEEQHLLLGQQKLLKLCQACAEANVPLVIDAEHTKVQPAIDYFTYSSAIKYNKDDNPI 309

Query: 137 VYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNTI 196
           VYGTIQAYLKDAK+RLLL TK A KM +P+G KLVRGAYMSSE KLASSLG++SPIHN+I
Sbjct: 310 VYGTIQAYLKDAKERLLLATKAADKMGLPMGFKLVRGAYMSSERKLASSLGYQSPIHNSI 369

Query: 197 QDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFD----- 256
           Q TH CYN CASFLL+ IA  S G ILATHN+ESG         L I    Q  +     
Sbjct: 370 QQTHDCYNDCASFLLEAIADRSHGLILATHNVESGTLAAKKAEELGIGNGNQRLEFAQLY 429

Query: 257 ----------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
                      + GFQVSKY+PFGPV+MV+PYLLRRAEENRG   + GL RE+ R
Sbjct: 430 GMAEALSFGLRNAGFQVSKYLPFGPVEMVIPYLLRRAEENRGLLSASGLDRELTR 484

[Close]

BLAST of Lsi03G002120 vs. NCBI nr
Match: gi|1000961151|ref|XP_002521220.2| (PREDICTED: proline dehydrogenase 2, mitochondrial, partial [Ricinus communis])

HSP 1 Score: 377.5 bits (968), Expect = 2.4e-101
Identity = 190/293 (64.85%), Postives = 221/293 (75.43%), Query Frame = 1


Query: 17  SFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKPL 76
           SFVV K++AIC L+LLERVSDLLRWQQ +PSFNLPWK  TLPIFSE+SPLYHTLE+P+ L
Sbjct: 48  SFVVVKMTAICTLKLLERVSDLLRWQQTDPSFNLPWKQNTLPIFSEASPLYHTLEKPESL 107

Query: 77  TNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNPI 136
           T EEE  LQ+  +RL+++C+ CVDAN+PL +DAEHT +QPAIDYFTYS+AI+HNKD NP+
Sbjct: 108 TPEEECELQLGQQRLLRLCQECVDANVPLVVDAEHTAIQPAIDYFTYSSAIMHNKDDNPV 167

Query: 137 VYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNTI 196
           VY TIQAYLKDAK RLLL TK A KM VP+G KLVRGAYMSSE KLAS+LG++SPIHNTI
Sbjct: 168 VYNTIQAYLKDAKQRLLLATKAADKMGVPMGFKLVRGAYMSSERKLASALGYDSPIHNTI 227

Query: 197 QDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFD----- 256
           Q TH CYN CASFLLD I   S G ILATHN+ESG         L I    Q  +     
Sbjct: 228 QQTHSCYNDCASFLLDTITNSSHGIILATHNVESGRLAAKKARELGIRNGDQKLEFAQLY 287

Query: 257 ----------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREI 292
                      + GFQVSKY+PFGP+D V+PYLLRRAEENRG   +  L RE+
Sbjct: 288 GMSEALSFGLRNAGFQVSKYIPFGPIDTVIPYLLRRAEENRGLLSASNLDREL 340

The following BLAST results are available for this feature:

BLAST of Lsi03G002120 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PROD2_ARATH	5.3e-85	55.18	Proline dehydrogenase 2, mitochondrial OS=Arabidopsis thaliana GN=POX2 PE=2 SV=1	[more]
PROD1_ARATH	1.9e-82	54.18	Proline dehydrogenase 1, mitochondrial OS=Arabidopsis thaliana GN=POX1 PE=2 SV=2	[more]
PROD_DICDI	2.6e-31	33.56	Proline dehydrogenase 1, mitochondrial OS=Dictyostelium discoideum GN=prodh PE=3...	[more]
PROD_CAEEL	1.2e-23	29.50	Proline dehydrogenase 1, mitochondrial OS=Caenorhabditis elegans GN=B0513.5 PE=3...	[more]
PROD_DROME	1.9e-21	28.82	Proline dehydrogenase 1, mitochondrial OS=Drosophila melanogaster GN=slgA PE=2 S...	[more]

BLAST of Lsi03G002120 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KXN4_CUCSA	1.0e-127	80.74	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G643300 PE=4 SV=1	[more]
V9M5A2_MANES	9.7e-102	64.75	Proline oxidase OS=Manihot esculenta GN=ProDH PE=2 SV=1	[more]
A0A151RH61_CAJCA	2.8e-101	63.39	Uncharacterized protein OS=Cajanus cajan GN=KK1_036702 PE=4 SV=1	[more]
A0A0B2Q656_GLYSO	3.7e-101	63.39	Proline dehydrogenase 2, mitochondrial OS=Glycine soja GN=glysoja_046995 PE=4 SV...	[more]
M5X4N6_PRUPE	1.1e-100	62.41	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004722mg PE=4 SV=1	[more]

BLAST of Lsi03G002120 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G38710.1	3.0e-86	55.18	Methylenetetrahydrofolate reductase family protein	[more]
AT3G30775.1	1.1e-83	54.18	Methylenetetrahydrofolate reductase family protein	[more]

BLAST of Lsi03G002120 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778707546\|ref\|XP_004141603.2\|	1.5e-127	80.74	PREDICTED: proline dehydrogenase 2, mitochondrial-like [Cucumis sativus]	[more]
gi\|659119046\|ref\|XP_008459446.1\|	3.3e-127	80.07	PREDICTED: proline dehydrogenase 2, mitochondrial [Cucumis melo]	[more]
gi\|1009127021\|ref\|XP_015880472.1\|	4.3e-103	64.41	PREDICTED: proline dehydrogenase 2, mitochondrial-like [Ziziphus jujuba]	[more]
gi\|442022546\|gb\|AGC51786.1\|	1.4e-101	64.75	proline oxidase [Manihot esculenta]	[more]
gi\|1000961151\|ref\|XP_002521220.2\|	2.4e-101	64.85	PREDICTED: proline dehydrogenase 2, mitochondrial, partial [Ricinus communis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0006562	proline catabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0004657	proline dehydrogenase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR015659	Proline_oxidase
IPR002872	Proline_DH_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006525	arginine metabolic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0006561	proline biosynthetic process
biological_process	GO:0006562	proline catabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004657	proline dehydrogenase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G002120.1	Lsi03G002120.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002872	Proline dehydrogenase domain	PFAM	PF01619	Pro_dh	coord: 33..283 score: 5.8
IPR015659	Proline oxidase family	PANTHER	PTHR13914	PROLINE OXIDASE	coord: 17..283 score: 3.0E
None	No IPR available	PANTHER	PTHR13914:SF0	PROLINE DEHYDROGENASE 1, MITOCHONDRIAL	coord: 17..283 score: 3.0E

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi03G002120	Cucumber (Chinese Long) v3	cuclsiB378
Lsi03G002120	Cucumber (Chinese Long) v3	cuclsiB384
Lsi03G002120	Watermelon (97103) v2	lsiwmbB222
Lsi03G002120	Watermelon (97103) v2	lsiwmbB238
Lsi03G002120	Wax gourd	lsiwgoB310
Lsi03G002120	Wax gourd	lsiwgoB342
Lsi03G002120	Cucumber (Gy14) v1	cgylsiB387
Lsi03G002120	Cucumber (Gy14) v1	cgylsiB464
Lsi03G002120	Cucurbita maxima (Rimu)	cmalsiB276
Lsi03G002120	Cucurbita maxima (Rimu)	cmalsiB656
Lsi03G002120	Cucurbita moschata (Rifu)	cmolsiB265
Lsi03G002120	Cucurbita moschata (Rifu)	cmolsiB363
Lsi03G002120	Cucurbita moschata (Rifu)	cmolsiB645
Lsi03G002120	Cucurbita pepo (Zucchini)	cpelsiB143
Lsi03G002120	Cucurbita pepo (Zucchini)	cpelsiB339
Lsi03G002120	Wild cucumber (PI 183967)	cpilsiB357
Lsi03G002120	Wild cucumber (PI 183967)	cpilsiB362
Lsi03G002120	Cucumber (Chinese Long) v2	culsiB353
Lsi03G002120	Cucumber (Chinese Long) v2	culsiB359
Lsi03G002120	Melon (DHL92) v3.5.1	lsimeB232
Lsi03G002120	Melon (DHL92) v3.5.1	lsimeB233
Lsi03G002120	Melon (DHL92) v3.5.1	lsimeB238
Lsi03G002120	Watermelon (Charleston Gray)	lsiwcgB245
Lsi03G002120	Watermelon (Charleston Gray)	lsiwcgB226
Lsi03G002120	Watermelon (97103) v1	lsiwmB258
Lsi03G002120	Watermelon (97103) v1	lsiwmB278
Lsi03G002120	Cucumber (Gy14) v2	cgyblsiB325
Lsi03G002120	Melon (DHL92) v3.6.1	lsimedB242
Lsi03G002120	Melon (DHL92) v3.6.1	lsimedB243
Lsi03G002120	Melon (DHL92) v3.6.1	lsimedB249
Lsi03G002120	Silver-seed gourd	carlsiB017
Lsi03G002120	Silver-seed gourd	carlsiB236