Lsi03G000300 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi03G000300
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Carbonate dehydratase/ zinc ion binding protein
Location	chr03 : 514408 .. 520615 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTCTCTAGAGATTGGAACATGGGGAAGCTCTTGTGCTCTTTCTTCTTTTCCATTCTTTTGCTTTCATTTAAGGCAATATCCCAAGAAGTTGGTGAGACTCCTAAATTATGAAATGATTTTTTTTTGTTTGAAACGTTTTAATTATTTATGGTGCGTTTGGATTGACTTTCTAAGTGTTTATAAGTGCAAAAAAACGTGTTTATAAACACTTAGAAAGTCAATCCAAACATGCCCTTAGTCTCTGTTGAGATTCCCGCACTAGAAAAATCAAAGGCACCTCATAATGTTTATAAGATACATGTGTTACTCCTCTTATTACTAATTAATTTTGAGATAGAACTATATGTTATCTAATATGGTAACGAAGTCTATGAAGCCTAAACGGACATTTCGTCCATAATGGTGAACTCAAAGAGGCCTCATCTTGAGGGATATATATTGGTGTATCCCAAATTGAAAAAAAAGTTAAGAGATCTCACAATCTTTCGAAAGATACACGAGCTACTTCTCTTATTGTCAATTGATTTTGAGATGAAACTCCCTATTATCTAATAGTTTCACACGTTTTAACATTTTTCAATTATACTCTGTATCTTAGTGAATGTTAAATGTTGTTGATAATATGAGTACGTAGTAAATGAGAATAATACTTATGGTGTATTTGAACTGACTTTCTAAGTGTTTAAATAAGTGTTTATAAGTGTAAAAAAATATTTATAAACACTTTGAAAGTCATTTTAAACTGGCTAATAGTGAATTGGTATAAAATTTGGCCTTCAAAATATATATATATATATATATATATATATATATTACAAACTGTATTATTATTATTATCATCATCATCATTTATTATTGGGTTTAAGGATTATGATATTGTTTATGCAGATGATGAAAGAGGGTTTGATTATGAAAGAGATGGGGAAAAAGGACCAATTCATTGGGGAGAGCTTAGACCAGAGTGGCATACATGTAAGGCTGGGTTAATGCAATCTCCAATTGATTTATTGCATGAGAGAGTTGAAGTTATTTCACACTTGGGAGACCTTAAAATCAACTACAAATCCTCTAATGCCACACTCAAGAATAGAGGCCATGATATGATGGTAATTATATTTTCTTTCCCTTTTTAGAATGATTAAAAGTGCTTTTTGTTATATATAAAATCACTCTCTAACATATTTTTAATCATTCAAAACTACACAAATTACATATAGAAGTATATTAAATCAAATATGTTAAATTGATTTTGAATAATTAAAGACGTGTCCCTGAGTAATTTTAAATAAGGAAAAAAAACCTTTTTCGATCTCTAGGTTTTAGACCTAGTTTCCATTTGGTCCCTATGTTTCAAAATAGTACACTCTTAATTCTTAAGTTTTGATTTTGATTTCAATTTAGACTTTTTAAGTTTCAAAATGTTATAAATTTACCTCTGAGATCTAAATTTTGTTTTAATTTGATCTCTAAGTTTGAAAACTTACACTTTTAACCTCAGTTTGATCTCTAGGTTTGTTATAAAAATATTTTTTAAAAAAGTCATTTTTATTTAAACTATTTTGATTAAAACGGTTTAAAATACACTTTAAAAGTGTTTCAAAAGCTATATTGAGAGGTTGTCAAACACTTCAATTTTTTCTAAAATGACTTTATCTTCAAAATTAAACATTTTCAAATTAAACCAAACACACCCTTACACTTTTTAGTAACATTAATTATTCACTAAATACTCACTTTAATCTTTAGCATTAATGTCTATTCATTAATTTAAAATAATTAAAATAAAAATAATTTTAATCATTTTAAAATCGCTAGCTTATAAGCGAAACTATTTTGATTATATAGATGAAATGGGTGGATGGAGGTGGATTCATGGAAATGAATGGAACTCAATATAAACTAAAACAATGTCATTGGCACTCACCTTCCGAGCACACCATTGATGGCAAGAAGTTTGATCTTGAAGCTCACTTAGTCCATGAAAGCTCAAACGGCATGATTTCTGTCATCGGAATCTTGTATCAGATTGGAGAACCTGATTACTTTTTATCAATGGTGAGTTTAATGTATAACCGAGTTGATCTGTTGCATAAACAAGTTGAATTTTGTTTCATGTTTTTAAATTCATCGAGTTTAACAAATATGGATTCGACAAATGTATTTTTTTTAAAACTACTCTTAGATTATTCTATGATCTAAATAGTGCAGGTTCTAGAACCAATTTTTATGTTTGATTTTGAATTTTTGAAGAATTATATTAAAATAGATAAAAATTACTTGAATTAAATATAGTATTTTGATGTAATAAAATACTCAATTCATTTTTTTTTGTCTAATTAAAATGTGAATAATATAATATATTATAAATTATATGATATTATATTGTAAAATAATAATTGTACAACTGTGTTTTAGGGTAATTTTGTTTTAAATGACAAAGCTATTGAAAATATTTTTAAATATAGCAAATGTTTATCAATGATGGACAGTGATACACATCTATCATCTATCACTTGTCTATTAGTGATAGACAGTGATACACATCTATCATCTATCAATGTCTATCAGTGATAGACATTAATAGATGTTTATCAATGTTTATTACTGTCTATCACTGATAGATAGTGACATTTTAAATAAGTTAGCTCATTTTGTTATATTTGAAAACAACCCATTTTTTTATTATTATAAAACACAAATTAATTAACAATATTTTATATTAAACTTAATATCTAGTGGATAACCACAACCAATTTTATGATTTATAAATATATATTACATCAAATACATTTTGAACTATTGCTTAAATGAGATTCATTTTCTGAACATGCTATCGAACATAAACTCAAAAATATGAAATACAACTGTGCTTTCATTCAATTCTTTGTTTTCAGATTTATGTTTTCGGGTATGGACTTACCAACAAATCCTAATATTGTGGGAAAATTTGAATTCACATTGTTTCAATCTCATTGTATCAGATTGAAGATCATTTAGAACTGGCCTCAGATACCCATGAAGAAGAGAGAGTGATTGGAATAATTGATCCAAATCTGTTGAAGATGGGTAGCAAACAATATTATAGATACATAGGCTCACTTACTGTTCCACCATGCACTGAAAATGTGATGTGGACTATGATTCACCAGGTACATTTCTTCAATACTTAGTATGTTATATTATTAATATGTTTTTAAATATAGCAAAATGAGTTAAAATATTTATAAATATAGCAAAATGTCATTGTCTATCACTAATAGAAACTGATAGCATTTATCATGGTCTATCATTGTCTACACTGATAGACAGTGATATTTTGCTATATTTGTAAATATTTCCAGCAGTTTTGCCATTTAAAACAATTACTTATATATATAATTCAAATAGTTTATGTTTTTTTTTTTTTAGAGTATGGAGATTGTGGATTTAAATCTATAATTTTCCTGATCGAAGATATATATTGTATGCCACTTGCATGTCTTTGAGTCTTTTTGTAAATCAAAATTGATATTTTTGTATTATTTGAGTGTGCTAAAAATTAAATTGAGAATATCCTCTCTTTTGTTTTTACTTTTTATACTCTTTTAAAAGATAGATCTTGTTTGATAACTATTTGACTTTTTATTTTTGATTTTGAAAATTAAGCCTATAAATTATACTTTTCACCTCTAAATTCTTATGTTATATTGTGCACATATTACAAATATTTTCATAAACCAACAAGTAATTTTTAAAAATTGGTTTTTGTTTTTCGATTTTAGACTAAGAATTCTAATGTTTCTTTAATAAAAGTAAAAATAATGAAAAAAAAATTGAGAGAAAACAAGTATAAATTTCAAAAACTAAAAACTAACAAAATGATAACCAAAGCATGACAAAACTCTTAGATTTGTGTCTATTTGGGTCCGCTTTTAGAATTATGTAATATGTATCTAAACTTTCAATTTTTATGAATATTAATAAGTCATTAATCTTTACATAGAGTGTCTAATAGATATAAAATTGAATTTTATGTCTCGCTTAAATTTTATGTCTAGTAGATATGTGAATTTTAAAAAAAAATGTCAAATCGGTCAAAATCCTATTTAGATATAAAATTAAAATTTTAGGATCTATTGTACAATACAAAAATGAAAGTTTAAAAATTTACTATAGACAATTTTTAAAGTTGAAGAACTTAGTGGACATAAACTTAAAAATTTAAGAATCTATAAGACGTTTTTGAAAGTTTAATGACTTATTAAATACAAATTAATAATTTAAGGAGCTATTCTAACACTTTTTGAAAGTCTACTAACTGAAGAAATAAATTGAGAAGCTCAAGAATTAAACTTCTCATTTAACTTAAAATTAGATAAACTTTTTTGGCTTAAAATATCTTTAGTTTGGCTAAAAACATTACTCCTATTTTTTTTTAAGAGCCAAAAGTAGTTTTTTTTTCTAATTTTTTTTAATTTTTTTAATTTTTTTTAATTTTTTTTTAATTTTTATTTTACAAGAAAGGGTGAGAATTAAACAAAACATAGAAATTGAAGGAAAGAAAAAGCCTTTTTTAAAAATAAAATAGTAACCAAATAAAATTTAAATTTAATTACCTAATTGCTTAAACTATAAAACATATGTTATATTTATACGATGCATGCGTAGATTATTAATTTCAATTACTAATTTTAATCTTGATTTCAATAGGTGAGGACTGTTACAGAAGAACAAGTGAAATTACTTCGAGTAGCTGTCCATGATGTAAGTTAATTATAATAATTAATTGAGATGCCAAGATATTTAATTTTTTATATATATTATAATAAATGAAATTTTAGAAAACATATATAATAAATTATTTTTAATATAACAAGGTAGAGAAATTTGATCCAGAGACAAATTTAATTAAGGTTGGAGGCTCAAGCTCCCTCAATTTTTTGAGTTGTTTTCAAATATAGCAAAATGAGCCAACTTATTTACAAATATAGCAAAATGTCATTGTCTATTAGTGATAGACAATAACATTTTACTATATTTAAAAATATTTTTCAGCACTTTTGTCATTTAAAATAATTATTCATTTTTAAAAATTTATATATACTTTTTTTAAAAAAAAAAAATTTAAAACACAAAAAAAACCCAGGAACTTCAATGGTTTGACTGACTTCAACCCCCTCGACATCTCAAGTTCGAATCCCATATGTGTACAATCTTATGAAATCTTCTCCAATTTCATTGAAAATAGTACATCTCAATTACTAATATATTAATGTGATGATTAATAGTGAAAGCACTATCATATAATTAAAACATATAATTGATGATTGTTTAAGGTTCTGTTTAGTAATTATTTGACTTTTCGTTTTTTTGTTTTTAAAAATTAACAAAAACTACTTTCTTACCTACGATGATTTCTTACCATGATTTGTATCTTTCTTGAGTACAAGAGTTTAATTTCTAGCGAAATTTTAAAAACAAAAACAAATTTTTAAAAGGGGACTTTTTAAAAATAGAAAAAAAAAAAAAAAACTATTTACACAAAATAACAAAATTTTTAGATAGTTGGATAGATGATGATAGAAGTCTATCAAGGTCTATCGGTGATAGAAATGATAGAACTCTATCACTAATAGATGCTGATAGAAGTCTATCAATGTCTATCAGTGATTTTTTACTATTTTTTATAAATAGTTTGACATTTTTTCTATTTGTGAAAATTTTCCTTTTTAAAAACTATTTTTTTAGTTTTCAAAATTTAATTTCGTTTTCAAAAAATTGGTAGAAAGCAGATAACAAATCAAAAAATTTAGAGGTGGAAGAGGCATGTTTATAGGTTTAATTTTTAAAAACTAAAAATCGAAAAATTAAATTGTTATCAATCAAAACGTGTGACCTAAAATATTTAAATAAAATTGAAATTGAAATTAAAGACTGAAATTTTATTTTGAGACGTTTGTTAATCAAAGGTTTGATATGTGAAAGCAGGACTCGGATTCTAATGCAAGACCGTTGCAACTAATCTATAATCGAACAATGCAACTCCAAAGATCGAAAGAAAGTGAAGAATGAATTTATATATTTATTTACATTATGATGCAGGCCGTTTTTCTCACAGTGATGTATCCATTTTCCAATAATACTATCCCAGTTTTCATATTTTGTATTTCTTATTGATTATTAGGGATAAAAATACCATATATTTATTATATATTTCTAAAATTCAGG

mRNA sequence

ATTCTCTAGAGATTGGAACATGGGGAAGCTCTTGTGCTCTTTCTTCTTTTCCATTCTTTTGCTTTCATTTAAGGCAATATCCCAAGAAGTTGATGATGAAAGAGGGTTTGATTATGAAAGAGATGGGGAAAAAGGACCAATTCATTGGGGAGAGCTTAGACCAGAGTGGCATACATGTAAGGCTGGGTTAATGCAATCTCCAATTGATTTATTGCATGAGAGAGTTGAAGTTATTTCACACTTGGGAGACCTTAAAATCAACTACAAATCCTCTAATGCCACACTCAAGAATAGAGGCCATGATATGATGATGAAATGGGTGGATGGAGGTGGATTCATGGAAATGAATGGAACTCAATATAAACTAAAACAATGTCATTGGCACTCACCTTCCGAGCACACCATTGATGGCAAGAAGTTTGATCTTGAAGCTCACTTAGTCCATGAAAGCTCAAACGGCATGATTTCTGTCATCGGAATCTTGTATCAGATTGGAGAACCTGATTACTTTTTATCAATGATTGAAGATCATTTAGAACTGGCCTCAGATACCCATGAAGAAGAGAGAGTGATTGGAATAATTGATCCAAATCTGTTGAAGATGGGTAGCAAACAATATTATAGATACATAGGCTCACTTACTGTTCCACCATGCACTGAAAATGTGATGTGGACTATGATTCACCAGGTGAGGACTGTTACAGAAGAACAAGTGAAATTACTTCGAGTAGCTGTCCATGATGACTCGGATTCTAATGCAAGACCGTTGCAACTAATCTATAATCGAACAATGCAACTCCAAAGATCGAAAGAAAGTGAAGAATGAATTTATATATTTATTTACATTATGATGCAGGCCGTTTTTCTCACAGTGATGTATCCATTTTCCAATAATACTATCCCAGTTTTCATATTTTGTATTTCTTATTGATTATTAGGGATAAAAATACCATATATTTATTATATATTTCTAAAATTCAGG

Coding sequence (CDS)

ATGGGGAAGCTCTTGTGCTCTTTCTTCTTTTCCATTCTTTTGCTTTCATTTAAGGCAATATCCCAAGAAGTTGATGATGAAAGAGGGTTTGATTATGAAAGAGATGGGGAAAAAGGACCAATTCATTGGGGAGAGCTTAGACCAGAGTGGCATACATGTAAGGCTGGGTTAATGCAATCTCCAATTGATTTATTGCATGAGAGAGTTGAAGTTATTTCACACTTGGGAGACCTTAAAATCAACTACAAATCCTCTAATGCCACACTCAAGAATAGAGGCCATGATATGATGATGAAATGGGTGGATGGAGGTGGATTCATGGAAATGAATGGAACTCAATATAAACTAAAACAATGTCATTGGCACTCACCTTCCGAGCACACCATTGATGGCAAGAAGTTTGATCTTGAAGCTCACTTAGTCCATGAAAGCTCAAACGGCATGATTTCTGTCATCGGAATCTTGTATCAGATTGGAGAACCTGATTACTTTTTATCAATGATTGAAGATCATTTAGAACTGGCCTCAGATACCCATGAAGAAGAGAGAGTGATTGGAATAATTGATCCAAATCTGTTGAAGATGGGTAGCAAACAATATTATAGATACATAGGCTCACTTACTGTTCCACCATGCACTGAAAATGTGATGTGGACTATGATTCACCAGGTGAGGACTGTTACAGAAGAACAAGTGAAATTACTTCGAGTAGCTGTCCATGATGACTCGGATTCTAATGCAAGACCGTTGCAACTAATCTATAATCGAACAATGCAACTCCAAAGATCGAAAGAAAGTGAAGAATGA

Protein sequence

MGKLLCSFFFSILLLSFKAISQEVDDERGFDYERDGEKGPIHWGELRPEWHTCKAGLMQSPIDLLHERVEVISHLGDLKINYKSSNATLKNRGHDMMMKWVDGGGFMEMNGTQYKLKQCHWHSPSEHTIDGKKFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSMIEDHLELASDTHEEERVIGIIDPNLLKMGSKQYYRYIGSLTVPPCTENVMWTMIHQVRTVTEEQVKLLRVAVHDDSDSNARPLQLIYNRTMQLQRSKESEE

Homology

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BLAST of Lsi03G000300 vs. Swiss-Prot
Match: ATCA7_ARATH (Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana GN=ACA7 PE=2 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 7.8e-82
Identity = 144/264 (54.55%), Postives = 197/264 (74.62%), Query Frame = 1


Query: 6   CSFF------FSILLLSFKAISQ-EVDDERGFDYERDGEKGPIHWGELRPEWHTCKAGLM 65
           C FF      F+I+ +S  A S  EV+DER F+Y+++ EKGP  WGEL+PEW  C  G M
Sbjct: 9   CIFFVALFSIFTIVSISSAASSHGEVEDEREFNYKKNDEKGPERWGELKPEWEMCGKGEM 68

Query: 66  QSPIDLLHERVEVISHLGDLKINYKSSNATLKNRGHDMMMKWVDGGGFMEMNGTQYKLKQ 125
           QSPIDL++ERV ++SHLG L  +Y  SNATLKNRGHD+M+K+ DG G +++NG +Y+L+Q
Sbjct: 69  QSPIDLMNERVNIVSHLGRLNRDYNPSNATLKNRGHDIMLKFEDGAGTIKINGFEYELQQ 128

Query: 126 CHWHSPSEHTIDGKKFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSMIEDHLELASDT 185
            HWHSPSEHTI+G++F LE H+VHE  N  ++V+ +LY+IG  D F+  +E  LE  ++ 
Sbjct: 129 LHWHSPSEHTINGRRFALELHMVHEGRNRRMAVVTVLYKIGRADTFIRSLEKELEGIAEM 188

Query: 186 HEEERVIGIIDPNLLKMGSKQYYRYIGSLTVPPCTENVMWTMIHQVRTVTEEQVKLLRVA 245
            E E+ +G+IDP  +K+GS++YYRY GSLT PPCT+NV W+++ +VRTVT +QVKLLRVA
Sbjct: 189 EEAEKNVGMIDPTKIKIGSRKYYRYTGSLTTPPCTQNVTWSVVRKVRTVTRKQVKLLRVA 248

Query: 246 VHDDSDSNARPLQLIYNRTMQLQR 263
           VHDD++SNARP+Q    R + L R
Sbjct: 249 VHDDANSNARPVQPTNKRIVHLYR 272

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BLAST of Lsi03G000300 vs. Swiss-Prot
Match: NEC3_NICLS (Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3 OS=Nicotiana langsdorffii x Nicotiana sanderae GN=NEC3 PE=1 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 1.6e-74
Identity = 141/254 (55.51%), Postives = 187/254 (73.62%), Query Frame = 1


Query: 1   MGKLLCSFFFSILLLS--FKAISQEVDDERGFDYERDGEKGPIHWGELRPEWHTCKAGLM 60
           M  +    F S L LS  F A S EVDDE  F Y+   E GP +WG +RP+W  C +G +
Sbjct: 3   MAAITKMLFISFLFLSSVFLARSGEVDDESEFSYDEKSENGPANWGNIRPDWKEC-SGKL 62

Query: 61  QSPIDLLHERVEVISHLGDLKINYKSSNATLKNRGHDMMMKWVDGGGFMEMNGTQYKLKQ 120
           QSPID+   R EV+S+L  L+ +YK SNATL NRGHD+M++ +D GG++++N TQY+LKQ
Sbjct: 63  QSPIDIFDLRAEVVSNLRILQKDYKPSNATLLNRGHDIMLR-LDDGGYLKINETQYQLKQ 122

Query: 121 CHWHSPSEHTIDGKKFDLEAHLVHESSNGMISVIGILYQIGE-PDYFLSMIEDHLELASD 180
            HWH+PSEHTI+G++F+LEAHLVHES+NG   VIGI+Y+IG  PD FLSMIE+ L++ ++
Sbjct: 123 LHWHTPSEHTINGERFNLEAHLVHESNNGKFVVIGIVYEIGLWPDPFLSMIENDLKVPAN 182

Query: 181 THEEERVIGIIDPNLLKMGSKQYYRYIGSLTVPPCTENVMWTMIHQVRTVTEEQVKLLRV 240
               ER IGIIDPN +K+  K+Y+RYIGSLT PPCTE V+W +  +V+TVT  Q+KLL+ 
Sbjct: 183 KKGIERGIGIIDPNQIKLDGKKYFRYIGSLTTPPCTEGVVWIIDRKVKTVTRRQIKLLQE 242

Query: 241 AVHDDSDSNARPLQ 252
           AVHD  ++NARP Q
Sbjct: 243 AVHDGFETNARPTQ 254

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BLAST of Lsi03G000300 vs. Swiss-Prot
Match: ATCA5_ARATH (Alpha carbonic anhydrase 5 OS=Arabidopsis thaliana GN=ACA5 PE=3 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 3.9e-73
Identity = 133/262 (50.76%), Postives = 183/262 (69.85%), Query Frame = 1


Query: 1   MGKLLCSFFFSILLLSFKAISQEVDDERGFDYERDGEKGPIHWGELRPEWHTCKAGLMQS 60
           +G +    F SI ++S      EV+DE  F+YE+ GEKGP +WG L+PEW  C  G MQS
Sbjct: 6   IGYVFFLIFISITIVSSSPDHGEVEDETQFNYEKKGEKGPENWGRLKPEWAMCGKGNMQS 65

Query: 61  PIDLLHERVEVISHLGDLKINYKSSNATLKNRGHDMMMKWVDG--GGFMEMNGTQYKLKQ 120
           PIDL  +RV +  +LG L+  Y  SNAT+KNRGHD+MMK+  G  G  + +NGT+YKL+Q
Sbjct: 66  PIDLTDKRVLIDHNLGYLRSQYLPSNATIKNRGHDIMMKFEGGNAGLGITINGTEYKLQQ 125

Query: 121 CHWHSPSEHTIDGKKFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSMIEDHLELASDT 180
            HWHSPSEHT++GK+F LE H+VH+S +G  +V+   Y++G+PDYFL  +E +L+  +DT
Sbjct: 126 IHWHSPSEHTLNGKRFVLEEHMVHQSKDGRNAVVAFFYKLGKPDYFLLTLERYLKRITDT 185

Query: 181 HEEERVIGIIDPNLLKMGSKQYYRYIGSLTVPPCTENVMWTMIHQVRTVTEEQVKLLRVA 240
           HE +  + ++ P      SK YYR+IGSLT PPC+ENV+WT+  ++RTVT +Q+ +LRV 
Sbjct: 186 HESQEFVEMVHPRTFGFESKHYYRFIGSLTTPPCSENVIWTISKEMRTVTLKQLIMLRVT 245

Query: 241 VHDDSDSNARPLQLIYNRTMQL 261
           VHD S+SNARPLQ    R + L
Sbjct: 246 VHDQSNSNARPLQRKNERPVAL 267

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BLAST of Lsi03G000300 vs. Swiss-Prot
Match: ATCA8_ARATH (Alpha carbonic anhydrase 8 OS=Arabidopsis thaliana GN=ACA8 PE=3 SV=3)

HSP 1 Score: 265.0 bits (676), Expect = 8.9e-70
Identity = 131/244 (53.69%), Postives = 165/244 (67.62%), Query Frame = 1


Query: 23  EVDDERGFDYERDGEKGPIHWGELRPEWHTCKAGLMQSPIDLLHERVEVISHLGDLKINY 82
           EV+DE  F YE  G KGP  WG L  EW  C  G MQSPIDL  + V V +  G L+  Y
Sbjct: 133 EVEDETEFSYETKGNKGPAKWGTLDAEWKMCGIGKMQSPIDLRDKNVVVSNKFGLLRSQY 192

Query: 83  KSSNATLKNRGHDMMMKWVDG--GGFMEMNGTQYKLKQCHWHSPSEHTIDGKKFDLEAHL 142
             SN T+KNRGHD+M+K+  G  G  + + GT+Y+L+Q HWHSPSEHTI+GK+F LE HL
Sbjct: 193 LPSNTTIKNRGHDIMLKFKGGNKGIGVTIRGTRYQLQQLHWHSPSEHTINGKRFALEEHL 252

Query: 143 VHESSNGMISVIGILYQIGEPDYFLSMIEDHLELASDTHEEERVIGIIDPNLLKMGSKQY 202
           VHES +   +V+  LY +G  D FL  +E  L+  +DTH  E  +GIIDP  L   SK Y
Sbjct: 253 VHESKDKRYAVVAFLYNLGASDPFLFSLEKQLKKITDTHASEEHVGIIDPKKLSFESKHY 312

Query: 203 YRYIGSLTVPPCTENVMWTMIHQVRTVTEEQVKLLRVAVHDDSDSNARPLQLIYNRTMQL 262
           YRY GSLT PPC+ENV+W++  ++RTV+ +QVKLLRVAVHD SDSNARPLQ +  R + L
Sbjct: 313 YRYSGSLTAPPCSENVIWSVSKEIRTVSSKQVKLLRVAVHDASDSNARPLQAVNKRKVYL 372

Query: 263 QRSK 265
            + K
Sbjct: 373 YKPK 376

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BLAST of Lsi03G000300 vs. Swiss-Prot
Match: ATCA2_ARATH (Alpha carbonic anhydrase 2 OS=Arabidopsis thaliana GN=ACA2 PE=2 SV=2)

HSP 1 Score: 258.5 bits (659), Expect = 8.3e-68
Identity = 117/224 (52.23%), Postives = 166/224 (74.11%), Query Frame = 1


Query: 4   LLCSFFFSILLLSFKAISQEVDDERGFDYERDGEKGPIHWGELRPEWHTCKAGLMQSPID 63
           +L SF  ++  LS     +EV+DE  F YE + E GP  WG+LRPEW  C  G MQSPID
Sbjct: 13  VLTSFVTTVSCLSAATDYREVEDEHEFSYEWNQENGPAKWGKLRPEWKMCGKGEMQSPID 72

Query: 64  LLHERVEVISHLGDLKINYKSSNATLKNRGHDMMMKW-VDGGGFMEMNGTQYKLKQCHWH 123
           L+++RV +++HL  L  +YK  NATLKNRGHDMM+K+  +G G + +NGT+YKL Q HWH
Sbjct: 73  LMNKRVRLVTHLKKLTRHYKPCNATLKNRGHDMMLKFGEEGSGSITVNGTEYKLLQLHWH 132

Query: 124 SPSEHTIDGKKFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSMIEDHLELASDTHEEE 183
           SPSEHT++G++F LE H+VHE+ NG ++V+ +LY+IG PD FL ++E+ L   +D +E E
Sbjct: 133 SPSEHTMNGRRFALELHMVHENINGSLAVVTVLYKIGRPDSFLGLLENKLSAITDQNEAE 192

Query: 184 RVIGIIDPNLLKMGSKQYYRYIGSLTVPPCTENVMWTMIHQVRT 227
           + + +IDP  +K+GS+++YRYIGSLT PPCT+NV+WT++ +V T
Sbjct: 193 KYVDVIDPRDIKIGSRKFYRYIGSLTTPPCTQNVIWTVVKKVNT 236

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BLAST of Lsi03G000300 vs. TrEMBL
Match: B9STU9_RICCO (Carbonic anhydrase, putative OS=Ricinus communis GN=RCOM_0544250 PE=4 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 8.6e-96
Identity = 163/265 (61.51%), Postives = 212/265 (80.00%), Query Frame = 1


Query: 4   LLCSFFFSILLLSFKAISQEVDDERGFDYERDGEKGPIHWGELRPEWHTCKAGLMQSPID 63
           L  +FFF+ LL +    SQEV++ER FDYE++GE+GP  WGEL  EW  C  G MQSPID
Sbjct: 9   LFLTFFFAFLLHTLPTTSQEVENEREFDYEKNGERGPARWGELHEEWRACSNGSMQSPID 68

Query: 64  LLHERVEVISHLGDLKINYKSSNATLKNRGHDMMMKWVDGGGFMEMNGTQYKLKQCHWHS 123
           +L+ERVEV+SHLG L  +Y+ SNATLKNRGHDMM++W +G G +E+NGT+Y L+QCHWHS
Sbjct: 69  MLNERVEVVSHLGRLNRSYEPSNATLKNRGHDMMLEWENGAGTIEINGTEYILRQCHWHS 128

Query: 124 PSEHTIDGKKFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSMIEDHLELASDTHEEER 183
           PSEHT+DG++F LE H+VHES +G ++V+G++Y IG PD FL  + DHL   S T EEER
Sbjct: 129 PSEHTLDGRRFALELHMVHESPDGKVAVVGVMYTIGRPDSFLLSLTDHLRSISGTEEEER 188

Query: 184 VIGIIDPNLLKMGSKQYYRYIGSLTVPPCTENVMWTMIHQVRTVTEEQVKLLRVAVHDDS 243
           V+GII+P  +K+GS++YYRY+GSLTVPPCTENV+WT++ +VRTVT EQV LLRVAVHD+S
Sbjct: 189 VVGIINPKDIKIGSRKYYRYMGSLTVPPCTENVIWTIVKKVRTVTREQVSLLRVAVHDES 248

Query: 244 DSNARPLQLIYNRTMQLQRSKESEE 269
           D+NARPLQ I  R++ L R +E+ +
Sbjct: 249 DTNARPLQQINGRSVHLYRPEENND 273

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BLAST of Lsi03G000300 vs. TrEMBL
Match: A0A067LIF8_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_05491 PE=4 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 4.3e-95
Identity = 168/267 (62.92%), Postives = 214/267 (80.15%), Query Frame = 1


Query: 3   KLLC-SFFFSILLLSFKAISQEVDDERGFDYERDGEKGPIHWGELRPEWHTCKAGLMQSP 62
           +LLC +FF S+L  +  + SQEV+DER F YE++ E GP  WGEL PEW TC  G MQSP
Sbjct: 7   QLLCFTFFISLLFHALPSSSQEVEDEREFSYEKNSETGPERWGELHPEWSTCSNGTMQSP 66

Query: 63  IDLLHERVEVISHLGDLKINYKSSNATLKNRGHDMMMKWVDGGGFMEMNGTQYKLKQCHW 122
           IDLL+ERVEV+SHLG LK +YK SNATLKNRGHDMM++W +G G +E+NGT+Y L+QCHW
Sbjct: 67  IDLLNERVEVVSHLGKLKRSYKPSNATLKNRGHDMMLEWENGAGTIEINGTEYVLRQCHW 126

Query: 123 HSPSEHTIDGKKFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSMIEDHLELASDTHEE 182
           HSPSEHTIDG +F LE H+VHES +G ++V+GI+Y IG PD FLS + + L   S T EE
Sbjct: 127 HSPSEHTIDGGRFALELHMVHESQDGKVAVVGIMYIIGRPDSFLSSLTEKLREVSGTEEE 186

Query: 183 ERVIGIIDPNLLKMGSKQYYRYIGSLTVPPCTENVMWTMIHQVRTVTEEQVKLLRVAVHD 242
           E V+GI++P  +K+GS++YYRYIGSLTVPPCTENV+WT+  +VRTVT+EQV+LLRVAVHD
Sbjct: 187 ETVVGIVNPKDIKIGSRKYYRYIGSLTVPPCTENVVWTISRKVRTVTKEQVELLRVAVHD 246

Query: 243 DSDSNARPLQLIYNRTMQLQRSKESEE 269
           ++D+NARPLQ    R++QL R +E +E
Sbjct: 247 EADTNARPLQPTNGRSVQLYRPEEYKE 273

[Close]

BLAST of Lsi03G000300 vs. TrEMBL
Match: A0A0A0LV23_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G120450 PE=4 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 7.3e-95
Identity = 163/258 (63.18%), Postives = 209/258 (81.01%), Query Frame = 1


Query: 4   LLCSFFFSILLLSFKAISQEVDDERGFDYERDGEKGPIHWGELRPEWHTCKAGLMQSPID 63
           L CSFFF+ LL+ + A+SQEV+++R FDY  +G +GP HWG LRPEWHTC  G MQSPID
Sbjct: 10  LFCSFFFAFLLVPWPAMSQEVENQREFDYNANGTRGPAHWGVLRPEWHTCNTGQMQSPID 69

Query: 64  LLHERVEVISHLGDLKINYKSSNATLKNRGHDMMMKWVDGGGFMEMNGTQYKLKQCHWHS 123
           LL++RV ++SH  DLKINY SSNATLKNRGHD+M++W    G+ME+N TQY L+Q HWHS
Sbjct: 70  LLNQRVRIVSHFTDLKINYSSSNATLKNRGHDIMLQWSGRAGYMEVNKTQYFLRQIHWHS 129

Query: 124 PSEHTIDGKKFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSMIEDHLELASDTHEEER 183
           PSEHTI+G++F LEAHLVH+S  G I+VIGILY IG+PDYFLS +  HL   S T + +R
Sbjct: 130 PSEHTINGRRFALEAHLVHQSQTGKIAVIGILYNIGQPDYFLSTMRPHLIEISGT-QRDR 189

Query: 184 VIGIIDPNLLKMGSKQYYRYIGSLTVPPCTENVMWTMIHQVRTVTEEQVKLLRVAVHDDS 243
           ++ +++P+LL+M S  YYRYIGSLTVPPC++NV+WT++ +VRTV  EQV LLRVAVHDDS
Sbjct: 190 LLNVVNPSLLRMRSSLYYRYIGSLTVPPCSQNVLWTLVRKVRTVAPEQVNLLRVAVHDDS 249

Query: 244 DSNARPLQLIYNRTMQLQ 262
           ++NARPLQ + NR +QL+
Sbjct: 250 NTNARPLQPLNNRNVQLR 266

[Close]

BLAST of Lsi03G000300 vs. TrEMBL
Match: A0A067GDC1_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g039445mg PE=4 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 9.5e-95
Identity = 166/265 (62.64%), Postives = 209/265 (78.87%), Query Frame = 1


Query: 4   LLCSFFFSILLLSFKAISQEVDDERGFDYERDGEKGPIHWGELRPEWHTCKAGLMQSPID 63
           L   FF  +LLLS  A+SQEVDDE+ F+YE +G++GP  WGEL  EW  CK G MQSPID
Sbjct: 9   LFSCFFLVLLLLSCPAVSQEVDDEKEFNYEEEGQRGPSKWGELHKEWSACKKGTMQSPID 68

Query: 64  LLHERVEVISHLGDLKINYKSSNATLKNRGHDMMMKWVDGGGFMEMNGTQYKLKQCHWHS 123
           LL+ERV+V+SHLG LK +YK S ATLKNRGHDMM++W  G G + +NGT+Y LKQCHWHS
Sbjct: 69  LLNERVQVVSHLGRLKRSYKPSYATLKNRGHDMMLQWHGGAGTLLINGTEYVLKQCHWHS 128

Query: 124 PSEHTIDGKKFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSMIEDHLELASDTHEEER 183
           PSEHTIDG++F LEAH+VHES +G I+V+GI+Y+IG PD  L+ I DHL   + ++E E 
Sbjct: 129 PSEHTIDGERFALEAHMVHESQDGKIAVVGIMYKIGRPDSLLASISDHLRQIAGSNERET 188

Query: 184 VIGIIDPNLLKMGSKQYYRYIGSLTVPPCTENVMWTMIHQVRTVTEEQVKLLRVAVHDDS 243
           V+G+IDP  +K+GS++YYRYIGSLT PPCTENV+WT++ +VRTVT EQVKLLRVAVHDDS
Sbjct: 189 VVGVIDPRGIKLGSRKYYRYIGSLTTPPCTENVIWTIVRKVRTVTREQVKLLRVAVHDDS 248

Query: 244 DSNARPLQLIYNRTMQLQRSKESEE 269
            +NARP Q I  RT++L R  +  +
Sbjct: 249 GTNARPQQPINLRTIKLYRPDDHNQ 273

[Close]

BLAST of Lsi03G000300 vs. TrEMBL
Match: A0A151S5F1_CAJCA (Uncharacterized protein OS=Cajanus cajan GN=KK1_028191 PE=4 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 9.5e-95
Identity = 157/261 (60.15%), Postives = 211/261 (80.84%), Query Frame = 1


Query: 4   LLCSFFFSILLLSFKAISQEVDDERGFDYERDGEKGPIHWGELRPEWHTCKAGLMQSPID 63
           ++C  F ++L LS   +SQEV+DE  F+YE + EKGP HWG++ PEW  CK G MQSPID
Sbjct: 4   IICCLFTALLFLSLPVMSQEVEDESEFNYEENSEKGPSHWGDIHPEWSLCKNGSMQSPID 63

Query: 64  LLHERVEVISHLGDLKINYKSSNATLKNRGHDMMMKWVDGGGFMEMNGTQYKLKQCHWHS 123
           LL+ERVE++SHLG L+INY+ SN T+KNRGHD+ ++WV G G++++N T+Y LKQCHWHS
Sbjct: 64  LLNERVEILSHLGRLQINYQPSNGTIKNRGHDIKLEWVSGAGYLQINETKYVLKQCHWHS 123

Query: 124 PSEHTIDGKKFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSMIEDHLELASDTHEEER 183
           PSEHTIDGK+FDLE H+VHE+ +G  +VIGIL++IG PD FLS + +HL+  SD+ E ER
Sbjct: 124 PSEHTIDGKRFDLELHMVHETPSGQTAVIGILFKIGRPDSFLSSLTNHLKAISDSTEAER 183

Query: 184 VIGIIDPNLLKMGSKQYYRYIGSLTVPPCTENVMWTMIHQVRTVTEEQVKLLRVAVHDDS 243
           V+G++DP  +K+  KQYYRY GSLT+PPCT+NV WT++ ++R+V++EQV+LLRVAVHD+S
Sbjct: 184 VVGVVDPRQIKVVYKQYYRYNGSLTIPPCTQNVSWTILREIRSVSKEQVRLLRVAVHDES 243

Query: 244 DSNARPLQLIYNRTMQLQRSK 265
           ++NARPLQ I NR +QL   K
Sbjct: 244 NTNARPLQPINNRLLQLNGPK 264

[Close]

BLAST of Lsi03G000300 vs. TAIR10
Match: AT1G08080.1 (AT1G08080.1 alpha carbonic anhydrase 7)

HSP 1 Score: 305.1 bits (780), Expect = 4.4e-83
Identity = 144/264 (54.55%), Postives = 197/264 (74.62%), Query Frame = 1


Query: 6   CSFF------FSILLLSFKAISQ-EVDDERGFDYERDGEKGPIHWGELRPEWHTCKAGLM 65
           C FF      F+I+ +S  A S  EV+DER F+Y+++ EKGP  WGEL+PEW  C  G M
Sbjct: 9   CIFFVALFSIFTIVSISSAASSHGEVEDEREFNYKKNDEKGPERWGELKPEWEMCGKGEM 68

Query: 66  QSPIDLLHERVEVISHLGDLKINYKSSNATLKNRGHDMMMKWVDGGGFMEMNGTQYKLKQ 125
           QSPIDL++ERV ++SHLG L  +Y  SNATLKNRGHD+M+K+ DG G +++NG +Y+L+Q
Sbjct: 69  QSPIDLMNERVNIVSHLGRLNRDYNPSNATLKNRGHDIMLKFEDGAGTIKINGFEYELQQ 128

Query: 126 CHWHSPSEHTIDGKKFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSMIEDHLELASDT 185
            HWHSPSEHTI+G++F LE H+VHE  N  ++V+ +LY+IG  D F+  +E  LE  ++ 
Sbjct: 129 LHWHSPSEHTINGRRFALELHMVHEGRNRRMAVVTVLYKIGRADTFIRSLEKELEGIAEM 188

Query: 186 HEEERVIGIIDPNLLKMGSKQYYRYIGSLTVPPCTENVMWTMIHQVRTVTEEQVKLLRVA 245
            E E+ +G+IDP  +K+GS++YYRY GSLT PPCT+NV W+++ +VRTVT +QVKLLRVA
Sbjct: 189 EEAEKNVGMIDPTKIKIGSRKYYRYTGSLTTPPCTQNVTWSVVRKVRTVTRKQVKLLRVA 248

Query: 246 VHDDSDSNARPLQLIYNRTMQLQR 263
           VHDD++SNARP+Q    R + L R
Sbjct: 249 VHDDANSNARPVQPTNKRIVHLYR 272

[Close]

BLAST of Lsi03G000300 vs. TAIR10
Match: AT1G08065.1 (AT1G08065.1 alpha carbonic anhydrase 5)

HSP 1 Score: 276.2 bits (705), Expect = 2.2e-74
Identity = 133/262 (50.76%), Postives = 183/262 (69.85%), Query Frame = 1


Query: 1   MGKLLCSFFFSILLLSFKAISQEVDDERGFDYERDGEKGPIHWGELRPEWHTCKAGLMQS 60
           +G +    F SI ++S      EV+DE  F+YE+ GEKGP +WG L+PEW  C  G MQS
Sbjct: 6   IGYVFFLIFISITIVSSSPDHGEVEDETQFNYEKKGEKGPENWGRLKPEWAMCGKGNMQS 65

Query: 61  PIDLLHERVEVISHLGDLKINYKSSNATLKNRGHDMMMKWVDG--GGFMEMNGTQYKLKQ 120
           PIDL  +RV +  +LG L+  Y  SNAT+KNRGHD+MMK+  G  G  + +NGT+YKL+Q
Sbjct: 66  PIDLTDKRVLIDHNLGYLRSQYLPSNATIKNRGHDIMMKFEGGNAGLGITINGTEYKLQQ 125

Query: 121 CHWHSPSEHTIDGKKFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSMIEDHLELASDT 180
            HWHSPSEHT++GK+F LE H+VH+S +G  +V+   Y++G+PDYFL  +E +L+  +DT
Sbjct: 126 IHWHSPSEHTLNGKRFVLEEHMVHQSKDGRNAVVAFFYKLGKPDYFLLTLERYLKRITDT 185

Query: 181 HEEERVIGIIDPNLLKMGSKQYYRYIGSLTVPPCTENVMWTMIHQVRTVTEEQVKLLRVA 240
           HE +  + ++ P      SK YYR+IGSLT PPC+ENV+WT+  ++RTVT +Q+ +LRV 
Sbjct: 186 HESQEFVEMVHPRTFGFESKHYYRFIGSLTTPPCSENVIWTISKEMRTVTLKQLIMLRVT 245

Query: 241 VHDDSDSNARPLQLIYNRTMQL 261
           VHD S+SNARPLQ    R + L
Sbjct: 246 VHDQSNSNARPLQRKNERPVAL 267

[Close]

BLAST of Lsi03G000300 vs. TAIR10
Match: AT2G28210.1 (AT2G28210.1 alpha carbonic anhydrase 2)

HSP 1 Score: 253.4 bits (646), Expect = 1.5e-67
Identity = 111/208 (53.37%), Postives = 158/208 (75.96%), Query Frame = 1


Query: 20  ISQEVDDERGFDYERDGEKGPIHWGELRPEWHTCKAGLMQSPIDLLHERVEVISHLGDLK 79
           + + V+DE  F YE + E GP  WG+LRPEW  C  G MQSPIDL+++RV +++HL  L 
Sbjct: 1   MDEYVEDEHEFSYEWNQENGPAKWGKLRPEWKMCGKGEMQSPIDLMNKRVRLVTHLKKLT 60

Query: 80  INYKSSNATLKNRGHDMMMKW-VDGGGFMEMNGTQYKLKQCHWHSPSEHTIDGKKFDLEA 139
            +YK  NATLKNRGHDMM+K+  +G G + +NGT+YKL Q HWHSPSEHT++G++F LE 
Sbjct: 61  RHYKPCNATLKNRGHDMMLKFGEEGSGSITVNGTEYKLLQLHWHSPSEHTMNGRRFALEL 120

Query: 140 HLVHESSNGMISVIGILYQIGEPDYFLSMIEDHLELASDTHEEERVIGIIDPNLLKMGSK 199
           H+VHE+ NG ++V+ +LY+IG PD FL ++E+ L   +D +E E+ + +IDP  +K+GS+
Sbjct: 121 HMVHENINGSLAVVTVLYKIGRPDSFLGLLENKLSAITDQNEAEKYVDVIDPRDIKIGSR 180

Query: 200 QYYRYIGSLTVPPCTENVMWTMIHQVRT 227
           ++YRYIGSLT PPCT+NV+WT++ +V T
Sbjct: 181 KFYRYIGSLTTPPCTQNVIWTVVKKVNT 208

[Close]

BLAST of Lsi03G000300 vs. TAIR10
Match: AT4G20990.1 (AT4G20990.1 alpha carbonic anhydrase 4)

HSP 1 Score: 235.7 bits (600), Expect = 3.3e-62
Identity = 115/249 (46.18%), Postives = 166/249 (66.67%), Query Frame = 1


Query: 8   FFFSI--LLLSFKAISQ---EVDDERGFDYERDGEKGPIHWGELRPEWHTCKAGLMQSPI 67
           FF ++  + LSF  IS    EVDDE  F YE+  EKGP  WG++ P W  C  G  QSPI
Sbjct: 9   FFMAMCFIYLSFPNISHAHSEVDDETPFTYEQKTEKGPEGWGKINPHWKVCNTGRYQSPI 68

Query: 68  DLLHERVEVISHLGDLKINYKSSNATLKNRGHDMMMKWVDGGGFMEMNGTQYKLKQCHWH 127
           DL +ERV +I H       YK + A + NRGHD+M+ W    G M +  T + L QCHWH
Sbjct: 69  DLTNERVSLI-HDQAWTRQYKPAPAVITNRGHDIMVSWKGDAGKMTIRKTDFNLVQCHWH 128

Query: 128 SPSEHTIDGKKFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSMIEDHLELASDTHEEE 187
           SPSEHT++G ++DLE H+VH S+ G  +VIG+LY++GEP+ FL+ + + ++   +   +E
Sbjct: 129 SPSEHTVNGTRYDLELHMVHTSARGRTAVIGVLYKLGEPNEFLTKLLNGIKAVGN---KE 188

Query: 188 RVIGIIDPNLLKMGSKQYYRYIGSLTVPPCTENVMWTMIHQVRTVTEEQVKLLRVAVHDD 247
             +G+IDP  ++  ++++YRYIGSLTVPPCTE V+WT++ +V T++ EQ+  LR AV D 
Sbjct: 189 INLGMIDPREIRFQTRKFYRYIGSLTVPPCTEGVIWTVVKRVNTISMEQITALRQAVDDG 248

Query: 248 SDSNARPLQ 252
            ++N+RP+Q
Sbjct: 249 FETNSRPVQ 253

[Close]

BLAST of Lsi03G000300 vs. TAIR10
Match: AT4G21000.1 (AT4G21000.1 alpha carbonic anhydrase 6)

HSP 1 Score: 209.9 bits (533), Expect = 1.9e-54
Identity = 96/204 (47.06%), Postives = 141/204 (69.12%), Query Frame = 1


Query: 21  SQEVDDERGFDYERDGEKGPIHWGELRPEWHTCKAGLMQSPIDLLHERVEVISHLGDLKI 80
           ++E+ ++  F Y++  EKGP  WG+L P+W  C  G +QSPIDL  ERV +I H   L  
Sbjct: 28  AREIGNKPLFTYKQKTEKGPAEWGKLDPQWKVCSTGKIQSPIDLTDERVSLI-HDQALSK 87

Query: 81  NYKSSNATLKNRGHDMMMKWVDGGGFMEMNGTQYKLKQCHWHSPSEHTIDGKKFDLEAHL 140
           +YK ++A +++RGHD+M+ W   GG + ++ T YKL QCHWHSPSEHTI+G  +DLE H+
Sbjct: 88  HYKPASAVIQSRGHDVMVSWKGDGGKITIHQTDYKLVQCHWHSPSEHTINGTSYDLELHM 147

Query: 141 VHESSNGMISVIGILYQIGEPDYFLSMIEDHLELASDTHEEERVIGIIDPNLLKMGSKQY 200
           VH S++G  +V+G+LY++GEPD FL+ I   L       ++E  +GI+DP  ++  +  +
Sbjct: 148 VHTSASGKTTVVGVLYKLGEPDEFLTKI---LNGIKGVGKKEIDLGIVDPRDIRFETNNF 207

Query: 201 YRYIGSLTVPPCTENVMWTMIHQV 225
           YRYIGSLT+PPCTE V+WT+  +V
Sbjct: 208 YRYIGSLTIPPCTEGVIWTVQKRV 227

[Close]

BLAST of Lsi03G000300 vs. NCBI nr
Match: gi|449443578|ref|XP_004139554.1| (PREDICTED: alpha carbonic anhydrase 7-like [Cucumis sativus])

HSP 1 Score: 500.0 bits (1286), Expect = 2.6e-138
Identity = 241/269 (89.59%), Postives = 254/269 (94.42%), Query Frame = 1


Query: 1   MGKLLCSF-FFSILLLSFKAISQEVDDERGFDYERDGEKGPIHWGELRPEWHTCKAGLMQ 60
           MG +LCS  FFS+LL S KA SQEV++ERGFDYERDGEKGP HWGELR EWH CK GLMQ
Sbjct: 1   MGNILCSLIFFSLLLFSLKATSQEVENERGFDYERDGEKGPTHWGELRAEWHKCKGGLMQ 60

Query: 61  SPIDLLHERVEVISHLGDLKINYKSSNATLKNRGHDMMMKWVDGGGFMEMNGTQYKLKQC 120
           SPIDLLHERVEVISHLGDL+INYKSSNATLKNRGHDMMMKWVDGGG+MEMNGTQYKLKQC
Sbjct: 61  SPIDLLHERVEVISHLGDLQINYKSSNATLKNRGHDMMMKWVDGGGYMEMNGTQYKLKQC 120

Query: 121 HWHSPSEHTIDGKKFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSMIEDHLELASDTH 180
           HWHSPSEHTIDGK FDLEAHLVHESSNGMISVIGILYQIGEPDYFLS IE+ LE+AS+TH
Sbjct: 121 HWHSPSEHTIDGKTFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSTIEEDLEVASNTH 180

Query: 181 EEERVIGIIDPNLLKMGSKQYYRYIGSLTVPPCTENVMWTMIHQVRTVTEEQVKLLRVAV 240
           EEERVIGIIDPN+LKMGSKQYYRYIGSLTVPPCTENVMWT+IHQVRTVTEEQVKLLRVAV
Sbjct: 181 EEERVIGIIDPNILKMGSKQYYRYIGSLTVPPCTENVMWTIIHQVRTVTEEQVKLLRVAV 240

Query: 241 HDDSDSNARPLQLIYNRTMQLQRSKESEE 269
           HDDSDSNARPLQ  Y+RTMQLQRSKESE+
Sbjct: 241 HDDSDSNARPLQPFYDRTMQLQRSKESEQ 269

[Close]

BLAST of Lsi03G000300 vs. NCBI nr
Match: gi|659072266|ref|XP_008464591.1| (PREDICTED: LOW QUALITY PROTEIN: bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like [Cucumis melo])

HSP 1 Score: 498.0 bits (1281), Expect = 1.0e-137
Identity = 239/268 (89.18%), Postives = 255/268 (95.15%), Query Frame = 1


Query: 1   MGKLLCSFFFSILLL-SFKAISQEVDDERGFDYERDGEKGPIHWGELRPEWHTCKAGLMQ 60
           MGK+LCS  FS LLL S KA SQEV+DERGFDYERDGEKGP+HWGELR EWH CK GLMQ
Sbjct: 1   MGKILCSLIFSSLLLFSLKARSQEVEDERGFDYERDGEKGPVHWGELRAEWHKCKGGLMQ 60

Query: 61  SPIDLLHERVEVISHLGDLKINYKSSNATLKNRGHDMMMKWVDGGGFMEMNGTQYKLKQC 120
           SPIDLLHERVEVISHLGDL+INYKSSNATLKNRGHDMMMKWVDGGG+MEMNGTQYKLKQC
Sbjct: 61  SPIDLLHERVEVISHLGDLQINYKSSNATLKNRGHDMMMKWVDGGGYMEMNGTQYKLKQC 120

Query: 121 HWHSPSEHTIDGKKFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSMIEDHLELASDTH 180
           HWHSPSEHTIDGK FDLEAHLVHESSNGM+SVIGILYQIGEPDYFLS I++ LE+AS+TH
Sbjct: 121 HWHSPSEHTIDGKNFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKEDLEVASNTH 180

Query: 181 EEERVIGIIDPNLLKMGSKQYYRYIGSLTVPPCTENVMWTMIHQVRTVTEEQVKLLRVAV 240
           EEERV+GIIDPN+LKMGSKQYYRYIGSLTVPPCTE+VMWT+IHQVRTVTEEQVKLLRVAV
Sbjct: 181 EEERVVGIIDPNILKMGSKQYYRYIGSLTVPPCTEDVMWTIIHQVRTVTEEQVKLLRVAV 240

Query: 241 HDDSDSNARPLQLIYNRTMQLQRSKESE 268
           HDDS+SNARPLQ IYNRTMQLQRSK+SE
Sbjct: 241 HDDSNSNARPLQPIYNRTMQLQRSKDSE 268

[Close]

BLAST of Lsi03G000300 vs. NCBI nr
Match: gi|255577068|ref|XP_002529418.1| (PREDICTED: alpha carbonic anhydrase 7 [Ricinus communis])

HSP 1 Score: 358.2 bits (918), Expect = 1.2e-95
Identity = 163/265 (61.51%), Postives = 212/265 (80.00%), Query Frame = 1


Query: 4   LLCSFFFSILLLSFKAISQEVDDERGFDYERDGEKGPIHWGELRPEWHTCKAGLMQSPID 63
           L  +FFF+ LL +    SQEV++ER FDYE++GE+GP  WGEL  EW  C  G MQSPID
Sbjct: 9   LFLTFFFAFLLHTLPTTSQEVENEREFDYEKNGERGPARWGELHEEWRACSNGSMQSPID 68

Query: 64  LLHERVEVISHLGDLKINYKSSNATLKNRGHDMMMKWVDGGGFMEMNGTQYKLKQCHWHS 123
           +L+ERVEV+SHLG L  +Y+ SNATLKNRGHDMM++W +G G +E+NGT+Y L+QCHWHS
Sbjct: 69  MLNERVEVVSHLGRLNRSYEPSNATLKNRGHDMMLEWENGAGTIEINGTEYILRQCHWHS 128

Query: 124 PSEHTIDGKKFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSMIEDHLELASDTHEEER 183
           PSEHT+DG++F LE H+VHES +G ++V+G++Y IG PD FL  + DHL   S T EEER
Sbjct: 129 PSEHTLDGRRFALELHMVHESPDGKVAVVGVMYTIGRPDSFLLSLTDHLRSISGTEEEER 188

Query: 184 VIGIIDPNLLKMGSKQYYRYIGSLTVPPCTENVMWTMIHQVRTVTEEQVKLLRVAVHDDS 243
           V+GII+P  +K+GS++YYRY+GSLTVPPCTENV+WT++ +VRTVT EQV LLRVAVHD+S
Sbjct: 189 VVGIINPKDIKIGSRKYYRYMGSLTVPPCTENVIWTIVKKVRTVTREQVSLLRVAVHDES 248

Query: 244 DSNARPLQLIYNRTMQLQRSKESEE 269
           D+NARPLQ I  R++ L R +E+ +
Sbjct: 249 DTNARPLQQINGRSVHLYRPEENND 273

[Close]

BLAST of Lsi03G000300 vs. NCBI nr
Match: gi|802554827|ref|XP_012065108.1| (PREDICTED: alpha carbonic anhydrase 7-like [Jatropha curcas])

HSP 1 Score: 355.9 bits (912), Expect = 6.1e-95
Identity = 168/267 (62.92%), Postives = 214/267 (80.15%), Query Frame = 1


Query: 3   KLLC-SFFFSILLLSFKAISQEVDDERGFDYERDGEKGPIHWGELRPEWHTCKAGLMQSP 62
           +LLC +FF S+L  +  + SQEV+DER F YE++ E GP  WGEL PEW TC  G MQSP
Sbjct: 7   QLLCFTFFISLLFHALPSSSQEVEDEREFSYEKNSETGPERWGELHPEWSTCSNGTMQSP 66

Query: 63  IDLLHERVEVISHLGDLKINYKSSNATLKNRGHDMMMKWVDGGGFMEMNGTQYKLKQCHW 122
           IDLL+ERVEV+SHLG LK +YK SNATLKNRGHDMM++W +G G +E+NGT+Y L+QCHW
Sbjct: 67  IDLLNERVEVVSHLGKLKRSYKPSNATLKNRGHDMMLEWENGAGTIEINGTEYVLRQCHW 126

Query: 123 HSPSEHTIDGKKFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSMIEDHLELASDTHEE 182
           HSPSEHTIDG +F LE H+VHES +G ++V+GI+Y IG PD FLS + + L   S T EE
Sbjct: 127 HSPSEHTIDGGRFALELHMVHESQDGKVAVVGIMYIIGRPDSFLSSLTEKLREVSGTEEE 186

Query: 183 ERVIGIIDPNLLKMGSKQYYRYIGSLTVPPCTENVMWTMIHQVRTVTEEQVKLLRVAVHD 242
           E V+GI++P  +K+GS++YYRYIGSLTVPPCTENV+WT+  +VRTVT+EQV+LLRVAVHD
Sbjct: 187 ETVVGIVNPKDIKIGSRKYYRYIGSLTVPPCTENVVWTISRKVRTVTKEQVELLRVAVHD 246

Query: 243 DSDSNARPLQLIYNRTMQLQRSKESEE 269
           ++D+NARPLQ    R++QL R +E +E
Sbjct: 247 EADTNARPLQPTNGRSVQLYRPEEYKE 273

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BLAST of Lsi03G000300 vs. NCBI nr
Match: gi|1009115852|ref|XP_015874453.1| (PREDICTED: alpha carbonic anhydrase 7 [Ziziphus jujuba])

HSP 1 Score: 355.5 bits (911), Expect = 8.0e-95
Identity = 166/262 (63.36%), Postives = 207/262 (79.01%), Query Frame = 1


Query: 4   LLCSFFFSILLLSFKAISQEVDDERGFDYERDGEKGPIHWGELRPEWHTCKAGLMQSPID 63
           LLCSFF  ++L    A SQEV+DE  F Y    E GP  WGE+RPEW  C  G MQSPID
Sbjct: 9   LLCSFFIGLVLNLSPATSQEVEDESEFSYVEGSENGPSRWGEIRPEWSICSNGSMQSPID 68

Query: 64  LLHERVEVISHLGDLKINYKSSNATLKNRGHDMMMKWVDGGGFMEMNGTQYKLKQCHWHS 123
           LL ERVEV+SHLG LK+NYK+SNATLKNRGHDMM+KW    G +E+NGT+Y L+QCHWH+
Sbjct: 69  LLSERVEVVSHLGRLKMNYKASNATLKNRGHDMMLKWEGDAGNLEINGTEYALRQCHWHT 128

Query: 124 PSEHTIDGKKFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSMIEDHLELASDTHEEER 183
           PSEHT++GKK+D+E HLVHES  G I+VIGILY+IG PD FLS +ED LE  S + E ER
Sbjct: 129 PSEHTVNGKKYDVEVHLVHESPQGNITVIGILYKIGRPDSFLSSMEDILEEISGSEEGER 188

Query: 184 VIGIIDPNLLKMGSKQYYRYIGSLTVPPCTENVMWTMIHQVRTVTEEQVKLLRVAVHDDS 243
           V+GI++P  +K+GS++YYRYIGSLT PPCT+NV+WT++ +VRTVT +Q++LLRVAVHDDS
Sbjct: 189 VVGIVNPKNIKIGSRKYYRYIGSLTTPPCTQNVVWTIVKKVRTVTRKQMRLLRVAVHDDS 248

Query: 244 DSNARPLQLIYNRTMQLQRSKE 266
            +NARPLQ I  R++ + R +E
Sbjct: 249 HTNARPLQAINKRSVLMYRPRE 270

The following BLAST results are available for this feature:

BLAST of Lsi03G000300 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ATCA7_ARATH	7.8e-82	54.55	Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana GN=ACA7 PE=2 SV=1	[more]
NEC3_NICLS	1.6e-74	55.51	Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3 OS...	[more]
ATCA5_ARATH	3.9e-73	50.76	Alpha carbonic anhydrase 5 OS=Arabidopsis thaliana GN=ACA5 PE=3 SV=1	[more]
ATCA8_ARATH	8.9e-70	53.69	Alpha carbonic anhydrase 8 OS=Arabidopsis thaliana GN=ACA8 PE=3 SV=3	[more]
ATCA2_ARATH	8.3e-68	52.23	Alpha carbonic anhydrase 2 OS=Arabidopsis thaliana GN=ACA2 PE=2 SV=2	[more]

BLAST of Lsi03G000300 vs. TrEMBL


Match Name	E-value	Identity	Description
B9STU9_RICCO	8.6e-96	61.51	Carbonic anhydrase, putative OS=Ricinus communis GN=RCOM_0544250 PE=4 SV=1	[more]
A0A067LIF8_JATCU	4.3e-95	62.92	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_05491 PE=4 SV=1	[more]
A0A0A0LV23_CUCSA	7.3e-95	63.18	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G120450 PE=4 SV=1	[more]
A0A067GDC1_CITSI	9.5e-95	62.64	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g039445mg PE=4 SV=1	[more]
A0A151S5F1_CAJCA	9.5e-95	60.15	Uncharacterized protein OS=Cajanus cajan GN=KK1_028191 PE=4 SV=1	[more]

BLAST of Lsi03G000300 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G08080.1	4.4e-83	54.55	alpha carbonic anhydrase 7	[more]
AT1G08065.1	2.2e-74	50.76	alpha carbonic anhydrase 5	[more]
AT2G28210.1	1.5e-67	53.37	alpha carbonic anhydrase 2	[more]
AT4G20990.1	3.3e-62	46.18	alpha carbonic anhydrase 4	[more]
AT4G21000.1	1.9e-54	47.06	alpha carbonic anhydrase 6	[more]

BLAST of Lsi03G000300 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449443578\|ref\|XP_004139554.1\|	2.6e-138	89.59	PREDICTED: alpha carbonic anhydrase 7-like [Cucumis sativus]	[more]
gi\|659072266\|ref\|XP_008464591.1\|	1.0e-137	89.18	PREDICTED: LOW QUALITY PROTEIN: bifunctional monodehydroascorbate reductase and ...	[more]
gi\|255577068\|ref\|XP_002529418.1\|	1.2e-95	61.51	PREDICTED: alpha carbonic anhydrase 7 [Ricinus communis]	[more]
gi\|802554827\|ref\|XP_012065108.1\|	6.1e-95	62.92	PREDICTED: alpha carbonic anhydrase 7-like [Jatropha curcas]	[more]
gi\|1009115852\|ref\|XP_015874453.1\|	8.0e-95	63.36	PREDICTED: alpha carbonic anhydrase 7 [Ziziphus jujuba]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0008270	zinc ion binding
GO:0004089	carbonate dehydratase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006730	one-carbon metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR023561	Carbonic_anhydrase_a-class
IPR018338	Carbonic_anhydrase_a-class_CS
IPR001148	CA_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006807	nitrogen compound metabolic process
biological_process	GO:0006730	one-carbon metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004089	carbonate dehydratase activity
molecular_function	GO:0008270	zinc ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G000300.1	Lsi03G000300.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001148	Alpha carbonic anhydrase	GENE3D	G3DSA:3.10.200.10		coord: 28..258 score: 3.4
IPR001148	Alpha carbonic anhydrase	PFAM	PF00194	Carb_anhydrase	coord: 32..259 score: 3.3
IPR001148	Alpha carbonic anhydrase	SMART	SM01057	Carb_anhydrase_2a	coord: 32..262 score: 7.6
IPR001148	Alpha carbonic anhydrase	PROFILE	PS51144	ALPHA_CA_2	coord: 28..262 score: 43
IPR001148	Alpha carbonic anhydrase	unknown	SSF51069	Carbonic anhydrase	coord: 29..258 score: 8.64
IPR018338	Carbonic anhydrase, alpha-class, conserved site	PROSITE	PS00162	ALPHA_CA_1	coord: 125..141 scor
IPR023561	Carbonic anhydrase, alpha-class	PANTHER	PTHR18952	CARBONIC ANHYDRASE	coord: 4..268 score: 7.2E
None	No IPR available	PANTHER	PTHR18952:SF111	ALPHA CARBONIC ANHYDRASE 3	coord: 4..268 score: 7.2E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi03G000300	CsGy1G013710	Cucumber (Gy14) v2	cgyblsiB041

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi03G000300	Wax gourd	lsiwgoB337
Lsi03G000300	Bottle gourd (USVL1VR-Ls)	lsilsiB041
Lsi03G000300	Cucumber (Gy14) v1	cgylsiB353
Lsi03G000300	Cucurbita maxima (Rimu)	cmalsiB212
Lsi03G000300	Cucurbita maxima (Rimu)	cmalsiB423
Lsi03G000300	Cucurbita maxima (Rimu)	cmalsiB525
Lsi03G000300	Cucurbita moschata (Rifu)	cmolsiB203
Lsi03G000300	Cucurbita moschata (Rifu)	cmolsiB410
Lsi03G000300	Cucurbita moschata (Rifu)	cmolsiB510
Lsi03G000300	Cucurbita pepo (Zucchini)	cpelsiB242
Lsi03G000300	Cucurbita pepo (Zucchini)	cpelsiB429
Lsi03G000300	Cucurbita pepo (Zucchini)	cpelsiB453
Lsi03G000300	Wild cucumber (PI 183967)	cpilsiB046
Lsi03G000300	Wild cucumber (PI 183967)	cpilsiB456
Lsi03G000300	Cucumber (Chinese Long) v2	culsiB044
Lsi03G000300	Cucumber (Chinese Long) v2	culsiB449
Lsi03G000300	Melon (DHL92) v3.5.1	lsimeB243
Lsi03G000300	Melon (DHL92) v3.5.1	lsimeB244
Lsi03G000300	Watermelon (Charleston Gray)	lsiwcgB237
Lsi03G000300	Watermelon (Charleston Gray)	lsiwcgB238
Lsi03G000300	Watermelon (97103) v1	lsiwmB249
Lsi03G000300	Watermelon (97103) v1	lsiwmB274
Lsi03G000300	Cucumber (Gy14) v2	cgyblsiB415
Lsi03G000300	Melon (DHL92) v3.6.1	lsimedB254
Lsi03G000300	Melon (DHL92) v3.6.1	lsimedB255
Lsi03G000300	Silver-seed gourd	carlsiB149
Lsi03G000300	Silver-seed gourd	carlsiB310
Lsi03G000300	Silver-seed gourd	carlsiB404
Lsi03G000300	Cucumber (Chinese Long) v3	cuclsiB048
Lsi03G000300	Cucumber (Chinese Long) v3	cuclsiB478
Lsi03G000300	Watermelon (97103) v2	lsiwmbB231
Lsi03G000300	Watermelon (97103) v2	lsiwmbB232