Lsi02G026430 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi02G026430
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionSerine/threonine-protein kinase TIO
Locationchr02 : 32857684 .. 32858991 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAGAAATTCAGTGGAAGAAGCCGAAGCAGAAGAAACCCCATTAATCACTTCTTCTTCTTCTTTCAACAATGGAATCCAATGGGAACGAGGGCGGCTCATTGGAAAAGGAAGCTTTGGTTCGGTTTTCTTGGCTTCTCGCAAACCACACATCTCTAAATACACTATTTTCCCTTCGATAATGGCTGTTAAATCCGCTGAAATTTCCGTTTCCGAAACCCTACAAAAGGAGAAACAAAATTACGACAACTTGAAAGGATGCAATTCATTAATCCAATGCTTCGCCGAAGAGATTACTACTGACCATAACGGTCACATGATCTATAATTTGCTGCTTGAGGTCGCCGCTGGAGGAACCTTAGCTCATCATATCAACAACACAGGTAACTAATCGATTAAATTACTCTACAATTTAACAAAGAATATTATAATCTCAAATCTTATATCTTAATTAATCATCCAATTTAATTTCTTACTTAATTTTCTCTTTGCCCTAAACCTAGGGTTATACTAAACTTTTGCCTCTTCTTTCAAATTTAGTTGGTTAATTATATTAATTTATCTAACCTTAACATTGGTAATTTCATACTAAGTATTATTGATTTCCACTTACTAACATAATAGTGTAAAATTTTGGTGGTTTTCTTCGAAAATTTAATTTATTGGTTGCTCTATCAATGCAGGTGGAAAGGGTTTACCAGAAAATGAAGTTCGGAGTTACACGAAATCAATAATCAAAGGATTAATTCACATTCACAAAAACCAATACGTTCACTGTGATTTGAAGCCTGCGAATATTTTATTGGTGCCGAGGAATACTACAAAGGGCCGCCGATTCGTGGCAAAGATCGCCGATCTAGGGTTGGCGAGGAGAAGCAGCAAGACAAAGCCAAGCTATTGTTTGGGAGGGACATTGTCCTACATGGCGCCTGAGACATTGATTGACAGCGTGCAAGAATCGTCGAGTGATATTTGGGCACTCGGCTGCCTCGTCCTCCAAATGCTCACCGGAAACCTCCCGTGGACTGCCTCGGACAAGGCTGGGATTGTGAAACAGATCACGGAGGATTATGGAATGCCAAAGATTCCAGAAGGGTTGTCAGCGGAGGCAAGTCGGTTTTTGAAGAATTGCTTTGTGAGGAATCCGACGTTCAGGTTCACGGCGGAGATGCTTATGATAGTGCCGTTTGTGGCGGGGGTTGAAGATCAGGAAGAAGATGAAGATTTTGTGAGAGCTCCATCATTTGTGAAGAAGAGGCCTAAACAGTTTAAGAGGCAAAGAATAATTCCAATTAAAGCTGTGTAA

mRNA sequence

ATGAAGAGAAATTCAGTGGAAGAAGCCGAAGCAGAAGAAACCCCATTAATCACTTCTTCTTCTTCTTTCAACAATGGAATCCAATGGGAACGAGGGCGGCTCATTGGAAAAGGAAGCTTTGGTTCGGTTTTCTTGGCTTCTCGCAAACCACACATCTCTAAATACACTATTTTCCCTTCGATAATGGCTGTTAAATCCGCTGAAATTTCCGTTTCCGAAACCCTACAAAAGGAGAAACAAAATTACGACAACTTGAAAGGATGCAATTCATTAATCCAATGCTTCGCCGAAGAGATTACTACTGACCATAACGGTCACATGATCTATAATTTGCTGCTTGAGGTCGCCGCTGGAGGAACCTTAGCTCATCATATCAACAACACAGGTGGAAAGGGTTTACCAGAAAATGAAGTTCGGAGTTACACGAAATCAATAATCAAAGGATTAATTCACATTCACAAAAACCAATACGTTCACTGTGATTTGAAGCCTGCGAATATTTTATTGGTGCCGAGGAATACTACAAAGGGCCGCCGATTCGTGGCAAAGATCGCCGATCTAGGGTTGGCGAGGAGAAGCAGCAAGACAAAGCCAAGCTATTGTTTGGGAGGGACATTGTCCTACATGGCGCCTGAGACATTGATTGACAGCGTGCAAGAATCGTCGAGTGATATTTGGGCACTCGGCTGCCTCGTCCTCCAAATGCTCACCGGAAACCTCCCGTGGACTGCCTCGGACAAGGCTGGGATTGTGAAACAGATCACGGAGGATTATGGAATGCCAAAGATTCCAGAAGGGTTGTCAGCGGAGGCAAGTCGGTTTTTGAAGAATTGCTTTGTGAGGAATCCGACGTTCAGGTTCACGGCGGAGATGCTTATGATAGTGCCGTTTGTGGCGGGGGTTGAAGATCAGGAAGAAGATGAAGATTTTGTGAGAGCTCCATCATTTGTGAAGAAGAGGCCTAAACAGTTTAAGAGGCAAAGAATAATTCCAATTAAAGCTGTGTAA

Coding sequence (CDS)

ATGAAGAGAAATTCAGTGGAAGAAGCCGAAGCAGAAGAAACCCCATTAATCACTTCTTCTTCTTCTTTCAACAATGGAATCCAATGGGAACGAGGGCGGCTCATTGGAAAAGGAAGCTTTGGTTCGGTTTTCTTGGCTTCTCGCAAACCACACATCTCTAAATACACTATTTTCCCTTCGATAATGGCTGTTAAATCCGCTGAAATTTCCGTTTCCGAAACCCTACAAAAGGAGAAACAAAATTACGACAACTTGAAAGGATGCAATTCATTAATCCAATGCTTCGCCGAAGAGATTACTACTGACCATAACGGTCACATGATCTATAATTTGCTGCTTGAGGTCGCCGCTGGAGGAACCTTAGCTCATCATATCAACAACACAGGTGGAAAGGGTTTACCAGAAAATGAAGTTCGGAGTTACACGAAATCAATAATCAAAGGATTAATTCACATTCACAAAAACCAATACGTTCACTGTGATTTGAAGCCTGCGAATATTTTATTGGTGCCGAGGAATACTACAAAGGGCCGCCGATTCGTGGCAAAGATCGCCGATCTAGGGTTGGCGAGGAGAAGCAGCAAGACAAAGCCAAGCTATTGTTTGGGAGGGACATTGTCCTACATGGCGCCTGAGACATTGATTGACAGCGTGCAAGAATCGTCGAGTGATATTTGGGCACTCGGCTGCCTCGTCCTCCAAATGCTCACCGGAAACCTCCCGTGGACTGCCTCGGACAAGGCTGGGATTGTGAAACAGATCACGGAGGATTATGGAATGCCAAAGATTCCAGAAGGGTTGTCAGCGGAGGCAAGTCGGTTTTTGAAGAATTGCTTTGTGAGGAATCCGACGTTCAGGTTCACGGCGGAGATGCTTATGATAGTGCCGTTTGTGGCGGGGGTTGAAGATCAGGAAGAAGATGAAGATTTTGTGAGAGCTCCATCATTTGTGAAGAAGAGGCCTAAACAGTTTAAGAGGCAAAGAATAATTCCAATTAAAGCTGTGTAA

Protein sequence

MKRNSVEEAEAEETPLITSSSSFNNGIQWERGRLIGKGSFGSVFLASRKPHISKYTIFPSIMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRSSKTKPSYCLGGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLTGNLPWTASDKAGIVKQITEDYGMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFVAGVEDQEEDEDFVRAPSFVKKRPKQFKRQRIIPIKAV
Homology
[Close]
BLAST of Lsi02G026430 vs. Swiss-Prot
Match: MKKA_DICDI (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum GN=mkkA PE=1 SV=2)

HSP 1 Score: 137.1 bits (344), Expect = 3.5e-31
Identity = 91/296 (30.74%), Postives = 151/296 (51.01%), Query Frame = 1 

Query: 24  NNGIQWERGRLIGKGSFGSVFLASRKPHISKYTIFPSIMAVKSAEISVSETLQKEKQNYD 83
           N+ I+W++G+++G+G +GSV+L   K           + AVK  EI    +  K K    
Sbjct: 165 NHNIKWQKGQILGRGGYGSVYLGLNKDT-------GELFAVKQLEIVDINSDPKLK---- 224

Query: 84  NLKGCNSLIQCFAEEI----TTDHNGHMIY----------NLLLEVAAGGTLAHHINNTG 143
                 ++I  F++EI    +  H+  + Y          ++ LE   GG+++  +   G
Sbjct: 225 ------NMILSFSKEIEVMRSLRHDNIVRYLGTSLDQSFLSVFLEYIPGGSISSLLGKFG 284

Query: 144 GKGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGL 203
                EN ++ YTK I++GL  +H N  +H D+K ANIL+     TKG   + K++D G 
Sbjct: 285 A--FSENVIKVYTKQILQGLSFLHANSIIHRDIKGANILI----DTKG---IVKLSDFGC 344

Query: 204 ARRSSKTKPSY-CLGGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLTGNLPWT-ASDK 263
           ++  S     +  + GT  +MAPE +  +    SSDIW+LGC++++M T   PW+  ++ 
Sbjct: 345 SKSFSGIVSQFKSMQGTPYWMAPEVIKQTGHGRSSDIWSLGCVIVEMATAQPPWSNITEL 404

Query: 264 AGIVKQITEDYGMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFVAGVED 304
           A ++  I     +P IP  +S EA  FL  CF R+P  R  A  L+  PF+  ++D
Sbjct: 405 AAVMYHIASSNSIPNIPSHMSQEAFDFLNLCFKRDPKERPDANQLLKHPFIMNLDD 434

[Close]
BLAST of Lsi02G026430 vs. Swiss-Prot
Match: M3K2_ARATH (Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana GN=ANP2 PE=2 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 1.0e-30
Identity = 89/305 (29.18%), Postives = 155/305 (50.82%), Query Frame = 1 

Query: 13  ETPLITSSSSFNNGIQWERGRLIGKGSFGSVFLA----SRKPHISKYTIFPSIMAVKSAE 72
           ++P   S+      I+W +G+LIG+G+FG+V++     S +    K  +  S  A K   
Sbjct: 52  QSPPNNSTVQIKPPIRWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLITSNCASKEKT 111

Query: 73  ISVSETLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNT 132
            +  + L++E +   NL   N  I  +   +  D       N+LLE   GG+++  +   
Sbjct: 112 QAHIQELEEEVKLLKNLSHPN--IVRYLGTVREDET----LNILLEFVPGGSISSLLEKF 171

Query: 133 GGKGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLG 192
           G    PE+ VR+YT  ++ GL ++H +  +H D+K ANIL+         +   K+AD G
Sbjct: 172 GA--FPESVVRTYTNQLLLGLEYLHNHAIMHRDIKGANILV-------DNQGCIKLADFG 231

Query: 193 LARRSSKTKP---SYCLGGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLTGNLPWTAS 252
            +++ ++      +  + GT  +MAPE ++ +    S+DIW++GC V++M+TG  PW+  
Sbjct: 232 ASKQVAELATISGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQ 291

Query: 253 DK-AGIVKQITEDYGMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFVAGVEDQ 310
            K    +  I      P IP+ +S++A+ FL  C  + P  R TA  L+  PFV G + +
Sbjct: 292 YKEIAAIFHIGTTKSHPPIPDNISSDANDFLLKCLQQEPNLRPTASELLKHPFVTGKQKE 341

[Close]
BLAST of Lsi02G026430 vs. Swiss-Prot
Match: ANP1_ARATH (Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana GN=ANP1 PE=1 SV=2)

HSP 1 Score: 129.4 bits (324), Expect = 7.3e-29
Identity = 89/291 (30.58%), Postives = 147/291 (50.52%), Query Frame = 1 

Query: 27  IQWERGRLIGKGSFGSVFLA----SRKPHISKYTIFPSIMAVKSAEISVSETLQKEKQNY 86
           I W +G+LIG+G+FG+V++     S +    K  +  +  A K    +  + L++E +  
Sbjct: 67  ISWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQELEEEVKLL 126

Query: 87  DNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRSYT 146
            NL   N  I  +   +  D       N+LLE   GG+++  +   G    PE+ VR+YT
Sbjct: 127 KNLSHPN--IVRYLGTVREDDT----LNILLEFVPGGSISSLLEKFGP--FPESVVRTYT 186

Query: 147 KSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRSSKTKP---S 206
           + ++ GL ++H +  +H D+K ANIL+      KG     K+AD G +++ ++      +
Sbjct: 187 RQLLLGLEYLHNHAIMHRDIKGANILV----DNKG---CIKLADFGASKQVAELATMTGA 246

Query: 207 YCLGGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLTGNLPWTASDK-AGIVKQITEDY 266
             + GT  +MAPE ++ +    S+DIW++GC V++M+TG  PW+   K    +  I    
Sbjct: 247 KSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTK 306

Query: 267 GMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFVAGVEDQEEDED 310
             P IP+ LS++A  FL  C    P  R TA  L+  PFV G   +    D
Sbjct: 307 SHPPIPDTLSSDAKDFLLKCLQEVPNLRPTASELLKHPFVMGKHKESASTD 342

[Close]
BLAST of Lsi02G026430 vs. Swiss-Prot
Match: M3K3_ARATH (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana GN=ANP3 PE=1 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 1.2e-28
Identity = 88/282 (31.21%), Postives = 138/282 (48.94%), Query Frame = 1 

Query: 27  IQWERGRLIGKGSFGSVFLASRKPH-----ISKYTIFPSIMAVKSAEISVSETLQKEKQN 86
           I+W +G LIG G+FG V++           I +  I PS  + +  +  + E L++E Q 
Sbjct: 66  IRWRKGELIGCGAFGRVYMGMNLDSGELLAIKQVLIAPSSASKEKTQGHIRE-LEEEVQL 125

Query: 87  YDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRSY 146
             NL   N +++       +D       N+L+E   GG+++  +   G    PE  +  Y
Sbjct: 126 LKNLSHPN-IVRYLGTVRESDS-----LNILMEFVPGGSISSLLEKFGS--FPEPVIIMY 185

Query: 147 TKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVA--KIADLGLARRSSKTKPS 206
           TK ++ GL ++H N  +H D+K ANIL+  +   +   F A  K+ +L     +   K  
Sbjct: 186 TKQLLLGLEYLHNNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATVNGAKSMK-- 245

Query: 207 YCLGGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLTGNLPWTAS-DKAGIVKQITEDY 266
               GT  +MAPE ++ +    S+DIW++GC V++M TG  PW+    +   V  I    
Sbjct: 246 ----GTPYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTK 305

Query: 267 GMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFVAG 301
             P IPE LS EA  FL  C  + P+ R +A  L+  PFV G
Sbjct: 306 AHPPIPEDLSPEAKDFLMKCLHKEPSLRLSATELLQHPFVTG 332

[Close]
BLAST of Lsi02G026430 vs. Swiss-Prot
Match: YODA_ARATH (Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana GN=YDA PE=1 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 1.2e-28
Identity = 91/290 (31.38%), Postives = 144/290 (49.66%), Query Frame = 1 

Query: 19  SSSSFNNGIQWERGRLIGKGSFGSVFLASRKPHISKYTIFPSIMAVKSAEISVSETLQKE 78
           + ++ + G +W++GRL+G GSFG V+L       S        + + S +    E+ Q+ 
Sbjct: 390 AEATVSPGSRWKKGRLLGMGSFGHVYLGFNSE--SGEMCAMKEVTLCSDDPKSRESAQQL 449

Query: 79  KQNYDNLKGCN--SLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPEN 138
            Q    L      +++Q +  E   D     +Y + LE  +GG++   +   G  G  EN
Sbjct: 450 GQEISVLSRLRHQNIVQYYGSETVDDK----LY-IYLEYVSGGSIYKLLQEYGQFG--EN 509

Query: 139 EVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRSSKT 198
            +R+YT+ I+ GL ++H    VH D+K ANIL+ P     GR    K+AD G+A+  +  
Sbjct: 510 AIRNYTQQILSGLAYLHAKNTVHRDIKGANILVDPH----GR---VKVADFGMAKHITAQ 569

Query: 199 KPSYCLGGTLSYMAPETLIDSVQESSS-DIWALGCLVLQMLTGNLPWTASDKAGIVKQIT 258
                  G+  +MAPE + +S   + + DIW+LGC VL+M T   PW+  +    + +I 
Sbjct: 570 SGPLSFKGSPYWMAPEVIKNSNGSNLAVDIWSLGCTVLEMATTKPPWSQYEGVPAMFKIG 629

Query: 259 EDYGMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFVAGVEDQE 306
               +P IP+ LS E   F++ C  RNP  R TA  L+   FV  V   E
Sbjct: 630 NSKELPDIPDHLSEEGKDFVRKCLQRNPANRPTAAQLLDHAFVRNVMPME 663

[Close]
BLAST of Lsi02G026430 vs. TrEMBL
Match: A0A0A0KBM3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430800 PE=3 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 2.3e-146
Identity = 265/337 (78.64%), Postives = 293/337 (86.94%), Query Frame = 1 

Query: 1   MKRNSVEEAEAEETPLITSSSSFNNGIQWERGRLIGKGSFGSVFLASRKPHISKYTIFPS 60
           MKR S+ E EA +      SS FNNGIQW++GRLIGKGSFGSVFLAS KPH +KY+IFP 
Sbjct: 1   MKRKSMLEKEAAQVE--DRSSEFNNGIQWKQGRLIGKGSFGSVFLASLKPHFTKYSIFPP 60

Query: 61  IMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGT 120
           +MAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCF EEITTDHNGHMIYNLLLEVA GGT
Sbjct: 61  VMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGHMIYNLLLEVATGGT 120

Query: 121 LAHHINNTGGKGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKGRR 180
           LAHHI NTGGKGL EN VR+YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTTK R+
Sbjct: 121 LAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTTKDRQ 180

Query: 181 FVAKIADLGLARRSSKTKPSYCLGGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLTGN 240
           F+AKIADLGLARR+SKTK SYCLGGT SYMAPETLID VQES+SDIWALGC+VL+MLTGN
Sbjct: 181 FIAKIADLGLARRTSKTKASYCLGGTFSYMAPETLIDGVQESASDIWALGCVVLEMLTGN 240

Query: 241 LPWTASDKAGIVKQITEDY-GMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFV 300
             W A+DK GIVK++TE++ GMPKIPEGLS EA+ FLKNC VR P FRFTAEMLM VPFV
Sbjct: 241 RAWAATDKVGIVKEMTENFIGMPKIPEGLSPEATGFLKNCLVRKPEFRFTAEMLMNVPFV 300

Query: 301 AGVEDQEEDEDFVRAPSFVKKRPKQFKRQRIIPIKAV 336
           A  ED E+D   V+AP+FV K P+QFKRQR IPIKAV
Sbjct: 301 AAGEDHEQDSTTVKAPTFVTKWPRQFKRQRTIPIKAV 335

[Close]
BLAST of Lsi02G026430 vs. TrEMBL
Match: A0A0A0K663_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430790 PE=3 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 7.3e-145
Identity = 266/341 (78.01%), Postives = 297/341 (87.10%), Query Frame = 1 

Query: 1   MKRNSVE---EAEAEETPLITSSSSFNNGIQWERGRLIGKGSFGSVFLASRKPHIS-KYT 60
           MKRN      E E E+      SS FNNG+QW+RGRLIGKGSFGSVFLAS KPHI+ KY 
Sbjct: 1   MKRNLTLGGLEVEVED-----GSSEFNNGVQWKRGRLIGKGSFGSVFLASLKPHITIKYC 60

Query: 61  IFPSIMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVA 120
            FPS+MAVKSAEISVSETLQKEKQNYD+LKGCNSLI+CF EEITTDHNGHMIYNLLLEVA
Sbjct: 61  TFPSVMAVKSAEISVSETLQKEKQNYDSLKGCNSLIKCFGEEITTDHNGHMIYNLLLEVA 120

Query: 121 AGGTLAHHINNTGGKGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTT 180
            GGTLAHHI NTGGKGL EN VR+YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTT
Sbjct: 121 TGGTLAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTT 180

Query: 181 KGRRFVAKIADLGLARRSSKTKPSYCLGGTLSYMAPETLIDSVQESSSDIWALGCLVLQM 240
           K R+F+AKIADLGLARR+SKTK SYCLGGT SYMAPET ID VQES+SDIWALGC+VL+M
Sbjct: 181 KDRQFIAKIADLGLARRTSKTKASYCLGGTFSYMAPETFIDGVQESASDIWALGCVVLEM 240

Query: 241 LTGNLPWTASDKAGIVKQITEDY-GMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMI 300
           LTGN  W A++K GI+K++TE++ GMPKIPEGLSAEA+ FLKNCFVR P FRFTAEMLMI
Sbjct: 241 LTGNRAWAATNKVGIMKEMTENFLGMPKIPEGLSAEATMFLKNCFVRKPEFRFTAEMLMI 300

Query: 301 VPFVAGVEDQEEDEDFVRAPSFVKKRPKQFKRQRIIPIKAV 336
           VPFVA VEDQE++ + V+AP+FV K P QFKRQRIIPIKAV
Sbjct: 301 VPFVAAVEDQEQNFNTVKAPTFVTKWPMQFKRQRIIPIKAV 336

[Close]
BLAST of Lsi02G026430 vs. TrEMBL
Match: K4C826_SOLLC (Uncharacterized protein OS=Solanum lycopersicum PE=3 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 2.2e-80
Identity = 158/309 (51.13%), Postives = 210/309 (67.96%), Query Frame = 1 

Query: 13  ETPLITSSSSFNNGIQWERGRLIGKGSFGSVFLASRKPHISKYTIFPSIMAVKSAEISVS 72
           E  ++ + + F +G+ W RG ++GKGSFG V+LA+ K   SK    PS+MAVKSAE+SVS
Sbjct: 8   EVEVVKNVNEFGDGVSWYRGAMLGKGSFGYVYLANLKNPKSKNRYLPSVMAVKSAEVSVS 67

Query: 73  ETLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINN--TGG 132
            ++QKE++   N+KGC  +I+CF +E T  +NG M YNLLLE  +GGTLA  IN    G 
Sbjct: 68  GSIQKEREVLSNIKGCPYVIRCFGDETTNGNNGVMAYNLLLEYGSGGTLAQRINKLGNGN 127

Query: 133 KGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLA 192
           +GL E EVR YT+S+++GL HIH   YVHCD+KP N+LLVP ++     F AKI DLGLA
Sbjct: 128 RGLAEFEVRLYTRSMLRGLNHIHAIGYVHCDMKPENVLLVPSSSKGSVEFRAKIGDLGLA 187

Query: 193 RRSSKTKPSYC---LGGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLTGNLPWTASDK 252
           +R +++K         GT  Y++PE + D+VQES +DIWALGC+VL+MLTG  PW   D 
Sbjct: 188 KRENQSKKRRLEPYWRGTPMYLSPEAVADNVQESPADIWALGCIVLEMLTGKPPWDRKDA 247

Query: 253 AGIVKQITEDYGMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFVA-------G 310
             ++K+I E + +P IP  LS EA  FLK CFVR PT+R+TAEML+I PFV        G
Sbjct: 248 EDVLKKIGEGHELPTIPGDLSKEAKDFLKGCFVRKPTYRWTAEMLLIHPFVEGLSDDDDG 307

[Close]
BLAST of Lsi02G026430 vs. TrEMBL
Match: M1CRS2_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG401028487 PE=3 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 8.3e-80
Identity = 160/314 (50.96%), Postives = 216/314 (68.79%), Query Frame = 1 

Query: 5   SVEEAEAEETPLITSSSSFNNGIQWERGRLIGKGSFGSVFLASRKPHISKYTIFPSIMAV 64
           S ++ E EE   + + + F +G+ W RG ++GKGSFG V+LA+ K   SK    PS+MAV
Sbjct: 3   SKKKIEVEE---LKNVNEFGDGVSWYRGAMLGKGSFGYVYLANLKNPNSKNRYLPSVMAV 62

Query: 65  KSAEISVSETLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHH 124
           KSAE+SVS ++QKE++   N+KGC  +I+CF +E T+ +NG M YNLLLE  +GGTLA  
Sbjct: 63  KSAEVSVSGSIQKEREVLSNIKGCPYVIRCFGDETTSGNNGVMAYNLLLEYGSGGTLAER 122

Query: 125 INNTGGKGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKI 184
           INN   +GL E EVR YT+S+++GL HIH   YVHCD+KP N+LLVP ++     F AKI
Sbjct: 123 INN---RGLAEFEVRLYTRSMLRGLNHIHAIGYVHCDMKPENVLLVPSSSKGSVEFRAKI 182

Query: 185 ADLGLARRSSKTKPSYC---LGGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLTGNLP 244
            DLGLA+R +++K         GT  Y++PE + D+VQES +DIWALGC+VL+MLTG  P
Sbjct: 183 GDLGLAKRENQSKKRRLEPYWRGTPMYLSPEAVADNVQESPADIWALGCIVLEMLTGKPP 242

Query: 245 WTASDKAGIVKQITEDYGMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFVA-- 304
           W   D   ++++I E + +P+IP  LS EA  FLK CFVR PT+R+TAEML+I PFV   
Sbjct: 243 WNRKDAEDVLRKIGEGHELPEIPGDLSKEAKDFLKGCFVRKPTYRWTAEMLLIHPFVEGL 302

Query: 305 ----GVEDQEEDED 310
               GVE++EE ED
Sbjct: 303 SDDDGVEEREEVED 310

[Close]
BLAST of Lsi02G026430 vs. TrEMBL
Match: M1CRS3_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG401028487 PE=3 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 8.3e-80
Identity = 160/314 (50.96%), Postives = 216/314 (68.79%), Query Frame = 1 

Query: 5   SVEEAEAEETPLITSSSSFNNGIQWERGRLIGKGSFGSVFLASRKPHISKYTIFPSIMAV 64
           S ++ E EE   + + + F +G+ W RG ++GKGSFG V+LA+ K   SK    PS+MAV
Sbjct: 3   SKKKIEVEE---LKNVNEFGDGVSWYRGAMLGKGSFGYVYLANLKNPNSKNRYLPSVMAV 62

Query: 65  KSAEISVSETLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHH 124
           KSAE+SVS ++QKE++   N+KGC  +I+CF +E T+ +NG M YNLLLE  +GGTLA  
Sbjct: 63  KSAEVSVSGSIQKEREVLSNIKGCPYVIRCFGDETTSGNNGVMAYNLLLEYGSGGTLAER 122

Query: 125 INNTGGKGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKI 184
           INN   +GL E EVR YT+S+++GL HIH   YVHCD+KP N+LLVP ++     F AKI
Sbjct: 123 INN---RGLAEFEVRLYTRSMLRGLNHIHAIGYVHCDMKPENVLLVPSSSKGSVEFRAKI 182

Query: 185 ADLGLARRSSKTKPSYC---LGGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLTGNLP 244
            DLGLA+R +++K         GT  Y++PE + D+VQES +DIWALGC+VL+MLTG  P
Sbjct: 183 GDLGLAKRENQSKKRRLEPYWRGTPMYLSPEAVADNVQESPADIWALGCIVLEMLTGKPP 242

Query: 245 WTASDKAGIVKQITEDYGMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFVA-- 304
           W   D   ++++I E + +P+IP  LS EA  FLK CFVR PT+R+TAEML+I PFV   
Sbjct: 243 WNRKDAEDVLRKIGEGHELPEIPGDLSKEAKDFLKGCFVRKPTYRWTAEMLLIHPFVEGL 302

Query: 305 ----GVEDQEEDED 310
               GVE++EE ED
Sbjct: 303 SDDDGVEEREEVED 310

[Close]
BLAST of Lsi02G026430 vs. TAIR10
Match: AT4G36950.1 (AT4G36950.1 mitogen-activated protein kinase kinase kinase 21)

HSP 1 Score: 203.8 bits (517), Expect = 1.7e-52
Identity = 121/288 (42.01%), Postives = 171/288 (59.38%), Query Frame = 1 

Query: 27  IQWERGRLIGKGSFGSVFLASRKPHISKYTIFPSIMAVKSAEISVSETLQKEKQNYDNLK 86
           ++W R   IG GSF +V LA+     SK   FPS+MAVKS+ +  S  L+ E+   D+L 
Sbjct: 1   MEWIRRETIGHGSFSTVSLATTSGSSSK--AFPSLMAVKSSGVVCSAALRNERDVLDDLG 60

Query: 87  GCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRSYTKSII 146
            C+ +++CF E  T + NG  IYNL LE A+GG+LA  I ++G + LPE EVR +T+SI+
Sbjct: 61  DCSEIVRCFGEGRTVE-NGEEIYNLFLEYASGGSLADRIKSSG-EALPEFEVRRFTRSIV 120

Query: 147 KGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRSSKTKPSYCLGGTL 206
           KGL HIH N + HCD+K  N+L+      K       I+D GLA+R S  +    + GT 
Sbjct: 121 KGLCHIHGNGFTHCDIKLENVLVFGDGDVK-------ISDFGLAKRRSG-EVCVEIRGTP 180

Query: 207 SYMAPETLIDSVQESSSDIWALGCLVLQMLTGNLPWTASDKA-----GIVKQITEDYGMP 266
            YMAPE++     ES +DIWALGC V++M +G   W   D        ++ +I     +P
Sbjct: 181 LYMAPESVNHGEFESPADIWALGCSVVEMSSGKTAWCLEDGVMNNVMSLLVRIGSGDEVP 240

Query: 267 KIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFVAGVEDQEEDED 310
           +IP  LS E   F+  CFV+N   R+TAEML+  PF+A V+D+  +ED
Sbjct: 241 RIPVELSEEGKDFVSKCFVKNAAERWTAEMLLDHPFLA-VDDESGEED 275

[Close]
BLAST of Lsi02G026430 vs. TAIR10
Match: AT5G67080.1 (AT5G67080.1 mitogen-activated protein kinase kinase kinase 19)

HSP 1 Score: 201.4 bits (511), Expect = 8.5e-52
Identity = 121/295 (41.02%), Postives = 175/295 (59.32%), Query Frame = 1 

Query: 27  IQWERGRLIGKGSFGSVFLASRKPHISKYTIFPSIMAVKSAEISVSETLQKEKQNYDNL- 86
           ++W RG  IG G+F +V LA+R  + S    FP +MAVKSA+   + +L  EK   DNL 
Sbjct: 1   MEWIRGETIGYGTFSTVSLATRSNNDSGE--FPPLMAVKSADSYGAASLANEKSVLDNLG 60

Query: 87  KGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRSYTKSI 146
             CN +++CF E+ T + NG  ++NL LE A+ G+L  ++    G+G+PE+ VR +T S+
Sbjct: 61  DDCNEIVRCFGEDRTVE-NGEEMHNLFLEYASRGSLESYLKKLAGEGVPESTVRRHTGSV 120

Query: 147 IKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARR-SSKTKPSY--CL 206
           ++GL HIH N + HCDLK  NILL             KIAD GLA+R    T  +Y   +
Sbjct: 121 LRGLRHIHANGFAHCDLKLGNILLFGDG-------AVKIADFGLAKRIGDLTALNYGVQI 180

Query: 207 GGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLTGNLPWT---ASDKAGIVKQITEDYG 266
            GT  YMAPE++ D+   S  D+WALGC+V++M +G   W+    S+   ++ +I     
Sbjct: 181 RGTPLYMAPESVNDNEYGSEGDVWALGCVVVEMFSGKTAWSLKEGSNFMSLLLRIGVGDE 240

Query: 267 MPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFVAGVEDQE---EDEDFV 312
           +P IPE LS +   FL  CFV++P  R+TAEML+  PFV    D +   ++EDFV
Sbjct: 241 VPMIPEELSEQGRDFLSKCFVKDPKKRWTAEMLLNHPFVTVDVDHDVLVKEEDFV 285

[Close]
BLAST of Lsi02G026430 vs. TAIR10
Match: AT3G50310.1 (AT3G50310.1 mitogen-activated protein kinase kinase kinase 20)

HSP 1 Score: 199.9 bits (507), Expect = 2.5e-51
Identity = 114/292 (39.04%), Postives = 173/292 (59.25%), Query Frame = 1 

Query: 27  IQWERGRLIGKGSFGSVFLASRKPHISKYTIFPSIMAVKSAEISVSETLQKEKQNYDNLK 86
           ++W RG  IG G+F +V  A++  +      FP+++AVKS +   + +L  EK   D+L 
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGD---FPALIAVKSTDAYGAASLSNEKSVLDSLG 60

Query: 87  GCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRSYTKSII 146
            C  +I+C+ E+ T + NG  ++NLLLE A+ G+LA ++   GG+GLPE+ VR +T S++
Sbjct: 61  DCPEIIRCYGEDSTVE-NGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVL 120

Query: 147 KGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARR----SSKTKPSYCL 206
           +GL HIH   + HCD+K ANILL    +        KIAD GLA R     +  + S  +
Sbjct: 121 RGLRHIHAKGFAHCDIKLANILLFNDGS-------VKIADFGLAMRVDGDLTALRKSVEI 180

Query: 207 GGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLTGNLPWTASDKA---GIVKQITEDYG 266
            GT  YMAPE + D+   S++D+WALGC V++M +G   W+  + +    ++ +I     
Sbjct: 181 RGTPLYMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDE 240

Query: 267 MPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFVAGVEDQEEDEDFV 312
           +PKIPE LS E   FL  CFV++P  R+TAEML+   FV    + +  E+FV
Sbjct: 241 LPKIPEMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFV 281

[Close]
BLAST of Lsi02G026430 vs. TAIR10
Match: AT4G26890.1 (AT4G26890.1 mitogen-activated protein kinase kinase kinase 16)

HSP 1 Score: 172.2 bits (435), Expect = 5.5e-43
Identity = 104/283 (36.75%), Postives = 157/283 (55.48%), Query Frame = 1 

Query: 27  IQWERGRLIGKGSFGSVFLASRKPHISKYTIFPSIMAVKSAEISVSETLQKEKQNYDNLK 86
           I W RG +IG+GS  +V +A              + AVKSA++S S  LQKE+     L 
Sbjct: 3   INWTRGPIIGRGSTATVSIAISSSG--------ELFAVKSADLSSSSLLQKEQSILSTLS 62

Query: 87  GCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRSYTKSII 146
             + +++     +T + NG ++YN+L+E  +GG L   I N+GGK LPE E+RSYT+ I+
Sbjct: 63  SPH-MVKYIGTGLTRESNG-LVYNILMEYVSGGNLHDLIKNSGGK-LPEPEIRSYTRQIL 122

Query: 147 KGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRSSKTKPSYCLGGTL 206
            GL+++H+   VHCDLK  N+L+           V KIAD+G A+   K++      GT 
Sbjct: 123 NGLVYLHERGIVHCDLKSHNVLVEENG-------VLKIADMGCAKSVDKSE----FSGTP 182

Query: 207 SYMAPETLIDSVQESSSDIWALGCLVLQMLTGNLPW-TASDKAGIVKQITEDYGMPKIPE 266
           ++MAPE      Q   +D+WALGC +++M+TG+ PW   +D    + +I      P IP 
Sbjct: 183 AFMAPEVARGEEQRFPADVWALGCTMIEMMTGSSPWPELNDVVAAMYKIGFSGESPAIPA 242

Query: 267 GLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFVAGVEDQEEDE 309
            +S +A  FLKNC   +   R+T E L+  PF   ++D EE +
Sbjct: 243 WISDKAKDFLKNCLKEDQKQRWTVEELLKHPF---LDDDEESQ 260

[Close]
BLAST of Lsi02G026430 vs. TAIR10
Match: AT5G27510.1 (AT5G27510.1 Protein kinase superfamily protein)

HSP 1 Score: 164.9 bits (416), Expect = 8.8e-41
Identity = 95/237 (40.08%), Postives = 134/237 (56.54%), Query Frame = 1 

Query: 63  AVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFAEEITT--DHNGHMIYNLLLEVAAGGT 122
           AVKS++   S  L KE      LKGC  +IQCF  ++    D  G+ +Y LLLE A+ G+
Sbjct: 35  AVKSSDDENS--LLKEFHILSELKGCPRIIQCFGNDLEEGFDDKGNRVYKLLLEYASEGS 94

Query: 123 LAHHINNTGGKGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRF 182
           L+  +NN   + LP+  +R +T+ I++GL+ IH + YVHCDLKP N+L+ P     G  +
Sbjct: 95  LSDFMNNCVDRKLPDLMIRDFTRMILQGLVSIHSHGYVHCDLKPENVLVFP----CGDSY 154

Query: 183 VAKIADLGLARRSSKT----KPSYCLGGTLSYMAPETLIDSVQESSSDIWALGCLVLQML 242
             KI+D GL+ +  +     K  Y   GTL+YM PE+L D V   + D+W+LGCLVL+M 
Sbjct: 155 EVKISDFGLSLQVGEVPDHWKIEYPFVGTLNYMPPESLHDGVANKTLDLWSLGCLVLEMY 214

Query: 243 TGNLPWTASDKAGIVKQITEDYGMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLM 294
               PW        V  I  +   P+IPE L  +A  F++ CF RNP  R TA  L+
Sbjct: 215 VCKKPWIGFIPEDFV-YILSNGNPPEIPESLPCDARAFIQKCFSRNPKERGTASELL 264

[Close]
BLAST of Lsi02G026430 vs. NCBI nr
Match: gi|449436643|ref|XP_004136102.1| (PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis sativus])

HSP 1 Score: 526.6 bits (1355), Expect = 3.3e-146
Identity = 265/337 (78.64%), Postives = 293/337 (86.94%), Query Frame = 1 

Query: 1   MKRNSVEEAEAEETPLITSSSSFNNGIQWERGRLIGKGSFGSVFLASRKPHISKYTIFPS 60
           MKR S+ E EA +      SS FNNGIQW++GRLIGKGSFGSVFLAS KPH +KY+IFP 
Sbjct: 1   MKRKSMLEKEAAQVE--DRSSEFNNGIQWKQGRLIGKGSFGSVFLASLKPHFTKYSIFPP 60

Query: 61  IMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGT 120
           +MAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCF EEITTDHNGHMIYNLLLEVA GGT
Sbjct: 61  VMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGHMIYNLLLEVATGGT 120

Query: 121 LAHHINNTGGKGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKGRR 180
           LAHHI NTGGKGL EN VR+YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTTK R+
Sbjct: 121 LAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTTKDRQ 180

Query: 181 FVAKIADLGLARRSSKTKPSYCLGGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLTGN 240
           F+AKIADLGLARR+SKTK SYCLGGT SYMAPETLID VQES+SDIWALGC+VL+MLTGN
Sbjct: 181 FIAKIADLGLARRTSKTKASYCLGGTFSYMAPETLIDGVQESASDIWALGCVVLEMLTGN 240

Query: 241 LPWTASDKAGIVKQITEDY-GMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFV 300
             W A+DK GIVK++TE++ GMPKIPEGLS EA+ FLKNC VR P FRFTAEMLM VPFV
Sbjct: 241 RAWAATDKVGIVKEMTENFIGMPKIPEGLSPEATGFLKNCLVRKPEFRFTAEMLMNVPFV 300

Query: 301 AGVEDQEEDEDFVRAPSFVKKRPKQFKRQRIIPIKAV 336
           A  ED E+D   V+AP+FV K P+QFKRQR IPIKAV
Sbjct: 301 AAGEDHEQDSTTVKAPTFVTKWPRQFKRQRTIPIKAV 335

[Close]
BLAST of Lsi02G026430 vs. NCBI nr
Match: gi|659122990|ref|XP_008461430.1| (PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo])

HSP 1 Score: 525.0 bits (1351), Expect = 9.5e-146
Identity = 263/337 (78.04%), Postives = 295/337 (87.54%), Query Frame = 1 

Query: 1   MKRNSVEEAEAEETPLITSSSSFNNGIQWERGRLIGKGSFGSVFLASRKPHISKYTIFPS 60
           MKRN + E EA +      S+ FNNGIQW+RGRLIGKGSFGSVFLA  KPH +KY+IFP 
Sbjct: 1   MKRNPMLEKEAAQVE--EGSTEFNNGIQWKRGRLIGKGSFGSVFLACLKPHFTKYSIFPP 60

Query: 61  IMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGT 120
           +MAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCF EEITTDHNG MIYNLLLEVA GGT
Sbjct: 61  VMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGRMIYNLLLEVATGGT 120

Query: 121 LAHHINNTGGKGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKGRR 180
           LAHHI NTGGKGL EN VR+YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTTK R+
Sbjct: 121 LAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTTKDRQ 180

Query: 181 FVAKIADLGLARRSSKTKPSYCLGGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLTGN 240
           F+AKIADLGLARR+SKTK SYCLGGT SYMAPETLID VQES+SDIWALGC+VL+MLTGN
Sbjct: 181 FIAKIADLGLARRTSKTKASYCLGGTFSYMAPETLIDGVQESTSDIWALGCVVLEMLTGN 240

Query: 241 LPWTASDKAGIVKQITEDY-GMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFV 300
             W A+DK GI++++TE++ GMPKIPEGLSAEA+ FLKNC VR P FRFTAEML+ VPFV
Sbjct: 241 RAWAATDKVGILQEMTENFLGMPKIPEGLSAEATGFLKNCLVRKPEFRFTAEMLINVPFV 300

Query: 301 AGVEDQEEDEDFVRAPSFVKKRPKQFKRQRIIPIKAV 336
           A  EDQE+D + V+AP+FV K P+QFKRQRIIPIKAV
Sbjct: 301 AAAEDQEQDFNTVKAPTFVTKWPRQFKRQRIIPIKAV 335

[Close]
BLAST of Lsi02G026430 vs. NCBI nr
Match: gi|659122992|ref|XP_008461431.1| (PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo])

HSP 1 Score: 522.3 bits (1344), Expect = 6.2e-145
Identity = 267/339 (78.76%), Postives = 297/339 (87.61%), Query Frame = 1 

Query: 1   MKRNSV-EEAEAEETPLITSSSSFNNGIQWERGRLIGKGSFGSVFLASRKPHIS-KYTIF 60
           MKRNS+ EE E E   +   SS FNNGIQW+RGRLIGKGSFGSVFLAS KPHIS KY+IF
Sbjct: 1   MKRNSMLEELEVE---VEDGSSEFNNGIQWKRGRLIGKGSFGSVFLASLKPHISIKYSIF 60

Query: 61  PSIMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAG 120
           PS+MAVKSAEISVSETLQKEKQNYDNLKGC+SLIQCF EEITTDHNGHMIYNLLLEVA G
Sbjct: 61  PSVMAVKSAEISVSETLQKEKQNYDNLKGCSSLIQCFGEEITTDHNGHMIYNLLLEVATG 120

Query: 121 GTLAHHINNTGGKGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKG 180
           GTLAHHI NTGGKGL EN VR+YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTT  
Sbjct: 121 GTLAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLIPKNNTTTN 180

Query: 181 RRFVAKIADLGLARRSSKTKPSYCLGGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLT 240
           R+F+AKIADLGLARR+SKTK SYCLGGT SYMAPETLID VQES+SDIWALGC+VL+MLT
Sbjct: 181 RQFIAKIADLGLARRTSKTKGSYCLGGTFSYMAPETLIDGVQESTSDIWALGCVVLEMLT 240

Query: 241 GNLPWTASDKAGIVKQITED-YGMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVP 300
           GN  W A+DK GIVK++TE+ +GMPKIP+GLS EA+ FLKNC VR P FRFTAEMLM VP
Sbjct: 241 GNRAWAATDKVGIVKEMTENLHGMPKIPDGLSVEATGFLKNCLVRKPEFRFTAEMLMNVP 300

Query: 301 FVAGVEDQEEDEDFVRAPSFVKKRPKQFKRQRIIPIKAV 336
           FVA   DQE+D + ++AP+ V K P+QFKRQRIIPIKAV
Sbjct: 301 FVAAAVDQEQDFNTMKAPTSVTKWPRQFKRQRIIPIKAV 336

[Close]
BLAST of Lsi02G026430 vs. NCBI nr
Match: gi|449436645|ref|XP_004136103.1| (PREDICTED: protein kinase byr2-like [Cucumis sativus])

HSP 1 Score: 521.5 bits (1342), Expect = 1.1e-144
Identity = 266/341 (78.01%), Postives = 297/341 (87.10%), Query Frame = 1 

Query: 1   MKRNSVE---EAEAEETPLITSSSSFNNGIQWERGRLIGKGSFGSVFLASRKPHIS-KYT 60
           MKRN      E E E+      SS FNNG+QW+RGRLIGKGSFGSVFLAS KPHI+ KY 
Sbjct: 1   MKRNLTLGGLEVEVED-----GSSEFNNGVQWKRGRLIGKGSFGSVFLASLKPHITIKYC 60

Query: 61  IFPSIMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVA 120
            FPS+MAVKSAEISVSETLQKEKQNYD+LKGCNSLI+CF EEITTDHNGHMIYNLLLEVA
Sbjct: 61  TFPSVMAVKSAEISVSETLQKEKQNYDSLKGCNSLIKCFGEEITTDHNGHMIYNLLLEVA 120

Query: 121 AGGTLAHHINNTGGKGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTT 180
            GGTLAHHI NTGGKGL EN VR+YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTT
Sbjct: 121 TGGTLAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTT 180

Query: 181 KGRRFVAKIADLGLARRSSKTKPSYCLGGTLSYMAPETLIDSVQESSSDIWALGCLVLQM 240
           K R+F+AKIADLGLARR+SKTK SYCLGGT SYMAPET ID VQES+SDIWALGC+VL+M
Sbjct: 181 KDRQFIAKIADLGLARRTSKTKASYCLGGTFSYMAPETFIDGVQESASDIWALGCVVLEM 240

Query: 241 LTGNLPWTASDKAGIVKQITEDY-GMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMI 300
           LTGN  W A++K GI+K++TE++ GMPKIPEGLSAEA+ FLKNCFVR P FRFTAEMLMI
Sbjct: 241 LTGNRAWAATNKVGIMKEMTENFLGMPKIPEGLSAEATMFLKNCFVRKPEFRFTAEMLMI 300

Query: 301 VPFVAGVEDQEEDEDFVRAPSFVKKRPKQFKRQRIIPIKAV 336
           VPFVA VEDQE++ + V+AP+FV K P QFKRQRIIPIKAV
Sbjct: 301 VPFVAAVEDQEQNFNTVKAPTFVTKWPMQFKRQRIIPIKAV 336

[Close]
BLAST of Lsi02G026430 vs. NCBI nr
Match: gi|460392140|ref|XP_004241675.1| (PREDICTED: mitogen-activated protein kinase kinase kinase NPK1-like [Solanum lycopersicum])

HSP 1 Score: 307.4 bits (786), Expect = 3.1e-80
Identity = 158/309 (51.13%), Postives = 210/309 (67.96%), Query Frame = 1 

Query: 13  ETPLITSSSSFNNGIQWERGRLIGKGSFGSVFLASRKPHISKYTIFPSIMAVKSAEISVS 72
           E  ++ + + F +G+ W RG ++GKGSFG V+LA+ K   SK    PS+MAVKSAE+SVS
Sbjct: 8   EVEVVKNVNEFGDGVSWYRGAMLGKGSFGYVYLANLKNPKSKNRYLPSVMAVKSAEVSVS 67

Query: 73  ETLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINN--TGG 132
            ++QKE++   N+KGC  +I+CF +E T  +NG M YNLLLE  +GGTLA  IN    G 
Sbjct: 68  GSIQKEREVLSNIKGCPYVIRCFGDETTNGNNGVMAYNLLLEYGSGGTLAQRINKLGNGN 127

Query: 133 KGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLA 192
           +GL E EVR YT+S+++GL HIH   YVHCD+KP N+LLVP ++     F AKI DLGLA
Sbjct: 128 RGLAEFEVRLYTRSMLRGLNHIHAIGYVHCDMKPENVLLVPSSSKGSVEFRAKIGDLGLA 187

Query: 193 RRSSKTKPSYC---LGGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLTGNLPWTASDK 252
           +R +++K         GT  Y++PE + D+VQES +DIWALGC+VL+MLTG  PW   D 
Sbjct: 188 KRENQSKKRRLEPYWRGTPMYLSPEAVADNVQESPADIWALGCIVLEMLTGKPPWDRKDA 247

Query: 253 AGIVKQITEDYGMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFVA-------G 310
             ++K+I E + +P IP  LS EA  FLK CFVR PT+R+TAEML+I PFV        G
Sbjct: 248 EDVLKKIGEGHELPTIPGDLSKEAKDFLKGCFVRKPTYRWTAEMLLIHPFVEGLSDDDDG 307

The following BLAST results are available for this feature:
BLAST of Lsi02G026430 vs. Swiss-Prot
Match NameE-valueIdentityDescription
MKKA_DICDI3.5e-3130.74Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum GN=... [more]
M3K2_ARATH1.0e-3029.18Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana GN=ANP2... [more]
ANP1_ARATH7.3e-2930.58Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana GN=A... [more]
M3K3_ARATH1.2e-2831.21Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana GN=ANP3... [more]
YODA_ARATH1.2e-2831.38Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana GN=Y... [more]
BLAST of Lsi02G026430 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KBM3_CUCSA2.3e-14678.64Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430800 PE=3 SV=1[more]
A0A0A0K663_CUCSA7.3e-14578.01Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430790 PE=3 SV=1[more]
K4C826_SOLLC2.2e-8051.13Uncharacterized protein OS=Solanum lycopersicum PE=3 SV=1[more]
M1CRS2_SOLTU8.3e-8050.96Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG401028487 PE=3 SV=1[more]
M1CRS3_SOLTU8.3e-8050.96Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG401028487 PE=3 SV=1[more]
BLAST of Lsi02G026430 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G36950.11.7e-5242.01 mitogen-activated protein kinase kinase kinase 21[more]
AT5G67080.18.5e-5241.02 mitogen-activated protein kinase kinase kinase 19[more]
AT3G50310.12.5e-5139.04 mitogen-activated protein kinase kinase kinase 20[more]
AT4G26890.15.5e-4336.75 mitogen-activated protein kinase kinase kinase 16[more]
AT5G27510.18.8e-4140.08 Protein kinase superfamily protein[more]
BLAST of Lsi02G026430 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449436643|ref|XP_004136102.1|3.3e-14678.64PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis sativu... [more]
gi|659122990|ref|XP_008461430.1|9.5e-14678.04PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo][more]
gi|659122992|ref|XP_008461431.1|6.2e-14578.76PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo][more]
gi|449436645|ref|XP_004136103.1|1.1e-14478.01PREDICTED: protein kinase byr2-like [Cucumis sativus][more]
gi|460392140|ref|XP_004241675.1|3.1e-8051.13PREDICTED: mitogen-activated protein kinase kinase kinase NPK1-like [Solanum lyc... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0006468protein phosphorylation
Vocabulary: Molecular Function
TermDefinition
GO:0005524ATP binding
GO:0004672protein kinase activity
Vocabulary: INTERPRO
TermDefinition
IPR017441Protein_kinase_ATP_BS
IPR011009Kinase-like_dom_sf
IPR008271Ser/Thr_kinase_AS
IPR000719Prot_kinase_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0009069 serine family amino acid metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G026430.1Lsi02G026430.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 30..297
score: 2.7
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 29..298
score: 6.1
IPR000719Protein kinase domainPROFILEPS50011PROTEIN_KINASE_DOMcoord: 29..298
score: 36
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 157..169
scor
IPR011009Protein kinase-like domainunknownSSF56112Protein kinase-like (PK-like)coord: 28..305
score: 3.93
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 35..65
scor
NoneNo IPR availableGENE3DG3DSA:1.10.510.10coord: 146..302
score: 4.5
NoneNo IPR availableGENE3DG3DSA:3.30.200.20coord: 28..145
score: 2.5
NoneNo IPR availablePANTHERPTHR24361MITOGEN-ACTIVATED KINASE KINASE KINASEcoord: 16..309
score: 6.0
NoneNo IPR availablePANTHERPTHR24361:SF303SUBFAMILY NOT NAMEDcoord: 16..309
score: 6.0