Lsi02G018730 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi02G018730
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionLong-chain-alcohol O-fatty-acyltransferase
Locationchr02 : 24529337 .. 24530471 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGAGTTTATGATTTAACACAAATGTCGATGAAAATATCGATGTCAAACGGATAGAAATGTACGTGAAAATTTATTATCATGTACCAAACTCACTCAAAATCTAAGTATAAATAAAAAAAATGGCACAACTTTTGAAAAGCAAAAACAAAAAACCAAAAGAAGCTAAGCTAAAAAAACCTCCAATATGGATGGAGAAATCATTAGATTTATCAAGGTGTTGCTCATATGCATGGCATCTCTATTGTATTGCTATTCCATAGCTTCAAAACTTCCCAAAGGCAAACTCAGGCTCCTTTCTCTCCTCCCAATCTTCTCTCTCTTCGCCGTCCTTCCTCTCGACCTCTCCTACGTCGTCCTCACGAGCGGCATCACCATATTCGTCACCTGGCTTACGACCTTTAAGCTTCTTCAATTCTCATTCGATTTGGGTCCTCTAGCTTCCAACCCACCATTGTCATTCCCTCTTTTTGCCTCCATTGCTTTCTTACCAACTAGAATCAAGGATATCAAAACAACCCCATATGTTCAAAAACATAGAAAAGATTCAAATGCACCTCAAGAACTTGACCAAGAACCACCACCCAAGTTGCCTCTAAACTTGCCAGCTAAAGTGTTGTTATTTGCATTATTGGTCTTGGGCAAGAGCCATGTTGATCTTGTCCACCCCATTGTAAAATTATGCCTCAATTGTGGAATATTGTATTTCTACTTAGACATGGTTATGAGTTTGTCTAATGTTTTTGTTCGATCGAGCTTCGGTGTTGAGGTCAGACGACCGTTTGACGAGCCATATCTCGCAACATCACTTCAAAATTTTTGGGGTCGGAGGTGGAATCGATTAGTATCTGAAACTTTGCACAACACCATCTATAAACCAGTTCGTTACGACGTCGGGAGCCCGCGATGGATGGCAGTGTTCGTAGTGTTTGTGGTGTCGGGGTTAATGCATGAGCTTTTGTTCTACTACATCATCAGAGTGGTTCCGACGTGGGAAGTCACATGGTTCTTTATTATTCACGGAGTGTGTGTGGCCATGGAGATTGAATTAAAGAGTGCGTTAGGGAAAAAATGGCAATTTCATTGGGCAGTGACCGGACCACTCACGATGACATTTATAGCAATCACCGCTCAT

mRNA sequence

ATGCGAGTGTTGCTCATATGCATGGCATCTCTATTGTATTGCTATTCCATAGCTTCAAAACTTCCCAAAGGCAAACTCAGGCTCCTTTCTCTCCTCCCAATCTTCTCTCTCTTCGCCGTCCTTCCTCTCGACCTCTCCTACGTCGTCCTCACGAGCGGCATCACCATATTCGTCACCTGGCTTACGACCTTTAAGCTTCTTCAATTCTCATTCGATTTGGGTCCTCTAGCTTCCAACCCACCATTGTCATTCCCTCTTTTTGCCTCCATTGCTTTCTTACCAACTAGAATCAAGGATATCAAAACAACCCCATATGTTCAAAAACATAGAAAAGATTCAAATGCACCTCAAGAACTTGACCAAGAACCACCACCCAAGTTGCCTCTAAACTTGCCAGCTAAAGTGTTGTTATTTGCATTATTGGTCTTGGGCAAGAGCCATGTTGATCTTGTCCACCCCATTGTAAAATTATGCCTCAATTGTGGAATATTGTATTTCTACTTAGACATGGTTATGAGTTTGTCTAATGTTTTTGTTCGATCGAGCTTCGGTGTTGAGGTCAGACGACCGTTTGACGAGCCATATCTCGCAACATCACTTCAAAATTTTTGGGGTCGGAGGTGGAATCGATTAGTATCTGAAACTTTGCACAACACCATCTATAAACCAGTTCGTTACGACGTCGGGAGCCCGCGATGGATGGCAGTGTTCGTAGTGTTTGTGGTGTCGGGGTTAATGCATGAGCTTTTGTTCTACTACATCATCAGAGTGGTTCCGACGTGGGAAGTCACATGGTTCTTTATTATTCACGGAGTGTGTGTGGCCATGGAGATTGAATTAAAGAGTGCGTTAGGGAAAAAATGGCAATTTCATTGGGCAGTGACCGGACCACTCACGATGACATTTATAGCAATCACCGCTCAT

Coding sequence (CDS)

ATGCGAGTGTTGCTCATATGCATGGCATCTCTATTGTATTGCTATTCCATAGCTTCAAAACTTCCCAAAGGCAAACTCAGGCTCCTTTCTCTCCTCCCAATCTTCTCTCTCTTCGCCGTCCTTCCTCTCGACCTCTCCTACGTCGTCCTCACGAGCGGCATCACCATATTCGTCACCTGGCTTACGACCTTTAAGCTTCTTCAATTCTCATTCGATTTGGGTCCTCTAGCTTCCAACCCACCATTGTCATTCCCTCTTTTTGCCTCCATTGCTTTCTTACCAACTAGAATCAAGGATATCAAAACAACCCCATATGTTCAAAAACATAGAAAAGATTCAAATGCACCTCAAGAACTTGACCAAGAACCACCACCCAAGTTGCCTCTAAACTTGCCAGCTAAAGTGTTGTTATTTGCATTATTGGTCTTGGGCAAGAGCCATGTTGATCTTGTCCACCCCATTGTAAAATTATGCCTCAATTGTGGAATATTGTATTTCTACTTAGACATGGTTATGAGTTTGTCTAATGTTTTTGTTCGATCGAGCTTCGGTGTTGAGGTCAGACGACCGTTTGACGAGCCATATCTCGCAACATCACTTCAAAATTTTTGGGGTCGGAGGTGGAATCGATTAGTATCTGAAACTTTGCACAACACCATCTATAAACCAGTTCGTTACGACGTCGGGAGCCCGCGATGGATGGCAGTGTTCGTAGTGTTTGTGGTGTCGGGGTTAATGCATGAGCTTTTGTTCTACTACATCATCAGAGTGGTTCCGACGTGGGAAGTCACATGGTTCTTTATTATTCACGGAGTGTGTGTGGCCATGGAGATTGAATTAAAGAGTGCGTTAGGGAAAAAATGGCAATTTCATTGGGCAGTGACCGGACCACTCACGATGACATTTATAGCAATCACCGCTCAT

Protein sequence

MRVLLICMASLLYCYSIASKLPKGKLRLLSLLPIFSLFAVLPLDLSYVVLTSGITIFVTWLTTFKLLQFSFDLGPLASNPPLSFPLFASIAFLPTRIKDIKTTPYVQKHRKDSNAPQELDQEPPPKLPLNLPAKVLLFALLVLGKSHVDLVHPIVKLCLNCGILYFYLDMVMSLSNVFVRSSFGVEVRRPFDEPYLATSLQNFWGRRWNRLVSETLHNTIYKPVRYDVGSPRWMAVFVVFVVSGLMHELLFYYIIRVVPTWEVTWFFIIHGVCVAMEIELKSALGKKWQFHWAVTGPLTMTFIAITAH
BLAST of Lsi02G018730 vs. Swiss-Prot
Match: WAXS1_ARATH (Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana GN=AT1 PE=2 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 2.7e-46
Identity = 125/314 (39.81%), Postives = 175/314 (55.73%), Query Frame = 1

Query: 1   MRVLLICMASLLYCYSIASKLPKGKLRLLSLLPIFSLFAVLPLDLSYVVLTSGITIFVTW 60
           + V +  + SL YCY I+SKL KG LRLLS+LP+  LF VLPL LS V   +   +F++W
Sbjct: 9   IEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCAISVLFLSW 68

Query: 61  LTTFKLLQFSFDLGPLASNPPLSFPLFASIAFLPTRIKDIKTTPYVQKHRKDSNAPQELD 120
           L  FKLL F+FD GPL   PP     F   A LP +I+   +           NA   L 
Sbjct: 69  LANFKLLLFAFDEGPLFPLPP-KLSRFICFACLPIKIRQDPSP----------NAIPNLH 128

Query: 121 QEPPPKLPLNLPAKVLLFALLVLGKSHVDLVHPIVKLCLNCGILYFYLDMVMSLSNVFVR 180
            +P PK  L +  K+L+  +L+    + D +   V L L C  +Y  +++V+      V 
Sbjct: 129 PKPMPKWVLAV--KILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVVS 188

Query: 181 SSFGVEVRRPFDEPYLATSLQNFWGRRWNRLVSETLHNTIYKPVR------YDVGSPRWM 240
           +  G  +   F+EPYLATSLQ+FW RRWN +VS  L +T++ PV+             + 
Sbjct: 189 TLLGCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFA 248

Query: 241 AVFVVFVVSGLMHELLFYYIIRVVPTWEVTWFFIIHGVCVAMEIELKSALGKKW--QFHW 300
            V   F VSGLMHELL++Y+IR  PTWEVT FF++HG   A EI +K     +W    H 
Sbjct: 249 GVMASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRT---QWLRPPHR 306

Query: 301 AVTGPLTMTFIAIT 307
           AV+G + +TF+++T
Sbjct: 309 AVSGLVVLTFVSVT 306

BLAST of Lsi02G018730 vs. Swiss-Prot
Match: WAXS1_SIMCH (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis PE=1 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 4.6e-46
Identity = 122/314 (38.85%), Postives = 181/314 (57.64%), Query Frame = 1

Query: 3   VLLICMASLLYCYSIASKLPKG-KLRLLSLLPIFSLFAVLPLDLSYVVLTSGITIFVTWL 62
           V +  +A+  YC  + +  P G  LRLL LLP+  LF  LPL LS   L     +++ WL
Sbjct: 13  VWISAIAAACYCRFVPAVAPHGGALRLLLLLPVVLLFIFLPLRLSSFHLGGPTALYLVWL 72

Query: 63  TTFKLLQFSFDLGPLASNPPLSFPLFASIAFLPTRIKDIKTTPYVQKHRKDSNAPQELDQ 122
             FKLL F+F LGPL SNP LS   F S   LP + +D  +  + +  R  S   +++  
Sbjct: 73  ANFKLLLFAFHLGPL-SNPSLSLLHFISTTLLPIKFRDDPSNDHEKNKRTLSFEWRKV-- 132

Query: 123 EPPPKLPLNLPAKVLLFA-LLVLGKSHVDLVHPIVKLCLNCGILYFYLDMVMSLSNVFVR 182
                  +   AK++ FA +L + +   DL H ++ + L C   Y   ++ ++ S V  R
Sbjct: 133 -------VLFVAKLVFFAGILKIYEFRKDLPHFVISV-LYCFHFYLGTEITLAASAVIAR 192

Query: 183 SSFGVEVRRPFDEPYLATSLQNFWGRRWNRLVSETLHNTIYKPVRYDVGSPRWM------ 242
           ++ G+++   F+EPYLATSLQ+FWGRRWN +VS+ L  T Y+PVR  +   RW+      
Sbjct: 193 ATLGLDLYPQFNEPYLATSLQDFWGRRWNLMVSDILGLTTYQPVRRVLS--RWVRLRWEV 252

Query: 243 --AVFVVFVVSGLMHELLFYYIIRVVPTWEVTWFFIIHGVCVAMEIELKSALGKKWQFHW 302
             A+ V F VSGLMHE+ F+Y+ R  P+WEVT FF++HGVC A+E+ +K A+  K +   
Sbjct: 253 AGAMLVAFTVSGLMHEVFFFYLTRARPSWEVTGFFVLHGVCTAVEMVVKKAVSGKVRLRR 312

Query: 303 AVTGPLTMTFIAIT 307
            V+G LT+ F+ +T
Sbjct: 313 EVSGALTVGFVMVT 313

BLAST of Lsi02G018730 vs. Swiss-Prot
Match: ASAT1_ARATH (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 1.0e-45
Identity = 111/309 (35.92%), Postives = 175/309 (56.63%), Query Frame = 1

Query: 6   ICMASLLYCYSIASKLPKGKLRLLSLLPIFSLFAVLPLDLSYVVLTSGIT-IFVTWLTTF 65
           + + SL Y + IA  +PKG  RL+   P+F +F ++P  L Y +   GIT  F+ WL  F
Sbjct: 10  LVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPF-LIYSLHLLGITAFFIAWLANF 69

Query: 66  KLLQFSFDLGPLASN-PPLSFPLFASIAFLPTRIKDIKTTPYVQKHRKDSNAPQELDQEP 125
           KLL F+   GPL+SN  PLS P+F +++ LP +I+          H   +  P       
Sbjct: 70  KLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGP------- 129

Query: 126 PPKLPLNLPAKVLLFALLVLGKSHVDLVHPIVKLCLNCGILYFYLDMVMSLSNVFVRSSF 185
                L    K +   L++    +   +   V L L    +YF L+++++ +   VR+  
Sbjct: 130 -----LIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMS 189

Query: 186 GVEVRRPFDEPYLATSLQNFWGRRWNRLVSETLHNTIYKPVR--YDVGSPRW---MAVFV 245
            +E+   F++PYLATSLQ+FWGRRWN +V+  L  T+Y+P    + V  P +   +A F 
Sbjct: 190 DLELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFSVLGPNYSQILAAFG 249

Query: 246 VFVVSGLMHELLFYYIIRVVPTWEVTWFFIIHGVCVAMEIELKSALGKKWQFHWAVTGPL 305
            FVVSG+MHEL+F+Y+ R+ P W++ WFF+I+G C  +EI +K  +  +W+F  A++  L
Sbjct: 250 TFVVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVL 305

Query: 306 TMTFIAITA 308
           T+TF+ +TA
Sbjct: 310 TLTFVMVTA 305

BLAST of Lsi02G018730 vs. Swiss-Prot
Match: WAXSA_ARATH (Putative long-chain-alcohol O-fatty-acyltransferase 10 OS=Arabidopsis thaliana GN=At1g34490 PE=3 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 4.8e-43
Identity = 120/303 (39.60%), Postives = 170/303 (56.11%), Query Frame = 1

Query: 10  SLLYCYSIASKLPKGKLRLLSLLPIFSLFAVLPLDLSYVVLTSGITIFVTWLTTFKLLQF 69
           S+ YCY I SK+ +G  RLLS+LP+  LF VLPL   + + +S     ++ L  FKL+ F
Sbjct: 18  SVSYCYYIPSKIKRGVHRLLSVLPVCVLFLVLPLFFVFTIFSSTTAFCLSILANFKLILF 77

Query: 70  SFDLGPLASNPPLSFPLFASIAFLPTRIKDIKTTPYVQKHRKDSNAPQELDQEPPPKLPL 129
           +FD GPL    PL   LF  I F    IK ++T P  Q H               PK  L
Sbjct: 78  AFDKGPLL---PLPTNLFRFICFTCLPIK-LQTKPNSQNHL--------------PKWVL 137

Query: 130 NLPAKVLLFALLVLGKSHVDLVHPIVKLCLNCGILYFYLDMVMSLSNVFVRSSFGVEVRR 189
             P+KV +F LL+  +S+  L+ PI+ L L    LY  LD+++++ N  +      ++  
Sbjct: 138 --PSKVAIFVLLLNIRSYKILLPPILLLGLYPLHLYIVLDVLLTIVNALLTIILRCDLEP 197

Query: 190 PFDEPYLATSLQNFWGRRWNRLVSETLHNTIYKPVR----YDVGS--PRWMAVFVVFVVS 249
            F+EPYLATSLQ+FWG RWN +VS      +Y PVR    + + S   R+M     F VS
Sbjct: 198 HFNEPYLATSLQDFWGHRWNLMVSAIYRPGVYSPVRSVCQHQMRSDWARFMGCMTTFFVS 257

Query: 250 GLMHELLFYYIIRVVPTWEVTWFFIIHGVCVAMEIELKSALGKKWQFHWAVTGPLTMTFI 307
           GL+HEL+++YI R  PT EVTWFF++HGVC AMEI +K  +  +W     +   +T+ F+
Sbjct: 258 GLIHELVYFYINREKPTLEVTWFFVLHGVCTAMEIAVKRKM--QWSLSPMLLRLITVGFL 298

BLAST of Lsi02G018730 vs. Swiss-Prot
Match: WAXS5_ARATH (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana GN=AT5 PE=2 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 3.1e-42
Identity = 112/314 (35.67%), Postives = 173/314 (55.10%), Query Frame = 1

Query: 1   MRVLLICMASLLYCYSIASKLPKGKLRLLSLLPIFSLFAVLPLDLSYVVLTSGITIFVTW 60
           ++V +  + S+ YCY I  ++  G  R LS+ P+ +LF VLPL  S + L+     F+TW
Sbjct: 9   IKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSLITAFFLTW 68

Query: 61  LTTFKLLQFSFDLGPLASNPPLSFPLFASIAFLPTRIKDIKTTPYVQKHRKDSNAPQELD 120
           L  FKL+ FSFD GPL   P  +FP F      P +++     P  Q H           
Sbjct: 69  LANFKLILFSFDKGPLIPIPT-NFPRFFCFTCFPIKVQQ---NPKSQNHL---------- 128

Query: 121 QEPPPKLPLNLPAKVLLFALLVLGKSHVDLVHPIVKLCLNCGILYFYLDMVMSLSNVFVR 180
               PKL   +  K+ +FA+L+   S+   + P + L L    LY  ++++++   V V 
Sbjct: 129 ----PKLVFAI--KLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVVF 188

Query: 181 SSFGVEVRRPFDEPYLATSLQNFWGRRWNRLVSETLHNTIYKPVR------YDVGSPRWM 240
            S G ++    ++PYLATSLQ+FWGRRWN +V   L   +Y P+R         G   + 
Sbjct: 189 ISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALFP 248

Query: 241 AVFVVFVVSGLMHELLFYYIIRVVPTWEVTWFFIIHGVCVAME--IELKSALGKKWQFHW 300
            +   F+VSGL+HELLF+Y+IR +PT EVT FF++HGVC A+E  ++ K+ + ++W+   
Sbjct: 249 GILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLSP 302

Query: 301 AVTGPLTMTFIAIT 307
            V+  LT+ F+ +T
Sbjct: 309 GVSRVLTVGFVFVT 302

BLAST of Lsi02G018730 vs. TrEMBL
Match: A0A0A0K522_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G091730 PE=4 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 8.5e-156
Identity = 276/308 (89.61%), Postives = 293/308 (95.13%), Query Frame = 1

Query: 1   MRVLLICMASLLYCYSIASKLPKGKLRLLSLLPIFSLFAVLPLDLSYVVLTSGITIFVTW 60
           M+VLLICMASL+YCY IASKLPKGK RLLSLLPIFSLFAVLPLDLSYVVLTSGITIFVTW
Sbjct: 11  MKVLLICMASLMYCYYIASKLPKGKPRLLSLLPIFSLFAVLPLDLSYVVLTSGITIFVTW 70

Query: 61  LTTFKLLQFSFDLGPLASNPPLSFPLFASIAFLPTRIKDIKTTPYVQKHRKDSNAPQELD 120
           LTTFKL+QFSFDLGPLAS+PPLSFPLFASIAFLPTRIKDIKTTPYVQ H+KDSNAPQELD
Sbjct: 71  LTTFKLIQFSFDLGPLASDPPLSFPLFASIAFLPTRIKDIKTTPYVQNHKKDSNAPQELD 130

Query: 121 QEPPPKLPLNLPAKVLLFALLVLGKSHVDLVHPIVKLCLNCGILYFYLDMVMSLSNVFVR 180
           QE PPKLPLNLPAK  +FALLV+ K+HV LVHPI+KL LNCGILYFYLDM+MS+SN FVR
Sbjct: 131 QETPPKLPLNLPAKAFIFALLVICKNHVHLVHPILKLILNCGILYFYLDMIMSISNEFVR 190

Query: 181 SSFGVEVRRPFDEPYLATSLQNFWGRRWNRLVSETLHNTIYKPVRYDVGSPRWMAVFVVF 240
            SFG+EVRR FDEPYLATSLQNFWGRRWNRLVSETLHN IYKP+RYDVGSPRWMAVFVVF
Sbjct: 191 LSFGIEVRRSFDEPYLATSLQNFWGRRWNRLVSETLHNMIYKPIRYDVGSPRWMAVFVVF 250

Query: 241 VVSGLMHELLFYYIIRVVPTWEVTWFFIIHGVCVAMEIELKSALGKKWQFHWAVTGPLTM 300
           VVSGLMHELLFYYIIRVVPTWEVTWFF+IHGV VA+EIELKSALGKKWQFHWAVTGPLTM
Sbjct: 251 VVSGLMHELLFYYIIRVVPTWEVTWFFVIHGVFVALEIELKSALGKKWQFHWAVTGPLTM 310

Query: 301 TFIAITAH 309
            F+A+TAH
Sbjct: 311 AFMAVTAH 318

BLAST of Lsi02G018730 vs. TrEMBL
Match: A0A0A0K6W1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G091220 PE=4 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 1.4e-102
Identity = 200/307 (65.15%), Postives = 235/307 (76.55%), Query Frame = 1

Query: 1   MRVLLICMASLLYCYSIASKLPKGKLRLLSLLPIFSLFAVLPLDLSYVVLTSGITIFVTW 60
           ++VL + + S LYCY IASK  K KLRLLSLLP+FS+F VLPL  S ++ TS + +FVTW
Sbjct: 9   IKVLPVFILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTSSVGLFVTW 68

Query: 61  LTTFKLLQFSFDLGPLASNPPLSFPLFASIAFLPTRIKDIKTTPYVQKHRKDSNAPQELD 120
           LTTFKLL F+FD GPLASNPPLSFPLFASIAF PTR K  KTTP+ QK            
Sbjct: 69  LTTFKLLLFTFDSGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQKSL---------- 128

Query: 121 QEPPPKLPLNLPAKVLLFALLVLGKSHVDLVHPI-VKLCLNCGILYFYLDMVMSLSNVFV 180
              P  LPLNLP KV++ A+L+LG S++DLV P  VK  +N   +YFYLD+VMSLSNVFV
Sbjct: 129 ---PKLLPLNLPTKVVILAILILGNSYIDLVCPNNVKSWINGATVYFYLDVVMSLSNVFV 188

Query: 181 RSSFGVEVRRPFDEPYLATSLQNFWGRRWNRLVSETLHNTIYKPVRYDVGSPRWMAVFVV 240
           +S+FGVEVR+PF+EPYLATSLQN WGRRWN LVSETLH+TIYKP+RY VG PRW+AV  V
Sbjct: 189 QSTFGVEVRQPFNEPYLATSLQNLWGRRWNLLVSETLHSTIYKPIRYKVGMPRWVAVVTV 248

Query: 241 FVVSGLMHELLFYYIIRVVPTWEVTWFFIIHGVCVAMEIELKSALGKKWQFHWAVTGPLT 300
           FVVSGLMHELL+YY++R  PTWE TWFF+IHGVC+A+EIELK A  KKWQ   AV+  LT
Sbjct: 249 FVVSGLMHELLYYYMVRANPTWEFTWFFVIHGVCLALEIELKRAYEKKWQLQRAVSTLLT 302

Query: 301 MTFIAIT 307
             F   T
Sbjct: 309 TMFTGTT 302

BLAST of Lsi02G018730 vs. TrEMBL
Match: A0A0A0K576_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G179120 PE=4 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 6.2e-82
Identity = 171/313 (54.63%), Postives = 216/313 (69.01%), Query Frame = 1

Query: 2   RVLLICMASLLYCYSIASKLPKGKLRLLSLLPIFSLFAVLPLDLSYVVLTSGITIFVTWL 61
           +V L  M SLLY Y I+SKLPKGK RL+SL P FSLFA+LPL LS V L+  +  F+TWL
Sbjct: 13  KVSLTIMVSLLYSYFISSKLPKGKFRLISLFPTFSLFALLPLSLSSVFLSGTVAFFITWL 72

Query: 62  TTFKLLQFSFDLGPLASNPPLSFPLFASIAFLPTRIKDIKTTPYVQKHRKDSNAPQELDQ 121
           TTFKLL FSF+LGPL S+PPL+FPLF ++A LP +IK  +  P  +   K +N       
Sbjct: 73  TTFKLLLFSFNLGPLVSDPPLTFPLFVTVACLPIKIKPREIDPDYKYLEKYAN------- 132

Query: 122 EPPPKLPLNLPAKVLLFALLVLGKSHVDLVHPIVKLCLNCGILYFYLDMVMSLSNVFVRS 181
              PKL LNLPAK+LLFA+L+    H+D   P VK+C+ C  LY +++ ++ +S+  VRS
Sbjct: 133 ---PKLGLNLPAKILLFAILIAAGDHIDQFRPNVKICVYCVTLYLFVEFLLGVSSAIVRS 192

Query: 182 SF-GVEVRRPFDEPYLATSLQNFWGRRWNRLVSETLHNTIYKPVRYDVG---SPRWMA-- 241
           SF GV +    +EPYLATSLQ+FWGRRWN LVS TL   IY+PVR  VG     RW A  
Sbjct: 193 SFDGVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRPVRAAVGGVVGKRWAAGT 252

Query: 242 -VFVVFVVSGLMHELLFYYIIRVVPTWEVTWFFIIHGVCVAMEIELKSALGKKWQFHWAV 301
            V  VFVVSG+MHE L YYI R VPTWEVTWFF++ GVCV +E ELK+ +G++WQ   AV
Sbjct: 253 GVMAVFVVSGVMHEQLVYYITREVPTWEVTWFFVLQGVCVVLEFELKTVVGRRWQPPAAV 312

Query: 302 TGPLTMTFIAITA 308
              LT++F+ +TA
Sbjct: 313 AWLLTLSFLVVTA 315

BLAST of Lsi02G018730 vs. TrEMBL
Match: A0A0A0K8E9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G145990 PE=4 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 1.8e-73
Identity = 162/313 (51.76%), Postives = 209/313 (66.77%), Query Frame = 1

Query: 1   MRVLLICMASLLYCYSIASKLPKGKLRLLSLLPIFSLFAVLPLDLSYVVLTSGITIFVTW 60
           +++ L  +ASLLY Y I+SKLPKG  RLLSLLPI SLF +LPL +S ++  + ++  VTW
Sbjct: 12  LKIFLSVLASLLYSYFISSKLPKGLPRLLSLLPILSLFTLLPLLVSSILFAAIVSSIVTW 71

Query: 61  LTTFKLLQFSFDLGPLASNPPLSFPLFASIAFLPTRIKDIKTTPYVQKHRKDSNAPQELD 120
           LT+FKLL FSF  GPL S+PPL F LF S+AF P RIK           +K+S  P +  
Sbjct: 72  LTSFKLLLFSFHSGPLVSDPPLPFLLFVSLAFFPIRIK-----------KKESQKPID-- 131

Query: 121 QEPPPKLPLNLPAKVLLFALLVLGKSHVDLVHPIVKLCLNCGILYFYLDMVMSLSNVFVR 180
               PKLP+N PAKV LF +L+  +   D + P +KLC NC  LYF++D+V+   N   R
Sbjct: 132 ---SPKLPINFPAKVFLFTVLMAVE---DSLPPYMKLCFNCVALYFFIDIVVGFFNTIAR 191

Query: 181 SSFGVEVRRPFDEPYLATSLQNFWGRRWNRLVSETLHNTIYKPVR---YDVGSPRWMA-- 240
             FG+E   P +EPYL+TSLQ+FWGRRWN LVS  L  T++ PVR    DV   R  A  
Sbjct: 192 WVFGIEFEEPSNEPYLSTSLQDFWGRRWNILVSNLLRLTVFIPVRSAMIDVAGRRRSAAV 251

Query: 241 -VFVVFVVSGLMHELLFYYIIRVVPTWEVTWFFIIHGVCVAMEIELKSALGKKWQFHWAV 300
            VF VFVVSGLMHELLFYY+ R  P+WEVT FF++ GVCV +E +LK A+G+K++ H+AV
Sbjct: 252 GVFAVFVVSGLMHELLFYYVNRESPSWEVTGFFVLQGVCVLLEFQLKMAVGEKFRMHYAV 305

Query: 301 TGPLTMTFIAITA 308
              LTM F+ +TA
Sbjct: 312 CWVLTMGFVVVTA 305

BLAST of Lsi02G018730 vs. TrEMBL
Match: A5BHD6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_19s0090g01400 PE=4 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 7.6e-72
Identity = 151/313 (48.24%), Postives = 205/313 (65.50%), Query Frame = 1

Query: 1   MRVLLICMASLLYCYSIASKLPKGKLRLLSLLPIFSLFAVLPLDLSYVVLTSGITIFVTW 60
           ++V L  +ASL Y Y I SK+PKGK RLLSLLPIFSLF  LPL LS  +L+     F+TW
Sbjct: 9   IKVCLSVLASLCYSYFIVSKIPKGKFRLLSLLPIFSLFVALPLFLSTAILSGITAFFITW 68

Query: 61  LTTFKLLQFSFDLGPLASNPPLSFPLFASIAFLPTRIKDIKTTPYVQKHRKDSNAPQELD 120
           L TF+L  FSFDLGPL++  P S  +F +IA LP +IK  +  P  Q+  K         
Sbjct: 69  LATFRLALFSFDLGPLSTGSPKSLLVFIAIACLPIKIKPNQQHPSRQEPHK--------- 128

Query: 121 QEPPPKLPLNLPAKVLLFALLVLGKSHVDLVHPIVKLCLNCGILYFYLDMVMSLSNVFVR 180
              PP+LPLN   KVL F + +    + +LVHP + L L C  ++ +L+++ SL +  VR
Sbjct: 129 ---PPRLPLNFAVKVLAFGVFIGFYQYKELVHPKIFLGLLCCQVFLFLEVLFSLCSALVR 188

Query: 181 SSFGVEVRRPFDEPYLATSLQNFWGRRWNRLVSETLHNTIYKPVR---YDVGSPRWM--- 240
            + G+EV +P DEPYL+TSLQ+FWGRRWN +V+  L +T+YKPV+     V S RW    
Sbjct: 189 CTMGLEVEQPSDEPYLSTSLQDFWGRRWNLMVTNLLRHTVYKPVKSAAETVMSERWSPLP 248

Query: 241 AVFVVFVVSGLMHELLFYYIIRVVPTWEVTWFFIIHGVCVAMEIELKSALGKKWQFHWAV 300
           AV   F+VSGLMHELLFYY+ RV P+WE+T FF++HGVC+ +E+ +KS    +W+ HWA 
Sbjct: 249 AVVATFLVSGLMHELLFYYVNRVSPSWEMTSFFVLHGVCLVVEVGVKSVFSGRWRLHWAA 308

Query: 301 TGPLTMTFIAITA 308
           + PLT+ F+  T+
Sbjct: 309 SVPLTVGFVVATS 309

BLAST of Lsi02G018730 vs. TAIR10
Match: AT5G55380.1 (AT5G55380.1 MBOAT (membrane bound O-acyl transferase) family protein)

HSP 1 Score: 187.2 bits (474), Expect = 1.5e-47
Identity = 125/314 (39.81%), Postives = 175/314 (55.73%), Query Frame = 1

Query: 1   MRVLLICMASLLYCYSIASKLPKGKLRLLSLLPIFSLFAVLPLDLSYVVLTSGITIFVTW 60
           + V +  + SL YCY I+SKL KG LRLLS+LP+  LF VLPL LS V   +   +F++W
Sbjct: 9   IEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCAISVLFLSW 68

Query: 61  LTTFKLLQFSFDLGPLASNPPLSFPLFASIAFLPTRIKDIKTTPYVQKHRKDSNAPQELD 120
           L  FKLL F+FD GPL   PP     F   A LP +I+   +           NA   L 
Sbjct: 69  LANFKLLLFAFDEGPLFPLPP-KLSRFICFACLPIKIRQDPSP----------NAIPNLH 128

Query: 121 QEPPPKLPLNLPAKVLLFALLVLGKSHVDLVHPIVKLCLNCGILYFYLDMVMSLSNVFVR 180
            +P PK  L +  K+L+  +L+    + D +   V L L C  +Y  +++V+      V 
Sbjct: 129 PKPMPKWVLAV--KILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVVS 188

Query: 181 SSFGVEVRRPFDEPYLATSLQNFWGRRWNRLVSETLHNTIYKPVR------YDVGSPRWM 240
           +  G  +   F+EPYLATSLQ+FW RRWN +VS  L +T++ PV+             + 
Sbjct: 189 TLLGCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFA 248

Query: 241 AVFVVFVVSGLMHELLFYYIIRVVPTWEVTWFFIIHGVCVAMEIELKSALGKKW--QFHW 300
            V   F VSGLMHELL++Y+IR  PTWEVT FF++HG   A EI +K     +W    H 
Sbjct: 249 GVMASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRT---QWLRPPHR 306

Query: 301 AVTGPLTMTFIAIT 307
           AV+G + +TF+++T
Sbjct: 309 AVSGLVVLTFVSVT 306

BLAST of Lsi02G018730 vs. TAIR10
Match: AT3G51970.1 (AT3G51970.1 acyl-CoA sterol acyl transferase 1)

HSP 1 Score: 185.3 bits (469), Expect = 5.8e-47
Identity = 111/309 (35.92%), Postives = 175/309 (56.63%), Query Frame = 1

Query: 6   ICMASLLYCYSIASKLPKGKLRLLSLLPIFSLFAVLPLDLSYVVLTSGIT-IFVTWLTTF 65
           + + SL Y + IA  +PKG  RL+   P+F +F ++P  L Y +   GIT  F+ WL  F
Sbjct: 10  LVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPF-LIYSLHLLGITAFFIAWLANF 69

Query: 66  KLLQFSFDLGPLASN-PPLSFPLFASIAFLPTRIKDIKTTPYVQKHRKDSNAPQELDQEP 125
           KLL F+   GPL+SN  PLS P+F +++ LP +I+          H   +  P       
Sbjct: 70  KLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGP------- 129

Query: 126 PPKLPLNLPAKVLLFALLVLGKSHVDLVHPIVKLCLNCGILYFYLDMVMSLSNVFVRSSF 185
                L    K +   L++    +   +   V L L    +YF L+++++ +   VR+  
Sbjct: 130 -----LIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMS 189

Query: 186 GVEVRRPFDEPYLATSLQNFWGRRWNRLVSETLHNTIYKPVR--YDVGSPRW---MAVFV 245
            +E+   F++PYLATSLQ+FWGRRWN +V+  L  T+Y+P    + V  P +   +A F 
Sbjct: 190 DLELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFSVLGPNYSQILAAFG 249

Query: 246 VFVVSGLMHELLFYYIIRVVPTWEVTWFFIIHGVCVAMEIELKSALGKKWQFHWAVTGPL 305
            FVVSG+MHEL+F+Y+ R+ P W++ WFF+I+G C  +EI +K  +  +W+F  A++  L
Sbjct: 250 TFVVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVL 305

Query: 306 TMTFIAITA 308
           T+TF+ +TA
Sbjct: 310 TLTFVMVTA 305

BLAST of Lsi02G018730 vs. TAIR10
Match: AT1G34490.1 (AT1G34490.1 MBOAT (membrane bound O-acyl transferase) family protein)

HSP 1 Score: 176.4 bits (446), Expect = 2.7e-44
Identity = 120/303 (39.60%), Postives = 170/303 (56.11%), Query Frame = 1

Query: 10  SLLYCYSIASKLPKGKLRLLSLLPIFSLFAVLPLDLSYVVLTSGITIFVTWLTTFKLLQF 69
           S+ YCY I SK+ +G  RLLS+LP+  LF VLPL   + + +S     ++ L  FKL+ F
Sbjct: 18  SVSYCYYIPSKIKRGVHRLLSVLPVCVLFLVLPLFFVFTIFSSTTAFCLSILANFKLILF 77

Query: 70  SFDLGPLASNPPLSFPLFASIAFLPTRIKDIKTTPYVQKHRKDSNAPQELDQEPPPKLPL 129
           +FD GPL    PL   LF  I F    IK ++T P  Q H               PK  L
Sbjct: 78  AFDKGPLL---PLPTNLFRFICFTCLPIK-LQTKPNSQNHL--------------PKWVL 137

Query: 130 NLPAKVLLFALLVLGKSHVDLVHPIVKLCLNCGILYFYLDMVMSLSNVFVRSSFGVEVRR 189
             P+KV +F LL+  +S+  L+ PI+ L L    LY  LD+++++ N  +      ++  
Sbjct: 138 --PSKVAIFVLLLNIRSYKILLPPILLLGLYPLHLYIVLDVLLTIVNALLTIILRCDLEP 197

Query: 190 PFDEPYLATSLQNFWGRRWNRLVSETLHNTIYKPVR----YDVGS--PRWMAVFVVFVVS 249
            F+EPYLATSLQ+FWG RWN +VS      +Y PVR    + + S   R+M     F VS
Sbjct: 198 HFNEPYLATSLQDFWGHRWNLMVSAIYRPGVYSPVRSVCQHQMRSDWARFMGCMTTFFVS 257

Query: 250 GLMHELLFYYIIRVVPTWEVTWFFIIHGVCVAMEIELKSALGKKWQFHWAVTGPLTMTFI 307
           GL+HEL+++YI R  PT EVTWFF++HGVC AMEI +K  +  +W     +   +T+ F+
Sbjct: 258 GLIHELVYFYINREKPTLEVTWFFVLHGVCTAMEIAVKRKM--QWSLSPMLLRLITVGFL 298

BLAST of Lsi02G018730 vs. TAIR10
Match: AT5G55340.1 (AT5G55340.1 MBOAT (membrane bound O-acyl transferase) family protein)

HSP 1 Score: 173.7 bits (439), Expect = 1.7e-43
Identity = 112/314 (35.67%), Postives = 173/314 (55.10%), Query Frame = 1

Query: 1   MRVLLICMASLLYCYSIASKLPKGKLRLLSLLPIFSLFAVLPLDLSYVVLTSGITIFVTW 60
           ++V +  + S+ YCY I  ++  G  R LS+ P+ +LF VLPL  S + L+     F+TW
Sbjct: 9   IKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSLITAFFLTW 68

Query: 61  LTTFKLLQFSFDLGPLASNPPLSFPLFASIAFLPTRIKDIKTTPYVQKHRKDSNAPQELD 120
           L  FKL+ FSFD GPL   P  +FP F      P +++     P  Q H           
Sbjct: 69  LANFKLILFSFDKGPLIPIPT-NFPRFFCFTCFPIKVQQ---NPKSQNHL---------- 128

Query: 121 QEPPPKLPLNLPAKVLLFALLVLGKSHVDLVHPIVKLCLNCGILYFYLDMVMSLSNVFVR 180
               PKL   +  K+ +FA+L+   S+   + P + L L    LY  ++++++   V V 
Sbjct: 129 ----PKLVFAI--KLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVVF 188

Query: 181 SSFGVEVRRPFDEPYLATSLQNFWGRRWNRLVSETLHNTIYKPVR------YDVGSPRWM 240
            S G ++    ++PYLATSLQ+FWGRRWN +V   L   +Y P+R         G   + 
Sbjct: 189 ISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALFP 248

Query: 241 AVFVVFVVSGLMHELLFYYIIRVVPTWEVTWFFIIHGVCVAME--IELKSALGKKWQFHW 300
            +   F+VSGL+HELLF+Y+IR +PT EVT FF++HGVC A+E  ++ K+ + ++W+   
Sbjct: 249 GILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLSP 302

Query: 301 AVTGPLTMTFIAIT 307
            V+  LT+ F+ +T
Sbjct: 309 GVSRVLTVGFVFVT 302

BLAST of Lsi02G018730 vs. TAIR10
Match: AT5G55360.1 (AT5G55360.1 MBOAT (membrane bound O-acyl transferase) family protein)

HSP 1 Score: 173.3 bits (438), Expect = 2.3e-43
Identity = 109/316 (34.49%), Postives = 173/316 (54.75%), Query Frame = 1

Query: 1   MRVLLICMASLLYCYSIASKLPKGKLRLLSLLPIFSLFAVLPLDLSYVVLTSGITIFVTW 60
           +++ +  + S+ YCY +++ +  G  RLLS+LP+ +LF V PL  SYV  +  +  F++W
Sbjct: 9   IKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSGCMAFFLSW 68

Query: 61  LTTFKLLQFSFDLGPLASNPPLSFPLFASIAFLPTRIKDIKTTPYVQKHRKDSNAPQELD 120
           L  FKL+ FSFD GPL S  P +   F  I   P + +     P +Q +           
Sbjct: 69  LANFKLILFSFDQGPL-SPLPRTLSRFICITCFPIKPQQ---NPNIQNY----------- 128

Query: 121 QEPPPKLPLNLPA-KVLLFALLVLGKSHVDLVHPIVKLCLNCGILYFYLDMVMSLSNVFV 180
                K+P+ L A KV++F +L+    +   + P + L  N   ++  L++V  L    V
Sbjct: 129 -----KIPIWLFAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALV 188

Query: 181 RSSFGVEVRRPFDEPYLATSLQNFWGRRWNRLVSETLHNTIYKPVR------YDVGSPRW 240
             + G ++    +EPYLATSLQ+FWGRRWN +V   L   +Y P R       +     +
Sbjct: 189 FITLGCDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMF 248

Query: 241 MAVFVVFVVSGLMHELLFYYIIRVVPTWEVTWFFIIHGVCVAMEIELKSA--LGKKWQFH 300
           + VF  F+VSG +HE+LF+Y+ R VPT EVTWFF++HGVC   E+ +K +  + + W+  
Sbjct: 249 LGVFAAFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVS 304

Query: 301 WAVTGPLTMTFIAITA 308
             V+  LT+ F+ +T+
Sbjct: 309 PTVSRLLTVGFVVVTS 304

BLAST of Lsi02G018730 vs. NCBI nr
Match: gi|449466618|ref|XP_004151023.1| (PREDICTED: probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis sativus])

HSP 1 Score: 557.8 bits (1436), Expect = 1.2e-155
Identity = 276/308 (89.61%), Postives = 293/308 (95.13%), Query Frame = 1

Query: 1   MRVLLICMASLLYCYSIASKLPKGKLRLLSLLPIFSLFAVLPLDLSYVVLTSGITIFVTW 60
           M+VLLICMASL+YCY IASKLPKGK RLLSLLPIFSLFAVLPLDLSYVVLTSGITIFVTW
Sbjct: 11  MKVLLICMASLMYCYYIASKLPKGKPRLLSLLPIFSLFAVLPLDLSYVVLTSGITIFVTW 70

Query: 61  LTTFKLLQFSFDLGPLASNPPLSFPLFASIAFLPTRIKDIKTTPYVQKHRKDSNAPQELD 120
           LTTFKL+QFSFDLGPLAS+PPLSFPLFASIAFLPTRIKDIKTTPYVQ H+KDSNAPQELD
Sbjct: 71  LTTFKLIQFSFDLGPLASDPPLSFPLFASIAFLPTRIKDIKTTPYVQNHKKDSNAPQELD 130

Query: 121 QEPPPKLPLNLPAKVLLFALLVLGKSHVDLVHPIVKLCLNCGILYFYLDMVMSLSNVFVR 180
           QE PPKLPLNLPAK  +FALLV+ K+HV LVHPI+KL LNCGILYFYLDM+MS+SN FVR
Sbjct: 131 QETPPKLPLNLPAKAFIFALLVICKNHVHLVHPILKLILNCGILYFYLDMIMSISNEFVR 190

Query: 181 SSFGVEVRRPFDEPYLATSLQNFWGRRWNRLVSETLHNTIYKPVRYDVGSPRWMAVFVVF 240
            SFG+EVRR FDEPYLATSLQNFWGRRWNRLVSETLHN IYKP+RYDVGSPRWMAVFVVF
Sbjct: 191 LSFGIEVRRSFDEPYLATSLQNFWGRRWNRLVSETLHNMIYKPIRYDVGSPRWMAVFVVF 250

Query: 241 VVSGLMHELLFYYIIRVVPTWEVTWFFIIHGVCVAMEIELKSALGKKWQFHWAVTGPLTM 300
           VVSGLMHELLFYYIIRVVPTWEVTWFF+IHGV VA+EIELKSALGKKWQFHWAVTGPLTM
Sbjct: 251 VVSGLMHELLFYYIIRVVPTWEVTWFFVIHGVFVALEIELKSALGKKWQFHWAVTGPLTM 310

Query: 301 TFIAITAH 309
            F+A+TAH
Sbjct: 311 AFMAVTAH 318

BLAST of Lsi02G018730 vs. NCBI nr
Match: gi|659093758|ref|XP_008447703.1| (PREDICTED: probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo])

HSP 1 Score: 543.9 bits (1400), Expect = 1.8e-151
Identity = 267/308 (86.69%), Postives = 291/308 (94.48%), Query Frame = 1

Query: 1   MRVLLICMASLLYCYSIASKLPKGKLRLLSLLPIFSLFAVLPLDLSYVVLTSGITIFVTW 60
           ++VLL+CM SLLYCY IASKLPKGK RLLSLLPIFSLFAVLPLDLSYVVLTSGITIFVTW
Sbjct: 11  IKVLLVCMVSLLYCYYIASKLPKGKPRLLSLLPIFSLFAVLPLDLSYVVLTSGITIFVTW 70

Query: 61  LTTFKLLQFSFDLGPLASNPPLSFPLFASIAFLPTRIKDIKTTPYVQKHRKDSNAPQELD 120
           LTTFKL+QFSFD+GPLAS+PPLSFPLFASIAFLPTR++DIKTTPYVQ H+KDS APQ+LD
Sbjct: 71  LTTFKLIQFSFDVGPLASDPPLSFPLFASIAFLPTRLRDIKTTPYVQNHKKDSIAPQKLD 130

Query: 121 QEPPPKLPLNLPAKVLLFALLVLGKSHVDLVHPIVKLCLNCGILYFYLDMVMSLSNVFVR 180
           QEPPPKLPLNLPAK  LFALLV+ KSHV LVHP++ L  NCGILYFYLDM+MS+SN FVR
Sbjct: 131 QEPPPKLPLNLPAKAFLFALLVVCKSHVHLVHPVLNLIFNCGILYFYLDMIMSISNDFVR 190

Query: 181 SSFGVEVRRPFDEPYLATSLQNFWGRRWNRLVSETLHNTIYKPVRYDVGSPRWMAVFVVF 240
            SFGV+VRR FDEPYLATSLQNFWGRRWNRLVSETLHNTIYKP+RY+VGSPRWMAVF+VF
Sbjct: 191 LSFGVKVRRSFDEPYLATSLQNFWGRRWNRLVSETLHNTIYKPIRYNVGSPRWMAVFMVF 250

Query: 241 VVSGLMHELLFYYIIRVVPTWEVTWFFIIHGVCVAMEIELKSALGKKWQFHWAVTGPLTM 300
           VVSGLMHELLFYYIIRVVPTWEVTWFF+IHGV VA+EIELKSALGKKW+FHWAVTGPLTM
Sbjct: 251 VVSGLMHELLFYYIIRVVPTWEVTWFFVIHGVFVALEIELKSALGKKWKFHWAVTGPLTM 310

Query: 301 TFIAITAH 309
            F+A+TAH
Sbjct: 311 AFMAVTAH 318

BLAST of Lsi02G018730 vs. NCBI nr
Match: gi|659093760|ref|XP_008447704.1| (PREDICTED: probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo])

HSP 1 Score: 382.5 bits (981), Expect = 7.0e-103
Identity = 200/306 (65.36%), Postives = 235/306 (76.80%), Query Frame = 1

Query: 3   VLLICMASLLYCYSIASKLPKGKLRLLSLLPIFSLFAVLPLDLSYVVLTSGITIFVTWLT 62
           VL I + S LYCY +ASK  K KLRLLSLLP+FS+F VLPL  S ++ TS + +FVTWLT
Sbjct: 11  VLPIFILSSLYCYFVASKFSKAKLRLLSLLPVFSIFLVLPLYFSSILFTSSVGLFVTWLT 70

Query: 63  TFKLLQFSFDLGPLASNPPLSFPLFASIAFLPTRIKDIKTTPYVQKHRKDSNAPQELDQE 122
           TFKLL  +FD GPLASNPPLSFPLFASIAF PTR K  KTTP+              DQE
Sbjct: 71  TFKLLLVTFDTGPLASNPPLSFPLFASIAFFPTRTKHNKTTPH--------------DQE 130

Query: 123 PPPKL-PLNLPAKVLLFALLVLGKSHVDLVHP-IVKLCLNCGILYFYLDMVMSLSNVFVR 182
             PKL  L+LP KV++ A+L+LG S++DLV P  VK  +NC  +YFYLD+VMSLSNVFVR
Sbjct: 131 SLPKLLHLDLPTKVVILAILILGNSYIDLVCPNYVKSWINCATMYFYLDVVMSLSNVFVR 190

Query: 183 SSFGVEVRRPFDEPYLATSLQNFWGRRWNRLVSETLHNTIYKPVRYDVGSPRWMAVFVVF 242
           S+FGVEVR+PF+EPYLATSLQN WGRRWN +VSETLH+TIYKP+RY VG PRW+AV  VF
Sbjct: 191 STFGVEVRQPFNEPYLATSLQNLWGRRWNLIVSETLHSTIYKPIRYKVGMPRWVAVVTVF 250

Query: 243 VVSGLMHELLFYYIIRVVPTWEVTWFFIIHGVCVAMEIELKSALGKKWQFHWAVTGPLTM 302
           VVSGLMHELL+YYI+R +PTWE  WFF+IHGVC+A+EIELK A  K+WQ   A +  LT 
Sbjct: 251 VVSGLMHELLYYYIVRAIPTWEFMWFFVIHGVCLALEIELKKAYEKRWQLQLAASTLLTT 302

Query: 303 TFIAIT 307
           TF A T
Sbjct: 311 TFTATT 302

BLAST of Lsi02G018730 vs. NCBI nr
Match: gi|449444620|ref|XP_004140072.1| (PREDICTED: probable long-chain-alcohol O-fatty-acyltransferase 6 [Cucumis sativus])

HSP 1 Score: 380.9 bits (977), Expect = 2.0e-102
Identity = 200/307 (65.15%), Postives = 235/307 (76.55%), Query Frame = 1

Query: 1   MRVLLICMASLLYCYSIASKLPKGKLRLLSLLPIFSLFAVLPLDLSYVVLTSGITIFVTW 60
           ++VL + + S LYCY IASK  K KLRLLSLLP+FS+F VLPL  S ++ TS + +FVTW
Sbjct: 9   IKVLPVFILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTSSVGLFVTW 68

Query: 61  LTTFKLLQFSFDLGPLASNPPLSFPLFASIAFLPTRIKDIKTTPYVQKHRKDSNAPQELD 120
           LTTFKLL F+FD GPLASNPPLSFPLFASIAF PTR K  KTTP+ QK            
Sbjct: 69  LTTFKLLLFTFDSGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQKSL---------- 128

Query: 121 QEPPPKLPLNLPAKVLLFALLVLGKSHVDLVHPI-VKLCLNCGILYFYLDMVMSLSNVFV 180
              P  LPLNLP KV++ A+L+LG S++DLV P  VK  +N   +YFYLD+VMSLSNVFV
Sbjct: 129 ---PKLLPLNLPTKVVILAILILGNSYIDLVCPNNVKSWINGATVYFYLDVVMSLSNVFV 188

Query: 181 RSSFGVEVRRPFDEPYLATSLQNFWGRRWNRLVSETLHNTIYKPVRYDVGSPRWMAVFVV 240
           +S+FGVEVR+PF+EPYLATSLQN WGRRWN LVSETLH+TIYKP+RY VG PRW+AV  V
Sbjct: 189 QSTFGVEVRQPFNEPYLATSLQNLWGRRWNLLVSETLHSTIYKPIRYKVGMPRWVAVVTV 248

Query: 241 FVVSGLMHELLFYYIIRVVPTWEVTWFFIIHGVCVAMEIELKSALGKKWQFHWAVTGPLT 300
           FVVSGLMHELL+YY++R  PTWE TWFF+IHGVC+A+EIELK A  KKWQ   AV+  LT
Sbjct: 249 FVVSGLMHELLYYYMVRANPTWEFTWFFVIHGVCLALEIELKRAYEKKWQLQRAVSTLLT 302

Query: 301 MTFIAIT 307
             F   T
Sbjct: 309 TMFTGTT 302

BLAST of Lsi02G018730 vs. NCBI nr
Match: gi|778725461|ref|XP_004151617.2| (PREDICTED: probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis sativus])

HSP 1 Score: 312.4 bits (799), Expect = 8.9e-82
Identity = 171/313 (54.63%), Postives = 216/313 (69.01%), Query Frame = 1

Query: 2   RVLLICMASLLYCYSIASKLPKGKLRLLSLLPIFSLFAVLPLDLSYVVLTSGITIFVTWL 61
           +V L  M SLLY Y I+SKLPKGK RL+SL P FSLFA+LPL LS V L+  +  F+TWL
Sbjct: 13  KVSLTIMVSLLYSYFISSKLPKGKFRLISLFPTFSLFALLPLSLSSVFLSGTVAFFITWL 72

Query: 62  TTFKLLQFSFDLGPLASNPPLSFPLFASIAFLPTRIKDIKTTPYVQKHRKDSNAPQELDQ 121
           TTFKLL FSF+LGPL S+PPL+FPLF ++A LP +IK  +  P  +   K +N       
Sbjct: 73  TTFKLLLFSFNLGPLVSDPPLTFPLFVTVACLPIKIKPREIDPDYKYLEKYAN------- 132

Query: 122 EPPPKLPLNLPAKVLLFALLVLGKSHVDLVHPIVKLCLNCGILYFYLDMVMSLSNVFVRS 181
              PKL LNLPAK+LLFA+L+    H+D   P VK+C+ C  LY +++ ++ +S+  VRS
Sbjct: 133 ---PKLGLNLPAKILLFAILIAAGDHIDQFRPNVKICVYCVTLYLFVEFLLGVSSAIVRS 192

Query: 182 SF-GVEVRRPFDEPYLATSLQNFWGRRWNRLVSETLHNTIYKPVRYDVG---SPRWMA-- 241
           SF GV +    +EPYLATSLQ+FWGRRWN LVS TL   IY+PVR  VG     RW A  
Sbjct: 193 SFDGVVLEPSSNEPYLATSLQDFWGRRWNLLVSNTLRYGIYRPVRAAVGGVVGKRWAAGT 252

Query: 242 -VFVVFVVSGLMHELLFYYIIRVVPTWEVTWFFIIHGVCVAMEIELKSALGKKWQFHWAV 301
            V  VFVVSG+MHE L YYI R VPTWEVTWFF++ GVCV +E ELK+ +G++WQ   AV
Sbjct: 253 GVMAVFVVSGVMHEQLVYYITREVPTWEVTWFFVLQGVCVVLEFELKTVVGRRWQPPAAV 312

Query: 302 TGPLTMTFIAITA 308
              LT++F+ +TA
Sbjct: 313 AWLLTLSFLVVTA 315

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
WAXS1_ARATH2.7e-4639.81Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana GN... [more]
WAXS1_SIMCH4.6e-4638.85Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis PE=1 SV=1[more]
ASAT1_ARATH1.0e-4535.92Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana GN=ASAT1 PE=1 SV=1[more]
WAXSA_ARATH4.8e-4339.60Putative long-chain-alcohol O-fatty-acyltransferase 10 OS=Arabidopsis thaliana G... [more]
WAXS5_ARATH3.1e-4235.67Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana GN... [more]
Match NameE-valueIdentityDescription
A0A0A0K522_CUCSA8.5e-15689.61Uncharacterized protein OS=Cucumis sativus GN=Csa_7G091730 PE=4 SV=1[more]
A0A0A0K6W1_CUCSA1.4e-10265.15Uncharacterized protein OS=Cucumis sativus GN=Csa_7G091220 PE=4 SV=1[more]
A0A0A0K576_CUCSA6.2e-8254.63Uncharacterized protein OS=Cucumis sativus GN=Csa_7G179120 PE=4 SV=1[more]
A0A0A0K8E9_CUCSA1.8e-7351.76Uncharacterized protein OS=Cucumis sativus GN=Csa_7G145990 PE=4 SV=1[more]
A5BHD6_VITVI7.6e-7248.24Putative uncharacterized protein OS=Vitis vinifera GN=VIT_19s0090g01400 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
AT5G55380.11.5e-4739.81 MBOAT (membrane bound O-acyl transferase) family protein[more]
AT3G51970.15.8e-4735.92 acyl-CoA sterol acyl transferase 1[more]
AT1G34490.12.7e-4439.60 MBOAT (membrane bound O-acyl transferase) family protein[more]
AT5G55340.11.7e-4335.67 MBOAT (membrane bound O-acyl transferase) family protein[more]
AT5G55360.12.3e-4334.49 MBOAT (membrane bound O-acyl transferase) family protein[more]
Match NameE-valueIdentityDescription
gi|449466618|ref|XP_004151023.1|1.2e-15589.61PREDICTED: probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis sativu... [more]
gi|659093758|ref|XP_008447703.1|1.8e-15186.69PREDICTED: probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo][more]
gi|659093760|ref|XP_008447704.1|7.0e-10365.36PREDICTED: probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo][more]
gi|449444620|ref|XP_004140072.1|2.0e-10265.15PREDICTED: probable long-chain-alcohol O-fatty-acyltransferase 6 [Cucumis sativu... [more]
gi|778725461|ref|XP_004151617.2|8.9e-8254.63PREDICTED: probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis sativu... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016747 transferase activity, transferring acyl groups other than amino-acyl groups

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G018730.1Lsi02G018730.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR31595FAMILY NOT NAMEDcoord: 1..307
score: 1.8E
NoneNo IPR availablePANTHERPTHR31595:SF8SUBFAMILY NOT NAMEDcoord: 1..307
score: 1.8E