BLAST of Lsi02G009700 vs. Swiss-Prot
Match:
THCAS_CANSA (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=1 SV=1)
HSP 1 Score: 344.7 bits (883), Expect = 1.4e-93
Identity = 203/461 (44.03%), Postives = 275/461 (59.65%), Query Frame = 1
Query: 3 FLAFIIVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFN--LIYTPTNSSFSHIL 62
F+ II L+F I S AN ++ FL+C S H P +++ N L+YT + + IL
Sbjct: 10 FVCKIIFFFLSFHIQISIANPRE--NFLKCFSKHIP---NNVANPKLVYTQHDQLYMSIL 69
Query: 63 NFYIRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSN 122
N I+NLRF + TTPKPL+I+TP H+QA ++C K G QIR RSGGHD EG+SY+S
Sbjct: 70 NSTIQNLRFISDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQ 129
Query: 123 VP-----------------------------------IAEKSKTLAFPAGGCPTVGVGGH 182
VP I EK++ L+FP G CPTVGVGGH
Sbjct: 130 VPFVVVDLRNMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGH 189
Query: 183 LSGGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAW 242
SGGG+G L+R YGLAAD++IDA LV+ +GK+LDRKSMGEDLFWAIRGGGG +FGI+ AW
Sbjct: 190 FSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAW 249
Query: 243 KIKLLYVPPTVTVFTVNKKLD-QNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLKKG-GI 302
KIKL+ VP T+F+V K ++ +KL ++WQ +A K D +L F N+ G
Sbjct: 250 KIKLVAVPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGK 309
Query: 303 RRTRTRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAG---FT 362
+T TV+ F S+F G + L+ +M KSFPELG+ K DC E +WI++ + +G F
Sbjct: 310 NKT---TVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFN 369
Query: 363 NGESLEEALLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPY 420
+E LL+RS A +K DYVK+PI ET + + + L ++ + VL PY
Sbjct: 370 TANFKKEILLDRSAGKK--TAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPY 429
BLAST of Lsi02G009700 vs. Swiss-Prot
Match:
THCAI_CANSA (Inactive tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=3 SV=1)
HSP 1 Score: 344.4 bits (882), Expect = 1.8e-93
Identity = 201/459 (43.79%), Postives = 271/459 (59.04%), Query Frame = 1
Query: 3 FLAFIIVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILNF 62
F+ II L+F I S AN Q+ FL+C S + P + IYT + + +LN
Sbjct: 10 FVCKIIFFFLSFNIQISIANPQE--NFLKCFSEYIPNNPANP-KFIYTQHDQLYMSVLNS 69
Query: 63 YIRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNVP 122
I+NLRF++ TTPKPL+I+TP H+QA ++C K G QIR RSGGHD EGLSY+S VP
Sbjct: 70 TIQNLRFTSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVP 129
Query: 123 IA-----------------------------------EKSKTLAFPAGGCPTVGVGGHLS 182
A E ++ +FP G CPTVGVGGH S
Sbjct: 130 FAIVDLRNMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFS 189
Query: 183 GGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKI 242
GGG+G L+R YGLAAD++IDA LV+ +GK+LDRKSMGEDLFWAIRGGGG +FGI+ AWKI
Sbjct: 190 GGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKI 249
Query: 243 KLLYVPPTVTVFTVNKKLD-QNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLKKG-GIRR 302
KL+ VP T+F+V K ++ +KL ++WQ +A K D +L FR N+ G +
Sbjct: 250 KLVVVPSKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNK 309
Query: 303 TRTRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESL 362
T TV+ F S+FLG + L+ +M KSFPELG+ K DC E +WI++ + +G N +
Sbjct: 310 T---TVHGYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTA 369
Query: 363 ---EEALLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGG 420
+E LL+RS A +K DYVK+ I ET + + + L + VL PYGG
Sbjct: 370 NFKKEILLDRSAGKK--TAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGG 429
BLAST of Lsi02G009700 vs. Swiss-Prot
Match:
CASL1_CANSA (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa GN=CBDAS2 PE=2 SV=1)
HSP 1 Score: 340.1 bits (871), Expect = 3.4e-92
Identity = 198/459 (43.14%), Postives = 274/459 (59.69%), Query Frame = 1
Query: 3 FLAFIIVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILNF 62
++ II L+F I S AN Q+ FL+C S + P + L+YT + + ILN
Sbjct: 10 YVCKIIFFFLSFNIQISIANPQE--NFLKCFSQYIPTNVTNA-KLVYTQHDQFYMSILNS 69
Query: 63 YIRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNVP 122
I+NLRF++ TTPKPL+IITPL H+Q ++C K G QIR RSGGHD EG+SY+S VP
Sbjct: 70 TIQNLRFTSDTTPKPLVIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVP 129
Query: 123 -----------------------------------IAEKSKTLAFPAGGCPTVGVGGHLS 182
I E ++ L+FPAG CPTVG GGH S
Sbjct: 130 FVIVDLRNMHSVKIDVHSQTAWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFS 189
Query: 183 GGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKI 242
GGG+G L+R YGLAAD++IDA LV+ +GK+LDRKSMGEDLFWAIRGGGG +FGI+ AWKI
Sbjct: 190 GGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKI 249
Query: 243 KLLYVPPTVTVFTVNKKLDQNTL-KLIHQWQYVASKLDDNLGGKDFFRPNNLKKG-GIRR 302
+L+ VP T+F+V K ++ + L KL+++WQ +A + L F N+ G +
Sbjct: 250 RLVAVPSMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNK 309
Query: 303 TRTRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESL 362
T T+++ F S+F G + L+ +M KSFPELG+ K DC + +WI++I+ +G N +
Sbjct: 310 T---TIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTT 369
Query: 363 ---EEALLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGG 420
+E LL+RS A +K DYVK+PI ET + + + L ++ V PYGG
Sbjct: 370 YFKKEILLDRSGGRK--AAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGG 429
BLAST of Lsi02G009700 vs. Swiss-Prot
Match:
CASL2_CANSA (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa GN=CBDAS3 PE=2 SV=1)
HSP 1 Score: 339.0 bits (868), Expect = 7.6e-92
Identity = 197/459 (42.92%), Postives = 273/459 (59.48%), Query Frame = 1
Query: 3 FLAFIIVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILNF 62
++ II L+F I S AN Q+ FL+CLS + P + L+YT + + ILN
Sbjct: 10 YVCKIIFFFLSFNIQISIANPQE--NFLKCLSQYIPTNVTNA-KLVYTQHDQFYMSILNS 69
Query: 63 YIRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNVP 122
++NLRF++ TTPKPL+I TPL H+Q ++C K G QIR RSGGHD EG+SY+S VP
Sbjct: 70 TVQNLRFTSDTTPKPLVITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVP 129
Query: 123 -----------------------------------IAEKSKTLAFPAGGCPTVGVGGHLS 182
I E ++ L+FPAG CPTVG GGH S
Sbjct: 130 FVIVDLRNMHSVKIDVHSQTAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFS 189
Query: 183 GGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKI 242
GGG+G L+R YGLAAD++IDA LV+ +GK+LDRKSMGEDLFWAIRGGGG +FGI+ AWKI
Sbjct: 190 GGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKI 249
Query: 243 KLLYVPPTVTVFTVNKKLDQNTL-KLIHQWQYVASKLDDNLGGKDFFRPNNLKKG-GIRR 302
+L+ VP T+F+V K ++ + L KL+++WQ +A + L F N+ G +
Sbjct: 250 RLVAVPSMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNK 309
Query: 303 TRTRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESL 362
T T+++ F S+F G + L+ +M KSFPELG+ K DC + +WI++I+ +G N +
Sbjct: 310 T---TIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTT 369
Query: 363 ---EEALLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGG 420
+E LL+RS A +K DYVK+PI ET + + + L ++ V PYGG
Sbjct: 370 NFKKEILLDRSGGRK--AAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGG 429
BLAST of Lsi02G009700 vs. Swiss-Prot
Match:
RETOL_ARATH (Reticuline oxidase-like protein OS=Arabidopsis thaliana GN=At4g20830 PE=1 SV=2)
HSP 1 Score: 336.7 bits (862), Expect = 3.8e-91
Identity = 188/454 (41.41%), Postives = 268/454 (59.03%), Query Frame = 1
Query: 3 FLAFIIVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILNF 62
FL F+ + + +F +S++ Y FL+C S + I + +++ TN +FS +L
Sbjct: 18 FLLFLSLPLSSFS--QSNSVYNS---FLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRA 77
Query: 63 YIRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNVP 122
YIRN RF+T++T KP +IITP HV A V C K F ++IRSGGHDY+GLSY+S+ P
Sbjct: 78 YIRNARFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISDKP 137
Query: 123 -----------------------------------IAEKSKTLAFPAGGCPTVGVGGHLS 182
I EKS+ FPAG CPTVGVGGHLS
Sbjct: 138 FFILDMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLS 197
Query: 183 GGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKI 242
GGG+G ++RK+GL+ D+V DA +VD NG++LDRK+MGEDLFWAI GGGGGS+G+VL +K+
Sbjct: 198 GGGYGNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKV 257
Query: 243 KLLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLKKGGIRRTR 302
KL+ VP VTVF V + +D + ++H+WQ V K D NL + +P + R +
Sbjct: 258 KLVPVPSVVTVFRVEQYMDSGAVDMVHKWQSVGPKTDPNLFMRMLIQP-------VTRKK 317
Query: 303 TRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESLE- 362
+TV AS +LFLG+A+E+++++ K FPELGL KE+C E W +S L N ++
Sbjct: 318 VKTVRASVVALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDP 377
Query: 363 EALLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMNE 421
+ L+R+ + S G K KSDYV I + G++ L+K + + +V PYGGKM E
Sbjct: 378 KVFLDRNLDTSSFG--KRKSDYVATAIPKKGIESLFK--KMIELGKIGLVFNPYGGKMAE 437
BLAST of Lsi02G009700 vs. TrEMBL
Match:
B9SAY8_RICCO (Reticuline oxidase, putative OS=Ricinus communis GN=RCOM_1334770 PE=4 SV=1)
HSP 1 Score: 435.6 bits (1119), Expect = 6.7e-119
Identity = 233/447 (52.13%), Postives = 304/447 (68.01%), Query Frame = 1
Query: 4 LAFIIVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILNFY 63
L ++ S+L+F W +SA + +FL+CL L S + SI +IYTP NSS+S +L F
Sbjct: 8 LPVLVFSLLSFS-WATSAQTHE--DFLECLHLQS-QDSTSISRIIYTPINSSYSSVLQFS 67
Query: 64 IRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNVP- 123
I+N RF+T+TTPKPL+I+TPL HVQA +IC K HG IR+RSGGHDYEGLSYVS P
Sbjct: 68 IQNRRFNTSTTPKPLVIVTPLNVSHVQAAIICSKRHGMHIRVRSGGHDYEGLSYVSVHPF 127
Query: 124 ----------------------------------IAEKSKTLAFPAGGCPTVGVGGHLSG 183
IAE+S+TLAFPAG CPTVG+GGH +G
Sbjct: 128 VIVDLINLQSITVDAANNTAWVQAGATIGNLYYSIAERSRTLAFPAGVCPTVGIGGHFTG 187
Query: 184 GGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKIK 243
GG+G LLRKYGLAAD++IDA+L+D NG++LDR SMGEDLFWAIRGGGG +FGIV++WKI
Sbjct: 188 GGYGMLLRKYGLAADNIIDAVLIDVNGRVLDRASMGEDLFWAIRGGGGNTFGIVISWKIN 247
Query: 244 LLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLKKGGIRRTRT 303
L+ VP TVTVFTV K L+QN +L+++WQY+A KL ++L F R + +
Sbjct: 248 LVPVPATVTVFTVEKTLEQNATQLVNRWQYIADKLHEDL----FIRVIIERVNSTSQQGK 307
Query: 304 RTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESLEEA 363
TV A+F SLFLG + L+ +MQ+SFPELGL++EDCIE +WIESIL AGF+N S +
Sbjct: 308 TTVRAAFNSLFLGGVDRLLPLMQESFPELGLVREDCIEMSWIESILYFAGFSN--SPLDI 367
Query: 364 LLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMNEIS 416
LLNR T PS + K KSDYVKEP+ ET L+G+W+ L ++ + ++ PYGG+M+EIS
Sbjct: 368 LLNR-TQPS-VRNFKAKSDYVKEPMPETALEGIWERLSEVDVGAGQLIFSPYGGRMSEIS 427
BLAST of Lsi02G009700 vs. TrEMBL
Match:
B9NA97_POPTR (FAD-binding domain-containing family protein OS=Populus trichocarpa GN=POPTR_0001s43120g PE=4 SV=1)
HSP 1 Score: 433.0 bits (1112), Expect = 4.3e-118
Identity = 234/453 (51.66%), Postives = 298/453 (65.78%), Query Frame = 1
Query: 1 MNFLAFIIVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHIL 60
++FL F+I S W +SA+ + FLQCL + SI LIYTP NSS+S IL
Sbjct: 2 LSFLFFLIFSFS----WVTSAHTHE--SFLQCLD---SQNSHSISKLIYTPINSSYSSIL 61
Query: 61 NFYIRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSN 120
F I+NLRF+T++TPKPL+I+TP H+QA +IC + HG QIRIRSGGHDYEGLSYVS
Sbjct: 62 QFSIQNLRFNTSSTPKPLVIVTPTNISHIQAAIICSQKHGMQIRIRSGGHDYEGLSYVST 121
Query: 121 VP-----------------------------------IAEKSKTLAFPAGGCPTVGVGGH 180
IAEKS+TLAFPA CPTVGVGGH
Sbjct: 122 FSFVVIDLINLRTINVNAENKTAWVQSGVTIGEVYYRIAEKSRTLAFPASVCPTVGVGGH 181
Query: 181 LSGGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAW 240
SGGG+G L+RKYGLAADH+IDA LVD G++LDR+SMGEDLFWAIRGGGG +FG+V+AW
Sbjct: 182 FSGGGYGMLMRKYGLAADHIIDAQLVDVKGRILDRESMGEDLFWAIRGGGGNTFGVVIAW 241
Query: 241 KIKLLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLKKGGIRR 300
KI L+ PPTVTVF V + L+QN KL+H+WQ+VA KL ++L + N + G
Sbjct: 242 KISLVSAPPTVTVFNVQRTLEQNASKLVHRWQFVADKLHEDLFIRIILNRVNSTEEGKSN 301
Query: 301 TRTRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESL 360
T T+ ASF SLFLG + L+ ++Q SFPELGL+KEDCIE +WI+S+L GF + SL
Sbjct: 302 T---TIQASFNSLFLGGIDRLLPLIQDSFPELGLVKEDCIEMSWIQSVLYFDGFPSNSSL 361
Query: 361 EEALLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMN 419
+ LL+R+ PS K KSDYVKEPI E GL+G+W+ +I + ++ PYGGKM+
Sbjct: 362 -DVLLDRT--PSTRRNFKAKSDYVKEPIPELGLEGIWERFFDKDINTPILIFSPYGGKMS 421
BLAST of Lsi02G009700 vs. TrEMBL
Match:
U5FWX5_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0011s16570g PE=4 SV=1)
HSP 1 Score: 426.0 bits (1094), Expect = 5.3e-116
Identity = 230/448 (51.34%), Postives = 303/448 (67.63%), Query Frame = 1
Query: 2 NFLAFIIVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILN 61
+ L F+++ + F W +SA+ + +FLQCLSL+S + + +IYTP N+S++ +L
Sbjct: 7 SMLPFLLILLFPFS-WGTSAHTHE--DFLQCLSLYS-EDSAATSKVIYTPNNTSYATVLQ 66
Query: 62 FYIRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNV 121
F IRNLRF+++ P++I+TP H+QA + C + H QIRIRSGGHDYEGLSY+S +
Sbjct: 67 FSIRNLRFNSSKLT-PVVIVTPTILSHIQATIHCSQKHNLQIRIRSGGHDYEGLSYMSVL 126
Query: 122 P-----------------------------------IAEKSKTLAFPAGGCPTVGVGGHL 181
P IAEKS+TLAFPAG C TVGVGG
Sbjct: 127 PFVILDLINLRKITVDLSTKTAWVQAGATLGELYYSIAEKSRTLAFPAGACHTVGVGGQF 186
Query: 182 SGGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWK 241
SGGG+GGLLRKYGLAAD+VIDA L+DANG++LDR+SMGEDLFWAIRGGGG SFG+V AWK
Sbjct: 187 SGGGYGGLLRKYGLAADNVIDAELIDANGRVLDRESMGEDLFWAIRGGGGNSFGVVTAWK 246
Query: 242 IKLLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLKKGGIRRT 301
+ L+ VPPTVTVF V K L +N KLIH+WQYVA+KL +++ + N +GG
Sbjct: 247 VNLVEVPPTVTVFAVPKVLKENATKLIHRWQYVANKLPEDIVIAAYVNRINSSQGG---- 306
Query: 302 RTRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESLE 361
T+ A+F SLFLG + L+ +MQ+SFPELGL+K+DCIE +WIE L LAGF + SL
Sbjct: 307 -NPTIQATFTSLFLGGVDRLLPLMQESFPELGLVKDDCIELSWIEFALFLAGFPSNASL- 366
Query: 362 EALLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMNE 415
+ LL+R+ P I + K KSDYVK+P+ ET L+G+W+ +IES S+ ++PYGGKM E
Sbjct: 367 DVLLDRT--PQSITSFKAKSDYVKQPLPETALEGMWETFFEKDIESPSLFMVPYGGKMEE 426
BLAST of Lsi02G009700 vs. TrEMBL
Match:
A0A061EKB4_THECC (FAD-binding Berberine family protein OS=Theobroma cacao GN=TCM_020191 PE=4 SV=1)
HSP 1 Score: 423.7 bits (1088), Expect = 2.6e-115
Identity = 232/442 (52.49%), Postives = 291/442 (65.84%), Query Frame = 1
Query: 3 FLAFIIVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILNF 62
FL ++VS+ W SAN + +FL CLS K I +IYTP NSS+S +L
Sbjct: 4 FLLLVLVSLS----WAISANAHEN-DFLHCLSSFHSKDTSPISKVIYTPNNSSYSSVLES 63
Query: 63 YIRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNVP 122
I+NLRFS TTPKPL+IITP H+QA + C + HGFQIRIRSGGHD+EGLSYVS+VP
Sbjct: 64 SIQNLRFSEPTTPKPLIIITPKHTSHIQASIHCSRKHGFQIRIRSGGHDFEGLSYVSHVP 123
Query: 123 -----------------------------------IAEKSKTLAFPAGGCPTVGVGGHLS 182
IA+KS+TLAFPAG C TVG+GG+ S
Sbjct: 124 FIIVDLVNFRSVDIDDKNKVAWIQSGAILGEFYYGIAQKSRTLAFPAGVCHTVGIGGYFS 183
Query: 183 GGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKI 242
GGG+G L RKYGLA D++IDAL +D NG++LDR+SMGEDLFWAIRGGGGGSFGIVLAWK+
Sbjct: 184 GGGYGLLFRKYGLAVDNIIDALFIDVNGRILDRESMGEDLFWAIRGGGGGSFGIVLAWKV 243
Query: 243 KLLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLKKGGIRRTR 302
KL+ VP TVTVF +++ L+QN KL+H+WQYVA KL D D F +++ +
Sbjct: 244 KLVSVPATVTVFRISRTLEQNATKLVHRWQYVAHKLPD-----DMFSAVTMRRVNSSQDG 303
Query: 303 TRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESLEE 362
RT+ ASF S FLG +ELI +MQ+ FPELGL+KEDC E +WIESIL N +SL +
Sbjct: 304 KRTILASFSSFFLGGIDELIPLMQERFPELGLVKEDCTEMSWIESILYFGQIQN-KSL-D 363
Query: 363 ALLNRSTNPSFIGAN-KVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMNE 409
LL+RS + G + K KSDYVK+PI ET L+ +W L + ESA I + YGGKM+E
Sbjct: 364 ILLDRSYKTTLNGPSFKTKSDYVKKPIPETALEEIWSKLYEEDAESAVIGFIAYGGKMDE 423
BLAST of Lsi02G009700 vs. TrEMBL
Match:
V7CLG0_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_002G199800g PE=4 SV=1)
HSP 1 Score: 420.2 bits (1079), Expect = 2.9e-114
Identity = 228/448 (50.89%), Postives = 304/448 (67.86%), Query Frame = 1
Query: 3 FLAFIIVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILNF 62
F+A +++L F + SSA+ + +F+QCL + P SI N++YT TNSS+S +L+
Sbjct: 8 FIAVTAIALL-FSLTPSSADSHE--KFVQCL-YNYPHNTTSISNVVYTQTNSSYSSVLDV 67
Query: 63 YIRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNVP 122
I+NLRF AT+ KPL+++TPL H+QA +IC + HG QIR RSGGHDYEGLSYV+ VP
Sbjct: 68 SIQNLRFFNATS-KPLVVVTPLTVSHIQATIICSQRHGLQIRTRSGGHDYEGLSYVAGVP 127
Query: 123 -----------------------------------IAEKSKTLAFPAGGCPTVGVGGHLS 182
I++KS+ L FPAG CP VGVGGH S
Sbjct: 128 FVVVDLIKLRGIVVDVENSTAWVEAGATIGELYYRISQKSEILGFPAGVCPPVGVGGHFS 187
Query: 183 GGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKI 242
GGG+G L+RK+GLAAD+VIDA +VD NG LLD+++MGEDLFWAIRGGGG SFG+++AWKI
Sbjct: 188 GGGYGFLMRKFGLAADNVIDAHIVDVNGNLLDKETMGEDLFWAIRGGGGASFGVIVAWKI 247
Query: 243 KLLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGK-DFFRPNNLKKGGIRRT 302
KL+ VP TVTVF V + L++N ++IH+WQ VA+KL+++L + + R N+ + G +
Sbjct: 248 KLVPVPSTVTVFRVPRTLEENATEIIHKWQRVANKLNESLTIRINMGRVNSSQNGNL--- 307
Query: 303 RTRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESLE 362
TV A F SL+LG+ ++LI +MQ SFPELGL++EDC E +WIESIL +AGFTNGES
Sbjct: 308 ---TVQAQFESLYLGRVDDLIPLMQNSFPELGLVREDCTEMSWIESILYMAGFTNGES-T 367
Query: 363 EALLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMNE 415
+ LLNR T + + K KSDYVK+PI + GL+GLW L E A + PYGG+MNE
Sbjct: 368 DVLLNR-TQLNGLSFFKAKSDYVKDPIPDVGLQGLWPFLYEDEAEDAFVQFTPYGGRMNE 427
BLAST of Lsi02G009700 vs. TAIR10
Match:
AT1G30700.1 (AT1G30700.1 FAD-binding Berberine family protein)
HSP 1 Score: 358.6 bits (919), Expect = 5.2e-99
Identity = 201/441 (45.58%), Postives = 271/441 (61.45%), Query Frame = 1
Query: 5 AFIIVSILTFGIWESSANYQKPIEFLQCLSLHS-PKGDDSIFNLIYTPTNSSFSHILNFY 64
A I+V IW+SS++ F QCL+ +S PK I I+ N S+S +L
Sbjct: 4 ALILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPK--HPISPAIFFSGNGSYSSVLQAN 63
Query: 65 IRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVS---- 124
IRNLRF+T +TPKP LII HVQA + C K H Q++IRSGGHDY+GLSYV+
Sbjct: 64 IRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGK 123
Query: 125 -------------NVPIA--------------------EKSKTLAFPAGGCPTVGVGGHL 184
+V +A EKSKTLA+PAG CPTVGVGGH+
Sbjct: 124 PFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHI 183
Query: 185 SGGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWK 244
SGGG+G ++RKYGL D+ IDA +VD NGK+LDRK MGEDL+WAI GGGGGS+G+VLA+K
Sbjct: 184 SGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYK 243
Query: 245 IKLLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFRP-NNLKKGGIRR 304
I L+ VP VTVF +++ L+QN +IH+WQ VA KL D L F R ++ G +
Sbjct: 244 INLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDEL----FIRTVIDVVNGTV-- 303
Query: 305 TRTRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESL 364
+ +TV +F ++FLG L+SI+ + FPELGL++ DC E +WI+S+L G S
Sbjct: 304 SSQKTVRTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSS- 363
Query: 365 EEALLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMN 407
E LL R+ +++ K KSDYV+EPIS TGL+ +WK + +E ++ PYGG+M
Sbjct: 364 ETLLLQRNQPVNYL---KRKSDYVREPISRTGLESIWK--KMIELEIPTMAFNPYGGEMG 423
BLAST of Lsi02G009700 vs. TAIR10
Match:
AT4G20840.1 (AT4G20840.1 FAD-binding Berberine family protein)
HSP 1 Score: 342.4 bits (877), Expect = 3.9e-94
Identity = 191/454 (42.07%), Postives = 266/454 (58.59%), Query Frame = 1
Query: 3 FLAFIIVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILNF 62
F F +VS+ F ++ F+QC S + I + +++ TN SFS +L
Sbjct: 12 FFVFFLVSLPFFSSAAPPSSDSIYESFVQCFSDKTKSPQAQITDNVFSRTNPSFSSVLRA 71
Query: 63 YIRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNVP 122
YIRN RF+T++TPKP +I+TP HV A V C K F ++IRSGGHDYEGLSY+S+ P
Sbjct: 72 YIRNGRFNTSSTPKPAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLSYISDKP 131
Query: 123 -----------------------------------IAEKSKTLAFPAGGCPTVGVGGHLS 182
I EKSK FPAG CPTVGVGGH+S
Sbjct: 132 FFILDMSNLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVGVGGHIS 191
Query: 183 GGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKI 242
GGG+G +LRK+GL+ D++IDA +VD NG++LDRKSMGEDLFWAI GGGG SFG+VL +K+
Sbjct: 192 GGGYGNMLRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGVVLGYKV 251
Query: 243 KLLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLKKGGIRRTR 302
KL+ VP TVTVF V K +D + ++H+WQ V K D NL + +P + R +
Sbjct: 252 KLVPVPETVTVFRVEKYMDSGAVDMVHKWQSVGPKTDRNLFLRMLIQP-------VTRKK 311
Query: 303 TRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESLE- 362
+TV A+ +LFLG+A E+++++ K FPEL L KE+C E W +S L N ++
Sbjct: 312 VKTVRATVVALFLGRAEEVVALLGKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQIDP 371
Query: 363 EALLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMNE 421
+ L+R+ + + G K KSDYV I G++ L+K + T + +V PYGGKM E
Sbjct: 372 KVFLDRNLDRANFG--KRKSDYVASEIPRDGIESLFK--KMTELGKIGLVFNPYGGKMAE 431
BLAST of Lsi02G009700 vs. TAIR10
Match:
AT4G20820.1 (AT4G20820.1 FAD-binding Berberine family protein)
HSP 1 Score: 340.5 bits (872), Expect = 1.5e-93
Identity = 197/441 (44.67%), Postives = 261/441 (59.18%), Query Frame = 1
Query: 4 LAFIIVSILTFGIWES-SANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILNF 63
L F S L I SAN FLQCLSL + + +I+TP ++SFS +L
Sbjct: 11 LIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQL-NDSNIVSKVIHTPNDTSFSSVLAS 70
Query: 64 YIRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNVP 123
I+N RFS PKP+LI+TP+QP VQ+ V C + G IR RSGGHDYEGLSYV++ P
Sbjct: 71 SIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKP 130
Query: 124 IA------EKSKTLAFP-----------------------------AGGCPTVGVGGHLS 183
+S T+ AG CPTVGVGGH S
Sbjct: 131 FVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFS 190
Query: 184 GGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKI 243
GGG+G LLRK+GLAADHVIDA +VDA G++L+R+ MGED FWAIRGGGG SF +VL+WKI
Sbjct: 191 GGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKI 250
Query: 244 KLLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLKKGGIRRTR 303
L+ VP TVTVF V K +Q+ LK+IH+WQ+VA K+ D+L F R + +
Sbjct: 251 GLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDL----FIRV-------MLQRY 310
Query: 304 TRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESLEE 363
V ASF L+LG L+ ++ K FPELGL ++DC E +WIES++ A E EE
Sbjct: 311 KNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFA-----ELGEE 370
Query: 364 AL--LNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMN 407
+ L + T S A K KSD+V+EP+ +T + LW+ L+ E A ++ P+GGKM+
Sbjct: 371 PINVLTKRTRASL--AFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMS 430
BLAST of Lsi02G009700 vs. TAIR10
Match:
AT1G01980.1 (AT1G01980.1 FAD-binding Berberine family protein)
HSP 1 Score: 337.8 bits (865), Expect = 9.6e-93
Identity = 192/456 (42.11%), Postives = 273/456 (59.87%), Query Frame = 1
Query: 1 MNFLAFIIVS-ILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHI 60
++ L F+IVS +++ + + N FLQC S + + + +++ +++SF+
Sbjct: 3 LSCLVFLIVSSLVSSSLATAPPNTSIYESFLQCFSNQTGAPPEKLCDVVLPQSSASFTPT 62
Query: 61 LNFYIRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVS 120
L YIRN RF+T+T+PKPLL+I HVQA V+C K FQ++ RSGGHDY+G+SY+S
Sbjct: 63 LRAYIRNARFNTSTSPKPLLVIAARSECHVQATVLCTKSLNFQLKTRSGGHDYDGVSYIS 122
Query: 121 NVP-------------------------------------IAEKSKT---LAFPAGGCPT 180
N P I + SKT FPAG CPT
Sbjct: 123 NRPFFVLDMSYLRNITVDMSDDGGSAWVGAGATLGEVYYNIWQSSKTHGTHGFPAGVCPT 182
Query: 181 VGVGGHLSGGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSF 240
VG GGH+SGGG+G ++RKYGL+ D+V DA +VD NG++LDRKSMGEDLFWAI GGGG SF
Sbjct: 183 VGAGGHISGGGYGNMIRKYGLSVDYVTDAKIVDVNGRILDRKSMGEDLFWAIGGGGGASF 242
Query: 241 GIVLAWKIKLLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLK 300
G++L++KIKL+ VPP VTVF V K L +N L ++H+WQ+VA K +L + +P
Sbjct: 243 GVILSFKIKLVPVPPRVTVFRVEKTLVENALDMVHKWQFVAPKTSPDLFMRLMLQP---- 302
Query: 301 KGGIRRTRTRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGF 360
+ R T+TV AS +LFLG+ ++L+S++ K FPELGL E+C E WI+S++ A
Sbjct: 303 ---VTRNTTQTVRASVVALFLGKQSDLMSLLTKEFPELGLKPENCTEMTWIQSVMWWANN 362
Query: 361 TNGESLE-EALLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLL 415
N ++ E LL+R NP K KSDYV++ IS+ GL L K L +V
Sbjct: 363 DNATVIKPEILLDR--NPDSASFLKRKSDYVEKEISKDGLDFLCKKLMEAG--KLGLVFN 422
BLAST of Lsi02G009700 vs. TAIR10
Match:
AT4G20830.1 (AT4G20830.1 FAD-binding Berberine family protein)
HSP 1 Score: 336.7 bits (862), Expect = 2.1e-92
Identity = 188/454 (41.41%), Postives = 268/454 (59.03%), Query Frame = 1
Query: 3 FLAFIIVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILNF 62
FL F+ + + +F +S++ Y FL+C S + I + +++ TN +FS +L
Sbjct: 18 FLLFLSLPLSSFS--QSNSVYNS---FLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRA 77
Query: 63 YIRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNVP 122
YIRN RF+T++T KP +IITP HV A V C K F ++IRSGGHDY+GLSY+S+ P
Sbjct: 78 YIRNARFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISDKP 137
Query: 123 -----------------------------------IAEKSKTLAFPAGGCPTVGVGGHLS 182
I EKS+ FPAG CPTVGVGGHLS
Sbjct: 138 FFILDMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLS 197
Query: 183 GGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKI 242
GGG+G ++RK+GL+ D+V DA +VD NG++LDRK+MGEDLFWAI GGGGGS+G+VL +K+
Sbjct: 198 GGGYGNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKV 257
Query: 243 KLLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLKKGGIRRTR 302
KL+ VP VTVF V + +D + ++H+WQ V K D NL + +P + R +
Sbjct: 258 KLVPVPSVVTVFRVEQYMDSGAVDMVHKWQSVGPKTDPNLFMRMLIQP-------VTRKK 317
Query: 303 TRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESLE- 362
+TV AS +LFLG+A+E+++++ K FPELGL KE+C E W +S L N ++
Sbjct: 318 VKTVRASVVALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDP 377
Query: 363 EALLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMNE 421
+ L+R+ + S G K KSDYV I + G++ L+K + + +V PYGGKM E
Sbjct: 378 KVFLDRNLDTSSFG--KRKSDYVATAIPKKGIESLFK--KMIELGKIGLVFNPYGGKMAE 437
BLAST of Lsi02G009700 vs. NCBI nr
Match:
gi|1009107976|ref|XP_015882457.1| (PREDICTED: cannabidiolic acid synthase-like 2, partial [Ziziphus jujuba])
HSP 1 Score: 446.0 bits (1146), Expect = 7.1e-122
Identity = 236/425 (55.53%), Postives = 290/425 (68.24%), Query Frame = 1
Query: 19 SSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILNFYIRNLRFSTATTPKPL 78
SSA+ FLQCLS H P +I LIYT TNSS++ +LNF IRN RFS+++TPKPL
Sbjct: 24 SSASAHTHENFLQCLS-HYPNTTSNISQLIYTQTNSSYAPVLNFSIRNPRFSSSSTPKPL 83
Query: 79 LIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNVP---------------- 138
+IITP+Q H+QA + C + HG Q+RIRSGGHDYEGLSYVS VP
Sbjct: 84 VIITPVQLSHIQAAINCSRKHGMQVRIRSGGHDYEGLSYVSQVPFVVIDLINLRSITVDV 143
Query: 139 -------------------IAEKSKTLAFPAGGCPTVGVGGHLSGGGHGGLLRKYGLAAD 198
IAEKS+TL FPAG CPTVGVGGH GGG+G +LRKYGLAAD
Sbjct: 144 DERTAWIESGATIGEVYYRIAEKSRTLGFPAGVCPTVGVGGHFGGGGYGTMLRKYGLAAD 203
Query: 199 HVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKIKLLYVPPTVTVFTVNK 258
VIDA +VD NG+ LDR+SMGEDLFWAIRGGGG SFG+VLAWK+KL+ VP TVTVF+VN+
Sbjct: 204 KVIDAHIVDVNGRFLDRQSMGEDLFWAIRGGGGASFGVVLAWKVKLVPVPSTVTVFSVNR 263
Query: 259 KLDQNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLKKGGIRRTRTRTVNASFFSLFLGQA 318
L+QN +L+H+WQYVA K D++L + + N G RT+ ASF SLFLG
Sbjct: 264 NLEQNVTQLVHRWQYVADKFDEDLFVRIILQSLNASGEG-----KRTIQASFNSLFLGGV 323
Query: 319 NELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESLEEALLNRSTNPSFIGANK 378
+ LI +M KSFPELGL KEDC E +WIES L GF++GESL + LL+R+ PS + K
Sbjct: 324 DRLIPLMDKSFPELGLTKEDCTEMSWIESTLYFNGFSSGESL-DVLLDRT--PSGLIPYK 383
Query: 379 VKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMNEISESEIPYPHRAGYVFK 409
KSDYVKEPI E GL+GLW+ + A ++ PYGG M+EISESEIP+PHRAG ++K
Sbjct: 384 AKSDYVKEPIPEEGLEGLWEKMHEETAGRAILIFSPYGGIMDEISESEIPFPHRAGNIYK 439
BLAST of Lsi02G009700 vs. NCBI nr
Match:
gi|659068692|ref|XP_008445709.1| (PREDICTED: cannabidiolic acid synthase-like [Cucumis melo])
HSP 1 Score: 446.0 bits (1146), Expect = 7.1e-122
Identity = 248/453 (54.75%), Postives = 310/453 (68.43%), Query Frame = 1
Query: 4 LAFIIVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILNFY 63
LAFI+V + +W S++++ EFL CL HSP SI L+YTP N+S+S +LNF
Sbjct: 11 LAFIVVCSSS-SVWPSASSHVNHEEFLPCLLHHSPDSK-SITKLVYTPINTSYSPVLNFS 70
Query: 64 IRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNVP- 123
IRNLRFST TPKPLLIITP HVQA VIC K HG QIRIRSGGHD+EGLSYV+ P
Sbjct: 71 IRNLRFSTPNTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGLSYVAYHPF 130
Query: 124 ----------------------------------IAEKSKTLAFPAGGCPTVGVGGHLSG 183
IAEKS+TLAFPAG CPTVGVGGH SG
Sbjct: 131 VVVDLINLSSVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTVGVGGHFSG 190
Query: 184 GGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKIK 243
GG G LLRKYGLAAD+VIDA +VDANG+ DRKSMGEDLFWAIRGGGGGSFGIV+AWK+K
Sbjct: 191 GGFGTLLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVK 250
Query: 244 LLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLKKGGIRRTRT 303
L+ VP TVT+ T+++ L++ +KLI QWQYVA+KLD+ D F NL G I
Sbjct: 251 LVPVPATVTICTISRTLEEEAIKLIDQWQYVANKLDE-----DLFLGINLLGGKIVAQGD 310
Query: 304 RTVN--ASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESLE 363
+ +N A FFSLFLG+A+EL++I+ K+FP+LGL KE+C E +WIES++ NG +E
Sbjct: 311 K-INPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTG---NGLQIE 370
Query: 364 ----EALLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGG 416
E LLNR+ P G K+KSDYVKEPIS+ +K +W+ L++ +IE A++V +PYGG
Sbjct: 371 DQPLEVLLNRT--PLANGNIKMKSDYVKEPISKATIKEVWQRLKSQDIEGANLVFIPYGG 430
BLAST of Lsi02G009700 vs. NCBI nr
Match:
gi|778661997|ref|XP_004151192.2| (PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus])
HSP 1 Score: 439.5 bits (1129), Expect = 6.6e-120
Identity = 237/450 (52.67%), Postives = 307/450 (68.22%), Query Frame = 1
Query: 5 AFIIVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILNFYI 64
+ I++ + + +W S++ + K FLQCLS HS K I +++TP NSS+S +LNF I
Sbjct: 11 SLILIVVSSSSLWVSASPH-KHHNFLQCLSEHSSKSYP-ISKVVHTPINSSYSSVLNFSI 70
Query: 65 RNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNVP-- 124
RNLRFS TPKPLLIITP H++A VIC K HG QIR RSGGHDYEGLSYV++ P
Sbjct: 71 RNLRFSKPETPKPLLIITPSHVSHIRAAVICSKSHGLQIRTRSGGHDYEGLSYVASHPFI 130
Query: 125 ---------------------------------IAEKSKTLAFPAGGCPTVGVGGHLSGG 184
I EKS+TLAFPAG CPTVGVGGH SGG
Sbjct: 131 ILDLINLRSMKIDVESNTAWVESGTTLGELYYRIGEKSRTLAFPAGICPTVGVGGHFSGG 190
Query: 185 GHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKIKL 244
G+G +LRK+GLAAD+VIDA LVDANGK+ DR+SMGEDLFWAIRGGGGGSFGIV+AWKIKL
Sbjct: 191 GYGLMLRKFGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKIKL 250
Query: 245 LYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLKKGGIRRTRTR 304
+ VP TVT+ + L + +KL+HQWQYVA+KLD+NL F L G I T+
Sbjct: 251 VSVPATVTMCNTYRNLKGDGIKLVHQWQYVANKLDENL-----FLGIILTGGNITTTQEG 310
Query: 305 TVN--ASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIES-ILTLAGFTNGESLE 364
N A+FFSLFLG+ NEL++ + +FPELGL+K+DCIE +WIES ++ G ESL
Sbjct: 311 ITNPIATFFSLFLGKVNELVATLSTTFPELGLIKQDCIETSWIESTLIASTGVQTVESL- 370
Query: 365 EALLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMNE 417
E LLNR+ PS + K+KSDY+KEPIS ++G+W+ L+ +IE++ ++ +PYGG+M++
Sbjct: 371 EPLLNRT--PSNLENEKIKSDYIKEPISIATIEGIWQRLKAQDIETSQLIFIPYGGRMSQ 430
BLAST of Lsi02G009700 vs. NCBI nr
Match:
gi|1009107978|ref|XP_015882468.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Ziziphus jujuba])
HSP 1 Score: 439.5 bits (1129), Expect = 6.6e-120
Identity = 236/446 (52.91%), Postives = 300/446 (67.26%), Query Frame = 1
Query: 4 LAFIIVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILNFY 63
LAF+I+ ++ + + + FLQCLS SP +I LIYTPT+ S+S I NF
Sbjct: 4 LAFVILLSISGAAFSAETHEN----FLQCLS-QSPNAS-AISQLIYTPTHPSYSSIFNFS 63
Query: 64 IRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNVP- 123
I+N RFS+ +TPKPL+I+TPL HV A + C + HG QIR+RSGGHDYEGLSYVS+VP
Sbjct: 64 IQNPRFSSPSTPKPLVILTPLHVSHVPAAINCSRHHGMQIRVRSGGHDYEGLSYVSDVPF 123
Query: 124 ----------------------------------IAEKSKTLAFPAGGCPTVGVGGHLSG 183
IAEKS+TL FPA PTVGVGGHLSG
Sbjct: 124 LVIDLINLTSITVDLEESTAWIEAGSTIGEVYYRIAEKSRTLGFPAAIIPTVGVGGHLSG 183
Query: 184 GGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKIK 243
GG+G +LRKYG+AA+HV+DA +VDA G+ LDR+SMGEDLFWAIRGGGG SFG+VLAWKIK
Sbjct: 184 GGYGTMLRKYGIAAEHVVDAHIVDAEGRFLDRESMGEDLFWAIRGGGGASFGVVLAWKIK 243
Query: 244 LLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLKKGGIRRTRT 303
LL VP TVTVFTVN+ L+QN KL+H+WQ VA KLD++L + + N + G
Sbjct: 244 LLPVPSTVTVFTVNRNLEQNATKLVHRWQNVADKLDEDLFIRVILQSVNTGEEG-----K 303
Query: 304 RTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESLEEA 363
+T+ ASF SLFLG + L+ +M+KSFPELGL K+DC E +WIES L +GF +GESLE
Sbjct: 304 KTIQASFNSLFLGGVDRLLPLMEKSFPELGLTKQDCNEMSWIESTLYFSGFPSGESLEVL 363
Query: 364 LLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMNEIS 415
L R + I K+KSDYVKEPISET L+G+W+ + + A ++ +PYGGKM E+S
Sbjct: 364 LDRRPSR--VILPFKIKSDYVKEPISETSLEGIWEKIYEEEVGKAMLIFVPYGGKMKEVS 423
BLAST of Lsi02G009700 vs. NCBI nr
Match:
gi|449435880|ref|XP_004135722.1| (PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus])
HSP 1 Score: 438.3 bits (1126), Expect = 1.5e-119
Identity = 241/447 (53.91%), Postives = 292/447 (65.32%), Query Frame = 1
Query: 8 IVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILNFYIRNL 67
I +LTF W +SA+ + FL CLS HSP SI +IYTPTN S+S +LNF I NL
Sbjct: 11 IFIVLTFA-WAASAHNHE--SFLHCLSHHSPN-TSSISKIIYTPTNPSYSSVLNFSIHNL 70
Query: 68 RFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNVP----- 127
RF++ TPKP +I+TP +QA +IC K GFQIR RSGGHDYEGLSYVS+V
Sbjct: 71 RFTSPKTPKPQVIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDVSFVVVD 130
Query: 128 ------------------------------IAEKSKTLAFPAGGCPTVGVGGHLSGGGHG 187
IAEKSKTL FPAG CPTVGVGGH SGGG+G
Sbjct: 131 LINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYG 190
Query: 188 GLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKIKLLYV 247
+LRK+GLAAD+VIDA +D NGKL DRKSMGED+FWAIRG GG S+GIVLAWKIKLL V
Sbjct: 191 LMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASYGIVLAWKIKLLPV 250
Query: 248 PPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLKKGGIRRTRT---- 307
PP VTVFT+ + L+QN +IH+WQYV+SK DD L F R + GI T +
Sbjct: 251 PPIVTVFTIARTLEQNATDIIHRWQYVSSKQDDKL----FIR---IILTGINTTNSQHGN 310
Query: 308 -RTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESLEE 367
RT+ A+F SLFLG+ EL+ IMQK+FPELGL KEDCIE +WIES+L AGF+ G+ L
Sbjct: 311 KRTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNV 370
Query: 368 ALLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMNEI 415
L R P K KSDYV EPI + GL+G+W+ E+A ++L PYGG M++I
Sbjct: 371 LLDRRPLTPKRF--FKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKI 430
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| THCAS_CANSA | 1.4e-93 | 44.03 | Tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=1 SV=1 | [more] |
| THCAI_CANSA | 1.8e-93 | 43.79 | Inactive tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=3 SV=1 | [more] |
| CASL1_CANSA | 3.4e-92 | 43.14 | Cannabidiolic acid synthase-like 1 OS=Cannabis sativa GN=CBDAS2 PE=2 SV=1 | [more] |
| CASL2_CANSA | 7.6e-92 | 42.92 | Cannabidiolic acid synthase-like 2 OS=Cannabis sativa GN=CBDAS3 PE=2 SV=1 | [more] |
| RETOL_ARATH | 3.8e-91 | 41.41 | Reticuline oxidase-like protein OS=Arabidopsis thaliana GN=At4g20830 PE=1 SV=2 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| B9SAY8_RICCO | 6.7e-119 | 52.13 | Reticuline oxidase, putative OS=Ricinus communis GN=RCOM_1334770 PE=4 SV=1 | [more] |
| B9NA97_POPTR | 4.3e-118 | 51.66 | FAD-binding domain-containing family protein OS=Populus trichocarpa GN=POPTR_000... | [more] |
| U5FWX5_POPTR | 5.3e-116 | 51.34 | Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0011s16570g PE=4 SV=1 | [more] |
| A0A061EKB4_THECC | 2.6e-115 | 52.49 | FAD-binding Berberine family protein OS=Theobroma cacao GN=TCM_020191 PE=4 SV=1 | [more] |
| V7CLG0_PHAVU | 2.9e-114 | 50.89 | Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_002G199800g PE=4 SV=1 | [more] |
