Lsi02G015340 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi02G015340
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Matrix metalloproteinase
Location	chr02 : 21203172 .. 21204062 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCTAAATTATGTCTCCAACTCTTCCTACTTCTTGCCTTCATTACACCTCACTTGACCTCAACCCGCCCTCTTCACAACCACAAATCATCTCGACTTCTATTTCCCAAGCATCTTCAAGGAAGTCGTAAAGACGATAACATCGAAGGAATCCATAAGGTCAAGGCTTACCTTCAACGTTATGGTTACTTAAGCAATAAAAGCGAAAACAAATTGAATACAGACTCATTCGACGAGGACTTGGAATCCGCCATTAAATCATACCAAACGTTCTTCAAGCTTAATGTGAGTGGAGTCTTAGACAAGGAGACCCTACAACAAATGTCGAAGCCTCGATGTGGGGTGACAGACAATTTCATAGTATCACAAGGTAAGAATGTGGAAATGAATGGTTCTCATTATTCATTCTTCCCAAAGAAACGAACATGGCCAGTTGAGAAGAAGCATTTGACGTACGGATTTATCCACAATTACCCACCAGAGCACGTGGCTGCAGTGATGCGAGCTTTAGAGACATGGGCAGATAATACGAAATTCACCTTTGCCCAGGCTGCAAGGGTACAATCGGCAGACATTTTGTTAAGCTTTGAGAGAGGAGACCATGGAGATGGGCGTCCGTTTGATGGAGAAGGGGGAGTATTGGCACATGCATTTGGACCAATAGATGGGAGAATACATTTTGATGCGGATGAGCCATGGGAAAGAGGTCCTATTTTGGAACAGTTTGATGTGGAGTCGGTGGCATTGCATGAGTTGGGACATGCACTTGGGCTTGGCCATAGCAACATTGAATCCGCTGTCATGTGGCCTGATATGGACTCTGGTGTCACCAAGACCCGATTGACTATTGACGATATTGAAGGCATCCGTGCTTTATATGGCCGTTAA

mRNA sequence

ATGGCTTCTAAATTATGTCTCCAACTCTTCCTACTTCTTGCCTTCATTACACCTCACTTGACCTCAACCCGCCCTCTTCACAACCACAAATCATCTCGACTTCTATTTCCCAAGCATCTTCAAGGAAGTCGTAAAGACGATAACATCGAAGGAATCCATAAGGTCAAGGCTTACCTTCAACGTTATGGTTACTTAAGCAATAAAAGCGAAAACAAATTGAATACAGACTCATTCGACGAGGACTTGGAATCCGCCATTAAATCATACCAAACGTTCTTCAAGCTTAATGTGAGTGGAGTCTTAGACAAGGAGACCCTACAACAAATGTCGAAGCCTCGATGTGGGGTGACAGACAATTTCATAGTATCACAAGGTAAGAATGTGGAAATGAATGGTTCTCATTATTCATTCTTCCCAAAGAAACGAACATGGCCAGTTGAGAAGAAGCATTTGACGTACGGATTTATCCACAATTACCCACCAGAGCACGTGGCTGCAGTGATGCGAGCTTTAGAGACATGGGCAGATAATACGAAATTCACCTTTGCCCAGGCTGCAAGGGTACAATCGGCAGACATTTTGTTAAGCTTTGAGAGAGGAGACCATGGAGATGGGCGTCCGTTTGATGGAGAAGGGGGAGTATTGGCACATGCATTTGGACCAATAGATGGGAGAATACATTTTGATGCGGATGAGCCATGGGAAAGAGGTCCTATTTTGGAACAGTTTGATGTGGAGTCGGTGGCATTGCATGAGTTGGGACATGCACTTGGGCTTGGCCATAGCAACATTGAATCCGCTGTCATGTGGCCTGATATGGACTCTGGTGTCACCAAGACCCGATTGACTATTGACGATATTGAAGGCATCCGTGCTTTATATGGCCGTTAA

Coding sequence (CDS)

ATGGCTTCTAAATTATGTCTCCAACTCTTCCTACTTCTTGCCTTCATTACACCTCACTTGACCTCAACCCGCCCTCTTCACAACCACAAATCATCTCGACTTCTATTTCCCAAGCATCTTCAAGGAAGTCGTAAAGACGATAACATCGAAGGAATCCATAAGGTCAAGGCTTACCTTCAACGTTATGGTTACTTAAGCAATAAAAGCGAAAACAAATTGAATACAGACTCATTCGACGAGGACTTGGAATCCGCCATTAAATCATACCAAACGTTCTTCAAGCTTAATGTGAGTGGAGTCTTAGACAAGGAGACCCTACAACAAATGTCGAAGCCTCGATGTGGGGTGACAGACAATTTCATAGTATCACAAGGTAAGAATGTGGAAATGAATGGTTCTCATTATTCATTCTTCCCAAAGAAACGAACATGGCCAGTTGAGAAGAAGCATTTGACGTACGGATTTATCCACAATTACCCACCAGAGCACGTGGCTGCAGTGATGCGAGCTTTAGAGACATGGGCAGATAATACGAAATTCACCTTTGCCCAGGCTGCAAGGGTACAATCGGCAGACATTTTGTTAAGCTTTGAGAGAGGAGACCATGGAGATGGGCGTCCGTTTGATGGAGAAGGGGGAGTATTGGCACATGCATTTGGACCAATAGATGGGAGAATACATTTTGATGCGGATGAGCCATGGGAAAGAGGTCCTATTTTGGAACAGTTTGATGTGGAGTCGGTGGCATTGCATGAGTTGGGACATGCACTTGGGCTTGGCCATAGCAACATTGAATCCGCTGTCATGTGGCCTGATATGGACTCTGGTGTCACCAAGACCCGATTGACTATTGACGATATTGAAGGCATCCGTGCTTTATATGGCCGTTAA

Protein sequence

MASKLCLQLFLLLAFITPHLTSTRPLHNHKSSRLLFPKHLQGSRKDDNIEGIHKVKAYLQRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQQMSKPRCGVTDNFIVSQGKNVEMNGSHYSFFPKKRTWPVEKKHLTYGFIHNYPPEHVAAVMRALETWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPIDGRIHFDADEPWERGPILEQFDVESVALHELGHALGLGHSNIESAVMWPDMDSGVTKTRLTIDDIEGIRALYGR

Homology

[Close]

BLAST of Lsi02G015340 vs. Swiss-Prot
Match: 5MMP_ARATH (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 5.2e-55
Identity = 116/270 (42.96%), Postives = 151/270 (55.93%), Query Frame = 1


Query: 40  LQGSRKDDNIEGIHKVKAYLQRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSG 99
           L G    +NI G+ K+K Y +R+GY++        TD FD+ L+SAI +YQ  F L V+G
Sbjct: 48  LAGCHIGENINGLSKLKQYFRRFGYITTTGNC---TDDFDDVLQSAINTYQKNFNLKVTG 107

Query: 100 VLDKETLQQMSKPRCG---VTDNFIVSQGKNVEMNGSHYSFFPKKRTWPVEKKHLTYGFI 159
            LD  TL+Q+ KPRCG   + D      G  +      YSFFP K  WP  K+ LTY F 
Sbjct: 108 KLDSSTLRQIVKPRCGNPDLIDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRDLTYAFA 167

Query: 160 --HNYPPEHVAAVMRALETWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGV 219
             +N   E      RA   WA+ T   F ++  +  ADI++ F  G+HGDG PFDG  G 
Sbjct: 168 PQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGT 227

Query: 220 LAHAFGPIDGRIHFDADEPW--ERG-------PILEQFDVESVALHELGHALGLGHSNIE 279
           LAHA  P  G +H D DE W    G       P+    D+ESVA+HE+GH LGLGHS++E
Sbjct: 228 LAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVE 287

Query: 280 SAVMWPDMDSGVTKTRLTIDDIEGIRALYG 296
            A+M+P +  G  K  L  DDIEGI+ LYG
Sbjct: 288 DAIMFPAISGGDRKVELAKDDIEGIQHLYG 314

[Close]

BLAST of Lsi02G015340 vs. Swiss-Prot
Match: 2MMP_ARATH (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 3.7e-53
Identity = 126/319 (39.50%), Postives = 179/319 (56.11%), Query Frame = 1


Query: 7   LQLFLLL----AFITPHLTSTRP-LHNHKSSRLLFP--KHLQGSRKDDNIEGIHKVKAYL 66
           L LFL++    A+  P+ T+  P L N  ++R+ +    +  G     N++G++++K Y 
Sbjct: 9   LSLFLIVSPASAWFFPNSTAVPPSLRN--TTRVFWDAFSNFTGCHHGQNVDGLYRIKKYF 68

Query: 67  QRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQQMSKPRCG---V 126
           QR+GY+         TD FD+ L++A++ YQT F LNV+G LD  T+Q +  PRCG   V
Sbjct: 69  QRFGYIPETFSGNF-TDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNPDV 128

Query: 127 TDNFIVSQG---KNVEMNGSH--------YSFFPKKRTWPVEKKHLTYGFIHNYP-PEHV 186
            +   +  G   K  E+N S         Y+ FP +  WP  ++ LTY F    P  E V
Sbjct: 129 VNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEV 188

Query: 187 AAVM-RALETWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPID 246
            +V  RA   W+D T   F  +    ++DI + F  GDHGDG PFDG  G LAHAF P  
Sbjct: 189 KSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPS 248

Query: 247 GRIHFDADEPWERGPILEQF-------DVESVALHELGHALGLGHSNIESAVMWPDMDSG 296
           G+ H DADE W     L+ F       D+ESVA+HE+GH LGLGHS++E ++M+P + +G
Sbjct: 249 GKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTG 308

[Close]

BLAST of Lsi02G015340 vs. Swiss-Prot
Match: 3MMP_ARATH (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.0e-50
Identity = 111/279 (39.78%), Postives = 160/279 (57.35%), Query Frame = 1


Query: 42  GSRKDDNIEGIHKVKAYLQRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVL 101
           G       +G++ +K Y Q +GY++  + +   TD FD+ L++A++ YQ  F+LNV+GVL
Sbjct: 51  GCHAGKKYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVL 110

Query: 102 DKETLQQMSKPRCGVTD-----NFIVSQGKNVEMNGS----------HYSFFPKKRTWPV 161
           D+ TL+ +  PRCG  D     + + S  K  E++ +          HYSFFP +  WP 
Sbjct: 111 DELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPR 170

Query: 162 EKKHLTYGFI-HNYPPEHVAAVM-RALETWADNTKFTFAQAARVQSADILLSFERGDHGD 221
            ++ LTY F   N   E V +V  RA   W + T  TF +  R  ++DI + F  G+HGD
Sbjct: 171 NRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGD 230

Query: 222 GRPFDGEGGVLAHAFGPIDGRIHFDADEPW----ERGP----ILEQFDVESVALHELGHA 281
           G PFDG    LAHAF P  G  H D +E W    E G     + E  D+ESVA+HE+GH 
Sbjct: 231 GEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHL 290

Query: 282 LGLGHSNIESAVMWPDMDSGVTKTRLTIDDIEGIRALYG 296
           LGLGHS++E ++M+P + +G  K  LT DD+EG++ LYG
Sbjct: 291 LGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYG 329

[Close]

BLAST of Lsi02G015340 vs. Swiss-Prot
Match: MEP1_SOYBN (Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2)

HSP 1 Score: 194.9 bits (494), Expect = 1.2e-48
Identity = 106/257 (41.25%), Postives = 139/257 (54.09%), Query Frame = 1


Query: 48  NIEGIHKVKAYLQRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQ 107
           N +G+  VK Y    GY+ N        D+FD+ L SAIK+YQ  + LNV+G  D  TL+
Sbjct: 50  NYKGLSNVKNYFHHLGYIPNAPHFD---DNFDDTLVSAIKTYQKNYNLNVTGKFDINTLK 109

Query: 108 QMSKPRCGVTDNFIVSQGKNVEMNG-SHYSFFPKKRTWPVEKKHLTYGFIHNYPPEHV-- 167
           Q+  PRCGV D  I++  K       S Y+FF     W      LTY F      +    
Sbjct: 110 QIMTPRCGVPD-IIINTNKTTSFGMISDYTFFKDMPRWQAGTTQLTYAFSPEPRLDDTFK 169

Query: 168 AAVMRALETWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPIDG 227
           +A+ RA   W       F +    ++A+I + F   +HGD  PFDG GG+L HAF P DG
Sbjct: 170 SAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGHAFAPTDG 229

Query: 228 RIHFDADEPW------ERGPILEQFDVESVALHELGHALGLGHSNIESAVMWPDMDSGVT 287
           R HFDADE W       + P+   FD+ESVA+HE+GH LGLGHS+   A+M+P +     
Sbjct: 230 RCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYPSIPPRTR 289

Query: 288 KTRLTIDDIEGIRALYG 296
           K  L  DDI+GIR LYG
Sbjct: 290 KVNLAQDDIDGIRKLYG 302

[Close]

BLAST of Lsi02G015340 vs. Swiss-Prot
Match: 1MMP_ARATH (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.3e-45
Identity = 102/259 (39.38%), Postives = 145/259 (55.98%), Query Frame = 1


Query: 48  NIEGIHKVKAYLQRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQ 107
           ++ G+ ++K YL R+GY+++ SE  + +D FD  LESAI  YQ    L ++G LD  T+ 
Sbjct: 66  HVSGVSELKRYLHRFGYVNDGSE--IFSDVFDGPLESAISLYQENLGLPITGRLDTSTVT 125

Query: 108 QMSKPRCGVTDNFIVSQGKNVEMNGSHYSFFPKKRTWPVEKKHLTYGFIHNYPPEHVAA- 167
            MS PRCGV+D  +      +    +HY++F  K  W   +  LTY     +  +++ + 
Sbjct: 126 LMSLPRCGVSDTHMTINNDFLHTT-AHYTYFNGKPKW--NRDTLTYAISKTHKLDYLTSE 185

Query: 168 -----VMRALETWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGP 227
                  RA   W+     +F +     +AD+ + F  GDHGDG PFDG  G LAHAF P
Sbjct: 186 DVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAP 245

Query: 228 IDGRIHFDADEPW-----ERGPILEQFDVESVALHELGHALGLGHSNIESAVMWPDMDSG 287
            +GR+H DA E W      +G      D+ESVA HE+GH LGLGHS+ ESAVM+P +   
Sbjct: 246 ENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPR 305

Query: 288 VTKTRLTIDDIEGIRALYG 296
             K  LT+DD+ G+  LYG
Sbjct: 306 TKKVDLTVDDVAGVLKLYG 319

[Close]

BLAST of Lsi02G015340 vs. TrEMBL
Match: A0A0A0KS35_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632070 PE=3 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 2.5e-120
Identity = 219/303 (72.28%), Postives = 253/303 (83.50%), Query Frame = 1


Query: 1   MASKLCLQLFLLLAFITPHLTSTRPLHNHKSSRLLFPKHLQGSRKDDNI-EGIHKVKAYL 60
           MA+KLCLQLFLLLAF+TPHLTS RPLHNHKSSRLLFP+HL GS K+D+I EGIHKVKAYL
Sbjct: 1   MAAKLCLQLFLLLAFVTPHLTSARPLHNHKSSRLLFPEHLIGSSKNDSIVEGIHKVKAYL 60

Query: 61  QRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQQMSKPRCGVTDN 120
           QRYGY SN++EN L+TD+FD+DLESAIKSYQ F  L VSGVLD+ETLQQMS+PRCGV DN
Sbjct: 61  QRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVADN 120

Query: 121 F-IVSQ--GKN----VEMNGSHYSFFPKKRTWPVEKKHLTYGFIHNYPPEHVAAVMRALE 180
           F  V+Q  G+N    V++ GSH+ FFP K  WP  K HLTYGF+HNYP +H AAV+RA +
Sbjct: 121 FQSVAQQDGENNNTTVQIGGSHFMFFPGKGKWPYRKWHLTYGFVHNYPMKHAAAVVRAFD 180

Query: 181 TWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPIDGRIHFDADE 240
            WA N+KFTF+ A R+Q+ADILLSFERGDHGDG+PFDGEGG+L HAFGPIDGR+HFDADE
Sbjct: 181 KWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAFGPIDGRVHFDADE 240

Query: 241 PW-ERGPIL-EQFDVESVALHELGHALGLGHSNIESAVMWPDMDSGVTKTRLTIDDIEGI 294
            W E G +  E FD ESVALHE+GHALGLGHS   SAVMW +M++GV KT LTIDDIEG+
Sbjct: 241 QWAEIGSLTNENFDFESVALHEIGHALGLGHSIFPSAVMWANMETGVNKTELTIDDIEGV 300

[Close]

BLAST of Lsi02G015340 vs. TrEMBL
Match: A0A0A0KVC8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632060 PE=3 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 3.5e-114
Identity = 202/303 (66.67%), Postives = 245/303 (80.86%), Query Frame = 1


Query: 1   MASKLCLQLFLLLAFITPHLTSTRPLHNHKSSRLLFPKHLQGSRKDDNI-EGIHKVKAYL 60
           MASKLCLQL LLLAFITPHLTS R LHN K S  LFPKHL GS K+D+I EGIHKVKAYL
Sbjct: 1   MASKLCLQLCLLLAFITPHLTSARHLHNRKPSPFLFPKHLIGSSKNDSIVEGIHKVKAYL 60

Query: 61  QRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQQMSKPRCGVTDN 120
           QRYGYLSN++E KL+TD+FD+DLESAIKSYQ F  L VSGVLD+ETLQQMS+PRCGV D 
Sbjct: 61  QRYGYLSNENEKKLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVRDT 120

Query: 121 FIV-------SQGKNVEMNGSHYSFFPKKRTWPVEKKHLTYGFIHNYPPEHVAAVMRALE 180
           ++        ++  N+E+ GSHY+F+    TWP E++HL+YGFIH++PP+HV  V RA +
Sbjct: 121 YVSVGQQEHENKNTNIEIGGSHYTFYYNHVTWPAERRHLSYGFIHDFPPQHVDTVRRAFQ 180

Query: 181 TWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPIDGRIHFDADE 240
           TW DNTKF+F  + RVQ+ADIL+SFERG+HGD  PFDGEGG+LAH+ G +DGR+HFDAD+
Sbjct: 181 TWEDNTKFSFFLSPRVQTADILVSFERGEHGDHEPFDGEGGILAHSLGAVDGRVHFDADD 240

Query: 241 PWERGPILEQFDVESVALHELGHALGLGHSNIESAVMWPDMDSGVTKTRLTIDDIEGIRA 296
            WE  P +E++D+E++ALHE+GHALGLGHS+   A+MW  M  G  KTRLTIDDIEGIRA
Sbjct: 241 RWEGDP-MERYDLETLALHEIGHALGLGHSSSSLAIMWAYMYKGFPKTRLTIDDIEGIRA 300

[Close]

BLAST of Lsi02G015340 vs. TrEMBL
Match: A0A0A0KU02_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632080 PE=3 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 1.1e-78
Identity = 161/301 (53.49%), Postives = 199/301 (66.11%), Query Frame = 1


Query: 1   MASKLCLQLFLLLAFITPHLTSTRPLHNHKSSRLLFPKHLQGSRKDDNIEGIHKVKAYLQ 60
           MA +  LQLFLL+A I PH+ + R  H H SS      HLQGSR  +NIEGIH VK YL+
Sbjct: 1   MALQFSLQLFLLIASIAPHVITARVRHTHISS------HLQGSRIGNNIEGIHNVKLYLK 60

Query: 61  RYGYLSN-KSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQQMSKPRCGVTD- 120
           RYGYL+N +S N  NT  FD  LESAIK++Q +  LNVSGV+D+ETL  MS PRCG+ D 
Sbjct: 61  RYGYLTNVESTNSNNT--FDVLLESAIKTFQKYHSLNVSGVIDEETLTLMSLPRCGIPDI 120

Query: 121 -NFIVSQGKNVEMNGS----HYSFFPKKRTWPVEKKHLTYGFIHNYPPEHVAAVMRALET 180
            + I     NV+MN S    H++FFP    WPV K +L Y F+ ++P +    VM A+E 
Sbjct: 121 MHNINVMDNNVQMNSSSFHSHFTFFPDNLKWPVSKYNLRYTFLDDFPNDFKEPVMNAMEQ 180

Query: 181 WADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPIDGRIHFDADEP 240
           WA  + F F++A   Q ADI  +F RG+HGDG PFDG+GGVLAHAFGP+DGR+HFD DE 
Sbjct: 181 WALFSLFRFSEAVEAQEADITFNFVRGNHGDGYPFDGKGGVLAHAFGPLDGRVHFDWDED 240

Query: 241 WERGPILEQFDVESVALHELGHALGLGHSNIESAVMWPDMDSGVTKTRLTIDDIEGIRAL 295
           W  G +    +V  VALHELGH LGL HS I  A+MWP M++G     L  DDIEGI+ L
Sbjct: 241 WADGSVGGFINVGMVALHELGHVLGLAHSTIRDAIMWPYMEAGEQTRGLQFDDIEGIQTL 293

[Close]

BLAST of Lsi02G015340 vs. TrEMBL
Match: A0A0A0KNY8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G522910 PE=3 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 8.4e-76
Identity = 152/302 (50.33%), Postives = 196/302 (64.90%), Query Frame = 1


Query: 1   MASKLCLQLFLLLAFITPHLTSTRPLHNHKSSRLLFPKHLQGSRKDDNIEGIHKVKAYLQ 60
           MA K    LFL+L  I  H++S       K + +L P+ L G RK DN+EGIH +K YLQ
Sbjct: 1   MAFKSLQLLFLVLVTIASHVSS-------KHNPILSPQRLYGCRKGDNVEGIHSIKKYLQ 60

Query: 61  RYGYLSNKSEN-----KLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQQMSKPRCG 120
           RYGYLS+ +       +LN++ FD+ LESAIK YQ +  LNVSG+LD+ETL QM KPRCG
Sbjct: 61  RYGYLSHNTSIDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFKPRCG 120

Query: 121 VTDNFIVSQGKNVEMN---GSHYSFFPKKRTWPVEKKHLTYGFIHNYPPEHVAAVMRALE 180
           V D F  +  KN+E +    SHY+ FP    WP  K+HLTY F +N+P + V +V  A+ 
Sbjct: 121 VRDVFKFNSNKNLEDDLEMSSHYTLFPGNLKWPDYKRHLTYVFTNNFPVDFVPSVTEAMA 180

Query: 181 TWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPIDGRIHFDADE 240
            WA  + FTF++A+  QSADI +SF+  DH DG PFDG GGV+ HAF P DGR+H D D+
Sbjct: 181 RWAAQSLFTFSEASDAQSADINISFQIKDHADGLPFDGPGGVVGHAFAPTDGRLHLDGDD 240

Query: 241 PWERGPILEQFDVESVALHELGHALGLGHSNIESAVMWPDMDSGVTKTRLTIDDIEGIRA 295
            W  G  +++ +V +VALHELGH LGL HS +  AVMWP +DS   K  L  DDI G+ A
Sbjct: 241 TWSAGMEVQKVNVMNVALHELGHVLGLAHSTLPQAVMWPYIDSNALK-NLNDDDIAGLHA 294

[Close]

BLAST of Lsi02G015340 vs. TrEMBL
Match: B9RUG6_RICCO (Metalloendoproteinase 1, putative OS=Ricinus communis GN=RCOM_0852690 PE=3 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 2.7e-74
Identity = 152/310 (49.03%), Postives = 190/310 (61.29%), Query Frame = 1


Query: 1   MASKLCLQLFLLLAFITPHLTSTRPLHNH----KSSRLLFPKHLQGSRKDDNIEGIHKVK 60
           MASK      + L  +   L+S    H+H    KSS   F KHLQG  K DN++GIH +K
Sbjct: 1   MASKPFAIFSVTLILLISLLSSAALAHSHSKTEKSSAFDFLKHLQGCHKGDNLKGIHDLK 60

Query: 61  AYLQRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQQMSKPRCGV 120
            YL+ +GYLS K+++  N D FD+ LE A+K+YQ  + LNV+G LD ET+ +M  PRCGV
Sbjct: 61  KYLENFGYLSYKNQSHSNDDDFDDLLEYALKTYQFNYHLNVTGFLDSETVTKMMMPRCGV 120

Query: 121 TDNF-----IVSQGKNVEMNGS-------HYSFFPKKRTWPVEKKHLTYGFIHNYPPEHV 180
            D       + S  KN   + S       HY FFP    WP  K HLTYGF+   P + +
Sbjct: 121 ADIINGTTRMQSSNKNPHHHSSTSFHTVSHYEFFPGNPRWPASKYHLTYGFLPGTPNQAM 180

Query: 181 AAVMRALETWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPIDG 240
             V +A +TWA NT F F +    ++ADI + F RGDHGDG PFDG GG LAHAF P DG
Sbjct: 181 EPVAKAFQTWAANTHFRFTRVQDYRAADITIGFHRGDHGDGSPFDGRGGTLAHAFAPQDG 240

Query: 241 RIHFDADEPWERGPILEQFDVESVALHELGHALGLGHSNIESAVMWPDMDSGVTKTRLTI 295
           R H+D DE W  G     FDVE+VALHE+GH LGLGHS++E A+M P + SG TK  L  
Sbjct: 241 RFHYDGDEHWAVGATQGAFDVETVALHEIGHLLGLGHSSVEGAIMHPSIQSGATK-GLHS 300

[Close]

BLAST of Lsi02G015340 vs. TAIR10
Match: AT1G59970.1 (AT1G59970.1 Matrixin family protein)

HSP 1 Score: 216.1 bits (549), Expect = 2.9e-56
Identity = 116/270 (42.96%), Postives = 151/270 (55.93%), Query Frame = 1


Query: 40  LQGSRKDDNIEGIHKVKAYLQRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSG 99
           L G    +NI G+ K+K Y +R+GY++        TD FD+ L+SAI +YQ  F L V+G
Sbjct: 48  LAGCHIGENINGLSKLKQYFRRFGYITTTGNC---TDDFDDVLQSAINTYQKNFNLKVTG 107

Query: 100 VLDKETLQQMSKPRCG---VTDNFIVSQGKNVEMNGSHYSFFPKKRTWPVEKKHLTYGFI 159
            LD  TL+Q+ KPRCG   + D      G  +      YSFFP K  WP  K+ LTY F 
Sbjct: 108 KLDSSTLRQIVKPRCGNPDLIDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRDLTYAFA 167

Query: 160 --HNYPPEHVAAVMRALETWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGV 219
             +N   E      RA   WA+ T   F ++  +  ADI++ F  G+HGDG PFDG  G 
Sbjct: 168 PQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGT 227

Query: 220 LAHAFGPIDGRIHFDADEPW--ERG-------PILEQFDVESVALHELGHALGLGHSNIE 279
           LAHA  P  G +H D DE W    G       P+    D+ESVA+HE+GH LGLGHS++E
Sbjct: 228 LAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVE 287

Query: 280 SAVMWPDMDSGVTKTRLTIDDIEGIRALYG 296
            A+M+P +  G  K  L  DDIEGI+ LYG
Sbjct: 288 DAIMFPAISGGDRKVELAKDDIEGIQHLYG 314

[Close]

BLAST of Lsi02G015340 vs. TAIR10
Match: AT1G70170.1 (AT1G70170.1 matrix metalloproteinase)

HSP 1 Score: 209.9 bits (533), Expect = 2.1e-54
Identity = 126/319 (39.50%), Postives = 179/319 (56.11%), Query Frame = 1


Query: 7   LQLFLLL----AFITPHLTSTRP-LHNHKSSRLLFP--KHLQGSRKDDNIEGIHKVKAYL 66
           L LFL++    A+  P+ T+  P L N  ++R+ +    +  G     N++G++++K Y 
Sbjct: 9   LSLFLIVSPASAWFFPNSTAVPPSLRN--TTRVFWDAFSNFTGCHHGQNVDGLYRIKKYF 68

Query: 67  QRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQQMSKPRCG---V 126
           QR+GY+         TD FD+ L++A++ YQT F LNV+G LD  T+Q +  PRCG   V
Sbjct: 69  QRFGYIPETFSGNF-TDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNPDV 128

Query: 127 TDNFIVSQG---KNVEMNGSH--------YSFFPKKRTWPVEKKHLTYGFIHNYP-PEHV 186
            +   +  G   K  E+N S         Y+ FP +  WP  ++ LTY F    P  E V
Sbjct: 129 VNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEV 188

Query: 187 AAVM-RALETWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPID 246
            +V  RA   W+D T   F  +    ++DI + F  GDHGDG PFDG  G LAHAF P  
Sbjct: 189 KSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPS 248

Query: 247 GRIHFDADEPWERGPILEQF-------DVESVALHELGHALGLGHSNIESAVMWPDMDSG 296
           G+ H DADE W     L+ F       D+ESVA+HE+GH LGLGHS++E ++M+P + +G
Sbjct: 249 GKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTG 308

[Close]

BLAST of Lsi02G015340 vs. TAIR10
Match: AT1G24140.1 (AT1G24140.1 Matrixin family protein)

HSP 1 Score: 201.8 bits (512), Expect = 5.8e-52
Identity = 111/279 (39.78%), Postives = 160/279 (57.35%), Query Frame = 1


Query: 42  GSRKDDNIEGIHKVKAYLQRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVL 101
           G       +G++ +K Y Q +GY++  + +   TD FD+ L++A++ YQ  F+LNV+GVL
Sbjct: 51  GCHAGKKYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVL 110

Query: 102 DKETLQQMSKPRCGVTD-----NFIVSQGKNVEMNGS----------HYSFFPKKRTWPV 161
           D+ TL+ +  PRCG  D     + + S  K  E++ +          HYSFFP +  WP 
Sbjct: 111 DELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPR 170

Query: 162 EKKHLTYGFI-HNYPPEHVAAVM-RALETWADNTKFTFAQAARVQSADILLSFERGDHGD 221
            ++ LTY F   N   E V +V  RA   W + T  TF +  R  ++DI + F  G+HGD
Sbjct: 171 NRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGD 230

Query: 222 GRPFDGEGGVLAHAFGPIDGRIHFDADEPW----ERGP----ILEQFDVESVALHELGHA 281
           G PFDG    LAHAF P  G  H D +E W    E G     + E  D+ESVA+HE+GH 
Sbjct: 231 GEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHL 290

Query: 282 LGLGHSNIESAVMWPDMDSGVTKTRLTIDDIEGIRALYG 296
           LGLGHS++E ++M+P + +G  K  LT DD+EG++ LYG
Sbjct: 291 LGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYG 329

[Close]

BLAST of Lsi02G015340 vs. TAIR10
Match: AT4G16640.1 (AT4G16640.1 Matrixin family protein)

HSP 1 Score: 184.9 bits (468), Expect = 7.3e-47
Identity = 102/259 (39.38%), Postives = 145/259 (55.98%), Query Frame = 1


Query: 48  NIEGIHKVKAYLQRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQ 107
           ++ G+ ++K YL R+GY+++ SE  + +D FD  LESAI  YQ    L ++G LD  T+ 
Sbjct: 66  HVSGVSELKRYLHRFGYVNDGSE--IFSDVFDGPLESAISLYQENLGLPITGRLDTSTVT 125

Query: 108 QMSKPRCGVTDNFIVSQGKNVEMNGSHYSFFPKKRTWPVEKKHLTYGFIHNYPPEHVAA- 167
            MS PRCGV+D  +      +    +HY++F  K  W   +  LTY     +  +++ + 
Sbjct: 126 LMSLPRCGVSDTHMTINNDFLHTT-AHYTYFNGKPKW--NRDTLTYAISKTHKLDYLTSE 185

Query: 168 -----VMRALETWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGP 227
                  RA   W+     +F +     +AD+ + F  GDHGDG PFDG  G LAHAF P
Sbjct: 186 DVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAP 245

Query: 228 IDGRIHFDADEPW-----ERGPILEQFDVESVALHELGHALGLGHSNIESAVMWPDMDSG 287
            +GR+H DA E W      +G      D+ESVA HE+GH LGLGHS+ ESAVM+P +   
Sbjct: 246 ENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPR 305

Query: 288 VTKTRLTIDDIEGIRALYG 296
             K  LT+DD+ G+  LYG
Sbjct: 306 TKKVDLTVDDVAGVLKLYG 319

[Close]

BLAST of Lsi02G015340 vs. TAIR10
Match: AT2G45040.1 (AT2G45040.1 Matrixin family protein)

HSP 1 Score: 163.3 bits (412), Expect = 2.3e-40
Identity = 101/284 (35.56%), Postives = 141/284 (49.65%), Query Frame = 1


Query: 26  LHNHKSSRLLFPKHLQGSRKDDNIEGIHKVKAYLQRYGYLSNKSENKLNTDSFDEDLESA 85
           LHN +      P     +   D    I ++K +LQ+YGYL        N +S D   E A
Sbjct: 26  LHNQQIIEARNPSQFTTNPSPD--VSIPEIKRHLQQYGYLPQ------NKESDDVSFEQA 85

Query: 86  IKSYQTFFKLNVSGVLDKETLQQMSKPRCGVTDNFIVSQGKNVEMN-GSHYSFFPKKRTW 145
           +  YQ    L ++G  D +TL Q+  PRCG  D+    + K    + G  Y +FP +  W
Sbjct: 86  LVRYQKNLGLPITGKPDSDTLSQILLPRCGFPDDV---EPKTAPFHTGKKYVYFPGRPRW 145

Query: 146 PVEKK-HLTYGFIHNYPPEHVAAV------MRALETWADNTKFTFAQAARVQSADILLSF 205
             +    LTY F       ++A         RA   WA     +F +      ADI + F
Sbjct: 146 TRDVPLKLTYAFSQENLTPYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGF 205

Query: 206 ERGDHGDGRPFDGEGGVLAHAFGPIDGRIHFDADEPW------ERGPILEQFDVESVALH 265
             GDHGDG PFDG  GVLAH F P +GR+H D  E W      E+  +    D+ESVA+H
Sbjct: 206 FNGDHGDGEPFDGVLGVLAHTFSPENGRLHLDKAETWAVDFDEEKSSV--AVDLESVAVH 265

Query: 266 ELGHALGLGHSNIESAVMWPDMDSGVTKTRLTIDDIEGIRALYG 296
           E+GH LGLGHS+++ A M+P +     K  L +DD+ G+++LYG
Sbjct: 266 EIGHVLGLGHSSVKDAAMYPTLKPRSKKVNLNMDDVVGVQSLYG 296

[Close]

BLAST of Lsi02G015340 vs. NCBI nr
Match: gi|659118025|ref|XP_008458911.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 468.0 bits (1203), Expect = 1.2e-128
Identity = 226/303 (74.59%), Postives = 252/303 (83.17%), Query Frame = 1


Query: 1   MASKLCLQLFLLLAFITPHLTSTRPLHNHKSSRLLFPKHLQGSRKDDNIEGIHKVKAYLQ 60
           M SKLCLQLFLL AFITPHLTS R L NHK SRLLFP+HL GSRK+DN EGIHKVKAYLQ
Sbjct: 1   MGSKLCLQLFLLFAFITPHLTSARHLRNHKPSRLLFPEHLIGSRKNDNFEGIHKVKAYLQ 60

Query: 61  RYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQQMSKPRCGVTDNF 120
           RYGYLS++ EN L+ D+FD+DLESAIKSYQ  FKLNVSGVLD+ETLQQM++PRCGVTD+F
Sbjct: 61  RYGYLSDEKENNLSIDAFDDDLESAIKSYQKVFKLNVSGVLDRETLQQMARPRCGVTDDF 120

Query: 121 IV-------SQGKNVEMNGSHYSFFPKKRTWPVEKKHLTYGFIHNYPPEHVAAVMRALET 180
           I+       +   N+++ GSHY F+P K  WPVEK+HLTYGFIHNYPPEH AAV+RA +T
Sbjct: 121 ILVAQQDHENNNTNIQIGGSHYMFYPNKLKWPVEKRHLTYGFIHNYPPEHAAAVIRAFDT 180

Query: 181 WADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPIDGRIHFDADEP 240
           W  NTKFTF+ AARVQ+ADILLSFERGDHGDG+PFDG GG L HAFG IDGR+HFD DE 
Sbjct: 181 WEANTKFTFSHAARVQTADILLSFERGDHGDGKPFDGPGGALGHAFGAIDGRVHFDGDEQ 240

Query: 241 WERGPI-LEQFDVESVALHELGHALGLGHSNIESAVMWPDMDSGVTKTRLTIDDIEGIRA 296
           WE G    E FDVESVALHE+GHALGLGHS   SAVMW +MDSGVTKT LTIDDIEGI A
Sbjct: 241 WETGSFWQEYFDVESVALHEIGHALGLGHSREPSAVMWANMDSGVTKTHLTIDDIEGIHA 300

[Close]

BLAST of Lsi02G015340 vs. NCBI nr
Match: gi|778708735|ref|XP_004142466.2| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 439.9 bits (1130), Expect = 3.6e-120
Identity = 219/303 (72.28%), Postives = 253/303 (83.50%), Query Frame = 1


Query: 1   MASKLCLQLFLLLAFITPHLTSTRPLHNHKSSRLLFPKHLQGSRKDDNI-EGIHKVKAYL 60
           MA+KLCLQLFLLLAF+TPHLTS RPLHNHKSSRLLFP+HL GS K+D+I EGIHKVKAYL
Sbjct: 1   MAAKLCLQLFLLLAFVTPHLTSARPLHNHKSSRLLFPEHLIGSSKNDSIVEGIHKVKAYL 60

Query: 61  QRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQQMSKPRCGVTDN 120
           QRYGY SN++EN L+TD+FD+DLESAIKSYQ F  L VSGVLD+ETLQQMS+PRCGV DN
Sbjct: 61  QRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVADN 120

Query: 121 F-IVSQ--GKN----VEMNGSHYSFFPKKRTWPVEKKHLTYGFIHNYPPEHVAAVMRALE 180
           F  V+Q  G+N    V++ GSH+ FFP K  WP  K HLTYGF+HNYP +H AAV+RA +
Sbjct: 121 FQSVAQQDGENNNTTVQIGGSHFMFFPGKGKWPYRKWHLTYGFVHNYPMKHAAAVVRAFD 180

Query: 181 TWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPIDGRIHFDADE 240
            WA N+KFTF+ A R+Q+ADILLSFERGDHGDG+PFDGEGG+L HAFGPIDGR+HFDADE
Sbjct: 181 KWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAFGPIDGRVHFDADE 240

Query: 241 PW-ERGPIL-EQFDVESVALHELGHALGLGHSNIESAVMWPDMDSGVTKTRLTIDDIEGI 294
            W E G +  E FD ESVALHE+GHALGLGHS   SAVMW +M++GV KT LTIDDIEG+
Sbjct: 241 QWAEIGSLTNENFDFESVALHEIGHALGLGHSIFPSAVMWANMETGVNKTELTIDDIEGV 300

[Close]

BLAST of Lsi02G015340 vs. NCBI nr
Match: gi|659118029|ref|XP_008458913.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 439.5 bits (1129), Expect = 4.6e-120
Identity = 216/303 (71.29%), Postives = 246/303 (81.19%), Query Frame = 1


Query: 1   MASKLCLQLFLLLAFITPHLTSTRPLHNHKSSRLLFPKHLQGSRKDDNI-EGIHKVKAYL 60
           MASKLCLQLFLLLAF+TPHLTS R LHNHK SRLLFP+HL GS K+DNI EGIHKVKAYL
Sbjct: 1   MASKLCLQLFLLLAFVTPHLTSARHLHNHKPSRLLFPEHLIGSSKNDNIVEGIHKVKAYL 60

Query: 61  QRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQQMSKPRCGVTDN 120
           QRYGY SN++ N L+TD FD DLESAIKSYQ F KL VSGVLD+ETLQQMSKPRCGV D 
Sbjct: 61  QRYGYFSNENANTLSTDVFDNDLESAIKSYQKFSKLKVSGVLDRETLQQMSKPRCGVRDT 120

Query: 121 FIV-------SQGKNVEMNGSHYSFFPKKRTWPVEKKHLTYGFIHNYPPEHVAAVMRALE 180
           F+        ++  N+E+ GSHY+F+    TWP EKKHL+YGFIHNYPP+ V  ++RA E
Sbjct: 121 FVSVAQQEHENKNTNIEIGGSHYTFYFNNVTWPAEKKHLSYGFIHNYPPQFVDPLVRAFE 180

Query: 181 TWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPIDGRIHFDADE 240
           TW DNTKF+F  A RVQ+ADIL+SFERGDHGD + FDGEGG+LAH+ G +DGR+HFDADE
Sbjct: 181 TWEDNTKFSFFLAPRVQTADILVSFERGDHGDYQAFDGEGGILAHSLGAVDGRVHFDADE 240

Query: 241 PWERGPILEQFDVESVALHELGHALGLGHSNIESAVMWPDMDSGVTKTRLTIDDIEGIRA 296
           PWE GPI ++ D+ESVALHELGH LGLGHS   SAVM+  MD GV KT LTIDDIEGI A
Sbjct: 241 PWESGPIEDRVDLESVALHELGHTLGLGHSREPSAVMYAFMDPGVYKTHLTIDDIEGICA 300

[Close]

BLAST of Lsi02G015340 vs. NCBI nr
Match: gi|659118027|ref|XP_008458912.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 438.7 bits (1127), Expect = 7.9e-120
Identity = 210/303 (69.31%), Postives = 248/303 (81.85%), Query Frame = 1


Query: 1   MASKLCLQLFLLLAFITPHLTSTRPLHNHKSSRLLFPKHLQGSRKDDNI-EGIHKVKAYL 60
           MASKLCLQLFLLLAFITPHLTS R LHNHK S  LFPKHL GS K+D+I EGIHKVKAYL
Sbjct: 1   MASKLCLQLFLLLAFITPHLTSARHLHNHKPSPFLFPKHLIGSSKNDSIVEGIHKVKAYL 60

Query: 61  QRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQQMSKPRCGVTDN 120
           QRYGYLSN++EN L+TD+FD+DLESAIKSYQ F K+ VSGVLD+ETLQQMS PRCGV D 
Sbjct: 61  QRYGYLSNENENNLSTDAFDDDLESAIKSYQKFSKIKVSGVLDRETLQQMSTPRCGVRDT 120

Query: 121 FIV-------SQGKNVEMNGSHYSFFPKKRTWPVEKKHLTYGFIHNYPPEHVAAVMRALE 180
           F+        ++  N+E+ GSHY+F+     WP EKKHL+YGFIHN+PP+HV  V+RA +
Sbjct: 121 FVSVAQQEHENKSTNIEIGGSHYTFYYNNVKWPAEKKHLSYGFIHNFPPQHVPTVLRAFQ 180

Query: 181 TWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPIDGRIHFDADE 240
           TW DNTKF+F  A RVQ+ADIL+SFERG+HGD  PFDG GG+LAH+ G +DGR+HFDADE
Sbjct: 181 TWEDNTKFSFFLAPRVQTADILVSFERGEHGDDEPFDGPGGILAHSLGAVDGRVHFDADE 240

Query: 241 PWERGPILEQFDVESVALHELGHALGLGHSNIESAVMWPDMDSGVTKTRLTIDDIEGIRA 296
            WE GP+ +++D+E++ALHELGHALGLGHS+   AVMW  M +G  KTRLTIDDIEGIRA
Sbjct: 241 QWEGGPVEKRYDLETLALHELGHALGLGHSSSSLAVMWAYMYAGFPKTRLTIDDIEGIRA 300

[Close]

BLAST of Lsi02G015340 vs. NCBI nr
Match: gi|449449429|ref|XP_004142467.1| (PREDICTED: matrix metalloproteinase-9-like [Cucumis sativus])

HSP 1 Score: 419.5 bits (1077), Expect = 5.0e-114
Identity = 202/303 (66.67%), Postives = 245/303 (80.86%), Query Frame = 1


Query: 1   MASKLCLQLFLLLAFITPHLTSTRPLHNHKSSRLLFPKHLQGSRKDDNI-EGIHKVKAYL 60
           MASKLCLQL LLLAFITPHLTS R LHN K S  LFPKHL GS K+D+I EGIHKVKAYL
Sbjct: 1   MASKLCLQLCLLLAFITPHLTSARHLHNRKPSPFLFPKHLIGSSKNDSIVEGIHKVKAYL 60

Query: 61  QRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQQMSKPRCGVTDN 120
           QRYGYLSN++E KL+TD+FD+DLESAIKSYQ F  L VSGVLD+ETLQQMS+PRCGV D 
Sbjct: 61  QRYGYLSNENEKKLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVRDT 120

Query: 121 FIV-------SQGKNVEMNGSHYSFFPKKRTWPVEKKHLTYGFIHNYPPEHVAAVMRALE 180
           ++        ++  N+E+ GSHY+F+    TWP E++HL+YGFIH++PP+HV  V RA +
Sbjct: 121 YVSVGQQEHENKNTNIEIGGSHYTFYYNHVTWPAERRHLSYGFIHDFPPQHVDTVRRAFQ 180

Query: 181 TWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPIDGRIHFDADE 240
           TW DNTKF+F  + RVQ+ADIL+SFERG+HGD  PFDGEGG+LAH+ G +DGR+HFDAD+
Sbjct: 181 TWEDNTKFSFFLSPRVQTADILVSFERGEHGDHEPFDGEGGILAHSLGAVDGRVHFDADD 240

Query: 241 PWERGPILEQFDVESVALHELGHALGLGHSNIESAVMWPDMDSGVTKTRLTIDDIEGIRA 296
            WE  P +E++D+E++ALHE+GHALGLGHS+   A+MW  M  G  KTRLTIDDIEGIRA
Sbjct: 241 RWEGDP-MERYDLETLALHEIGHALGLGHSSSSLAIMWAYMYKGFPKTRLTIDDIEGIRA 300

The following BLAST results are available for this feature:

BLAST of Lsi02G015340 vs. Swiss-Prot


Match Name	E-value	Identity	Description
5MMP_ARATH	5.2e-55	42.96	Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1	[more]
2MMP_ARATH	3.7e-53	39.50	Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1	[more]
3MMP_ARATH	1.0e-50	39.78	Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1	[more]
MEP1_SOYBN	1.2e-48	41.25	Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2	[more]
1MMP_ARATH	1.3e-45	39.38	Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1	[more]

BLAST of Lsi02G015340 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KS35_CUCSA	2.5e-120	72.28	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632070 PE=3 SV=1	[more]
A0A0A0KVC8_CUCSA	3.5e-114	66.67	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632060 PE=3 SV=1	[more]
A0A0A0KU02_CUCSA	1.1e-78	53.49	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632080 PE=3 SV=1	[more]
A0A0A0KNY8_CUCSA	8.4e-76	50.33	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G522910 PE=3 SV=1	[more]
B9RUG6_RICCO	2.7e-74	49.03	Metalloendoproteinase 1, putative OS=Ricinus communis GN=RCOM_0852690 PE=3 SV=1	[more]

BLAST of Lsi02G015340 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G59970.1	2.9e-56	42.96	Matrixin family protein	[more]
AT1G70170.1	2.1e-54	39.50	matrix metalloproteinase	[more]
AT1G24140.1	5.8e-52	39.78	Matrixin family protein	[more]
AT4G16640.1	7.3e-47	39.38	Matrixin family protein	[more]
AT2G45040.1	2.3e-40	35.56	Matrixin family protein	[more]

BLAST of Lsi02G015340 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659118025\|ref\|XP_008458911.1\|	1.2e-128	74.59	PREDICTED: metalloendoproteinase 1-like [Cucumis melo]	[more]
gi\|778708735\|ref\|XP_004142466.2\|	3.6e-120	72.28	PREDICTED: metalloendoproteinase 1-like [Cucumis sativus]	[more]
gi\|659118029\|ref\|XP_008458913.1\|	4.6e-120	71.29	PREDICTED: metalloendoproteinase 1-like [Cucumis melo]	[more]
gi\|659118027\|ref\|XP_008458912.1\|	7.9e-120	69.31	PREDICTED: metalloendoproteinase 1-like [Cucumis melo]	[more]
gi\|449449429\|ref\|XP_004142467.1\|	5.0e-114	66.67	PREDICTED: matrix metalloproteinase-9-like [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0008237	metallopeptidase activity
GO:0008270	zinc ion binding
GO:0004222	metalloendopeptidase activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0031012	extracellular matrix

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

Vocabulary: **INTERPRO**
Term	Definition
IPR024079	MetalloPept_cat_dom_sf
IPR021190	Pept_M10A
IPR006026	Peptidase_Metallo
IPR002477	Peptidoglycan-bd-like
IPR001818	Pept_M10_metallopeptidase

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0031012	extracellular matrix
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0008237	metallopeptidase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G015340.1	Lsi02G015340.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 147..295 score: 2.1
IPR002477	Peptidoglycan binding-like	GENE3D	G3DSA:1.10.101.10		coord: 51..110 score: 1.7
IPR002477	Peptidoglycan binding-like	PFAM	PF01471	PG_binding_1	coord: 50..109 score: 8.1
IPR002477	Peptidoglycan binding-like	unknown	SSF47090	PGBD-like	coord: 46..118 score: 1.44
IPR006026	Peptidase, metallopeptidase	SMART	SM00235	col_5	coord: 140..296 score: 6.6
IPR021190	Peptidase M10A	PRINTS	PR00138	MATRIXIN	coord: 109..122 score: 1.6E-36coord: 191..219 score: 1.6E-36coord: 167..182 score: 1.6E-36coord: 282..295 score: 1.6E-36coord: 248..273 score: 1.6
IPR024079	Metallopeptidase, catalytic domain	GENE3D	G3DSA:3.40.390.10		coord: 111..295 score: 1.7
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 37..296 score: 2.8E-113coord: 1..21 score: 2.8E
None	No IPR available	PANTHER	PTHR10201:SF152	SUBFAMILY NOT NAMED	coord: 37..296 score: 2.8E-113coord: 1..21 score: 2.8E
None	No IPR available	unknown	SSF55486	Metalloproteases ("zincins"), catalytic domain	coord: 137..295 score: 2.69

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi02G015340	CmaCh15G003110	Cucurbita maxima (Rimu)	cmalsiB272
Lsi02G015340	CmoCh15G003230	Cucurbita moschata (Rifu)	cmolsiB261
Lsi02G015340	Cla010373	Watermelon (97103) v1	lsiwmB170
Lsi02G015340	MELO3C021504.2	Melon (DHL92) v3.6.1	lsimedB171
Lsi02G015340	CsGy5G027760	Cucumber (Gy14) v2	cgyblsiB319
Lsi02G015340	CsaV3_5G037510	Cucumber (Chinese Long) v3	cuclsiB371

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi02G015340	Bottle gourd (USVL1VR-Ls)	lsilsiB089
Lsi02G015340	Cucumber (Gy14) v1	cgylsiB118
Lsi02G015340	Cucumber (Gy14) v1	cgylsiB251
Lsi02G015340	Cucurbita maxima (Rimu)	cmalsiB372
Lsi02G015340	Cucurbita maxima (Rimu)	cmalsiB651
Lsi02G015340	Cucurbita moschata (Rifu)	cmolsiB640
Lsi02G015340	Cucurbita pepo (Zucchini)	cpelsiB141
Lsi02G015340	Cucurbita pepo (Zucchini)	cpelsiB336
Lsi02G015340	Wild cucumber (PI 183967)	cpilsiB028
Lsi02G015340	Wild cucumber (PI 183967)	cpilsiB352
Lsi02G015340	Cucumber (Chinese Long) v2	culsiB028
Lsi02G015340	Cucumber (Chinese Long) v2	culsiB348
Lsi02G015340	Melon (DHL92) v3.5.1	lsimeB162
Lsi02G015340	Melon (DHL92) v3.5.1	lsimeB182
Lsi02G015340	Watermelon (Charleston Gray)	lsiwcgB155
Lsi02G015340	Watermelon (Charleston Gray)	lsiwcgB176
Lsi02G015340	Cucumber (Gy14) v2	cgyblsiB024
Lsi02G015340	Melon (DHL92) v3.6.1	lsimedB191
Lsi02G015340	Silver-seed gourd	carlsiB232
Lsi02G015340	Silver-seed gourd	carlsiB623
Lsi02G015340	Cucumber (Chinese Long) v3	cuclsiB028
Lsi02G015340	Watermelon (97103) v2	lsiwmbB161
Lsi02G015340	Watermelon (97103) v2	lsiwmbB208
Lsi02G015340	Wax gourd	lsiwgoB207
Lsi02G015340	Wax gourd	lsiwgoB253