Lsi02G009770 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi02G009770
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionFAD-binding Berberine family protein
Locationchr02 : 9821788 .. 9826543 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTATTCTTCTATCTTCGTCTTCACCATGGGTTGCATCTAATACTTTTGAAAGACATGAAAATTTTCTTCAATGTCTTTCCCATCATTCTCCAAACATTTCCAAAGTCATTTACACTCCCTTCAACTCCTCTTATTCTTCCATCTTAAACTTCACCCTTAGAAACCTAAGATTCTCAACCCCCAATACCCCAAAGCCACTCCTCATCATCACTCCCTCACATATTTCCCACATCCAAGCCGCCGTCATCTGTTCCAAACCCTACGGTCTTCAAATCCGAACTCGAAGTGGTGGCCATGATTTCGAGGGCCTTTCTTACGTCGCCTACCTTCCATTCGTCATCCTTGACCTAATAAATTTAAGCTCTATCTCCATCGATGTCGAAACTAACACTGCATGGGTTGAGTCCGGTGCAACCATAGGTGAACTTTACTATAGAATCGCCGAGAAAAGTCGAACCTTGGCTTTTCCGGCCGGTACTTGTCCGATAGTCGGCGTTGGCGGGCAGTTCAGTGGCGGCGGATATGGGATGTTGACGAGGAAATATGGTCTTGCTTCTGATAATGTGATAGATGCTTATTTGGTTGATGCAAATGGGGAGTTTCATGATAGAAAGTCAATGGGGGAGGATTTGTTTTGGGCCATTAGAGGCGGCGGGGGAGGGAGCTTTGGAATAGTGGTGGCGTGGAAGGTGAAGCTGGTTCCGGTGCCGGCGACGGTGACGCTTTGCTTTGTTAACAAAAATTCGGAGGAAGGAGCAACCAATCTAATTCATCGGTGGCAATATGTTGCTCACGACTTAGATGAAAATATATTCCTTGGGATTCTTTTGGCTGGTATTTTCTTCAACGCTTTGCCTTGTGTTATTTGTTAATTATTTCTTCCCTTTGCATTCAAAGCCTTTTTTTTCTTAGATTAATATTGAAATGAAAAGGATAGCATACCGGCCAACAGATGGTTTTAACAATACTATAGATGTATATTAAAATGAGAGATTATTCCTCTCTTTTCTGACGTTTAAAAAGTTCTAATTTTATCAAAATATTTTAAATAAAAAATATTGATCGTGTTTAATAATCATTTAATTTCTTTTTTACTTTTTATTTCATTTTTTCTATTATTAGTTTTCTGTTTTTCAAAAAATAAAAACCATAAAATGACTTTTTTTTTTTTTTTAGAGAAAATTGTGTAAAGATACGTAAATAAAAATTTAGAAACATAGATACAAATGTTTTAGAGAGAAAAGTAAAAAACATGTAAATTTAGAGGCGTGAAAGAAAAATATTTATTTAGATGAAATAAATTAAATAGAGATGTATTTGAGTGAAAATATTAGGTAAACTCCATAAACACATCAGTTAGAAAAAAAAATAAACAAAAGTTTGAGGAAAAGAGATAAACTTTAGAGAAAATAAAGCCAAATAAAAAGATTAAATTTTAAAGAGAGAAAATTTATGAGTAAATGATATGTTAAATAAATAAGTATAAATGAGAAAATAAATAAATAAAAATTGTAGTAAGAGTATTATTAAGAGAGAAGAAAACTCGAAAGAAAAAAAGTTAATTAGAGATTTATTTATTAGAGAGAGAAAAAGTTGGAAATAAATAGAATAAATAAAAGAGAAAGAGAAAGAGAGAGAGAAATGAAAGTTAAATAAACATCAACGTTTTAGAGATAAAAAAAAATTTAAAAACATGAAACGGGACCATAATAAGTGTTGACAAACATGCACATTTTAAATAATGTGAGAAATTTAAATTTACCAAAAAAAACTTATAAAAAACAAGACTCCATTTTTAAAACAGTGAAATAGATTATCAAACAAGACCATAATAAGTGTTGATATTATTATTTTAACTTTTTTTCGTACAATAAGAATAGAAAGATTCAAACCATAAATTTCTTTGTCATGCTAATATATTTGTATGGTAGTTGAGCTATGCTCACTTTAGTGAGAAGTAACTCATGTATCTTATAAATATTGTGAGGTTTCTTCTCAACATGTCTCCTCTAGATTGGTGCCTCTTTAGGTTCACCATTCTTAGATTGAGTCCCAATTTTTATTTTTGGATTGAATGTCTAATTTGTGTTAATTGGTGATTTAACATGATATAAGAGCCAAGAGGTAACATGGTAGTGAGAACATAAAAAAGGTCAAAGAGCTAAGGGGTCGTGGGTTCAATCCATGGTGTCCACCTACTTAGGATGATTCCCATTTCATTTTCTTCCTACCCATTTTATTTCAACCGCTCACATTCCCTCCCCCTTCTCTTTTCCCTTCTCTCTTACATCCACACTTTGTCTCTCTCTTCCCATCTCTTTCACATTTTCTCTCGGTCACTCTCTCTTACACAAAAGAGGGAGACCCCCTCTCTCACATCCCTCACACAAACAAGAAAGAAGAAATAATTTAGAAGAGGAAGAGAAGAAGTTTTCAAAGAATTTGGTTTTTGCTTTTGATTTTATGCTTCTCCGTCTTTTTCATTTTGAATATGATGTGTTCACAAAAAACGAAGATTTACCTGACTTGACTCGCTTCAGACTCGAATTAAGTTAGATCGGGTGATTCGGGTCTTGTTCGTACAAAATTGAATTAGGCGGATCGAGTCATTTTTAAAAGAAAAAATCGACCCAACCCAACCCATTTACACCCCTATGAAGAAATACAATGATCAAATCTCTATAATGTTATTTCCTCTCCGATTCATATTGAATTTCACTTGTTGGTTGTTTAACAAATTTTCAAGCCACACAAGTGAGGGAGAGTGTTAGAGTGTGTTGATATAGTTAAATTTATCGTAACCCATTAATTAACTTAACCTTTTAGGTCAATTCATGATTTAAGTTTATTATAACCCATGTGAAATCACCAGTTGATCTTAAAACTTGAGTTGATGAGTTATGGTAACTGTAATTATATCAATATATACTTCAATATCGATTTCATAAACTTGAGATCAATTAAAAAATCAAATTTAAACCTTGTTGACCAATAATCATATATATAACCTCGGAACCTCAAACAGAAAATAGAAAACAAGTTCAAATCTAAATTATCAAAATGGTTAGTTTTGAAAACTTTAGAGACAGGGACCGAAGTGTGTTTTACTCAAATTTCTATTTTAATTCTTAAACCGTCCACCATTTGACATTTCTATTCCCAAATGAAAGAAAAAACACTTTAAACATTTAACACCAAATCTCATGACAAAAAATGAACTTTAAAGCAATAAAAAGAAAAAAAAAATACTCAATTCATCCATTAAAGAGGTAATTAAGAAATTGAGGTCTTTCTTTAAAAAAAATTTCATCCTAACTTTTTCTTTTAGTATAATAAGATGTTAGGTATTCAGACTACAAACTTTCTTAATTACTATTGCTAACATATACTATATGTTAGTTGAGCTACATTCGCTTTTGACAATTTTCTCCCTAACCTGGCTATATTAAAATCACAAAAGCATGAATTATTAGATATATATCTAATCTTGATGATGATAGGTGGGAATTTTACTAGGCAAGAAGGCAAAACAAACCCAACAAATTTTTATTCTTCTCTTTGTATCTTAGGAGGTCCTATATTCAAATACTTGTAATGTCATTTCCTCCCTACCTATTATTGCATTCACTTGTTGGCTCTTTAACAAATTTTCAAGCCACAAAGGGGAGGAGGGAGGAGGGGAGGAGGGGATGCTAGAGTGTGTTGATATAGTTAAATTTATTATAACCAATCAGCTTAAACTTTTGGATCAATCGGCGATTTAAGTTTATCATAACTCATACGCTAAATTACCAATTGACCCAAAAATAATTTAATTCTTCTAGTACAATAGGGATTGGGAGTTCAAACCACATACCCTCTTGATTGTTATTACTAACACTTACAATATGCTAATTGAGCTATACTCCCTTTTGGCAATCTCATCCCTACCATATTAAAACCACTATGAATGTAAAAGTATGAATTATTAGATATATAACTCATGTTTTGATGATGATAGGTGGGAATTTTACATGGCAAGAAGGCAAAATAAATCCAACAGCTTTATTCCTCTCTTTGTATCTTGGGAAGGTAGAAGAGCTTGTGTCAATCCTCAACACAACTTTTCCTGAATTGGGTTTGGCAAAGAAAGATTGCACAGAAACAAGCTGGGTTGAATCAACCCTCATCATGGGCACTGGCCTCCAACCAGGGAAGGCATTAGAAGATCTACTAAGTAAAGCATCTCTAACCAATGCAAGTACAAAAATCAAATCTGATTACATTAAACAACCCATCCCTATGGCCGCAGTTGAGGGCATATGGCAAAGATTAAAACTTGAAGATATAGAAATGCCACAACTTCTACTTTCTCCTTATGGTGGAAGAATGAGCCAGATTTCTGAGTCAGAAACTCCTTTTTCACATAGGTCGGAATATTTATACACAATTGGTTACGTCATTAATTGGGAACAACAAGGTAATGATGTTGAGAAAAGACACCTAAGTTGGATACGAGAGCTTTATAGTTACATGGCTCCTTTTGCTTCAAAATTACCTAGAGCTGCATATGTCAACTATAGAGACCTTGATATTGGATCAAACTATAGATATGGAAAGACAAGCTACGAGAAAGCTAGCATTTGGGGTTTGAAATATTTTGGAAATAATTTTAATAGATTGGTGGATGTTAAGACTAGGTTTGATCCTTATGATTTCTTTAGGCATGAACAAAGCATACCTACCCTCTCAAACGAAAAGGTATTTTACTGA

mRNA sequence

ATGTCTATTCTTCTATCTTCGTCTTCACCATGGGTTGCATCTAATACTTTTGAAAGACATGAAAATTTTCTTCAATGTCTTTCCCATCATTCTCCAAACATTTCCAAAGTCATTTACACTCCCTTCAACTCCTCTTATTCTTCCATCTTAAACTTCACCCTTAGAAACCTAAGATTCTCAACCCCCAATACCCCAAAGCCACTCCTCATCATCACTCCCTCACATATTTCCCACATCCAAGCCGCCGTCATCTGTTCCAAACCCTACGGTCTTCAAATCCGAACTCGAAGTGGTGGCCATGATTTCGAGGGCCTTTCTTACGTCGCCTACCTTCCATTCGTCATCCTTGACCTAATAAATTTAAGCTCTATCTCCATCGATGTCGAAACTAACACTGCATGGGTTGAGTCCGGTGCAACCATAGGTGAACTTTACTATAGAATCGCCGAGAAAAGTCGAACCTTGGCTTTTCCGGCCGGTACTTGTCCGATAGTCGGCGTTGGCGGGCAGTTCAGTGGCGGCGGATATGGGATGTTGACGAGGAAATATGGTCTTGCTTCTGATAATGTGATAGATGCTTATTTGGTTGATGCAAATGGGGAGTTTCATGATAGAAAGTCAATGGGGGAGGATTTGTTTTGGGCCATTAGAGGCGGCGGGGGAGGGAGCTTTGGAATAGTGGTGGCGTGGAAGGTGAAGCTGGTTCCGGTGCCGGCGACGGTGACGCTTTGCTTTGTTAACAAAAATTCGGAGGAAGGAGCAACCAATCTAATTCATCGGTGGCAATATGTTGCTCACGACTTAGATGAAAATATATTCCTTGGGATTCTTTTGGCTGGTGGGAATTTTACATGGCAAGAAGGCAAAATAAATCCAACAGCTTTATTCCTCTCTTTGTATCTTGGGAAGGTAGAAGAGCTTGTGTCAATCCTCAACACAACTTTTCCTGAATTGGGTTTGGCAAAGAAAGATTGCACAGAAACAAGCTGGGTTGAATCAACCCTCATCATGGGCACTGGCCTCCAACCAGGGAAGGCATTAGAAGATCTACTAAGTAAAGCATCTCTAACCAATGCAAGTACAAAAATCAAATCTGATTACATTAAACAACCCATCCCTATGGCCGCAGTTGAGGGCATATGGCAAAGATTAAAACTTGAAGATATAGAAATGCCACAACTTCTACTTTCTCCTTATGGTGGAAGAATGAGCCAGATTTCTGAGTCAGAAACTCCTTTTTCACATAGGTCGGAATATTTATACACAATTGGTTACGTCATTAATTGGGAACAACAAGGTAATGATGTTGAGAAAAGACACCTAAGTTGGATACGAGAGCTTTATAGTTACATGGCTCCTTTTGCTTCAAAATTACCTAGAGCTGCATATGTCAACTATAGAGACCTTGATATTGGATCAAACTATAGATATGGAAAGACAAGCTACGAGAAAGCTAGCATTTGGGGTTTGAAATATTTTGGAAATAATTTTAATAGATTGGTGGATGTTAAGACTAGGTTTGATCCTTATGATTTCTTTAGGCATGAACAAAGCATACCTACCCTCTCAAACGAAAAGGTATTTTACTGA

Coding sequence (CDS)

ATGTCTATTCTTCTATCTTCGTCTTCACCATGGGTTGCATCTAATACTTTTGAAAGACATGAAAATTTTCTTCAATGTCTTTCCCATCATTCTCCAAACATTTCCAAAGTCATTTACACTCCCTTCAACTCCTCTTATTCTTCCATCTTAAACTTCACCCTTAGAAACCTAAGATTCTCAACCCCCAATACCCCAAAGCCACTCCTCATCATCACTCCCTCACATATTTCCCACATCCAAGCCGCCGTCATCTGTTCCAAACCCTACGGTCTTCAAATCCGAACTCGAAGTGGTGGCCATGATTTCGAGGGCCTTTCTTACGTCGCCTACCTTCCATTCGTCATCCTTGACCTAATAAATTTAAGCTCTATCTCCATCGATGTCGAAACTAACACTGCATGGGTTGAGTCCGGTGCAACCATAGGTGAACTTTACTATAGAATCGCCGAGAAAAGTCGAACCTTGGCTTTTCCGGCCGGTACTTGTCCGATAGTCGGCGTTGGCGGGCAGTTCAGTGGCGGCGGATATGGGATGTTGACGAGGAAATATGGTCTTGCTTCTGATAATGTGATAGATGCTTATTTGGTTGATGCAAATGGGGAGTTTCATGATAGAAAGTCAATGGGGGAGGATTTGTTTTGGGCCATTAGAGGCGGCGGGGGAGGGAGCTTTGGAATAGTGGTGGCGTGGAAGGTGAAGCTGGTTCCGGTGCCGGCGACGGTGACGCTTTGCTTTGTTAACAAAAATTCGGAGGAAGGAGCAACCAATCTAATTCATCGGTGGCAATATGTTGCTCACGACTTAGATGAAAATATATTCCTTGGGATTCTTTTGGCTGGTGGGAATTTTACATGGCAAGAAGGCAAAATAAATCCAACAGCTTTATTCCTCTCTTTGTATCTTGGGAAGGTAGAAGAGCTTGTGTCAATCCTCAACACAACTTTTCCTGAATTGGGTTTGGCAAAGAAAGATTGCACAGAAACAAGCTGGGTTGAATCAACCCTCATCATGGGCACTGGCCTCCAACCAGGGAAGGCATTAGAAGATCTACTAAGTAAAGCATCTCTAACCAATGCAAGTACAAAAATCAAATCTGATTACATTAAACAACCCATCCCTATGGCCGCAGTTGAGGGCATATGGCAAAGATTAAAACTTGAAGATATAGAAATGCCACAACTTCTACTTTCTCCTTATGGTGGAAGAATGAGCCAGATTTCTGAGTCAGAAACTCCTTTTTCACATAGGTCGGAATATTTATACACAATTGGTTACGTCATTAATTGGGAACAACAAGGTAATGATGTTGAGAAAAGACACCTAAGTTGGATACGAGAGCTTTATAGTTACATGGCTCCTTTTGCTTCAAAATTACCTAGAGCTGCATATGTCAACTATAGAGACCTTGATATTGGATCAAACTATAGATATGGAAAGACAAGCTACGAGAAAGCTAGCATTTGGGGTTTGAAATATTTTGGAAATAATTTTAATAGATTGGTGGATGTTAAGACTAGGTTTGATCCTTATGATTTCTTTAGGCATGAACAAAGCATACCTACCCTCTCAAACGAAAAGGTATTTTACTGA

Protein sequence

MSILLSSSSPWVASNTFERHENFLQCLSHHSPNISKVIYTPFNSSYSSILNFTLRNLRFSTPNTPKPLLIITPSHISHIQAAVICSKPYGLQIRTRSGGHDFEGLSYVAYLPFVILDLINLSSISIDVETNTAWVESGATIGELYYRIAEKSRTLAFPAGTCPIVGVGGQFSGGGYGMLTRKYGLASDNVIDAYLVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTLCFVNKNSEEGATNLIHRWQYVAHDLDENIFLGILLAGGNFTWQEGKINPTALFLSLYLGKVEELVSILNTTFPELGLAKKDCTETSWVESTLIMGTGLQPGKALEDLLSKASLTNASTKIKSDYIKQPIPMAAVEGIWQRLKLEDIEMPQLLLSPYGGRMSQISESETPFSHRSEYLYTIGYVINWEQQGNDVEKRHLSWIRELYSYMAPFASKLPRAAYVNYRDLDIGSNYRYGKTSYEKASIWGLKYFGNNFNRLVDVKTRFDPYDFFRHEQSIPTLSNEKVFY
BLAST of Lsi02G009770 vs. Swiss-Prot
Match: THCAS_CANSA (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=1 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.5e-148
Identity = 260/511 (50.88%), Postives = 351/511 (68.69%), Query Frame = 1

Query: 21  ENFLQCLSHHSPNI---SKVIYTPFNSSYSSILNFTLRNLRFSTPNTPKPLLIITPSHIS 80
           ENFL+C S H PN     K++YT  +  Y SILN T++NLRF +  TPKPL+I+TPS+ S
Sbjct: 32  ENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPSNNS 91

Query: 81  HIQAAVICSKPYGLQIRTRSGGHDFEGLSYVAYLPFVILDLINLSSISIDVETNTAWVES 140
           HIQA ++CSK  GLQIRTRSGGHD EG+SY++ +PFV++DL N+ SI IDV + TAWVE+
Sbjct: 92  HIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVEA 151

Query: 141 GATIGELYYRIAEKSRTLAFPAGTCPIVGVGGQFSGGGYGMLTRKYGLASDNVIDAYLVD 200
           GAT+GE+YY I EK+  L+FP G CP VGVGG FSGGGYG L R YGLA+DN+IDA+LV+
Sbjct: 152 GATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 201 ANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTLCFVNKNSE-EGATN 260
            +G+  DRKSMGEDLFWAIRGGGG +FGI+ AWK+KLV VP+  T+  V KN E  G   
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHGLVK 271

Query: 261 LIHRWQYVAHDLDENIFLGILLAGGNFTWQEGKINPT--ALFLSLYLGKVEELVSILNTT 320
           L ++WQ +A+  D+++ L       N T   GK   T    F S++ G V+ LV ++N +
Sbjct: 272 LFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNKS 331

Query: 321 FPELGLAKKDCTETSWVESTLIMGTGLQPGKA---LEDLLSKASLTNASTKIKSDYIKQP 380
           FPELG+ K DC E SW+++T+     +    A    E LL +++    +  IK DY+K+P
Sbjct: 332 FPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRSAGKKTAFSIKLDYVKKP 391

Query: 381 IPMAAVEGIWQRLKLEDIEMPQLLLSPYGGRMSQISESETPFSHRSEYLYTIGYVINWEQ 440
           IP  A+  I ++L  ED+     +L PYGG M +ISES  PF HR+  +Y + Y  +WE+
Sbjct: 392 IPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASWEK 451

Query: 441 QGNDVEKRHLSWIRELYSYMAPFASKLPRAAYVNYRDLDIGSNYRYGKTSYEKASIWGLK 500
           Q ++  ++H++W+R +Y++  P+ S+ PR AY+NYRDLD+G        +Y +A IWG K
Sbjct: 452 QEDN--EKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYTQARIWGEK 511

Query: 501 YFGNNFNRLVDVKTRFDPYDFFRHEQSIPTL 523
           YFG NFNRLV VKT+ DP +FFR+EQSIP L
Sbjct: 512 YFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of Lsi02G009770 vs. Swiss-Prot
Match: THCAI_CANSA (Inactive tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=3 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 1.8e-146
Identity = 253/511 (49.51%), Postives = 350/511 (68.49%), Query Frame = 1

Query: 21  ENFLQCLSHHSPNIS---KVIYTPFNSSYSSILNFTLRNLRFSTPNTPKPLLIITPSHIS 80
           ENFL+C S + PN     K IYT  +  Y S+LN T++NLRF++  TPKPL+I+TPS++S
Sbjct: 32  ENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRFTSDTTPKPLVIVTPSNVS 91

Query: 81  HIQAAVICSKPYGLQIRTRSGGHDFEGLSYVAYLPFVILDLINLSSISIDVETNTAWVES 140
           HIQA+++CSK  GLQIRTRSGGHD EGLSY++ +PF I+DL N+ ++ +D+ + TAWVE+
Sbjct: 92  HIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHTVKVDIHSQTAWVEA 151

Query: 141 GATIGELYYRIAEKSRTLAFPAGTCPIVGVGGQFSGGGYGMLTRKYGLASDNVIDAYLVD 200
           GAT+GE+YY I E +   +FP G CP VGVGG FSGGGYG L R YGLA+DN+IDA+LV+
Sbjct: 152 GATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 201 ANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTLCFVNKNSE-EGATN 260
            +G+  DRKSMGEDLFWAIRGGGG +FGI+ AWK+KLV VP+  T+  V KN E  G   
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPSKATIFSVKKNMEIHGLVK 271

Query: 261 LIHRWQYVAHDLDENIFLGILLAGGNFTWQEGKINPT--ALFLSLYLGKVEELVSILNTT 320
           L ++WQ +A+  D+++ L       N T   GK   T    F S++LG V+ LV ++N +
Sbjct: 272 LFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGYFSSIFLGGVDSLVDLMNKS 331

Query: 321 FPELGLAKKDCTETSWVESTLIMGTGLQPGKA---LEDLLSKASLTNASTKIKSDYIKQP 380
           FPELG+ K DC E SW+++T+     +    A    E LL +++    +  IK DY+K+ 
Sbjct: 332 FPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEILLDRSAGKKTAFSIKLDYVKKL 391

Query: 381 IPMAAVEGIWQRLKLEDIEMPQLLLSPYGGRMSQISESETPFSHRSEYLYTIGYVINWEQ 440
           IP  A+  I ++L  E++ +   +L PYGG M +ISES  PF HR+  +Y + Y   WE+
Sbjct: 392 IPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAIPFPHRAGIMYELWYTATWEK 451

Query: 441 QGNDVEKRHLSWIRELYSYMAPFASKLPRAAYVNYRDLDIGSNYRYGKTSYEKASIWGLK 500
           Q ++  ++H++W+R +Y++  P+ S+ PR AY+NYRDLD+G        +Y +A IWG K
Sbjct: 452 QEDN--EKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNPESPNNYTQARIWGEK 511

Query: 501 YFGNNFNRLVDVKTRFDPYDFFRHEQSIPTL 523
           YFG NFNRLV VKT+ DP +FFR+EQSIP L
Sbjct: 512 YFGKNFNRLVKVKTKADPNNFFRNEQSIPPL 540

BLAST of Lsi02G009770 vs. Swiss-Prot
Match: CBDAS_CANSA (Cannabidiolic acid synthase OS=Cannabis sativa GN=CBDAS PE=1 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 6.7e-146
Identity = 263/517 (50.87%), Postives = 356/517 (68.86%), Query Frame = 1

Query: 21  ENFLQCLSHHSPNIS---KVIYTPFNSSYSSILNFTLRNLRFSTPNTPKPLLIITPSHIS 80
           ENFL+C S + PN +   K++YT  N  Y S+LN T+ NLRF++  TPKPL+I+TPSH+S
Sbjct: 32  ENFLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPLVIVTPSHVS 91

Query: 81  HIQAAVICSKPYGLQIRTRSGGHDFEGLSYVAYLPFVILDLINLSSISIDVETNTAWVES 140
           HIQ  ++CSK  GLQIRTRSGGHD EG+SY++ +PFVI+DL N+ SI IDV + TAWVE+
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDVHSQTAWVEA 151

Query: 141 GATIGELYYRIAEKSRTLAFPAGTCPIVGVGGQFSGGGYGMLTRKYGLASDNVIDAYLVD 200
           GAT+GE+YY + EK+  L+  AG CP V  GG F GGGYG L R YGLA+DN+IDA+LV+
Sbjct: 152 GATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPLMRNYGLAADNIIDAHLVN 211

Query: 201 ANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTLCFVNKNSE-EGATN 260
            +G+  DRKSMGEDLFWA+RGGG  SFGI+VAWK++LV VP + T+  V K  E      
Sbjct: 212 VHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVPKS-TMFSVKKIMEIHELVK 271

Query: 261 LIHRWQYVAHDLDENIFLGILLAGGNFTWQEGKINPTAL---FLSLYLGKVEELVSILNT 320
           L+++WQ +A+  D+++ L       N T  +GK N TA+   F S++LG V+ LV ++N 
Sbjct: 272 LVNKWQNIAYKYDKDLLLMTHFITRNITDNQGK-NKTAIHTYFSSVFLGGVDSLVDLMNK 331

Query: 321 TFPELGLAKKDCTETSWVESTLIMGTGL----QPGKALEDLLSKASLTNASTKIKSDYIK 380
           +FPELG+ K DC + SW++ T+I  +G+          E LL +++  N + KIK DY+K
Sbjct: 332 SFPELGIKKTDCRQLSWID-TIIFYSGVVNYDTDNFNKEILLDRSAGQNGAFKIKLDYVK 391

Query: 381 QPIPMAAVEGIWQRLKLEDIEMPQLLLSPYGGRMSQISESETPFSHRSEYLYTIGYVINW 440
           +PIP +    I ++L  EDI      L PYGG M +ISES  PF HR+  LY + Y+ +W
Sbjct: 392 KPIPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIPFPHRAGILYELWYICSW 451

Query: 441 EQQGNDVEKRHLSWIRELYSYMAPFASKLPRAAYVNYRDLDIGSNYRYGKTSYEKASIWG 500
           E+Q ++  ++HL+WIR +Y++M P+ SK PR AY+NYRDLDIG N      +Y +A IWG
Sbjct: 452 EKQEDN--EKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIGINDPKNPNNYTQARIWG 511

Query: 501 LKYFGNNFNRLVDVKTRFDPYDFFRHEQSIPTLSNEK 527
            KYFG NF+RLV VKT  DP +FFR+EQSIP L   +
Sbjct: 512 EKYFGKNFDRLVKVKTLVDPNNFFRNEQSIPPLPRHR 543

BLAST of Lsi02G009770 vs. Swiss-Prot
Match: CASL1_CANSA (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 2.0e-145
Identity = 256/512 (50.00%), Postives = 355/512 (69.34%), Query Frame = 1

Query: 21  ENFLQCLSHHSP-NIS--KVIYTPFNSSYSSILNFTLRNLRFSTPNTPKPLLIITPSHIS 80
           ENFL+C S + P N++  K++YT  +  Y SILN T++NLRF++  TPKPL+IITP ++S
Sbjct: 32  ENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPLNVS 91

Query: 81  HIQAAVICSKPYGLQIRTRSGGHDFEGLSYVAYLPFVILDLINLSSISIDVETNTAWVES 140
           HIQ  ++CSK  GLQIRTRSGGHD EG+SY++ +PFVI+DL N+ S+ IDV + TAWVE+
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVEA 151

Query: 141 GATIGELYYRIAEKSRTLAFPAGTCPIVGVGGQFSGGGYGMLTRKYGLASDNVIDAYLVD 200
           GAT+GE+YY I E +  L+FPAG CP VG GG FSGGGYG L R YGLA+DN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 201 ANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTLCFVNKNSE-EGATN 260
            +G+  DRKSMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  T+  V KN E      
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 261 LIHRWQYVAHDLDENIFLGILLAGGNFTWQEGKINPT--ALFLSLYLGKVEELVSILNTT 320
           L+++WQ +A+  ++ + L       N T  +GK   T  + F S++ G V+ LV ++N +
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 321 FPELGLAKKDCTETSWVESTLIMGTGLQPGKAL----EDLLSKASLTNASTKIKSDYIKQ 380
           FPELG+ K DC + SW++ T+I  +G+          E LL ++    A+  IK DY+K+
Sbjct: 332 FPELGIKKTDCKQLSWID-TIIFYSGVVNYNTTYFKKEILLDRSGGRKAAFSIKLDYVKK 391

Query: 381 PIPMAAVEGIWQRLKLEDIEMPQLLLSPYGGRMSQISESETPFSHRSEYLYTIGYVINWE 440
           PIP  A+  I ++L  ED+ +   +  PYGG M +ISES  PF HR+  +Y I Y+ +WE
Sbjct: 392 PIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIASWE 451

Query: 441 QQGNDVEKRHLSWIRELYSYMAPFASKLPRAAYVNYRDLDIGSNYRYGKTSYEKASIWGL 500
           +Q ++  ++H++WIR +Y++  P+ S+ PR AY+NYRDLD+G        +Y +A IWG 
Sbjct: 452 KQEDN--EKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGE 511

Query: 501 KYFGNNFNRLVDVKTRFDPYDFFRHEQSIPTL 523
           KYFG NFNRLV VKT+ DP +FFR+EQSIP L
Sbjct: 512 KYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Lsi02G009770 vs. Swiss-Prot
Match: CASL2_CANSA (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 3.3e-145
Identity = 257/512 (50.20%), Postives = 354/512 (69.14%), Query Frame = 1

Query: 21  ENFLQCLSHHSP-NIS--KVIYTPFNSSYSSILNFTLRNLRFSTPNTPKPLLIITPSHIS 80
           ENFL+CLS + P N++  K++YT  +  Y SILN T++NLRF++  TPKPL+I TP ++S
Sbjct: 32  ENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTPLNVS 91

Query: 81  HIQAAVICSKPYGLQIRTRSGGHDFEGLSYVAYLPFVILDLINLSSISIDVETNTAWVES 140
           HIQ  ++CSK  GLQIRTRSGGHD EG+SY++ +PFVI+DL N+ S+ IDV + TAWVES
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVES 151

Query: 141 GATIGELYYRIAEKSRTLAFPAGTCPIVGVGGQFSGGGYGMLTRKYGLASDNVIDAYLVD 200
           GAT+GE+YY I E +  L+FPAG CP VG GG FSGGGYG L R YGLA+DN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 201 ANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTLCFVNKNSE-EGATN 260
            +G+  DRKSMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  T+  V KN E      
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 261 LIHRWQYVAHDLDENIFLGILLAGGNFTWQEGKINPT--ALFLSLYLGKVEELVSILNTT 320
           L+++WQ +A+  ++ + L       N T  +GK   T  + F S++ G V+ LV ++N +
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 321 FPELGLAKKDCTETSWVESTLIMGTGL----QPGKALEDLLSKASLTNASTKIKSDYIKQ 380
           FPELG+ K DC + SW++ T+I  +G+          E LL ++    A+  IK DY+K+
Sbjct: 332 FPELGIKKTDCKQLSWID-TIIFYSGVVNYNTTNFKKEILLDRSGGRKAAFSIKLDYVKK 391

Query: 381 PIPMAAVEGIWQRLKLEDIEMPQLLLSPYGGRMSQISESETPFSHRSEYLYTIGYVINWE 440
           PIP  A+  I ++L  ED+ +   +  PYGG M +ISES  PF HR+   Y I Y+ +WE
Sbjct: 392 PIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWYIASWE 451

Query: 441 QQGNDVEKRHLSWIRELYSYMAPFASKLPRAAYVNYRDLDIGSNYRYGKTSYEKASIWGL 500
           +Q ++  ++H++WIR +Y++  P+ S+ PR AY+NYRDLD+G        +Y +A IWG 
Sbjct: 452 KQEDN--EKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGE 511

Query: 501 KYFGNNFNRLVDVKTRFDPYDFFRHEQSIPTL 523
           KYFG NFNRLV VKT+ DP +FFR+EQSIP L
Sbjct: 512 KYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Lsi02G009770 vs. TrEMBL
Match: B9NA97_POPTR (FAD-binding domain-containing family protein OS=Populus trichocarpa GN=POPTR_0001s43120g PE=4 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 3.2e-179
Identity = 311/520 (59.81%), Postives = 394/520 (75.77%), Query Frame = 1

Query: 8   SSPWVASNTFERHENFLQCL-SHHSPNISKVIYTPFNSSYSSILNFTLRNLRFSTPNTPK 67
           S  WV S     HE+FLQCL S +S +ISK+IYTP NSSYSSIL F+++NLRF+T +TPK
Sbjct: 11  SFSWVTSA--HTHESFLQCLDSQNSHSISKLIYTPINSSYSSILQFSIQNLRFNTSSTPK 70

Query: 68  PLLIITPSHISHIQAAVICSKPYGLQIRTRSGGHDFEGLSYVAYLPFVILDLINLSSISI 127
           PL+I+TP++ISHIQAA+ICS+ +G+QIR RSGGHD+EGLSYV+   FV++DLINL +I++
Sbjct: 71  PLVIVTPTNISHIQAAIICSQKHGMQIRIRSGGHDYEGLSYVSTFSFVVIDLINLRTINV 130

Query: 128 DVETNTAWVESGATIGELYYRIAEKSRTLAFPAGTCPIVGVGGQFSGGGYGMLTRKYGLA 187
           + E  TAWV+SG TIGE+YYRIAEKSRTLAFPA  CP VGVGG FSGGGYGML RKYGLA
Sbjct: 131 NAENKTAWVQSGVTIGEVYYRIAEKSRTLAFPASVCPTVGVGGHFSGGGYGMLMRKYGLA 190

Query: 188 SDNVIDAYLVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTLCFV 247
           +D++IDA LVD  G   DR+SMGEDLFWAIRGGGG +FG+V+AWK+ LV  P TVT+  V
Sbjct: 191 ADHIIDAQLVDVKGRILDRESMGEDLFWAIRGGGGNTFGVVIAWKISLVSAPPTVTVFNV 250

Query: 248 NKNSEEGATNLIHRWQYVAHDLDENIFLGILLAGGNFTWQEGKINPT--ALFLSLYLGKV 307
            +  E+ A+ L+HRWQ+VA  L E++F+ I+L   N T +EGK N T  A F SL+LG +
Sbjct: 251 QRTLEQNASKLVHRWQFVADKLHEDLFIRIILNRVNST-EEGKSNTTIQASFNSLFLGGI 310

Query: 308 EELVSILNTTFPELGLAKKDCTETSWVESTLIMGTGLQPGKALEDLLSKASLTNASTKIK 367
           + L+ ++  +FPELGL K+DC E SW++S L    G     +L+ LL +   T  + K K
Sbjct: 311 DRLLPLIQDSFPELGLVKEDCIEMSWIQSVLYF-DGFPSNSSLDVLLDRTPSTRRNFKAK 370

Query: 368 SDYIKQPIPMAAVEGIWQRLKLEDIEMPQLLLSPYGGRMSQISESETPFSHRSEYLYTIG 427
           SDY+K+PIP   +EGIW+R   +DI  P L+ SPYGG+MS+ISES  PF HR+  +Y I 
Sbjct: 371 SDYVKEPIPELGLEGIWERFFDKDINTPILIFSPYGGKMSEISESSIPFPHRAGNIYKIQ 430

Query: 428 YVINWEQQGNDVEKRHLSWIRELYSYMAPFASKLPRAAYVNYRDLDIGSNYRYGKTSYEK 487
           ++I W+++G    KRH+SWIR LYSY+AP+ SK PRAAYVNYRDLDIG N   G TSY +
Sbjct: 431 HLIYWDEEGIVATKRHISWIRRLYSYLAPYVSKTPRAAYVNYRDLDIGINNHAGNTSYRQ 490

Query: 488 ASIWGLKYFGNNFNRLVDVKTRFDPYDFFRHEQSIPTLSN 525
           ASIWGLKYF NNF+RLV VKT  DP +FFR+EQSIP LS+
Sbjct: 491 ASIWGLKYFKNNFDRLVRVKTAVDPANFFRNEQSIPPLSS 526

BLAST of Lsi02G009770 vs. TrEMBL
Match: V4UIF2_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10017782mg PE=4 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 3.0e-177
Identity = 311/532 (58.46%), Postives = 407/532 (76.50%), Query Frame = 1

Query: 1   MSILLSSSSPWVASNTFERHENFLQCLSHHSPN---ISKVIYTPFNSSYSSILNFTLRNL 60
           +++LLS      A++T   HE+FLQCLS HS N   ISKVIYT +NSSYSS+L  TL+NL
Sbjct: 13  LALLLSFQLIAAAADT---HESFLQCLSLHSENSTSISKVIYTHYNSSYSSVLELTLQNL 72

Query: 61  RFSTPNTPKPLLIITPSHISHIQAAVICSKPYGLQIRTRSGGHDFEGLSY-VAY-LPFVI 120
           RFSTP TPKPL+IITP H+SHIQAA+ CS+ YG+QIR RSGGHD+EGLS+  +Y  PFVI
Sbjct: 73  RFSTPATPKPLVIITPIHVSHIQAAIKCSQKYGMQIRVRSGGHDYEGLSFRTSYNAPFVI 132

Query: 121 LDLINLSSISIDVETNTAWVESGATIGELYYRIAEKSRTLAFPAGTCPIVGVGGQFSGGG 180
           +D+INLSSISIDVE  TAWV+ GAT+G+LYYRIAEKS  LAFPAG+CP VG GG  SGGG
Sbjct: 133 IDMINLSSISIDVENKTAWVQVGATLGQLYYRIAEKSPNLAFPAGSCPSVGTGGHISGGG 192

Query: 181 YGMLTRKYGLASDNVIDAYLVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLV 240
           +G+L RKYGLA+DN++DA+++D  G F DRKSMGEDLFWAIRGGGG SFG++VAWK+KLV
Sbjct: 193 WGILLRKYGLAADNIVDAHMIDVQGRFLDRKSMGEDLFWAIRGGGGASFGVIVAWKIKLV 252

Query: 241 PVPATVTLCFVNKNSEEGATNLIHRWQYVAHDLDENIFLGILLAGGNFTWQEGKINPTAL 300
            VP+TVT+  V +  ++  T L+HRWQ+VAH L E++F+ + L   + +  EG     A+
Sbjct: 253 DVPSTVTVFTVRRPWDQIVTKLVHRWQHVAHKLPEDLFIMVFLRSLS-SGTEGNRTMEAI 312

Query: 301 FLSLYLGKVEELVSILNTTFPELGLAKKDCTETSWVESTLIMGTGLQPGKALEDLLSKAS 360
           F SL+LG VE+L+ ++   FPELGL K+DC ETSW++S +I   G + G++L+ LL + +
Sbjct: 313 FQSLFLGGVEKLLPLMQERFPELGLVKEDCNETSWIQSIMIF-NGFESGESLDILLQRTN 372

Query: 361 LTN-ASTKIKSDYIKQPIPMAAVEGIWQRLKLEDIEMPQLLLSPYGGRMSQISESETPFS 420
            ++  + K KSDY+++P+P  A+EGI++RL   + E  QL   PYGG+MS+ISES  PF 
Sbjct: 373 YSDRRAFKAKSDYVREPMPGIALEGIYERLSKVEAEAAQLFFVPYGGKMSEISESAIPFP 432

Query: 421 HRSEYLYTIGYVINWEQQGNDVEKRHLSWIRELYSYMAPFASKLPRAAYVNYRDLDIGSN 480
           HR+  +Y IG+++ W + G D  +RH++WIRELYSYM P+ SK PRAAY+NYRDLDIG+N
Sbjct: 433 HRAGNIYEIGHLVLWAEDGIDASQRHINWIRELYSYMTPYVSKNPRAAYINYRDLDIGTN 492

Query: 481 YRYGKTSYEKASIWGLKYFGNNFNRLVDVKTRFDPYDFFRHEQSIPTLSNEK 527
              G TSY++AS+WG KYF +NF+RLV VKT  DP +FFR+EQSIP LS+ K
Sbjct: 493 SE-GYTSYKEASVWGKKYFNHNFDRLVHVKTSVDPDNFFRNEQSIPPLSSWK 538

BLAST of Lsi02G009770 vs. TrEMBL
Match: A0A067GQ97_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g009115mg PE=4 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 1.1e-176
Identity = 311/532 (58.46%), Postives = 407/532 (76.50%), Query Frame = 1

Query: 1   MSILLSSSSPWVASNTFERHENFLQCLSHHSPN---ISKVIYTPFNSSYSSILNFTLRNL 60
           +++LLS      A++T   HE+FLQCLS HS N   ISKVIYT +NSSYSS+L  TL+NL
Sbjct: 13  LALLLSFQLIAAAADT---HESFLQCLSLHSENSTSISKVIYTQYNSSYSSVLELTLQNL 72

Query: 61  RFSTPNTPKPLLIITPSHISHIQAAVICSKPYGLQIRTRSGGHDFEGLSY-VAY-LPFVI 120
           RFSTP TPKPL+IITP H+SHIQAA+ CS+ YG+QIR RSGGHD+EGLS+  +Y  PFVI
Sbjct: 73  RFSTPATPKPLVIITPIHVSHIQAAIKCSQKYGMQIRVRSGGHDYEGLSFRTSYNSPFVI 132

Query: 121 LDLINLSSISIDVETNTAWVESGATIGELYYRIAEKSRTLAFPAGTCPIVGVGGQFSGGG 180
           +D+INLSSISIDVE  TAWV+ GAT+G+LYYRIAEKS  LAFPAG+CP VG GG  SGGG
Sbjct: 133 IDMINLSSISIDVENKTAWVQVGATLGQLYYRIAEKSPNLAFPAGSCPSVGTGGHISGGG 192

Query: 181 YGMLTRKYGLASDNVIDAYLVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLV 240
           +G+L RKYGLA+DN++DA ++D  G F DRKSMGEDLFWAIRGGGG SFG++VAWK+KLV
Sbjct: 193 WGILLRKYGLAADNIVDARMIDVQGRFLDRKSMGEDLFWAIRGGGGASFGVIVAWKIKLV 252

Query: 241 PVPATVTLCFVNKNSEEGATNLIHRWQYVAHDLDENIFLGILLAGGNFTWQEGKINPTAL 300
            VP+TVT+  V +  ++ AT L+HRWQ+VAH L E++F+ + L   + +  EG     A+
Sbjct: 253 DVPSTVTVFTVRRPWDQIATKLVHRWQHVAHKLPEDLFIMVFLRSLS-SGTEGNRTMEAI 312

Query: 301 FLSLYLGKVEELVSILNTTFPELGLAKKDCTETSWVESTLIMGTGLQPGKALEDLLSKAS 360
           F SL+LG VE+L+ ++   FPELGL K+DC ETSW++S +I   G + G++L+ LL + +
Sbjct: 313 FQSLFLGGVEKLLPLMQERFPELGLVKEDCNETSWIQSIMIF-NGFESGESLDILLQRTN 372

Query: 361 LTN-ASTKIKSDYIKQPIPMAAVEGIWQRLKLEDIEMPQLLLSPYGGRMSQISESETPFS 420
            ++  + K KSDY+++P+P  A+EGI++RL   + E  QL   PYGG+MS+IS+S  PF 
Sbjct: 373 YSDRRAFKAKSDYVREPMPGIALEGIYERLSKVEAEAAQLFFVPYGGKMSEISKSAIPFP 432

Query: 421 HRSEYLYTIGYVINWEQQGNDVEKRHLSWIRELYSYMAPFASKLPRAAYVNYRDLDIGSN 480
           HR+  +Y IG+++ W + G D  +RH++WIRELYSYM P+ SK PRAAY+NYRDLDIG+N
Sbjct: 433 HRAGNIYEIGHLVLWAEDGIDASQRHINWIRELYSYMTPYVSKNPRAAYINYRDLDIGTN 492

Query: 481 YRYGKTSYEKASIWGLKYFGNNFNRLVDVKTRFDPYDFFRHEQSIPTLSNEK 527
              G TSY++AS+WG KYF +NF+RLV VKT  DP +FFR+EQSIP LS+ K
Sbjct: 493 -NEGYTSYKEASVWGKKYFNHNFDRLVHVKTSVDPDNFFRNEQSIPPLSSWK 538

BLAST of Lsi02G009770 vs. TrEMBL
Match: V4W7E3_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10018353mg PE=4 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 2.6e-176
Identity = 307/515 (59.61%), Postives = 398/515 (77.28%), Query Frame = 1

Query: 18  ERHENFLQCLSHHSPN---ISKVIYTPFNSSYSSILNFTLRNLRFSTPNTPKPLLIITPS 77
           + HE+FLQCLS HS N   ISKVIYT +NSSYSS+L  TL+NLRFSTP TPKPL+IITP 
Sbjct: 27  DTHESFLQCLSLHSENSTSISKVIYTHYNSSYSSVLELTLQNLRFSTPATPKPLVIITPI 86

Query: 78  HISHIQAAVICSKPYGLQIRTRSGGHDFEGLSY-VAY-LPFVILDLINLSSISIDVETNT 137
           H+S IQAA+ CS+ YG+QIR RSGGHD+EGLS+  +Y  PFVI+D+INLSSISIDVE  T
Sbjct: 87  HLSDIQAAIKCSQKYGMQIRVRSGGHDYEGLSFRTSYNSPFVIIDMINLSSISIDVENKT 146

Query: 138 AWVESGATIGELYYRIAEKSRTLAFPAGTCPIVGVGGQFSGGGYGMLTRKYGLASDNVID 197
           AWV+ GAT+G+LYYRIAEKS  LAFPAG+CP VG GG  SGGG+G+L RKYGLA+DN+ID
Sbjct: 147 AWVQVGATLGQLYYRIAEKSPNLAFPAGSCPSVGTGGHISGGGWGILLRKYGLAADNIID 206

Query: 198 AYLVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTLCFVNKNSEE 257
           A+++D  G F DRKSMGEDLFWAIRGGGG SFG++VAWK+KLV VP+TVT+  V +  ++
Sbjct: 207 AHMIDVQGRFLDRKSMGEDLFWAIRGGGGASFGVIVAWKIKLVDVPSTVTVFTVRRPWDQ 266

Query: 258 GATNLIHRWQYVAHDLDENIFLGILLAGGNFTWQEGKINPTALFLSLYLGKVEELVSILN 317
            AT L+HRWQ+VAH L E++F+ + L   + +  EG     A+F SL+LG VE+L+ ++ 
Sbjct: 267 IATKLVHRWQHVAHKLPEDLFIMVFLRSLS-SGTEGNRTMEAIFQSLFLGGVEKLLPLMQ 326

Query: 318 TTFPELGLAKKDCTETSWVESTLIMGTGLQPGKALEDLLSKASLTN-ASTKIKSDYIKQP 377
             FPELGL K+DC ETSW++S +I   G + G++L+ LL + + ++  + K KSDY+++P
Sbjct: 327 ERFPELGLVKEDCNETSWIQSIMIF-NGFESGESLDILLQRTNYSDRRAFKAKSDYVREP 386

Query: 378 IPMAAVEGIWQRLKLEDIEMPQLLLSPYGGRMSQISESETPFSHRSEYLYTIGYVINWEQ 437
           +P  A+EGI++RL   + E  QL   PYGG+MS+ISES  PF HR+  +Y IG+++ W +
Sbjct: 387 MPGIALEGIYERLSKVEAEAAQLFFVPYGGKMSEISESAIPFPHRAGNIYEIGHLVLWAE 446

Query: 438 QGNDVEKRHLSWIRELYSYMAPFASKLPRAAYVNYRDLDIGSNYRYGKTSYEKASIWGLK 497
            G D  +RH++WIRELYSYM P+ SK PRAAY+NYRDLDIG+N   G TSY++AS+WG K
Sbjct: 447 DGIDASQRHINWIRELYSYMTPYVSKNPRAAYINYRDLDIGTN-NEGYTSYKEASVWGKK 506

Query: 498 YFGNNFNRLVDVKTRFDPYDFFRHEQSIPTLSNEK 527
           YF +NF+RLV VKT  DP +FFR+EQSIP LS+ K
Sbjct: 507 YFNHNFDRLVHVKTSVDPDNFFRNEQSIPPLSSWK 538

BLAST of Lsi02G009770 vs. TrEMBL
Match: A0A059CBE6_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D00070 PE=4 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 5.7e-176
Identity = 303/527 (57.50%), Postives = 388/527 (73.62%), Query Frame = 1

Query: 1   MSILLSSSSPWVASNTFERHENFLQCLSHHSPN---ISKVIYTPFNSSYSSILNFTLRNL 60
           ++ LL  ++P VA +     ENFLQCLS HS N   ISK++YTP NSSYSS+L+ T++NL
Sbjct: 12  LAFLLLFAAPLVAFD-INTPENFLQCLSLHSRNASAISKLVYTPINSSYSSVLDITVQNL 71

Query: 61  RFSTPNTPKPLLIITPSHISHIQAAVICSKPYGLQIRTRSGGHDFEGLSYVAYLPFVILD 120
           RF TP+ PKP  I+ P  +SH+QA +ICS+ YGLQ+RTRSGGHD+EGLSY++ +PFVI+D
Sbjct: 72  RFLTPSAPKPSFIVMPLDVSHVQATIICSRQYGLQMRTRSGGHDYEGLSYLSEVPFVIVD 131

Query: 121 LINLSSISIDVETNTAWVESGATIGELYYRIAEKSRTLAFPAGTCPIVGVGGQFSGGGYG 180
           + N  SIS+DVE  TAWV+SG T+GELYYRIAEKS+TL FPAG CP VG+GG FSGGGYG
Sbjct: 132 MANFRSISVDVEDKTAWVQSGVTVGELYYRIAEKSKTLGFPAGICPTVGIGGHFSGGGYG 191

Query: 181 MLTRKYGLASDNVIDAYLVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPV 240
            L RKYGLA+DNVID  LVDA G   DR SMGEDLFWAIRGGGGGSFG+VV+WKVKLVPV
Sbjct: 192 TLLRKYGLAADNVIDVQLVDAQGRLQDRVSMGEDLFWAIRGGGGGSFGVVVSWKVKLVPV 251

Query: 241 PATVTLCFVNKNSEEGATNLIHRWQYVAHDLDENIFLGILLAGGNFTWQEGKINPTALFL 300
           P+TVT+  + +  E+ AT L+H+WQ VA  L  ++F+ ++L   N +   G     A+F 
Sbjct: 252 PSTVTVFTILRTLEQNATKLVHQWQLVASKLPNDLFIRLILQSYN-SGPNGTRTMQAIFN 311

Query: 301 SLYLGKVEELVSILNTTFPELGLAKKDCTETSWVESTLIMGTGLQPGKALEDLLSKASLT 360
           +LYLG V+ L+ +  + FPEL + ++DCTE SW+ES L   TG   G  LE LL+++S T
Sbjct: 312 TLYLGGVDSLLEVTRSRFPELNVTRQDCTEMSWIESVLYF-TGFPSGTPLEVLLNRSSNT 371

Query: 361 NASTKIKSDYIKQPIPMAAVEGIWQRLKLEDIEMPQLLLSPYGGRMSQISESETPFSHRS 420
               K KSDY+K+PIP + +EG+W+R   ED  +P ++ +PYGG+M++ISES  PF HR+
Sbjct: 372 KTFFKNKSDYVKEPIPESGLEGMWERFFEEDQGVPLMIWNPYGGKMAEISESAIPFPHRA 431

Query: 421 EYLYTIGYVINWEQQGNDVEKRHLSWIRELYSYMAPFASKLPRAAYVNYRDLDIGSNYRY 480
             LY I ++  W   GN+V  +H+SWIR  YSYMA + SK PR AY+NYRDLDIG N   
Sbjct: 432 GVLYKIQHITAWTTGGNEVAPKHVSWIRSFYSYMAQYVSKSPREAYINYRDLDIGMNNVI 491

Query: 481 GKTSYEKASIWGLKYFGNNFNRLVDVKTRFDPYDFFRHEQSIPTLSN 525
           G TSY++AS+WG KYF NNF+RLV VKT  DP +FFRHEQSIP  S+
Sbjct: 492 GDTSYKQASVWGKKYFKNNFDRLVRVKTAVDPDNFFRHEQSIPPFSS 535

BLAST of Lsi02G009770 vs. TAIR10
Match: AT4G20820.1 (AT4G20820.1 FAD-binding Berberine family protein)

HSP 1 Score: 521.2 bits (1341), Expect = 7.7e-148
Identity = 257/522 (49.23%), Postives = 363/522 (69.54%), Query Frame = 1

Query: 2   SILLSSSSPWVASNTFERHENFLQCLS---HHSPNISKVIYTPFNSSYSSILNFTLRNLR 61
           ++LLS   P  A+ +     +FLQCLS   + S  +SKVI+TP ++S+SS+L  +++N R
Sbjct: 18  TLLLSIPHPVSANRS--NQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQR 77

Query: 62  FSTPNTPKPLLIITPSHISHIQAAVICSKPYGLQIRTRSGGHDFEGLSYVAYLPFVILDL 121
           FS P+ PKP+LI+TP   S +Q+AV C++ +G+ IRTRSGGHD+EGLSYV + PFVILDL
Sbjct: 78  FSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDL 137

Query: 122 INLSSISIDVETNTAWVESGATIGELYYRIAEKSRTLAFPAGTCPIVGVGGQFSGGGYGM 181
            NL SI++DV+  + WV++GATIGELYY I +K+RTLAFPAG CP VGVGG FSGGGYG 
Sbjct: 138 RNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGT 197

Query: 182 LTRKYGLASDNVIDAYLVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVP 241
           L RK+GLA+D+VIDA +VDA G   +R+ MGED FWAIRGGGG SF +V++WK+ L+ VP
Sbjct: 198 LLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVP 257

Query: 242 ATVTLCFVNKNSEEGATNLIHRWQYVAHDLDENIFLGILLAGGNFTWQEGKINPTALFLS 301
           +TVT+  V K SE+ A  +IHRWQ+VA  + +++F+ ++L       Q  K    A F  
Sbjct: 258 STVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML-------QRYKNMVRASFPG 317

Query: 302 LYLGKVEELVSILNTTFPELGLAKKDCTETSWVESTLIMGTGLQPGKALEDLLSKASLTN 361
           LYLG V+ L+ ++N  FPELGL + DCTE SW+ES +      + G+   ++L+K +  +
Sbjct: 318 LYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFA---ELGEEPINVLTKRTRAS 377

Query: 362 ASTKIKSDYIKQPIPMAAVEGIWQRLKLEDIEMPQLLLSPYGGRMSQISESETPFSHRSE 421
            + K KSD++++P+P  A+  +W+RL+  + E  QL+ +P+GG+MS+I++ ETPF HR  
Sbjct: 378 LAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKG 437

Query: 422 YLYTIGYVINWEQQGNDVEKRHLSWIRELYSYMAPFASKLPRAAYVNYRDLDIGSNYRYG 481
            +Y I Y+  W     DV+++++ W+  +Y  M+ F +K PR AY+N RDLD+G      
Sbjct: 438 NIYEIQYLNYWR---GDVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVGVK 497

Query: 482 KTSYEKASIWGLKYFGNNFNRLVDVKTRFDPYDFFRHEQSIP 521
           ++ YE+   WG+KYF NNF RLV VKT  DP DFF  EQSIP
Sbjct: 498 RSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of Lsi02G009770 vs. TAIR10
Match: AT5G44440.1 (AT5G44440.1 FAD-binding Berberine family protein)

HSP 1 Score: 505.8 bits (1301), Expect = 3.3e-143
Identity = 254/522 (48.66%), Postives = 357/522 (68.39%), Query Frame = 1

Query: 9   SPWVASNTFERHENFLQCLSHHSPNIS---KVIYTPFNSSYSSILNFTLRNLRFSTPNTP 68
           SP V+++    HE+FL+CLS+   + +   KVI+T  +SS+ SIL+ +++N RFS   TP
Sbjct: 18  SPLVSAHG-SNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSIQNPRFSVSETP 77

Query: 69  KPLLIITPSHISHIQAAVICSKPYGLQIRTRSGGHDFEGLSYVAY-LPFVILDLINLSSI 128
           KP+ IITP   S +Q  + C++ +G+ +RTRS GH +EGLSY+AY  PF ++DL NL SI
Sbjct: 78  KPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVIDLRNLRSI 137

Query: 129 SIDVETNTAWVESGATIGELYYRIAEKSRTLAFPAGTCPIVGVGGQFSGGGYGMLTRKYG 188
           S+DV+  T WV++GAT GELYY I + +++LAFPAG  P VGVGGQFSGGGYG L RKYG
Sbjct: 138 SLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGYGTLLRKYG 197

Query: 189 LASDNVIDAYLVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTLC 248
           LA+DN+IDA +VDA+G   DR++MGED FWAIRGGGG SFG++++WKVKLV VP+T+T+ 
Sbjct: 198 LAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVPSTITVF 257

Query: 249 FVNKNSEEGATNLIHRWQYVAHDLDENIFLGILLAGGNFTWQEGKINPTALFLSLYLGKV 308
            V K S++ A  +I +WQY A  + +++F+   L   N      K    ALF  LY+G V
Sbjct: 258 KVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSN------KNAVHALFTGLYIGPV 317

Query: 309 EELVSILNTTFPELGLAKKDCTETSWVESTLIMGTGLQPGKALEDLLSKASLTNASTKIK 368
             L++++   FPELGL K+ C E SW+ES L        G++L  +L+    T+ S K K
Sbjct: 318 NNLLALMEEKFPELGLEKEGCEEMSWIESVLWF-ADFPKGESL-GVLTNRERTSLSFKGK 377

Query: 369 SDYIKQPIPMAAVEGIWQRLKLEDIEMPQLLLSPYGGRMSQISESETPFSHRSEYLYTIG 428
            D++++PIP AA++ IW+RL+  +  + +++L+P+GG+MS+++E ETPF HR   LY I 
Sbjct: 378 DDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPFPHRGGNLYEIQ 437

Query: 429 YVINW--EQQGNDVE-KRHLSWIRELYSYMAPFASKLPRAAYVNYRDLDIGSNYRYGKTS 488
           YV  W  E+  N  E  ++L W+  +Y +M P+ SK PR AYVN++D+D+G      KT 
Sbjct: 438 YVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDLGMYLGKKKTK 497

Query: 489 YEKASIWGLKYFGNNFNRLVDVKTRFDPYDFFRHEQSIPTLS 524
           YE+   WG+KYF NNF RLV VKTR DP DFF  EQSIP ++
Sbjct: 498 YEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIPLVN 530

BLAST of Lsi02G009770 vs. TAIR10
Match: AT5G44410.1 (AT5G44410.1 FAD-binding Berberine family protein)

HSP 1 Score: 505.0 bits (1299), Expect = 5.7e-143
Identity = 247/509 (48.53%), Postives = 354/509 (69.55%), Query Frame = 1

Query: 20  HENFLQCLSHH-SPNISKVIYTPFNSSYSSILNFTLRNLRFSTPNTPKPLLIITPSHISH 79
           HENFL+CLSH  + + S++I+T  + SY SILN +++N RF    TPKP+ IITP   + 
Sbjct: 34  HENFLKCLSHRINEDDSRIIHTSKDPSYFSILNSSIQNPRFFVLETPKPVSIITPVQATD 93

Query: 80  IQAAVICSKPYGLQIRTRSGGHDFEGLSYVAYL-PFVILDLINLSSISIDVETNTAWVES 139
           +Q+ + C++ +G+ IRTRSGGHD+EGLSY+A   PFV++DL NL SI++DV+  T WV+S
Sbjct: 94  VQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDVDNRTGWVQS 153

Query: 140 GATIGELYYRIAEKSRTLAFPAGTCPIVGVGGQFSGGGYGMLTRKYGLASDNVIDAYLVD 199
           GATIGELYY I + S++LAFPAG  P VG+GGQF GGGYG L RKYGL++DNVIDA++VD
Sbjct: 154 GATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSADNVIDAHIVD 213

Query: 200 ANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTLCFVNKNSEEGATNL 259
           ANG F DR+ MGED FWAIRGGGG SF +V++WK++L+ VP+ VT+  V K SE+ A ++
Sbjct: 214 ANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVKTSEKEAVSI 273

Query: 260 IHRWQYVAHDLDENIFLGILLAGGNFTWQEGKINPTALFLSLYLGKVEELVSILNTTFPE 319
           I++WQY+A  +  ++F+  +L        + +    A F  LYLG V +L++++   FPE
Sbjct: 274 INKWQYIADKVPNDLFIRAML--------QKETEVYASFPGLYLGPVSDLLALMKDKFPE 333

Query: 320 LGLAKKDCTETSWVESTLIMGTGLQPGKALEDLLSKASLTNASTKIKSDYIKQPIPMAAV 379
           LGL   +C E SW+ES L        G+++E +L+K   T+ S K K D+I++PIP  A+
Sbjct: 334 LGLEIGNCREMSWIESVL----WFIKGESME-ILAKRKRTSRSFKGKDDFIEEPIPKTAI 393

Query: 380 EGIWQRLKLEDIEMPQLLLSPYGGRMSQISESETPFSHRSEYLYTIGYVINW---EQQGN 439
           + +W+R +  +  + +++L+P+GG+MS+I+++E PF HR   LY I Y+  W   E +  
Sbjct: 394 QYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYLAYWSEEEDKNK 453

Query: 440 DVEKRHLSWIRELYSYMAPFASKLPRAAYVNYRDLDIGSNYRYG-KTSYEKASIWGLKYF 499
              +++L W+  +Y +M P+ SK PR AYVN+RD+D+G       KT YE+A +WG+KYF
Sbjct: 454 TNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMKTKYEEAKVWGVKYF 513

Query: 500 GNNFNRLVDVKTRFDPYDFFRHEQSIPTL 523
            NNF+RLV VKT  DP DFF  EQSIP +
Sbjct: 514 KNNFDRLVRVKTNVDPMDFFCDEQSIPIM 529

BLAST of Lsi02G009770 vs. TAIR10
Match: AT1G30700.1 (AT1G30700.1 FAD-binding Berberine family protein)

HSP 1 Score: 500.4 bits (1287), Expect = 1.4e-141
Identity = 253/518 (48.84%), Postives = 346/518 (66.80%), Query Frame = 1

Query: 11  WVASNTFERHENFLQCLSHHSPN---ISKVIYTPFNSSYSSILNFTLRNLRFSTPNTPKP 70
           W +S++    E F QCL+ +S     IS  I+   N SYSS+L   +RNLRF+T +TPKP
Sbjct: 16  WQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIRNLRFNTTSTPKP 75

Query: 71  LLIITPSHISHIQAAVICSKPYGLQIRTRSGGHDFEGLSYVAYL--PFVILDLINLSSIS 130
            LII  +H SH+QAA+ C K + LQ++ RSGGHD++GLSYV Y   PF +LD+ NL S+ 
Sbjct: 76  FLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFFVLDMFNLRSVD 135

Query: 131 IDVETNTAWVESGATIGELYYRIAEKSRTLAFPAGTCPIVGVGGQFSGGGYGMLTRKYGL 190
           +DV + TAWV++GA +GE+YY I EKS+TLA+PAG CP VGVGG  SGGGYG + RKYGL
Sbjct: 136 VDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISGGGYGNMMRKYGL 195

Query: 191 ASDNVIDAYLVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTLCF 250
             DN IDA +VD NG+  DRK MGEDL+WAI GGGGGS+G+V+A+K+ LV VP  VT+  
Sbjct: 196 TVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKINLVEVPENVTVFR 255

Query: 251 VNKNSEEGATNLIHRWQYVAHDLDENIFLGILLAGGNFTWQEGKINPTALFLSLYLGKVE 310
           +++  E+ AT++IHRWQ VA  L + +F+  ++   N T    K   T  F++++LG   
Sbjct: 256 ISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRTT-FIAMFLGDTT 315

Query: 311 ELVSILNTTFPELGLAKKDCTETSWVESTLIMGTGLQPGKALEDLLSKASLTNASTKIKS 370
            L+SILN  FPELGL + DCTETSW++S L   T +Q G +   LL +    N   K KS
Sbjct: 316 TLLSILNRRFPELGLVRSDCTETSWIQSVLFW-TNIQVGSSETLLLQRNQPVNY-LKRKS 375

Query: 371 DYIKQPIPMAAVEGIWQRLKLEDIEMPQLLLSPYGGRMSQISESETPFSHRSEYLYTIGY 430
           DY+++PI    +E IW+  K+ ++E+P +  +PYGG M +IS + TPF +R+  L+ I Y
Sbjct: 376 DYVREPISRTGLESIWK--KMIELEIPTMAFNPYGGEMGRISSTVTPFPYRAGNLWKIQY 435

Query: 431 VINWEQQGNDVEKRHLSWIRELYSYMAPFASKLPRAAYVNYRDLDIGSNYRYGK-TSYEK 490
             NW  +   +  R++   R+LY +M PF SK PR ++ NYRD+D+G N   GK +SY +
Sbjct: 436 GANWRDE--TLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHNGKISSYVE 495

Query: 491 ASIWGLKYFGNNFNRLVDVKTRFDPYDFFRHEQSIPTL 523
              +G KYF  NF RLV +KTR D  +FFR+EQSIP L
Sbjct: 496 GKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVL 526

BLAST of Lsi02G009770 vs. TAIR10
Match: AT2G34790.1 (AT2G34790.1 FAD-binding Berberine family protein)

HSP 1 Score: 500.0 bits (1286), Expect = 1.8e-141
Identity = 250/525 (47.62%), Postives = 357/525 (68.00%), Query Frame = 1

Query: 4   LLSSSSPWVASNTFERHENFLQCLSHHSP---NISKVIYTPFNSS--YSSILNFTLRNLR 63
           LL  S P ++S+T +  ++F++CL  +S     I+   ++P  ++  +   L  T +NLR
Sbjct: 17  LLLISVP-LSSSTLQ--QDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTAQNLR 76

Query: 64  FSTPNTPKPLLIITPSHISHIQAAVICSKPYGLQIRTRSGGHDFEGLSYVAY--LPFVIL 123
           + TP+ PKP+ I  P + +H+QAAV+C+K   L +R RSGGHD+EGLS+VA    PFVI+
Sbjct: 77  YLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPFVIV 136

Query: 124 DLINLSSISIDVETNTAWVESGATIGELYYRIAEKSRTLAFPAGTCPIVGVGGQFSGGGY 183
           DL  L  + +D+++N+AW  +GATIGE+YYRI EKS+T  FPAG C  +G+GG   GG Y
Sbjct: 137 DLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGGAY 196

Query: 184 GMLTRKYGLASDNVIDAYLVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVP 243
           G + RK+GL +DNV+DA +VDANG+  DR +MGED+FWAIRGGGGGSFG+++AWK+KLVP
Sbjct: 197 GSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVP 256

Query: 244 VPATVTLCFVNKNSEEGATNLIHRWQYVAHDLDENIFLGILLAGGNFTWQEGKINPTALF 303
           VPATVT+  V K  E+  T ++++W+ +A  LD+++F+ ++++  + T + G    +  +
Sbjct: 257 VPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTISMSY 316

Query: 304 LSLYLGKVEELVSILNTTFPELGLAKKDCTETSWVESTLIMGTGLQPGKALEDLLSKASL 363
            + +LG    L+ ++  +FPELGL KKDCTE SW++S + +  G     A E LL+  SL
Sbjct: 317 QAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYI-AGFPNSAAPEALLAGKSL 376

Query: 364 TNASTKIKSDYIKQPIPMAAVEGIWQRLKLEDIEMPQLLLSPYGGRMSQISESETPFSHR 423
                K KSD++K+PIP+  +EG+W+R   ED   P  + +PYGG MS+ISESE PF HR
Sbjct: 377 FKNHFKAKSDFVKEPIPVEGLEGLWERFLEED--SPLTIWNPYGGMMSRISESEIPFPHR 436

Query: 424 SEYLYTIGYVINWEQQGNDVEKRHLSWIRELYSYMAPFASKLPRAAYVNYRDLDIGSNYR 483
           +  L+ I ++  W Q G   E+RH+ WIRE+YSYM  + SK PR AYVNYRDLD+G+N  
Sbjct: 437 NGTLFKIQWLSTW-QDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTN-- 496

Query: 484 YGKTSYEKASIWGLKYFGNNFNRLVDVKTRFDPYDFFRHEQSIPT 522
            G+T    A  WG KY+  NF RLV +K  FDP +FFRHEQS+PT
Sbjct: 497 EGETD---AREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPT 529

BLAST of Lsi02G009770 vs. NCBI nr
Match: gi|659068687|ref|XP_008445684.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 782.3 bits (2019), Expect = 5.2e-223
Identity = 370/522 (70.88%), Postives = 439/522 (84.10%), Query Frame = 1

Query: 4   LLSSSSPWVASNTFERHENFLQCLSHHSPN---ISKVIYTPFNSSYSSILNFTLRNLRFS 63
           ++ SS+   AS + ++++NFLQCLS HS     ISKV+YTP NSSYSS+L+F++RNL FS
Sbjct: 15  IVVSSASLCASASPQKYQNFLQCLSEHSSKSYPISKVVYTPINSSYSSVLDFSIRNLLFS 74

Query: 64  TPNTPKPLLIITPSHISHIQAAVICSKPYGLQIRTRSGGHDFEGLSYVAYLPFVILDLIN 123
            P TPKPLLIITPSH+SHIQAAVICSK +GLQIRTRSGGHDFEGLSYVAY PF+++DLIN
Sbjct: 75  KPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYRPFIVVDLIN 134

Query: 124 LSSISIDVETNTAWVESGATIGELYYRIAEKSRTLAFPAGTCPIVGVGGQFSGGGYGMLT 183
           L S+ ++VE NTAWVESGAT+GELYYRI EKSRTLAFPAG CP VG+GG FSGGGYG++ 
Sbjct: 135 LRSVKVEVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVGIGGHFSGGGYGLML 194

Query: 184 RKYGLASDNVIDAYLVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPAT 243
           RK+GLA+DNVIDAYLVDANG+  D++SMGEDLFWAIRGGGGGSFGIVVAWK+KLVPVP T
Sbjct: 195 RKFGLAADNVIDAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFGIVVAWKIKLVPVPET 254

Query: 244 VTLCFVNKNSEEGATNLIHRWQYVAHDLDENIFLGILLAGGNFTWQEGKINPTALFLSLY 303
           VT+C  ++N EE A  LIHRWQYV + LDENI+LGI+L GGN + Q G  NPTA F SL+
Sbjct: 255 VTICSTDRNLEEDAIKLIHRWQYVGNKLDENIYLGIILTGGNASTQAGITNPTARFFSLF 314

Query: 304 LGKVEELVSILNTTFPELGLAKKDCTETSWVESTLIMGTGLQPGKALEDLLSKASLTNAS 363
           LG+ +ELV+ L+T FPELGL K+DC E SWVESTLI+  G+QP + LE LL++   T  S
Sbjct: 315 LGRADELVTTLSTIFPELGLIKQDCVEASWVESTLIIPIGVQPIETLEPLLNRTLTTLDS 374

Query: 364 TKIKSDYIKQPIPMAAVEGIWQRLKLEDIEMPQLLLSPYGGRMSQISESETPFSHRSEYL 423
           TKIKSDYI +PIP+AA++ IWQRLK +DIE  QL+  PYGGRMSQISESETPFSHR+ YL
Sbjct: 375 TKIKSDYITEPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRMSQISESETPFSHRARYL 434

Query: 424 YTIGYVINWEQQGNDVEKRHLSWIRELYSYMAPFASKLPRAAYVNYRDLDIGSNYRYGKT 483
           + IGYV+ W+ Q    +KRH+SW+RE+Y YMAPF SK PRAAY NYRDLDIGSN +YGKT
Sbjct: 435 FKIGYVVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYTNYRDLDIGSNNKYGKT 494

Query: 484 SYEKASIWGLKYFGNNFNRLVDVKTRFDPYDFFRHEQSIPTL 523
           SY++AS+WGLKYFGNNF+RLV VKT+ DPYDFFRHEQSIPTL
Sbjct: 495 SYKRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of Lsi02G009770 vs. NCBI nr
Match: gi|778661997|ref|XP_004151192.2| (PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 771.9 bits (1992), Expect = 7.1e-220
Identity = 372/524 (70.99%), Postives = 435/524 (83.02%), Query Frame = 1

Query: 3   ILLSSSSPWVASNTFERHENFLQCLSHHSPN---ISKVIYTPFNSSYSSILNFTLRNLRF 62
           I++SSSS WV+++   +H NFLQCLS HS     ISKV++TP NSSYSS+LNF++RNLRF
Sbjct: 15  IVVSSSSLWVSASP-HKHHNFLQCLSEHSSKSYPISKVVHTPINSSYSSVLNFSIRNLRF 74

Query: 63  STPNTPKPLLIITPSHISHIQAAVICSKPYGLQIRTRSGGHDFEGLSYVAYLPFVILDLI 122
           S P TPKPLLIITPSH+SHI+AAVICSK +GLQIRTRSGGHD+EGLSYVA  PF+ILDLI
Sbjct: 75  SKPETPKPLLIITPSHVSHIRAAVICSKSHGLQIRTRSGGHDYEGLSYVASHPFIILDLI 134

Query: 123 NLSSISIDVETNTAWVESGATIGELYYRIAEKSRTLAFPAGTCPIVGVGGQFSGGGYGML 182
           NL S+ IDVE+NTAWVESG T+GELYYRI EKSRTLAFPAG CP VGVGG FSGGGYG++
Sbjct: 135 NLRSMKIDVESNTAWVESGTTLGELYYRIGEKSRTLAFPAGICPTVGVGGHFSGGGYGLM 194

Query: 183 TRKYGLASDNVIDAYLVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPA 242
            RK+GLA+DNVIDAYLVDANG+ HDR+SMGEDLFWAIRGGGGGSFGIVVAWK+KLV VPA
Sbjct: 195 LRKFGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKIKLVSVPA 254

Query: 243 TVTLCFVNKNSEEGATNLIHRWQYVAHDLDENIFLGILLAGGNF-TWQEGKINPTALFLS 302
           TVT+C   +N +     L+H+WQYVA+ LDEN+FLGI+L GGN  T QEG  NP A F S
Sbjct: 255 TVTMCNTYRNLKGDGIKLVHQWQYVANKLDENLFLGIILTGGNITTTQEGITNPIATFFS 314

Query: 303 LYLGKVEELVSILNTTFPELGLAKKDCTETSWVESTLIMGTGLQPGKALEDLLSKASLTN 362
           L+LGKV ELV+ L+TTFPELGL K+DC ETSW+ESTLI  TG+Q  ++LE LL++     
Sbjct: 315 LFLGKVNELVATLSTTFPELGLIKQDCIETSWIESTLIASTGVQTVESLEPLLNRTPSNL 374

Query: 363 ASTKIKSDYIKQPIPMAAVEGIWQRLKLEDIEMPQLLLSPYGGRMSQISESETPFSHRSE 422
            + KIKSDYIK+PI +A +EGIWQRLK +DIE  QL+  PYGGRMSQISESETPFSHR  
Sbjct: 375 ENEKIKSDYIKEPISIATIEGIWQRLKAQDIETSQLIFIPYGGRMSQISESETPFSHRVG 434

Query: 423 YLYTIGYVINWEQQGNDVEKRHLSWIRELYSYMAPFASKLPRAAYVNYRDLDIGSNYRYG 482
            LY IGY++ W++Q    +K+H+SWIRE+Y YM PF SK PR+AY NYRDLDIG N +YG
Sbjct: 435 NLYKIGYILGWKEQSLKAKKKHISWIREIYEYMTPFVSKSPRSAYANYRDLDIGVNKKYG 494

Query: 483 KTSYEKASIWGLKYFGNNFNRLVDVKTRFDPYDFFRHEQSIPTL 523
           KTS ++ASIWGLKYFGNNF RLV VKT+ DPYDFFRHEQSIPTL
Sbjct: 495 KTSVKQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPTL 537

BLAST of Lsi02G009770 vs. NCBI nr
Match: gi|778662000|ref|XP_011659198.1| (PREDICTED: cannabidiolic acid synthase-like 1 [Cucumis sativus])

HSP 1 Score: 770.4 bits (1988), Expect = 2.1e-219
Identity = 379/530 (71.51%), Postives = 437/530 (82.45%), Query Frame = 1

Query: 1   MSILLSSSSPWVASNTFERHENFLQCLSHHSPN---ISKVIYTPFNSSYSSILNFTLRNL 60
           ++I+LS+S  WV++    +H+NF+QCLS HS     ISKV+YTP NSSYSS+LNF++RNL
Sbjct: 14  LTIVLSASPSWVSAFP-HKHQNFVQCLSEHSSKSYPISKVVYTPINSSYSSVLNFSIRNL 73

Query: 61  RFSTPNTPKPLLIITPSHISHIQAAVICSKPYGLQIRTRSGGHDFEGLSYVAYLPFVILD 120
           RFS P TPKPLLIITPSH+SHIQAAVICSK + LQIRTRSGGHD+EGLSYVAY PF+I+D
Sbjct: 74  RFSKPETPKPLLIITPSHVSHIQAAVICSKSHDLQIRTRSGGHDYEGLSYVAYHPFIIVD 133

Query: 121 LINLSSISIDVETNTAWVESGATIGELYYRIAEKSRTLAFPAGTCPIVGVGGQFSGGGYG 180
           LINL SISID+  NTAWVESGAT+GELYYRI EKSRTLAFPAG CP VGVGG FSGGGYG
Sbjct: 134 LINLRSISIDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPTVGVGGHFSGGGYG 193

Query: 181 MLTRKYGLASDNVIDAYLVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPV 240
            L RKYGLA+DNVIDAYLVDANG  HDR+SMGEDLFWAIRGG GGSFGIVVAWKVKLVPV
Sbjct: 194 FLLRKYGLAADNVIDAYLVDANGMVHDRESMGEDLFWAIRGGSGGSFGIVVAWKVKLVPV 253

Query: 241 PATVTLCFVNKNSEEGATNLIHRWQYVAHDLDENIFLGILLAGGNFTWQEG----KINPT 300
           PA VT+C   KN E+ A  +IH+WQYVA+ L E+IFLGI+L GGN + Q G      NP 
Sbjct: 254 PAMVTICSTIKNLEDDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTSTQGGIKNPIKNPI 313

Query: 301 ALFLSLYLGKVEELVSILNTTFPELGLAKKDCTETSWVESTLIMGTGL-QPGKALEDLLS 360
           A F SL+LGKV+ELV+ L+T FPELGL K+DC E  WVESTLI+ +G+ Q  ++LE LL+
Sbjct: 314 ATFYSLFLGKVDELVATLSTKFPELGLIKQDCLEVRWVESTLIIASGVFQTIESLEPLLN 373

Query: 361 KASLTNASTKIKSDYIKQPIPMAAVEGIWQRLKLEDIEMPQLLLSPYGGRMSQISESETP 420
           +   T  STKIKSDYIK+PIP AA+EGIWQRLK  DIE PQ + +PYG RMSQIS+SETP
Sbjct: 374 RTPTTLDSTKIKSDYIKKPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARMSQISKSETP 433

Query: 421 FSHRSEYLYTIGYVINWEQQGNDVEKRHLSWIRELYSYMAPFASKLPRAAYVNYRDLDIG 480
           FSHR+ YLY IGY++ W+ Q    + RH+SWIRELY YMAPF SK PRAAY NYRDLDIG
Sbjct: 434 FSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAAYTNYRDLDIG 493

Query: 481 SNYRYGKTSYEKASIWGLKYFGNNFNRLVDVKTRFDPYDFFRHEQSIPTL 523
           SN +YGKTSY++ASIWGLKYFGNNF RLV VKT+ DP+DFFRHEQSIPTL
Sbjct: 494 SNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 542

BLAST of Lsi02G009770 vs. NCBI nr
Match: gi|659068690|ref|XP_008445695.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 769.6 bits (1986), Expect = 3.5e-219
Identity = 375/524 (71.56%), Postives = 430/524 (82.06%), Query Frame = 1

Query: 3   ILLSSSSPWVASNTFERHENFLQCLSHHSPN---ISKVIYTPFNSSYSSILNFTLRNLRF 62
           +++SSS  WV++   E H+NFL CL  HS     ISKV+YTP NSSYSS+LNF++RN RF
Sbjct: 17  VVVSSSPLWVSAFPHE-HQNFLHCLPEHSSKSYPISKVVYTPINSSYSSVLNFSIRNFRF 76

Query: 63  STPNTPKPLLIITPSHISHIQAAVICSKPYGLQIRTRSGGHDFEGLSYVAYLPFVILDLI 122
           S P TPKPLLIITPSH+SHIQAAVICSK +GLQIRTRSGGHD+EGLSYVAY PF+I+DLI
Sbjct: 77  SKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEGLSYVAYRPFIIVDLI 136

Query: 123 NLSSISIDVETNTAWVESGATIGELYYRIAEKSRTLAFPAGTCPIVGVGGQFSGGGYGML 182
           N+ SIS+D+  NTAWVESGAT+GELYYRI EKSRTLAFPAG CP VGVGG FSGGGYG L
Sbjct: 137 NIRSISVDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPTVGVGGHFSGGGYGFL 196

Query: 183 TRKYGLASDNVIDAYLVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPA 242
            RKYGLA+DNVIDAYLVDANG  HDR+SMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPA
Sbjct: 197 LRKYGLAADNVIDAYLVDANGMAHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPA 256

Query: 243 TVTLCFVNKNSEEGATNLIHRWQYVAHDLDENIFLGILLAGGNFTWQEGKINPTALFLSL 302
            VT+C   KN E  A  +IH+WQYVA+ L E+IFLGI+L GGN + Q G   PTA F SL
Sbjct: 257 LVTICSTIKNLEGDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTSTQGGIKKPTATFYSL 316

Query: 303 YLGKVEELVSILNTTFPELGLAKKDCTETSWVESTLIMGTGL-QPGKALEDLLSKASLTN 362
           +LGKV+ELV+ L+TTFPE GL KKDC E  W+ESTLI+ +G+ Q  ++L+ LL++   T 
Sbjct: 317 FLGKVDELVATLSTTFPEFGLIKKDCLEVRWIESTLIIASGVFQTVESLKPLLNRTPSTI 376

Query: 363 ASTKIKSDYIKQPIPMAAVEGIWQRLKLEDIEMPQLLLSPYGGRMSQISESETPFSHRSE 422
            STKIKSDYIK+PIP AA+EGIWQRLK  DIE PQL+  PYG RMSQISESETPFSHR+ 
Sbjct: 377 DSTKIKSDYIKKPIPKAAIEGIWQRLKAGDIEAPQLVFVPYGARMSQISESETPFSHRAG 436

Query: 423 YLYTIGYVINWEQQGNDVEKRHLSWIRELYSYMAPFASKLPRAAYVNYRDLDIGSNYRYG 482
           YLY IGY++ W+ Q    + RH+SW RELY YM PF SK PR AYVNYRDLDIGSN +YG
Sbjct: 437 YLYKIGYMVGWKGQNLKAKNRHISWTRELYEYMTPFVSKSPRTAYVNYRDLDIGSNNKYG 496

Query: 483 KTSYEKASIWGLKYFGNNFNRLVDVKTRFDPYDFFRHEQSIPTL 523
           KTSY++ASIWGLKYFGNNF RLV VK + DP+DFFRHEQSIPTL
Sbjct: 497 KTSYKQASIWGLKYFGNNFKRLVYVKNKVDPHDFFRHEQSIPTL 539

BLAST of Lsi02G009770 vs. NCBI nr
Match: gi|659068685|ref|XP_008445674.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 766.1 bits (1977), Expect = 3.9e-218
Identity = 367/524 (70.04%), Postives = 431/524 (82.25%), Query Frame = 1

Query: 3   ILLSSSSPWVASNTFERHENFLQCLSHHSPN---ISKVIYTPFNSSYSSILNFTLRNLRF 62
           IL+SSSS WV+++   +H+NFLQCLS HS     ISKV++TP NSSYSS+L+F++RNL F
Sbjct: 15  ILVSSSSSWVSASP-HKHQNFLQCLSEHSSKRYPISKVVHTPINSSYSSVLDFSIRNLLF 74

Query: 63  STPNTPKPLLIITPSHISHIQAAVICSKPYGLQIRTRSGGHDFEGLSYVAYLPFVILDLI 122
           S P TPKPLLIITPSH+SHIQAAVICSK +GLQIRTRSGGHDFEGLSYVAY PF+I+DLI
Sbjct: 75  SKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYHPFIIVDLI 134

Query: 123 NLSSISIDVETNTAWVESGATIGELYYRIAEKSRTLAFPAGTCPIVGVGGQFSGGGYGML 182
           NL SI IDVETNTAWVESGAT+GELYYRI EKSRTLAFP G CP VGVGG FSGGGYG++
Sbjct: 135 NLRSIKIDVETNTAWVESGATLGELYYRIGEKSRTLAFPGGVCPTVGVGGHFSGGGYGLM 194

Query: 183 TRKYGLASDNVIDAYLVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPA 242
            RK+GLA+DNVIDAYLVDANG+ HDRKSMGEDLFWAIRGGGGGSFGIVVAWK+KLV VPA
Sbjct: 195 LRKFGLAADNVIDAYLVDANGKVHDRKSMGEDLFWAIRGGGGGSFGIVVAWKIKLVSVPA 254

Query: 243 TVTLCFVNKNSEEGATNLIHRWQYVAHDLDENIFLGILLAGGNF-TWQEGKINPTALFLS 302
           TVT+C  N+N +     L+H+WQYVA+ LDEN+FLGI+L GGN  T QEG  NP A F S
Sbjct: 255 TVTICSTNRNLKGDGIKLVHQWQYVANKLDENLFLGIMLTGGNITTTQEGIANPIATFFS 314

Query: 303 LYLGKVEELVSILNTTFPELGLAKKDCTETSWVESTLIMGTGLQPGKALEDLLSKASLTN 362
           L+LGKV+ LV+ L+  FPELG  K+DC ETSW+ESTLI  TG+   ++LE LL++     
Sbjct: 315 LFLGKVDILVATLSAIFPELGFTKEDCVETSWIESTLIAATGVGTVESLEPLLNRTPSNL 374

Query: 363 ASTKIKSDYIKQPIPMAAVEGIWQRLKLEDIEMPQLLLSPYGGRMSQISESETPFSHRSE 422
            + K+KSDY K+PI +A +EG+WQRLK +DIE  QL+  PYGGRMSQISE ETPFSHR+ 
Sbjct: 375 ENGKMKSDYFKEPISIATIEGLWQRLKAKDIETSQLIFIPYGGRMSQISEMETPFSHRAG 434

Query: 423 YLYTIGYVINWEQQGNDVEKRHLSWIRELYSYMAPFASKLPRAAYVNYRDLDIGSNYRYG 482
            LY IGY + W++Q    +K+H+SWIR++Y YM PF SK PR+AY NYRDLDIG+N +YG
Sbjct: 435 ILYKIGYFVAWKEQSLKAKKKHISWIRDIYKYMTPFVSKSPRSAYANYRDLDIGANKKYG 494

Query: 483 KTSYEKASIWGLKYFGNNFNRLVDVKTRFDPYDFFRHEQSIPTL 523
           KTS  +ASIWGLKYFGNNF RLV VKT+ DPYDFFRHEQSIPTL
Sbjct: 495 KTSVRQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPTL 537

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
THCAS_CANSA1.5e-14850.88Tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=1 SV=1[more]
THCAI_CANSA1.8e-14649.51Inactive tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=3 SV=1[more]
CBDAS_CANSA6.7e-14650.87Cannabidiolic acid synthase OS=Cannabis sativa GN=CBDAS PE=1 SV=1[more]
CASL1_CANSA2.0e-14550.00Cannabidiolic acid synthase-like 1 OS=Cannabis sativa GN=CBDAS2 PE=2 SV=1[more]
CASL2_CANSA3.3e-14550.20Cannabidiolic acid synthase-like 2 OS=Cannabis sativa GN=CBDAS3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
B9NA97_POPTR3.2e-17959.81FAD-binding domain-containing family protein OS=Populus trichocarpa GN=POPTR_000... [more]
V4UIF2_9ROSI3.0e-17758.46Uncharacterized protein OS=Citrus clementina GN=CICLE_v10017782mg PE=4 SV=1[more]
A0A067GQ97_CITSI1.1e-17658.46Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g009115mg PE=4 SV=1[more]
V4W7E3_9ROSI2.6e-17659.61Uncharacterized protein OS=Citrus clementina GN=CICLE_v10018353mg PE=4 SV=1[more]
A0A059CBE6_EUCGR5.7e-17657.50Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D00070 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G20820.17.7e-14849.23 FAD-binding Berberine family protein[more]
AT5G44440.13.3e-14348.66 FAD-binding Berberine family protein[more]
AT5G44410.15.7e-14348.53 FAD-binding Berberine family protein[more]
AT1G30700.11.4e-14148.84 FAD-binding Berberine family protein[more]
AT2G34790.11.8e-14147.62 FAD-binding Berberine family protein[more]
Match NameE-valueIdentityDescription
gi|659068687|ref|XP_008445684.1|5.2e-22370.88PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo][more]
gi|778661997|ref|XP_004151192.2|7.1e-22070.99PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus][more]
gi|778662000|ref|XP_011659198.1|2.1e-21971.51PREDICTED: cannabidiolic acid synthase-like 1 [Cucumis sativus][more]
gi|659068690|ref|XP_008445695.1|3.5e-21971.56PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo][more]
gi|659068685|ref|XP_008445674.1|3.9e-21870.04PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO:0016614oxidoreductase activity, acting on CH-OH group of donors
GO:0050660flavin adenine dinucleotide binding
GO:0016491oxidoreductase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: INTERPRO
TermDefinition
IPR016169CO dehydrogenase flavoprotein-like, FAD-binding, subdomain 2
IPR016167FAD-bd_2_sub1
IPR016166FAD-bd_2
IPR012951BBE
IPR006094Oxid_FAD_bind_N
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006040 amino sugar metabolic process
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0008762 UDP-N-acetylmuramate dehydrogenase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G009770.1Lsi02G009770.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 67..203
score: 1.0
IPR012951Berberine/berberine-likePFAMPF08031BBEcoord: 462..520
score: 1.4
IPR016166FAD-binding, type 2PROFILEPS51387FAD_PCMHcoord: 63..237
score: 19
IPR016166FAD-binding, type 2unknownSSF56176FAD-binding/transporter-associated domain-likecoord: 18..238
score: 2.2
IPR016167FAD-binding, type 2, subdomain 1GENE3DG3DSA:3.30.43.10coord: 66..126
score: 1.
IPR016169CO dehydrogenase flavoprotein-like, FAD-binding, subdomain 2GENE3DG3DSA:3.30.465.10coord: 127..238
score: 1.8
NoneNo IPR availablePANTHERPTHR32448FAMILY NOT NAMEDcoord: 1..529
score:

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Lsi02G009770CsGy1G024620Cucumber (Gy14) v2cgyblsiB019
The following gene(s) are paralogous to this gene:

None