Lsi02G015270 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi02G015270
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Matrix metalloproteinase
Location	chr02 : 21156249 .. 21157589 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTTATTACACACAACTAGAGCCTTTATTGGAGAGAGCCATGGCAATTAAAGGATTATCATTCTTCATTTCCTCTTTTTTTCTTCTAATTATCCTTTTCCCTTTAATATCATCTTCTCAGCCAAACACTGCTCATTCTCATTCTCATGGATTGGTGTTTCTAAACAACCTCAATGGATGCAAGAAAGGTGACAAAGTAGAAGGAATCCACCAAATGAAGGAATATCTTCAGCATTTTGGCTACTTGAACCATGTTCAAAATCATTCTAAATCTTTTAATGATGATGAGTACGACGAGCTCTTAGAGTCCGCTATTAAAACTTACCAACTCAACTACAATCTCAAGGCCACTGGTGCTCTCGATGCCACAACACTAGCTCTTATGTCGAAACCTCGATGCGGGGTTGCGGACATCGTCGACGGAAAGACGAGGATGAAATCAGGCAAGAAAATGGTGAATCAACATAGAAAAATAAATGGTCATTTTCATACCGTTTCCCACTATGCTTTCTTCGAAGGAAATCCTAAATGGCCTGCTTCTAAATCCCACCTCACTTATGGTAAATTTAATCTTTTATTATTCATATAGTTTACCTACATTCGTCACCCTATTATAAGTTTAAACTTATGAGCTATAGTATTATAGAAGTTTGATTATAACAGGATCTTCTACTTTGATACTATGTTAATTTTACCGTTGAATTCAAAGGTTTAACCTTTTATTCACATTCTTAATAGGATTCCTCCCGGGAACCCCATCTGAGGCGATAGCTCCGGTGGGTCGAGCTTTTACGACGTGGGCTGCGAACACTCACTTCAGCTTCTCCCAAGCATCTGGATATGAAACTGCTGACATCAAAATAAGCTTTGAAAGTGGGGACCATGGAGATGGCCATGCCTTCGACGGCGTTGGAGGAGTCATAGCTCATGCCTTTTCACCCACAGATGGGAGACTGCATTTCGATGCCGTCGAGCCTTGGGCCGTCGGTGCCGTTGATGATTCGTTTGACGTGGAGACGGTGGCATTGCATGAGATCGGACACCTTCTCGGTCTCCATCACAGCTCCGTGGAAGGGGCTATTATGTGGCCAACCATCATGGGAGGAGCTACTAAGGGTTTGCATGCCGACGACATTGCAGGCATTAATGCATTATACACTGCTACTTCTTGAGTAGTGTTAGAGCTATTCTTTCCTCAATTTGTTATTTCGTTTTTACAATAGTTGCTATTTGTTTTTACATGTAACTTAATTCAAATTATTATTTTCATTTTTCAATGAATATGTGTATTCGTCAACTATAACAGCTTATAGTATATGTGATTCTATGAAAGGTTTATAA

mRNA sequence

ATTTTATTACACACAACTAGAGCCTTTATTGGAGAGAGCCATGGCAATTAAAGGATTATCATTCTTCATTTCCTCTTTTTTTCTTCTAATTATCCTTTTCCCTTTAATATCATCTTCTCAGCCAAACACTGCTCATTCTCATTCTCATGGATTGGTGTTTCTAAACAACCTCAATGGATGCAAGAAAGGTGACAAAGTAGAAGGAATCCACCAAATGAAGGAATATCTTCAGCATTTTGGCTACTTGAACCATGTTCAAAATCATTCTAAATCTTTTAATGATGATGAGTACGACGAGCTCTTAGAGTCCGCTATTAAAACTTACCAACTCAACTACAATCTCAAGGCCACTGGTGCTCTCGATGCCACAACACTAGCTCTTATGTCGAAACCTCGATGCGGGGTTGCGGACATCGTCGACGGAAAGACGAGGATGAAATCAGGCAAGAAAATGGTGAATCAACATAGAAAAATAAATGGTCATTTTCATACCGTTTCCCACTATGCTTTCTTCGAAGGAAATCCTAAATGGCCTGCTTCTAAATCCCACCTCACTTATGGATTCCTCCCGGGAACCCCATCTGAGGCGATAGCTCCGGTGGGTCGAGCTTTTACGACGTGGGCTGCGAACACTCACTTCAGCTTCTCCCAAGCATCTGGATATGAAACTGCTGACATCAAAATAAGCTTTGAAAGTGGGGACCATGGAGATGGCCATGCCTTCGACGGCGTTGGAGGAGTCATAGCTCATGCCTTTTCACCCACAGATGGGAGACTGCATTTCGATGCCGTCGAGCCTTGGGCCGTCGGTGCCGTTGATGATTCGTTTGACGTGGAGACGGTGGCATTGCATGAGATCGGACACCTTCTCGGTCTCCATCACAGCTCCGTGGAAGGGGCTATTATGTGGCCAACCATCATGGGAGGAGCTACTAAGGGTTTGCATGCCGACGACATTGCAGGCATTAATGCATTATACACTGCTACTTCTTGAGTAGTGTTAGAGCTATTCTTTCCTCAATTTGTTATTTCGTTTTTACAATAGTTGCTATTTGTTTTTACATGTAACTTAATTCAAATTATTATTTTCATTTTTCAATGAATATGTGTATTCGTCAACTATAACAGCTTATAGTATATGTGATTCTATGAAAGGTTTATAA

Coding sequence (CDS)

ATGGCAATTAAAGGATTATCATTCTTCATTTCCTCTTTTTTTCTTCTAATTATCCTTTTCCCTTTAATATCATCTTCTCAGCCAAACACTGCTCATTCTCATTCTCATGGATTGGTGTTTCTAAACAACCTCAATGGATGCAAGAAAGGTGACAAAGTAGAAGGAATCCACCAAATGAAGGAATATCTTCAGCATTTTGGCTACTTGAACCATGTTCAAAATCATTCTAAATCTTTTAATGATGATGAGTACGACGAGCTCTTAGAGTCCGCTATTAAAACTTACCAACTCAACTACAATCTCAAGGCCACTGGTGCTCTCGATGCCACAACACTAGCTCTTATGTCGAAACCTCGATGCGGGGTTGCGGACATCGTCGACGGAAAGACGAGGATGAAATCAGGCAAGAAAATGGTGAATCAACATAGAAAAATAAATGGTCATTTTCATACCGTTTCCCACTATGCTTTCTTCGAAGGAAATCCTAAATGGCCTGCTTCTAAATCCCACCTCACTTATGGATTCCTCCCGGGAACCCCATCTGAGGCGATAGCTCCGGTGGGTCGAGCTTTTACGACGTGGGCTGCGAACACTCACTTCAGCTTCTCCCAAGCATCTGGATATGAAACTGCTGACATCAAAATAAGCTTTGAAAGTGGGGACCATGGAGATGGCCATGCCTTCGACGGCGTTGGAGGAGTCATAGCTCATGCCTTTTCACCCACAGATGGGAGACTGCATTTCGATGCCGTCGAGCCTTGGGCCGTCGGTGCCGTTGATGATTCGTTTGACGTGGAGACGGTGGCATTGCATGAGATCGGACACCTTCTCGGTCTCCATCACAGCTCCGTGGAAGGGGCTATTATGTGGCCAACCATCATGGGAGGAGCTACTAAGGGTTTGCATGCCGACGACATTGCAGGCATTAATGCATTATACACTGCTACTTCTTGA

Protein sequence

MAIKGLSFFISSFFLLIILFPLISSSQPNTAHSHSHGLVFLNNLNGCKKGDKVEGIHQMKEYLQHFGYLNHVQNHSKSFNDDEYDELLESAIKTYQLNYNLKATGALDATTLALMSKPRCGVADIVDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFEGNPKWPASKSHLTYGFLPGTPSEAIAPVGRAFTTWAANTHFSFSQASGYETADIKISFESGDHGDGHAFDGVGGVIAHAFSPTDGRLHFDAVEPWAVGAVDDSFDVETVALHEIGHLLGLHHSSVEGAIMWPTIMGGATKGLHADDIAGINALYTATS

Homology

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BLAST of Lsi02G015270 vs. Swiss-Prot
Match: 3MMP_ARATH (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.2e-60
Identity = 127/285 (44.56%), Postives = 169/285 (59.30%), Query Frame = 1


Query: 43  NLNGCKKGDKVEGIHQMKEYLQHFGYLNHVQNHSKSFNDDEYDELLESAIKTYQLNYNLK 102
           N  GC  G K +G++ +K+Y QHFGY+    N S +F DD +D++L++A++ YQ N+ L 
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITET-NLSGNFTDD-FDDILKNAVEMYQRNFQLN 107

Query: 103 ATGALDATTLALMSKPRCGVADIVDGKTRMKSGKKMVNQHRKINGH-FHTVSHYAFFEGN 162
            TG LD  TL  +  PRCG  D+V+G + M SG+K         G  FH V HY+FF G 
Sbjct: 108 VTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGE 167

Query: 163 PKWPASKSHLTYGFLP--GTPSEAIAPVGRAFTTWAANTHFSFSQASGYETADIKISFES 222
           P+WP ++  LTY F P      E  +   RAFT W   T  +F++   + T+DI I F S
Sbjct: 168 PRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYS 227

Query: 223 GDHGDGHAFDGVGGVIAHAFSPTDGRLHFDAVEPWAVG--------AVDDSFDVETVALH 282
           G+HGDG  FDG    +AHAFSP  G  H D  E W V         +V ++ D+E+VA+H
Sbjct: 228 GEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVH 287

Query: 283 EIGHLLGLHHSSVEGAIMWPTIMGGATK-GLHADDIAGINALYTA 316
           EIGHLLGL HSSVEG+IM+PTI  G  K  L  DD+ G+  LY A
Sbjct: 288 EIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYGA 330

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BLAST of Lsi02G015270 vs. Swiss-Prot
Match: 2MMP_ARATH (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.3e-59
Identity = 133/323 (41.18%), Postives = 185/323 (57.28%), Query Frame = 1


Query: 8   FFISSFFLLII-----LFPLISSSQPNTAHSHSHGLVFLNNLNGCKKGDKVEGIHQMKEY 67
           F   S FL++       FP  ++  P+  ++        +N  GC  G  V+G++++K+Y
Sbjct: 6   FGFLSLFLIVSPASAWFFPNSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKKY 65

Query: 68  LQHFGYLNHVQNHSKSFNDDEYDELLESAIKTYQLNYNLKATGALDATTLALMSKPRCGV 127
            Q FGY+   +  S +F DD +D++L++A++ YQ N+NL  TG LDA T+  +  PRCG 
Sbjct: 66  FQRFGYIP--ETFSGNFTDD-FDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGN 125

Query: 128 ADIVDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFEGNPKWPASKSHLTYGFLPGTP-- 187
            D+V+G + M  G++   +      H H V  Y  F G P+WP ++  LTY F P  P  
Sbjct: 126 PDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLT 185

Query: 188 SEAIAPVGRAFTTWAANTHFSFSQASGYETADIKISFESGDHGDGHAFDGVGGVIAHAFS 247
            E  +   RAF  W+  T  +F+ +  + T+DI I F +GDHGDG  FDGV G +AHAFS
Sbjct: 186 EEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFS 245

Query: 248 PTDGRLHFDAVEPWAVGAVDDSF-------DVETVALHEIGHLLGLHHSSVEGAIMWPTI 307
           P  G+ H DA E W V    DSF       D+E+VA+HEIGHLLGL HSSVE +IM+PTI
Sbjct: 246 PPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTI 305

Query: 308 MGGATK-GLHADDIAGINALYTA 316
             G  K  L  DD+ GI  LY A
Sbjct: 306 TTGKRKVDLTNDDVEGIQYLYGA 325

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BLAST of Lsi02G015270 vs. Swiss-Prot
Match: 5MMP_ARATH (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.3e-56
Identity = 125/284 (44.01%), Postives = 161/284 (56.69%), Query Frame = 1


Query: 42  NNLNGCKKGDKVEGIHQMKEYLQHFGYLNHVQNHSKSFNDDEYDELLESAIKTYQLNYNL 101
           + L GC  G+ + G+ ++K+Y + FGY+    N +     D++D++L+SAI TYQ N+NL
Sbjct: 46  SKLAGCHIGENINGLSKLKQYFRRFGYITTTGNCT-----DDFDDVLQSAINTYQKNFNL 105

Query: 102 KATGALDATTLALMSKPRCGVADIVDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFEGN 161
           K TG LD++TL  + KPRCG  D++DG + M  GK +            T   Y+FF G 
Sbjct: 106 KVTGKLDSSTLRQIVKPRCGNPDLIDGVSEMNGGKIL-----------RTTEKYSFFPGK 165

Query: 162 PKWPASKSHLTYGFLP--GTPSEAIAPVGRAFTTWAANTHFSFSQASGYETADIKISFES 221
           P+WP  K  LTY F P      E      RAFT WA  T  +F+++     ADI I F S
Sbjct: 166 PRWPKRKRDLTYAFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFS 225

Query: 222 GDHGDGHAFDGVGGVIAHAFSPTDGRLHFDAVEPWAVG---------AVDDSFDVETVAL 281
           G+HGDG  FDG  G +AHA SP  G LH D  E W +           V    D+E+VA+
Sbjct: 226 GEHGDGEPFDGAMGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAV 285

Query: 282 HEIGHLLGLHHSSVEGAIMWPTIMGGATK-GLHADDIAGINALY 314
           HEIGHLLGL HSSVE AIM+P I GG  K  L  DDI GI  LY
Sbjct: 286 HEIGHLLGLGHSSVEDAIMFPAISGGDRKVELAKDDIEGIQHLY 313

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BLAST of Lsi02G015270 vs. Swiss-Prot
Match: MEP1_SOYBN (Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2)

HSP 1 Score: 204.1 bits (518), Expect = 2.2e-51
Identity = 116/273 (42.49%), Postives = 151/273 (55.31%), Query Frame = 1


Query: 50  GDKVEGIHQMKEYLQHFGYLNHVQNHSKSFNDDEYDELLESAIKTYQLNYNLKATGALDA 109
           G   +G+  +K Y  H GY+ +  +      DD +D+ L SAIKTYQ NYNL  TG  D 
Sbjct: 48  GQNYKGLSNVKNYFHHLGYIPNAPHF-----DDNFDDTLVSAIKTYQKNYNLNVTGKFDI 107

Query: 110 TTLALMSKPRCGVADIVDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFEGNPKWPASKS 169
            TL  +  PRCGV DI+    +  S              F  +S Y FF+  P+W A  +
Sbjct: 108 NTLKQIMTPRCGVPDIIINTNKTTS--------------FGMISDYTFFKDMPRWQAGTT 167

Query: 170 HLTYGFLPGTPSEAI--APVGRAFTTWAANTHFSFSQASGYETADIKISFESGDHGDGHA 229
            LTY F P    +    + + RAF+ W    + +F + + YETA+IKI F S +HGD + 
Sbjct: 168 QLTYAFSPEPRLDDTFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYP 227

Query: 230 FDGVGGVIAHAFSPTDGRLHFDAVEPWAVGA------VDDSFDVETVALHEIGHLLGLHH 289
           FDG GG++ HAF+PTDGR HFDA E W          V  +FD+E+VA+HEIGHLLGL H
Sbjct: 228 FDGPGGILGHAFAPTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGH 287

Query: 290 SSVEGAIMWPTIMGGATK-GLHADDIAGINALY 314
           SS   AIM+P+I     K  L  DDI GI  LY
Sbjct: 288 SSDLRAIMYPSIPPRTRKVNLAQDDIDGIRKLY 301

[Close]

BLAST of Lsi02G015270 vs. Swiss-Prot
Match: 1MMP_ARATH (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 1.4e-50
Identity = 123/277 (44.40%), Postives = 160/277 (57.76%), Query Frame = 1


Query: 50  GDKVEGIHQMKEYLQHFGYLNHVQNHSKSFNDDEYDELLESAIKTYQLNYNLKATGALDA 109
           G  V G+ ++K YL  FGY+N   + S+ F+D  +D  LESAI  YQ N  L  TG LD 
Sbjct: 64  GSHVSGVSELKRYLHRFGYVN---DGSEIFSD-VFDGPLESAISLYQENLGLPITGRLDT 123

Query: 110 TTLALMSKPRCGVADIVDGKTRMKSGKKMVNQHRKINGHF-HTVSHYAFFEGNPKWPASK 169
           +T+ LMS PRCGV+D                 H  IN  F HT +HY +F G PKW  ++
Sbjct: 124 STVTLMSLPRCGVSDT----------------HMTINNDFLHTTAHYTYFNGKPKW--NR 183

Query: 170 SHLTYGF-----LPGTPSEAIAPV-GRAFTTWAANTHFSFSQASGYETADIKISFESGDH 229
             LTY       L    SE +  V  RAF+ W++    SF +   + TAD+KI F +GDH
Sbjct: 184 DTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDH 243

Query: 230 GDGHAFDGVGGVIAHAFSPTDGRLHFDAVEPWAV-----GAVDDSFDVETVALHEIGHLL 289
           GDG  FDGV G +AHAF+P +GRLH DA E W V     G+ + + D+E+VA HEIGHLL
Sbjct: 244 GDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLL 303

Query: 290 GLHHSSVEGAIMWPTIMGGATK-GLHADDIAGINALY 314
           GL HSS E A+M+P++     K  L  DD+AG+  LY
Sbjct: 304 GLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]

BLAST of Lsi02G015270 vs. TrEMBL
Match: A0A0A0KU08_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632130 PE=3 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 9.4e-142
Identity = 251/312 (80.45%), Postives = 281/312 (90.06%), Query Frame = 1


Query: 6   LSFFISSFFLLIILFPLISSSQPNTAHSH-SHGLVFLNNLNGCKKGDKVEGIHQMKEYLQ 65
           LSF  +SFFL+I+LFPLISSS P    SH S+GLVFL NLNGCKKGDKVEGIHQ+KEYLQ
Sbjct: 7   LSFLTTSFFLVIVLFPLISSSHPK---SHLSNGLVFLKNLNGCKKGDKVEGIHQVKEYLQ 66

Query: 66  HFGYLN-HVQNHSKSFNDDEYDELLESAIKTYQLNYNLKATGALDATTLALMSKPRCGVA 125
           HFGYLN +VQN+SKSF+DDE+D +LESAIKTYQLNYNLKATG ++A TL LMS+PRCG A
Sbjct: 67  HFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCGFA 126

Query: 126 DIVDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFEGNPKWPASKSHLTYGFLPGTPSEA 185
           DIVDGKTRMKSGKKMVNQHRKINGHFH+VSHYAFF+GNPKWPASKSHLTYGFLPGTPS+A
Sbjct: 127 DIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTPSKA 186

Query: 186 IAPVGRAFTTWAANTHFSFSQASGYETADIKISFESGDHGDGHAFDGVGGVIAHAFSPTD 245
           +A +GRAF TW ANTHF+FSQ S Y+ ADIKISFE+GDHGDGH+FDGV GVIAH F+P D
Sbjct: 187 VATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFAPRD 246

Query: 246 GRLHFDAVEPWAVGAVDDSFDVETVALHEIGHLLGLHHSSVEGAIMWPTIMGGATKGLHA 305
           GRLHFDA E WAVGA+ DSFD+ET+ALHEIGHLLGL+HSS+E AIMWP+IM G+TKGL A
Sbjct: 247 GRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKGLDA 306

Query: 306 DDIAGINALYTA 316
           DDIAGINALY A
Sbjct: 307 DDIAGINALYNA 315

[Close]

BLAST of Lsi02G015270 vs. TrEMBL
Match: A0A0A0KS47_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G633160 PE=3 SV=1)

HSP 1 Score: 485.0 bits (1247), Expect = 7.2e-134
Identity = 241/317 (76.03%), Postives = 266/317 (83.91%), Query Frame = 1


Query: 1   MAIKGLS-FFISSFFLLIILFPLISSSQPNTAHSHSHGLVFLNNLNGCKKGDKVEGIHQM 60
           M  +GLS FF  + FL+I LFPLISSSQP        GLVFL NL GCKKGDKVEGIHQM
Sbjct: 1   MGFQGLSLFFTFTLFLIISLFPLISSSQPKPVD----GLVFLKNLKGCKKGDKVEGIHQM 60

Query: 61  KEYLQHFGYLNHVQNHSKSFNDDEYDELLESAIKTYQLNYNLKATGALDATTLALMSKPR 120
           K+YLQHFGYLN+  +     +DDE+DE+LE A+KTYQ+NYNLK TG LD +TL  MSKPR
Sbjct: 61  KKYLQHFGYLNNDVHIRSESSDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKPR 120

Query: 121 CGVADIVDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFEGNPKWPASKSHLTYGFLPGT 180
           CGVADI++GKT MKSGK+ VNQHRKI+GHFH VSH+AFFEGN +WP SKSHLTYGFLPGT
Sbjct: 121 CGVADIINGKTSMKSGKRFVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGFLPGT 180

Query: 181 PSEAIAPVGRAFTTWAANTHFSFSQASGYETADIKISFESGDHGDGHAFDGVGGVIAHAF 240
           PSEAI+PV RAFTTWAANTHFSFSQ S YE ADIKISFE GDHGDG  FD VGGV+AHAF
Sbjct: 181 PSEAISPVNRAFTTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVLAHAF 240

Query: 241 SPTDGRLHFDAVEPWAVGAVDDSFDVETVALHEIGHLLGLHHSSVEGAIMWPTIMGGATK 300
           +PTDGRLHFDAVE WA GAV  S+D+ETVALHEIGHLLGLHHSSVEGAIMWP+IMGGATK
Sbjct: 241 APTDGRLHFDAVEHWADGAVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIMGGATK 300

Query: 301 GLHADDIAGINALYTAT 317
           GLHADDI GI  LYT +
Sbjct: 301 GLHADDIEGIKVLYTTS 313

[Close]

BLAST of Lsi02G015270 vs. TrEMBL
Match: A0A0A0KS39_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632120 PE=3 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 6.5e-119
Identity = 222/319 (69.59%), Postives = 253/319 (79.31%), Query Frame = 1


Query: 1   MAIKGLSFFISSFFLLIILFPLISSSQPNTAHSHSHG-----LVFLNNLNGCKKGDKVEG 60
           MA++ LSFF  +  LL+  FPLISSSQ N  HSHSHG      VFLN L G +KGDKVEG
Sbjct: 1   MALEELSFFTFTL-LLLTFFPLISSSQSN--HSHSHGKASSQFVFLNQLYGSQKGDKVEG 60

Query: 61  IHQMKEYLQHFGYLNH-VQNHSKSFNDDEYDELLESAIKTYQLNYNLKATGALDATTLAL 120
           IHQ+K+YL  FGYLN   Q HS++ NDDE+DELLE+AIKTYQ NYNLK TG LDA T+A 
Sbjct: 61  IHQLKKYLHQFGYLNEDAQIHSEN-NDDEFDELLETAIKTYQKNYNLKVTGTLDAMTIAQ 120

Query: 121 MSKPRCGVADIVDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFEGNPKWPASKSHLTYG 180
           MSKPRCGVADI+ G T M+S KK       I GHFH+VSH+AFF+GNPKWPA+KSHLTYG
Sbjct: 121 MSKPRCGVADIIHGNTWMRSSKKRKQLEHGIVGHFHSVSHFAFFDGNPKWPATKSHLTYG 180

Query: 181 FLPGTPSEAIAPVGRAFTTWAANTHFSFSQASGYETADIKISFESGDHGDGHAFDGVGGV 240
           FLPGTPSE ++PV RAF TWAAN+HF+FSQA   +T+DI I FE+GDHGDG+ FDGVGGV
Sbjct: 181 FLPGTPSETVSPVARAFATWAANSHFTFSQALDNQTSDITIGFETGDHGDGYPFDGVGGV 240

Query: 241 IAHAFSPTDGRLHFDAVEPWAVGAVDDSFDVETVALHEIGHLLGLHHSSVEGAIMWPTIM 300
           +AHAFSP+DGR H DA E WA G V DSFD+ETVALHEIGHLLGL HSS EGAIMWP + 
Sbjct: 241 VAHAFSPSDGRFHLDADESWANGVVSDSFDLETVALHEIGHLLGLQHSSTEGAIMWPQVP 300

Query: 301 GGATKGLHADDIAGINALY 314
            GA+KGLH DDIAGI ALY
Sbjct: 301 VGASKGLHVDDIAGIKALY 315

[Close]

BLAST of Lsi02G015270 vs. TrEMBL
Match: A0A067DG72_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g035681mg PE=3 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 3.5e-104
Identity = 203/322 (63.04%), Postives = 239/322 (74.22%), Query Frame = 1


Query: 1   MAIKGLSFFISSFFLLIILFPLISSSQPNTAHSH----SHGLVFLNNLNGCKKGDKVEGI 60
           MA K    F     + IIL  L+S +    AHSH    S    F+ +L GC KGDK++GI
Sbjct: 1   MASKAFLSFSIFAVIQIILLSLLSHNYSVLAHSHAEKKSSPFKFIEHLKGCHKGDKIKGI 60

Query: 61  HQMKEYLQHFGYLNH--VQNHSKSFNDDEYDELLESAIKTYQLNYNLKATGALDATTLAL 120
           H++KEY++ FGYLN+   +N S   +DD++DELLESAIKTYQLNY+LK+TG LDA T+A 
Sbjct: 61  HELKEYMEKFGYLNYDNYKNQSTHADDDDFDELLESAIKTYQLNYHLKSTGVLDANTVAK 120

Query: 121 MSKPRCGVADIVDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFEGNPKWPASKSHLTYG 180
           M+ PRCGVADIV+G TRM+SGKK   QH   N HF TVSHY+FF  +PKWPASK  LTY 
Sbjct: 121 MTIPRCGVADIVNGTTRMRSGKKR-QQH---NHHFRTVSHYSFFPRSPKWPASKFSLTYA 180

Query: 181 FLPGTPSEAIAPVGRAFTTWAANTHFSFSQASGYETADIKISFESGDHGDGHAFDGVGG- 240
           F PGT S+AI PV RAF TWAANT F FS+   +  ADIKISFESGDHGDG+ FDG GG 
Sbjct: 181 FRPGTRSDAITPVARAFQTWAANTQFKFSKIEDFANADIKISFESGDHGDGNPFDGRGGP 240

Query: 241 --VIAHAFSPTDGRLHFDAVEPWAVGAVDDSFDVETVALHEIGHLLGLHHSSVEGAIMWP 300
             +IAHAF+PT+GR H+DA EPWAVGAV  +FD+ETVALHEIGHLLGLHHSSVEGAIM+P
Sbjct: 241 EGIIAHAFAPTNGRFHYDADEPWAVGAVQGAFDLETVALHEIGHLLGLHHSSVEGAIMYP 300

Query: 301 TIMGGATKGLHADDIAGINALY 314
           +I  G TKGLH DDI GI  LY
Sbjct: 301 SISAGVTKGLHGDDIQGIRDLY 318

[Close]

BLAST of Lsi02G015270 vs. TrEMBL
Match: B9RUG6_RICCO (Metalloendoproteinase 1, putative OS=Ricinus communis GN=RCOM_0852690 PE=3 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 2.6e-99
Identity = 187/304 (61.51%), Postives = 224/304 (73.68%), Query Frame = 1


Query: 16  LIILFPLISSSQPNTAHSHSH-----GLVFLNNLNGCKKGDKVEGIHQMKEYLQHFGYLN 75
           LI+L  L+SS+    AHSHS         FL +L GC KGD ++GIH +K+YL++FGYL+
Sbjct: 13  LILLISLLSSAA--LAHSHSKTEKSSAFDFLKHLQGCHKGDNLKGIHDLKKYLENFGYLS 72

Query: 76  HV-QNHSKSFNDDEYDELLESAIKTYQLNYNLKATGALDATTLALMSKPRCGVADIVDGK 135
           +  Q+HS   NDD++D+LLE A+KTYQ NY+L  TG LD+ T+  M  PRCGVADI++G 
Sbjct: 73  YKNQSHS---NDDDFDDLLEYALKTYQFNYHLNVTGFLDSETVTKMMMPRCGVADIINGT 132

Query: 136 TRMKSGKKMVNQHRKINGHFHTVSHYAFFEGNPKWPASKSHLTYGFLPGTPSEAIAPVGR 195
           TRM+S  K  N H   +  FHTVSHY FF GNP+WPASK HLTYGFLPGTP++A+ PV +
Sbjct: 133 TRMQSSNK--NPHHHSSTSFHTVSHYEFFPGNPRWPASKYHLTYGFLPGTPNQAMEPVAK 192

Query: 196 AFTTWAANTHFSFSQASGYETADIKISFESGDHGDGHAFDGVGGVIAHAFSPTDGRLHFD 255
           AF TWAANTHF F++   Y  ADI I F  GDHGDG  FDG GG +AHAF+P DGR H+D
Sbjct: 193 AFQTWAANTHFRFTRVQDYRAADITIGFHRGDHGDGSPFDGRGGTLAHAFAPQDGRFHYD 252

Query: 256 AVEPWAVGAVDDSFDVETVALHEIGHLLGLHHSSVEGAIMWPTIMGGATKGLHADDIAGI 314
             E WAVGA   +FDVETVALHEIGHLLGL HSSVEGAIM P+I  GATKGLH+DDI GI
Sbjct: 253 GDEHWAVGATQGAFDVETVALHEIGHLLGLGHSSVEGAIMHPSIQSGATKGLHSDDIQGI 309

[Close]

BLAST of Lsi02G015270 vs. TAIR10
Match: AT1G24140.1 (AT1G24140.1 Matrixin family protein)

HSP 1 Score: 235.0 bits (598), Expect = 6.6e-62
Identity = 127/285 (44.56%), Postives = 169/285 (59.30%), Query Frame = 1


Query: 43  NLNGCKKGDKVEGIHQMKEYLQHFGYLNHVQNHSKSFNDDEYDELLESAIKTYQLNYNLK 102
           N  GC  G K +G++ +K+Y QHFGY+    N S +F DD +D++L++A++ YQ N+ L 
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITET-NLSGNFTDD-FDDILKNAVEMYQRNFQLN 107

Query: 103 ATGALDATTLALMSKPRCGVADIVDGKTRMKSGKKMVNQHRKINGH-FHTVSHYAFFEGN 162
            TG LD  TL  +  PRCG  D+V+G + M SG+K         G  FH V HY+FF G 
Sbjct: 108 VTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGE 167

Query: 163 PKWPASKSHLTYGFLP--GTPSEAIAPVGRAFTTWAANTHFSFSQASGYETADIKISFES 222
           P+WP ++  LTY F P      E  +   RAFT W   T  +F++   + T+DI I F S
Sbjct: 168 PRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYS 227

Query: 223 GDHGDGHAFDGVGGVIAHAFSPTDGRLHFDAVEPWAVG--------AVDDSFDVETVALH 282
           G+HGDG  FDG    +AHAFSP  G  H D  E W V         +V ++ D+E+VA+H
Sbjct: 228 GEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVH 287

Query: 283 EIGHLLGLHHSSVEGAIMWPTIMGGATK-GLHADDIAGINALYTA 316
           EIGHLLGL HSSVEG+IM+PTI  G  K  L  DD+ G+  LY A
Sbjct: 288 EIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYGA 330

[Close]

BLAST of Lsi02G015270 vs. TAIR10
Match: AT1G70170.1 (AT1G70170.1 matrix metalloproteinase)

HSP 1 Score: 231.5 bits (589), Expect = 7.3e-61
Identity = 133/323 (41.18%), Postives = 185/323 (57.28%), Query Frame = 1


Query: 8   FFISSFFLLII-----LFPLISSSQPNTAHSHSHGLVFLNNLNGCKKGDKVEGIHQMKEY 67
           F   S FL++       FP  ++  P+  ++        +N  GC  G  V+G++++K+Y
Sbjct: 6   FGFLSLFLIVSPASAWFFPNSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKKY 65

Query: 68  LQHFGYLNHVQNHSKSFNDDEYDELLESAIKTYQLNYNLKATGALDATTLALMSKPRCGV 127
            Q FGY+   +  S +F DD +D++L++A++ YQ N+NL  TG LDA T+  +  PRCG 
Sbjct: 66  FQRFGYIP--ETFSGNFTDD-FDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGN 125

Query: 128 ADIVDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFEGNPKWPASKSHLTYGFLPGTP-- 187
            D+V+G + M  G++   +      H H V  Y  F G P+WP ++  LTY F P  P  
Sbjct: 126 PDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLT 185

Query: 188 SEAIAPVGRAFTTWAANTHFSFSQASGYETADIKISFESGDHGDGHAFDGVGGVIAHAFS 247
            E  +   RAF  W+  T  +F+ +  + T+DI I F +GDHGDG  FDGV G +AHAFS
Sbjct: 186 EEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFS 245

Query: 248 PTDGRLHFDAVEPWAVGAVDDSF-------DVETVALHEIGHLLGLHHSSVEGAIMWPTI 307
           P  G+ H DA E W V    DSF       D+E+VA+HEIGHLLGL HSSVE +IM+PTI
Sbjct: 246 PPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTI 305

Query: 308 MGGATK-GLHADDIAGINALYTA 316
             G  K  L  DD+ GI  LY A
Sbjct: 306 TTGKRKVDLTNDDVEGIQYLYGA 325

[Close]

BLAST of Lsi02G015270 vs. TAIR10
Match: AT1G59970.1 (AT1G59970.1 Matrixin family protein)

HSP 1 Score: 221.5 bits (563), Expect = 7.5e-58
Identity = 125/284 (44.01%), Postives = 161/284 (56.69%), Query Frame = 1


Query: 42  NNLNGCKKGDKVEGIHQMKEYLQHFGYLNHVQNHSKSFNDDEYDELLESAIKTYQLNYNL 101
           + L GC  G+ + G+ ++K+Y + FGY+    N +     D++D++L+SAI TYQ N+NL
Sbjct: 46  SKLAGCHIGENINGLSKLKQYFRRFGYITTTGNCT-----DDFDDVLQSAINTYQKNFNL 105

Query: 102 KATGALDATTLALMSKPRCGVADIVDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFEGN 161
           K TG LD++TL  + KPRCG  D++DG + M  GK +            T   Y+FF G 
Sbjct: 106 KVTGKLDSSTLRQIVKPRCGNPDLIDGVSEMNGGKIL-----------RTTEKYSFFPGK 165

Query: 162 PKWPASKSHLTYGFLP--GTPSEAIAPVGRAFTTWAANTHFSFSQASGYETADIKISFES 221
           P+WP  K  LTY F P      E      RAFT WA  T  +F+++     ADI I F S
Sbjct: 166 PRWPKRKRDLTYAFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFS 225

Query: 222 GDHGDGHAFDGVGGVIAHAFSPTDGRLHFDAVEPWAVG---------AVDDSFDVETVAL 281
           G+HGDG  FDG  G +AHA SP  G LH D  E W +           V    D+E+VA+
Sbjct: 226 GEHGDGEPFDGAMGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAV 285

Query: 282 HEIGHLLGLHHSSVEGAIMWPTIMGGATK-GLHADDIAGINALY 314
           HEIGHLLGL HSSVE AIM+P I GG  K  L  DDI GI  LY
Sbjct: 286 HEIGHLLGLGHSSVEDAIMFPAISGGDRKVELAKDDIEGIQHLY 313

[Close]

BLAST of Lsi02G015270 vs. TAIR10
Match: AT4G16640.1 (AT4G16640.1 Matrixin family protein)

HSP 1 Score: 201.4 bits (511), Expect = 8.0e-52
Identity = 123/277 (44.40%), Postives = 160/277 (57.76%), Query Frame = 1


Query: 50  GDKVEGIHQMKEYLQHFGYLNHVQNHSKSFNDDEYDELLESAIKTYQLNYNLKATGALDA 109
           G  V G+ ++K YL  FGY+N   + S+ F+D  +D  LESAI  YQ N  L  TG LD 
Sbjct: 64  GSHVSGVSELKRYLHRFGYVN---DGSEIFSD-VFDGPLESAISLYQENLGLPITGRLDT 123

Query: 110 TTLALMSKPRCGVADIVDGKTRMKSGKKMVNQHRKINGHF-HTVSHYAFFEGNPKWPASK 169
           +T+ LMS PRCGV+D                 H  IN  F HT +HY +F G PKW  ++
Sbjct: 124 STVTLMSLPRCGVSDT----------------HMTINNDFLHTTAHYTYFNGKPKW--NR 183

Query: 170 SHLTYGF-----LPGTPSEAIAPV-GRAFTTWAANTHFSFSQASGYETADIKISFESGDH 229
             LTY       L    SE +  V  RAF+ W++    SF +   + TAD+KI F +GDH
Sbjct: 184 DTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDH 243

Query: 230 GDGHAFDGVGGVIAHAFSPTDGRLHFDAVEPWAV-----GAVDDSFDVETVALHEIGHLL 289
           GDG  FDGV G +AHAF+P +GRLH DA E W V     G+ + + D+E+VA HEIGHLL
Sbjct: 244 GDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLL 303

Query: 290 GLHHSSVEGAIMWPTIMGGATK-GLHADDIAGINALY 314
           GL HSS E A+M+P++     K  L  DD+AG+  LY
Sbjct: 304 GLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]

BLAST of Lsi02G015270 vs. TAIR10
Match: AT2G45040.1 (AT2G45040.1 Matrixin family protein)

HSP 1 Score: 174.9 bits (442), Expect = 8.1e-44
Identity = 109/270 (40.37%), Postives = 143/270 (52.96%), Query Frame = 1


Query: 56  IHQMKEYLQHFGYLNHVQNHSKSFNDDEYDELLESAIKTYQLNYNLKATGALDATTLALM 115
           I ++K +LQ +GYL          N +  D   E A+  YQ N  L  TG  D+ TL+ +
Sbjct: 50  IPEIKRHLQQYGYLPQ--------NKESDDVSFEQALVRYQKNLGLPITGKPDSDTLSQI 109

Query: 116 SKPRCGVADIVDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFEGNPKWPASKS-HLTYG 175
             PRCG  D V+ KT                  FHT   Y +F G P+W       LTY 
Sbjct: 110 LLPRCGFPDDVEPKT----------------APFHTGKKYVYFPGRPRWTRDVPLKLTYA 169

Query: 176 FLPGTPSEAIAPVG------RAFTTWAANTHFSFSQASGYETADIKISFESGDHGDGHAF 235
           F     +  +AP        RAF  WA+    SF +   Y  ADIKI F +GDHGDG  F
Sbjct: 170 FSQENLTPYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPF 229

Query: 236 DGVGGVIAHAFSPTDGRLHFDAVEPWAVGAVDD----SFDVETVALHEIGHLLGLHHSSV 295
           DGV GV+AH FSP +GRLH D  E WAV   ++    + D+E+VA+HEIGH+LGL HSSV
Sbjct: 230 DGVLGVLAHTFSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSV 289

Query: 296 EGAIMWPTIMGGATK-GLHADDIAGINALY 314
           + A M+PT+   + K  L+ DD+ G+ +LY
Sbjct: 290 KDAAMYPTLKPRSKKVNLNMDDVVGVQSLY 295

[Close]

BLAST of Lsi02G015270 vs. NCBI nr
Match: gi|659118019|ref|XP_008458907.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 528.1 bits (1359), Expect = 1.1e-146
Identity = 258/311 (82.96%), Postives = 283/311 (91.00%), Query Frame = 1


Query: 6   LSFFISSFFLLIILFPLISSSQPNTAHSHSHGLVFLNNLNGCKKGDKVEGIHQMKEYLQH 65
           LSF  +SFFL+I+LFPLISSS PN    HS+GL FL NLNGC+KGDKV GIHQ+KEYLQH
Sbjct: 7   LSFLTTSFFLVIVLFPLISSSHPNI--HHSNGLAFLKNLNGCRKGDKVAGIHQIKEYLQH 66

Query: 66  FGYLN-HVQNHSKSFNDDEYDELLESAIKTYQLNYNLKATGALDATTLALMSKPRCGVAD 125
           FGYLN ++QN+SKSF+DDE+D +LESAIKTYQLNYNLKATG LDA TL LMSKPRCGVAD
Sbjct: 67  FGYLNNYLQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTLDAKTLDLMSKPRCGVAD 126

Query: 126 IVDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFEGNPKWPASKSHLTYGFLPGTPSEAI 185
           I+DGKTRMKSGKKMVNQHRKINGHFHTVSHYAFF+GNPKWPASK HLTYGFLPGTPS+A+
Sbjct: 127 IIDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFDGNPKWPASKFHLTYGFLPGTPSKAV 186

Query: 186 APVGRAFTTWAANTHFSFSQASGYETADIKISFESGDHGDGHAFDGVGGVIAHAFSPTDG 245
           APVGRAF TW A+THF+FS+AS Y+ ADIKISF SGDHGDGH+FDGVGGVIAH F+PTDG
Sbjct: 187 APVGRAFKTWGAHTHFNFSRASKYKKADIKISFRSGDHGDGHSFDGVGGVIAHGFAPTDG 246

Query: 246 RLHFDAVEPWAVGAVDDSFDVETVALHEIGHLLGLHHSSVEGAIMWPTIMGGATKGLHAD 305
           RLHFDA+E WAVGAV DSFD+ETVALHEIGHLLGLHHSSVEGAIMWP+IM G TKGL AD
Sbjct: 247 RLHFDAIESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGTTKGLDAD 306

Query: 306 DIAGINALYTA 316
           DIAGINALY A
Sbjct: 307 DIAGINALYNA 315

[Close]

BLAST of Lsi02G015270 vs. NCBI nr
Match: gi|449449417|ref|XP_004142461.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 511.1 bits (1315), Expect = 1.3e-141
Identity = 251/312 (80.45%), Postives = 281/312 (90.06%), Query Frame = 1


Query: 6   LSFFISSFFLLIILFPLISSSQPNTAHSH-SHGLVFLNNLNGCKKGDKVEGIHQMKEYLQ 65
           LSF  +SFFL+I+LFPLISSS P    SH S+GLVFL NLNGCKKGDKVEGIHQ+KEYLQ
Sbjct: 7   LSFLTTSFFLVIVLFPLISSSHPK---SHLSNGLVFLKNLNGCKKGDKVEGIHQVKEYLQ 66

Query: 66  HFGYLN-HVQNHSKSFNDDEYDELLESAIKTYQLNYNLKATGALDATTLALMSKPRCGVA 125
           HFGYLN +VQN+SKSF+DDE+D +LESAIKTYQLNYNLKATG ++A TL LMS+PRCG A
Sbjct: 67  HFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCGFA 126

Query: 126 DIVDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFEGNPKWPASKSHLTYGFLPGTPSEA 185
           DIVDGKTRMKSGKKMVNQHRKINGHFH+VSHYAFF+GNPKWPASKSHLTYGFLPGTPS+A
Sbjct: 127 DIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTPSKA 186

Query: 186 IAPVGRAFTTWAANTHFSFSQASGYETADIKISFESGDHGDGHAFDGVGGVIAHAFSPTD 245
           +A +GRAF TW ANTHF+FSQ S Y+ ADIKISFE+GDHGDGH+FDGV GVIAH F+P D
Sbjct: 187 VATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFAPRD 246

Query: 246 GRLHFDAVEPWAVGAVDDSFDVETVALHEIGHLLGLHHSSVEGAIMWPTIMGGATKGLHA 305
           GRLHFDA E WAVGA+ DSFD+ET+ALHEIGHLLGL+HSS+E AIMWP+IM G+TKGL A
Sbjct: 247 GRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKGLDA 306

Query: 306 DDIAGINALYTA 316
           DDIAGINALY A
Sbjct: 307 DDIAGINALYNA 315

[Close]

BLAST of Lsi02G015270 vs. NCBI nr
Match: gi|449449409|ref|XP_004142457.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 485.0 bits (1247), Expect = 1.0e-133
Identity = 241/317 (76.03%), Postives = 266/317 (83.91%), Query Frame = 1


Query: 1   MAIKGLS-FFISSFFLLIILFPLISSSQPNTAHSHSHGLVFLNNLNGCKKGDKVEGIHQM 60
           M  +GLS FF  + FL+I LFPLISSSQP        GLVFL NL GCKKGDKVEGIHQM
Sbjct: 1   MGFQGLSLFFTFTLFLIISLFPLISSSQPKPVD----GLVFLKNLKGCKKGDKVEGIHQM 60

Query: 61  KEYLQHFGYLNHVQNHSKSFNDDEYDELLESAIKTYQLNYNLKATGALDATTLALMSKPR 120
           K+YLQHFGYLN+  +     +DDE+DE+LE A+KTYQ+NYNLK TG LD +TL  MSKPR
Sbjct: 61  KKYLQHFGYLNNDVHIRSESSDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKPR 120

Query: 121 CGVADIVDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFEGNPKWPASKSHLTYGFLPGT 180
           CGVADI++GKT MKSGK+ VNQHRKI+GHFH VSH+AFFEGN +WP SKSHLTYGFLPGT
Sbjct: 121 CGVADIINGKTSMKSGKRFVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGFLPGT 180

Query: 181 PSEAIAPVGRAFTTWAANTHFSFSQASGYETADIKISFESGDHGDGHAFDGVGGVIAHAF 240
           PSEAI+PV RAFTTWAANTHFSFSQ S YE ADIKISFE GDHGDG  FD VGGV+AHAF
Sbjct: 181 PSEAISPVNRAFTTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVLAHAF 240

Query: 241 SPTDGRLHFDAVEPWAVGAVDDSFDVETVALHEIGHLLGLHHSSVEGAIMWPTIMGGATK 300
           +PTDGRLHFDAVE WA GAV  S+D+ETVALHEIGHLLGLHHSSVEGAIMWP+IMGGATK
Sbjct: 241 APTDGRLHFDAVEHWADGAVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIMGGATK 300

Query: 301 GLHADDIAGINALYTAT 317
           GLHADDI GI  LYT +
Sbjct: 301 GLHADDIEGIKVLYTTS 313

[Close]

BLAST of Lsi02G015270 vs. NCBI nr
Match: gi|659117646|ref|XP_008458713.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 465.3 bits (1196), Expect = 8.5e-128
Identity = 223/281 (79.36%), Postives = 251/281 (89.32%), Query Frame = 1


Query: 37  GLVFLNNLNGCKKGDKVEGIHQMKEYLQHFGYLNH-VQNHSKSFNDDEYDELLESAIKTY 96
           GLVFL NLNGCKKGDKVEGIHQMK+YLQHFGY+N+ V  HSKS +DDE+DE+LE A+KTY
Sbjct: 3   GLVFLKNLNGCKKGDKVEGIHQMKKYLQHFGYMNNDVHIHSKS-SDDEFDEILEFAVKTY 62

Query: 97  QLNYNLKATGALDATTLALMSKPRCGVADIVDGKTRMKSGKKMVNQHRKINGHFHTVSHY 156
           Q+NYNLKATG LD +T++ MSKPRCGVADI++G+T MKSGK++VN+HRKI GHFH VSH+
Sbjct: 63  QINYNLKATGTLDNSTISQMSKPRCGVADIINGRTGMKSGKRLVNRHRKIIGHFHEVSHF 122

Query: 157 AFFEGNPKWPASKSHLTYGFLPGTPSEAIAPVGRAFTTWAANTHFSFSQASGYETADIKI 216
           AFFEGN KWP SKSHLTYGFLPGTPSEAI+PV RAFTTWAANTHFSFSQ S YE ADIK+
Sbjct: 123 AFFEGNLKWPNSKSHLTYGFLPGTPSEAISPVNRAFTTWAANTHFSFSQESNYENADIKV 182

Query: 217 SFESGDHGDGHAFDGVGGVIAHAFSPTDGRLHFDAVEPWAVGAVDDSFDVETVALHEIGH 276
           +FE GDHGDG  FD VGGV+AHAF+PTDGRLHFDAVE WA GAV +S+D+ETVALHEIGH
Sbjct: 183 TFERGDHGDGFPFDSVGGVLAHAFAPTDGRLHFDAVEHWADGAVPNSYDMETVALHEIGH 242

Query: 277 LLGLHHSSVEGAIMWPTIMGGATKGLHADDIAGINALYTAT 317
           LLGLHHSSVEGAIMWP+IMGG TKGLHADDI GI  LYT +
Sbjct: 243 LLGLHHSSVEGAIMWPSIMGGTTKGLHADDIEGIKVLYTTS 282

[Close]

BLAST of Lsi02G015270 vs. NCBI nr
Match: gi|449449419|ref|XP_004142462.1| (PREDICTED: metalloendoproteinase 1 [Cucumis sativus])

HSP 1 Score: 435.3 bits (1118), Expect = 9.4e-119
Identity = 222/319 (69.59%), Postives = 253/319 (79.31%), Query Frame = 1


Query: 1   MAIKGLSFFISSFFLLIILFPLISSSQPNTAHSHSHG-----LVFLNNLNGCKKGDKVEG 60
           MA++ LSFF  +  LL+  FPLISSSQ N  HSHSHG      VFLN L G +KGDKVEG
Sbjct: 1   MALEELSFFTFTL-LLLTFFPLISSSQSN--HSHSHGKASSQFVFLNQLYGSQKGDKVEG 60

Query: 61  IHQMKEYLQHFGYLNH-VQNHSKSFNDDEYDELLESAIKTYQLNYNLKATGALDATTLAL 120
           IHQ+K+YL  FGYLN   Q HS++ NDDE+DELLE+AIKTYQ NYNLK TG LDA T+A 
Sbjct: 61  IHQLKKYLHQFGYLNEDAQIHSEN-NDDEFDELLETAIKTYQKNYNLKVTGTLDAMTIAQ 120

Query: 121 MSKPRCGVADIVDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFEGNPKWPASKSHLTYG 180
           MSKPRCGVADI+ G T M+S KK       I GHFH+VSH+AFF+GNPKWPA+KSHLTYG
Sbjct: 121 MSKPRCGVADIIHGNTWMRSSKKRKQLEHGIVGHFHSVSHFAFFDGNPKWPATKSHLTYG 180

Query: 181 FLPGTPSEAIAPVGRAFTTWAANTHFSFSQASGYETADIKISFESGDHGDGHAFDGVGGV 240
           FLPGTPSE ++PV RAF TWAAN+HF+FSQA   +T+DI I FE+GDHGDG+ FDGVGGV
Sbjct: 181 FLPGTPSETVSPVARAFATWAANSHFTFSQALDNQTSDITIGFETGDHGDGYPFDGVGGV 240

Query: 241 IAHAFSPTDGRLHFDAVEPWAVGAVDDSFDVETVALHEIGHLLGLHHSSVEGAIMWPTIM 300
           +AHAFSP+DGR H DA E WA G V DSFD+ETVALHEIGHLLGL HSS EGAIMWP + 
Sbjct: 241 VAHAFSPSDGRFHLDADESWANGVVSDSFDLETVALHEIGHLLGLQHSSTEGAIMWPQVP 300

Query: 301 GGATKGLHADDIAGINALY 314
            GA+KGLH DDIAGI ALY
Sbjct: 301 VGASKGLHVDDIAGIKALY 315

The following BLAST results are available for this feature:

BLAST of Lsi02G015270 vs. Swiss-Prot


Match Name	E-value	Identity	Description
3MMP_ARATH	1.2e-60	44.56	Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1	[more]
2MMP_ARATH	1.3e-59	41.18	Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1	[more]
5MMP_ARATH	1.3e-56	44.01	Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1	[more]
MEP1_SOYBN	2.2e-51	42.49	Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2	[more]
1MMP_ARATH	1.4e-50	44.40	Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1	[more]

BLAST of Lsi02G015270 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KU08_CUCSA	9.4e-142	80.45	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632130 PE=3 SV=1	[more]
A0A0A0KS47_CUCSA	7.2e-134	76.03	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G633160 PE=3 SV=1	[more]
A0A0A0KS39_CUCSA	6.5e-119	69.59	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632120 PE=3 SV=1	[more]
A0A067DG72_CITSI	3.5e-104	63.04	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g035681mg PE=3 SV=1	[more]
B9RUG6_RICCO	2.6e-99	61.51	Metalloendoproteinase 1, putative OS=Ricinus communis GN=RCOM_0852690 PE=3 SV=1	[more]

BLAST of Lsi02G015270 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G24140.1	6.6e-62	44.56	Matrixin family protein	[more]
AT1G70170.1	7.3e-61	41.18	matrix metalloproteinase	[more]
AT1G59970.1	7.5e-58	44.01	Matrixin family protein	[more]
AT4G16640.1	8.0e-52	44.40	Matrixin family protein	[more]
AT2G45040.1	8.1e-44	40.37	Matrixin family protein	[more]

BLAST of Lsi02G015270 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659118019\|ref\|XP_008458907.1\|	1.1e-146	82.96	PREDICTED: metalloendoproteinase 1-like [Cucumis melo]	[more]
gi\|449449417\|ref\|XP_004142461.1\|	1.3e-141	80.45	PREDICTED: metalloendoproteinase 1-like [Cucumis sativus]	[more]
gi\|449449409\|ref\|XP_004142457.1\|	1.0e-133	76.03	PREDICTED: metalloendoproteinase 1-like [Cucumis sativus]	[more]
gi\|659117646\|ref\|XP_008458713.1\|	8.5e-128	79.36	PREDICTED: metalloendoproteinase 1-like [Cucumis melo]	[more]
gi\|449449419\|ref\|XP_004142462.1\|	9.4e-119	69.59	PREDICTED: metalloendoproteinase 1 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding
GO:0008237	metallopeptidase activity
GO:0008270	zinc ion binding
GO:0004222	metalloendopeptidase activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0031012	extracellular matrix

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

Vocabulary: **INTERPRO**
Term	Definition
IPR024079	MetalloPept_cat_dom_sf
IPR021190	Pept_M10A
IPR016293	Peptidase M10A, stromelysin-type
IPR006026	Peptidase_Metallo
IPR002477	Peptidoglycan-bd-like
IPR001818	Pept_M10_metallopeptidase

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0031012	extracellular matrix
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0008237	metallopeptidase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G015270.1	Lsi02G015270.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 164..313 score: 3.7
IPR002477	Peptidoglycan binding-like	GENE3D	G3DSA:1.10.101.10		coord: 55..116 score: 1.8
IPR002477	Peptidoglycan binding-like	PFAM	PF01471	PG_binding_1	coord: 55..115 score: 9.6
IPR002477	Peptidoglycan binding-like	unknown	SSF47090	PGBD-like	coord: 47..125 score: 2.67
IPR006026	Peptidase, metallopeptidase	SMART	SM00235	col_5	coord: 160..315 score: 2.9
IPR016293	Peptidase M10A, stromelysin-type	PIR	PIRSF001191	MMP_stromelysin	coord: 21..317 score: 1.6
IPR021190	Peptidase M10A	PRINTS	PR00138	MATRIXIN	coord: 187..202 score: 1.3E-34coord: 301..314 score: 1.3E-34coord: 115..128 score: 1.3E-34coord: 268..293 score: 1.3E-34coord: 211..239 score: 1.3
IPR024079	Metallopeptidase, catalytic domain	GENE3D	G3DSA:3.40.390.10		coord: 117..315 score: 4.0
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 3..24 score: 5.4E-122coord: 41..128 score: 5.4E-122coord: 150..313 score: 5.4E
None	No IPR available	PANTHER	PTHR10201:SF140	F20P5.11 PROTEIN	coord: 41..128 score: 5.4E-122coord: 150..313 score: 5.4E-122coord: 3..24 score: 5.4E
None	No IPR available	unknown	SSF55486	Metalloproteases ("zincins"), catalytic domain	coord: 157..314 score: 1.71

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi02G015270	Cucsa.158720	Cucumber (Gy14) v1	cgylsiB251
Lsi02G015270	CmoCh04G028590	Cucurbita moschata (Rifu)	cmolsiB640

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi02G015270	Watermelon (97103) v2	lsiwmbB161
Lsi02G015270	Watermelon (97103) v2	lsiwmbB208
Lsi02G015270	Wax gourd	lsiwgoB207
Lsi02G015270	Wax gourd	lsiwgoB253
Lsi02G015270	Bottle gourd (USVL1VR-Ls)	lsilsiB089
Lsi02G015270	Cucumber (Gy14) v1	cgylsiB118
Lsi02G015270	Cucurbita maxima (Rimu)	cmalsiB272
Lsi02G015270	Cucurbita maxima (Rimu)	cmalsiB372
Lsi02G015270	Cucurbita maxima (Rimu)	cmalsiB651
Lsi02G015270	Cucurbita moschata (Rifu)	cmolsiB261
Lsi02G015270	Cucurbita pepo (Zucchini)	cpelsiB141
Lsi02G015270	Cucurbita pepo (Zucchini)	cpelsiB336
Lsi02G015270	Wild cucumber (PI 183967)	cpilsiB028
Lsi02G015270	Wild cucumber (PI 183967)	cpilsiB352
Lsi02G015270	Cucumber (Chinese Long) v2	culsiB028
Lsi02G015270	Cucumber (Chinese Long) v2	culsiB348
Lsi02G015270	Melon (DHL92) v3.5.1	lsimeB162
Lsi02G015270	Melon (DHL92) v3.5.1	lsimeB182
Lsi02G015270	Watermelon (Charleston Gray)	lsiwcgB155
Lsi02G015270	Watermelon (Charleston Gray)	lsiwcgB176
Lsi02G015270	Watermelon (97103) v1	lsiwmB170
Lsi02G015270	Cucumber (Gy14) v2	cgyblsiB024
Lsi02G015270	Cucumber (Gy14) v2	cgyblsiB319
Lsi02G015270	Melon (DHL92) v3.6.1	lsimedB191
Lsi02G015270	Melon (DHL92) v3.6.1	lsimedB171
Lsi02G015270	Silver-seed gourd	carlsiB232
Lsi02G015270	Silver-seed gourd	carlsiB623
Lsi02G015270	Cucumber (Chinese Long) v3	cuclsiB028
Lsi02G015270	Cucumber (Chinese Long) v3	cuclsiB371