Lsi02G015330 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi02G015330
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionMatrix metalloproteinase
Locationchr02 : 21199942 .. 21200826 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCTTAAGTTCTTTCTTCAACTCTTCCTCCTCTTTGCCTCCGTCGCACCTCACATGATCACGGCGCGTGTTCTTCACACCAACATATCCTCCCATCTTCAAGGAAGTCGTATAGGTAACAATACCGAAGGAATCCACAACGTCAAGCTTTACCTCCAACGTTATGGTTACTTAACCAACGTAAAGAGTACCAATCCTAATGTATTCGACGATCTCCTAGAATCAGCCATTAAAACATTCCAAAAATACCATAGCCTCAACGTGAGTGGCATTTTGGATAAGGAGACACTAGCTCTAATGTCTCAGCCTCGATGTGGAATTCCAGATATCTTGCACAATAATAATATCACTCATAGTGGCATTCAAATAAATAGTACCACCTTTCATTCTCATTTCTCATTCTTTCCAGGAAGTCCAAAATGGCCGATATCGAAATACCATTTGACGTACAGGTTTCTCGATAGTTTTCCAAATAACTTCGTAGCACCGGTGACGAACGCAATGGAGCAATGGGGGAAGTTCAGCAAGTTCACATTCTCAGTAGCCGCAAGGTCTCAAAAGGCAGACATCACATTTAACTTTGTGAGAGGAAACCATGGGGATGGCTATCCATTTGAGGGAAAAGGAGGGACTTTGGCGCATGCTTTTGGGCCACCAGATGGGAGGGTACACTTTGATGCGGACGAAAGTTGGGTGGATGGGTCTGTTAGTGGTTTCTTCAATGTGGGGATGGTGGCGTTGCATGAGCTTGGGCATGTGCTTGGGCTTGGCCACAGCACCATTCGAGACGCCATTATGTGGCCCTACATGAACCCCGGTGATCAGTTCAGGGGCTTACAGTTTGATGATATTCAAGGCATCCAAACTTTGTATAGTTCTTGA

mRNA sequence

ATGGCTCTTAAGTTCTTTCTTCAACTCTTCCTCCTCTTTGCCTCCGTCGCACCTCACATGATCACGGCGCGTGTTCTTCACACCAACATATCCTCCCATCTTCAAGGAAGTCGTATAGGTAACAATACCGAAGGAATCCACAACGTCAAGCTTTACCTCCAACGTTATGGTTACTTAACCAACGTAAAGAGTACCAATCCTAATGTATTCGACGATCTCCTAGAATCAGCCATTAAAACATTCCAAAAATACCATAGCCTCAACGTGAGTGGCATTTTGGATAAGGAGACACTAGCTCTAATGTCTCAGCCTCGATGTGGAATTCCAGATATCTTGCACAATAATAATATCACTCATAGTGGCATTCAAATAAATAGTACCACCTTTCATTCTCATTTCTCATTCTTTCCAGGAAGTCCAAAATGGCCGATATCGAAATACCATTTGACGTACAGGTTTCTCGATAGTTTTCCAAATAACTTCGTAGCACCGGTGACGAACGCAATGGAGCAATGGGGGAAGTTCAGCAAGTTCACATTCTCAGTAGCCGCAAGGTCTCAAAAGGCAGACATCACATTTAACTTTGTGAGAGGAAACCATGGGGATGGCTATCCATTTGAGGGAAAAGGAGGGACTTTGGCGCATGCTTTTGGGCCACCAGATGGGAGGGTACACTTTGATGCGGACGAAAGTTGGGTGGATGGGTCTGTTAGTGGTTTCTTCAATGTGGGGATGGTGGCGTTGCATGAGCTTGGGCATGTGCTTGGGCTTGGCCACAGCACCATTCGAGACGCCATTATGTGGCCCTACATGAACCCCGGTGATCAGTTCAGGGGCTTACAGTTTGATGATATTCAAGGCATCCAAACTTTGTATAGTTCTTGA

Coding sequence (CDS)

ATGGCTCTTAAGTTCTTTCTTCAACTCTTCCTCCTCTTTGCCTCCGTCGCACCTCACATGATCACGGCGCGTGTTCTTCACACCAACATATCCTCCCATCTTCAAGGAAGTCGTATAGGTAACAATACCGAAGGAATCCACAACGTCAAGCTTTACCTCCAACGTTATGGTTACTTAACCAACGTAAAGAGTACCAATCCTAATGTATTCGACGATCTCCTAGAATCAGCCATTAAAACATTCCAAAAATACCATAGCCTCAACGTGAGTGGCATTTTGGATAAGGAGACACTAGCTCTAATGTCTCAGCCTCGATGTGGAATTCCAGATATCTTGCACAATAATAATATCACTCATAGTGGCATTCAAATAAATAGTACCACCTTTCATTCTCATTTCTCATTCTTTCCAGGAAGTCCAAAATGGCCGATATCGAAATACCATTTGACGTACAGGTTTCTCGATAGTTTTCCAAATAACTTCGTAGCACCGGTGACGAACGCAATGGAGCAATGGGGGAAGTTCAGCAAGTTCACATTCTCAGTAGCCGCAAGGTCTCAAAAGGCAGACATCACATTTAACTTTGTGAGAGGAAACCATGGGGATGGCTATCCATTTGAGGGAAAAGGAGGGACTTTGGCGCATGCTTTTGGGCCACCAGATGGGAGGGTACACTTTGATGCGGACGAAAGTTGGGTGGATGGGTCTGTTAGTGGTTTCTTCAATGTGGGGATGGTGGCGTTGCATGAGCTTGGGCATGTGCTTGGGCTTGGCCACAGCACCATTCGAGACGCCATTATGTGGCCCTACATGAACCCCGGTGATCAGTTCAGGGGCTTACAGTTTGATGATATTCAAGGCATCCAAACTTTGTATAGTTCTTGA

Protein sequence

MALKFFLQLFLLFASVAPHMITARVLHTNISSHLQGSRIGNNTEGIHNVKLYLQRYGYLTNVKSTNPNVFDDLLESAIKTFQKYHSLNVSGILDKETLALMSQPRCGIPDILHNNNITHSGIQINSTTFHSHFSFFPGSPKWPISKYHLTYRFLDSFPNNFVAPVTNAMEQWGKFSKFTFSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHAFGPPDGRVHFDADESWVDGSVSGFFNVGMVALHELGHVLGLGHSTIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLYSS
Homology
[Close]
BLAST of Lsi02G015330 vs. Swiss-Prot
Match: 2MMP_ARATH (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.1e-52
Identity = 114/318 (35.85%), Postives = 178/318 (55.97%), Query Frame = 1 

Query: 6   FLQLFLLFA-----------SVAPHMITARVLHTNISSHLQGSRIGNNTEGIHNVKLYLQ 65
           FL LFL+ +           +V P +     +  +  S+  G   G N +G++ +K Y Q
Sbjct: 8   FLSLFLIVSPASAWFFPNSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKKYFQ 67

Query: 66  RYGYLTNVKSTN-PNVFDDLLESAIKTFQKYHSLNVSGILDKETLALMSQPRCGIPDILH 125
           R+GY+    S N  + FDD+L++A++ +Q   +LNV+G LD  T+  +  PRCG PD+++
Sbjct: 68  RFGYIPETFSGNFTDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNPDVVN 127

Query: 126 NNNITHSG----IQINSTTFHSH----FSFFPGSPKWPISKYHLTYRFLDSFP--NNFVA 185
             ++ H G     ++N +  H H    ++ FPG P+WP ++  LTY F    P      +
Sbjct: 128 GTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEVKS 187

Query: 186 PVTNAMEQWGKFSKFTFSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHAFGPPDGR 245
             + A  +W   +   F+++     +DIT  F  G+HGDG PF+G  GTLAHAF PP G+
Sbjct: 188 VFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGK 247

Query: 246 VHFDADESW-VDGSVSGFFNV------GMVALHELGHVLGLGHSTIRDAIMWPYMNPGDQ 295
            H DADE+W V G +  F +V        VA+HE+GH+LGLGHS++ ++IM+P +  G +
Sbjct: 248 FHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKR 307

[Close]
BLAST of Lsi02G015330 vs. Swiss-Prot
Match: 5MMP_ARATH (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 1.8e-52
Identity = 112/274 (40.88%), Postives = 158/274 (57.66%), Query Frame = 1 

Query: 32  SHLQGSRIGNNTEGIHNVKLYLQRYGYLTNVKSTNPNVFDDLLESAIKTFQKYHSLNVSG 91
           S L G  IG N  G+  +K Y +R+GY+T   +   + FDD+L+SAI T+QK  +L V+G
Sbjct: 46  SKLAGCHIGENINGLSKLKQYFRRFGYITTTGNCTDD-FDDVLQSAINTYQKNFNLKVTG 105

Query: 92  ILDKETLALMSQPRCGIPDILHNNNITHSGIQINSTTFHSHFSFFPGSPKWPISKYHLTY 151
            LD  TL  + +PRCG PD++   +  + G  + +T     +SFFPG P+WP  K  LTY
Sbjct: 106 KLDSSTLRQIVKPRCGNPDLIDGVSEMNGGKILRTT---EKYSFFPGKPRWPKRKRDLTY 165

Query: 152 RFLDSFPNNFVAPV----TNAMEQWGKFSKFTFSVAARSQKADITFNFVRGNHGDGYPFE 211
            F     NN    V    + A  +W + +   F+ +    +ADI   F  G HGDG PF+
Sbjct: 166 AFAPQ--NNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFD 225

Query: 212 GKGGTLAHAFGPPDGRVHFDADESWVDGS---------VSGFFNVGMVALHELGHVLGLG 271
           G  GTLAHA  PP G +H D DE W+  +         V+   ++  VA+HE+GH+LGLG
Sbjct: 226 GAMGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLG 285

Query: 272 HSTIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLY 293
           HS++ DAIM+P ++ GD+   L  DDI+GIQ LY
Sbjct: 286 HSSVEDAIMFPAISGGDRKVELAKDDIEGIQHLY 313

[Close]
BLAST of Lsi02G015330 vs. Swiss-Prot
Match: 3MMP_ARATH (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 3.5e-51
Identity = 104/280 (37.14%), Postives = 162/280 (57.86%), Query Frame = 1 

Query: 36  GSRIGNNTEGIHNVKLYLQRYGYLT--NVKSTNPNVFDDLLESAIKTFQKYHSLNVSGIL 95
           G   G   +G++ +K Y Q +GY+T  N+     + FDD+L++A++ +Q+   LNV+G+L
Sbjct: 51  GCHAGKKYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVL 110

Query: 96  DKETLALMSQPRCGIPDILHNNNITHSGIQINSTTFHS---------HFSFFPGSPKWPI 155
           D+ TL  +  PRCG PD+++  +  HSG +    +F           H+SFFPG P+WP 
Sbjct: 111 DELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPR 170

Query: 156 SKYHLTYRF--LDSFPNNFVAPVTNAMEQWGKFSKFTFSVAARSQKADITFNFVRGNHGD 215
           ++  LTY F   ++      +  + A  +W + +  TF+   R   +DI+  F  G HGD
Sbjct: 171 NRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGD 230

Query: 216 GYPFEGKGGTLAHAFGPPDGRVHFDADESWV------DG--SVSGFFNVGMVALHELGHV 275
           G PF+G   TLAHAF PP G  H D +E+W+      DG  SVS   ++  VA+HE+GH+
Sbjct: 231 GEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHL 290

Query: 276 LGLGHSTIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLYSS 295
           LGLGHS++  +IM+P +  G +   L  DD++G+Q LY +
Sbjct: 291 LGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYGA 330

[Close]
BLAST of Lsi02G015330 vs. Swiss-Prot
Match: MEP1_SOYBN (Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2)

HSP 1 Score: 197.6 bits (501), Expect = 1.9e-49
Identity = 110/261 (42.15%), Postives = 142/261 (54.41%), Query Frame = 1 

Query: 40  GNNTEGIHNVKLYLQRYGYLTNVKSTNPNVFDDLLESAIKTFQKYHSLNVSGILDKETLA 99
           G N +G+ NVK Y    GY+ N    + N FDD L SAIKT+QK ++LNV+G  D  TL 
Sbjct: 48  GQNYKGLSNVKNYFHHLGYIPNAPHFDDN-FDDTLVSAIKTYQKNYNLNVTGKFDINTLK 107

Query: 100 LMSQPRCGIPDILHNNNITHSGIQINSTTFHSHFSFFPGSPKWPISKYHLTYRFLDS--F 159
            +  PRCG+PDI+ N N T S   I      S ++FF   P+W      LTY F      
Sbjct: 108 QIMTPRCGVPDIIINTNKTTSFGMI------SDYTFFKDMPRWQAGTTQLTYAFSPEPRL 167

Query: 160 PNNFVAPVTNAMEQWGKFSKFTFSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHAF 219
            + F + +  A  +W       F      + A+I   F   NHGD YPF+G GG L HAF
Sbjct: 168 DDTFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGHAF 227

Query: 220 GPPDGRVHFDADESWV------DGSVSGFFNVGMVALHELGHVLGLGHSTIRDAIMWPYM 279
            P DGR HFDADE WV         V+  F++  VA+HE+GH+LGLGHS+   AIM+P +
Sbjct: 228 APTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYPSI 287

Query: 280 NPGDQFRGLQFDDIQGIQTLY 293
            P  +   L  DDI GI+ LY
Sbjct: 288 PPRTRKVNLAQDDIDGIRKLY 301

[Close]
BLAST of Lsi02G015330 vs. Swiss-Prot
Match: 1MMP_ARATH (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 4.1e-44
Identity = 105/272 (38.60%), Postives = 146/272 (53.68%), Query Frame = 1 

Query: 32  SHLQGSRIGNNTEGIHNVKLYLQRYGYLTNVKSTNPNVFDDLLESAIKTFQKYHSLNVSG 91
           S L   +IG++  G+  +K YL R+GY+ +      +VFD  LESAI  +Q+   L ++G
Sbjct: 56  SRLVDVQIGSHVSGVSELKRYLHRFGYVNDGSEIFSDVFDGPLESAISLYQENLGLPITG 115

Query: 92  ILDKETLALMSQPRCGIPDILHNNNITHSGIQINSTTFHSHFSFFPGSPKWPISKYHLTY 151
            LD  T+ LMS PRCG+ D       TH  I  +     +H+++F G PKW  ++  LTY
Sbjct: 116 RLDTSTVTLMSLPRCGVSD-------THMTINNDFLHTTAHYTYFNGKPKW--NRDTLTY 175

Query: 152 RF-----LDSFPNNFVAPV-TNAMEQWGKFSKFTFSVAARSQKADITFNFVRGNHGDGYP 211
                  LD   +  V  V   A  QW      +F        AD+   F  G+HGDG P
Sbjct: 176 AISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLP 235

Query: 212 FEGKGGTLAHAFGPPDGRVHFDADESW-VDGSVSGFFNVGM----VALHELGHVLGLGHS 271
           F+G  GTLAHAF P +GR+H DA E+W VD  + G   V +    VA HE+GH+LGLGHS
Sbjct: 236 FDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHS 295

Query: 272 TIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLY 293
           +   A+M+P + P  +   L  DD+ G+  LY
Sbjct: 296 SQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]
BLAST of Lsi02G015330 vs. TrEMBL
Match: A0A0A0KU02_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632080 PE=3 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 4.1e-131
Identity = 227/294 (77.21%), Postives = 254/294 (86.39%), Query Frame = 1 

Query: 1   MALKFFLQLFLLFASVAPHMITARVLHTNISSHLQGSRIGNNTEGIHNVKLYLQRYGYLT 60
           MAL+F LQLFLL AS+APH+ITARV HT+ISSHLQGSRIGNN EGIHNVKLYL+RYGYLT
Sbjct: 1   MALQFSLQLFLLIASIAPHVITARVRHTHISSHLQGSRIGNNIEGIHNVKLYLKRYGYLT 60

Query: 61  NVKSTNPN-VFDDLLESAIKTFQKYHSLNVSGILDKETLALMSQPRCGIPDILHNNNITH 120
           NV+STN N  FD LLESAIKTFQKYHSLNVSG++D+ETL LMS PRCGIPDI+HN N+  
Sbjct: 61  NVESTNSNNTFDVLLESAIKTFQKYHSLNVSGVIDEETLTLMSLPRCGIPDIMHNINVMD 120

Query: 121 SGIQINSTTFHSHFSFFPGSPKWPISKYHLTYRFLDSFPNNFVAPVTNAMEQWGKFSKFT 180
           + +Q+NS++FHSHF+FFP + KWP+SKY+L Y FLD FPN+F  PV NAMEQW  FS F 
Sbjct: 121 NNVQMNSSSFHSHFTFFPDNLKWPVSKYNLRYTFLDDFPNDFKEPVMNAMEQWALFSLFR 180

Query: 181 FSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHAFGPPDGRVHFDADESWVDGSVSG 240
           FS A  +Q+ADITFNFVRGNHGDGYPF+GKGG LAHAFGP DGRVHFD DE W DGSV G
Sbjct: 181 FSEAVEAQEADITFNFVRGNHGDGYPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVGG 240

Query: 241 FFNVGMVALHELGHVLGLGHSTIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLYS 294
           F NVGMVALHELGHVLGL HSTIRDAIMWPYM  G+Q RGLQFDDI+GIQTLY+
Sbjct: 241 FINVGMVALHELGHVLGLAHSTIRDAIMWPYMEAGEQTRGLQFDDIEGIQTLYA 294

[Close]
BLAST of Lsi02G015330 vs. TrEMBL
Match: A0A0A0KS35_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632070 PE=3 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 2.0e-74
Identity = 157/305 (51.48%), Postives = 196/305 (64.26%), Query Frame = 1 

Query: 1   MALKFFLQLFLLFASVAPHMITARVLHTNISS------HLQGSRIGNN-TEGIHNVKLYL 60
           MA K  LQLFLL A V PH+ +AR LH + SS      HL GS   ++  EGIH VK YL
Sbjct: 1   MAAKLCLQLFLLLAFVTPHLTSARPLHNHKSSRLLFPEHLIGSSKNDSIVEGIHKVKAYL 60

Query: 61  QRYGYLTNVKSTN--PNVFDDLLESAIKTFQKYHSLNVSGILDKETLALMSQPRCGIPDI 120
           QRYGY +N    N   + FDD LESAIK++QK+ +L VSG+LD+ETL  MS+PRCG+ D 
Sbjct: 61  QRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVAD- 120

Query: 121 LHNNNITHSGIQINSTTFH---SHFSFFPGSPKWPISKYHLTYRFLDSFPNNFVAPVTNA 180
            +  ++     + N+TT     SHF FFPG  KWP  K+HLTY F+ ++P    A V  A
Sbjct: 121 -NFQSVAQQDGENNNTTVQIGGSHFMFFPGKGKWPYRKWHLTYGFVHNYPMKHAAAVVRA 180

Query: 181 MEQWGKFSKFTFSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHAFGPPDGRVHFDA 240
            ++W   SKFTFS+A R Q ADI  +F RG+HGDG PF+G+GG L HAFGP DGRVHFDA
Sbjct: 181 FDKWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAFGPIDGRVHFDA 240

Query: 241 DESWVD-GSVSG-FFNVGMVALHELGHVLGLGHSTIRDAIMWPYMNPGDQFRGLQFDDIQ 292
           DE W + GS++   F+   VALHE+GH LGLGHS    A+MW  M  G     L  DDI+
Sbjct: 241 DEQWAEIGSLTNENFDFESVALHEIGHALGLGHSIFPSAVMWANMETGVNKTELTIDDIE 300

[Close]
BLAST of Lsi02G015330 vs. TrEMBL
Match: B9RUG6_RICCO (Metalloendoproteinase 1, putative OS=Ricinus communis GN=RCOM_0852690 PE=3 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 6.8e-70
Identity = 141/301 (46.84%), Postives = 187/301 (62.13%), Query Frame = 1 

Query: 1   MALKFFLQLFLLFASVAPHMITARVLHTNISSHLQGSRIGNNTEGIHNVKLYLQRYGYLT 60
           + L   + L    A    H  T +    +   HLQG   G+N +GIH++K YL+ +GYL+
Sbjct: 11  VTLILLISLLSSAALAHSHSKTEKSSAFDFLKHLQGCHKGDNLKGIHDLKKYLENFGYLS 70

Query: 61  --NVKSTNPNVFDDLLESAIKTFQKYHSLNVSGILDKETLALMSQPRCGIPDILHNNNIT 120
             N   +N + FDDLLE A+KT+Q  + LNV+G LD ET+  M  PRCG+ DI++     
Sbjct: 71  YKNQSHSNDDDFDDLLEYALKTYQFNYHLNVTGFLDSETVTKMMMPRCGVADIINGTTRM 130

Query: 121 HSGIQI----NSTTFH--SHFSFFPGSPKWPISKYHLTYRFLDSFPNNFVAPVTNAMEQW 180
            S  +     +ST+FH  SH+ FFPG+P+WP SKYHLTY FL   PN  + PV  A + W
Sbjct: 131 QSSNKNPHHHSSTSFHTVSHYEFFPGNPRWPASKYHLTYGFLPGTPNQAMEPVAKAFQTW 190

Query: 181 GKFSKFTFSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHAFGPPDGRVHFDADESW 240
              + F F+     + ADIT  F RG+HGDG PF+G+GGTLAHAF P DGR H+D DE W
Sbjct: 191 AANTHFRFTRVQDYRAADITIGFHRGDHGDGSPFDGRGGTLAHAFAPQDGRFHYDGDEHW 250

Query: 241 VDGSVSGFFNVGMVALHELGHVLGLGHSTIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLY 294
             G+  G F+V  VALHE+GH+LGLGHS++  AIM P +  G   +GL  DDIQGI+ LY
Sbjct: 251 AVGATQGAFDVETVALHEIGHLLGLGHSSVEGAIMHPSIQSG-ATKGLHSDDIQGIRALY 310

[Close]
BLAST of Lsi02G015330 vs. TrEMBL
Match: A0A0A0M032_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G654840 PE=3 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 8.9e-70
Identity = 141/305 (46.23%), Postives = 193/305 (63.28%), Query Frame = 1 

Query: 11  LLFASVAPHMITA---RVLHTNISSHLQGSRIGNNTEGIHNVKLYLQRYGYLTN------ 70
           LLF S+ P   +    +V   +  + LQGS+ G+N +GI  +K + +RYGYL +      
Sbjct: 9   LLFISILPLCFSLPLRQVSRLSFLNDLQGSKKGDNVKGISKLKNFFRRYGYLNHQINVTG 68

Query: 71  --VKSTNPNVFDDLLESAIKTFQKYHSLNVSGILDKETLALMSQPRCGIPDILH------ 130
             +     + FDD  ESA+KT+Q+Y  LN +G L+ ETL+ ++ PRCG PDIL+      
Sbjct: 69  HLIDHDADDTFDDRFESAVKTYQQYFHLNSTGSLNAETLSQLATPRCGNPDILNEATGRM 128

Query: 131 -----NNNITHSGIQINSTTFHSHFSFFPGSPKWPISKYHLTYRFLDSFPNNFVAPVTNA 190
                NN+ +H      S     H+SFFPG P+WP +KYHLTY FL +   +  APVT A
Sbjct: 129 LLENNNNDSSHDHYHQLSHAV-PHYSFFPGRPRWPPTKYHLTYEFLPNTHADAKAPVTRA 188

Query: 191 MEQWGKFSKFTFSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHAFGPPDGRVHFDA 250
              W + + F FS+A  S++AD+   F RGNHGDGYPF+G GGTLAHAF P DGRVHFD+
Sbjct: 189 FATWARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAHAFTPTDGRVHFDS 248

Query: 251 DESWVDGSVSGFFNVGMVALHELGHVLGLGHSTIRDAIMWPYMNPGDQFRGLQFDDIQGI 294
            E WV G+V G F++  VALHE+GH+LGLGHS +++AIM+P +  G   +GL  DDI+GI
Sbjct: 249 TEKWVVGAVRGRFDLETVALHEIGHLLGLGHSRVKNAIMYPTIESGST-KGLNADDIEGI 308

[Close]
BLAST of Lsi02G015330 vs. TrEMBL
Match: A0A0A0KS39_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632120 PE=3 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 7.5e-69
Identity = 137/275 (49.82%), Postives = 176/275 (64.00%), Query Frame = 1 

Query: 32  SHLQGSRIGNNTEGIHNVKLYLQRYGYLTNV----KSTNPNVFDDLLESAIKTFQKYHSL 91
           + L GS+ G+  EGIH +K YL ++GYL          N + FD+LLE+AIKT+QK ++L
Sbjct: 44  NQLYGSQKGDKVEGIHQLKKYLHQFGYLNEDAQIHSENNDDEFDELLETAIKTYQKNYNL 103

Query: 92  NVSGILDKETLALMSQPRCGIPDILHNNNITHSGIQINSTT------FHS--HFSFFPGS 151
            V+G LD  T+A MS+PRCG+ DI+H N    S  +           FHS  HF+FF G+
Sbjct: 104 KVTGTLDAMTIAQMSKPRCGVADIIHGNTWMRSSKKRKQLEHGIVGHFHSVSHFAFFDGN 163

Query: 152 PKWPISKYHLTYRFLDSFPNNFVAPVTNAMEQWGKFSKFTFSVAARSQKADITFNFVRGN 211
           PKWP +K HLTY FL   P+  V+PV  A   W   S FTFS A  +Q +DIT  F  G+
Sbjct: 164 PKWPATKSHLTYGFLPGTPSETVSPVARAFATWAANSHFTFSQALDNQTSDITIGFETGD 223

Query: 212 HGDGYPFEGKGGTLAHAFGPPDGRVHFDADESWVDGSVSGFFNVGMVALHELGHVLGLGH 271
           HGDGYPF+G GG +AHAF P DGR H DADESW +G VS  F++  VALHE+GH+LGL H
Sbjct: 224 HGDGYPFDGVGGVVAHAFSPSDGRFHLDADESWANGVVSDSFDLETVALHEIGHLLGLQH 283

Query: 272 STIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLYSS 295
           S+   AIMWP + P    +GL  DDI GI+ LY++
Sbjct: 284 SSTEGAIMWPQV-PVGASKGLHVDDIAGIKALYNN 317

[Close]
BLAST of Lsi02G015330 vs. TAIR10
Match: AT1G70170.1 (AT1G70170.1 matrix metalloproteinase)

HSP 1 Score: 208.4 bits (529), Expect = 6.1e-54
Identity = 114/318 (35.85%), Postives = 178/318 (55.97%), Query Frame = 1 

Query: 6   FLQLFLLFA-----------SVAPHMITARVLHTNISSHLQGSRIGNNTEGIHNVKLYLQ 65
           FL LFL+ +           +V P +     +  +  S+  G   G N +G++ +K Y Q
Sbjct: 8   FLSLFLIVSPASAWFFPNSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKKYFQ 67

Query: 66  RYGYLTNVKSTN-PNVFDDLLESAIKTFQKYHSLNVSGILDKETLALMSQPRCGIPDILH 125
           R+GY+    S N  + FDD+L++A++ +Q   +LNV+G LD  T+  +  PRCG PD+++
Sbjct: 68  RFGYIPETFSGNFTDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNPDVVN 127

Query: 126 NNNITHSG----IQINSTTFHSH----FSFFPGSPKWPISKYHLTYRFLDSFP--NNFVA 185
             ++ H G     ++N +  H H    ++ FPG P+WP ++  LTY F    P      +
Sbjct: 128 GTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEVKS 187

Query: 186 PVTNAMEQWGKFSKFTFSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHAFGPPDGR 245
             + A  +W   +   F+++     +DIT  F  G+HGDG PF+G  GTLAHAF PP G+
Sbjct: 188 VFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGK 247

Query: 246 VHFDADESW-VDGSVSGFFNV------GMVALHELGHVLGLGHSTIRDAIMWPYMNPGDQ 295
            H DADE+W V G +  F +V        VA+HE+GH+LGLGHS++ ++IM+P +  G +
Sbjct: 248 FHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKR 307

[Close]
BLAST of Lsi02G015330 vs. TAIR10
Match: AT1G59970.1 (AT1G59970.1 Matrixin family protein)

HSP 1 Score: 207.6 bits (527), Expect = 1.0e-53
Identity = 112/274 (40.88%), Postives = 158/274 (57.66%), Query Frame = 1 

Query: 32  SHLQGSRIGNNTEGIHNVKLYLQRYGYLTNVKSTNPNVFDDLLESAIKTFQKYHSLNVSG 91
           S L G  IG N  G+  +K Y +R+GY+T   +   + FDD+L+SAI T+QK  +L V+G
Sbjct: 46  SKLAGCHIGENINGLSKLKQYFRRFGYITTTGNCTDD-FDDVLQSAINTYQKNFNLKVTG 105

Query: 92  ILDKETLALMSQPRCGIPDILHNNNITHSGIQINSTTFHSHFSFFPGSPKWPISKYHLTY 151
            LD  TL  + +PRCG PD++   +  + G  + +T     +SFFPG P+WP  K  LTY
Sbjct: 106 KLDSSTLRQIVKPRCGNPDLIDGVSEMNGGKILRTT---EKYSFFPGKPRWPKRKRDLTY 165

Query: 152 RFLDSFPNNFVAPV----TNAMEQWGKFSKFTFSVAARSQKADITFNFVRGNHGDGYPFE 211
            F     NN    V    + A  +W + +   F+ +    +ADI   F  G HGDG PF+
Sbjct: 166 AFAPQ--NNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFD 225

Query: 212 GKGGTLAHAFGPPDGRVHFDADESWVDGS---------VSGFFNVGMVALHELGHVLGLG 271
           G  GTLAHA  PP G +H D DE W+  +         V+   ++  VA+HE+GH+LGLG
Sbjct: 226 GAMGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLG 285

Query: 272 HSTIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLY 293
           HS++ DAIM+P ++ GD+   L  DDI+GIQ LY
Sbjct: 286 HSSVEDAIMFPAISGGDRKVELAKDDIEGIQHLY 313

[Close]
BLAST of Lsi02G015330 vs. TAIR10
Match: AT1G24140.1 (AT1G24140.1 Matrixin family protein)

HSP 1 Score: 203.4 bits (516), Expect = 2.0e-52
Identity = 104/280 (37.14%), Postives = 162/280 (57.86%), Query Frame = 1 

Query: 36  GSRIGNNTEGIHNVKLYLQRYGYLT--NVKSTNPNVFDDLLESAIKTFQKYHSLNVSGIL 95
           G   G   +G++ +K Y Q +GY+T  N+     + FDD+L++A++ +Q+   LNV+G+L
Sbjct: 51  GCHAGKKYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVL 110

Query: 96  DKETLALMSQPRCGIPDILHNNNITHSGIQINSTTFHS---------HFSFFPGSPKWPI 155
           D+ TL  +  PRCG PD+++  +  HSG +    +F           H+SFFPG P+WP 
Sbjct: 111 DELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPR 170

Query: 156 SKYHLTYRF--LDSFPNNFVAPVTNAMEQWGKFSKFTFSVAARSQKADITFNFVRGNHGD 215
           ++  LTY F   ++      +  + A  +W + +  TF+   R   +DI+  F  G HGD
Sbjct: 171 NRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGD 230

Query: 216 GYPFEGKGGTLAHAFGPPDGRVHFDADESWV------DG--SVSGFFNVGMVALHELGHV 275
           G PF+G   TLAHAF PP G  H D +E+W+      DG  SVS   ++  VA+HE+GH+
Sbjct: 231 GEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHL 290

Query: 276 LGLGHSTIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLYSS 295
           LGLGHS++  +IM+P +  G +   L  DD++G+Q LY +
Sbjct: 291 LGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYGA 330

[Close]
BLAST of Lsi02G015330 vs. TAIR10
Match: AT4G16640.1 (AT4G16640.1 Matrixin family protein)

HSP 1 Score: 179.9 bits (455), Expect = 2.3e-45
Identity = 105/272 (38.60%), Postives = 146/272 (53.68%), Query Frame = 1 

Query: 32  SHLQGSRIGNNTEGIHNVKLYLQRYGYLTNVKSTNPNVFDDLLESAIKTFQKYHSLNVSG 91
           S L   +IG++  G+  +K YL R+GY+ +      +VFD  LESAI  +Q+   L ++G
Sbjct: 56  SRLVDVQIGSHVSGVSELKRYLHRFGYVNDGSEIFSDVFDGPLESAISLYQENLGLPITG 115

Query: 92  ILDKETLALMSQPRCGIPDILHNNNITHSGIQINSTTFHSHFSFFPGSPKWPISKYHLTY 151
            LD  T+ LMS PRCG+ D       TH  I  +     +H+++F G PKW  ++  LTY
Sbjct: 116 RLDTSTVTLMSLPRCGVSD-------THMTINNDFLHTTAHYTYFNGKPKW--NRDTLTY 175

Query: 152 RF-----LDSFPNNFVAPV-TNAMEQWGKFSKFTFSVAARSQKADITFNFVRGNHGDGYP 211
                  LD   +  V  V   A  QW      +F        AD+   F  G+HGDG P
Sbjct: 176 AISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLP 235

Query: 212 FEGKGGTLAHAFGPPDGRVHFDADESW-VDGSVSGFFNVGM----VALHELGHVLGLGHS 271
           F+G  GTLAHAF P +GR+H DA E+W VD  + G   V +    VA HE+GH+LGLGHS
Sbjct: 236 FDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHS 295

Query: 272 TIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLY 293
           +   A+M+P + P  +   L  DD+ G+  LY
Sbjct: 296 SQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]
BLAST of Lsi02G015330 vs. TAIR10
Match: AT2G45040.1 (AT2G45040.1 Matrixin family protein)

HSP 1 Score: 157.1 bits (396), Expect = 1.6e-38
Identity = 91/262 (34.73%), Postives = 134/262 (51.15%), Query Frame = 1 

Query: 46  IHNVKLYLQRYGYLTNVKSTNPNVFDDLLESAIKTFQKYHSLNVSGILDKETLALMSQPR 105
           I  +K +LQ+YGYL   K ++    D   E A+  +QK   L ++G  D +TL+ +  PR
Sbjct: 50  IPEIKRHLQQYGYLPQNKESD----DVSFEQALVRYQKNLGLPITGKPDSDTLSQILLPR 109

Query: 106 CGIPDILHNNNITHSGIQINSTTFHS--HFSFFPGSPKWPIS-KYHLTYRFLDSFPNNFV 165
           CG PD           ++  +  FH+   + +FPG P+W       LTY F       ++
Sbjct: 110 CGFPD----------DVEPKTAPFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLTPYL 169

Query: 166 APVT------NAMEQWGKFSKFTFSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHA 225
           AP         A  +W      +F        ADI   F  G+HGDG PF+G  G LAH 
Sbjct: 170 APTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHT 229

Query: 226 FGPPDGRVHFDADESWV----DGSVSGFFNVGMVALHELGHVLGLGHSTIRDAIMWPYMN 285
           F P +GR+H D  E+W     +   S   ++  VA+HE+GHVLGLGHS+++DA M+P + 
Sbjct: 230 FSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLK 289

Query: 286 PGDQFRGLQFDDIQGIQTLYSS 295
           P  +   L  DD+ G+Q+LY +
Sbjct: 290 PRSKKVNLNMDDVVGVQSLYGT 297

[Close]
BLAST of Lsi02G015330 vs. NCBI nr
Match: gi|449449425|ref|XP_004142465.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 475.7 bits (1223), Expect = 5.8e-131
Identity = 227/294 (77.21%), Postives = 254/294 (86.39%), Query Frame = 1 

Query: 1   MALKFFLQLFLLFASVAPHMITARVLHTNISSHLQGSRIGNNTEGIHNVKLYLQRYGYLT 60
           MAL+F LQLFLL AS+APH+ITARV HT+ISSHLQGSRIGNN EGIHNVKLYL+RYGYLT
Sbjct: 1   MALQFSLQLFLLIASIAPHVITARVRHTHISSHLQGSRIGNNIEGIHNVKLYLKRYGYLT 60

Query: 61  NVKSTNPN-VFDDLLESAIKTFQKYHSLNVSGILDKETLALMSQPRCGIPDILHNNNITH 120
           NV+STN N  FD LLESAIKTFQKYHSLNVSG++D+ETL LMS PRCGIPDI+HN N+  
Sbjct: 61  NVESTNSNNTFDVLLESAIKTFQKYHSLNVSGVIDEETLTLMSLPRCGIPDIMHNINVMD 120

Query: 121 SGIQINSTTFHSHFSFFPGSPKWPISKYHLTYRFLDSFPNNFVAPVTNAMEQWGKFSKFT 180
           + +Q+NS++FHSHF+FFP + KWP+SKY+L Y FLD FPN+F  PV NAMEQW  FS F 
Sbjct: 121 NNVQMNSSSFHSHFTFFPDNLKWPVSKYNLRYTFLDDFPNDFKEPVMNAMEQWALFSLFR 180

Query: 181 FSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHAFGPPDGRVHFDADESWVDGSVSG 240
           FS A  +Q+ADITFNFVRGNHGDGYPF+GKGG LAHAFGP DGRVHFD DE W DGSV G
Sbjct: 181 FSEAVEAQEADITFNFVRGNHGDGYPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVGG 240

Query: 241 FFNVGMVALHELGHVLGLGHSTIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLYS 294
           F NVGMVALHELGHVLGL HSTIRDAIMWPYM  G+Q RGLQFDDI+GIQTLY+
Sbjct: 241 FINVGMVALHELGHVLGLAHSTIRDAIMWPYMEAGEQTRGLQFDDIEGIQTLYA 294

[Close]
BLAST of Lsi02G015330 vs. NCBI nr
Match: gi|659118023|ref|XP_008458910.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 453.4 bits (1165), Expect = 3.1e-124
Identity = 219/295 (74.24%), Postives = 250/295 (84.75%), Query Frame = 1 

Query: 1   MALKFFLQLFLLFASVAPHMITARVLHTNISSHLQGSRIGNNTEGIHNVKLYLQRYGYL- 60
           MAL+  LQLFLL ASVAPH+ITARV HT+I SHL+GSRIGNN EGI++VKLYL+RYGYL 
Sbjct: 1   MALQLSLQLFLLIASVAPHVITARVRHTHIPSHLKGSRIGNNIEGIYSVKLYLKRYGYLI 60

Query: 61  TNVKSTNPN-VFDDLLESAIKTFQKYHSLNVSGILDKETLALMSQPRCGIPDILHNNNIT 120
           TNV +T+ N  FD LLESAIKTFQKYHSLNVSG+LDKETL LMS PRCGI DI+HNNN+ 
Sbjct: 61  TNVPNTDSNDTFDVLLESAIKTFQKYHSLNVSGVLDKETLTLMSLPRCGISDIVHNNNVM 120

Query: 121 HSGIQINSTTFHSHFSFFPGSPKWPISKYHLTYRFLDSFPNNFVAPVTNAMEQWGKFSKF 180
            + IQ+NS++FHSH++F PG+PKWPISKY+L Y FL+ FPN+F  PV NAM+QW  FS F
Sbjct: 121 DNNIQMNSSSFHSHYTFLPGNPKWPISKYNLKYTFLEGFPNDFKEPVMNAMDQWALFSLF 180

Query: 181 TFSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHAFGPPDGRVHFDADESWVDGSVS 240
            FS     ++ DITFNFVRGNHGDG+PF+GKGG LAHAFGP DGRVHFD DE W DGSV 
Sbjct: 181 RFSETIGGEEVDITFNFVRGNHGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVG 240

Query: 241 GFFNVGMVALHELGHVLGLGHSTIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLYS 294
           GF NVGMVALHELGHVLGL HSTIRDAIMWPYM+ G+Q RGLQFDDI+GIQTLY+
Sbjct: 241 GFINVGMVALHELGHVLGLAHSTIRDAIMWPYMHVGEQTRGLQFDDIEGIQTLYA 295

[Close]
BLAST of Lsi02G015330 vs. NCBI nr
Match: gi|659118025|ref|XP_008458911.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 288.5 bits (737), Expect = 1.3e-74
Identity = 159/308 (51.62%), Postives = 193/308 (62.66%), Query Frame = 1 

Query: 1   MALKFFLQLFLLFASVAPHMITARVLHTNISS------HLQGSRIGNNTEGIHNVKLYLQ 60
           M  K  LQLFLLFA + PH+ +AR L  +  S      HL GSR  +N EGIH VK YLQ
Sbjct: 1   MGSKLCLQLFLLFAFITPHLTSARHLRNHKPSRLLFPEHLIGSRKNDNFEGIHKVKAYLQ 60

Query: 61  RYGYLTNVKSTNPNV--FDDLLESAIKTFQKYHSLNVSGILDKETLALMSQPRCGIPDIL 120
           RYGYL++ K  N ++  FDD LESAIK++QK   LNVSG+LD+ETL  M++PRCG+ D  
Sbjct: 61  RYGYLSDEKENNLSIDAFDDDLESAIKSYQKVFKLNVSGVLDRETLQQMARPRCGVTDDF 120

Query: 121 -------HNNNITHSGIQINSTTFHSHFSFFPGSPKWPISKYHLTYRFLDSFPNNFVAPV 180
                  H NN T+  IQI      SH+ F+P   KWP+ K HLTY F+ ++P    A V
Sbjct: 121 ILVAQQDHENNNTN--IQIGG----SHYMFYPNKLKWPVEKRHLTYGFIHNYPPEHAAAV 180

Query: 181 TNAMEQWGKFSKFTFSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHAFGPPDGRVH 240
             A + W   +KFTFS AAR Q ADI  +F RG+HGDG PF+G GG L HAFG  DGRVH
Sbjct: 181 IRAFDTWEANTKFTFSHAARVQTADILLSFERGDHGDGKPFDGPGGALGHAFGAIDGRVH 240

Query: 241 FDADESWVDGSV-SGFFNVGMVALHELGHVLGLGHSTIRDAIMWPYMNPGDQFRGLQFDD 293
           FD DE W  GS    +F+V  VALHE+GH LGLGHS    A+MW  M+ G     L  DD
Sbjct: 241 FDGDEQWETGSFWQEYFDVESVALHEIGHALGLGHSREPSAVMWANMDSGVTKTHLTIDD 300

[Close]
BLAST of Lsi02G015330 vs. NCBI nr
Match: gi|778708735|ref|XP_004142466.2| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 287.3 bits (734), Expect = 2.9e-74
Identity = 157/305 (51.48%), Postives = 196/305 (64.26%), Query Frame = 1 

Query: 1   MALKFFLQLFLLFASVAPHMITARVLHTNISS------HLQGSRIGNN-TEGIHNVKLYL 60
           MA K  LQLFLL A V PH+ +AR LH + SS      HL GS   ++  EGIH VK YL
Sbjct: 1   MAAKLCLQLFLLLAFVTPHLTSARPLHNHKSSRLLFPEHLIGSSKNDSIVEGIHKVKAYL 60

Query: 61  QRYGYLTNVKSTN--PNVFDDLLESAIKTFQKYHSLNVSGILDKETLALMSQPRCGIPDI 120
           QRYGY +N    N   + FDD LESAIK++QK+ +L VSG+LD+ETL  MS+PRCG+ D 
Sbjct: 61  QRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVAD- 120

Query: 121 LHNNNITHSGIQINSTTFH---SHFSFFPGSPKWPISKYHLTYRFLDSFPNNFVAPVTNA 180
            +  ++     + N+TT     SHF FFPG  KWP  K+HLTY F+ ++P    A V  A
Sbjct: 121 -NFQSVAQQDGENNNTTVQIGGSHFMFFPGKGKWPYRKWHLTYGFVHNYPMKHAAAVVRA 180

Query: 181 MEQWGKFSKFTFSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHAFGPPDGRVHFDA 240
            ++W   SKFTFS+A R Q ADI  +F RG+HGDG PF+G+GG L HAFGP DGRVHFDA
Sbjct: 181 FDKWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAFGPIDGRVHFDA 240

Query: 241 DESWVD-GSVSG-FFNVGMVALHELGHVLGLGHSTIRDAIMWPYMNPGDQFRGLQFDDIQ 292
           DE W + GS++   F+   VALHE+GH LGLGHS    A+MW  M  G     L  DDI+
Sbjct: 241 DEQWAEIGSLTNENFDFESVALHEIGHALGLGHSIFPSAVMWANMETGVNKTELTIDDIE 300

[Close]
BLAST of Lsi02G015330 vs. NCBI nr
Match: gi|659118035|ref|XP_008458917.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 280.4 bits (716), Expect = 3.6e-72
Identity = 147/301 (48.84%), Postives = 193/301 (64.12%), Query Frame = 1 

Query: 1   MALKFFLQLFLLFASVAPHMITARVLHTNISSHLQGSRIGNNTEGIHNVKLYLQRYGYLT 60
           MA K     FLL AS   H+I+ R + T I  HL G R G+N EGIH++K YL RYGYL+
Sbjct: 1   MAFKSLQLFFLLLASFTSHVIS-RHIPTPIPQHLYGCRKGDNVEGIHSIKKYLHRYGYLS 60

Query: 61  NVKST------NPNVFDDLLESAIKTFQKYHSLNVSGILDKETLALMSQPRCGIPDIL-- 120
           +  +T      N N FDD LESA+K +QK+  LNVSGILD++TL  + +PRCG+PD++  
Sbjct: 61  HNTNTSSHIELNSNTFDDTLESAVKLYQKWSKLNVSGILDQQTLDQIFKPRCGLPDVIMK 120

Query: 121 -HNNNITHSGIQINSTTFHSHFSFFPGSPKWPISKYHLTYRFLDSFPNNFVAPVTNAMEQ 180
            ++N  T   ++I+S     H++ FPG+ KWP  K HLTY F ++FP +FV  VT AM++
Sbjct: 121 SNSNKNTEDDLEISS-----HYALFPGNQKWPDYKRHLTYTFTNNFPIDFVPSVTEAMKE 180

Query: 181 WGKFSKFTFSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHAFGPPDGRVHFDADES 240
           W   S FTFS AA  Q  DI  +F  G+HGDG PF+G GG + HAF P DGR+H D D+ 
Sbjct: 181 WAAHSLFTFSQAADDQVPDINISFQVGDHGDGVPFDGPGGVVGHAFAPTDGRLHLDGDDK 240

Query: 241 WVDGSVSGFFNVGMVALHELGHVLGLGHSTIRDAIMWPYMNPGDQFRGLQFDDIQGIQTL 293
           W  G  +   NV   ALHELGHVLGL HST++ A+MWPY++     + L  DDI G+  L
Sbjct: 241 WSAGLETQKVNVMNAALHELGHVLGLAHSTVQQAVMWPYIDV-YALKALTDDDIAGLTAL 294

The following BLAST results are available for this feature:
BLAST of Lsi02G015330 vs. Swiss-Prot
Match NameE-valueIdentityDescription
2MMP_ARATH1.1e-5235.85Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1[more]
5MMP_ARATH1.8e-5240.88Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1[more]
3MMP_ARATH3.5e-5137.14Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1[more]
MEP1_SOYBN1.9e-4942.15Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2[more]
1MMP_ARATH4.1e-4438.60Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1[more]
BLAST of Lsi02G015330 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KU02_CUCSA4.1e-13177.21Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632080 PE=3 SV=1[more]
A0A0A0KS35_CUCSA2.0e-7451.48Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632070 PE=3 SV=1[more]
B9RUG6_RICCO6.8e-7046.84Metalloendoproteinase 1, putative OS=Ricinus communis GN=RCOM_0852690 PE=3 SV=1[more]
A0A0A0M032_CUCSA8.9e-7046.23Uncharacterized protein OS=Cucumis sativus GN=Csa_1G654840 PE=3 SV=1[more]
A0A0A0KS39_CUCSA7.5e-6949.82Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632120 PE=3 SV=1[more]
BLAST of Lsi02G015330 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G70170.16.1e-5435.85 matrix metalloproteinase[more]
AT1G59970.11.0e-5340.88 Matrixin family protein[more]
AT1G24140.12.0e-5237.14 Matrixin family protein[more]
AT4G16640.12.3e-4538.60 Matrixin family protein[more]
AT2G45040.11.6e-3834.73 Matrixin family protein[more]
BLAST of Lsi02G015330 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449449425|ref|XP_004142465.1|5.8e-13177.21PREDICTED: metalloendoproteinase 1-like [Cucumis sativus][more]
gi|659118023|ref|XP_008458910.1|3.1e-12474.24PREDICTED: metalloendoproteinase 1-like [Cucumis melo][more]
gi|659118025|ref|XP_008458911.1|1.3e-7451.62PREDICTED: metalloendoproteinase 1-like [Cucumis melo][more]
gi|778708735|ref|XP_004142466.2|2.9e-7451.48PREDICTED: metalloendoproteinase 1-like [Cucumis sativus][more]
gi|659118035|ref|XP_008458917.1|3.6e-7248.84PREDICTED: metalloendoproteinase 1-like [Cucumis melo][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0008237metallopeptidase activity
GO:0008270zinc ion binding
GO:0004222metalloendopeptidase activity
Vocabulary: Cellular Component
TermDefinition
GO:0031012extracellular matrix
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: INTERPRO
TermDefinition
IPR024079MetalloPept_cat_dom_sf
IPR021190Pept_M10A
IPR021158Pept_M10A_Zn_BS
IPR006026Peptidase_Metallo
IPR002477Peptidoglycan-bd-like
IPR001818Pept_M10_metallopeptidase
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0031012 extracellular matrix
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0008237 metallopeptidase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G015330.1Lsi02G015330.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001818Peptidase M10, metallopeptidasePFAMPF00413Peptidase_M10coord: 146..292
score: 8.8
IPR002477Peptidoglycan binding-likeGENE3DG3DSA:1.10.101.10coord: 46..102
score: 8.4
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 44..101
score: 3.3
IPR002477Peptidoglycan binding-likeunknownSSF47090PGBD-likecoord: 38..111
score: 6.28
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 138..294
score: 3.2
IPR021158Peptidase M10A, cysteine switch, zinc binding sitePROSITEPS00546CYSTEINE_SWITCHcoord: 104..111
scor
IPR021190Peptidase M10APRINTSPR00138MATRIXINcoord: 246..271
score: 6.5E-36coord: 101..114
score: 6.5E-36coord: 165..180
score: 6.5E-36coord: 280..293
score: 6.5E-36coord: 189..217
score: 6.5
IPR024079Metallopeptidase, catalytic domainGENE3DG3DSA:3.40.390.10coord: 103..293
score: 1.6
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 1..292
score: 1.8E
NoneNo IPR availablePANTHERPTHR10201:SF152SUBFAMILY NOT NAMEDcoord: 1..292
score: 1.8E
NoneNo IPR availableunknownSSF55486Metalloproteases ("zincins"), catalytic domaincoord: 135..292
score: 1.54

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Lsi02G015330Csa5G631560Cucumber (Chinese Long) v2culsiB348
Lsi02G015330CSPI05G28430Wild cucumber (PI 183967)cpilsiB352
Lsi02G015330Cucsa.158800Cucumber (Gy14) v1cgylsiB251
Lsi02G015330MELO3C021501.2Melon (DHL92) v3.6.1lsimedB171
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi02G015330Wax gourdlsiwgoB207
Lsi02G015330Wax gourdlsiwgoB253
Lsi02G015330Bottle gourd (USVL1VR-Ls)lsilsiB089
Lsi02G015330Cucumber (Gy14) v1cgylsiB118
Lsi02G015330Cucurbita maxima (Rimu)cmalsiB272
Lsi02G015330Cucurbita maxima (Rimu)cmalsiB372
Lsi02G015330Cucurbita maxima (Rimu)cmalsiB651
Lsi02G015330Cucurbita moschata (Rifu)cmolsiB261
Lsi02G015330Cucurbita moschata (Rifu)cmolsiB640
Lsi02G015330Cucurbita pepo (Zucchini)cpelsiB141
Lsi02G015330Cucurbita pepo (Zucchini)cpelsiB336
Lsi02G015330Wild cucumber (PI 183967)cpilsiB028
Lsi02G015330Cucumber (Chinese Long) v2culsiB028
Lsi02G015330Melon (DHL92) v3.5.1lsimeB162
Lsi02G015330Melon (DHL92) v3.5.1lsimeB182
Lsi02G015330Watermelon (Charleston Gray)lsiwcgB155
Lsi02G015330Watermelon (Charleston Gray)lsiwcgB176
Lsi02G015330Watermelon (97103) v1lsiwmB170
Lsi02G015330Cucumber (Gy14) v2cgyblsiB024
Lsi02G015330Cucumber (Gy14) v2cgyblsiB319
Lsi02G015330Melon (DHL92) v3.6.1lsimedB191
Lsi02G015330Silver-seed gourdcarlsiB232
Lsi02G015330Silver-seed gourdcarlsiB623
Lsi02G015330Cucumber (Chinese Long) v3cuclsiB028
Lsi02G015330Cucumber (Chinese Long) v3cuclsiB371
Lsi02G015330Watermelon (97103) v2lsiwmbB161
Lsi02G015330Watermelon (97103) v2lsiwmbB208