Lsi02G024310 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi02G024310
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Pectin lyase-like superfamily protein, putative
Location	chr02 : 30856768 .. 30859961 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTAAAACAAAGAGCTCATATTCATAACAAAGCACAATCTGGGGGGTTAGAAGTCACCGTCACCCATGGCCATTCCAGAGAGATTTCACATCGCCGCCCTCCCTGCCACCGCCGTCCGTTGGTTAGTATTAGGCTTGGCATGTCTCTCTTCTATGGCCCAGTGCAGCTCCGCCGTTGCACCCAATTACTCCGGTGACTTTTCTGGCATTCGAGGCCGCCTCCTCGTCGGAGCAGCGCCCGCCACCACCACCCCCACTCCCACTACCACCGCCGCCGCCGCCGCAGCAAATGTTTTCGATATTACAACCCATGGTGCCAAGCCTGATGGCACGACAGATAATACACAGGTGAGTTGGGCCAAGACAGGTGTCATATATAAACCCTCCAACCTATTGACGCTTCTGCAATTATCTTTTTTTCATTTTCTTTATTTGTCGATTTCATAACAATGAACATGGATGTTAAACATATTTGAGATTTGGAAATATGCAGGCGTTCATGCAGACGTGGGTTGCAGCATGTCACTCAGGTGGCCCGGCGAAGGTGGTATTCCCTGCGGGAACATTTTTGACAGGGCCGTTGGTATACGCTGGGCCGTGCGACGGGCCGATGACGGTTGAAATTCAAGGGACTGTGAAGGCAACAACTGATATTACTGAATATTCATCTGCAGAGTGGATTCTATTTGAATCTATCACTGGGTTGAGTCTCATTGGTAGTGGAACCTTTGATGGACAAGGTGCAGAAACATGGATGTATAATGATTGTAGCAAACACACTCGTTGCATTCTTCCCCCAACTGTAAGCATTCATAATTCTATTTCAATTTCTTCTTTCAATTTTGTGTTTAGCGTGTCTTAATTTTATGCATTATCCATTAATTTAAGATGTTATGAAAGTTAGTGACCTATTGGACACTAACTCCAAAGTTTAAAGACTAAAATTAGGATAGACAGGTTACTCTTCTTGAATCCCATGTAAAGTCTAAATGGGCATTCGACCCAAAAAAAAAATCGAGATCCCAGGAATGGTTAACTCGGGCATTTGATGATCCTACAAAATTAAAAATCAAGAACCTAATTACAATCTTTATAAAATATAGACGGGCTACTCCATATTATCTAATACTATTTGATAACTATTACTATGTGCCACCATGTTTTCAAAAGTGAAGCAAAGATTTGAATGCTAAAAAAATAATTTTTGTTTCTTTTTCAAAACATTGCTTTTATTTTATAACTTAACTATGAATTCAAATATTTCGAAGTACAGTTTTGGGTTGTCGGGATTCAAATACATTTGTTTGCCTATAAGCACAACATTTATTTTGATGGATCAAGTAAACGAATTAAAAATGTACAGAAGGCAGGTTTTTGTTAATTATTTTAGGATTTTGTGTAATGAATTTGGATGCAGTCAATAAAGTTCAACAAGGTGACCAATGGATTGATGGAGGGGATTTCTTCATTGAACAGCAAAGCCTTCCACATATTCTTGGTTTTAAGCCACAATATAAAAGTCAACAATCTCCGCATAACAGCTCCAGGTAACAGCCCCAACACGGATGGTATTCACATAAGCCAAACTGATGTTGTTAATATTACAAATAGCATCATTGGTACTGGAGATGATTGTGTTTCAATTGGCCATGGTTCCACCAATATCACTGTTTTCAACGTCACTTGTGGCCCAGGACATGGCATCAGGTATATATACATATTGTTGAGAATTCTACATTAGAAAGATCGAGAAAGCTGACAATTTTTACACTCACACAATGTACATGTTTAACTAATACACACATACATACTCACACAATACATTCATTCATTAGAAATATGAGTTCTAAGGGTCTGAGAGGCTATTTGGAATAACTTTTAAGCACTTAGTCCAACACTGTCCTAAATTTAGTTTACAAGTTTGTGATTTATAAAATTAGGGTTACTAGGATTGAGAGGTTTAATTGCATGTAGTTTATGTTTTATAGGTGGAGAGATCGAACAAATTCATAATTTGAATATGGTTTAGCTCGGTTTCAATTTAATATTTGTATAGTTTTAGAAGTTCTAATTTGGTCGTGTTTTCTTTTGGGACACATGTGATAAGATAGCATATTTAAAGAAAGTGATGAATAGTATTAGATAAACATGAAGTTGTATTAAAACCATTTGACATTCAAAACTAATTGATAACGAGAGTAGTAATTAATTTATCTTATAAAGATCGATTGTGAAGTTTCTCCATCTTTTCAATGCTGGATCCTTAACATGTCACCGAACCTTTGTAACTTGATTTTGTTGCAACCATCTTTTGTAAATTTAATATATCGTTTTCTTAGCCATCCTCTAATAGGATGGTTTGAGAGATTGAAACCAAATCCATCCTAAATTTTTGTATGTATGTAAATTTTGCAGTGTGGGAAGTCTTGGAAAATATAATGATGAAAAGGAGGTGAATGGAATTTTTGTGTCAAATTGCACAATAAAAAACACCACAAATGGAGTAAGGATCAAGACATGGGCTGCTTCACCTCCTGGCCAAGCTACCAGAATCACTTTCCAAGACATTGTCTTAGATTCAGTCCGAAACCCTATCATCATCGATCAAACTTACGGCTCGAAAAAGAAGAAAGCAGTAAGTAAACTAATTAATTAAGAATTCGTTCGATAACAATAAACACAATTACAATTGATCATTGTGTCATGTTTGTCCATGCAGCCATCTCAAGTGAAGGTCAGCAATGTCGAGTTCAAGAACATTCGGGGCACCACGGTGTCCGATGTCGCCGTTTCATTGCAATGCAGCTCTGCCGTGCCGTGCGACGGCATCAAATTGGAAGAGATTGATTTGGCCTTTAATGGCTCTCGTTTAAAGCATCCATTTGCAAATTCATGTTTGAATGCCAAGATTACGGCTATAGGAAAACAGAACCCACCAGCTTGTGTTTAGCTTTGTAACATATATATGTATAGCTCAATTTTTAATTGCATTTTATAATTGGTTCATTCAATTTCGCAAATGTACGAACCATCACAAGTATTACAATAGTGAGGGCTTCGTTTCCACAAAGAACTATCAGTTAATAAACCAATTAAAACTAAATTTATTTGATCAGTTGAATGATCATATTTTTTAAAAAAAAAATTAATTAAATTAAATTATGAAATGCTTAGGACTATAACTTTGCGCCCGTT

mRNA sequence

GTTAAAACAAAGAGCTCATATTCATAACAAAGCACAATCTGGGGGGTTAGAAGTCACCGTCACCCATGGCCATTCCAGAGAGATTTCACATCGCCGCCCTCCCTGCCACCGCCGTCCGTTGGTTAGTATTAGGCTTGGCATGTCTCTCTTCTATGGCCCAGTGCAGCTCCGCCGTTGCACCCAATTACTCCGGTGACTTTTCTGGCATTCGAGGCCGCCTCCTCGTCGGAGCAGCGCCCGCCACCACCACCCCCACTCCCACTACCACCGCCGCCGCCGCCGCAGCAAATGTTTTCGATATTACAACCCATGGTGCCAAGCCTGATGGCACGACAGATAATACACAGGCGTTCATGCAGACGTGGGTTGCAGCATGTCACTCAGGTGGCCCGGCGAAGGTGGTATTCCCTGCGGGAACATTTTTGACAGGGCCGTTGGTATACGCTGGGCCGTGCGACGGGCCGATGACGGTTGAAATTCAAGGGACTGTGAAGGCAACAACTGATATTACTGAATATTCATCTGCAGAGTGGATTCTATTTGAATCTATCACTGGGTTGAGTCTCATTGGTAGTGGAACCTTTGATGGACAAGTCAATAAAGTTCAACAAGGTGACCAATGGATTGATGGAGGGGATTTCTTCATTGAACAGCAAAGCCTTCCACATATTCTTGGTTTTAAGCCACAATATAAAAGTCAACAATCTCCGCATAACAGCTCCAGTGTGGGAAGTCTTGGAAAATATAATGATGAAAAGGAGGTGAATGGAATTTTTGTGTCAAATTGCACAATAAAAAACACCACAAATGGAGTAAGGATCAAGACATGGGCTGCTTCACCTCCTGGCCAAGCTACCAGAATCACTTTCCAAGACATTGTCTTAGATTCAGTCCGAAACCCTATCATCATCGATCAAACTTACGGCTCGAAAAAGAAGAAAGCACCATCTCAAGTGAAGGTCAGCAATGTCGAGTTCAAGAACATTCGGGGCACCACGGTGTCCGATGTCGCCGTTTCATTGCAATGCAGCTCTGCCGTGCCGTGCGACGGCATCAAATTGGAAGAGATTGATTTGGCCTTTAATGGCTCTCGTTTAAAGCATCCATTTGCAAATTCATGTTTGAATGCCAAGATTACGGCTATAGGAAAACAGAACCCACCAGCTTGTGTTTAGCTTTGTAACATATATATGTATAGCTCAATTTTTAATTGCATTTTATAATTGGTTCATTCAATTTCGCAAATGTACGAACCATCACAAGTATTACAATAGTGAGGGCTTCGTTTCCACAAAGAACTATCAGTTAATAAACCAATTAAAACTAAATTTATTTGATCAGTTGAATGATCATATTTTTTAAAAAAAAAATTAATTAAATTAAATTATGAAATGCTTAGGACTATAACTTTGCGCCCGTT

Coding sequence (CDS)

ATGGCCATTCCAGAGAGATTTCACATCGCCGCCCTCCCTGCCACCGCCGTCCGTTGGTTAGTATTAGGCTTGGCATGTCTCTCTTCTATGGCCCAGTGCAGCTCCGCCGTTGCACCCAATTACTCCGGTGACTTTTCTGGCATTCGAGGCCGCCTCCTCGTCGGAGCAGCGCCCGCCACCACCACCCCCACTCCCACTACCACCGCCGCCGCCGCCGCAGCAAATGTTTTCGATATTACAACCCATGGTGCCAAGCCTGATGGCACGACAGATAATACACAGGCGTTCATGCAGACGTGGGTTGCAGCATGTCACTCAGGTGGCCCGGCGAAGGTGGTATTCCCTGCGGGAACATTTTTGACAGGGCCGTTGGTATACGCTGGGCCGTGCGACGGGCCGATGACGGTTGAAATTCAAGGGACTGTGAAGGCAACAACTGATATTACTGAATATTCATCTGCAGAGTGGATTCTATTTGAATCTATCACTGGGTTGAGTCTCATTGGTAGTGGAACCTTTGATGGACAAGTCAATAAAGTTCAACAAGGTGACCAATGGATTGATGGAGGGGATTTCTTCATTGAACAGCAAAGCCTTCCACATATTCTTGGTTTTAAGCCACAATATAAAAGTCAACAATCTCCGCATAACAGCTCCAGTGTGGGAAGTCTTGGAAAATATAATGATGAAAAGGAGGTGAATGGAATTTTTGTGTCAAATTGCACAATAAAAAACACCACAAATGGAGTAAGGATCAAGACATGGGCTGCTTCACCTCCTGGCCAAGCTACCAGAATCACTTTCCAAGACATTGTCTTAGATTCAGTCCGAAACCCTATCATCATCGATCAAACTTACGGCTCGAAAAAGAAGAAAGCACCATCTCAAGTGAAGGTCAGCAATGTCGAGTTCAAGAACATTCGGGGCACCACGGTGTCCGATGTCGCCGTTTCATTGCAATGCAGCTCTGCCGTGCCGTGCGACGGCATCAAATTGGAAGAGATTGATTTGGCCTTTAATGGCTCTCGTTTAAAGCATCCATTTGCAAATTCATGTTTGAATGCCAAGATTACGGCTATAGGAAAACAGAACCCACCAGCTTGTGTTTAG

Protein sequence

MAIPERFHIAALPATAVRWLVLGLACLSSMAQCSSAVAPNYSGDFSGIRGRLLVGAAPATTTPTPTTTAAAAAANVFDITTHGAKPDGTTDNTQAFMQTWVAACHSGGPAKVVFPAGTFLTGPLVYAGPCDGPMTVEIQGTVKATTDITEYSSAEWILFESITGLSLIGSGTFDGQVNKVQQGDQWIDGGDFFIEQQSLPHILGFKPQYKSQQSPHNSSSVGSLGKYNDEKEVNGIFVSNCTIKNTTNGVRIKTWAASPPGQATRITFQDIVLDSVRNPIIIDQTYGSKKKKAPSQVKVSNVEFKNIRGTTVSDVAVSLQCSSAVPCDGIKLEEIDLAFNGSRLKHPFANSCLNAKITAIGKQNPPACV

Homology

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BLAST of Lsi02G024310 vs. Swiss-Prot
Match: PGLR2_PLAAC (Exopolygalacturonase (Fragment) OS=Platanus acerifolia GN=plaa2 PE=1 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 1.6e-37
Identity = 76/154 (49.35%), Postives = 106/154 (68.83%), Query Frame = 1


Query: 215 PHNSSSVGSLGKYNDEKEVNGIFVSNCTIKNTTNGVRIKTWAASPPGQATRITFQDIVLD 274
           P +  S+GSLG+YN+EKEV GI V  CT   T NGVR+KTW  SPPG AT +TFQD+ ++
Sbjct: 221 PGHGISIGSLGRYNNEKEVRGITVKGCTFSGTMNGVRVKTWPNSPPGAATDLTFQDLTMN 280

Query: 275 SVRNPIIIDQ---TYGSKKKKAPSQVKVSNVEFKNIRGTTVSDVAVSLQCSSAVPCDGIK 334
           +V+NP+I+DQ    YG   ++APS++K+SN+ F NIRGT+   VAV + CS  +PC  +K
Sbjct: 281 NVQNPVILDQEYCPYGQCSRQAPSRIKLSNINFNNIRGTSTGKVAVVIACSHGMPCSNMK 340

Query: 335 LEEIDLAFNGSRLKHPFANSCLNAKITAIGKQNP 366
           + EI+L++ G+    P  ++C N K T  GKQ P
Sbjct: 341 IGEINLSYRGA--GGPATSTCSNVKPTFSGKQVP 372

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BLAST of Lsi02G024310 vs. Swiss-Prot
Match: PGLR_TOBAC (Polygalacturonase OS=Nicotiana tabacum GN=PG1 PE=2 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.5e-35
Identity = 79/157 (50.32%), Postives = 103/157 (65.61%), Query Frame = 1


Query: 215 PHNSSSVGSLGKYNDEKEVNGIFVSNCTIKNTTNGVRIKTWAASPPGQATRITFQDIVLD 274
           P +  SVGSLG   DEK V G+FV NCT  NT NGVRIKTW AS PG    + F+DI++ 
Sbjct: 234 PGHGISVGSLGGNPDEKPVVGVFVRNCTFTNTDNGVRIKTWPASHPGVVNDVHFEDIIVQ 293

Query: 275 SVRNPIIIDQTY---GSKKKKAPSQVKVSNVEFKNIRGTTVSDVAVSLQCSSAVPCDGIK 334
           +V NP++IDQ Y       K  PSQVK+S V F+NI+GT+ +  AVSL  S  VPC+GI+
Sbjct: 294 NVSNPVVIDQVYCPFNKCNKDLPSQVKISKVSFQNIKGTSRTQDAVSLLRSKGVPCEGIE 353

Query: 335 LEEIDLAFNGSRLKHPFANSCLNAKITAIGKQNPPAC 369
           + +ID+ ++G   + P  +SC N K +  GKQNPP C
Sbjct: 354 VGDIDITYSGK--EGPAKSSCENIKPSLKGKQNPPVC 388

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BLAST of Lsi02G024310 vs. Swiss-Prot
Match: PGLR2_ARATH (Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana GN=At3g07850 PE=2 SV=2)

HSP 1 Score: 146.0 bits (367), Expect = 8.3e-34
Identity = 87/208 (41.83%), Postives = 119/208 (57.21%), Query Frame = 1


Query: 164 GLSLIGSGTFDGQVNKVQQGDQWIDGGDFFIEQQSLPHILGFKPQYKSQQSPHNSSSVGS 223
           G++LIG+        K++ GD  +  GD   E   + ++         +  P +  S+GS
Sbjct: 249 GVNLIGA--------KIKTGDDCVSIGDG-TENLIVENV---------ECGPGHGISIGS 308

Query: 224 LGKYNDEKEVNGIFVSNCTIKNTTNGVRIKTWAASPPGQATRITFQDIVLDSVRNPIIID 283
           LG+Y +E+ V G+ V  C IKNT NGVRIKTW  SPPG A+ I F+DI +D+V  P++ID
Sbjct: 309 LGRYPNEQPVKGVTVRKCLIKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLID 368

Query: 284 Q---TYGSKKKKAPSQVKVSNVEFKNIRGTTVSDVAVSLQCSSAVPCDGIKLEEIDLAFN 343
           Q    YG  K   PSQVK+S+V  K I+GT+ + VAV L CS  VPC  I L +I+L  N
Sbjct: 369 QEYCPYGHCKAGVPSQVKLSDVTIKGIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHN 428

Query: 344 GSRLKHPFANSCLNAKITAIGKQNPPAC 369
           G   + P  ++C N K    GK  P AC
Sbjct: 429 GK--EGPAVSACSNIKPILSGKLVPAAC 436

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BLAST of Lsi02G024310 vs. Swiss-Prot
Match: PGLR_OENOR (Exopolygalacturonase (Fragment) OS=Oenothera organensis PE=2 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 1.4e-33
Identity = 75/157 (47.77%), Postives = 101/157 (64.33%), Query Frame = 1


Query: 215 PHNSSSVGSLGKYNDEKEVNGIFVSNCTIKNTTNGVRIKTWAASPPGQATRITFQDIVLD 274
           P +  SVGSLG+Y +E+ V GI+V NCTI  + NGVRIKTW  S PG+A+ + FQDI ++
Sbjct: 200 PGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVRIKTWPKSEPGEASEMHFQDITMN 259

Query: 275 SVRNPIIIDQ---TYGSKKKKAPSQVKVSNVEFKNIRGTTVSDVAVSLQCSSAVPCDGIK 334
           SV  PI+IDQ    Y     + PS VK+S + FKNI+GT+ +  AV L CS + PC+G++
Sbjct: 260 SVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFKNIKGTSTTKEAVKLVCSKSFPCNGVE 319

Query: 335 LEEIDLAFNGSRLKHPFANSCLNAKITAIGKQNPPAC 369
           L +IDL ++G     P  + C N K T  GKQ P  C
Sbjct: 320 LADIDLTYSGK--GGPATSVCENIKPTIKGKQIPAIC 354

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BLAST of Lsi02G024310 vs. Swiss-Prot
Match: PGLR_GOSBA (Polygalacturonase OS=Gossypium barbadense GN=G9 PE=2 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 9.2e-33
Identity = 72/157 (45.86%), Postives = 98/157 (62.42%), Query Frame = 1


Query: 215 PHNSSSVGSLGKYNDEKEVNGIFVSNCTIKNTTNGVRIKTWAASPPGQATRITFQDIVLD 274
           P +  S+GSLGK+ +E+ V GI +SNCTI NT+NG RIKTW     G  + I F+DI ++
Sbjct: 242 PGHGISIGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHGGAVSEIHFEDITMN 301

Query: 275 SVRNPIIIDQTY---GSKKKKAPSQVKVSNVEFKNIRGTTVSDVAVSLQCSSAVPCDGIK 334
           +V +PI+IDQ Y      KK   S+VK+SN+ FKNIRGT+    A+   CS + PC  ++
Sbjct: 302 NVSSPILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICSGSSPCQNVE 361

Query: 335 LEEIDLAFNGSRLKHPFANSCLNAKITAIGKQNPPAC 369
           L +ID+  NG+    P  + CLN K   IGK NP  C
Sbjct: 362 LADIDIQHNGA---EPATSQCLNVKPITIGKLNPIPC 395

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BLAST of Lsi02G024310 vs. TrEMBL
Match: A0A067KRA0_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_10580 PE=3 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 3.7e-73
Identity = 168/354 (47.46%), Postives = 215/354 (60.73%), Query Frame = 1


Query: 16  AVRWLVLGL-ACLSSMAQCSSAVAPNYSGDFSGIRGRLLVGAAPATTTPTPTTTAAAAAA 75
           A+ +LVL L +CL++ A   + V         G+R R  +G              + AA 
Sbjct: 11  AITFLVLALFSCLANGALSHTIV---------GLRNRRALGET------------SGAAI 70

Query: 76  NVFDITTHGAKPDGTTDNTQAFMQTWVAACHSGGPAKVVFPAGTFLTGPLVYAGPCDGPM 135
            VFD+T HGAK D  T+N  +F+QTW AAC S  PAKVV P GTFLTGP ++ GPC GP+
Sbjct: 71  TVFDVTEHGAKADDKTNNAMSFIQTWKAACASTAPAKVVIPDGTFLTGPTIFQGPCAGPV 130

Query: 136 TVEIQGTVKATTDITEYSSAEWILFESITGLSLIGSGTFDGQVNKVQQGDQWIDGGDFFI 195
             E+QG VKA+TD++EY+S EW+ FESI GL+  G GTF GQ   V + +      D   
Sbjct: 131 IFEVQGCVKASTDLSEYTSPEWMTFESIDGLTFTGKGTFSGQGPVVWEYNDCKSNEDC-- 190

Query: 196 EQQSLPHILGFKPQYKSQQSPHNSSSVGSLGKYNDEKEVNGIFVSNCTIKNTTNGVRIKT 255
             Q LP                 S SVGSLGKY DE +V+GI V NCT  NTTNG RIKT
Sbjct: 191 --QKLP----------------ASLSVGSLGKYPDEDDVSGILVINCTFSNTTNGARIKT 250

Query: 256 WAASPPGQATRITFQDIVLDSVRNPIIIDQTYGSKKKKAP---SQVKVSNVEFKNIRGTT 315
           ++ S P  A  I +QDI+++ V+NPIIIDQ YG K K+A    S+VKVS+V FKNI+GT+
Sbjct: 251 YSDSGPSNAFGIVYQDIIMNDVKNPIIIDQKYGIKDKEAQPSNSKVKVSDVHFKNIKGTS 310

Query: 316 VSDVAVSLQCSSAVPCDGIKLEEIDLAFNGSRLKHPFANSCLNAKITAIGKQNP 366
           +S  AV LQCSSA PC+G++L +IDL + G+      A  C NAK+T  G QNP
Sbjct: 311 ISAAAVDLQCSSAFPCEGVELSDIDLTYIGNEPSTESA-LCSNAKVTCDGIQNP 322

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BLAST of Lsi02G024310 vs. TrEMBL
Match: A0A0A0K9C4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G404800 PE=3 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.6e-68
Identity = 130/155 (83.87%), Postives = 143/155 (92.26%), Query Frame = 1


Query: 215 PHNSSSVGSLGKYNDEKEVNGIFVSNCTIKNTTNGVRIKTWAASPPGQATRITFQDIVLD 274
           P +  SVGSLGKY DEKEV GIFVSNCTI+NTTNGVRIKTWAASPPGQATRITFQ+IVLD
Sbjct: 279 PGHGISVGSLGKYRDEKEVRGIFVSNCTIRNTTNGVRIKTWAASPPGQATRITFQNIVLD 338

Query: 275 SVRNPIIIDQTYGSKKKKAPSQVKVSNVEFKNIRGTTVSDVAVSLQCSSAVPCDGIKLEE 334
            VRNPIIIDQ YGSK KK+PSQVKVS+V+FKNIRGTT+S VAVSLQCS+A+PCDGIKLE+
Sbjct: 339 KVRNPIIIDQNYGSKTKKSPSQVKVSDVQFKNIRGTTISPVAVSLQCSAALPCDGIKLEQ 398

Query: 335 IDLAFNGSRLKHPFANSCLNAKITAIGKQNPPACV 370
           ID+AF+G+ LK PFANSCLNAKIT IGKQNPPACV
Sbjct: 399 IDVAFSGTHLKQPFANSCLNAKITTIGKQNPPACV 433

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BLAST of Lsi02G024310 vs. TrEMBL
Match: F6H557_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0028g01300 PE=3 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 5.6e-53
Identity = 133/339 (39.23%), Postives = 187/339 (55.16%), Query Frame = 1


Query: 59  ATTTPTPTTTAAAAAAN--VFDITTHGAKPDGTTDNTQAFMQTWVAACHSGGPAKVVFPA 118
           ATT+      +AA  +   +FD+T +GA+ DG +D +QA ++ W  AC S   + V+ P 
Sbjct: 8   ATTSLLLLLASAAEVSGDIIFDVTKYGARTDGNSDISQALLKAWGDACSSPVASTVMIPD 67

Query: 119 GTFLTGPLVYAGPCDGPMTVEIQGTVKATTDITEY-SSAEWILFESITGLSLIGSGTFDG 178
           GT+  G ++ AGPC  P+   +QGTV A  D + + + A WI F+ I   +L G G FDG
Sbjct: 68  GTYALGQIIIAGPCKAPINFVVQGTVMAPVDTSRFRAEAGWITFQQIDQFTLSGDGVFDG 127

Query: 179 QVNKV-----------------------QQGDQWIDGGDFFIEQQSLPHILGFKPQYKSQ 238
           Q   V                       + GD  +  GD   EQ ++  +          
Sbjct: 128 QGKIVWGTKCLSSAYCNQLPINTNTISFETGDDCVSIGDGS-EQINIQRVTC-------- 187

Query: 239 QSPHNSSSVGSLGKYNDEKEVNGIFVSNCTIKNTTNGVRIKTWAASPPGQATRITFQDIV 298
             P +  SVGSLGKY +E+ V GI V NCT+ NT NGVR+KTW AS  G A+ + F+DIV
Sbjct: 188 -GPGHGISVGSLGKYPNEEPVVGISVKNCTLTNTQNGVRVKTWPASHQGTASEMHFEDIV 247

Query: 299 LDSVRNPIIIDQTYGSKKK---KAPSQVKVSNVEFKNIRGTTVSDVAVSLQCSSAVPCDG 358
           +++V NPIIIDQ Y    +   K+PS++K+SNV F+NIRGTT + VAV L CS  VPC  
Sbjct: 248 MNNVGNPIIIDQEYCPHNQCNLKSPSRIKISNVSFRNIRGTTSTQVAVKLICSQGVPCQD 307

Query: 359 IKLEEIDLAFNGSRLKHPFANSCLNAKITAIGKQNPPAC 369
           ++L +I+L +NG+    P  + C N K   +G Q P  C
Sbjct: 308 VELGDINLEYNGN--DGPAMSQCKNIKPNVLGAQLPRIC 334

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BLAST of Lsi02G024310 vs. TrEMBL
Match: A0A061DSW2_THECC (Polygalacturonase 4, putative OS=Theobroma cacao GN=TCM_004700 PE=3 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 5.4e-48
Identity = 101/159 (63.52%), Postives = 120/159 (75.47%), Query Frame = 1


Query: 215 PHNSSSVGSLGKYNDEKEVNGIFVSNCTIKNTTNGVRIKTWAASPPGQATRITFQDIVLD 274
           P +  S+GSLGKY DE++V+GI V NCT+ NTTNGVRIKTW  SPP QA+ ITFQDIV+D
Sbjct: 266 PGHGISIGSLGKYKDEEDVSGIVVKNCTLSNTTNGVRIKTWPGSPPSQASSITFQDIVMD 325

Query: 275 SVRNPIIIDQTYGSKKKKAPSQVKVSNVEFKNIRGTTVSDVAVSLQCSSAVPCDGIKLEE 334
            V+NPIIIDQ YGS  K APS+VKVS+V +KNIRGT+ S VAVSL CSS VPC G++L +
Sbjct: 326 MVQNPIIIDQNYGS-HKSAPSRVKVSDVHYKNIRGTSKSPVAVSLSCSSEVPCQGVQLVD 385

Query: 335 IDLAFNGSRLKHPFAN-----SCLNAKITAIGKQNPPAC 369
           IDLA+   R      N     SCLN K+T+ GKQNPPAC
Sbjct: 386 IDLAYKSVRKSKRMQNSTLSASCLNVKVTSGGKQNPPAC 423

[Close]

BLAST of Lsi02G024310 vs. TrEMBL
Match: B9RT98_RICCO (Exopolygalacturonase clone GBGE184, putative OS=Ricinus communis GN=RCOM_0682230 PE=3 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.3e-46
Identity = 92/154 (59.74%), Postives = 121/154 (78.57%), Query Frame = 1


Query: 215 PHNSSSVGSLGKYNDEKEVNGIFVSNCTIKNTTNGVRIKTWAASPPGQATRITFQDIVLD 274
           P +  S+GSLGKY DEK+V GI V+NCT+KNT NG+RIKTW  SPP  AT +TFQDI++D
Sbjct: 261 PGHGISIGSLGKYPDEKDVRGILVTNCTLKNTDNGIRIKTWGGSPPSVATSLTFQDIIMD 320

Query: 275 SVRNPIIIDQTYGSKKKKAPSQVKVSNVEFKNIRGTTVSDVAVSLQCSSAVPCDGIKLEE 334
           +VR+PIIIDQ+YGS  K APS+VK+SNV++ NIRGTT S+V V +QCS  VPC+G++L  
Sbjct: 321 NVRHPIIIDQSYGS--KSAPSRVKISNVKYMNIRGTTNSEVGVDIQCSKQVPCEGVRLSN 380

Query: 335 IDLAFNGSRLKHPFANSCLNAKITAIGKQNPPAC 369
           I+L + G + K PF++SCLNA+++  G Q PP C
Sbjct: 381 INLKYIGGK-KLPFSSSCLNARVSYAGMQFPPPC 411

[Close]

BLAST of Lsi02G024310 vs. TAIR10
Match: AT3G14040.1 (AT3G14040.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 146.7 bits (369), Expect = 2.7e-35
Identity = 87/208 (41.83%), Postives = 120/208 (57.69%), Query Frame = 1


Query: 164 GLSLIGSGTFDGQVNKVQQGDQWIDGGDFFIEQQSLPHILGFKPQYKSQQSPHNSSSVGS 223
           G++LIG+        K++ GD  +  GD   E   + ++         +  P +  S+GS
Sbjct: 250 GVNLIGA--------KIKTGDDCVSIGDG-TENLIVENV---------ECGPGHGISIGS 309

Query: 224 LGKYNDEKEVNGIFVSNCTIKNTTNGVRIKTWAASPPGQATRITFQDIVLDSVRNPIIID 283
           LG+Y +E+ V G+ V  C IKNT NGVRIKTW  SPPG A+ I F+DI +D+V  P++ID
Sbjct: 310 LGRYPNEQPVKGVTVRKCLIKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLID 369

Query: 284 Q---TYGSKKKKAPSQVKVSNVEFKNIRGTTVSDVAVSLQCSSAVPCDGIKLEEIDLAFN 343
           Q    YG  K   PS+VK+S+V  KNI+GT+ + VAV L CS  VPC  I L +I+L  N
Sbjct: 370 QEYCPYGHCKAGVPSKVKLSDVTIKNIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHN 429

Query: 344 GSRLKHPFANSCLNAKITAIGKQNPPAC 369
           G   + P  ++C N K    GK  P AC
Sbjct: 430 GK--EGPAVSACSNIKPILSGKLVPAAC 437

[Close]

BLAST of Lsi02G024310 vs. TAIR10
Match: AT3G07850.1 (AT3G07850.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 146.0 bits (367), Expect = 4.7e-35
Identity = 87/208 (41.83%), Postives = 119/208 (57.21%), Query Frame = 1


Query: 164 GLSLIGSGTFDGQVNKVQQGDQWIDGGDFFIEQQSLPHILGFKPQYKSQQSPHNSSSVGS 223
           G++LIG+        K++ GD  +  GD   E   + ++         +  P +  S+GS
Sbjct: 249 GVNLIGA--------KIKTGDDCVSIGDG-TENLIVENV---------ECGPGHGISIGS 308

Query: 224 LGKYNDEKEVNGIFVSNCTIKNTTNGVRIKTWAASPPGQATRITFQDIVLDSVRNPIIID 283
           LG+Y +E+ V G+ V  C IKNT NGVRIKTW  SPPG A+ I F+DI +D+V  P++ID
Sbjct: 309 LGRYPNEQPVKGVTVRKCLIKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLID 368

Query: 284 Q---TYGSKKKKAPSQVKVSNVEFKNIRGTTVSDVAVSLQCSSAVPCDGIKLEEIDLAFN 343
           Q    YG  K   PSQVK+S+V  K I+GT+ + VAV L CS  VPC  I L +I+L  N
Sbjct: 369 QEYCPYGHCKAGVPSQVKLSDVTIKGIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHN 428

Query: 344 GSRLKHPFANSCLNAKITAIGKQNPPAC 369
           G   + P  ++C N K    GK  P AC
Sbjct: 429 GK--EGPAVSACSNIKPILSGKLVPAAC 436

[Close]

BLAST of Lsi02G024310 vs. TAIR10
Match: AT4G18180.1 (AT4G18180.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 140.2 bits (352), Expect = 2.6e-33
Identity = 77/167 (46.11%), Postives = 104/167 (62.28%), Query Frame = 1


Query: 215 PHNSSSVGSLGKYNDEKEVNGIFVSNCTIKNTTNGVRIKTWAASPP-GQATRITFQDIVL 274
           P +  SVGSLG+Y +EK+VNG+ V +C I  TTNG+RIKTWA SP    AT +TF++I++
Sbjct: 247 PGHGISVGSLGRYPNEKDVNGLVVKDCKISGTTNGIRIKTWANSPGLSAATNMTFENIIM 306

Query: 275 DSVRNPIIIDQTY---GSKKKKAPSQVKVSNVEFKNIRGTTVSDVAVSLQCSSAVPCDGI 334
           ++V NPIIIDQ+Y    S     PS+V++S + FKNIRGT+ S VAV L CS  +PC  +
Sbjct: 307 NNVTNPIIIDQSYCPFSSCISNVPSKVELSEIYFKNIRGTSSSLVAVQLHCSRGMPCKKV 366

Query: 335 KLEEIDLAFNGS---------RLKHPFANSCLNAKITAIGKQNPPAC 369
            LE + L  + S         R     ++SC N +   IG Q PP C
Sbjct: 367 YLENVHLDLSSSDGGRKQSSNRGNEAVSSSCRNVRANYIGTQIPPPC 413

[Close]

BLAST of Lsi02G024310 vs. TAIR10
Match: AT1G02790.1 (AT1G02790.1 polygalacturonase 4)

HSP 1 Score: 139.8 bits (351), Expect = 3.3e-33
Identity = 72/161 (44.72%), Postives = 104/161 (64.60%), Query Frame = 1


Query: 215 PHNSSSVGSLGKYNDEKEVNGIFVSNCTIKNTTNGVRIKTWAASPPGQATRITFQDIVLD 274
           P +  SVGSLGKY +E++V+GI V+NCT+  T NG+RIKTW  S P +A  I F++I++ 
Sbjct: 262 PGHGLSVGSLGKYKNEEDVSGIHVNNCTMIETDNGLRIKTWGGSDPSKAVDIKFENIIMQ 321

Query: 275 SVRNPIIIDQTYGSKKKKAPSQVKVSNVEFKNIRGTTVSDVAVSLQCSSAVPCDGIKLEE 334
           SV+NPIIIDQ YGS  +   SQV +S++ FKNIRGTT++   V + CS +VPC G+ + +
Sbjct: 322 SVKNPIIIDQNYGS--RGGDSQVAISDILFKNIRGTTITKDVVQIMCSKSVPCQGVNVVD 381

Query: 335 IDLAFNGSRLKHPFANS-------CLNAKITAIGKQNPPAC 369
           ++L + G       ++S       C NA +   GK + P C
Sbjct: 382 VNLDYVGKTGGEKKSSSGGLVGALCDNANVIFGGKLSFPMC 420

[Close]

BLAST of Lsi02G024310 vs. TAIR10
Match: AT3G07820.1 (AT3G07820.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 128.6 bits (322), Expect = 7.7e-30
Identity = 69/158 (43.67%), Postives = 98/158 (62.03%), Query Frame = 1


Query: 215 PHNSSSVGSLGKYNDEKEVNGIFVSNCTIKNTTNGVRIKTW-AASPPGQATRITFQDIVL 274
           P +  SVGSLG+Y  E++V+GI V NCT++ T NG+RIKTW +A+    A+ I F+DI+L
Sbjct: 231 PGHGISVGSLGRYGHEQDVSGIKVINCTLQETDNGLRIKTWPSAACSTTASDIHFEDIIL 290

Query: 275 DSVRNPIIIDQTY---GSKKKKAPSQVKVSNVEFKNIRGTTVSDVAVSLQCSSAVPCDGI 334
             V NPI+IDQ Y       K+  S +K+ N+ FKNIRGT+ +  AV L CS   PC  +
Sbjct: 291 KDVSNPILIDQEYCPWNQCNKQKASTIKLVNISFKNIRGTSGNKDAVKLLCSKGYPCQNV 350

Query: 335 KLEEIDLAFNGSRLKHPFANSCLNAKITAIGKQNPPAC 369
           ++ +ID+ +NG+    P    C N     +G Q+P AC
Sbjct: 351 EIGDIDIKYNGA--DGPATFHCSNVSPKILGSQSPKAC 386

[Close]

BLAST of Lsi02G024310 vs. NCBI nr
Match: gi|743924680|ref|XP_011006467.1| (PREDICTED: exopolygalacturonase clone GBGE184-like [Populus euphratica])

HSP 1 Score: 310.1 bits (793), Expect = 5.3e-81
Identity = 167/314 (53.18%), Postives = 214/314 (68.15%), Query Frame = 1


Query: 70  AAAAANVFDITTHGAKPDGTTDNTQAFMQTWVAACHSGGPAKVVFPAGTFLTGPLVYAGP 129
           + AA  VFD+T HGAK D  TDN QAF+QTW AAC SG PAKVV P GTFLT P+V+ GP
Sbjct: 98  SGAAVTVFDVTKHGAKADDKTDNAQAFIQTWRAACDSGVPAKVVIPGGTFLTSPVVFQGP 157

Query: 130 CDG-PMTVEIQGTVKATTDITEYSSAEWILFESITGLSLIGSGTFDGQVNKVQQGDQWID 189
           C   P+  E+QG VKATTD++EYSS +WILFE I GL+L G GTFDGQ + V + +   +
Sbjct: 158 CKSKPIVFEVQGNVKATTDLSEYSSEQWILFEIIDGLTLNGGGTFDGQGSAVWKYNDCHE 217

Query: 190 G---------------GDFFIEQQSLPHILGFKPQYKSQQSPHNS--SSVGSLGKYNDEK 249
                            + F+ + S      F     S   P ++  S VGSLGKY +E+
Sbjct: 218 NKQCQALPSSIKLSKVNNAFVHEISSVDSKYFHMHVTSCPPPPSNPMSDVGSLGKYKNEE 277

Query: 250 EVNGIFVSNCTIKNTTNGVRIKTWAASPPGQATRITFQDIVLDSVRNPIIIDQTYGSKKK 309
           +V+GI V+NCT+ NTTNGVRIK++AAS P QA  ITF+DI +DSV+NPIIIDQ YGS + 
Sbjct: 278 DVSGIVVTNCTLFNTTNGVRIKSYAASDPSQALNITFKDITMDSVKNPIIIDQKYGS-RN 337

Query: 310 KAPSQVKVSNVEFKNIRGTTVSDVAVSLQCSSAVPCDGIKLEEIDLAFNGSRLKHPFANS 366
            APS+VK+SNV ++NI+GT+ S+VAVS  CSS VPC G++L +IDLA+ G +   P + S
Sbjct: 338 GAPSRVKISNVHYQNIKGTSTSNVAVSFLCSSLVPCQGVELVDIDLAYIGQKANMPLSAS 397

[Close]

BLAST of Lsi02G024310 vs. NCBI nr
Match: gi|802621092|ref|XP_012075836.1| (PREDICTED: exopolygalacturonase clone GBGE184-like [Jatropha curcas])

HSP 1 Score: 283.5 bits (724), Expect = 5.3e-73
Identity = 168/354 (47.46%), Postives = 215/354 (60.73%), Query Frame = 1


Query: 16  AVRWLVLGL-ACLSSMAQCSSAVAPNYSGDFSGIRGRLLVGAAPATTTPTPTTTAAAAAA 75
           A+ +LVL L +CL++ A   + V         G+R R  +G              + AA 
Sbjct: 11  AITFLVLALFSCLANGALSHTIV---------GLRNRRALGET------------SGAAI 70

Query: 76  NVFDITTHGAKPDGTTDNTQAFMQTWVAACHSGGPAKVVFPAGTFLTGPLVYAGPCDGPM 135
            VFD+T HGAK D  T+N  +F+QTW AAC S  PAKVV P GTFLTGP ++ GPC GP+
Sbjct: 71  TVFDVTEHGAKADDKTNNAMSFIQTWKAACASTAPAKVVIPDGTFLTGPTIFQGPCAGPV 130

Query: 136 TVEIQGTVKATTDITEYSSAEWILFESITGLSLIGSGTFDGQVNKVQQGDQWIDGGDFFI 195
             E+QG VKA+TD++EY+S EW+ FESI GL+  G GTF GQ   V + +      D   
Sbjct: 131 IFEVQGCVKASTDLSEYTSPEWMTFESIDGLTFTGKGTFSGQGPVVWEYNDCKSNEDC-- 190

Query: 196 EQQSLPHILGFKPQYKSQQSPHNSSSVGSLGKYNDEKEVNGIFVSNCTIKNTTNGVRIKT 255
             Q LP                 S SVGSLGKY DE +V+GI V NCT  NTTNG RIKT
Sbjct: 191 --QKLP----------------ASLSVGSLGKYPDEDDVSGILVINCTFSNTTNGARIKT 250

Query: 256 WAASPPGQATRITFQDIVLDSVRNPIIIDQTYGSKKKKAP---SQVKVSNVEFKNIRGTT 315
           ++ S P  A  I +QDI+++ V+NPIIIDQ YG K K+A    S+VKVS+V FKNI+GT+
Sbjct: 251 YSDSGPSNAFGIVYQDIIMNDVKNPIIIDQKYGIKDKEAQPSNSKVKVSDVHFKNIKGTS 310

Query: 316 VSDVAVSLQCSSAVPCDGIKLEEIDLAFNGSRLKHPFANSCLNAKITAIGKQNP 366
           +S  AV LQCSSA PC+G++L +IDL + G+      A  C NAK+T  G QNP
Sbjct: 311 ISAAAVDLQCSSAFPCEGVELSDIDLTYIGNEPSTESA-LCSNAKVTCDGIQNP 322

[Close]

BLAST of Lsi02G024310 vs. NCBI nr
Match: gi|659101323|ref|XP_008451547.1| (PREDICTED: exopolygalacturonase clone GBGE184 [Cucumis melo])

HSP 1 Score: 269.6 bits (688), Expect = 7.9e-69
Identity = 130/155 (83.87%), Postives = 143/155 (92.26%), Query Frame = 1


Query: 215 PHNSSSVGSLGKYNDEKEVNGIFVSNCTIKNTTNGVRIKTWAASPPGQATRITFQDIVLD 274
           P +  SVGSLGKY DEKEV GIFVSNCTI+NTTNGVRIKTWAASPPGQATRITFQDIVLD
Sbjct: 275 PGHGISVGSLGKYKDEKEVRGIFVSNCTIRNTTNGVRIKTWAASPPGQATRITFQDIVLD 334

Query: 275 SVRNPIIIDQTYGSKKKKAPSQVKVSNVEFKNIRGTTVSDVAVSLQCSSAVPCDGIKLEE 334
            VRNPIIIDQTYGSKKK++PSQVK+S+V+FKNIRGTT+S VAVSLQCS+A+PCD IKLE+
Sbjct: 335 KVRNPIIIDQTYGSKKKRSPSQVKISDVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQ 394

Query: 335 IDLAFNGSRLKHPFANSCLNAKITAIGKQNPPACV 370
           ID+AF+GSR K PF NSCLNAKIT IGKQNPPACV
Sbjct: 395 IDVAFSGSRSKQPFGNSCLNAKITTIGKQNPPACV 429

[Close]

BLAST of Lsi02G024310 vs. NCBI nr
Match: gi|778728116|ref|XP_011659369.1| (PREDICTED: exopolygalacturonase clone GBGE184-like [Cucumis sativus])

HSP 1 Score: 268.1 bits (684), Expect = 2.3e-68
Identity = 130/155 (83.87%), Postives = 143/155 (92.26%), Query Frame = 1


Query: 215 PHNSSSVGSLGKYNDEKEVNGIFVSNCTIKNTTNGVRIKTWAASPPGQATRITFQDIVLD 274
           P +  SVGSLGKY DEKEV GIFVSNCTI+NTTNGVRIKTWAASPPGQATRITFQ+IVLD
Sbjct: 279 PGHGISVGSLGKYRDEKEVRGIFVSNCTIRNTTNGVRIKTWAASPPGQATRITFQNIVLD 338

Query: 275 SVRNPIIIDQTYGSKKKKAPSQVKVSNVEFKNIRGTTVSDVAVSLQCSSAVPCDGIKLEE 334
            VRNPIIIDQ YGSK KK+PSQVKVS+V+FKNIRGTT+S VAVSLQCS+A+PCDGIKLE+
Sbjct: 339 KVRNPIIIDQNYGSKTKKSPSQVKVSDVQFKNIRGTTISPVAVSLQCSAALPCDGIKLEQ 398

Query: 335 IDLAFNGSRLKHPFANSCLNAKITAIGKQNPPACV 370
           ID+AF+G+ LK PFANSCLNAKIT IGKQNPPACV
Sbjct: 399 IDVAFSGTHLKQPFANSCLNAKITTIGKQNPPACV 433

[Close]

BLAST of Lsi02G024310 vs. NCBI nr
Match: gi|731409339|ref|XP_010657162.1| (PREDICTED: polygalacturonase [Vitis vinifera])

HSP 1 Score: 216.9 bits (551), Expect = 6.1e-53
Identity = 136/335 (40.60%), Postives = 190/335 (56.72%), Query Frame = 1


Query: 59  ATTTPTPTTTAAAAAAN--VFDITTHGAKPDGTTDNTQAFMQTWVAACHSGGPAKVVFPA 118
           ATT+      +AA  +   +FD+T +GA+ DG +D +QA ++ W  AC S   + V+ P 
Sbjct: 8   ATTSLLLLLASAAEVSGDIIFDVTKYGARTDGNSDISQALLKAWGDACSSPVASTVMIPD 67

Query: 119 GTFLTGPLVYAGPCDGPMTVEIQGTVKATTDITEY-SSAEWILFESITGLSLIGSGTFDG 178
           GT+  G ++ AGPC  P+   +QGTV A  D + + + A WI F+ I   +L G G FDG
Sbjct: 68  GTYALGQIIIAGPCKAPINFVVQGTVMAPVDTSRFRAEAGWITFQQIDQFTLSGDGVFDG 127

Query: 179 QVNKVQQGDQWIDGGDFFIEQQSLPHILGFKPQYK-----------SQQ--------SPH 238
           Q  K+  G + +     +  Q  LP  L F    K           S+Q         P 
Sbjct: 128 Q-GKIVWGTKCLSSA--YCNQ--LPINLRFNFITKTGDDCVSIGDGSEQINIQRVTCGPG 187

Query: 239 NSSSVGSLGKYNDEKEVNGIFVSNCTIKNTTNGVRIKTWAASPPGQATRITFQDIVLDSV 298
           +  SVGSLGKY +E+ V GI V NCT+ NT NGVR+KTW AS  G A+ + F+DIV+++V
Sbjct: 188 HGISVGSLGKYPNEEPVVGISVKNCTLTNTQNGVRVKTWPASHQGTASEMHFEDIVMNNV 247

Query: 299 RNPIIIDQTYGSKKK---KAPSQVKVSNVEFKNIRGTTVSDVAVSLQCSSAVPCDGIKLE 358
            NPIIIDQ Y    +   K+PS++K+SNV F+NIRGTT + VAV L CS  VPC  ++L 
Sbjct: 248 GNPIIIDQEYCPHNQCNLKSPSRIKISNVSFRNIRGTTSTQVAVKLICSQGVPCQDVELG 307

Query: 359 EIDLAFNGSRLKHPFANSCLNAKITAIGKQNPPAC 369
           +I+L +NG+    P  + C N K   +G Q P  C
Sbjct: 308 DINLEYNGN--DGPAMSQCKNIKPNVLGAQLPRIC 335

The following BLAST results are available for this feature:

BLAST of Lsi02G024310 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PGLR2_PLAAC	1.6e-37	49.35	Exopolygalacturonase (Fragment) OS=Platanus acerifolia GN=plaa2 PE=1 SV=1	[more]
PGLR_TOBAC	1.5e-35	50.32	Polygalacturonase OS=Nicotiana tabacum GN=PG1 PE=2 SV=1	[more]
PGLR2_ARATH	8.3e-34	41.83	Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana GN=At3g07850 PE=2 SV=...	[more]
PGLR_OENOR	1.4e-33	47.77	Exopolygalacturonase (Fragment) OS=Oenothera organensis PE=2 SV=1	[more]
PGLR_GOSBA	9.2e-33	45.86	Polygalacturonase OS=Gossypium barbadense GN=G9 PE=2 SV=1	[more]

BLAST of Lsi02G024310 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A067KRA0_JATCU	3.7e-73	47.46	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_10580 PE=3 SV=1	[more]
A0A0A0K9C4_CUCSA	1.6e-68	83.87	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G404800 PE=3 SV=1	[more]
F6H557_VITVI	5.6e-53	39.23	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0028g01300 PE=3 SV=...	[more]
A0A061DSW2_THECC	5.4e-48	63.52	Polygalacturonase 4, putative OS=Theobroma cacao GN=TCM_004700 PE=3 SV=1	[more]
B9RT98_RICCO	1.3e-46	59.74	Exopolygalacturonase clone GBGE184, putative OS=Ricinus communis GN=RCOM_0682230...	[more]

BLAST of Lsi02G024310 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G14040.1	2.7e-35	41.83	Pectin lyase-like superfamily protein	[more]
AT3G07850.1	4.7e-35	41.83	Pectin lyase-like superfamily protein	[more]
AT4G18180.1	2.6e-33	46.11	Pectin lyase-like superfamily protein	[more]
AT1G02790.1	3.3e-33	44.72	polygalacturonase 4	[more]
AT3G07820.1	7.7e-30	43.67	Pectin lyase-like superfamily protein	[more]

BLAST of Lsi02G024310 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|743924680\|ref\|XP_011006467.1\|	5.3e-81	53.18	PREDICTED: exopolygalacturonase clone GBGE184-like [Populus euphratica]	[more]
gi\|802621092\|ref\|XP_012075836.1\|	5.3e-73	47.46	PREDICTED: exopolygalacturonase clone GBGE184-like [Jatropha curcas]	[more]
gi\|659101323\|ref\|XP_008451547.1\|	7.9e-69	83.87	PREDICTED: exopolygalacturonase clone GBGE184 [Cucumis melo]	[more]
gi\|778728116\|ref\|XP_011659369.1\|	2.3e-68	83.87	PREDICTED: exopolygalacturonase clone GBGE184-like [Cucumis sativus]	[more]
gi\|731409339\|ref\|XP_010657162.1\|	6.1e-53	40.60	PREDICTED: polygalacturonase [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0004650	polygalacturonase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR012334	Pectin_lyas_fold
IPR011050	Pectin_lyase_fold/virulence
IPR000743	Glyco_hydro_28

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071555	cell wall organization
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004650	polygalacturonase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G024310.1	Lsi02G024310.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 104..203 score: 3.2E-10coord: 220..341 score: 2.3
IPR011050	Pectin lyase fold/virulence factor	unknown	SSF51126	Pectin lyase-like	coord: 67..369 score: 8.17
IPR012334	Pectin lyase fold	GENE3D	G3DSA:2.160.20.10		coord: 217..368 score: 1.0E-44coord: 64..201 score: 2.4
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 3..369 score: 1.8E
None	No IPR available	PANTHER	PTHR31375:SF16	SUBFAMILY NOT NAMED	coord: 3..369 score: 1.8E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi02G024310	CmaCh17G010690	Cucurbita maxima (Rimu)	cmalsiB348
Lsi02G024310	ClCG09G005740	Watermelon (Charleston Gray)	lsiwcgB159
Lsi02G024310	MELO3C015966.2	Melon (DHL92) v3.6.1	lsimedB201
Lsi02G024310	Cla97C09G168050	Watermelon (97103) v2	lsiwmbB212
Lsi02G024310	Bhi09G000749	Wax gourd	lsiwgoB271
Lsi02G024310	CsGy7G016220	Cucumber (Gy14) v2	cgyblsiB472
Lsi02G024310	Carg07158	Silver-seed gourd	carlsiB098
Lsi02G024310	CsaV3_7G029560	Cucumber (Chinese Long) v3	cuclsiB538

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi02G024310	Bottle gourd (USVL1VR-Ls)	lsilsiB091
Lsi02G024310	Cucumber (Gy14) v1	cgylsiB110
Lsi02G024310	Cucumber (Gy14) v1	cgylsiB351
Lsi02G024310	Cucurbita maxima (Rimu)	cmalsiB822
Lsi02G024310	Cucurbita moschata (Rifu)	cmolsiB337
Lsi02G024310	Cucurbita moschata (Rifu)	cmolsiB809
Lsi02G024310	Cucurbita pepo (Zucchini)	cpelsiB113
Lsi02G024310	Cucurbita pepo (Zucchini)	cpelsiB262
Lsi02G024310	Wild cucumber (PI 183967)	cpilsiB266
Lsi02G024310	Wild cucumber (PI 183967)	cpilsiB517
Lsi02G024310	Cucumber (Chinese Long) v2	culsiB267
Lsi02G024310	Cucumber (Chinese Long) v2	culsiB503
Lsi02G024310	Melon (DHL92) v3.5.1	lsimeB192
Lsi02G024310	Melon (DHL92) v3.5.1	lsimeB226
Lsi02G024310	Watermelon (Charleston Gray)	lsiwcgB162
Lsi02G024310	Watermelon (97103) v1	lsiwmB175
Lsi02G024310	Watermelon (97103) v1	lsiwmB178
Lsi02G024310	Silver-seed gourd	carlsiB511
Lsi02G024310	Cucumber (Chinese Long) v3	cuclsiB287
Lsi02G024310	Watermelon (97103) v2	lsiwmbB162
Lsi02G024310	Watermelon (97103) v2	lsiwmbB188
Lsi02G024310	Wax gourd	lsiwgoB202
Lsi02G024310	Wax gourd	lsiwgoB218