Lsi02G015320 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi02G015320
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionMatrix metalloproteinase
Locationchr02 : 21195745 .. 21196686 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTTCAAATCATCATCTCTCCAACTCTTCCTACTTTTTCTTGCTTCCATAGCATTTCACCCGATTTCGGCACGTGATCTCGATGACATCCATAAATCATCTCAATTTCTATTTCCTCAACATCTTTTAGGAAGTCGTAAAGGTCACAACATCGAAGGAATCCATAGCATCAAAACACACCTCCAACGTTACGGTTACTTAAACAAAAACTATAACATTATCGATACCAATAGCGCTTATAATAACGCCTTCGACGACCACCTAGAATCCGCGGTAAAAAAATACCAAAAATTCTTCAAGCTTAACGAGAGCGGAATTTTAGACGTGGAGACGTTGCACCAAATGTCACAGGCTCGTTGTTCGATTCCCGACATATTCGAGAACGGCGACAACGAGACGAGTGTGACGACAAGTAATCTCCATATAGGAAGTAAGTACACATTTTTCCCAGGGAGAGTAAAATGGCCGAGCTGGAAGAAATACCAACTAACATACTCATTCCTTCGGAATTTCCCAGAAGGGTTTAAGGAGTCAGTGCGTACGGCGTTTATGATATGGTATGAACGCAGCCGATTTAATTTCACAGAAGTTGATGAGAATAAAGGGGCAGATATAAGAATAAGCTTTGAGGTAGGAAACCATGGAGATTGGTATCCTTTCAAGAAGGGATCAGGAGTTTTGGCACATACGTTTGGGCCTGGAGATGGGAGATTTCACTTCAATGCTGATCAACCTTTTTCAGTTGAAGTTACATATGGTATGTATCATTTGAGGACTATTGCATTGCATGAGCTTGGACATGCACTTGGGTTGGCTCACAGCACCAATGAAAACGCCATCATGTTTCCCACTGTACCTCCTAATGTTGTTAGGGATTTAGATATGGATGATGTTAATGGATTGTGGGACTTATATGATGGATTTGATGATGCTGATTAA

mRNA sequence

ATGGATTTCAAATCATCATCTCTCCAACTCTTCCTACTTTTTCTTGCTTCCATAGCATTTCACCCGATTTCGGCACGTGATCTCGATGACATCCATAAATCATCTCAATTTCTATTTCCTCAACATCTTTTAGGAAGTCGTAAAGGTCACAACATCGAAGGAATCCATAGCATCAAAACACACCTCCAACGTTACGGTTACTTAAACAAAAACTATAACATTATCGATACCAATAGCGCTTATAATAACGCCTTCGACGACCACCTAGAATCCGCGGTAAAAAAATACCAAAAATTCTTCAAGCTTAACGAGAGCGGAATTTTAGACGTGGAGACGTTGCACCAAATGTCACAGGCTCGTTGTTCGATTCCCGACATATTCGAGAACGGCGACAACGAGACGAGTGTGACGACAAGTAATCTCCATATAGGAAGTAAGTACACATTTTTCCCAGGGAGAGTAAAATGGCCGAGCTGGAAGAAATACCAACTAACATACTCATTCCTTCGGAATTTCCCAGAAGGGTTTAAGGAGTCAGTGCGTACGGCGTTTATGATATGGTATGAACGCAGCCGATTTAATTTCACAGAAGTTGATGAGAATAAAGGGGCAGATATAAGAATAAGCTTTGAGGTAGGAAACCATGGAGATTGGTATCCTTTCAAGAAGGGATCAGGAGTTTTGGCACATACGTTTGGGCCTGGAGATGGGAGATTTCACTTCAATGCTGATCAACCTTTTTCAGTTGAAGTTACATATGGTATGTATCATTTGAGGACTATTGCATTGCATGAGCTTGGACATGCACTTGGGTTGGCTCACAGCACCAATGAAAACGCCATCATGTTTCCCACTGTACCTCCTAATGTTGTTAGGGATTTAGATATGGATGATGTTAATGGATTGTGGGACTTATATGATGGATTTGATGATGCTGATTAA

Coding sequence (CDS)

ATGGATTTCAAATCATCATCTCTCCAACTCTTCCTACTTTTTCTTGCTTCCATAGCATTTCACCCGATTTCGGCACGTGATCTCGATGACATCCATAAATCATCTCAATTTCTATTTCCTCAACATCTTTTAGGAAGTCGTAAAGGTCACAACATCGAAGGAATCCATAGCATCAAAACACACCTCCAACGTTACGGTTACTTAAACAAAAACTATAACATTATCGATACCAATAGCGCTTATAATAACGCCTTCGACGACCACCTAGAATCCGCGGTAAAAAAATACCAAAAATTCTTCAAGCTTAACGAGAGCGGAATTTTAGACGTGGAGACGTTGCACCAAATGTCACAGGCTCGTTGTTCGATTCCCGACATATTCGAGAACGGCGACAACGAGACGAGTGTGACGACAAGTAATCTCCATATAGGAAGTAAGTACACATTTTTCCCAGGGAGAGTAAAATGGCCGAGCTGGAAGAAATACCAACTAACATACTCATTCCTTCGGAATTTCCCAGAAGGGTTTAAGGAGTCAGTGCGTACGGCGTTTATGATATGGTATGAACGCAGCCGATTTAATTTCACAGAAGTTGATGAGAATAAAGGGGCAGATATAAGAATAAGCTTTGAGGTAGGAAACCATGGAGATTGGTATCCTTTCAAGAAGGGATCAGGAGTTTTGGCACATACGTTTGGGCCTGGAGATGGGAGATTTCACTTCAATGCTGATCAACCTTTTTCAGTTGAAGTTACATATGGTATGTATCATTTGAGGACTATTGCATTGCATGAGCTTGGACATGCACTTGGGTTGGCTCACAGCACCAATGAAAACGCCATCATGTTTCCCACTGTACCTCCTAATGTTGTTAGGGATTTAGATATGGATGATGTTAATGGATTGTGGGACTTATATGATGGATTTGATGATGCTGATTAA

Protein sequence

MDFKSSSLQLFLLFLASIAFHPISARDLDDIHKSSQFLFPQHLLGSRKGHNIEGIHSIKTHLQRYGYLNKNYNIIDTNSAYNNAFDDHLESAVKKYQKFFKLNESGILDVETLHQMSQARCSIPDIFENGDNETSVTTSNLHIGSKYTFFPGRVKWPSWKKYQLTYSFLRNFPEGFKESVRTAFMIWYERSRFNFTEVDENKGADIRISFEVGNHGDWYPFKKGSGVLAHTFGPGDGRFHFNADQPFSVEVTYGMYHLRTIALHELGHALGLAHSTNENAIMFPTVPPNVVRDLDMDDVNGLWDLYDGFDDAD
Homology
[Close]
BLAST of Lsi02G015320 vs. Swiss-Prot
Match: MEP1_SOYBN (Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2)

HSP 1 Score: 167.5 bits (423), Expect = 2.3e-40
Identity = 99/267 (37.08%), Postives = 144/267 (53.93%), Query Frame = 1 

Query: 49  GHNIEGIHSIKTHLQRYGYLNKNYNIIDTNSAYNNAFDDHLESAVKKYQKFFKLNESGIL 108
           G N +G+ ++K +    GY       I     +++ FDD L SA+K YQK + LN +G  
Sbjct: 48  GQNYKGLSNVKNYFHHLGY-------IPNAPHFDDNFDDTLVSAIKTYQKNYNLNVTGKF 107

Query: 109 DVETLHQMSQARCSIPDIFENGDNETSVTTSNLHIGSKYTFFPGRVKWPSWKKYQLTYSF 168
           D+ TL Q+   RC +PDI  N +  TS       + S YTFF    +W +    QLTY+F
Sbjct: 108 DINTLKQIMTPRCGVPDIIINTNKTTS-----FGMISDYTFFKDMPRWQAGTT-QLTYAF 167

Query: 169 LRN--FPEGFKESVRTAFMIWYERSRFNFTEVDENKGADIRISFEVGNHGDWYPFKKGSG 228
                  + FK ++  AF  W       F E    + A+I+I F   NHGD YPF    G
Sbjct: 168 SPEPRLDDTFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGG 227

Query: 229 VLAHTFGPGDGRFHFNADQPF--SVEVT----YGMYHLRTIALHELGHALGLAHSTNENA 288
           +L H F P DGR HF+AD+ +  S +VT       + L ++A+HE+GH LGL HS++  A
Sbjct: 228 ILGHAFAPTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRA 287

Query: 289 IMFPTVPPNVVR-DLDMDDVNGLWDLY 307
           IM+P++PP   + +L  DD++G+  LY
Sbjct: 288 IMYPSIPPRTRKVNLAQDDIDGIRKLY 301

[Close]
BLAST of Lsi02G015320 vs. Swiss-Prot
Match: 2MMP_ARATH (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 2.9e-40
Identity = 109/322 (33.85%), Postives = 165/322 (51.24%), Query Frame = 1 

Query: 8   LQLFLLFL-ASIAFHPISARDLDDIHKSSQFLFP--QHLLGSRKGHNIEGIHSIKTHLQR 67
           L LFL+   AS  F P S      +  +++  +    +  G   G N++G++ IK + QR
Sbjct: 9   LSLFLIVSPASAWFFPNSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKKYFQR 68

Query: 68  YGYLNKNYNIIDTNSAYNNAFDDHLESAVKKYQKFFKLNESGILDVETLHQMSQARCSIP 127
           +GY+ + ++       + + FDD L++AV+ YQ  F LN +G LD  T+  +   RC  P
Sbjct: 69  FGYIPETFS-----GNFTDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNP 128

Query: 128 DIFENGDN----------ETSVTTSNLHIGSKYTFFPGRVKWPSWKKYQLTYSFLRNFP- 187
           D+  NG +          E + + ++LH   +YT FPG  +WP  ++  LTY+F    P 
Sbjct: 129 DVV-NGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRR-DLTYAFDPKNPL 188

Query: 188 -EGFKESVRTAFMIWYERSRFNFTEVDENKGADIRISFEVGNHGDWYPFKKGSGVLAHTF 247
            E  K     AF  W + +  NFT  +    +DI I F  G+HGD  PF    G LAH F
Sbjct: 189 TEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAF 248

Query: 248 GPGDGRFHFNADQPFSVEVTYGMY-------HLRTIALHELGHALGLAHSTNENAIMFPT 307
            P  G+FH +AD+ + V      +        L ++A+HE+GH LGL HS+ E +IM+PT
Sbjct: 249 SPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPT 308

[Close]
BLAST of Lsi02G015320 vs. Swiss-Prot
Match: 1MMP_ARATH (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 1.8e-37
Identity = 96/272 (35.29%), Postives = 143/272 (52.57%), Query Frame = 1 

Query: 49  GHNIEGIHSIKTHLQRYGYLNKNYNIIDTNSAYNNAFDDHLESAVKKYQKFFKLNESGIL 108
           G ++ G+  +K +L R+GY+N      D +  +++ FD  LESA+  YQ+   L  +G L
Sbjct: 64  GSHVSGVSELKRYLHRFGYVN------DGSEIFSDVFDGPLESAISLYQENLGLPITGRL 123

Query: 109 DVETLHQMSQARCSIPDIFENGDNETSVTTSN--LHIGSKYTFFPGRVKWPSWKKYQLTY 168
           D  T+  MS  RC + D        T +T +N  LH  + YT+F G+   P W +  LTY
Sbjct: 124 DTSTVTLMSLPRCGVSD--------THMTINNDFLHTTAHYTYFNGK---PKWNRDTLTY 183

Query: 169 SFLRNFP------EGFKESVRTAFMIWYERSRFNFTEVDENKGADIRISFEVGNHGDWYP 228
           +  +         E  K   R AF  W      +F EVD+   AD++I F  G+HGD  P
Sbjct: 184 AISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLP 243

Query: 229 FKKGSGVLAHTFGPGDGRFHFNADQPFSVE-----VTYGMYHLRTIALHELGHALGLAHS 288
           F    G LAH F P +GR H +A + + V+      +     L ++A HE+GH LGL HS
Sbjct: 244 FDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHS 303

Query: 289 TNENAIMFPTVPPNVVR-DLDMDDVNGLWDLY 307
           + E+A+M+P++ P   + DL +DDV G+  LY
Sbjct: 304 SQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]
BLAST of Lsi02G015320 vs. Swiss-Prot
Match: 3MMP_ARATH (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 4.4e-36
Identity = 111/340 (32.65%), Postives = 162/340 (47.65%), Query Frame = 1 

Query: 10  LFLLFLASIAFHPISARDLDDIHKSSQFLFPQHL--------------LGSRKGHNIEGI 69
           +F++FL   A  P+SA      + +S  + PQ L               G   G   +G+
Sbjct: 6   VFMVFLLFFAPSPVSA----GFYTNSSAIPPQLLRNATGNPWNSFLNFTGCHAGKKYDGL 65

Query: 70  HSIKTHLQRYGYLNKNYNIIDTNSAYNNAFDDHLESAVKKYQKFFKLNESGILDVETLHQ 129
           + +K + Q +GY+ +     + +  + + FDD L++AV+ YQ+ F+LN +G+LD  TL  
Sbjct: 66  YMLKQYFQHFGYITET----NLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVLDELTLKH 125

Query: 130 MSQARCSIPDIFENGDNETSVTTSNLHIGSK------------------YTFFPGRVKWP 189
           +   RC  PD+  NG       TS +H G K                  Y+FFPG  +WP
Sbjct: 126 VVIPRCGNPDVV-NG-------TSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWP 185

Query: 190 SWKKYQLTYSF-LRN-FPEGFKESVRTAFMIWYERSRFNFTEVDENKGADIRISFEVGNH 249
             ++  LTY+F  RN   E  K     AF  W E +   FT V+    +DI I F  G H
Sbjct: 186 RNRR-DLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEH 245

Query: 250 GDWYPFKKGSGVLAHTFGPGDGRFHFNADQPFSVEVTYG--------MYHLRTIALHELG 307
           GD  PF      LAH F P  G FH + ++ + V    G           L ++A+HE+G
Sbjct: 246 GDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIG 305

[Close]
BLAST of Lsi02G015320 vs. Swiss-Prot
Match: 4MMP_ARATH (Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana GN=4MMP PE=1 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 4.4e-36
Identity = 96/263 (36.50%), Postives = 136/263 (51.71%), Query Frame = 1 

Query: 55  IHSIKTHLQRYGYLNKNYNIIDTNSAYNNAFDDHLESAVKKYQKFFKLNESGILDVETLH 114
           I  IK HLQ+YGYL +N    D +           E A+ +YQK   L  +G  D +TL 
Sbjct: 50  IPEIKRHLQQYGYLPQNKESDDVS----------FEQALVRYQKNLGLPITGKPDSDTLS 109

Query: 115 QMSQARCSIPDIFENGDNETSVTTSNLHIGSKYTFFPGRVKWPSWKKYQLTYSFLRN--- 174
           Q+   RC  PD       +    T+  H G KY +FPGR +W      +LTY+F +    
Sbjct: 110 QILLPRCGFPD-------DVEPKTAPFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLT 169

Query: 175 ---FPEGFKESVRTAFMIWYERSRFNFTEVDENKGADIRISFEVGNHGDWYPFKKGSGVL 234
               P   +   R AF  W      +F E ++   ADI+I F  G+HGD  PF    GVL
Sbjct: 170 PYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVL 229

Query: 235 AHTFGPGDGRFHFNADQPFSV----EVTYGMYHLRTIALHELGHALGLAHSTNENAIMFP 294
           AHTF P +GR H +  + ++V    E +     L ++A+HE+GH LGL HS+ ++A M+P
Sbjct: 230 AHTFSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYP 289

Query: 295 TVPPNVVR-DLDMDDVNGLWDLY 307
           T+ P   + +L+MDDV G+  LY
Sbjct: 290 TLKPRSKKVNLNMDDVVGVQSLY 295

[Close]
BLAST of Lsi02G015320 vs. TrEMBL
Match: A0A0A0KS15_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632090 PE=3 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 1.8e-137
Identity = 243/311 (78.14%), Postives = 269/311 (86.50%), Query Frame = 1 

Query: 1   MDFKSSS-LQLFLLFL-ASIAFHPISARDLDDIHKSSQFLFPQHLLGSRKGHNIEGIHSI 60
           MDFKSSS LQ+FLL L ASIAF  I ARDL DI K SQ LFPQHLLGS KGHNIEGIH+I
Sbjct: 1   MDFKSSSDLQIFLLLLLASIAFPFIWARDLHDI-KKSQLLFPQHLLGSSKGHNIEGIHTI 60

Query: 61  KTHLQRYGYLNKNYNIIDTNSAYNNAFDDHLESAVKKYQKFFKLNESGILDVETLHQMSQ 120
           K HLQRYGYL+KNYNIIDTN AYNNAFDDHLESA+KKYQ FFKL +SG+LD+ETLHQMSQ
Sbjct: 61  KMHLQRYGYLSKNYNIIDTNGAYNNAFDDHLESAIKKYQMFFKLPKSGVLDMETLHQMSQ 120

Query: 121 ARCSIPDIFENGDNETSVTTSNLHIGSKYTFFPGRVKWPSWKKYQLTYSFLRNFPEGFKE 180
           ARCS+PDIFEN +NETSVTTSNLHIGSKYTFFPGRVKWP    Y+LTY+ + NFPE FKE
Sbjct: 121 ARCSVPDIFENNENETSVTTSNLHIGSKYTFFPGRVKWPDSLNYRLTYALVNNFPEEFKE 180

Query: 181 SVRTAFMIWYERSRFNFTEVDENKGADIRISFEVGNHGDWYPFKKGSGVLAHTFGPGDGR 240
           SVRTAF IWY RSRFNFTEV EN+G +IRISFE G HGD++PF K S  LAHTF P DGR
Sbjct: 181 SVRTAFEIWYGRSRFNFTEVSENEGGNIRISFERGVHGDYHPFTKNSKTLAHTFAPIDGR 240

Query: 241 FHFNADQPFSVEVTYGMYHLRTIALHELGHALGLAHSTNENAIMFPTVPPNVVRDLDMDD 300
           FHFNAD+PFSV+VTY  YHLRT+ALHELGHA GLAHS +E+AIMFPT+P N+ +DLDMDD
Sbjct: 241 FHFNADKPFSVDVTYNAYHLRTVALHELGHAFGLAHSPSEDAIMFPTIPTNLEKDLDMDD 300

Query: 301 VNGLWDLYDGF 310
           + GLW+LYDGF
Sbjct: 301 IEGLWELYDGF 310

[Close]
BLAST of Lsi02G015320 vs. TrEMBL
Match: A0A0A0KX75_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632100 PE=3 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 4.5e-120
Identity = 207/310 (66.77%), Postives = 252/310 (81.29%), Query Frame = 1 

Query: 1   MDFKSSSLQLFLLFLASIA-FHPISARDLDDIHKSSQFLFPQHLLGSRKGHNIEGIHSIK 60
           MDFKS  LQLFLL L  +  FHPIS+RDL+ IH  SQFLFP++L+GSRKGHN+EG H+IK
Sbjct: 1   MDFKSLHLQLFLLLLTFLLLFHPISSRDLNHIHGPSQFLFPKYLIGSRKGHNLEGTHTIK 60

Query: 61  THLQRYGYLNKNYNIIDTNSAYNNAFDDHLESAVKKYQKFFKLNESGILDVETLHQMSQA 120
           T+LQRYGYL+K+YNIIDTN  YNNA+D+ LES++KKYQKFF+LN+SGILD ETL QMSQ+
Sbjct: 61  TYLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMSQS 120

Query: 121 RCSIPDIFENGDNETSVTTSNLHIGSKYTFFPGRVKWPSWKKYQLTYSFLRNFPEGFKES 180
           RCS+PD FE+ DNETS+TTSNLH+GS++ FFPGR KWP  K Y LT+SF+ NFP  FK  
Sbjct: 121 RCSVPDFFESDDNETSMTTSNLHMGSRFKFFPGRPKWPDSKNYSLTFSFINNFPGIFKGE 180

Query: 181 VRTAFMIWYERSRFNFTEVDENKGADIRISFEVGNHGDWYPFKKGSGVLAHTFGPGDGRF 240
           V  AF+ WYERSRF FTEV E + +DI+ISFEVG+HGD +PF+   GVLAH F P DGR 
Sbjct: 181 VGDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPFR--VGVLAHAFTPTDGRL 240

Query: 241 HFNADQPFSVEVTYGMYHLRTIALHELGHALGLAHSTNENAIMFPTVPPNVVRDLDMDDV 300
           HFN D+PFS EV  G YH+R++ALHELGH+LGLAH+   +AIM+PT+PPN  + ++ DDV
Sbjct: 241 HFNGDEPFSSEVAEGKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAKSINSDDV 300

Query: 301 NGLWDLYDGF 310
           NGLW LYD F
Sbjct: 301 NGLWALYDNF 308

[Close]
BLAST of Lsi02G015320 vs. TrEMBL
Match: A0A0A0KNY8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G522910 PE=3 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 5.4e-65
Identity = 144/310 (46.45%), Postives = 186/310 (60.00%), Query Frame = 1 

Query: 1   MDFKSSSLQLFLLFLASIAFHPISARDLDDIHKSSQFLFPQHLLGSRKGHNIEGIHSIKT 60
           M FKS  LQL  L L +IA H  S        K +  L PQ L G RKG N+EGIHSIK 
Sbjct: 1   MAFKS--LQLLFLVLVTIASHVSS--------KHNPILSPQRLYGCRKGDNVEGIHSIKK 60

Query: 61  HLQRYGYLNKNYNI----IDTNSAYNNAFDDHLESAVKKYQKFFKLNESGILDVETLHQM 120
           +LQRYGYL+ N +I    I+ NS   N FDD LESA+K YQK+  LN SGILD ETL QM
Sbjct: 61  YLQRYGYLSHNTSIDSHIIELNS---NKFDDSLESAIKLYQKWSHLNVSGILDQETLDQM 120

Query: 121 SQARCSIPDIFENGDNETSVTTSNLHIGSKYTFFPGRVKWPSWKKYQLTYSFLRNFPEGF 180
            + RC + D+F+   N+      +L + S YT FPG +KWP +K++ LTY F  NFP  F
Sbjct: 121 FKPRCGVRDVFKFNSNKN--LEDDLEMSSHYTLFPGNLKWPDYKRH-LTYVFTNNFPVDF 180

Query: 181 KESVRTAFMIWYERSRFNFTEVDENKGADIRISFEVGNHGDWYPFKKGSGVLAHTFGPGD 240
             SV  A   W  +S F F+E  + + ADI ISF++ +H D  PF    GV+ H F P D
Sbjct: 181 VPSVTEAMARWAAQSLFTFSEASDAQSADINISFQIKDHADGLPFDGPGGVVGHAFAPTD 240

Query: 241 GRFHFNADQPFSVEVTYGMYHLRTIALHELGHALGLAHSTNENAIMFPTVPPNVVRDLDM 300
           GR H + D  +S  +     ++  +ALHELGH LGLAHST   A+M+P +  N +++L+ 
Sbjct: 241 GRLHLDGDDTWSAGMEVQKVNVMNVALHELGHVLGLAHSTLPQAVMWPYIDSNALKNLND 294

Query: 301 DDVNGLWDLY 307
           DD+ GL  LY
Sbjct: 301 DDIAGLHALY 294

[Close]
BLAST of Lsi02G015320 vs. TrEMBL
Match: A0A0A0KVD8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G633150 PE=3 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 2.7e-64
Identity = 142/304 (46.71%), Postives = 183/304 (60.20%), Query Frame = 1 

Query: 7   SLQLFLLFLASIAFHPISARDLDDIHKSSQFLFPQHLLGSRKGHNIEGIHSIKTHLQRYG 66
           SLQL  L LASIA H IS         ++Q L P+ L G RKG N+EGIH+IK +LQRYG
Sbjct: 4   SLQLLFLVLASIASHVISI--------NNQILSPRRLNGCRKGDNVEGIHNIKKYLQRYG 63

Query: 67  YLNKNY----NIIDTNSAYNNAFDDHLESAVKKYQKFFKLNESGILDVETLHQMSQARCS 126
           YL+ N     NII+ NS   N FDD LESA++ YQK+  LN SGILD ETL QM Q RC 
Sbjct: 64  YLSHNTSTDSNIIELNS---NKFDDSLESAIRLYQKWSHLNVSGILDQETLDQMFQQRCG 123

Query: 127 IPDIFENGDNETSVTTSNLHIGSKYTFFPGRVKWPSWKKYQLTYSFLRNFPEGFKESVRT 186
           I D+F+   N +     +L + S Y  FP   KWP +K++ LTY F  NFP  F  SV  
Sbjct: 124 IRDVFK--FNSSKNLEDDLEMSSHYVLFPNNKKWPDYKRH-LTYMFTNNFPVDFVPSVTE 183

Query: 187 AFMIWYERSRFNFTEVDENKGADIRISFEVGNHGDWYPFKKGSGVLAHTFGPGDGRFHFN 246
           A   W  +S F F+E  + + ADI ISF+V +H D   F    GV+ H F P DGR H +
Sbjct: 184 AMARWAAQSLFTFSEASDAQSADINISFQVKDHADGSAFDGPGGVVGHAFAPTDGRLHLD 243

Query: 247 ADQPFSVEVTYGMYHLRTIALHELGHALGLAHSTNENAIMFPTVPPNVVRDLDMDDVNGL 306
            D  +S  +    +++  +ALHELGH LGLAHST   A+M+P +  N +++L+ DD+ GL
Sbjct: 244 GDDSWSAGLEENKFNVMNVALHELGHVLGLAHSTLPQAVMWPYIDSNALKNLNDDDIAGL 293

[Close]
BLAST of Lsi02G015320 vs. TrEMBL
Match: A0A0A0KU02_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632080 PE=3 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 5.9e-64
Identity = 152/304 (50.00%), Postives = 188/304 (61.84%), Query Frame = 1 

Query: 7   SLQLFLLFLASIAFHPISARDLDDIHKSSQFLFPQHLLGSRKGHNIEGIHSIKTHLQRYG 66
           SLQLFLL +ASIA H I+AR +   H SS      HL GSR G+NIEGIH++K +L+RYG
Sbjct: 6   SLQLFLL-IASIAPHVITAR-VRHTHISS------HLQGSRIGNNIEGIHNVKLYLKRYG 65

Query: 67  YLNKNYNIIDTNSAYNNAFDDHLESAVKKYQKFFKLNESGILDVETLHQMSQARCSIPDI 126
           YL    N+  TNS  NN FD  LESA+K +QK+  LN SG++D ETL  MS  RC IPDI
Sbjct: 66  YLT---NVESTNS--NNTFDVLLESAIKTFQKYHSLNVSGVIDEETLTLMSLPRCGIPDI 125

Query: 127 FEN---GDNETSVTTSNLHIGSKYTFFPGRVKWPSWKKYQLTYSFLRNFPEGFKESVRTA 186
             N    DN   + +S+ H  S +TFFP  +KWP   KY L Y+FL +FP  FKE V  A
Sbjct: 126 MHNINVMDNNVQMNSSSFH--SHFTFFPDNLKWPV-SKYNLRYTFLDDFPNDFKEPVMNA 185

Query: 187 FMIWYERSRFNFTEVDENKGADIRISFEVGNHGDWYPFKKGSGVLAHTFGPGDGRFHFNA 246
              W   S F F+E  E + ADI  +F  GNHGD YPF    GVLAH FGP DGR HF+ 
Sbjct: 186 MEQWALFSLFRFSEAVEAQEADITFNFVRGNHGDGYPFDGKGGVLAHAFGPLDGRVHFDW 245

Query: 247 DQPFSVEVTYGMYHLRTIALHELGHALGLAHSTNENAIMFPTVPP-NVVRDLDMDDVNGL 306
           D+ ++     G  ++  +ALHELGH LGLAHST  +AIM+P +      R L  DD+ G+
Sbjct: 246 DEDWADGSVGGFINVGMVALHELGHVLGLAHSTIRDAIMWPYMEAGEQTRGLQFDDIEGI 293

[Close]
BLAST of Lsi02G015320 vs. TAIR10
Match: AT1G70170.1 (AT1G70170.1 matrix metalloproteinase)

HSP 1 Score: 167.2 bits (422), Expect = 1.7e-41
Identity = 109/322 (33.85%), Postives = 165/322 (51.24%), Query Frame = 1 

Query: 8   LQLFLLFL-ASIAFHPISARDLDDIHKSSQFLFP--QHLLGSRKGHNIEGIHSIKTHLQR 67
           L LFL+   AS  F P S      +  +++  +    +  G   G N++G++ IK + QR
Sbjct: 9   LSLFLIVSPASAWFFPNSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKKYFQR 68

Query: 68  YGYLNKNYNIIDTNSAYNNAFDDHLESAVKKYQKFFKLNESGILDVETLHQMSQARCSIP 127
           +GY+ + ++       + + FDD L++AV+ YQ  F LN +G LD  T+  +   RC  P
Sbjct: 69  FGYIPETFS-----GNFTDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNP 128

Query: 128 DIFENGDN----------ETSVTTSNLHIGSKYTFFPGRVKWPSWKKYQLTYSFLRNFP- 187
           D+  NG +          E + + ++LH   +YT FPG  +WP  ++  LTY+F    P 
Sbjct: 129 DVV-NGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRR-DLTYAFDPKNPL 188

Query: 188 -EGFKESVRTAFMIWYERSRFNFTEVDENKGADIRISFEVGNHGDWYPFKKGSGVLAHTF 247
            E  K     AF  W + +  NFT  +    +DI I F  G+HGD  PF    G LAH F
Sbjct: 189 TEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAF 248

Query: 248 GPGDGRFHFNADQPFSVEVTYGMY-------HLRTIALHELGHALGLAHSTNENAIMFPT 307
            P  G+FH +AD+ + V      +        L ++A+HE+GH LGL HS+ E +IM+PT
Sbjct: 249 SPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPT 308

[Close]
BLAST of Lsi02G015320 vs. TAIR10
Match: AT1G59970.1 (AT1G59970.1 Matrixin family protein)

HSP 1 Score: 163.3 bits (412), Expect = 2.4e-40
Identity = 102/283 (36.04%), Postives = 142/283 (50.18%), Query Frame = 1 

Query: 43  LLGSRKGHNIEGIHSIKTHLQRYGYLNKNYNIIDTNSAYNNAFDDHLESAVKKYQKFFKL 102
           L G   G NI G+  +K + +R+GY+    N  D        FDD L+SA+  YQK F L
Sbjct: 48  LAGCHIGENINGLSKLKQYFRRFGYITTTGNCTDD-------FDDVLQSAINTYQKNFNL 107

Query: 103 NESGILDVETLHQMSQARCSIPDIFENGDNETSVTTSNLHIGSKYTFFPGRVKWPSWKKY 162
             +G LD  TL Q+ + RC  PD+ + G +E +     L    KY+FFPG+ +WP  +K 
Sbjct: 108 KVTGKLDSSTLRQIVKPRCGNPDLID-GVSEMN-GGKILRTTEKYSFFPGKPRWPK-RKR 167

Query: 163 QLTYSFL--RNFPEGFKESVRTAFMIWYERSRFNFTEVDENKGADIRISFEVGNHGDWYP 222
            LTY+F    N  +  K     AF  W E +  NFT  +    ADI I F  G HGD  P
Sbjct: 168 DLTYAFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEP 227

Query: 223 FKKGSGVLAHTFGPGDGRFHFNADQPFSVE---------VTYGMYHLRTIALHELGHALG 282
           F    G LAH   P  G  H + D+ + +              +  L ++A+HE+GH LG
Sbjct: 228 FDGAMGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLG 287

Query: 283 LAHSTNENAIMFPTVPPNVVR-DLDMDDVNGLWDLYDGFDDAD 314
           L HS+ E+AIMFP +     + +L  DD+ G+  LY G  + D
Sbjct: 288 LGHSSVEDAIMFPAISGGDRKVELAKDDIEGIQHLYGGNPNGD 320

[Close]
BLAST of Lsi02G015320 vs. TAIR10
Match: AT4G16640.1 (AT4G16640.1 Matrixin family protein)

HSP 1 Score: 157.9 bits (398), Expect = 1.0e-38
Identity = 96/272 (35.29%), Postives = 143/272 (52.57%), Query Frame = 1 

Query: 49  GHNIEGIHSIKTHLQRYGYLNKNYNIIDTNSAYNNAFDDHLESAVKKYQKFFKLNESGIL 108
           G ++ G+  +K +L R+GY+N      D +  +++ FD  LESA+  YQ+   L  +G L
Sbjct: 64  GSHVSGVSELKRYLHRFGYVN------DGSEIFSDVFDGPLESAISLYQENLGLPITGRL 123

Query: 109 DVETLHQMSQARCSIPDIFENGDNETSVTTSN--LHIGSKYTFFPGRVKWPSWKKYQLTY 168
           D  T+  MS  RC + D        T +T +N  LH  + YT+F G+   P W +  LTY
Sbjct: 124 DTSTVTLMSLPRCGVSD--------THMTINNDFLHTTAHYTYFNGK---PKWNRDTLTY 183

Query: 169 SFLRNFP------EGFKESVRTAFMIWYERSRFNFTEVDENKGADIRISFEVGNHGDWYP 228
           +  +         E  K   R AF  W      +F EVD+   AD++I F  G+HGD  P
Sbjct: 184 AISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLP 243

Query: 229 FKKGSGVLAHTFGPGDGRFHFNADQPFSVE-----VTYGMYHLRTIALHELGHALGLAHS 288
           F    G LAH F P +GR H +A + + V+      +     L ++A HE+GH LGL HS
Sbjct: 244 FDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHS 303

Query: 289 TNENAIMFPTVPPNVVR-DLDMDDVNGLWDLY 307
           + E+A+M+P++ P   + DL +DDV G+  LY
Sbjct: 304 SQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]
BLAST of Lsi02G015320 vs. TAIR10
Match: AT1G24140.1 (AT1G24140.1 Matrixin family protein)

HSP 1 Score: 153.3 bits (386), Expect = 2.5e-37
Identity = 111/340 (32.65%), Postives = 162/340 (47.65%), Query Frame = 1 

Query: 10  LFLLFLASIAFHPISARDLDDIHKSSQFLFPQHL--------------LGSRKGHNIEGI 69
           +F++FL   A  P+SA      + +S  + PQ L               G   G   +G+
Sbjct: 6   VFMVFLLFFAPSPVSA----GFYTNSSAIPPQLLRNATGNPWNSFLNFTGCHAGKKYDGL 65

Query: 70  HSIKTHLQRYGYLNKNYNIIDTNSAYNNAFDDHLESAVKKYQKFFKLNESGILDVETLHQ 129
           + +K + Q +GY+ +     + +  + + FDD L++AV+ YQ+ F+LN +G+LD  TL  
Sbjct: 66  YMLKQYFQHFGYITET----NLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVLDELTLKH 125

Query: 130 MSQARCSIPDIFENGDNETSVTTSNLHIGSK------------------YTFFPGRVKWP 189
           +   RC  PD+  NG       TS +H G K                  Y+FFPG  +WP
Sbjct: 126 VVIPRCGNPDVV-NG-------TSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWP 185

Query: 190 SWKKYQLTYSF-LRN-FPEGFKESVRTAFMIWYERSRFNFTEVDENKGADIRISFEVGNH 249
             ++  LTY+F  RN   E  K     AF  W E +   FT V+    +DI I F  G H
Sbjct: 186 RNRR-DLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEH 245

Query: 250 GDWYPFKKGSGVLAHTFGPGDGRFHFNADQPFSVEVTYG--------MYHLRTIALHELG 307
           GD  PF      LAH F P  G FH + ++ + V    G           L ++A+HE+G
Sbjct: 246 GDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIG 305

[Close]
BLAST of Lsi02G015320 vs. TAIR10
Match: AT2G45040.1 (AT2G45040.1 Matrixin family protein)

HSP 1 Score: 153.3 bits (386), Expect = 2.5e-37
Identity = 96/263 (36.50%), Postives = 136/263 (51.71%), Query Frame = 1 

Query: 55  IHSIKTHLQRYGYLNKNYNIIDTNSAYNNAFDDHLESAVKKYQKFFKLNESGILDVETLH 114
           I  IK HLQ+YGYL +N    D +           E A+ +YQK   L  +G  D +TL 
Sbjct: 50  IPEIKRHLQQYGYLPQNKESDDVS----------FEQALVRYQKNLGLPITGKPDSDTLS 109

Query: 115 QMSQARCSIPDIFENGDNETSVTTSNLHIGSKYTFFPGRVKWPSWKKYQLTYSFLRN--- 174
           Q+   RC  PD       +    T+  H G KY +FPGR +W      +LTY+F +    
Sbjct: 110 QILLPRCGFPD-------DVEPKTAPFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLT 169

Query: 175 ---FPEGFKESVRTAFMIWYERSRFNFTEVDENKGADIRISFEVGNHGDWYPFKKGSGVL 234
               P   +   R AF  W      +F E ++   ADI+I F  G+HGD  PF    GVL
Sbjct: 170 PYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVL 229

Query: 235 AHTFGPGDGRFHFNADQPFSV----EVTYGMYHLRTIALHELGHALGLAHSTNENAIMFP 294
           AHTF P +GR H +  + ++V    E +     L ++A+HE+GH LGL HS+ ++A M+P
Sbjct: 230 AHTFSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYP 289

Query: 295 TVPPNVVR-DLDMDDVNGLWDLY 307
           T+ P   + +L+MDDV G+  LY
Sbjct: 290 TLKPRSKKVNLNMDDVVGVQSLY 295

[Close]
BLAST of Lsi02G015320 vs. NCBI nr
Match: gi|449449423|ref|XP_004142464.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 496.9 bits (1278), Expect = 2.6e-137
Identity = 243/311 (78.14%), Postives = 269/311 (86.50%), Query Frame = 1 

Query: 1   MDFKSSS-LQLFLLFL-ASIAFHPISARDLDDIHKSSQFLFPQHLLGSRKGHNIEGIHSI 60
           MDFKSSS LQ+FLL L ASIAF  I ARDL DI K SQ LFPQHLLGS KGHNIEGIH+I
Sbjct: 1   MDFKSSSDLQIFLLLLLASIAFPFIWARDLHDI-KKSQLLFPQHLLGSSKGHNIEGIHTI 60

Query: 61  KTHLQRYGYLNKNYNIIDTNSAYNNAFDDHLESAVKKYQKFFKLNESGILDVETLHQMSQ 120
           K HLQRYGYL+KNYNIIDTN AYNNAFDDHLESA+KKYQ FFKL +SG+LD+ETLHQMSQ
Sbjct: 61  KMHLQRYGYLSKNYNIIDTNGAYNNAFDDHLESAIKKYQMFFKLPKSGVLDMETLHQMSQ 120

Query: 121 ARCSIPDIFENGDNETSVTTSNLHIGSKYTFFPGRVKWPSWKKYQLTYSFLRNFPEGFKE 180
           ARCS+PDIFEN +NETSVTTSNLHIGSKYTFFPGRVKWP    Y+LTY+ + NFPE FKE
Sbjct: 121 ARCSVPDIFENNENETSVTTSNLHIGSKYTFFPGRVKWPDSLNYRLTYALVNNFPEEFKE 180

Query: 181 SVRTAFMIWYERSRFNFTEVDENKGADIRISFEVGNHGDWYPFKKGSGVLAHTFGPGDGR 240
           SVRTAF IWY RSRFNFTEV EN+G +IRISFE G HGD++PF K S  LAHTF P DGR
Sbjct: 181 SVRTAFEIWYGRSRFNFTEVSENEGGNIRISFERGVHGDYHPFTKNSKTLAHTFAPIDGR 240

Query: 241 FHFNADQPFSVEVTYGMYHLRTIALHELGHALGLAHSTNENAIMFPTVPPNVVRDLDMDD 300
           FHFNAD+PFSV+VTY  YHLRT+ALHELGHA GLAHS +E+AIMFPT+P N+ +DLDMDD
Sbjct: 241 FHFNADKPFSVDVTYNAYHLRTVALHELGHAFGLAHSPSEDAIMFPTIPTNLEKDLDMDD 300

Query: 301 VNGLWDLYDGF 310
           + GLW+LYDGF
Sbjct: 301 IEGLWELYDGF 310

[Close]
BLAST of Lsi02G015320 vs. NCBI nr
Match: gi|700197234|gb|KGN52411.1| (hypothetical protein Csa_5G632100 [Cucumis sativus])

HSP 1 Score: 439.1 bits (1128), Expect = 6.4e-120
Identity = 207/310 (66.77%), Postives = 252/310 (81.29%), Query Frame = 1 

Query: 1   MDFKSSSLQLFLLFLASIA-FHPISARDLDDIHKSSQFLFPQHLLGSRKGHNIEGIHSIK 60
           MDFKS  LQLFLL L  +  FHPIS+RDL+ IH  SQFLFP++L+GSRKGHN+EG H+IK
Sbjct: 1   MDFKSLHLQLFLLLLTFLLLFHPISSRDLNHIHGPSQFLFPKYLIGSRKGHNLEGTHTIK 60

Query: 61  THLQRYGYLNKNYNIIDTNSAYNNAFDDHLESAVKKYQKFFKLNESGILDVETLHQMSQA 120
           T+LQRYGYL+K+YNIIDTN  YNNA+D+ LES++KKYQKFF+LN+SGILD ETL QMSQ+
Sbjct: 61  TYLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMSQS 120

Query: 121 RCSIPDIFENGDNETSVTTSNLHIGSKYTFFPGRVKWPSWKKYQLTYSFLRNFPEGFKES 180
           RCS+PD FE+ DNETS+TTSNLH+GS++ FFPGR KWP  K Y LT+SF+ NFP  FK  
Sbjct: 121 RCSVPDFFESDDNETSMTTSNLHMGSRFKFFPGRPKWPDSKNYSLTFSFINNFPGIFKGE 180

Query: 181 VRTAFMIWYERSRFNFTEVDENKGADIRISFEVGNHGDWYPFKKGSGVLAHTFGPGDGRF 240
           V  AF+ WYERSRF FTEV E + +DI+ISFEVG+HGD +PF+   GVLAH F P DGR 
Sbjct: 181 VGDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPFR--VGVLAHAFTPTDGRL 240

Query: 241 HFNADQPFSVEVTYGMYHLRTIALHELGHALGLAHSTNENAIMFPTVPPNVVRDLDMDDV 300
           HFN D+PFS EV  G YH+R++ALHELGH+LGLAH+   +AIM+PT+PPN  + ++ DDV
Sbjct: 241 HFNGDEPFSSEVAEGKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAKSINSDDV 300

Query: 301 NGLWDLYDGF 310
           NGLW LYD F
Sbjct: 301 NGLWALYDNF 308

[Close]
BLAST of Lsi02G015320 vs. NCBI nr
Match: gi|659118021|ref|XP_008458908.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 415.6 bits (1067), Expect = 7.6e-113
Identity = 202/312 (64.74%), Postives = 243/312 (77.88%), Query Frame = 1 

Query: 1   MDFKSSSLQLFLLFLASIAFHPISARDLDDIHKSSQFLFPQHLLGSRKGHNIEGIHSIKT 60
           MDFKS  LQLFLL  +   FHPIS+RDL+ IH  SQFLFP++LLGSRKGHNIEG H+IKT
Sbjct: 1   MDFKSFYLQLFLLLTSLSLFHPISSRDLNHIHGPSQFLFPKYLLGSRKGHNIEGTHAIKT 60

Query: 61  HLQRYGYLNKNYNIIDTNSAYNNAFDDHLESAVKKYQKFFKLNESGILDVETLHQMSQAR 120
           +LQ YGYL+KNYNIIDTN  YNNA+D+ LE +VKKYQKFFKLNESGILD ETL QMSQ R
Sbjct: 61  YLQHYGYLSKNYNIIDTNGVYNNAYDEQLECSVKKYQKFFKLNESGILDRETLSQMSQPR 120

Query: 121 CSIPDIFENGDNETSVTTSNLHIGSKYTFFPGRVKWPSWKKYQLTYSFLRNFPEGFKESV 180
           CS+PDIFE+ DNETS+TTSNLHI S+Y FFPGR KW   +   LTYS ++NFPE F+ ++
Sbjct: 121 CSVPDIFESEDNETSITTSNLHIRSRYAFFPGRPKWSDSR--NLTYSLIKNFPEKFEAAI 180

Query: 181 RTAFMIWYERSRFNFTEVDENKGADIRISFEVGNHGDWYPFKKGSGVLAHTFGPGDGRFH 240
             AFM+W+ RSRF FT V E + +DI ISFEVG+HGD +PFK   GVLAH F P DGR H
Sbjct: 181 VDAFMLWFLRSRFCFTYVSEGQKSDITISFEVGDHGDGHPFK--GGVLAHAFSPEDGRLH 240

Query: 241 FNADQPFSVEVTYGMYHLRTIALHELGHALGLAHSTNENAIMFPTVPPNVVRDLDMDDVN 300
           FN D+ FS ++  G YH+RT+A HELGH+LGLAH+  E AIM+PT+P N  + L  DDV+
Sbjct: 241 FNGDKSFSSDLEEGKYHIRTVAAHELGHSLGLAHTNIEAAIMYPTLPSNFSKILSQDDVD 300

Query: 301 GLWDLYDGFDDA 313
           GLW+ Y   +D+
Sbjct: 301 GLWNFYGQSEDS 308

[Close]
BLAST of Lsi02G015320 vs. NCBI nr
Match: gi|778708743|ref|XP_011656274.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 271.2 bits (692), Expect = 2.3e-69
Identity = 124/194 (63.92%), Postives = 152/194 (78.35%), Query Frame = 1 

Query: 116 MSQARCSIPDIFENGDNETSVTTSNLHIGSKYTFFPGRVKWPSWKKYQLTYSFLRNFPEG 175
           MSQ+RCS+PD FE+ DNETS+TTSNLH+GS++ FFPGR KWP  K Y LT+SF+ NFP  
Sbjct: 1   MSQSRCSVPDFFESDDNETSMTTSNLHMGSRFKFFPGRPKWPDSKNYSLTFSFINNFPGI 60

Query: 176 FKESVRTAFMIWYERSRFNFTEVDENKGADIRISFEVGNHGDWYPFKKGSGVLAHTFGPG 235
           FK  V  AF+ WYERSRF FTEV E + +DI+ISFEVG+HGD +PF+   GVLAH F P 
Sbjct: 61  FKGEVGDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPFR--VGVLAHAFTPT 120

Query: 236 DGRFHFNADQPFSVEVTYGMYHLRTIALHELGHALGLAHSTNENAIMFPTVPPNVVRDLD 295
           DGR HFN D+PFS EV  G YH+R++ALHELGH+LGLAH+   +AIM+PT+PPN  + ++
Sbjct: 121 DGRLHFNGDEPFSSEVAEGKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAKSIN 180

Query: 296 MDDVNGLWDLYDGF 310
            DDVNGLW LYD F
Sbjct: 181 SDDVNGLWALYDNF 192

[Close]
BLAST of Lsi02G015320 vs. NCBI nr
Match: gi|778708586|ref|XP_004142843.2| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 256.1 bits (653), Expect = 7.7e-65
Identity = 144/310 (46.45%), Postives = 186/310 (60.00%), Query Frame = 1 

Query: 1   MDFKSSSLQLFLLFLASIAFHPISARDLDDIHKSSQFLFPQHLLGSRKGHNIEGIHSIKT 60
           M FKS  LQL  L L +IA H  S        K +  L PQ L G RKG N+EGIHSIK 
Sbjct: 1   MAFKS--LQLLFLVLVTIASHVSS--------KHNPILSPQRLYGCRKGDNVEGIHSIKK 60

Query: 61  HLQRYGYLNKNYNI----IDTNSAYNNAFDDHLESAVKKYQKFFKLNESGILDVETLHQM 120
           +LQRYGYL+ N +I    I+ NS   N FDD LESA+K YQK+  LN SGILD ETL QM
Sbjct: 61  YLQRYGYLSHNTSIDSHIIELNS---NKFDDSLESAIKLYQKWSHLNVSGILDQETLDQM 120

Query: 121 SQARCSIPDIFENGDNETSVTTSNLHIGSKYTFFPGRVKWPSWKKYQLTYSFLRNFPEGF 180
            + RC + D+F+   N+      +L + S YT FPG +KWP +K++ LTY F  NFP  F
Sbjct: 121 FKPRCGVRDVFKFNSNKN--LEDDLEMSSHYTLFPGNLKWPDYKRH-LTYVFTNNFPVDF 180

Query: 181 KESVRTAFMIWYERSRFNFTEVDENKGADIRISFEVGNHGDWYPFKKGSGVLAHTFGPGD 240
             SV  A   W  +S F F+E  + + ADI ISF++ +H D  PF    GV+ H F P D
Sbjct: 181 VPSVTEAMARWAAQSLFTFSEASDAQSADINISFQIKDHADGLPFDGPGGVVGHAFAPTD 240

Query: 241 GRFHFNADQPFSVEVTYGMYHLRTIALHELGHALGLAHSTNENAIMFPTVPPNVVRDLDM 300
           GR H + D  +S  +     ++  +ALHELGH LGLAHST   A+M+P +  N +++L+ 
Sbjct: 241 GRLHLDGDDTWSAGMEVQKVNVMNVALHELGHVLGLAHSTLPQAVMWPYIDSNALKNLND 294

Query: 301 DDVNGLWDLY 307
           DD+ GL  LY
Sbjct: 301 DDIAGLHALY 294

The following BLAST results are available for this feature:
BLAST of Lsi02G015320 vs. Swiss-Prot
Match NameE-valueIdentityDescription
MEP1_SOYBN2.3e-4037.08Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2[more]
2MMP_ARATH2.9e-4033.85Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1[more]
1MMP_ARATH1.8e-3735.29Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1[more]
3MMP_ARATH4.4e-3632.65Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1[more]
4MMP_ARATH4.4e-3636.50Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana GN=4MMP PE=1 SV=1[more]
BLAST of Lsi02G015320 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KS15_CUCSA1.8e-13778.14Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632090 PE=3 SV=1[more]
A0A0A0KX75_CUCSA4.5e-12066.77Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632100 PE=3 SV=1[more]
A0A0A0KNY8_CUCSA5.4e-6546.45Uncharacterized protein OS=Cucumis sativus GN=Csa_5G522910 PE=3 SV=1[more]
A0A0A0KVD8_CUCSA2.7e-6446.71Uncharacterized protein OS=Cucumis sativus GN=Csa_5G633150 PE=3 SV=1[more]
A0A0A0KU02_CUCSA5.9e-6450.00Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632080 PE=3 SV=1[more]
BLAST of Lsi02G015320 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G70170.11.7e-4133.85 matrix metalloproteinase[more]
AT1G59970.12.4e-4036.04 Matrixin family protein[more]
AT4G16640.11.0e-3835.29 Matrixin family protein[more]
AT1G24140.12.5e-3732.65 Matrixin family protein[more]
AT2G45040.12.5e-3736.50 Matrixin family protein[more]
BLAST of Lsi02G015320 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449449423|ref|XP_004142464.1|2.6e-13778.14PREDICTED: metalloendoproteinase 1-like [Cucumis sativus][more]
gi|700197234|gb|KGN52411.1|6.4e-12066.77hypothetical protein Csa_5G632100 [Cucumis sativus][more]
gi|659118021|ref|XP_008458908.1|7.6e-11364.74PREDICTED: metalloendoproteinase 1-like [Cucumis melo][more]
gi|778708743|ref|XP_011656274.1|2.3e-6963.92PREDICTED: metalloendoproteinase 1-like [Cucumis sativus][more]
gi|778708586|ref|XP_004142843.2|7.7e-6546.45PREDICTED: metalloendoproteinase 1-like [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0008237metallopeptidase activity
GO:0008270zinc ion binding
GO:0004222metalloendopeptidase activity
Vocabulary: Cellular Component
TermDefinition
GO:0031012extracellular matrix
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: INTERPRO
TermDefinition
IPR024079MetalloPept_cat_dom_sf
IPR021190Pept_M10A
IPR006026Peptidase_Metallo
IPR002477Peptidoglycan-bd-like
IPR001818Pept_M10_metallopeptidase
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0031012 extracellular matrix
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0008237 metallopeptidase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G015320.1Lsi02G015320.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001818Peptidase M10, metallopeptidasePFAMPF00413Peptidase_M10coord: 159..306
score: 4.0
IPR002477Peptidoglycan binding-likeGENE3DG3DSA:1.10.101.10coord: 54..118
score: 7.4
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 53..116
score: 5.3
IPR002477Peptidoglycan binding-likeunknownSSF47090PGBD-likecoord: 47..126
score: 3.79
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 152..308
score: 2.0
IPR021190Peptidase M10APRINTSPR00138MATRIXINcoord: 294..307
score: 4.5E-30coord: 204..232
score: 4.5E-30coord: 116..129
score: 4.5E-30coord: 261..286
score: 4.5E-30coord: 180..195
score: 4.5
IPR024079Metallopeptidase, catalytic domainGENE3DG3DSA:3.40.390.10coord: 119..307
score: 2.3
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 40..306
score: 7.2E-86coord: 1..22
score: 7.2
NoneNo IPR availablePANTHERPTHR10201:SF152SUBFAMILY NOT NAMEDcoord: 40..306
score: 7.2E-86coord: 1..22
score: 7.2
NoneNo IPR availableunknownSSF55486Metalloproteases ("zincins"), catalytic domaincoord: 155..307
score: 3.18

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Lsi02G015320MELO3C021497.2Melon (DHL92) v3.6.1lsimedB171
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi02G015320Cucumber (Chinese Long) v3cuclsiB028
Lsi02G015320Cucumber (Chinese Long) v3cuclsiB371
Lsi02G015320Watermelon (97103) v2lsiwmbB161
Lsi02G015320Watermelon (97103) v2lsiwmbB208
Lsi02G015320Wax gourdlsiwgoB207
Lsi02G015320Wax gourdlsiwgoB253
Lsi02G015320Bottle gourd (USVL1VR-Ls)lsilsiB089
Lsi02G015320Cucumber (Gy14) v1cgylsiB118
Lsi02G015320Cucumber (Gy14) v1cgylsiB251
Lsi02G015320Cucurbita maxima (Rimu)cmalsiB272
Lsi02G015320Cucurbita maxima (Rimu)cmalsiB372
Lsi02G015320Cucurbita maxima (Rimu)cmalsiB651
Lsi02G015320Cucurbita moschata (Rifu)cmolsiB261
Lsi02G015320Cucurbita moschata (Rifu)cmolsiB640
Lsi02G015320Cucurbita pepo (Zucchini)cpelsiB141
Lsi02G015320Cucurbita pepo (Zucchini)cpelsiB336
Lsi02G015320Wild cucumber (PI 183967)cpilsiB028
Lsi02G015320Wild cucumber (PI 183967)cpilsiB352
Lsi02G015320Cucumber (Chinese Long) v2culsiB028
Lsi02G015320Cucumber (Chinese Long) v2culsiB348
Lsi02G015320Melon (DHL92) v3.5.1lsimeB162
Lsi02G015320Melon (DHL92) v3.5.1lsimeB182
Lsi02G015320Watermelon (Charleston Gray)lsiwcgB155
Lsi02G015320Watermelon (Charleston Gray)lsiwcgB176
Lsi02G015320Watermelon (97103) v1lsiwmB170
Lsi02G015320Cucumber (Gy14) v2cgyblsiB024
Lsi02G015320Cucumber (Gy14) v2cgyblsiB319
Lsi02G015320Melon (DHL92) v3.6.1lsimedB191
Lsi02G015320Silver-seed gourdcarlsiB232
Lsi02G015320Silver-seed gourdcarlsiB623