Lsi03G000150 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi03G000150
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Xyloglucan endotransglucosylase/hydrolase
Location	chr03 : 243285 .. 245106 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCATTATTTTCTTATCCTTCTTTCTCTCTTTTTGTTCTTCTCTTTCACCTCTTGCCAGAATCCTCCCTCCCCTGGCTATTTTCCTAGCAACCAAGTTCATTCCATTAGCTTCGATCAAGGTTTTCGAAATCGATGGGGCTCTCAACACCAAAAAGTCGACCAAAGAACCTTAACTATCTGGCTCGACAGTTCATCAGGTATATTGTAACCTATCAACTTAAACTTTTAAGTTCACTCGTACTTCTTTTAAGTTCACTCATACTTTAACATGGTATATTGATCAATACAAAGTTTTGTGTCATTTGTTTTTCCTAATTAGTGTTGATTGTTAGATAATATATAAGAATGATAAAATTTATAGTCAGTAACTTAATTGATTGTGGAATTTTAACTATAACTTTTTTTATTCTTCTTAAACAGGGAGTGGATTCAAGTCCCTCCATAGATATCAATCAGGCTACTTTGGAGCTGCCATTAAGCTTCATCCTGGCTACACTGCAGGAGTTATCACTTCTTTTTATGTAAGGATTGTTGTATATCACATCATTCATTAATTTTACTCGTTTAACGTAATGGGTAATTTAACATAGTGTTAGAGGATGAAATCTTTTTTGAATTTTATATTGTTTTTGAGGTTACTTGTTGGATATGAATATATAATTAAATTTACAATAATCTAATGTTAGTTTGACGTTTTGAGAGTTTAGTGGTGATTTAATTTACTTACTAATGTTTATCATTTTTCTTTTTGGATTTTGTAGCTTTCGAACAACGAAGATTATCCGGGAAACCACGACGAGATTGACATTGAGTTTCTCGGAACAACATTGGACAAGCCATATGTTCTTCAAACAAATGTGTTTATGAGAGGGAGTGGAGATGGAAATATTATAGGGAGGGAAATGAGGTTCCATCTTTGGTTTGACCCAACTCAAGATTTTCACAATTACGCCATTTTATGGACTCCAGAAGAAATCATGTATGTTTCAAAACTAACATACTATAAAAAAAAAGTTACATGTTTGGTCCCTGTAATTTCAAACCATCTATAAATTTTTATCCATACAACATGCAGATTCTTAGTGGACGACGTGCCAATCAGGAGGTACGAAAGGAAGAGCGACGCAACATTTCCAGTGAGGCCAATGTGGGTTTACGGGTCAATATGGGATGCATCATCATGGGCAACAGAGGACGGAAAATACAAAGCTGATTACAAATACCAACCGTTTATCGGAAGATACAACAACTTCAAGCTAGGTGGGTGTGCGGCAGGCGGGGCAGCATCATGTCGTCCGCTCAACAGCGGACCAGGCGGAGGTGGTCGCAGCGGGATGAGCCAGCAGCAGGAAAAGTCCATGGAGTGGGTGCAAAGCAATTATTTGGTGTACAACTACTGTCATGATCCAAGAAGAGATCACACTCTCACTCCAGAGTGTTGATTAAAAAAAAAAAAAGACTCACTTTTTTTTTCCCTTTTGGCATTAAAAAACCTGAATATAATACTTGTGTGATTGTTATTAAGATTGAATTGTTCAATGATTAAGAAAATTATGATTTTTGGTTTTTATAAAGAAAAAAAAGTTGGGAGAGATTTGAGAGAGGTCAAGAAAAAGAAAGTTGTGTTAGGCCTTCCAAGTCTTTCCAAGGATTGTATTTCCATTGGCCCATTTCCCTTTTTCTTTTGTTTTGTTTTCTTACTATTAATAGAATAAATGAAATCATCTCTTGGGTCCCACGGATTCTCTGAAGATGCCATTTCTATGAAACATCTTTTGTCGCTGTCTCTTCCCATATAAAATAAACGGTTAAACTAC

mRNA sequence

ATGGCTCATTATTTTCTTATCCTTCTTTCTCTCTTTTTGTTCTTCTCTTTCACCTCTTGCCAGAATCCTCCCTCCCCTGGCTATTTTCCTAGCAACCAAGTTCATTCCATTAGCTTCGATCAAGGTTTTCGAAATCGATGGGGCTCTCAACACCAAAAAGTCGACCAAAGAACCTTAACTATCTGGCTCGACAGTTCATCAGGTATATTTGTTGATTGGAGTGGATTCAAGTCCCTCCATAGATATCAATCAGGCTACTTTGGAGCTGCCATTAAGCTTCATCCTGGCTACACTGCAGGAGTTATCACTTCTTTTTATCTTTCGAACAACGAAGATTATCCGGGAAACCACGACGAGATTGACATTGAGTTTCTCGGAACAACATTGGACAAGCCATATGTTCTTCAAACAAATGTGTTTATGAGAGGGAGTGGAGATGGAAATATTATAGGGAGGGAAATGAGGTTCCATCTTTGGTTTGACCCAACTCAAGATTTTCACAATTACGCCATTTTATGGACTCCAGAAGAAATCATATTCTTAGTGGACGACGTGCCAATCAGGAGGTACGAAAGGAAGAGCGACGCAACATTTCCAGTGAGGCCAATGTGGGTTTACGGGTCAATATGGGATGCATCATCATGGGCAACAGAGGACGGAAAATACAAAGCTGATTACAAATACCAACCGTTTATCGGAAGATACAACAACTTCAAGCTAGGTGGGTGTGCGGCAGGCGGGGCAGCATCATGTCGTCCGCTCAACAGCGGACCAGGCGGAGGTGGTCGCAGCGGGATGAGCCAGCAGCAGGAAAAGTCCATGGAGTGGGTGCAAAGCAATTATTTGGTGTACAACTACTGTCATGATCCAAGAAGAGATCACACTCTCACTCCAGAGTGTTGATTAAAAAAAAAAAAAGACTCACTTTTTTTTTCCCTTTTGGCATTAAAAAACCTGAATATAATACTTGTGTGATTGTTATTAAGATTGAATTGTTCAATGATTAAGAAAATTATGATTTTTGGTTTTTATAAAGAAAAAAAAGTTGGGAGAGATTTGAGAGAGGTCAAGAAAAAGAAAGTTGTGTTAGGCCTTCCAAGTCTTTCCAAGGATTGTATTTCCATTGGCCCATTTCCCTTTTTCTTTTGTTTTGTTTTCTTACTATTAATAGAATAAATGAAATCATCTCTTGGGTCCCACGGATTCTCTGAAGATGCCATTTCTATGAAACATCTTTTGTCGCTGTCTCTTCCCATATAAAATAAACGGTTAAACTAC

Coding sequence (CDS)

ATGGCTCATTATTTTCTTATCCTTCTTTCTCTCTTTTTGTTCTTCTCTTTCACCTCTTGCCAGAATCCTCCCTCCCCTGGCTATTTTCCTAGCAACCAAGTTCATTCCATTAGCTTCGATCAAGGTTTTCGAAATCGATGGGGCTCTCAACACCAAAAAGTCGACCAAAGAACCTTAACTATCTGGCTCGACAGTTCATCAGGTATATTTGTTGATTGGAGTGGATTCAAGTCCCTCCATAGATATCAATCAGGCTACTTTGGAGCTGCCATTAAGCTTCATCCTGGCTACACTGCAGGAGTTATCACTTCTTTTTATCTTTCGAACAACGAAGATTATCCGGGAAACCACGACGAGATTGACATTGAGTTTCTCGGAACAACATTGGACAAGCCATATGTTCTTCAAACAAATGTGTTTATGAGAGGGAGTGGAGATGGAAATATTATAGGGAGGGAAATGAGGTTCCATCTTTGGTTTGACCCAACTCAAGATTTTCACAATTACGCCATTTTATGGACTCCAGAAGAAATCATATTCTTAGTGGACGACGTGCCAATCAGGAGGTACGAAAGGAAGAGCGACGCAACATTTCCAGTGAGGCCAATGTGGGTTTACGGGTCAATATGGGATGCATCATCATGGGCAACAGAGGACGGAAAATACAAAGCTGATTACAAATACCAACCGTTTATCGGAAGATACAACAACTTCAAGCTAGGTGGGTGTGCGGCAGGCGGGGCAGCATCATGTCGTCCGCTCAACAGCGGACCAGGCGGAGGTGGTCGCAGCGGGATGAGCCAGCAGCAGGAAAAGTCCATGGAGTGGGTGCAAAGCAATTATTTGGTGTACAACTACTGTCATGATCCAAGAAGAGATCACACTCTCACTCCAGAGTGTTGA

Protein sequence

MAHYFLILLSLFLFFSFTSCQNPPSPGYFPSNQVHSISFDQGFRNRWGSQHQKVDQRTLTIWLDSSSGIFVDWSGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGNHDEIDIEFLGTTLDKPYVLQTNVFMRGSGDGNIIGREMRFHLWFDPTQDFHNYAILWTPEEIIFLVDDVPIRRYERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCAAGGAASCRPLNSGPGGGGRSGMSQQQEKSMEWVQSNYLVYNYCHDPRRDHTLTPEC

Homology

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BLAST of Lsi03G000150 vs. Swiss-Prot
Match: XTH32_ARATH (Probable xyloglucan endotransglucosylase/hydrolase protein 32 OS=Arabidopsis thaliana GN=XTH32 PE=2 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 4.1e-116
Identity = 198/304 (65.13%), Postives = 242/304 (79.61%), Query Frame = 1


Query: 1   MAHYFLILLSLF----LFFSFTSCQNPPSPGYFPSNQVHSISFDQGFRNRWGSQHQKVDQ 60
           M +  + LLS+F    L+ S  +   PPSPGY+PS++V S++F +GFRN WG QHQ++DQ
Sbjct: 1   MGNSLISLLSIFHLLVLWGSSVNAYWPPSPGYWPSSKVGSLNFYKGFRNLWGPQHQRMDQ 60

Query: 61  RTLTIWLDSSSGIFVDWSGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGN 120
             LTIWLD +SG     SGFKS+  ++SGYFGA IKL PGYTAGVITS YLSNNE +PG 
Sbjct: 61  NALTIWLDRTSG-----SGFKSVKPFRSGYFGANIKLQPGYTAGVITSLYLSNNEAHPGF 120

Query: 121 HDEIDIEFLGTTLDKPYVLQTNVFMRGSGDGNIIGREMRFHLWFDPTQDFHNYAILWTPE 180
           HDE+DIEFLGTT  KPY LQTNV++RGSGDG IIGREM+F LWFDPT+DFH+YAILW+P 
Sbjct: 121 HDEVDIEFLGTTFGKPYTLQTNVYIRGSGDGKIIGREMKFRLWFDPTKDFHHYAILWSPR 180

Query: 181 EIIFLVDDVPIRRYERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYN 240
           EIIFLVDD+PIRRY +KS +TFP+RPMW+YGSIWDASSWATEDGKYKADYKYQPF  +Y 
Sbjct: 181 EIIFLVDDIPIRRYPKKSASTFPLRPMWLYGSIWDASSWATEDGKYKADYKYQPFTAKYT 240

Query: 241 NFKLGGCAAGGAASCRPLNSGPGGGGRSGMSQQQEKSMEWVQSNYLVYNYCHDPRRDHTL 300
           NFK  GC A  +A C PL++ P   G  G+++QQ ++M WVQ++ +VYNYC D +RDH+L
Sbjct: 241 NFKALGCTAYSSARCYPLSASPYRSG--GLTRQQHQAMRWVQTHSMVYNYCKDYKRDHSL 297

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BLAST of Lsi03G000150 vs. Swiss-Prot
Match: XTH31_ARATH (Xyloglucan endotransglucosylase/hydrolase protein 31 OS=Arabidopsis thaliana GN=XTH31 PE=1 SV=2)

HSP 1 Score: 406.4 bits (1043), Expect = 2.8e-112
Identity = 189/300 (63.00%), Postives = 229/300 (76.33%), Query Frame = 1


Query: 1   MAHYFLILLSLFLFFSFTSCQNPPSPGYFPSNQVHSISFDQGFRNRWGSQHQKVDQRTLT 60
           MA   + L  L L  S    Q  PSPGY+PS++V +  FD+ FR  WGSQHQ+ +Q  +T
Sbjct: 1   MALSLIFLALLVLCPSSGHSQRSPSPGYYPSSRVPTSPFDREFRTLWGSQHQRREQDVVT 60

Query: 61  IWLDSSSGIFVDWSGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGNHDEI 120
           +WLD S+G     SGFKSL  Y+SGYFGA+IKL PG+TAGV TS YLSNN+++PG+HDE+
Sbjct: 61  LWLDKSTG-----SGFKSLRPYRSGYFGASIKLQPGFTAGVDTSLYLSNNQEHPGDHDEV 120

Query: 121 DIEFLGTTLDKPYVLQTNVFMRGSGDGNIIGREMRFHLWFDPTQDFHNYAILWTPEEIIF 180
           DIEFLGTT  KPY LQTNVF+RGSGD N+IGREM+F LWFDPTQDFH+YAILW P +I+F
Sbjct: 121 DIEFLGTTPGKPYSLQTNVFVRGSGDRNVIGREMKFTLWFDPTQDFHHYAILWNPNQIVF 180

Query: 181 LVDDVPIRRYERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKL 240
            VDDVPIR Y RK++A FP RPMWVYGSIWDAS WATE+G+ KADY+YQPF+ +Y NFKL
Sbjct: 181 FVDDVPIRTYNRKNEAIFPTRPMWVYGSIWDASDWATENGRIKADYRYQPFVAKYKNFKL 240

Query: 241 GGCAAGGAASCRPLNSGPGGGGRSGMSQQQEKSMEWVQSNYLVYNYCHDPRRDHTLTPEC 300
            GC A  ++SCRP    P      G+S+QQ  ++ W Q N+LVYNYCHDP+RDHT TPEC
Sbjct: 241 AGCTADSSSSCRP--PSPAPMRNRGLSRQQMAALTWAQRNFLVYNYCHDPKRDHTQTPEC 293

[Close]

BLAST of Lsi03G000150 vs. Swiss-Prot
Match: XTH33_ARATH (Probable xyloglucan endotransglucosylase/hydrolase protein 33 OS=Arabidopsis thaliana GN=XTH33 PE=2 SV=2)

HSP 1 Score: 208.4 bits (529), Expect = 1.1e-52
Identity = 106/286 (37.06%), Postives = 162/286 (56.64%), Query Frame = 1


Query: 31  SNQVHSISFDQGFRNRWGSQHQKVDQRTLTIWLDSSSGIFVDWSGFKSLHRYQSGYFGAA 90
           ++Q   I+ + GF  R+G+ + +V+     + LD SSG     +G  S ++Y  G+F A 
Sbjct: 35  TDQFSKIAIENGFSRRFGAHNIQVNGSLAKLTLDKSSG-----AGLVSKNKYHYGFFSAR 94

Query: 91  IKLHPGYTAGVITSFYLSNNEDYPGNHDEIDIEFLGTTLDKPYVLQTNVFMRGSGDGNII 150
           +KL  G+ +GV+ +FYLSN E YP +HDEIDIE LG +    + +QTNV+  GS      
Sbjct: 95  LKLPAGFASGVVVAFYLSNAETYPKSHDEIDIELLGRSRRDDWTIQTNVYANGS---TRT 154

Query: 151 GREMRFHLWFDPTQDFHNYAILWTPEEIIFLVDDVPIRRYERKSDAT--FPVRPMWVYGS 210
           GRE +F+ WFDPTQ FH+Y ++W     +FLVD++P+R++  +   T  +P +PM +Y +
Sbjct: 155 GREEKFYFWFDPTQAFHDYTLIWNSHHTVFLVDNIPVRQFPNRGAFTSAYPSKPMSLYVT 214

Query: 211 IWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCAAGGAASCRPLNSGP---GGGGRSG 270
           +WD S WAT+ GKY  +YKY PF+    + +L GC+    +S     SGP    GG  S 
Sbjct: 215 VWDGSEWATKGGKYPVNYKYAPFVVSVADVELSGCSVNNGSS---TGSGPCTKSGGSISS 274

Query: 271 -----------MSQQQEKSMEWVQSNYLVYNYCHDPRRDHTLTPEC 301
                      +S+ Q  +M+W +   + Y+YC D  R   +  EC
Sbjct: 275 LDPVDGQDFATLSKNQINAMDWARRKLMFYSYCSDKPRYKVMPAEC 309

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BLAST of Lsi03G000150 vs. Swiss-Prot
Match: XTH29_ARATH (Probable xyloglucan endotransglucosylase/hydrolase protein 29 OS=Arabidopsis thaliana GN=XTH29 PE=2 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 2.5e-52
Identity = 111/286 (38.81%), Postives = 160/286 (55.94%), Query Frame = 1


Query: 34  VHSISFDQGFRNRWGSQH--QKVDQRTLTIWLDSSSGIFVDWSGFKSLHRYQSGYFGAAI 93
           ++ I FD+G  + +G  +  +  D R++ + LD  +G     SGF S   YQ G+F + I
Sbjct: 35  INPIFFDEGLSHLFGEGNLIRSPDDRSVRLLLDKYTG-----SGFISSSMYQHGFFSSLI 94

Query: 94  KLHPGYTAGVITSFYLSNNEDYPGNHDEIDIEFLGTTLDKPYVLQTNVFMRGSGDGNIIG 153
           KL   YTAG++ +FY SN + +  +HDE+DIEFLG    KP+  QTN++  GS +    G
Sbjct: 95  KLPGAYTAGIVVAFYTSNGDVFVKDHDELDIEFLGNLEGKPWRFQTNMYGNGSTNR---G 154

Query: 154 REMRFHLWFDPTQDFHNYAILWTPEEIIFLVDDVPIRRYERKSD--ATFPVRPMWVYGSI 213
           RE R+ LWFDP+++FH Y+ILWTP +IIF VDDVPIR   RK +    +P +PM +Y +I
Sbjct: 155 REERYRLWFDPSKEFHRYSILWTPTKIIFWVDDVPIREILRKEEMNGDYPQKPMSLYATI 214

Query: 214 WDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCAAGGAASCRPLNSGPG---------- 273
           WDASSWAT  GK+  DY + PF+  + +  L GC    +      N+  G          
Sbjct: 215 WDASSWATSGGKFGVDYTFSPFVSEFKDIALDGCNVSDSFPGENNNNNIGNYNNINCSVS 274

Query: 274 -----GGGRSGMSQQQEKSMEWVQSNYLVYNYCHDPRRDHTLTPEC 301
                    S +S +Q  +M   +  Y+ Y+YC+D  R     PEC
Sbjct: 275 DQFLMSNDYSTISPKQATAMRRFRERYMYYSYCYDTIRYSVPPPEC 312

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BLAST of Lsi03G000150 vs. Swiss-Prot
Match: XTH28_ARATH (Probable xyloglucan endotransglucosylase/hydrolase protein 28 OS=Arabidopsis thaliana GN=XTH28 PE=2 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.2e-51
Identity = 117/303 (38.61%), Postives = 173/303 (57.10%), Query Frame = 1


Query: 5   FLILLSLFLFFSFTSCQNPPSPGYFPSNQVHSISFDQGFRNRWGSQHQKV--DQRTLTIW 64
           FL+ +SLF     TS  +      F   ++  I FD+G+   +G Q+  V  D +++ + 
Sbjct: 7   FLVFMSLF-----TSLVSG-----FALQKLPLIQFDEGYTQLFGDQNLIVHRDGKSVRLT 66

Query: 65  LDSSSGIFVDWSGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGNHDEIDI 124
           LD  +G     SGF S   Y  G+F ++IKL   Y+AGV+ +FYLSN + Y  NHDEID 
Sbjct: 67  LDERTG-----SGFVSNDIYLHGFFSSSIKLPADYSAGVVIAFYLSNGDLYEKNHDEIDF 126

Query: 125 EFLGTTLDKPYVLQTNVFMRGSGDGNIIGREMRFHLWFDPTQDFHNYAILWTPEEIIFLV 184
           EFLG    + + +QTN++  GS     +GRE R++LWFDPT+DFH Y+ILW+   IIF V
Sbjct: 127 EFLGNIRGREWRIQTNIYGNGS---THLGREERYNLWFDPTEDFHQYSILWSLSHIIFYV 186

Query: 185 DDVPIRRYERKSD--ATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKL 244
           D+VPIR  +R +     FP +PM +Y +IWD S WAT+ GKY  +YKY P++ ++ +  L
Sbjct: 187 DNVPIREVKRTASMGGDFPAKPMSLYSTIWDGSKWATDGGKYGVNYKYAPYVSQFTDLIL 246

Query: 245 GGCAAGGA---ASCRPLNSGPGGGGRSGMSQQQEKSMEWVQSNYLVYNYCHDPRRDHTLT 301
            GCA        SC+   +       S +++ Q   ME  +  ++ Y+YC+D  R   + 
Sbjct: 247 HGCAVDPTEKFPSCKD-EAVQNLRLASEITESQRNKMEIFRQKHMTYSYCYDHMRYKVVL 290

[Close]

BLAST of Lsi03G000150 vs. TrEMBL
Match: A0A0A0LV02_CUCSA (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus GN=Csa_1G109370 PE=3 SV=1)

HSP 1 Score: 567.8 bits (1462), Expect = 8.0e-159
Identity = 274/304 (90.13%), Postives = 279/304 (91.78%), Query Frame = 1


Query: 1   MAHYF-LILLSLFLFF--SFTSCQN-PPSPGYFPSNQVHSISFDQGFRNRWGSQHQKVDQ 60
           M+HYF L LLSLF FF  SFTS QN PPSPGYFPS QV S  FDQ FRNRWGSQHQKVDQ
Sbjct: 1   MSHYFFLFLLSLFFFFFFSFTSSQNYPPSPGYFPSTQVQSTGFDQVFRNRWGSQHQKVDQ 60

Query: 61  RTLTIWLDSSSGIFVDWSGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGN 120
            TLTIWLDSSSG     SGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGN
Sbjct: 61  GTLTIWLDSSSG-----SGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGN 120

Query: 121 HDEIDIEFLGTTLDKPYVLQTNVFMRGSGDGNIIGREMRFHLWFDPTQDFHNYAILWTPE 180
           HDEIDIEFLGTT DKPYVLQTNVFMRGSGDGNIIGREMRFHLWF+PTQDFHNYAILWTPE
Sbjct: 121 HDEIDIEFLGTTSDKPYVLQTNVFMRGSGDGNIIGREMRFHLWFNPTQDFHNYAILWTPE 180

Query: 181 EIIFLVDDVPIRRYERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYN 240
           EIIFLVDDVPIRRYERKS+ATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYN
Sbjct: 181 EIIFLVDDVPIRRYERKSEATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYN 240

Query: 241 NFKLGGCAAGGAASCRPLNSGPGGGGRSGMSQQQEKSMEWVQSNYLVYNYCHDPRRDHTL 300
           NFKL GC   GAASCRPLNSGPGGGGR  MSQQQEK+MEWVQ+NYLVYNYCHDPRRDHTL
Sbjct: 241 NFKLSGCTTDGAASCRPLNSGPGGGGRGRMSQQQEKAMEWVQNNYLVYNYCHDPRRDHTL 299

[Close]

BLAST of Lsi03G000150 vs. TrEMBL
Match: N0DXB5_CUCSA (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus GN=xth23 PE=3 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 5.0e-145
Identity = 252/301 (83.72%), Postives = 262/301 (87.04%), Query Frame = 1


Query: 1   MAHYFLILLSLFLFFSFT-SCQNPPSPGYFPSNQVHSISFDQGFRNRWGSQHQKVDQRTL 60
           M+HYF        FFSF+ S  +  S    P   +  ++  Q FRNRWGSQHQKVDQ TL
Sbjct: 1   MSHYF--------FFSFSLSSSSSSSLSPLPKTILPPLAIFQVFRNRWGSQHQKVDQGTL 60

Query: 61  TIWLDSSSGIFVDWSGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGNHDE 120
           TIWLDSSSG     SGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGNHDE
Sbjct: 61  TIWLDSSSG-----SGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGNHDE 120

Query: 121 IDIEFLGTTLDKPYVLQTNVFMRGSGDGNIIGREMRFHLWFDPTQDFHNYAILWTPEEII 180
           IDIEFLGTT DKPYVLQTNVFMRGSGDGNIIGREMRFHLWF+PTQDFHNYAILWTPEEII
Sbjct: 121 IDIEFLGTTSDKPYVLQTNVFMRGSGDGNIIGREMRFHLWFNPTQDFHNYAILWTPEEII 180

Query: 181 FLVDDVPIRRYERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFK 240
           FLVDDVPIRRYERKS+ATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFK
Sbjct: 181 FLVDDVPIRRYERKSEATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFK 240

Query: 241 LGGCAAGGAASCRPLNSGPGGGGRSGMSQQQEKSMEWVQSNYLVYNYCHDPRRDHTLTPE 300
           L GC   GAASCRPLNSGPG GGR  MSQQQEK+MEWVQ+NYLVYNYCHDPRRDHTLTPE
Sbjct: 241 LSGCTTDGAASCRPLNSGPGRGGRGRMSQQQEKAMEWVQNNYLVYNYCHDPRRDHTLTPE 288

[Close]

BLAST of Lsi03G000150 vs. TrEMBL
Match: A0A067L9Q6_JATCU (Xyloglucan endotransglucosylase/hydrolase OS=Jatropha curcas GN=JCGZ_05445 PE=3 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 2.2e-132
Identity = 221/296 (74.66%), Postives = 250/296 (84.46%), Query Frame = 1


Query: 5   FLILLSLFLFFSFTSCQNPPSPGYFPSNQVHSISFDQGFRNRWGSQHQKVDQRTLTIWLD 64
           +L+ L+L +F   +S Q+PPSPGY PS+++  I F+QGFRN WG QHQK+DQ TLT+WLD
Sbjct: 6   YLLPLTLIIFSGSSSAQSPPSPGYSPSSRISPIGFNQGFRNLWGPQHQKIDQGTLTVWLD 65

Query: 65  SSSGIFVDWSGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGNHDEIDIEF 124
           SSSG     SG+KSL  Y+SGYFGAA+KLHPGYTAGVITSFYLSNNED+PGNHDEIDIEF
Sbjct: 66  SSSG-----SGYKSLQSYRSGYFGAAMKLHPGYTAGVITSFYLSNNEDFPGNHDEIDIEF 125

Query: 125 LGTTLDKPYVLQTNVFMRGSGDGNIIGREMRFHLWFDPTQDFHNYAILWTPEEIIFLVDD 184
           LGTT  KPY LQTNV+M+GSGDG IIGREMRFHLWFDPTQDFHNYAILWTP EIIF VDD
Sbjct: 126 LGTTPGKPYTLQTNVYMKGSGDGKIIGREMRFHLWFDPTQDFHNYAILWTPNEIIFFVDD 185

Query: 185 VPIRRYERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCA 244
           VPIRRY RK+DATFP+RPMWVYGSIWDASSWATEDGKYKADY YQPFIGRY NFK+ GC+
Sbjct: 186 VPIRRYPRKNDATFPLRPMWVYGSIWDASSWATEDGKYKADYSYQPFIGRYRNFKISGCS 245

Query: 245 AGGAASCRPLNSGPGGGGRSGMSQQQEKSMEWVQSNYLVYNYCHDPRRDHTLTPEC 301
             G ASC   ++ P G   SG+SQQQ  +M+WVQSNYL Y+YC DP+RDHT TPEC
Sbjct: 246 TSGPASCHSPSASPSG---SGLSQQQYSAMQWVQSNYLAYDYCTDPKRDHTQTPEC 293

[Close]

BLAST of Lsi03G000150 vs. TrEMBL
Match: A0A061H0B3_THECC (Xyloglucan endotransglucosylase/hydrolase OS=Theobroma cacao GN=TCM_042151 PE=3 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 1.9e-131
Identity = 225/300 (75.00%), Postives = 247/300 (82.33%), Query Frame = 1


Query: 1   MAHYFLILLSLFLFFSFTSCQNPPSPGYFPSNQVHSISFDQGFRNRWGSQHQKVDQRTLT 60
           MA  F + L+ FL  S  + Q PPSPGY PS++V S+ FDQGFRN WG QHQ++DQ +LT
Sbjct: 65  MAPIFSLFLA-FLIISSGNAQGPPSPGYSPSSRVSSVDFDQGFRNLWGPQHQRIDQGSLT 124

Query: 61  IWLDSSSGIFVDWSGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGNHDEI 120
           IWLD SSG     SGFKSL  YQSGYFGAAIKL PGYTAGVITSFYLSNNED+PGNHDEI
Sbjct: 125 IWLDKSSG-----SGFKSLRPYQSGYFGAAIKLQPGYTAGVITSFYLSNNEDHPGNHDEI 184

Query: 121 DIEFLGTTLDKPYVLQTNVFMRGSGDGNIIGREMRFHLWFDPTQDFHNYAILWTPEEIIF 180
           DIEFLGTT DKPY LQTNV++RG+GDGNIIGREMRFHLWFDPTQD+HNYAILWTP EI+F
Sbjct: 185 DIEFLGTTPDKPYTLQTNVYIRGTGDGNIIGREMRFHLWFDPTQDYHNYAILWTPSEIMF 244

Query: 181 LVDDVPIRRYERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKL 240
            VDDVPIRRY RKSDATFP RPMWVYGSIWDASSWATE+GKYKADY YQPFI RY NFKL
Sbjct: 245 FVDDVPIRRYPRKSDATFPTRPMWVYGSIWDASSWATENGKYKADYNYQPFIARYTNFKL 304

Query: 241 GGCAAGGAASCRPLNSGPGGGGRSGMSQQQEKSMEWVQSNYLVYNYCHDPRRDHTLTPEC 300
            GC A G  SCRP ++ P G    G+SQQQ  +M WVQ NYLVY+YCHD +RDHT TPEC
Sbjct: 305 SGCNADGPTSCRPPSASPSGS--DGLSQQQASAMNWVQGNYLVYDYCHDSKRDHTQTPEC 356

[Close]

BLAST of Lsi03G000150 vs. TrEMBL
Match: A9PG67_POPTR (Xyloglucan endotransglucosylase/hydrolase OS=Populus trichocarpa GN=POPTR_0009s01210g PE=2 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 9.2e-131
Identity = 221/296 (74.66%), Postives = 249/296 (84.12%), Query Frame = 1


Query: 5   FLILLSLFLFFSFTSCQNPPSPGYFPSNQVHSISFDQGFRNRWGSQHQKVDQRTLTIWLD 64
           +L L+ L +    T+ QN PSPGY+P +++ SI FDQG+ N WG+QHQ+V+Q T+TIWLD
Sbjct: 6   YLPLILLIIPLMITTAQNTPSPGYYPGSRISSIGFDQGYSNLWGAQHQRVEQGTVTIWLD 65

Query: 65  SSSGIFVDWSGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGNHDEIDIEF 124
           SSSG     SG+KSLH Y+SGYFGAAIKL PGYTAGVITSFYLSNNE +PG+HDEIDIEF
Sbjct: 66  SSSG-----SGYKSLHPYRSGYFGAAIKLQPGYTAGVITSFYLSNNEAHPGDHDEIDIEF 125

Query: 125 LGTTLDKPYVLQTNVFMRGSGDGNIIGREMRFHLWFDPTQDFHNYAILWTPEEIIFLVDD 184
           LGTT DKPY LQTNV++RGSGD NIIGREM+FHLWFDPTQDFHNYAILWTP EIIFLVDD
Sbjct: 126 LGTTPDKPYTLQTNVYIRGSGDRNIIGREMKFHLWFDPTQDFHNYAILWTPSEIIFLVDD 185

Query: 185 VPIRRYERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCA 244
           VPIRRY RKSDATFP+RPMWVYGSIWDASSWATEDGKYKADY YQPFIGRY NFK+GGC 
Sbjct: 186 VPIRRYPRKSDATFPLRPMWVYGSIWDASSWATEDGKYKADYNYQPFIGRYKNFKIGGCT 245

Query: 245 AGGAASCRPLNSGPGGGGRSGMSQQQEKSMEWVQSNYLVYNYCHDPRRDHTLTPEC 301
           A G A+C P ++ P G G  G+SQQQ   MEWVQ NYLVY+YC D +RDHT TPEC
Sbjct: 246 ADGPAACSPPSASPSGSG--GLSQQQSSVMEWVQRNYLVYDYCRDGKRDHTQTPEC 294

[Close]

BLAST of Lsi03G000150 vs. TAIR10
Match: AT2G36870.1 (AT2G36870.1 xyloglucan endotransglucosylase/hydrolase 32)

HSP 1 Score: 419.1 bits (1076), Expect = 2.3e-117
Identity = 198/304 (65.13%), Postives = 242/304 (79.61%), Query Frame = 1


Query: 1   MAHYFLILLSLF----LFFSFTSCQNPPSPGYFPSNQVHSISFDQGFRNRWGSQHQKVDQ 60
           M +  + LLS+F    L+ S  +   PPSPGY+PS++V S++F +GFRN WG QHQ++DQ
Sbjct: 1   MGNSLISLLSIFHLLVLWGSSVNAYWPPSPGYWPSSKVGSLNFYKGFRNLWGPQHQRMDQ 60

Query: 61  RTLTIWLDSSSGIFVDWSGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGN 120
             LTIWLD +SG     SGFKS+  ++SGYFGA IKL PGYTAGVITS YLSNNE +PG 
Sbjct: 61  NALTIWLDRTSG-----SGFKSVKPFRSGYFGANIKLQPGYTAGVITSLYLSNNEAHPGF 120

Query: 121 HDEIDIEFLGTTLDKPYVLQTNVFMRGSGDGNIIGREMRFHLWFDPTQDFHNYAILWTPE 180
           HDE+DIEFLGTT  KPY LQTNV++RGSGDG IIGREM+F LWFDPT+DFH+YAILW+P 
Sbjct: 121 HDEVDIEFLGTTFGKPYTLQTNVYIRGSGDGKIIGREMKFRLWFDPTKDFHHYAILWSPR 180

Query: 181 EIIFLVDDVPIRRYERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYN 240
           EIIFLVDD+PIRRY +KS +TFP+RPMW+YGSIWDASSWATEDGKYKADYKYQPF  +Y 
Sbjct: 181 EIIFLVDDIPIRRYPKKSASTFPLRPMWLYGSIWDASSWATEDGKYKADYKYQPFTAKYT 240

Query: 241 NFKLGGCAAGGAASCRPLNSGPGGGGRSGMSQQQEKSMEWVQSNYLVYNYCHDPRRDHTL 300
           NFK  GC A  +A C PL++ P   G  G+++QQ ++M WVQ++ +VYNYC D +RDH+L
Sbjct: 241 NFKALGCTAYSSARCYPLSASPYRSG--GLTRQQHQAMRWVQTHSMVYNYCKDYKRDHSL 297

[Close]

BLAST of Lsi03G000150 vs. TAIR10
Match: AT3G44990.1 (AT3G44990.1 xyloglucan endo-transglycosylase-related 8)

HSP 1 Score: 406.4 bits (1043), Expect = 1.6e-113
Identity = 189/300 (63.00%), Postives = 229/300 (76.33%), Query Frame = 1


Query: 1   MAHYFLILLSLFLFFSFTSCQNPPSPGYFPSNQVHSISFDQGFRNRWGSQHQKVDQRTLT 60
           MA   + L  L L  S    Q  PSPGY+PS++V +  FD+ FR  WGSQHQ+ +Q  +T
Sbjct: 1   MALSLIFLALLVLCPSSGHSQRSPSPGYYPSSRVPTSPFDREFRTLWGSQHQRREQDVVT 60

Query: 61  IWLDSSSGIFVDWSGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGNHDEI 120
           +WLD S+G     SGFKSL  Y+SGYFGA+IKL PG+TAGV TS YLSNN+++PG+HDE+
Sbjct: 61  LWLDKSTG-----SGFKSLRPYRSGYFGASIKLQPGFTAGVDTSLYLSNNQEHPGDHDEV 120

Query: 121 DIEFLGTTLDKPYVLQTNVFMRGSGDGNIIGREMRFHLWFDPTQDFHNYAILWTPEEIIF 180
           DIEFLGTT  KPY LQTNVF+RGSGD N+IGREM+F LWFDPTQDFH+YAILW P +I+F
Sbjct: 121 DIEFLGTTPGKPYSLQTNVFVRGSGDRNVIGREMKFTLWFDPTQDFHHYAILWNPNQIVF 180

Query: 181 LVDDVPIRRYERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKL 240
            VDDVPIR Y RK++A FP RPMWVYGSIWDAS WATE+G+ KADY+YQPF+ +Y NFKL
Sbjct: 181 FVDDVPIRTYNRKNEAIFPTRPMWVYGSIWDASDWATENGRIKADYRYQPFVAKYKNFKL 240

Query: 241 GGCAAGGAASCRPLNSGPGGGGRSGMSQQQEKSMEWVQSNYLVYNYCHDPRRDHTLTPEC 300
            GC A  ++SCRP    P      G+S+QQ  ++ W Q N+LVYNYCHDP+RDHT TPEC
Sbjct: 241 AGCTADSSSSCRP--PSPAPMRNRGLSRQQMAALTWAQRNFLVYNYCHDPKRDHTQTPEC 293

[Close]

BLAST of Lsi03G000150 vs. TAIR10
Match: AT1G10550.1 (AT1G10550.1 xyloglucan:xyloglucosyl transferase 33)

HSP 1 Score: 208.4 bits (529), Expect = 6.2e-54
Identity = 106/286 (37.06%), Postives = 162/286 (56.64%), Query Frame = 1


Query: 31  SNQVHSISFDQGFRNRWGSQHQKVDQRTLTIWLDSSSGIFVDWSGFKSLHRYQSGYFGAA 90
           ++Q   I+ + GF  R+G+ + +V+     + LD SSG     +G  S ++Y  G+F A 
Sbjct: 35  TDQFSKIAIENGFSRRFGAHNIQVNGSLAKLTLDKSSG-----AGLVSKNKYHYGFFSAR 94

Query: 91  IKLHPGYTAGVITSFYLSNNEDYPGNHDEIDIEFLGTTLDKPYVLQTNVFMRGSGDGNII 150
           +KL  G+ +GV+ +FYLSN E YP +HDEIDIE LG +    + +QTNV+  GS      
Sbjct: 95  LKLPAGFASGVVVAFYLSNAETYPKSHDEIDIELLGRSRRDDWTIQTNVYANGS---TRT 154

Query: 151 GREMRFHLWFDPTQDFHNYAILWTPEEIIFLVDDVPIRRYERKSDAT--FPVRPMWVYGS 210
           GRE +F+ WFDPTQ FH+Y ++W     +FLVD++P+R++  +   T  +P +PM +Y +
Sbjct: 155 GREEKFYFWFDPTQAFHDYTLIWNSHHTVFLVDNIPVRQFPNRGAFTSAYPSKPMSLYVT 214

Query: 211 IWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCAAGGAASCRPLNSGP---GGGGRSG 270
           +WD S WAT+ GKY  +YKY PF+    + +L GC+    +S     SGP    GG  S 
Sbjct: 215 VWDGSEWATKGGKYPVNYKYAPFVVSVADVELSGCSVNNGSS---TGSGPCTKSGGSISS 274

Query: 271 -----------MSQQQEKSMEWVQSNYLVYNYCHDPRRDHTLTPEC 301
                      +S+ Q  +M+W +   + Y+YC D  R   +  EC
Sbjct: 275 LDPVDGQDFATLSKNQINAMDWARRKLMFYSYCSDKPRYKVMPAEC 309

[Close]

BLAST of Lsi03G000150 vs. TAIR10
Match: AT4G18990.1 (AT4G18990.1 xyloglucan endotransglucosylase/hydrolase 29)

HSP 1 Score: 207.2 bits (526), Expect = 1.4e-53
Identity = 111/286 (38.81%), Postives = 160/286 (55.94%), Query Frame = 1


Query: 34  VHSISFDQGFRNRWGSQH--QKVDQRTLTIWLDSSSGIFVDWSGFKSLHRYQSGYFGAAI 93
           ++ I FD+G  + +G  +  +  D R++ + LD  +G     SGF S   YQ G+F + I
Sbjct: 35  INPIFFDEGLSHLFGEGNLIRSPDDRSVRLLLDKYTG-----SGFISSSMYQHGFFSSLI 94

Query: 94  KLHPGYTAGVITSFYLSNNEDYPGNHDEIDIEFLGTTLDKPYVLQTNVFMRGSGDGNIIG 153
           KL   YTAG++ +FY SN + +  +HDE+DIEFLG    KP+  QTN++  GS +    G
Sbjct: 95  KLPGAYTAGIVVAFYTSNGDVFVKDHDELDIEFLGNLEGKPWRFQTNMYGNGSTNR---G 154

Query: 154 REMRFHLWFDPTQDFHNYAILWTPEEIIFLVDDVPIRRYERKSD--ATFPVRPMWVYGSI 213
           RE R+ LWFDP+++FH Y+ILWTP +IIF VDDVPIR   RK +    +P +PM +Y +I
Sbjct: 155 REERYRLWFDPSKEFHRYSILWTPTKIIFWVDDVPIREILRKEEMNGDYPQKPMSLYATI 214

Query: 214 WDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCAAGGAASCRPLNSGPG---------- 273
           WDASSWAT  GK+  DY + PF+  + +  L GC    +      N+  G          
Sbjct: 215 WDASSWATSGGKFGVDYTFSPFVSEFKDIALDGCNVSDSFPGENNNNNIGNYNNINCSVS 274

Query: 274 -----GGGRSGMSQQQEKSMEWVQSNYLVYNYCHDPRRDHTLTPEC 301
                    S +S +Q  +M   +  Y+ Y+YC+D  R     PEC
Sbjct: 275 DQFLMSNDYSTISPKQATAMRRFRERYMYYSYCYDTIRYSVPPPEC 312

[Close]

BLAST of Lsi03G000150 vs. TAIR10
Match: AT1G14720.1 (AT1G14720.1 xyloglucan endotransglucosylase/hydrolase 28)

HSP 1 Score: 204.9 bits (520), Expect = 6.9e-53
Identity = 117/303 (38.61%), Postives = 173/303 (57.10%), Query Frame = 1


Query: 5   FLILLSLFLFFSFTSCQNPPSPGYFPSNQVHSISFDQGFRNRWGSQHQKV--DQRTLTIW 64
           FL+ +SLF     TS  +      F   ++  I FD+G+   +G Q+  V  D +++ + 
Sbjct: 7   FLVFMSLF-----TSLVSG-----FALQKLPLIQFDEGYTQLFGDQNLIVHRDGKSVRLT 66

Query: 65  LDSSSGIFVDWSGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGNHDEIDI 124
           LD  +G     SGF S   Y  G+F ++IKL   Y+AGV+ +FYLSN + Y  NHDEID 
Sbjct: 67  LDERTG-----SGFVSNDIYLHGFFSSSIKLPADYSAGVVIAFYLSNGDLYEKNHDEIDF 126

Query: 125 EFLGTTLDKPYVLQTNVFMRGSGDGNIIGREMRFHLWFDPTQDFHNYAILWTPEEIIFLV 184
           EFLG    + + +QTN++  GS     +GRE R++LWFDPT+DFH Y+ILW+   IIF V
Sbjct: 127 EFLGNIRGREWRIQTNIYGNGS---THLGREERYNLWFDPTEDFHQYSILWSLSHIIFYV 186

Query: 185 DDVPIRRYERKSD--ATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKL 244
           D+VPIR  +R +     FP +PM +Y +IWD S WAT+ GKY  +YKY P++ ++ +  L
Sbjct: 187 DNVPIREVKRTASMGGDFPAKPMSLYSTIWDGSKWATDGGKYGVNYKYAPYVSQFTDLIL 246

Query: 245 GGCAAGGA---ASCRPLNSGPGGGGRSGMSQQQEKSMEWVQSNYLVYNYCHDPRRDHTLT 301
            GCA        SC+   +       S +++ Q   ME  +  ++ Y+YC+D  R   + 
Sbjct: 247 HGCAVDPTEKFPSCKD-EAVQNLRLASEITESQRNKMEIFRQKHMTYSYCYDHMRYKVVL 290

[Close]

BLAST of Lsi03G000150 vs. NCBI nr
Match: gi|659071993|ref|XP_008462950.1| (PREDICTED: probable xyloglucan endotransglucosylase/hydrolase protein 32 [Cucumis melo])

HSP 1 Score: 571.2 bits (1471), Expect = 1.0e-159
Identity = 272/300 (90.67%), Postives = 278/300 (92.67%), Query Frame = 1


Query: 2   AHYFLILLSLFLFFSFTSCQN-PPSPGYFPSNQVHSISFDQGFRNRWGSQHQKVDQRTLT 61
           ++YFL LLSLF FFSFTS QN PPSPGYFPSNQV SI F Q FRNRWGSQHQKVDQ TLT
Sbjct: 3   SYYFLFLLSLFFFFSFTSSQNYPPSPGYFPSNQVQSIGFYQLFRNRWGSQHQKVDQGTLT 62

Query: 62  IWLDSSSGIFVDWSGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGNHDEI 121
           IWLDSSSG     SGFKSLHRY+SGYFG AIKLHPGYTAGVITSFYLSNNEDYPGNHDEI
Sbjct: 63  IWLDSSSG-----SGFKSLHRYRSGYFGVAIKLHPGYTAGVITSFYLSNNEDYPGNHDEI 122

Query: 122 DIEFLGTTLDKPYVLQTNVFMRGSGDGNIIGREMRFHLWFDPTQDFHNYAILWTPEEIIF 181
           DIEFLGTT DKPYVLQTNVFMRGSGDGNIIGREMRFHLWFDPTQDFH+YAILWTPEEIIF
Sbjct: 123 DIEFLGTTWDKPYVLQTNVFMRGSGDGNIIGREMRFHLWFDPTQDFHSYAILWTPEEIIF 182

Query: 182 LVDDVPIRRYERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKL 241
           LVDDVPIRRYERKSD TFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRY NFKL
Sbjct: 183 LVDDVPIRRYERKSDTTFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYKNFKL 242

Query: 242 GGCAAGGAASCRPLNSGPGGGGRSGMSQQQEKSMEWVQSNYLVYNYCHDPRRDHTLTPEC 301
            GCAA GAASCRPLNSGPGGGGR  MSQQQEK+MEWVQ+NYLVYNYCHDPRRDHTLTPEC
Sbjct: 243 SGCAAEGAASCRPLNSGPGGGGRGRMSQQQEKAMEWVQNNYLVYNYCHDPRRDHTLTPEC 297

[Close]

BLAST of Lsi03G000150 vs. NCBI nr
Match: gi|778664970|ref|XP_004147295.2| (PREDICTED: probable xyloglucan endotransglucosylase/hydrolase protein 31 [Cucumis sativus])

HSP 1 Score: 567.8 bits (1462), Expect = 1.1e-158
Identity = 274/304 (90.13%), Postives = 279/304 (91.78%), Query Frame = 1


Query: 1   MAHYF-LILLSLFLFF--SFTSCQN-PPSPGYFPSNQVHSISFDQGFRNRWGSQHQKVDQ 60
           M+HYF L LLSLF FF  SFTS QN PPSPGYFPS QV S  FDQ FRNRWGSQHQKVDQ
Sbjct: 1   MSHYFFLFLLSLFFFFFFSFTSSQNYPPSPGYFPSTQVQSTGFDQVFRNRWGSQHQKVDQ 60

Query: 61  RTLTIWLDSSSGIFVDWSGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGN 120
            TLTIWLDSSSG     SGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGN
Sbjct: 61  GTLTIWLDSSSG-----SGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGN 120

Query: 121 HDEIDIEFLGTTLDKPYVLQTNVFMRGSGDGNIIGREMRFHLWFDPTQDFHNYAILWTPE 180
           HDEIDIEFLGTT DKPYVLQTNVFMRGSGDGNIIGREMRFHLWF+PTQDFHNYAILWTPE
Sbjct: 121 HDEIDIEFLGTTSDKPYVLQTNVFMRGSGDGNIIGREMRFHLWFNPTQDFHNYAILWTPE 180

Query: 181 EIIFLVDDVPIRRYERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYN 240
           EIIFLVDDVPIRRYERKS+ATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYN
Sbjct: 181 EIIFLVDDVPIRRYERKSEATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYN 240

Query: 241 NFKLGGCAAGGAASCRPLNSGPGGGGRSGMSQQQEKSMEWVQSNYLVYNYCHDPRRDHTL 300
           NFKL GC   GAASCRPLNSGPGGGGR  MSQQQEK+MEWVQ+NYLVYNYCHDPRRDHTL
Sbjct: 241 NFKLSGCTTDGAASCRPLNSGPGGGGRGRMSQQQEKAMEWVQNNYLVYNYCHDPRRDHTL 299

[Close]

BLAST of Lsi03G000150 vs. NCBI nr
Match: gi|481023330|emb|CCH26633.1| (putative xyloglucan endotransglucosylase/hydrolase [Cucumis sativus])

HSP 1 Score: 521.9 bits (1343), Expect = 7.2e-145
Identity = 252/301 (83.72%), Postives = 262/301 (87.04%), Query Frame = 1


Query: 1   MAHYFLILLSLFLFFSFT-SCQNPPSPGYFPSNQVHSISFDQGFRNRWGSQHQKVDQRTL 60
           M+HYF        FFSF+ S  +  S    P   +  ++  Q FRNRWGSQHQKVDQ TL
Sbjct: 1   MSHYF--------FFSFSLSSSSSSSLSPLPKTILPPLAIFQVFRNRWGSQHQKVDQGTL 60

Query: 61  TIWLDSSSGIFVDWSGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGNHDE 120
           TIWLDSSSG     SGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGNHDE
Sbjct: 61  TIWLDSSSG-----SGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGNHDE 120

Query: 121 IDIEFLGTTLDKPYVLQTNVFMRGSGDGNIIGREMRFHLWFDPTQDFHNYAILWTPEEII 180
           IDIEFLGTT DKPYVLQTNVFMRGSGDGNIIGREMRFHLWF+PTQDFHNYAILWTPEEII
Sbjct: 121 IDIEFLGTTSDKPYVLQTNVFMRGSGDGNIIGREMRFHLWFNPTQDFHNYAILWTPEEII 180

Query: 181 FLVDDVPIRRYERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFK 240
           FLVDDVPIRRYERKS+ATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFK
Sbjct: 181 FLVDDVPIRRYERKSEATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFK 240

Query: 241 LGGCAAGGAASCRPLNSGPGGGGRSGMSQQQEKSMEWVQSNYLVYNYCHDPRRDHTLTPE 300
           L GC   GAASCRPLNSGPG GGR  MSQQQEK+MEWVQ+NYLVYNYCHDPRRDHTLTPE
Sbjct: 241 LSGCTTDGAASCRPLNSGPGRGGRGRMSQQQEKAMEWVQNNYLVYNYCHDPRRDHTLTPE 288

[Close]

BLAST of Lsi03G000150 vs. NCBI nr
Match: gi|802551272|ref|XP_012064710.1| (PREDICTED: probable xyloglucan endotransglucosylase/hydrolase protein 32 [Jatropha curcas])

HSP 1 Score: 479.9 bits (1234), Expect = 3.1e-132
Identity = 221/296 (74.66%), Postives = 250/296 (84.46%), Query Frame = 1


Query: 5   FLILLSLFLFFSFTSCQNPPSPGYFPSNQVHSISFDQGFRNRWGSQHQKVDQRTLTIWLD 64
           +L+ L+L +F   +S Q+PPSPGY PS+++  I F+QGFRN WG QHQK+DQ TLT+WLD
Sbjct: 6   YLLPLTLIIFSGSSSAQSPPSPGYSPSSRISPIGFNQGFRNLWGPQHQKIDQGTLTVWLD 65

Query: 65  SSSGIFVDWSGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGNHDEIDIEF 124
           SSSG     SG+KSL  Y+SGYFGAA+KLHPGYTAGVITSFYLSNNED+PGNHDEIDIEF
Sbjct: 66  SSSG-----SGYKSLQSYRSGYFGAAMKLHPGYTAGVITSFYLSNNEDFPGNHDEIDIEF 125

Query: 125 LGTTLDKPYVLQTNVFMRGSGDGNIIGREMRFHLWFDPTQDFHNYAILWTPEEIIFLVDD 184
           LGTT  KPY LQTNV+M+GSGDG IIGREMRFHLWFDPTQDFHNYAILWTP EIIF VDD
Sbjct: 126 LGTTPGKPYTLQTNVYMKGSGDGKIIGREMRFHLWFDPTQDFHNYAILWTPNEIIFFVDD 185

Query: 185 VPIRRYERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCA 244
           VPIRRY RK+DATFP+RPMWVYGSIWDASSWATEDGKYKADY YQPFIGRY NFK+ GC+
Sbjct: 186 VPIRRYPRKNDATFPLRPMWVYGSIWDASSWATEDGKYKADYSYQPFIGRYRNFKISGCS 245

Query: 245 AGGAASCRPLNSGPGGGGRSGMSQQQEKSMEWVQSNYLVYNYCHDPRRDHTLTPEC 301
             G ASC   ++ P G   SG+SQQQ  +M+WVQSNYL Y+YC DP+RDHT TPEC
Sbjct: 246 TSGPASCHSPSASPSG---SGLSQQQYSAMQWVQSNYLAYDYCTDPKRDHTQTPEC 293

[Close]

BLAST of Lsi03G000150 vs. NCBI nr
Match: gi|590590897|ref|XP_007016865.1| (Xyloglucan endotransglucosylase/hydrolase protein 32 isoform 1 [Theobroma cacao])

HSP 1 Score: 476.9 bits (1226), Expect = 2.7e-131
Identity = 225/300 (75.00%), Postives = 247/300 (82.33%), Query Frame = 1


Query: 1   MAHYFLILLSLFLFFSFTSCQNPPSPGYFPSNQVHSISFDQGFRNRWGSQHQKVDQRTLT 60
           MA  F + L+ FL  S  + Q PPSPGY PS++V S+ FDQGFRN WG QHQ++DQ +LT
Sbjct: 65  MAPIFSLFLA-FLIISSGNAQGPPSPGYSPSSRVSSVDFDQGFRNLWGPQHQRIDQGSLT 124

Query: 61  IWLDSSSGIFVDWSGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGNHDEI 120
           IWLD SSG     SGFKSL  YQSGYFGAAIKL PGYTAGVITSFYLSNNED+PGNHDEI
Sbjct: 125 IWLDKSSG-----SGFKSLRPYQSGYFGAAIKLQPGYTAGVITSFYLSNNEDHPGNHDEI 184

Query: 121 DIEFLGTTLDKPYVLQTNVFMRGSGDGNIIGREMRFHLWFDPTQDFHNYAILWTPEEIIF 180
           DIEFLGTT DKPY LQTNV++RG+GDGNIIGREMRFHLWFDPTQD+HNYAILWTP EI+F
Sbjct: 185 DIEFLGTTPDKPYTLQTNVYIRGTGDGNIIGREMRFHLWFDPTQDYHNYAILWTPSEIMF 244

Query: 181 LVDDVPIRRYERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKL 240
            VDDVPIRRY RKSDATFP RPMWVYGSIWDASSWATE+GKYKADY YQPFI RY NFKL
Sbjct: 245 FVDDVPIRRYPRKSDATFPTRPMWVYGSIWDASSWATENGKYKADYNYQPFIARYTNFKL 304

Query: 241 GGCAAGGAASCRPLNSGPGGGGRSGMSQQQEKSMEWVQSNYLVYNYCHDPRRDHTLTPEC 300
            GC A G  SCRP ++ P G    G+SQQQ  +M WVQ NYLVY+YCHD +RDHT TPEC
Sbjct: 305 SGCNADGPTSCRPPSASPSGS--DGLSQQQASAMNWVQGNYLVYDYCHDSKRDHTQTPEC 356

The following BLAST results are available for this feature:

BLAST of Lsi03G000150 vs. Swiss-Prot


Match Name	E-value	Identity	Description
XTH32_ARATH	4.1e-116	65.13	Probable xyloglucan endotransglucosylase/hydrolase protein 32 OS=Arabidopsis tha...	[more]
XTH31_ARATH	2.8e-112	63.00	Xyloglucan endotransglucosylase/hydrolase protein 31 OS=Arabidopsis thaliana GN=...	[more]
XTH33_ARATH	1.1e-52	37.06	Probable xyloglucan endotransglucosylase/hydrolase protein 33 OS=Arabidopsis tha...	[more]
XTH29_ARATH	2.5e-52	38.81	Probable xyloglucan endotransglucosylase/hydrolase protein 29 OS=Arabidopsis tha...	[more]
XTH28_ARATH	1.2e-51	38.61	Probable xyloglucan endotransglucosylase/hydrolase protein 28 OS=Arabidopsis tha...	[more]

BLAST of Lsi03G000150 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LV02_CUCSA	8.0e-159	90.13	Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus GN=Csa_1G109370 PE=...	[more]
N0DXB5_CUCSA	5.0e-145	83.72	Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus GN=xth23 PE=3 SV=1	[more]
A0A067L9Q6_JATCU	2.2e-132	74.66	Xyloglucan endotransglucosylase/hydrolase OS=Jatropha curcas GN=JCGZ_05445 PE=3 ...	[more]
A0A061H0B3_THECC	1.9e-131	75.00	Xyloglucan endotransglucosylase/hydrolase OS=Theobroma cacao GN=TCM_042151 PE=3 ...	[more]
A9PG67_POPTR	9.2e-131	74.66	Xyloglucan endotransglucosylase/hydrolase OS=Populus trichocarpa GN=POPTR_0009s0...	[more]

BLAST of Lsi03G000150 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G36870.1	2.3e-117	65.13	xyloglucan endotransglucosylase/hydrolase 32	[more]
AT3G44990.1	1.6e-113	63.00	xyloglucan endo-transglycosylase-related 8	[more]
AT1G10550.1	6.2e-54	37.06	xyloglucan:xyloglucosyl transferase 33	[more]
AT4G18990.1	1.4e-53	38.81	xyloglucan endotransglucosylase/hydrolase 29	[more]
AT1G14720.1	6.9e-53	38.61	xyloglucan endotransglucosylase/hydrolase 28	[more]

BLAST of Lsi03G000150 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659071993\|ref\|XP_008462950.1\|	1.0e-159	90.67	PREDICTED: probable xyloglucan endotransglucosylase/hydrolase protein 32 [Cucumi...	[more]
gi\|778664970\|ref\|XP_004147295.2\|	1.1e-158	90.13	PREDICTED: probable xyloglucan endotransglucosylase/hydrolase protein 31 [Cucumi...	[more]
gi\|481023330\|emb\|CCH26633.1\|	7.2e-145	83.72	putative xyloglucan endotransglucosylase/hydrolase [Cucumis sativus]	[more]
gi\|802551272\|ref\|XP_012064710.1\|	3.1e-132	74.66	PREDICTED: probable xyloglucan endotransglucosylase/hydrolase protein 32 [Jatrop...	[more]
gi\|590590897\|ref\|XP_007016865.1\|	2.7e-131	75.00	Xyloglucan endotransglucosylase/hydrolase protein 32 isoform 1 [Theobroma cacao]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0042546	cell wall biogenesis
GO:0010411	xyloglucan metabolic process
GO:0006073	cellular glucan metabolic process
GO:0005975	carbohydrate metabolic process

Vocabulary: **Cellular Component**
Term	Definition
GO:0048046	apoplast
GO:0005618	cell wall

Vocabulary: **Molecular Function**
Term	Definition
GO:0016762	xyloglucan:xyloglucosyl transferase activity
GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds

Vocabulary: **INTERPRO**
Term	Definition
IPR016455	XTH
IPR013320	ConA-like_dom_sf
IPR010713	XET_C
IPR000757	GH16

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042546	cell wall biogenesis
biological_process	GO:0071555	cell wall organization
biological_process	GO:0010411	xyloglucan metabolic process
biological_process	GO:0016998	cell wall macromolecule catabolic process
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0006073	cellular glucan metabolic process
cellular_component	GO:0048046	apoplast
cellular_component	GO:0005618	cell wall
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function	GO:0016762	xyloglucan:xyloglucosyl transferase activity
molecular_function	GO:0033946	xyloglucan-specific endo-beta-1,4-glucanase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G000150.1	Lsi03G000150.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000757	Glycoside hydrolase family 16	PFAM	PF00722	Glyco_hydro_16	coord: 40..227 score: 2.5
IPR000757	Glycoside hydrolase family 16	PROFILE	PS51762	GH16_2	coord: 18..235 score: 29
IPR010713	Xyloglucan endo-transglycosylase, C-terminal	PFAM	PF06955	XET_C	coord: 264..300 score: 8.9
IPR013320	Concanavalin A-like lectin/glucanase domain	GENE3D	G3DSA:2.60.120.200		coord: 33..300 score: 1.2
IPR013320	Concanavalin A-like lectin/glucanase domain	unknown	SSF49899	Concanavalin A-like lectins/glucanases	coord: 38..300 score: 1.47
IPR016455	Xyloglucan endotransglucosylase/hydrolase	PIR	PIRSF005604	EndGlu_transf	coord: 2..300 score: 6.1
None	No IPR available	PANTHER	PTHR31062	FAMILY NOT NAMED	coord: 1..300 score: 7.3E
None	No IPR available	PANTHER	PTHR31062:SF18	SUBFAMILY NOT NAMED	coord: 1..300 score: 7.3E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi03G000150	Csa6G147660	Cucumber (Chinese Long) v2	culsiB449
Lsi03G000150	Csa1G109370	Cucumber (Chinese Long) v2	culsiB044
Lsi03G000150	CSPI06G11840	Wild cucumber (PI 183967)	cpilsiB456
Lsi03G000150	CSPI01G13420	Wild cucumber (PI 183967)	cpilsiB046
Lsi03G000150	Cucsa.292650	Cucumber (Gy14) v1	cgylsiB476
Lsi03G000150	CmaCh13G002420	Cucurbita maxima (Rimu)	cmalsiB212
Lsi03G000150	CmaCh01G005110	Cucurbita maxima (Rimu)	cmalsiB423
Lsi03G000150	CmoCh13G002430	Cucurbita moschata (Rifu)	cmolsiB203
Lsi03G000150	CmoCh01G005430	Cucurbita moschata (Rifu)	cmolsiB410
Lsi03G000150	Cla005097	Watermelon (97103) v1	lsiwmB249
Lsi03G000150	MELO3C026945	Melon (DHL92) v3.5.1	lsimeB243
Lsi03G000150	MELO3C004941	Melon (DHL92) v3.5.1	lsimeB244
Lsi03G000150	ClCG10G020030	Watermelon (Charleston Gray)	lsiwcgB224
Lsi03G000150	ClCG03G002820	Watermelon (Charleston Gray)	lsiwcgB238
Lsi03G000150	MELO3C004941.2	Melon (DHL92) v3.6.1	lsimedB255
Lsi03G000150	Cla97C03G053880	Watermelon (97103) v2	lsiwmbB232
Lsi03G000150	Bhi08G000201	Wax gourd	lsiwgoB337
Lsi03G000150	Cp4.1LG02g12520	Cucurbita pepo (Zucchini)	cpelsiB453
Lsi03G000150	Cp4.1LG20g07210	Cucurbita pepo (Zucchini)	cpelsiB429
Lsi03G000150	CsGy1G013520	Cucumber (Gy14) v2	cgyblsiB041
Lsi03G000150	Carg12287	Silver-seed gourd	carlsiB404
Lsi03G000150	Carg26244	Silver-seed gourd	carlsiB976
Lsi03G000150	CsaV3_1G013610	Cucumber (Chinese Long) v3	cuclsiB048

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi03G000150	Bottle gourd (USVL1VR-Ls)	lsilsiB041
Lsi03G000150	Cucurbita maxima (Rimu)	cmalsiB525
Lsi03G000150	Cucurbita moschata (Rifu)	cmolsiB510
Lsi03G000150	Cucurbita pepo (Zucchini)	cpelsiB242
Lsi03G000150	Watermelon (Charleston Gray)	lsiwcgB237
Lsi03G000150	Watermelon (97103) v1	lsiwmB274
Lsi03G000150	Cucumber (Gy14) v2	cgyblsiB415
Lsi03G000150	Melon (DHL92) v3.6.1	lsimedB254
Lsi03G000150	Silver-seed gourd	carlsiB310
Lsi03G000150	Cucumber (Chinese Long) v3	cuclsiB478
Lsi03G000150	Watermelon (97103) v2	lsiwmbB231