Lsi02G015310 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi02G015310
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Matrix metalloproteinase
Location	chr02 : 21191519 .. 21192460 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTATTTCAAATCATCATCACTCCTACTCTTCCTACTTCTTCTTGTCTCTATAACATGTCACCCTATTTCGGCACGTGTTCTAAATCACATCCATAAATCATCTCAATTTCTATTTCCCCAATATCTTCTAGGAAGTCGTAAAGGTCACAACATCGAAGGAATCCATAGTATCAAAACGTACCTCCAACATTATGGTTACTTAAACAAAAACTATAACACAATCGATACCAATGGTGCTTATAATAACGCCTTCGACGACGACCTAGAATCCTCCGTAAAAAAATACCAAAAATTCTTCAAGCTTAACGAGAGCGGAATTTTAGACGTGGAGACATTGCACCGAATGTCACAGCCTCGATGTTCGGTTCCAGACATATTCGAGAACGAGGAGAACGAGACGAGCGTGATGACGAATAATCTCCACTTAAGGAGTAAGTACACATTTTTCCCAGGGAAACCAAAATGGCCGAGTTCGAAGAAATACAGTCTAAAATACTCATTCATTCGGAATTTCCCAGAAAAGTTTAAGGTGGGAGTGAGGGAAGCTTTTGATGCATGGTATGAACAAAGCCGATTTGGTTTCTCAGAAGTGGATCAGAATGAAAAGGCGGATATAAAAGTAAGCTTTGAAGTGGGAAACCATGGAGATGGGTATCCTTTTCGTAAGGGATCAGGAGTTTTGGCACATGCGTTTGCGCCTGTAGATGGGAGACTTCACTTCAATGCTGATCAATCTTTTTCAATTCAAGTTACATATGGTATGTTTCATTTGAGGTCTGTTGCAATACATGAGCTTGGACATACGCTTGGGCTAGCGCACAGCACGAGTCAAGAGGCCATCATGTTTCCCAGTCTACCTCCTAATTTAAGTAAGAGTTTAGATATGGACGATGTTAATGGACTGTGGGATTTATATGATGGATTTCATCAAAATGTCTAA

mRNA sequence

ATGTATTTCAAATCATCATCACTCCTACTCTTCCTACTTCTTCTTGTCTCTATAACATGTCACCCTATTTCGGCACGTGTTCTAAATCACATCCATAAATCATCTCAATTTCTATTTCCCCAATATCTTCTAGGAAGTCGTAAAGGTCACAACATCGAAGGAATCCATAGTATCAAAACGTACCTCCAACATTATGGTTACTTAAACAAAAACTATAACACAATCGATACCAATGGTGCTTATAATAACGCCTTCGACGACGACCTAGAATCCTCCGTAAAAAAATACCAAAAATTCTTCAAGCTTAACGAGAGCGGAATTTTAGACGTGGAGACATTGCACCGAATGTCACAGCCTCGATGTTCGGTTCCAGACATATTCGAGAACGAGGAGAACGAGACGAGCGTGATGACGAATAATCTCCACTTAAGGAGTAAGTACACATTTTTCCCAGGGAAACCAAAATGGCCGAGTTCGAAGAAATACAGTCTAAAATACTCATTCATTCGGAATTTCCCAGAAAAGTTTAAGGTGGGAGTGAGGGAAGCTTTTGATGCATGGTATGAACAAAGCCGATTTGGTTTCTCAGAAGTGGATCAGAATGAAAAGGCGGATATAAAAGTAAGCTTTGAAGTGGGAAACCATGGAGATGGGTATCCTTTTCGTAAGGGATCAGGAGTTTTGGCACATGCGTTTGCGCCTGTAGATGGGAGACTTCACTTCAATGCTGATCAATCTTTTTCAATTCAAGTTACATATGGTATGTTTCATTTGAGGTCTGTTGCAATACATGAGCTTGGACATACGCTTGGGCTAGCGCACAGCACGAGTCAAGAGGCCATCATGTTTCCCAGTCTACCTCCTAATTTAAGTAAGAGTTTAGATATGGACGATGTTAATGGACTGTGGGATTTATATGATGGATTTCATCAAAATGTCTAA

Coding sequence (CDS)

ATGTATTTCAAATCATCATCACTCCTACTCTTCCTACTTCTTCTTGTCTCTATAACATGTCACCCTATTTCGGCACGTGTTCTAAATCACATCCATAAATCATCTCAATTTCTATTTCCCCAATATCTTCTAGGAAGTCGTAAAGGTCACAACATCGAAGGAATCCATAGTATCAAAACGTACCTCCAACATTATGGTTACTTAAACAAAAACTATAACACAATCGATACCAATGGTGCTTATAATAACGCCTTCGACGACGACCTAGAATCCTCCGTAAAAAAATACCAAAAATTCTTCAAGCTTAACGAGAGCGGAATTTTAGACGTGGAGACATTGCACCGAATGTCACAGCCTCGATGTTCGGTTCCAGACATATTCGAGAACGAGGAGAACGAGACGAGCGTGATGACGAATAATCTCCACTTAAGGAGTAAGTACACATTTTTCCCAGGGAAACCAAAATGGCCGAGTTCGAAGAAATACAGTCTAAAATACTCATTCATTCGGAATTTCCCAGAAAAGTTTAAGGTGGGAGTGAGGGAAGCTTTTGATGCATGGTATGAACAAAGCCGATTTGGTTTCTCAGAAGTGGATCAGAATGAAAAGGCGGATATAAAAGTAAGCTTTGAAGTGGGAAACCATGGAGATGGGTATCCTTTTCGTAAGGGATCAGGAGTTTTGGCACATGCGTTTGCGCCTGTAGATGGGAGACTTCACTTCAATGCTGATCAATCTTTTTCAATTCAAGTTACATATGGTATGTTTCATTTGAGGTCTGTTGCAATACATGAGCTTGGACATACGCTTGGGCTAGCGCACAGCACGAGTCAAGAGGCCATCATGTTTCCCAGTCTACCTCCTAATTTAAGTAAGAGTTTAGATATGGACGATGTTAATGGACTGTGGGATTTATATGATGGATTTCATCAAAATGTCTAA

Protein sequence

MYFKSSSLLLFLLLLVSITCHPISARVLNHIHKSSQFLFPQYLLGSRKGHNIEGIHSIKTYLQHYGYLNKNYNTIDTNGAYNNAFDDDLESSVKKYQKFFKLNESGILDVETLHRMSQPRCSVPDIFENEENETSVMTNNLHLRSKYTFFPGKPKWPSSKKYSLKYSFIRNFPEKFKVGVREAFDAWYEQSRFGFSEVDQNEKADIKVSFEVGNHGDGYPFRKGSGVLAHAFAPVDGRLHFNADQSFSIQVTYGMFHLRSVAIHELGHTLGLAHSTSQEAIMFPSLPPNLSKSLDMDDVNGLWDLYDGFHQNV

Homology

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BLAST of Lsi02G015310 vs. Swiss-Prot
Match: MEP1_SOYBN (Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2)

HSP 1 Score: 185.3 bits (469), Expect = 1.0e-45
Identity = 110/267 (41.20%), Postives = 143/267 (53.56%), Query Frame = 1


Query: 49  GHNIEGIHSIKTYLQHYGYLNKNYNTIDTNGAYNNAFDDDLESSVKKYQKFFKLNESGIL 108
           G N +G+ ++K Y  H GY+  N    D N      FDD L S++K YQK + LN +G  
Sbjct: 48  GQNYKGLSNVKNYFHHLGYI-PNAPHFDDN------FDDTLVSAIKTYQKNYNLNVTGKF 107

Query: 109 DVETLHRMSQPRCSVPDIFENEENETSVMTNNLHLRSKYTFFPGKPKWPSSKKYSLKYSF 168
           D+ TL ++  PRC VPDI  N    TS       + S YTFF   P+W +     L Y+F
Sbjct: 108 DINTLKQIMTPRCGVPDIIINTNKTTS-----FGMISDYTFFKDMPRWQAGTT-QLTYAF 167

Query: 169 IRN--FPEKFKVGVREAFDAWYEQSRFGFSEVDQNEKADIKVSFEVGNHGDGYPFRKGSG 228
                  + FK  +  AF  W       F E    E A+IK+ F   NHGD YPF    G
Sbjct: 168 SPEPRLDDTFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGG 227

Query: 229 VLAHAFAPVDGRLHFNADQSF--SIQVT----YGMFHLRSVAIHELGHTLGLAHSTSQEA 288
           +L HAFAP DGR HF+AD+ +  S  VT       F L SVA+HE+GH LGL HS+   A
Sbjct: 228 ILGHAFAPTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRA 287

Query: 289 IMFPSLPPNLSK-SLDMDDVNGLWDLY 307
           IM+PS+PP   K +L  DD++G+  LY
Sbjct: 288 IMYPSIPPRTRKVNLAQDDIDGIRKLY 301

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BLAST of Lsi02G015310 vs. Swiss-Prot
Match: 5MMP_ARATH (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 6.3e-43
Identity = 115/324 (35.49%), Postives = 161/324 (49.69%), Query Frame = 1


Query: 9   LLFLLLLVSITCHPISARVLNHIHKSSQFLFPQY----------LLGSRKGHNIEGIHSI 68
           LL  +L+   T +PISA+   ++       F             L G   G NI G+  +
Sbjct: 4   LLLTILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKL 63

Query: 69  KTYLQHYGYLNKNYNTIDTNGAYNNAFDDDLESSVKKYQKFFKLNESGILDVETLHRMSQ 128
           K Y + +GY       I T G   + FDD L+S++  YQK F L  +G LD  TL ++ +
Sbjct: 64  KQYFRRFGY-------ITTTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVK 123

Query: 129 PRCSVPDIFENEENETSVMTNNLHLRS--KYTFFPGKPKWPSSKKYSLKYSFI--RNFPE 188
           PRC  PD+ +      S M     LR+  KY+FFPGKP+WP  K+  L Y+F    N  +
Sbjct: 124 PRCGNPDLIDG----VSEMNGGKILRTTEKYSFFPGKPRWPKRKR-DLTYAFAPQNNLTD 183

Query: 189 KFKVGVREAFDAWYEQSRFGFSEVDQNEKADIKVSFEVGNHGDGYPFRKGSGVLAHAFAP 248
           + K     AF  W E +   F+  +   +ADI + F  G HGDG PF    G LAHA +P
Sbjct: 184 EVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASSP 243

Query: 249 VDGRLHFNADQSFSIQ---------VTYGMFHLRSVAIHELGHTLGLAHSTSQEAIMFPS 308
             G LH + D+ + I              +  L SVA+HE+GH LGL HS+ ++AIMFP+
Sbjct: 244 PTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFPA 303

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BLAST of Lsi02G015310 vs. Swiss-Prot
Match: 2MMP_ARATH (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 5.4e-42
Identity = 98/281 (34.88%), Postives = 151/281 (53.74%), Query Frame = 1


Query: 45  GSRKGHNIEGIHSIKTYLQHYGYLNKNYNTIDTNGAYNNAFDDDLESSVKKYQKFFKLNE 104
           G   G N++G++ IK Y Q +GY+ + ++     G + + FDD L+++V+ YQ  F LN 
Sbjct: 49  GCHHGQNVDGLYRIKKYFQRFGYIPETFS-----GNFTDDFDDILKAAVELYQTNFNLNV 108

Query: 105 SGILDVETLHRMSQPRCSVPDIFEN---------EENETSVMTNNLHLRSKYTFFPGKPK 164
           +G LD  T+  +  PRC  PD+            +  E +    +LH   +YT FPG+P+
Sbjct: 109 TGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPR 168

Query: 165 WPSSKKYSLKYSFIRNFP--EKFKVGVREAFDAWYEQSRFGFSEVDQNEKADIKVSFEVG 224
           WP +++  L Y+F    P  E+ K     AF  W + +   F+  +    +DI + F  G
Sbjct: 169 WPRNRR-DLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTG 228

Query: 225 NHGDGYPFRKGSGVLAHAFAPVDGRLHFNADQSFSIQVTYGMF-------HLRSVAIHEL 284
           +HGDG PF    G LAHAF+P  G+ H +AD+++ +      F        L SVA+HE+
Sbjct: 229 DHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEI 288

Query: 285 GHTLGLAHSTSQEAIMFPSLPPNLSK-SLDMDDVNGLWDLY 307
           GH LGL HS+ +E+IM+P++     K  L  DDV G+  LY
Sbjct: 289 GHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

[Close]

BLAST of Lsi02G015310 vs. Swiss-Prot
Match: 1MMP_ARATH (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 2.7e-41
Identity = 110/292 (37.67%), Postives = 151/292 (51.71%), Query Frame = 1


Query: 30  HIHKSSQFLFPQYLLGSRKGHNIEGIHSIKTYLQHYGYLNKNYNTIDTNGAYNNAFDDDL 89
           H+  S+   F + L+  + G ++ G+  +K YL  +GY+N      D +  +++ FD  L
Sbjct: 46  HVSNSTWHDFSR-LVDVQIGSHVSGVSELKRYLHRFGYVN------DGSEIFSDVFDGPL 105

Query: 90  ESSVKKYQKFFKLNESGILDVETLHRMSQPRCSVPDIFENEENETSVMTNN--LHLRSKY 149
           ES++  YQ+   L  +G LD  T+  MS PRC V D        T +  NN  LH  + Y
Sbjct: 106 ESAISLYQENLGLPITGRLDTSTVTLMSLPRCGVSD--------THMTINNDFLHTTAHY 165

Query: 150 TFFPGKPKWPS-------SKKYSLKYSFIRNFPEKFKVGVREAFDAWYEQSRFGFSEVDQ 209
           T+F GKPKW         SK + L Y       E  K   R AF  W       F EVD 
Sbjct: 166 TYFNGKPKWNRDTLTYAISKTHKLDYLT----SEDVKTVFRRAFSQWSSVIPVSFEEVDD 225

Query: 210 NEKADIKVSFEVGNHGDGYPFRKGSGVLAHAFAPVDGRLHFNADQSFSIQ-----VTYGM 269
              AD+K+ F  G+HGDG PF    G LAHAFAP +GRLH +A +++ +       +   
Sbjct: 226 FTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVA 285

Query: 270 FHLRSVAIHELGHTLGLAHSTSQEAIMFPSLPPNLSK-SLDMDDVNGLWDLY 307
             L SVA HE+GH LGL HS+ + A+M+PSL P   K  L +DDV G+  LY
Sbjct: 286 VDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]

BLAST of Lsi02G015310 vs. Swiss-Prot
Match: 3MMP_ARATH (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 3.6e-38
Identity = 108/338 (31.95%), Postives = 169/338 (50.00%), Query Frame = 1


Query: 8   LLLFLLLLVSITCHPISARVLNHIHKSSQFLFPQYL--------------LGSRKGHNIE 67
           + +F++ L+     P+SA      + +S  + PQ L               G   G   +
Sbjct: 4   ICVFMVFLLFFAPSPVSAG----FYTNSSAIPPQLLRNATGNPWNSFLNFTGCHAGKKYD 63

Query: 68  GIHSIKTYLQHYGYLNKNYNTIDTNGAYNNAFDDDLESSVKKYQKFFKLNESGILDVETL 127
           G++ +K Y QH+GY+ +     + +G + + FDD L+++V+ YQ+ F+LN +G+LD  TL
Sbjct: 64  GLYMLKQYFQHFGYITET----NLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVLDELTL 123

Query: 128 HRMSQPRCSVPDIFENEENETSVMTNN--------------LHLRSKYTFFPGKPKWPSS 187
             +  PRC  PD+     N TS M +                H    Y+FFPG+P+WP +
Sbjct: 124 KHVVIPRCGNPDVV----NGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPRN 183

Query: 188 KKYSLKYSFI-RN-FPEKFKVGVREAFDAWYEQSRFGFSEVDQNEKADIKVSFEVGNHGD 247
           ++  L Y+F  RN   E+ K     AF  W E +   F+ V++   +DI + F  G HGD
Sbjct: 184 RR-DLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGD 243

Query: 248 GYPFRKGSGVLAHAFAPVDGRLHFNADQSFSIQVTYG--------MFHLRSVAIHELGHT 307
           G PF      LAHAF+P  G  H + ++++ +    G           L SVA+HE+GH 
Sbjct: 244 GEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHL 303

[Close]

BLAST of Lsi02G015310 vs. TrEMBL
Match: A0A0A0KX75_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632100 PE=3 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 1.3e-119
Identity = 210/311 (67.52%), Postives = 256/311 (82.32%), Query Frame = 1


Query: 1   MYFKSSSLLLFLLLLVSITC-HPISARVLNHIHKSSQFLFPQYLLGSRKGHNIEGIHSIK 60
           M FKS  L LFLLLL  +   HPIS+R LNHIH  SQFLFP+YL+GSRKGHN+EG H+IK
Sbjct: 1   MDFKSLHLQLFLLLLTFLLLFHPISSRDLNHIHGPSQFLFPKYLIGSRKGHNLEGTHTIK 60

Query: 61  TYLQHYGYLNKNYNTIDTNGAYNNAFDDDLESSVKKYQKFFKLNESGILDVETLHRMSQP 120
           TYLQ YGYL+K+YN IDTNG YNNA+D+ LESS+KKYQKFF+LN+SGILD ETL +MSQ 
Sbjct: 61  TYLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMSQS 120

Query: 121 RCSVPDIFENEENETSVMTNNLHLRSKYTFFPGKPKWPSSKKYSLKYSFIRNFPEKFKVG 180
           RCSVPD FE+++NETS+ T+NLH+ S++ FFPG+PKWP SK YSL +SFI NFP  FK  
Sbjct: 121 RCSVPDFFESDDNETSMTTSNLHMGSRFKFFPGRPKWPDSKNYSLTFSFINNFPGIFKGE 180

Query: 181 VREAFDAWYEQSRFGFSEVDQNEKADIKVSFEVGNHGDGYPFRKGSGVLAHAFAPVDGRL 240
           V +AF AWYE+SRF F+EV + E++DIK+SFEVG+HGDG+PFR   GVLAHAF P DGRL
Sbjct: 181 VGDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPFR--VGVLAHAFTPTDGRL 240

Query: 241 HFNADQSFSIQVTYGMFHLRSVAIHELGHTLGLAHSTSQEAIMFPSLPPNLSKSLDMDDV 300
           HFN D+ FS +V  G +H+RSVA+HELGH+LGLAH+   +AIM+P+LPPN +KS++ DDV
Sbjct: 241 HFNGDEPFSSEVAEGKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAKSINSDDV 300

Query: 301 NGLWDLYDGFH 311
           NGLW LYD FH
Sbjct: 301 NGLWALYDNFH 309

[Close]

BLAST of Lsi02G015310 vs. TrEMBL
Match: A0A0A0KS15_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632090 PE=3 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 5.1e-116
Identity = 209/311 (67.20%), Postives = 249/311 (80.06%), Query Frame = 1


Query: 1   MYFKSSS-LLLFLLLLVSITCHP-ISARVLNHIHKSSQFLFPQYLLGSRKGHNIEGIHSI 60
           M FKSSS L +FLLLL++    P I AR L+ I K SQ LFPQ+LLGS KGHNIEGIH+I
Sbjct: 1   MDFKSSSDLQIFLLLLLASIAFPFIWARDLHDI-KKSQLLFPQHLLGSSKGHNIEGIHTI 60

Query: 61  KTYLQHYGYLNKNYNTIDTNGAYNNAFDDDLESSVKKYQKFFKLNESGILDVETLHRMSQ 120
           K +LQ YGYL+KNYN IDTNGAYNNAFDD LES++KKYQ FFKL +SG+LD+ETLH+MSQ
Sbjct: 61  KMHLQRYGYLSKNYNIIDTNGAYNNAFDDHLESAIKKYQMFFKLPKSGVLDMETLHQMSQ 120

Query: 121 PRCSVPDIFENEENETSVMTNNLHLRSKYTFFPGKPKWPSSKKYSLKYSFIRNFPEKFKV 180
            RCSVPDIFEN ENETSV T+NLH+ SKYTFFPG+ KWP S  Y L Y+ + NFPE+FK 
Sbjct: 121 ARCSVPDIFENNENETSVTTSNLHIGSKYTFFPGRVKWPDSLNYRLTYALVNNFPEEFKE 180

Query: 181 GVREAFDAWYEQSRFGFSEVDQNEKADIKVSFEVGNHGDGYPFRKGSGVLAHAFAPVDGR 240
            VR AF+ WY +SRF F+EV +NE  +I++SFE G HGD +PF K S  LAH FAP+DGR
Sbjct: 181 SVRTAFEIWYGRSRFNFTEVSENEGGNIRISFERGVHGDYHPFTKNSKTLAHTFAPIDGR 240

Query: 241 LHFNADQSFSIQVTYGMFHLRSVAIHELGHTLGLAHSTSQEAIMFPSLPPNLSKSLDMDD 300
            HFNAD+ FS+ VTY  +HLR+VA+HELGH  GLAHS S++AIMFP++P NL K LDMDD
Sbjct: 241 FHFNADKPFSVDVTYNAYHLRTVALHELGHAFGLAHSPSEDAIMFPTIPTNLEKDLDMDD 300

Query: 301 VNGLWDLYDGF 310
           + GLW+LYDGF
Sbjct: 301 IEGLWELYDGF 310

[Close]

BLAST of Lsi02G015310 vs. TrEMBL
Match: A0A0A0KNY8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G522910 PE=3 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 1.6e-64
Identity = 146/308 (47.40%), Postives = 190/308 (61.69%), Query Frame = 1


Query: 1   MYFKSSSLLLFLLLLVSITCHPISARVLNHIHKSSQFLFPQYLLGSRKGHNIEGIHSIKT 60
           M FKS  LL   L+LV+I  H  S        K +  L PQ L G RKG N+EGIHSIK 
Sbjct: 1   MAFKSLQLLF--LVLVTIASHVSS--------KHNPILSPQRLYGCRKGDNVEGIHSIKK 60

Query: 61  YLQHYGYLNKNYNTIDTN--GAYNNAFDDDLESSVKKYQKFFKLNESGILDVETLHRMSQ 120
           YLQ YGYL+ N  +ID++     +N FDD LES++K YQK+  LN SGILD ETL +M +
Sbjct: 61  YLQRYGYLSHN-TSIDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFK 120

Query: 121 PRCSVPDIFENEENETSVMTNNLHLRSKYTFFPGKPKWPSSKKYSLKYSFIRNFPEKFKV 180
           PRC V D+F+   N+   + ++L + S YT FPG  KWP  K++ L Y F  NFP  F  
Sbjct: 121 PRCGVRDVFKFNSNKN--LEDDLEMSSHYTLFPGNLKWPDYKRH-LTYVFTNNFPVDFVP 180

Query: 181 GVREAFDAWYEQSRFGFSEVDQNEKADIKVSFEVGNHGDGYPFRKGSGVLAHAFAPVDGR 240
            V EA   W  QS F FSE    + ADI +SF++ +H DG PF    GV+ HAFAP DGR
Sbjct: 181 SVTEAMARWAAQSLFTFSEASDAQSADINISFQIKDHADGLPFDGPGGVVGHAFAPTDGR 240

Query: 241 LHFNADQSFSIQVTYGMFHLRSVAIHELGHTLGLAHSTSQEAIMFPSLPPNLSKSLDMDD 300
           LH + D ++S  +     ++ +VA+HELGH LGLAHST  +A+M+P +  N  K+L+ DD
Sbjct: 241 LHLDGDDTWSAGMEVQKVNVMNVALHELGHVLGLAHSTLPQAVMWPYIDSNALKNLNDDD 294

Query: 301 VNGLWDLY 307
           + GL  LY
Sbjct: 301 IAGLHALY 294

[Close]

BLAST of Lsi02G015310 vs. TrEMBL
Match: A0A0A0KS35_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632070 PE=3 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 5.9e-64
Identity = 145/310 (46.77%), Postives = 193/310 (62.26%), Query Frame = 1


Query: 9   LLFLLLLVSITCHPISARVLNHIHKSSQFLFPQYLLGSRKGHNI-EGIHSIKTYLQHYGY 68
           L   LLL  +T H  SAR L H HKSS+ LFP++L+GS K  +I EGIH +K YLQ YGY
Sbjct: 7   LQLFLLLAFVTPHLTSARPL-HNHKSSRLLFPEHLIGSSKNDSIVEGIHKVKAYLQRYGY 66

Query: 69  L-NKNYNTIDTNGAYNNAFDDDLESSVKKYQKFFKLNESGILDVETLHRMSQPRCSVPDI 128
             N+N N + T+     AFDDDLES++K YQKF  L  SG+LD ETL +MS+PRC V D 
Sbjct: 67  FSNENENNLSTD-----AFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVADN 126

Query: 129 FEN------EENETSVMTNNLHLRSKYTFFPGKPKWPSSKKYSLKYSFIRNFPEKFKVGV 188
           F++      E N T+V     H    + FFPGK KWP  +K+ L Y F+ N+P K    V
Sbjct: 127 FQSVAQQDGENNNTTVQIGGSH----FMFFPGKGKWPY-RKWHLTYGFVHNYPMKHAAAV 186

Query: 189 REAFDAWYEQSRFGFSEVDQNEKADIKVSFEVGNHGDGYPFRKGSGVLAHAFAPVDGRLH 248
             AFD W   S+F FS   + + ADI +SFE G+HGDG PF    G+L HAF P+DGR+H
Sbjct: 187 VRAFDKWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAFGPIDGRVH 246

Query: 249 FNADQSFSI--QVTYGMFHLRSVAIHELGHTLGLAHSTSQEAIMFPSLPPNLSKS-LDMD 308
           F+AD+ ++    +T   F   SVA+HE+GH LGL HS    A+M+ ++   ++K+ L +D
Sbjct: 247 FDADEQWAEIGSLTNENFDFESVALHEIGHALGLGHSIFPSAVMWANMETGVNKTELTID 305

[Close]

BLAST of Lsi02G015310 vs. TrEMBL
Match: A0A0A0KU02_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632080 PE=3 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 8.6e-63
Identity = 145/302 (48.01%), Postives = 190/302 (62.91%), Query Frame = 1


Query: 7   SLLLFLLLLVSITCHPISARVLNHIHKSSQFLFPQYLLGSRKGHNIEGIHSIKTYLQHYG 66
           SL LFLL+  SI  H I+ARV  H H SS      +L GSR G+NIEGIH++K YL+ YG
Sbjct: 6   SLQLFLLI-ASIAPHVITARV-RHTHISS------HLQGSRIGNNIEGIHNVKLYLKRYG 65

Query: 67  YLNKNYNTIDTNGAYNNAFDDDLESSVKKYQKFFKLNESGILDVETLHRMSQPRCSVPDI 126
           YL    N   TN   NN FD  LES++K +QK+  LN SG++D ETL  MS PRC +PDI
Sbjct: 66  YLT---NVESTNS--NNTFDVLLESAIKTFQKYHSLNVSGVIDEETLTLMSLPRCGIPDI 125

Query: 127 FEN-EENETSVMTNNLHLRSKYTFFPGKPKWPSSKKYSLKYSFIRNFPEKFKVGVREAFD 186
             N    + +V  N+    S +TFFP   KWP SK Y+L+Y+F+ +FP  FK  V  A +
Sbjct: 126 MHNINVMDNNVQMNSSSFHSHFTFFPDNLKWPVSK-YNLRYTFLDDFPNDFKEPVMNAME 185

Query: 187 AWYEQSRFGFSEVDQNEKADIKVSFEVGNHGDGYPFRKGSGVLAHAFAPVDGRLHFNADQ 246
            W   S F FSE  + ++ADI  +F  GNHGDGYPF    GVLAHAF P+DGR+HF+ D+
Sbjct: 186 QWALFSLFRFSEAVEAQEADITFNFVRGNHGDGYPFDGKGGVLAHAFGPLDGRVHFDWDE 245

Query: 247 SFSIQVTYGMFHLRSVAIHELGHTLGLAHSTSQEAIMFPSLPP-NLSKSLDMDDVNGLWD 306
            ++     G  ++  VA+HELGH LGLAHST ++AIM+P +     ++ L  DD+ G+  
Sbjct: 246 DWADGSVGGFINVGMVALHELGHVLGLAHSTIRDAIMWPYMEAGEQTRGLQFDDIEGIQT 293

[Close]

BLAST of Lsi02G015310 vs. TAIR10
Match: AT1G59970.1 (AT1G59970.1 Matrixin family protein)

HSP 1 Score: 176.0 bits (445), Expect = 3.6e-44
Identity = 115/324 (35.49%), Postives = 161/324 (49.69%), Query Frame = 1


Query: 9   LLFLLLLVSITCHPISARVLNHIHKSSQFLFPQY----------LLGSRKGHNIEGIHSI 68
           LL  +L+   T +PISA+   ++       F             L G   G NI G+  +
Sbjct: 4   LLLTILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKL 63

Query: 69  KTYLQHYGYLNKNYNTIDTNGAYNNAFDDDLESSVKKYQKFFKLNESGILDVETLHRMSQ 128
           K Y + +GY       I T G   + FDD L+S++  YQK F L  +G LD  TL ++ +
Sbjct: 64  KQYFRRFGY-------ITTTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVK 123

Query: 129 PRCSVPDIFENEENETSVMTNNLHLRS--KYTFFPGKPKWPSSKKYSLKYSFI--RNFPE 188
           PRC  PD+ +      S M     LR+  KY+FFPGKP+WP  K+  L Y+F    N  +
Sbjct: 124 PRCGNPDLIDG----VSEMNGGKILRTTEKYSFFPGKPRWPKRKR-DLTYAFAPQNNLTD 183

Query: 189 KFKVGVREAFDAWYEQSRFGFSEVDQNEKADIKVSFEVGNHGDGYPFRKGSGVLAHAFAP 248
           + K     AF  W E +   F+  +   +ADI + F  G HGDG PF    G LAHA +P
Sbjct: 184 EVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASSP 243

Query: 249 VDGRLHFNADQSFSIQ---------VTYGMFHLRSVAIHELGHTLGLAHSTSQEAIMFPS 308
             G LH + D+ + I              +  L SVA+HE+GH LGL HS+ ++AIMFP+
Sbjct: 244 PTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFPA 303

[Close]

BLAST of Lsi02G015310 vs. TAIR10
Match: AT1G70170.1 (AT1G70170.1 matrix metalloproteinase)

HSP 1 Score: 172.9 bits (437), Expect = 3.0e-43
Identity = 98/281 (34.88%), Postives = 151/281 (53.74%), Query Frame = 1


Query: 45  GSRKGHNIEGIHSIKTYLQHYGYLNKNYNTIDTNGAYNNAFDDDLESSVKKYQKFFKLNE 104
           G   G N++G++ IK Y Q +GY+ + ++     G + + FDD L+++V+ YQ  F LN 
Sbjct: 49  GCHHGQNVDGLYRIKKYFQRFGYIPETFS-----GNFTDDFDDILKAAVELYQTNFNLNV 108

Query: 105 SGILDVETLHRMSQPRCSVPDIFEN---------EENETSVMTNNLHLRSKYTFFPGKPK 164
           +G LD  T+  +  PRC  PD+            +  E +    +LH   +YT FPG+P+
Sbjct: 109 TGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPR 168

Query: 165 WPSSKKYSLKYSFIRNFP--EKFKVGVREAFDAWYEQSRFGFSEVDQNEKADIKVSFEVG 224
           WP +++  L Y+F    P  E+ K     AF  W + +   F+  +    +DI + F  G
Sbjct: 169 WPRNRR-DLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTG 228

Query: 225 NHGDGYPFRKGSGVLAHAFAPVDGRLHFNADQSFSIQVTYGMF-------HLRSVAIHEL 284
           +HGDG PF    G LAHAF+P  G+ H +AD+++ +      F        L SVA+HE+
Sbjct: 229 DHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEI 288

Query: 285 GHTLGLAHSTSQEAIMFPSLPPNLSK-SLDMDDVNGLWDLY 307
           GH LGL HS+ +E+IM+P++     K  L  DDV G+  LY
Sbjct: 289 GHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

[Close]

BLAST of Lsi02G015310 vs. TAIR10
Match: AT4G16640.1 (AT4G16640.1 Matrixin family protein)

HSP 1 Score: 170.6 bits (431), Expect = 1.5e-42
Identity = 110/292 (37.67%), Postives = 151/292 (51.71%), Query Frame = 1


Query: 30  HIHKSSQFLFPQYLLGSRKGHNIEGIHSIKTYLQHYGYLNKNYNTIDTNGAYNNAFDDDL 89
           H+  S+   F + L+  + G ++ G+  +K YL  +GY+N      D +  +++ FD  L
Sbjct: 46  HVSNSTWHDFSR-LVDVQIGSHVSGVSELKRYLHRFGYVN------DGSEIFSDVFDGPL 105

Query: 90  ESSVKKYQKFFKLNESGILDVETLHRMSQPRCSVPDIFENEENETSVMTNN--LHLRSKY 149
           ES++  YQ+   L  +G LD  T+  MS PRC V D        T +  NN  LH  + Y
Sbjct: 106 ESAISLYQENLGLPITGRLDTSTVTLMSLPRCGVSD--------THMTINNDFLHTTAHY 165

Query: 150 TFFPGKPKWPS-------SKKYSLKYSFIRNFPEKFKVGVREAFDAWYEQSRFGFSEVDQ 209
           T+F GKPKW         SK + L Y       E  K   R AF  W       F EVD 
Sbjct: 166 TYFNGKPKWNRDTLTYAISKTHKLDYLT----SEDVKTVFRRAFSQWSSVIPVSFEEVDD 225

Query: 210 NEKADIKVSFEVGNHGDGYPFRKGSGVLAHAFAPVDGRLHFNADQSFSIQ-----VTYGM 269
              AD+K+ F  G+HGDG PF    G LAHAFAP +GRLH +A +++ +       +   
Sbjct: 226 FTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVA 285

Query: 270 FHLRSVAIHELGHTLGLAHSTSQEAIMFPSLPPNLSK-SLDMDDVNGLWDLY 307
             L SVA HE+GH LGL HS+ + A+M+PSL P   K  L +DDV G+  LY
Sbjct: 286 VDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]

BLAST of Lsi02G015310 vs. TAIR10
Match: AT1G24140.1 (AT1G24140.1 Matrixin family protein)

HSP 1 Score: 160.2 bits (404), Expect = 2.0e-39
Identity = 108/338 (31.95%), Postives = 169/338 (50.00%), Query Frame = 1


Query: 8   LLLFLLLLVSITCHPISARVLNHIHKSSQFLFPQYL--------------LGSRKGHNIE 67
           + +F++ L+     P+SA      + +S  + PQ L               G   G   +
Sbjct: 4   ICVFMVFLLFFAPSPVSAG----FYTNSSAIPPQLLRNATGNPWNSFLNFTGCHAGKKYD 63

Query: 68  GIHSIKTYLQHYGYLNKNYNTIDTNGAYNNAFDDDLESSVKKYQKFFKLNESGILDVETL 127
           G++ +K Y QH+GY+ +     + +G + + FDD L+++V+ YQ+ F+LN +G+LD  TL
Sbjct: 64  GLYMLKQYFQHFGYITET----NLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVLDELTL 123

Query: 128 HRMSQPRCSVPDIFENEENETSVMTNN--------------LHLRSKYTFFPGKPKWPSS 187
             +  PRC  PD+     N TS M +                H    Y+FFPG+P+WP +
Sbjct: 124 KHVVIPRCGNPDVV----NGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPRN 183

Query: 188 KKYSLKYSFI-RN-FPEKFKVGVREAFDAWYEQSRFGFSEVDQNEKADIKVSFEVGNHGD 247
           ++  L Y+F  RN   E+ K     AF  W E +   F+ V++   +DI + F  G HGD
Sbjct: 184 RR-DLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGD 243

Query: 248 GYPFRKGSGVLAHAFAPVDGRLHFNADQSFSIQVTYG--------MFHLRSVAIHELGHT 307
           G PF      LAHAF+P  G  H + ++++ +    G           L SVA+HE+GH 
Sbjct: 244 GEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHL 303

[Close]

BLAST of Lsi02G015310 vs. TAIR10
Match: AT2G45040.1 (AT2G45040.1 Matrixin family protein)

HSP 1 Score: 152.5 bits (384), Expect = 4.2e-37
Identity = 95/263 (36.12%), Postives = 136/263 (51.71%), Query Frame = 1


Query: 55  IHSIKTYLQHYGYLNKNYNTIDTNGAYNNAFDDDLESSVKKYQKFFKLNESGILDVETLH 114
           I  IK +LQ YGYL +N  + D +           E ++ +YQK   L  +G  D +TL 
Sbjct: 50  IPEIKRHLQQYGYLPQNKESDDVS----------FEQALVRYQKNLGLPITGKPDSDTLS 109

Query: 115 RMSQPRCSVPDIFENEENETSVMTNNLHLRSKYTFFPGKPKWPSSKKYSLKYSFIRN--- 174
           ++  PRC  PD  E +       T   H   KY +FPG+P+W       L Y+F +    
Sbjct: 110 QILLPRCGFPDDVEPK-------TAPFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLT 169

Query: 175 ---FPEKFKVGVREAFDAWYEQSRFGFSEVDQNEKADIKVSFEVGNHGDGYPFRKGSGVL 234
               P   +   R AF  W       F E +    ADIK+ F  G+HGDG PF    GVL
Sbjct: 170 PYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVL 229

Query: 235 AHAFAPVDGRLHFNADQSFSI----QVTYGMFHLRSVAIHELGHTLGLAHSTSQEAIMFP 294
           AH F+P +GRLH +  +++++    + +     L SVA+HE+GH LGL HS+ ++A M+P
Sbjct: 230 AHTFSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYP 289

Query: 295 SLPPNLSK-SLDMDDVNGLWDLY 307
           +L P   K +L+MDDV G+  LY
Sbjct: 290 TLKPRSKKVNLNMDDVVGVQSLY 295

[Close]

BLAST of Lsi02G015310 vs. NCBI nr
Match: gi|700197234|gb|KGN52411.1| (hypothetical protein Csa_5G632100 [Cucumis sativus])

HSP 1 Score: 437.6 bits (1124), Expect = 1.9e-119
Identity = 210/311 (67.52%), Postives = 256/311 (82.32%), Query Frame = 1


Query: 1   MYFKSSSLLLFLLLLVSITC-HPISARVLNHIHKSSQFLFPQYLLGSRKGHNIEGIHSIK 60
           M FKS  L LFLLLL  +   HPIS+R LNHIH  SQFLFP+YL+GSRKGHN+EG H+IK
Sbjct: 1   MDFKSLHLQLFLLLLTFLLLFHPISSRDLNHIHGPSQFLFPKYLIGSRKGHNLEGTHTIK 60

Query: 61  TYLQHYGYLNKNYNTIDTNGAYNNAFDDDLESSVKKYQKFFKLNESGILDVETLHRMSQP 120
           TYLQ YGYL+K+YN IDTNG YNNA+D+ LESS+KKYQKFF+LN+SGILD ETL +MSQ 
Sbjct: 61  TYLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMSQS 120

Query: 121 RCSVPDIFENEENETSVMTNNLHLRSKYTFFPGKPKWPSSKKYSLKYSFIRNFPEKFKVG 180
           RCSVPD FE+++NETS+ T+NLH+ S++ FFPG+PKWP SK YSL +SFI NFP  FK  
Sbjct: 121 RCSVPDFFESDDNETSMTTSNLHMGSRFKFFPGRPKWPDSKNYSLTFSFINNFPGIFKGE 180

Query: 181 VREAFDAWYEQSRFGFSEVDQNEKADIKVSFEVGNHGDGYPFRKGSGVLAHAFAPVDGRL 240
           V +AF AWYE+SRF F+EV + E++DIK+SFEVG+HGDG+PFR   GVLAHAF P DGRL
Sbjct: 181 VGDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPFR--VGVLAHAFTPTDGRL 240

Query: 241 HFNADQSFSIQVTYGMFHLRSVAIHELGHTLGLAHSTSQEAIMFPSLPPNLSKSLDMDDV 300
           HFN D+ FS +V  G +H+RSVA+HELGH+LGLAH+   +AIM+P+LPPN +KS++ DDV
Sbjct: 241 HFNGDEPFSSEVAEGKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAKSINSDDV 300

Query: 301 NGLWDLYDGFH 311
           NGLW LYD FH
Sbjct: 301 NGLWALYDNFH 309

[Close]

BLAST of Lsi02G015310 vs. NCBI nr
Match: gi|449449423|ref|XP_004142464.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 425.6 bits (1093), Expect = 7.3e-116
Identity = 209/311 (67.20%), Postives = 249/311 (80.06%), Query Frame = 1


Query: 1   MYFKSSS-LLLFLLLLVSITCHP-ISARVLNHIHKSSQFLFPQYLLGSRKGHNIEGIHSI 60
           M FKSSS L +FLLLL++    P I AR L+ I K SQ LFPQ+LLGS KGHNIEGIH+I
Sbjct: 1   MDFKSSSDLQIFLLLLLASIAFPFIWARDLHDI-KKSQLLFPQHLLGSSKGHNIEGIHTI 60

Query: 61  KTYLQHYGYLNKNYNTIDTNGAYNNAFDDDLESSVKKYQKFFKLNESGILDVETLHRMSQ 120
           K +LQ YGYL+KNYN IDTNGAYNNAFDD LES++KKYQ FFKL +SG+LD+ETLH+MSQ
Sbjct: 61  KMHLQRYGYLSKNYNIIDTNGAYNNAFDDHLESAIKKYQMFFKLPKSGVLDMETLHQMSQ 120

Query: 121 PRCSVPDIFENEENETSVMTNNLHLRSKYTFFPGKPKWPSSKKYSLKYSFIRNFPEKFKV 180
            RCSVPDIFEN ENETSV T+NLH+ SKYTFFPG+ KWP S  Y L Y+ + NFPE+FK 
Sbjct: 121 ARCSVPDIFENNENETSVTTSNLHIGSKYTFFPGRVKWPDSLNYRLTYALVNNFPEEFKE 180

Query: 181 GVREAFDAWYEQSRFGFSEVDQNEKADIKVSFEVGNHGDGYPFRKGSGVLAHAFAPVDGR 240
            VR AF+ WY +SRF F+EV +NE  +I++SFE G HGD +PF K S  LAH FAP+DGR
Sbjct: 181 SVRTAFEIWYGRSRFNFTEVSENEGGNIRISFERGVHGDYHPFTKNSKTLAHTFAPIDGR 240

Query: 241 LHFNADQSFSIQVTYGMFHLRSVAIHELGHTLGLAHSTSQEAIMFPSLPPNLSKSLDMDD 300
            HFNAD+ FS+ VTY  +HLR+VA+HELGH  GLAHS S++AIMFP++P NL K LDMDD
Sbjct: 241 FHFNADKPFSVDVTYNAYHLRTVALHELGHAFGLAHSPSEDAIMFPTIPTNLEKDLDMDD 300

Query: 301 VNGLWDLYDGF 310
           + GLW+LYDGF
Sbjct: 301 IEGLWELYDGF 310

[Close]

BLAST of Lsi02G015310 vs. NCBI nr
Match: gi|659118021|ref|XP_008458908.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 420.6 bits (1080), Expect = 2.4e-114
Identity = 204/306 (66.67%), Postives = 247/306 (80.72%), Query Frame = 1


Query: 1   MYFKSSSLLLFLLLLVSITCHPISARVLNHIHKSSQFLFPQYLLGSRKGHNIEGIHSIKT 60
           M FKS  L LFLLL      HPIS+R LNHIH  SQFLFP+YLLGSRKGHNIEG H+IKT
Sbjct: 1   MDFKSFYLQLFLLLTSLSLFHPISSRDLNHIHGPSQFLFPKYLLGSRKGHNIEGTHAIKT 60

Query: 61  YLQHYGYLNKNYNTIDTNGAYNNAFDDDLESSVKKYQKFFKLNESGILDVETLHRMSQPR 120
           YLQHYGYL+KNYN IDTNG YNNA+D+ LE SVKKYQKFFKLNESGILD ETL +MSQPR
Sbjct: 61  YLQHYGYLSKNYNIIDTNGVYNNAYDEQLECSVKKYQKFFKLNESGILDRETLSQMSQPR 120

Query: 121 CSVPDIFENEENETSVMTNNLHLRSKYTFFPGKPKWPSSKKYSLKYSFIRNFPEKFKVGV 180
           CSVPDIFE+E+NETS+ T+NLH+RS+Y FFPG+PKW  S+  +L YS I+NFPEKF+  +
Sbjct: 121 CSVPDIFESEDNETSITTSNLHIRSRYAFFPGRPKWSDSR--NLTYSLIKNFPEKFEAAI 180

Query: 181 REAFDAWYEQSRFGFSEVDQNEKADIKVSFEVGNHGDGYPFRKGSGVLAHAFAPVDGRLH 240
            +AF  W+ +SRF F+ V + +K+DI +SFEVG+HGDG+PF+   GVLAHAF+P DGRLH
Sbjct: 181 VDAFMLWFLRSRFCFTYVSEGQKSDITISFEVGDHGDGHPFK--GGVLAHAFSPEDGRLH 240

Query: 241 FNADQSFSIQVTYGMFHLRSVAIHELGHTLGLAHSTSQEAIMFPSLPPNLSKSLDMDDVN 300
           FN D+SFS  +  G +H+R+VA HELGH+LGLAH+  + AIM+P+LP N SK L  DDV+
Sbjct: 241 FNGDKSFSSDLEEGKYHIRTVAAHELGHSLGLAHTNIEAAIMYPTLPSNFSKILSQDDVD 300

Query: 301 GLWDLY 307
           GLW+ Y
Sbjct: 301 GLWNFY 302

[Close]

BLAST of Lsi02G015310 vs. NCBI nr
Match: gi|778708743|ref|XP_011656274.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 277.3 bits (708), Expect = 3.2e-71
Identity = 127/195 (65.13%), Postives = 159/195 (81.54%), Query Frame = 1


Query: 116 MSQPRCSVPDIFENEENETSVMTNNLHLRSKYTFFPGKPKWPSSKKYSLKYSFIRNFPEK 175
           MSQ RCSVPD FE+++NETS+ T+NLH+ S++ FFPG+PKWP SK YSL +SFI NFP  
Sbjct: 1   MSQSRCSVPDFFESDDNETSMTTSNLHMGSRFKFFPGRPKWPDSKNYSLTFSFINNFPGI 60

Query: 176 FKVGVREAFDAWYEQSRFGFSEVDQNEKADIKVSFEVGNHGDGYPFRKGSGVLAHAFAPV 235
           FK  V +AF AWYE+SRF F+EV + E++DIK+SFEVG+HGDG+PFR   GVLAHAF P 
Sbjct: 61  FKGEVGDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPFR--VGVLAHAFTPT 120

Query: 236 DGRLHFNADQSFSIQVTYGMFHLRSVAIHELGHTLGLAHSTSQEAIMFPSLPPNLSKSLD 295
           DGRLHFN D+ FS +V  G +H+RSVA+HELGH+LGLAH+   +AIM+P+LPPN +KS++
Sbjct: 121 DGRLHFNGDEPFSSEVAEGKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAKSIN 180

Query: 296 MDDVNGLWDLYDGFH 311
            DDVNGLW LYD FH
Sbjct: 181 SDDVNGLWALYDNFH 193

[Close]

BLAST of Lsi02G015310 vs. NCBI nr
Match: gi|659118035|ref|XP_008458917.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 256.1 bits (653), Expect = 7.7e-65
Identity = 144/306 (47.06%), Postives = 184/306 (60.13%), Query Frame = 1


Query: 1   MYFKSSSLLLFLLLLVSITCHPISARVLNHIHKSSQFLFPQYLLGSRKGHNIEGIHSIKT 60
           M FKS  L LF LLL S T H IS  +   I        PQ+L G RKG N+EGIHSIK 
Sbjct: 1   MAFKS--LQLFFLLLASFTSHVISRHIPTPI--------PQHLYGCRKGDNVEGIHSIKK 60

Query: 61  YLQHYGYLNKNYNTIDTNGAYNNAFDDDLESSVKKYQKFFKLNESGILDVETLHRMSQPR 120
           YL  YGYL+ N NT       +N FDD LES+VK YQK+ KLN SGILD +TL ++ +PR
Sbjct: 61  YLHRYGYLSHNTNTSSHIELNSNTFDDTLESAVKLYQKWSKLNVSGILDQQTLDQIFKPR 120

Query: 121 CSVPDIFENEENETSVMTNNLHLRSKYTFFPGKPKWPSSKKYSLKYSFIRNFPEKFKVGV 180
           C +PD+   + N      ++L + S Y  FPG  KWP  K++ L Y+F  NFP  F   V
Sbjct: 121 CGLPDVIM-KSNSNKNTEDDLEISSHYALFPGNQKWPDYKRH-LTYTFTNNFPIDFVPSV 180

Query: 181 REAFDAWYEQSRFGFSEVDQNEKADIKVSFEVGNHGDGYPFRKGSGVLAHAFAPVDGRLH 240
            EA   W   S F FS+   ++  DI +SF+VG+HGDG PF    GV+ HAFAP DGRLH
Sbjct: 181 TEAMKEWAAHSLFTFSQAADDQVPDINISFQVGDHGDGVPFDGPGGVVGHAFAPTDGRLH 240

Query: 241 FNADQSFSIQVTYGMFHLRSVAIHELGHTLGLAHSTSQEAIMFPSLPPNLSKSLDMDDVN 300
            + D  +S  +     ++ + A+HELGH LGLAHST Q+A+M+P +     K+L  DD+ 
Sbjct: 241 LDGDDKWSAGLETQKVNVMNAALHELGHVLGLAHSTVQQAVMWPYIDVYALKALTDDDIA 294

Query: 301 GLWDLY 307
           GL  LY
Sbjct: 301 GLTALY 294

The following BLAST results are available for this feature:

BLAST of Lsi02G015310 vs. Swiss-Prot


Match Name	E-value	Identity	Description
MEP1_SOYBN	1.0e-45	41.20	Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2	[more]
5MMP_ARATH	6.3e-43	35.49	Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1	[more]
2MMP_ARATH	5.4e-42	34.88	Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1	[more]
1MMP_ARATH	2.7e-41	37.67	Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1	[more]
3MMP_ARATH	3.6e-38	31.95	Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1	[more]

BLAST of Lsi02G015310 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KX75_CUCSA	1.3e-119	67.52	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632100 PE=3 SV=1	[more]
A0A0A0KS15_CUCSA	5.1e-116	67.20	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632090 PE=3 SV=1	[more]
A0A0A0KNY8_CUCSA	1.6e-64	47.40	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G522910 PE=3 SV=1	[more]
A0A0A0KS35_CUCSA	5.9e-64	46.77	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632070 PE=3 SV=1	[more]
A0A0A0KU02_CUCSA	8.6e-63	48.01	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632080 PE=3 SV=1	[more]

BLAST of Lsi02G015310 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G59970.1	3.6e-44	35.49	Matrixin family protein	[more]
AT1G70170.1	3.0e-43	34.88	matrix metalloproteinase	[more]
AT4G16640.1	1.5e-42	37.67	Matrixin family protein	[more]
AT1G24140.1	2.0e-39	31.95	Matrixin family protein	[more]
AT2G45040.1	4.2e-37	36.12	Matrixin family protein	[more]

BLAST of Lsi02G015310 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700197234\|gb\|KGN52411.1\|	1.9e-119	67.52	hypothetical protein Csa_5G632100 [Cucumis sativus]	[more]
gi\|449449423\|ref\|XP_004142464.1\|	7.3e-116	67.20	PREDICTED: metalloendoproteinase 1-like [Cucumis sativus]	[more]
gi\|659118021\|ref\|XP_008458908.1\|	2.4e-114	66.67	PREDICTED: metalloendoproteinase 1-like [Cucumis melo]	[more]
gi\|778708743\|ref\|XP_011656274.1\|	3.2e-71	65.13	PREDICTED: metalloendoproteinase 1-like [Cucumis sativus]	[more]
gi\|659118035\|ref\|XP_008458917.1\|	7.7e-65	47.06	PREDICTED: metalloendoproteinase 1-like [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0008237	metallopeptidase activity
GO:0008270	zinc ion binding
GO:0004222	metalloendopeptidase activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0031012	extracellular matrix

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

Vocabulary: **INTERPRO**
Term	Definition
IPR024079	MetalloPept_cat_dom_sf
IPR021190	Pept_M10A
IPR006026	Peptidase_Metallo
IPR002477	Peptidoglycan-bd-like
IPR001818	Pept_M10_metallopeptidase

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0031012	extracellular matrix
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0008237	metallopeptidase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G015310.1	Lsi02G015310.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 174..306 score: 1.5
IPR002477	Peptidoglycan binding-like	GENE3D	G3DSA:1.10.101.10		coord: 54..118 score: 8.8
IPR002477	Peptidoglycan binding-like	PFAM	PF01471	PG_binding_1	coord: 53..116 score: 3.5
IPR002477	Peptidoglycan binding-like	unknown	SSF47090	PGBD-like	coord: 46..126 score: 5.81
IPR006026	Peptidase, metallopeptidase	SMART	SM00235	col_5	coord: 152..308 score: 1.6
IPR021190	Peptidase M10A	PRINTS	PR00138	MATRIXIN	coord: 180..195 score: 6.7E-32coord: 204..232 score: 6.7E-32coord: 294..307 score: 6.7E-32coord: 261..286 score: 6.7E-32coord: 116..129 score: 6.7
IPR024079	Metallopeptidase, catalytic domain	GENE3D	G3DSA:3.40.390.10		coord: 119..307 score: 6.2
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 40..306 score: 1.8E-89coord: 2..23 score: 1.8
None	No IPR available	PANTHER	PTHR10201:SF152	SUBFAMILY NOT NAMED	coord: 40..306 score: 1.8E-89coord: 2..23 score: 1.8
None	No IPR available	unknown	SSF55486	Metalloproteases ("zincins"), catalytic domain	coord: 118..306 score: 1.47

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi02G015310	CSPI05G28520	Wild cucumber (PI 183967)	cpilsiB352
Lsi02G015310	Cucsa.158770	Cucumber (Gy14) v1	cgylsiB251
Lsi02G015310	Cla010371	Watermelon (97103) v1	lsiwmB170
Lsi02G015310	MELO3C021497	Melon (DHL92) v3.5.1	lsimeB162
Lsi02G015310	ClCG09G018610	Watermelon (Charleston Gray)	lsiwcgB155
Lsi02G015310	MELO3C021493.2	Melon (DHL92) v3.6.1	lsimedB171
Lsi02G015310	Cla97C09G180060	Watermelon (97103) v2	lsiwmbB208
Lsi02G015310	Bhi06G000390	Wax gourd	lsiwgoB207
Lsi02G015310	CsGy5G027770	Cucumber (Gy14) v2	cgyblsiB319
Lsi02G015310	CsaV3_5G037540	Cucumber (Chinese Long) v3	cuclsiB371

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi02G015310	Cucurbita pepo (Zucchini)	cpelsiB141
Lsi02G015310	Cucurbita pepo (Zucchini)	cpelsiB336
Lsi02G015310	Wild cucumber (PI 183967)	cpilsiB028
Lsi02G015310	Cucumber (Chinese Long) v2	culsiB028
Lsi02G015310	Cucumber (Chinese Long) v2	culsiB348
Lsi02G015310	Melon (DHL92) v3.5.1	lsimeB182
Lsi02G015310	Watermelon (Charleston Gray)	lsiwcgB176
Lsi02G015310	Cucumber (Gy14) v2	cgyblsiB024
Lsi02G015310	Melon (DHL92) v3.6.1	lsimedB191
Lsi02G015310	Silver-seed gourd	carlsiB232
Lsi02G015310	Silver-seed gourd	carlsiB623
Lsi02G015310	Cucumber (Chinese Long) v3	cuclsiB028
Lsi02G015310	Watermelon (97103) v2	lsiwmbB161
Lsi02G015310	Wax gourd	lsiwgoB253
Lsi02G015310	Bottle gourd (USVL1VR-Ls)	lsilsiB089
Lsi02G015310	Cucumber (Gy14) v1	cgylsiB118
Lsi02G015310	Cucurbita maxima (Rimu)	cmalsiB272
Lsi02G015310	Cucurbita maxima (Rimu)	cmalsiB372
Lsi02G015310	Cucurbita maxima (Rimu)	cmalsiB651
Lsi02G015310	Cucurbita moschata (Rifu)	cmolsiB261
Lsi02G015310	Cucurbita moschata (Rifu)	cmolsiB640