Lsi02G007220 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi02G007220
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionPolyphenol oxidase
Locationchr02 : 6520707 .. 6522006 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGAGCCAAAGCGAAATCAGTGATTTTGAAAATGGCATTAGAATGATGAGAGAACTCCCCGACGACGACCCTCGTAGCCTTTTCCATCAAGCTAAAGTTCATTGTGCGTATTGCAATCATGCTTACGAACAATTAGGGTATCTTCCAAAAACTGAATTCCAAGTTCATTCCAACGCCTTATTCTTCCCTTTTCACCGTGCCTACCTATACTTCTTCGAGAGAATCTTGGGGTCTTTAATTGAAAAACCAAATTTCGCCTTACCCTTTTGGAATTGGGACTCATCAGAGGGCATGTGCTTGCCTAGAATGTTCGACAACCCAACATCACCACTCTACAATGAGTTCCGTGATCCACGTCACAATCCACCCAAATTGGTTGACCTCATTTATGATATGAAAACTGAAGACCCTATTTATGACCTCGTGGATAGCAATCTCCATTGGATGAATCACATGTTGGCAGTCACGACCGCTGATGATTTCCTTGGAAAAGTACTCAAAGCCGGAGAAACTCCCAACCACTTCCATATGGGTTTCATTGAAGAGTCCCCCCATGGTAATGTTCACGATTGGGTTGGCAATTATAAACTAATTAATCGTGAAGACATGGGAGTTTTTTACTCTGCAGGGCAAGATCCGGTATTTTATACTCACCACGCCAATGTTGATCGCATGTGGAATATTTGGCAACAAATGATCAATGAAGGAGATCATACCGACCTAGATTGGCTGAATTCCTCGTTTGTGTTTTATGATGAGAATAGCGACGCGGTACGTGTCAAAGTATCGGATTGTTTGGATGGTACAAAACTTGGATATGATTATGATGATGTACCCATTGAATGGATGTACGTGGAGAAAACCTCGCTACCAAATCCATGGATAAGAACTGAAGAACCCGAGCCTCCAATCATCAACTTCCCACAGATTCTAAATTCGAACATAACAACAATGGTAGACAAGCCGGACCGGAGAGCAAAAGCTAGATGTGGTATTGGTGATTGAAGATGTGGAGTACGATCCAACTACACGAATGCATTTTAATGTACTTATTAATGTTGTTAAAGATCGGCCAGACGCGAGCCCCAAGTATCACGAGTTTGTGGGAATCTTTCACAACCTCCCGCATGGTCTCCATCGCCACGAAAAAACCTCTTTGCGTTTTGTGATAACTAAGGCGATTCAAGAGTTGAGTTTGGAGGGTGATAAGAATTTGGCTGTGAGGATTGAACCAAAGGTTGGTGGAGAAAGTGTCACTATTGGAGGGATCAACATTCACCTTGAACCCCTGCAATAA

mRNA sequence

ATGATGAGCCAAAGCGAAATCAGTGATTTTGAAAATGGCATTAGAATGATGAGAGAACTCCCCGACGACGACCCTCGTAGCCTTTTCCATCAAGCTAAAGTTCATTGTGCGTATTGCAATCATGCTTACGAACAATTAGGGTATCTTCCAAAAACTGAATTCCAAGTTCATTCCAACGCCTTATTCTTCCCTTTTCACCGTGCCTACCTATACTTCTTCGAGAGAATCTTGGGGTCTTTAATTGAAAAACCAAATTTCGCCTTACCCTTTTGGAATTGGGACTCATCAGAGGGCATGTGCTTGCCTAGAATGTTCGACAACCCAACATCACCACTCTACAATGAGTTCCGTGATCCACGTCACAATCCACCCAAATTGGTTGACCTCATTTATGATATGAAAACTGAAGACCCTATTTATGACCTCGTGGATAGCAATCTCCATTGGATGAATCACATGTTGGCAGTCACGACCGCTGATGATTTCCTTGGAAAAGTACTCAAAGCCGGAGAAACTCCCAACCACTTCCATATGGGTTTCATTGAAGAGTCCCCCCATGGTAATGTTCACGATTGGGTTGGCAATTATAAACTAATTAATCGTGAAGACATGGGAGTTTTTTACTCTGCAGGGCAAGATCCGGTATTTTATACTCACCACGCCAATGTTGATCGCATGTGGAATATTTGGCAACAAATGATCAATGAAGGAGATCATACCGACCTAGATTGGCTGAATTCCTCGTTTGTGTTTTATGATGAGAATAGCGACGCGGTACGTGTCAAAGTATCGGATTGTTTGGATGGTACAAAACTTGGATATGATTATGATGATGTACCCATTGAATGGATGTACGTGGAGAAAACCTCGCTACCAAATCCATGGATAAGAACTGAAGAACCCGAGCCTCCAATCATCAACTTCCCACAGATTCTAAATTCGAACATAACAACAATGGTAGACAAGCCGGACCGGAGAGCAAAAGCTAGATGTGACGCGAGCCCCAAGTATCACGAGTTTGTGGGAATCTTTCACAACCTCCCGCATGGTCTCCATCGCCACGAAAAAACCTCTTTGCGTTTTGTGATAACTAAGGCGATTCAAGAGTTGAGTTTGGAGGGTGATAAGAATTTGGCTGTGAGGATTGAACCAAAGGTTGGTGGAGAAAGTGTCACTATTGGAGGGATCAACATTCACCTTGAACCCCTGCAATAA

Coding sequence (CDS)

ATGATGAGCCAAAGCGAAATCAGTGATTTTGAAAATGGCATTAGAATGATGAGAGAACTCCCCGACGACGACCCTCGTAGCCTTTTCCATCAAGCTAAAGTTCATTGTGCGTATTGCAATCATGCTTACGAACAATTAGGGTATCTTCCAAAAACTGAATTCCAAGTTCATTCCAACGCCTTATTCTTCCCTTTTCACCGTGCCTACCTATACTTCTTCGAGAGAATCTTGGGGTCTTTAATTGAAAAACCAAATTTCGCCTTACCCTTTTGGAATTGGGACTCATCAGAGGGCATGTGCTTGCCTAGAATGTTCGACAACCCAACATCACCACTCTACAATGAGTTCCGTGATCCACGTCACAATCCACCCAAATTGGTTGACCTCATTTATGATATGAAAACTGAAGACCCTATTTATGACCTCGTGGATAGCAATCTCCATTGGATGAATCACATGTTGGCAGTCACGACCGCTGATGATTTCCTTGGAAAAGTACTCAAAGCCGGAGAAACTCCCAACCACTTCCATATGGGTTTCATTGAAGAGTCCCCCCATGGTAATGTTCACGATTGGGTTGGCAATTATAAACTAATTAATCGTGAAGACATGGGAGTTTTTTACTCTGCAGGGCAAGATCCGGTATTTTATACTCACCACGCCAATGTTGATCGCATGTGGAATATTTGGCAACAAATGATCAATGAAGGAGATCATACCGACCTAGATTGGCTGAATTCCTCGTTTGTGTTTTATGATGAGAATAGCGACGCGGTACGTGTCAAAGTATCGGATTGTTTGGATGGTACAAAACTTGGATATGATTATGATGATGTACCCATTGAATGGATGTACGTGGAGAAAACCTCGCTACCAAATCCATGGATAAGAACTGAAGAACCCGAGCCTCCAATCATCAACTTCCCACAGATTCTAAATTCGAACATAACAACAATGGTAGACAAGCCGGACCGGAGAGCAAAAGCTAGATGTGACGCGAGCCCCAAGTATCACGAGTTTGTGGGAATCTTTCACAACCTCCCGCATGGTCTCCATCGCCACGAAAAAACCTCTTTGCGTTTTGTGATAACTAAGGCGATTCAAGAGTTGAGTTTGGAGGGTGATAAGAATTTGGCTGTGAGGATTGAACCAAAGGTTGGTGGAGAAAGTGTCACTATTGGAGGGATCAACATTCACCTTGAACCCCTGCAATAA

Protein sequence

MMSQSEISDFENGIRMMRELPDDDPRSLFHQAKVHCAYCNHAYEQLGYLPKTEFQVHSNALFFPFHRAYLYFFERILGSLIEKPNFALPFWNWDSSEGMCLPRMFDNPTSPLYNEFRDPRHNPPKLVDLIYDMKTEDPIYDLVDSNLHWMNHMLAVTTADDFLGKVLKAGETPNHFHMGFIEESPHGNVHDWVGNYKLINREDMGVFYSAGQDPVFYTHHANVDRMWNIWQQMINEGDHTDLDWLNSSFVFYDENSDAVRVKVSDCLDGTKLGYDYDDVPIEWMYVEKTSLPNPWIRTEEPEPPIINFPQILNSNITTMVDKPDRRAKARCDASPKYHEFVGIFHNLPHGLHRHEKTSLRFVITKAIQELSLEGDKNLAVRIEPKVGGESVTIGGINIHLEPLQ
Homology
[Close]
BLAST of Lsi02G007220 vs. Swiss-Prot
Match: PPO_MALDO (Polyphenol oxidase, chloroplastic OS=Malus domestica PE=2 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 1.7e-85
Identity = 181/454 (39.87%), Postives = 256/454 (56.39%), Query Frame = 1 

Query: 2   MSQSEISDFENGIRMMRELPDDDPRSLFHQAKVHCAYCNHAYEQLGYLPKTEFQVHSNAL 61
           + Q+    +   + +M+ LPDDDPRS   QA VHCAYC+ AY+Q+G+ P+ E Q+H++ L
Sbjct: 142 VDQAYRDKYYKAMELMKALPDDDPRSFKQQAAVHCAYCDGAYDQVGF-PELELQIHNSWL 201

Query: 62  FFPFHRAYLYFFERILGSLIEKPNFALPFWNWDSSEGMCLPRMFDNPTSPLYNEFRDPRH 121
           FFPFHR YLYFFE+ILG LI  P FALPFWNWDS  GM LP ++ +P SPLY++ R   H
Sbjct: 202 FFPFHRYYLYFFEKILGKLINDPTFALPFWNWDSPAGMPLPAIYADPKSPLYDKLRSANH 261

Query: 122 NPPKLVDLIYDMKTEDPI--YDLVDSNLHWMNHMLAVTT--ADDFLGKVLKAGETPNHFH 181
            PP LVDL Y+  TED +     +++NL  M   +   +  A  F G   +AG+ P+   
Sbjct: 262 QPPTLVDLDYN-GTEDNVSKETTINANLKIMYRQMVSNSKNAKLFFGNPYRAGDEPDP-G 321

Query: 182 MGFIEESPHGNVHDWVGNYKLINREDMGVFYSAGQDPVFYTHHANVDRMWNIWQQMINE- 241
            G IE +PH  VH W G+    N EDMG FYSAG+DP+F+ HH+NVDRMW+IW+ +  + 
Sbjct: 322 GGSIEGTPHAPVHLWTGDNTQPNFEDMGNFYSAGRDPIFFAHHSNVDRMWSIWKTLGGKR 381

Query: 242 GDHTDLDWLNSSFVFYDENSDAVRVKVSDCLDGTKLGYDYDDVPIEWM------------ 301
            D TD DWL+S F+FY+EN++ VRVKV DCL+   LGY Y DV I W+            
Sbjct: 382 TDLTDSDWLDSGFLFYNENAELVRVKVRDCLETKNLGYVYQDVDIPWLSSKPTPRRAKVA 441

Query: 302 ---YVEKTSLPNPWIRTEEPEPPIINFPQILNSNITTMVDKPDRRAKAR----------- 361
                +K  + +  + +         FP  L S I+T+V +P ++ +++           
Sbjct: 442 LSKVAKKLGVAHAAVASSSKVVAGTEFPISLGSKISTVVKRPKQKKRSKKAKEDEEEILV 501

Query: 362 -------CDASPKYHEFV----------------GIFHNLPHGLHRHEK---TSLRFVIT 399
                   D + K+  +V                G F ++PH  H+H+K   T LR  +T
Sbjct: 502 IEGIEFDRDVAVKFDVYVNDVDDLPSGPDKTEFAGSFVSVPHS-HKHKKKMNTILRLGLT 561

[Close]
BLAST of Lsi02G007220 vs. Swiss-Prot
Match: PPO2_IPOBA (Polyphenol oxidase II, chloroplastic OS=Ipomoea batatas GN=co-2 PE=2 SV=2)

HSP 1 Score: 300.4 bits (768), Expect = 2.9e-80
Identity = 163/353 (46.18%), Postives = 211/353 (59.77%), Query Frame = 1 

Query: 2   MSQSEISDFENGIRMMRELPDDDPRSLFHQAKVHCAYCNHAYEQLGYLPKTEFQVHSNAL 61
           M +  I+ FE  IR+M+ELP DDPR+ + QA VHCAYCN  Y Q  Y P  E QVH++ L
Sbjct: 145 MDKEYIAKFEKAIRLMKELPADDPRNFYQQALVHCAYCNGGYVQTDY-PDKEIQVHNSWL 204

Query: 62  FFPFHRAYLYFFERILGSLIEKPNFALPFWNWDSSEGMCLPRMFDNPTSPLYNEFRDPRH 121
           FFPFHR YLYF+ERILG LI  P F LPFWNWD+  GM +P+ F N  SPLY+E R+  H
Sbjct: 205 FFPFHRWYLYFYERILGKLIGDPTFGLPFWNWDTPAGMLIPQYFRNQNSPLYDENRNQSH 264

Query: 122 NPPKLVDLIY-DMKTEDPIYDLVDSNLHWMNHMLAVT--TADDFLGKVLKAGETPNHFHM 181
            P  ++DL Y    T+    + + +NL  M   +     TA+ FLGK  KAG+ P +   
Sbjct: 265 LP-LVMDLGYAGTDTDVTDQERISNNLALMYKSMVTNAGTAELFLGKPYKAGDDPVNKGG 324

Query: 182 GFIEESPHGNVHDWVGNYK--LINREDMGVFYSAGQDPVFYTHHANVDRMWNIWQQMINE 241
           G IE  PH  VH WVG+ K    N EDMG FYSAG+D +FY HH+NVDRMW IWQQ+  +
Sbjct: 325 GSIENIPHTPVHRWVGDVKPRTQNGEDMGNFYSAGRDILFYCHHSNVDRMWTIWQQLGGK 384

Query: 242 G---DHTDLDWLNSSFVFYDENSDAVRVKVSDCLDGTKLGYDYDDVPIEWMYVEKTSLPN 301
           G   D TD DWL+++F+FYDEN  AVRV+V D LD  KLGY Y+   + W+  +      
Sbjct: 385 GRRRDFTDSDWLDATFIFYDENKQAVRVRVGDALDNQKLGYKYEFTNLPWLNSKPLPTKK 444

Query: 302 PWIRTEEPEPPIIN--FPQILNSNITTMVDKPDR-RAKARCDASPKYHEFVGI 344
                   + P +   FP  L+  +   V +P + R+K   +A  +  E  GI
Sbjct: 445 KTGLAARSKAPFVTDVFPLTLDKVVQVKVPRPKKSRSKEEKEAEEEILEIQGI 495

[Close]
BLAST of Lsi02G007220 vs. Swiss-Prot
Match: LAHY_LARTR ((+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata PE=1 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 1.4e-79
Identity = 154/352 (43.75%), Postives = 215/352 (61.08%), Query Frame = 1 

Query: 7   ISDFENGIRMMRELPDDDPRSLFHQAKVHCAYCNHAYEQLGYLPKTEFQVHSNALFFPFH 66
           I  ++  +  M+ LP +DPRS + QA +HCAYCN AY+Q+G+ P    QVH + LF PFH
Sbjct: 139 IEKYKKALAAMKALPQNDPRSFYQQANIHCAYCNGAYDQVGF-PDVNIQVHHSWLFLPFH 198

Query: 67  RAYLYFFERILGSLIEKPNFALPFWNWDSSEGMCLPRMFDNPTSPLYNEFRDPRHNPPKL 126
           R YLYF+ERILGSLI+ P FA+PFWNWD+ +GM +P MF +P SPLY+  R+P H P  +
Sbjct: 199 RWYLYFYERILGSLIDDPTFAIPFWNWDAPKGMHMPHMFIDPNSPLYDAKRNPAHFPDTI 258

Query: 127 VDLIYDMKTEDPIYD--LVDSNLHWM-NHMLAVTTADDFLGKVLKAGETPNHFHMGFIEE 186
           VDL +    E P ++   + +NL  M   ++    A  F G+ L+AG  P+    G +E 
Sbjct: 259 VDLDFS-SGEAPSHNPRQIGNNLSIMYKQVVRAKKARLFHGRPLQAGSFPDESGDGSLEG 318

Query: 187 SPHGNVHDWVGNYKLINREDMGVFYSAGQDPVFYTHHANVDRMWNIWQQMINEGDHTDLD 246
           +PHGN+H W G+ +  N E+MG FYSAG+DP+FY HHANVDRMW IW+  +   D+   D
Sbjct: 319 TPHGNIHLWSGDPRQSNFENMGNFYSAGRDPLFYAHHANVDRMWYIWKNSLGRKDYKKKD 378

Query: 247 WLNSSFVFYDENSDAVRVKVSDCLDGTKLGYDYDDVPIEWMYVE-KTSLPNPW---IRTE 306
           WLN+ F+ +DEN+  VRV V D LD  KLGY Y +V I W+  + K    NPW   +R++
Sbjct: 379 WLNAGFLLFDENAQPVRVYVRDALDERKLGYAYQEVDIPWINSKPKPRKANPWPNRLRSK 438

Query: 307 EPEPP--IINFPQILNSNITTMVDKPDRRAKARCDASPKYHEFVGIFHNLPH 350
                  I  FP  L+S ++  V +P    K+R  +  +  E V +   + H
Sbjct: 439 ATTTTKLINKFPLTLDSTVSFEVKRP---KKSRSKSEKEDEEEVLVIEKIKH 485

[Close]
BLAST of Lsi02G007220 vs. Swiss-Prot
Match: PPO1_IPOBA (Polyphenol oxidase I, chloroplastic OS=Ipomoea batatas GN=co-1 PE=1 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 1.6e-78
Identity = 149/336 (44.35%), Postives = 202/336 (60.12%), Query Frame = 1 

Query: 2   MSQSEISDFENGIRMMRELPDDDPRSLFHQAKVHCAYCNHAYEQLGYLPKTEFQVHSNAL 61
           M +  I+ F   + +M+ LP DDPR+ + QA VHCAYCN  Y+Q+ + P  E QVH++ L
Sbjct: 55  MDKDAIAKFAKAVELMKALPADDPRNFYQQALVHCAYCNGGYDQVNF-PDQEIQVHNSWL 114

Query: 62  FFPFHRAYLYFFERILGSLIEKPNFALPFWNWDSSEGMCLPRMFDNPTSPLYNEFRDPRH 121
           FFPFHR YLYF+ERILG LI  P+F LPFWNWD+  GM LP   ++ TS LY+  R+  H
Sbjct: 115 FFPFHRWYLYFYERILGKLIGDPSFGLPFWNWDNPGGMVLPDFLNDSTSSLYDSNRNQSH 174

Query: 122 NPPKLVDLIYD-MKTEDPIYDLVDSNLHWMNHMLAVT--TADDFLGKVLKAGETPNHFHM 181
            PP +VDL Y+   T+      +  NL  M   +     TA+ FLGK  +AG+ P+    
Sbjct: 175 LPPVVVDLGYNGADTDVTDQQRITDNLALMYKQMVTNAGTAELFLGKAYRAGDAPSP-GA 234

Query: 182 GFIEESPHGNVHDWVGNYKLINREDMGVFYSAGQDPVFYTHHANVDRMWNIWQQMI---N 241
           G IE SPH  +H WVG+ +  N EDMG FYSAG+D  FY HH+NVDRMW IWQQ+     
Sbjct: 235 GSIETSPHIPIHRWVGDPRNTNNEDMGNFYSAGRDIAFYCHHSNVDRMWTIWQQLAGKPR 294

Query: 242 EGDHTDLDWLNSSFVFYDENSDAVRVKVSDCLDGTKLGYDYDDVPIEWMYVEKTSLPNPW 301
           + D+TD DWLN++F+FYDEN  AV+V++ D LD  K+GY Y   P+ W+  +        
Sbjct: 295 KRDYTDSDWLNATFLFYDENGQAVKVRIGDSLDNQKMGYKYAKTPLPWLDSKPVPTKKKG 354

Query: 302 IRTEEPEPPIIN--FPQILNSNITTMVDKPDRRAKA 330
               + + P +   FP  L+  +   V +P +   A
Sbjct: 355 GYASKSKAPFVASVFPVTLDKVVQVKVARPKKSRSA 388

[Close]
BLAST of Lsi02G007220 vs. Swiss-Prot
Match: PPO_VICFA (Polyphenol oxidase A1, chloroplastic OS=Vicia faba PE=1 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 4.3e-76
Identity = 146/343 (42.57%), Postives = 205/343 (59.77%), Query Frame = 1 

Query: 7   ISDFENGIRMMRELPDDDPRSLFHQAKVHCAYCNHAYEQLGYLPKTEFQVHSNALFFPFH 66
           +  ++    +M+ LP +DPR+   QA +HCAYC+ AY Q+G+ P  + QVH + LFFPFH
Sbjct: 153 LEKYKKATELMKALPSNDPRNFTQQANIHCAYCDGAYSQIGF-PDLKLQVHGSWLFFPFH 212

Query: 67  RAYLYFFERILGSLIEKPNFALPFWNWDSSEGMCLPRMFDNPTSPLYNEFRDPRHNPPKL 126
           R YLYF+ERILGSLI  P FALPFWN+D+ +GM LP ++ +  SPLY+E R+  H PP L
Sbjct: 213 RWYLYFYERILGSLINDPTFALPFWNYDAPDGMQLPTIYADKASPLYDELRNASHQPPTL 272

Query: 127 VDLIY-DMKTEDPIYDLVDSNLHWMNHMLAVT--TADDFLGKVLKAGETPNHFHMGFIEE 186
           +DL + D+ ++    +L+ +NL  M   +     T+  FLG   +AG+       G IE 
Sbjct: 273 IDLNFCDIGSDIDRNELIKTNLSIMYRQVYSNGKTSRLFLGNPYRAGDAEPQ-GAGSIEN 332

Query: 187 SPHGNVHDWVGNYKLINREDMGVFYSAGQDPVFYTHHANVDRMWNIWQQM-INEGDHTDL 246
            PH  VH W G+    N EDMG+FYSA +DP+FY+HH+NVDR+W IW+ +   + D TD 
Sbjct: 333 VPHAPVHTWTGDNTQTNIEDMGIFYSAARDPIFYSHHSNVDRLWYIWKTLGGKKHDFTDK 392

Query: 247 DWLNSSFVFYDENSDAVRVKVSDCLDGTKLGYDYDDVPIEW---------MYVEK----- 306
           DWL S F+FYDEN + VRV V D LD  KLGY Y DVPI W           V+K     
Sbjct: 393 DWLESGFLFYDENKNLVRVNVKDSLDIDKLGYAYQDVPIPWEKAKPVPRRTKVQKLVEVE 452

Query: 307 ------TSLPNPWIRTEEPEPPIINFPQILNSNITTMVDKPDR 326
                    P  ++  ++     + FP +LN+ ++ +V +P +
Sbjct: 453 VNDGNLRKSPTIFLVRQQSPRKYVTFPLVLNNKVSAIVKRPKK 493

[Close]
BLAST of Lsi02G007220 vs. TrEMBL
Match: A0A0A0LUI1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G073090 PE=4 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 2.0e-144
Identity = 249/436 (57.11%), Postives = 308/436 (70.64%), Query Frame = 1 

Query: 1   MMSQSEISDFENGIRMMRELPDDDPRSLFHQAKVHCAYCNHAYEQLGYLPKTEFQVHSNA 60
           +M+Q++I + E+GI+ +++L + DP S  HQAK+HCAYC++ Y QLG + +T F VHSNA
Sbjct: 124 LMNQTQIEELESGIKKLKQLDEGDPHSFLHQAKIHCAYCDYVYNQLGSITETRFGVHSNA 183

Query: 61  LFFPFHRAYLYFFERILGSLIEKPNFALPFWNWDSSEGMCLPRMFDNPTSPLYNEFRDPR 120
           LFFPFHRAYLYFFERIL   I  PNFA+PFWNWDS  GM +P  ++ P+S LY+  RDPR
Sbjct: 184 LFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMYMPEFYNRPSSSLYDHLRDPR 243

Query: 121 HNPPKLVDLIYDMKTEDPIYDLVDSNLHWMNHMLAVTTADDFLGKVLKAGETPNHFHMGF 180
           H+P KL+ L Y+ KTED  +D+VD NL WMN  + V +   FLG+VLKAGETPN   MG 
Sbjct: 244 HDPHKLIYLNYNGKTEDHSFDVVDCNLRWMNDRMQVQSPRSFLGRVLKAGETPNPTDMGT 303

Query: 181 IEESPHGNVHDWVGNYKLINREDMGVFYSAGQDPVFYTHHANVDRMWNIWQQMI-NEGDH 240
           IE+SPH N+HDW+G  K  + EDMG FYSA  DP+FY HHAN+DR+WNIW+ +  N  DH
Sbjct: 304 IEQSPHNNIHDWLGT-KTPHFEDMGSFYSAALDPLFYAHHANIDRLWNIWKTLDGNPKDH 363

Query: 241 TDLDWLNSSFVFYDENSDAVRVKVSDCLDGTKLGYDYDDVPIEWMYVEKTSLPNPWIRTE 300
            D DWLNSSFVFYDEN +AV +KVSDCLD  KLGY Y +VP+ W+++ K   PNPWIRTE
Sbjct: 364 IDPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLGYVYQEVPLPWLHMNKIPTPNPWIRTE 423

Query: 301 EPEPPIINFPQILNSNITTMVDKP--DRRAK----------ARCDASPKYHEFVGI---- 360
           E EPPI+NFPQILNS+ITTMVD+P  DR  +             D + + H  V +    
Sbjct: 424 ESEPPIVNFPQILNSDITTMVDRPEEDRSGEEVDEVLVLEDVEYDPTTRVHFDVLVNVVD 483

Query: 361 ---------------FHNLPHGLHRHEKTSLRFVITKAIQELSLEGDKNLAVRIEPKVGG 405
                          F N+PHGLH H+KTSLRFVIT+ IQ L LE DKNLAV+IEP VGG
Sbjct: 484 KAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLERDKNLAVKIEPMVGG 543

[Close]
BLAST of Lsi02G007220 vs. TrEMBL
Match: B9VS06_9ERIC (Polyphenoloxidase OS=Camellia nitidissima PE=2 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 7.4e-91
Identity = 191/445 (42.92%), Postives = 249/445 (55.96%), Query Frame = 1 

Query: 7   ISDFENGIRMMRELPDDDPRSLFHQAKVHCAYCNHAYEQLGYLPKTEFQVHSNALFFPFH 66
           I+ F   + +M+ LP+DDPRS   QA VHCAYC+ AY Q+G+ P+ + QVH++ LFFPFH
Sbjct: 153 IAKFSKALALMKALPEDDPRSFKQQANVHCAYCDGAYHQVGF-PELDLQVHNSWLFFPFH 212

Query: 67  RAYLYFFERILGSLIEKPNFALPFWNWDSSEGMCLPRMFDNPTSPLYNEFRDPRHNPPKL 126
           R YL+FFERILGSLI  P+FA+PFWNWDS  GM +P M+ +P SPLY+  RD +H PP L
Sbjct: 213 RYYLHFFERILGSLIGDPSFAIPFWNWDSPNGMQMPAMYADPNSPLYDVLRDAKHQPPTL 272

Query: 127 VDLIY---DMKTEDPIYDLVDSNLHWMNHMLAVTTADDFLGKVLKAGETPNHFHMGFIEE 186
           VDL Y   D  T D      + N+ +   + +  +A  FLG   +AG+ P+    G +E 
Sbjct: 273 VDLDYNGSDPTTTDAQQKSSNLNVMYRQMVSSSKSARLFLGSPYRAGDEPDP-GAGSLEN 332

Query: 187 SPHGNVHDWVGNYKLINREDMGVFYSAGQDPVFYTHHANVDRMWNIWQQMINE-GDHTDL 246
            PHG VH W G+    N EDMG FYSAG+D +FY HHANVDRMW+IW+ +  +  D TD 
Sbjct: 333 IPHGPVHIWCGDRTQPNLEDMGNFYSAGRDSIFYGHHANVDRMWSIWKTLGGKRQDFTDP 392

Query: 247 DWLNSSFVFYDENSDAVRVKVSDCLDGTKLGYDYDDVPIEWMYV----------EKTSLP 306
           DWLN+ FVFYDEN+  VRVKV DCLD  KLGY Y DV I W+             K +  
Sbjct: 393 DWLNAGFVFYDENAQLVRVKVKDCLDSRKLGYVYQDVDIPWLNTRPAPRVSKLSRKINKA 452

Query: 307 NPWIRTEEPEPPIINFPQILNSNITTMVDKP-----------------------DRRAKA 366
              +  + P      FP+ L+  +  MV +P                       DR    
Sbjct: 453 GVAMAADTPSTAAEVFPKKLDKAVRVMVPRPKKSRSKKEKDDEEEILVIEGIEVDRDVFV 512

Query: 367 RCDA----------SPKYHEFVGIFHNLPHGLHRHE---KTSLRFVITKAIQELSLEGDK 402
           + D            P   EF G F N+PH  H+H    KT LR  IT+ ++EL  E D 
Sbjct: 513 KFDVFINDEHEVVIGPNDTEFAGSFVNVPH-KHKHGKKIKTILRLGITELLEELGAEDDD 572

[Close]
BLAST of Lsi02G007220 vs. TrEMBL
Match: A0A0B2P669_GLYSO (Polyphenol oxidase II, chloroplastic OS=Glycine soja GN=glysoja_042110 PE=4 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 1.5e-88
Identity = 180/410 (43.90%), Postives = 236/410 (57.56%), Query Frame = 1 

Query: 1   MMSQSEISDFENGIRMMRELPDDDPRSLFHQAKVHCAYCNHAYEQLGYLPKTEFQVHSNA 60
           M+ +  I+  E GI +M+ LPDDDPR+   QAKVHCAYCN AY        T+  +H + 
Sbjct: 1   MVDEEYITKLEKGIALMKALPDDDPRNFIQQAKVHCAYCNGAYHLRHPFQDTKLNIHRSW 60

Query: 61  LFFPFHRAYLYFFERILGSLIEKPNFALPFWNWDSSEGMCLPRMFDNPTSPLYNEFRDPR 120
            FFPFHR YLYFFER LG LI  PNFALPFWNWDS EGM +P  F+NP S LY++ R+  
Sbjct: 61  FFFPFHRWYLYFFERTLGKLIGDPNFALPFWNWDSVEGMQIPSYFNNPNSSLYHQLRNQN 120

Query: 121 HNPPKLVDLIY---DMKTEDPIYDLVDSNLHWM-NHMLAVTTADDFLGKVLKAGETPNHF 180
           H PP +VDL Y   D   + P +  V  NL +M   M+  +T + F+G   + G+ P   
Sbjct: 121 HLPPHVVDLNYNKLDPNDDTPSHQQVSYNLAFMYKQMVLASTKELFMGSPFRLGDNPTP- 180

Query: 181 HMGFIEESPHGNVHDWVGNYKLINREDMGVFYSAGQDPVFYTHHANVDRMWNIWQQMINE 240
            MG IE +PH  VH WVG     + EDMG FY+A +D +FY HH N DR+W +W+ +  E
Sbjct: 181 GMGSIEAAPHNTVHTWVGAADKPHHEDMGAFYTAARDLIFYAHHLNSDRLWGLWKTL--E 240

Query: 241 GDHTDL----DWLNSSFVFYDENSDAVRVKVSDCLDGTKLGYDYDDVPIEWMYVEKTSLP 300
           G   D     +WL+S F FYDEN++ VRVKV DCLD  KLGY Y+DV + W+    TS  
Sbjct: 241 GRRKDYSDDPNWLDSDFYFYDENANFVRVKVRDCLDTKKLGYVYEDVDLPWLRTPPTSPK 300

Query: 301 NPWIRTEEPEPPIINFPQILNSNITTMVDKPDRRAKARCDASPKYHEFVGIFHNLPHGLH 360
           +  +R  +  P  +   Q ++ N+  M     +   A   + P   EFVG F NL HG  
Sbjct: 301 SKLLRKAKKSP--LPSHQQVSYNLAFMY---KQMVLANNLSEPDQTEFVGTFVNLFHGQG 360

Query: 361 RHEKTSLRFVITKAIQELSLEGDKNLAVRIEPKVGGESVTIGGINIHLEP 403
            +  TS +  I+K ++ L  E D  + V + PKVG   V IGGI I   P
Sbjct: 361 HNINTSFKVGISKVLECLEAEEDDVVLVTLVPKVGKGDVIIGGIKIEFIP 402

[Close]
BLAST of Lsi02G007220 vs. TrEMBL
Match: H2FH89_9ROSI (Polyphenol oxidase OS=Canarium album GN=ppo2 PE=2 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 2.0e-88
Identity = 190/463 (41.04%), Postives = 251/463 (54.21%), Query Frame = 1 

Query: 1   MMSQSEISDFENGIRMMRELPDDDPRSLFHQAKVHCAYCNHAYEQLGYLPKTEFQVHSNA 60
           ++  + I+ +   I +M+ LP DDPR    QA VHCAYC+ AY+Q+G+ P  E QVH++ 
Sbjct: 153 LVDDAYIAKYSRAIELMKALPADDPRGFMQQANVHCAYCDGAYDQIGF-PNLELQVHNSW 212

Query: 61  LFFPFHRAYLYFFERILGSLIEKPNFALPFWNWDSSEGMCLPRMFDNPTSPLYNEFRDPR 120
           LFFPFHR YLYF+E+ILG LIE P FALPFWNWDS  GM +P M+ NP SPLY+ FR+  
Sbjct: 213 LFFPFHRYYLYFYEKILGKLIEDPTFALPFWNWDSPPGMQMPAMYANPKSPLYDNFRNAS 272

Query: 121 HNPPKLVDLIYDMKTEDPI-YDLVDSNLHWMNHMLAVT--TADDFLGKVLKAGETPNHFH 180
           H PP LVDL Y+   E     D + SNL  M   +     TA  F G   +AG+ P+   
Sbjct: 273 HQPPTLVDLDYNRTDETTTNKDQLSSNLKIMYRQMVSNGKTAKLFFGNAYRAGDEPDP-G 332

Query: 181 MGFIEESPHGNVHDWVGNYKLINREDMGVFYSAGQDPVFYTHHANVDRMWNIWQQM--IN 240
           +G IE  PHG VH W G+    N EDMG FYSA +DP+FY+HH+N+DRMW IW+ +    
Sbjct: 333 LGSIENIPHGPVHLWCGDKTQPNLEDMGNFYSAARDPIFYSHHSNIDRMWTIWKTLGGKK 392

Query: 241 EGDHTDLDWLNSSFVFYDENSDAVRVKVSDCLDGTKLGYDYDDVPIEWMYVEKTSLPNPW 300
             + TD DWL++ F+FYDEN++ VRVKV DCLD  KLGYDY  V I W+  + T      
Sbjct: 393 RKEFTDPDWLDAGFLFYDENANLVRVKVRDCLDNKKLGYDYQKVDIPWLNTKPT------ 452

Query: 301 IRTEEPEPPIIN------------------------FPQILNSNITTMVDKPDR-RAKA- 360
                P   I+N                        FP +L+  ++T+V +P + R+K  
Sbjct: 453 -----PRKRIVNKVRSTFGVAHAAETRRIQLTARSQFPLVLDKTMSTLVPRPKKSRSKKE 512

Query: 361 ---------------RCDASPKYH----------------EFVGIFHNLPHGLHRHE--- 399
                            DA  K+                 EF G F N+PH  H+H    
Sbjct: 513 KEEEEEVLVIEGIDFERDAQVKFDVYINDEHDVPSGPDDTEFAGSFVNVPH-KHKHGKKI 572

[Close]
BLAST of Lsi02G007220 vs. TrEMBL
Match: A0A0L9U315_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan03g060400 PE=4 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 9.9e-88
Identity = 178/439 (40.55%), Postives = 249/439 (56.72%), Query Frame = 1 

Query: 1   MMSQSEISDFENGIRMMRELPDDDPRSLFHQAKVHCAYCNHAYEQLGYLPKTEFQVHSNA 60
           ++ +  I+ FE GI +M+ LPDDDPRS   QAKVH AYCN AY        T+  +H + 
Sbjct: 123 LLDEEYIAKFEKGIALMKGLPDDDPRSFIQQAKVHSAYCNGAYHLYHPFQDTKLDIHRSW 182

Query: 61  LFFPFHRAYLYFFERILGSLIEKPNFALPFWNWDSSEGMCLPRMFDNPTSPLYNEFRDPR 120
            FFPFHR Y+YFFERILG+LI  P+FALPFWNWDS E M +P  F NP S L++E R+ +
Sbjct: 183 FFFPFHRWYIYFFERILGNLIGDPDFALPFWNWDSIEAMHMPSCFTNPNSSLFHELRNQK 242

Query: 121 HNPPKLVDLIYDMKTED-PIYDLVDSNLHWM-NHMLAVTTADDFLGKVLKAGETPNHFHM 180
           H PP ++DL Y     D P +  V  NL  M   M+  +T + F+G   + G+ P H  +
Sbjct: 243 HLPPHVIDLNYAAGNVDVPSHQQVLYNLATMYKQMVLASTTELFMGSPFRLGDNP-HPGI 302

Query: 181 GFIEESPHGNVHDWVGNYKLINREDMGVFYSAGQDPVFYTHHANVDRMWNIWQQMINEG- 240
           G +E +PH  VH WVG     + EDMG FY+A +DP+FY HH+N+DRMW IW + + EG 
Sbjct: 303 GSVEAAPHNTVHTWVGGADTPHHEDMGTFYTAARDPIFYGHHSNLDRMWAIW-KTLGEGR 362

Query: 241 -DHTDLDWLNSSFVFYDENSDAVRVKVSDCLDGTKLGYDYDDVPIEWMYVEKTSLPNPWI 300
            +++D DWL+S F FYDEN+D VRVKV DC+D   LGY Y DV + W+    TS  +  +
Sbjct: 363 REYSDQDWLDSEFFFYDENADFVRVKVRDCIDTKNLGYVYQDVDLAWLRTPPTSRKSKLL 422

Query: 301 RTEEPEPPII----NFPQILNSNITTMVDKPDRRAKAR---------------------- 360
           R ++    +       P +L+S IT+++ K  ++ ++R                      
Sbjct: 423 RQKKKAQVLSWKPRKLPLVLDS-ITSVIVKRPKKLRSREEKEEEEEVLVIEGIEFGSDKY 482

Query: 361 -------CDASPKYHEFVGIFHNLPHGLHRHEKTSLRFVITKAIQELSLEGDKNLAVRIE 403
                   D  P   EFVG F +L HG   +  TS +  ++K ++ L  E D ++ V + 
Sbjct: 483 VKFDVHVDDDDPDETEFVGSFVHLYHGHGHNISTSFKVGLSKVLENLEAEEDDDVLVTLV 542

[Close]
BLAST of Lsi02G007220 vs. NCBI nr
Match: gi|449457811|ref|XP_004146641.1| (PREDICTED: polyphenol oxidase, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 520.4 bits (1339), Expect = 2.8e-144
Identity = 249/436 (57.11%), Postives = 308/436 (70.64%), Query Frame = 1 

Query: 1   MMSQSEISDFENGIRMMRELPDDDPRSLFHQAKVHCAYCNHAYEQLGYLPKTEFQVHSNA 60
           +M+Q++I + E+GI+ +++L + DP S  HQAK+HCAYC++ Y QLG + +T F VHSNA
Sbjct: 124 LMNQTQIEELESGIKKLKQLDEGDPHSFLHQAKIHCAYCDYVYNQLGSITETRFGVHSNA 183

Query: 61  LFFPFHRAYLYFFERILGSLIEKPNFALPFWNWDSSEGMCLPRMFDNPTSPLYNEFRDPR 120
           LFFPFHRAYLYFFERIL   I  PNFA+PFWNWDS  GM +P  ++ P+S LY+  RDPR
Sbjct: 184 LFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMYMPEFYNRPSSSLYDHLRDPR 243

Query: 121 HNPPKLVDLIYDMKTEDPIYDLVDSNLHWMNHMLAVTTADDFLGKVLKAGETPNHFHMGF 180
           H+P KL+ L Y+ KTED  +D+VD NL WMN  + V +   FLG+VLKAGETPN   MG 
Sbjct: 244 HDPHKLIYLNYNGKTEDHSFDVVDCNLRWMNDRMQVQSPRSFLGRVLKAGETPNPTDMGT 303

Query: 181 IEESPHGNVHDWVGNYKLINREDMGVFYSAGQDPVFYTHHANVDRMWNIWQQMI-NEGDH 240
           IE+SPH N+HDW+G  K  + EDMG FYSA  DP+FY HHAN+DR+WNIW+ +  N  DH
Sbjct: 304 IEQSPHNNIHDWLGT-KTPHFEDMGSFYSAALDPLFYAHHANIDRLWNIWKTLDGNPKDH 363

Query: 241 TDLDWLNSSFVFYDENSDAVRVKVSDCLDGTKLGYDYDDVPIEWMYVEKTSLPNPWIRTE 300
            D DWLNSSFVFYDEN +AV +KVSDCLD  KLGY Y +VP+ W+++ K   PNPWIRTE
Sbjct: 364 IDPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLGYVYQEVPLPWLHMNKIPTPNPWIRTE 423

Query: 301 EPEPPIINFPQILNSNITTMVDKP--DRRAK----------ARCDASPKYHEFVGI---- 360
           E EPPI+NFPQILNS+ITTMVD+P  DR  +             D + + H  V +    
Sbjct: 424 ESEPPIVNFPQILNSDITTMVDRPEEDRSGEEVDEVLVLEDVEYDPTTRVHFDVLVNVVD 483

Query: 361 ---------------FHNLPHGLHRHEKTSLRFVITKAIQELSLEGDKNLAVRIEPKVGG 405
                          F N+PHGLH H+KTSLRFVIT+ IQ L LE DKNLAV+IEP VGG
Sbjct: 484 KAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLERDKNLAVKIEPMVGG 543

[Close]
BLAST of Lsi02G007220 vs. NCBI nr
Match: gi|659068847|ref|XP_008446626.1| (PREDICTED: polyphenol oxidase I, chloroplastic-like [Cucumis melo])

HSP 1 Score: 486.9 bits (1252), Expect = 3.5e-134
Identity = 239/439 (54.44%), Postives = 301/439 (68.56%), Query Frame = 1 

Query: 1   MMSQSEISDFENGIRMMRELPDDDPRSLFHQAKVHCAYCNHAYEQLGYLPKTEFQVHSNA 60
           +M+Q++I + E GI+ +++L +DDP S  HQAK+HCAYC++AY QLG + +T F+VHSNA
Sbjct: 290 LMNQTQIEELERGIQKLKQLDEDDPHSFSHQAKIHCAYCDYAYNQLGSITETRFEVHSNA 349

Query: 61  LFFPFHRAYLYFFERILGSLIEKPNFALPFWNWDSSEGMCLPRMFDNPTSPLYNEFRDPR 120
           LFFPFHRAYLYFFERIL   I  P+FA+PFWNWDS  GM +P  ++ P+S LY+  RDPR
Sbjct: 350 LFFPFHRAYLYFFERILRHYIGNPDFAIPFWNWDSPPGMHMPEFYNRPSSSLYDNLRDPR 409

Query: 121 HNPPKLVDLIYDMKTEDPIYDLVDSNLHWMNHMLAVTTADDFLGKVLKAGETPNHFHMGF 180
           H+P KL+DL Y+ KTED  +D+VD NL WMN  + V +   FLG+VLKAGETP    MG 
Sbjct: 410 HDPHKLIDLNYNGKTEDHSFDVVDCNLRWMNDRMKVQSPRSFLGRVLKAGETPKPSDMGT 469

Query: 181 IEESPHGNVHDWVGNYKLINREDMGVFYSAGQDPVFYTHHANVDRMWNIWQQMI-NEGDH 240
           IE+SPH N+HDWVG  K  + EDMG FYSA  DP+FY HH N+DR+WNIW+ +  N  DH
Sbjct: 470 IEQSPHNNIHDWVGT-KTPHFEDMGSFYSAALDPLFYAHHTNIDRLWNIWKTLDGNPKDH 529

Query: 241 TDLDWLNSSFVFYDENSDAVRVKVSDCLDGTKLGYDYDDVPIEWMYVEKTSLPNPWIRTE 300
           TD DWLNSSFVFYDEN +AVR++VSDCLD  KLGY Y +VP+ W+ + K   PNPWIRTE
Sbjct: 530 TDPDWLNSSFVFYDENKNAVRIEVSDCLDTEKLGYVYQEVPLPWLLLNKIPSPNPWIRTE 589

Query: 301 E--------PEP------PIINFPQILNS---------------------NITTMVDKPD 360
           E        P+        +++ P+   S                     +   +V+  D
Sbjct: 590 EPEPPIINFPQTLNSDITTMVDRPEEAGSGEEVDVVLVLEDVEYDPTTRVHFDVLVNVVD 649

Query: 361 RRAKARCDASPKYHEFVGIFHNLPHGLHRHEKTSLRFVITKAIQELSLEGDKNLAVRIEP 404
             A+ RC A   + EFVG F N+PHGLH H+KTSLRFVIT+ IQ L LE DKNLAV+IEP
Sbjct: 650 E-AQDRCAA---FLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLERDKNLAVKIEP 709

[Close]
BLAST of Lsi02G007220 vs. NCBI nr
Match: gi|659068849|ref|XP_008446638.1| (PREDICTED: polyphenol oxidase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 380.6 bits (976), Expect = 3.5e-102
Identity = 199/444 (44.82%), Postives = 265/444 (59.68%), Query Frame = 1 

Query: 1   MMSQSEISDFENGIRMMRELPDDDPRSLFHQAKVHCAYCNHAYEQLGYLPKTEFQVHSNA 60
           +++  +++DFE GI +M+ L  DDPR+ + QAK+HCAYC+ AY+Q+G   +T+F      
Sbjct: 136 LLTPKQLADFERGIEIMKNLKPDDPRNFYQQAKIHCAYCDSAYKQIGS-EQTDFL----- 195

Query: 61  LFFPFHRAYLYFFERILGSLIEKPNFALPFWNWDSSEGMCLPRMFD-NPTSPLYNEFRDP 120
                          IL   I  P+FA+PFWNWD+  GM +P  ++ NP SPLY+  R+P
Sbjct: 196 ---------------ILRHFIGDPDFAIPFWNWDAPIGMHMPIHYNGNPNSPLYDANRNP 255

Query: 121 RHNPPKLVDLIYDMKTEDPIYDLVDSNLHWMNHMLAVTTADDFLGKVLKAGETPNHFHMG 180
            H  PK++DL YD  TE+P YD+VD NL WMN  ++V T   FLGKV K GETP    MG
Sbjct: 256 LHKAPKVLDLNYDGSTENPPYDVVDCNLRWMNKQMSVKTPTRFLGKVCKLGETPTLDGMG 315

Query: 181 FIEESPHGNVHDWVGNYKLI-NREDMGVFYSAGQDPVFYTHHANVDRMWNIWQQMINEGD 240
            IE SPH NVH+WVGNY +  N EDMG FY+A +DP+FY HHANVDR+W IW +M N G 
Sbjct: 316 TIESSPHNNVHNWVGNYPITPNGEDMGAFYAAARDPIFYAHHANVDRVWYIWNKM-NSGR 375

Query: 241 HTDL---DWLNSSFVFYDENSDAVRVKVSDCLDGTKLGYDYDDVPIEWMYVEKTSLPNPW 300
              +   DWLNS+FVFYDE+ +AVR+KVSDCLD   LGY Y DV I W+  ++  +P   
Sbjct: 376 IPYIYRQDWLNSTFVFYDEHRNAVRIKVSDCLDSENLGYVYQDVEIPWLNTKQ--VPRTK 435

Query: 301 IRTEEPEPPIINFPQILNS-----------------------------------NITTMV 360
             T +PEPP+INFPQ L+S                                   +   +V
Sbjct: 436 RSTGQPEPPVINFPQTLSSAITTSVERPSGVEPQENEEEVLVIEDIEYDPTKNLHFNVLV 495

Query: 361 DKPDRRAKARCDASPKYHEFVGIFHNLPHGLHRHEKTSLRFVITKAIQELSLEGDKNLAV 405
           D  D       D  P   EF+G F N+PHG     K+SLRFV+T+AI+ L L   + LAV
Sbjct: 496 DVVDEGD----DVCPGNVEFLGSFKNIPHGSKHEAKSSLRFVLTEAIEALGLHDARKLAV 551

[Close]
BLAST of Lsi02G007220 vs. NCBI nr
Match: gi|222093457|gb|ACM43505.1| (polyphenoloxidase [Camellia nitidissima])

HSP 1 Score: 342.4 bits (877), Expect = 1.1e-90
Identity = 191/445 (42.92%), Postives = 249/445 (55.96%), Query Frame = 1 

Query: 7   ISDFENGIRMMRELPDDDPRSLFHQAKVHCAYCNHAYEQLGYLPKTEFQVHSNALFFPFH 66
           I+ F   + +M+ LP+DDPRS   QA VHCAYC+ AY Q+G+ P+ + QVH++ LFFPFH
Sbjct: 153 IAKFSKALALMKALPEDDPRSFKQQANVHCAYCDGAYHQVGF-PELDLQVHNSWLFFPFH 212

Query: 67  RAYLYFFERILGSLIEKPNFALPFWNWDSSEGMCLPRMFDNPTSPLYNEFRDPRHNPPKL 126
           R YL+FFERILGSLI  P+FA+PFWNWDS  GM +P M+ +P SPLY+  RD +H PP L
Sbjct: 213 RYYLHFFERILGSLIGDPSFAIPFWNWDSPNGMQMPAMYADPNSPLYDVLRDAKHQPPTL 272

Query: 127 VDLIY---DMKTEDPIYDLVDSNLHWMNHMLAVTTADDFLGKVLKAGETPNHFHMGFIEE 186
           VDL Y   D  T D      + N+ +   + +  +A  FLG   +AG+ P+    G +E 
Sbjct: 273 VDLDYNGSDPTTTDAQQKSSNLNVMYRQMVSSSKSARLFLGSPYRAGDEPDP-GAGSLEN 332

Query: 187 SPHGNVHDWVGNYKLINREDMGVFYSAGQDPVFYTHHANVDRMWNIWQQMINE-GDHTDL 246
            PHG VH W G+    N EDMG FYSAG+D +FY HHANVDRMW+IW+ +  +  D TD 
Sbjct: 333 IPHGPVHIWCGDRTQPNLEDMGNFYSAGRDSIFYGHHANVDRMWSIWKTLGGKRQDFTDP 392

Query: 247 DWLNSSFVFYDENSDAVRVKVSDCLDGTKLGYDYDDVPIEWMYV----------EKTSLP 306
           DWLN+ FVFYDEN+  VRVKV DCLD  KLGY Y DV I W+             K +  
Sbjct: 393 DWLNAGFVFYDENAQLVRVKVKDCLDSRKLGYVYQDVDIPWLNTRPAPRVSKLSRKINKA 452

Query: 307 NPWIRTEEPEPPIINFPQILNSNITTMVDKP-----------------------DRRAKA 366
              +  + P      FP+ L+  +  MV +P                       DR    
Sbjct: 453 GVAMAADTPSTAAEVFPKKLDKAVRVMVPRPKKSRSKKEKDDEEEILVIEGIEVDRDVFV 512

Query: 367 RCDA----------SPKYHEFVGIFHNLPHGLHRHE---KTSLRFVITKAIQELSLEGDK 402
           + D            P   EF G F N+PH  H+H    KT LR  IT+ ++EL  E D 
Sbjct: 513 KFDVFINDEHEVVIGPNDTEFAGSFVNVPH-KHKHGKKIKTILRLGITELLEELGAEDDD 572

[Close]
BLAST of Lsi02G007220 vs. NCBI nr
Match: gi|747096995|ref|XP_011096441.1| (PREDICTED: aureusidin synthase-like [Sesamum indicum])

HSP 1 Score: 338.2 bits (866), Expect = 2.0e-89
Identity = 191/452 (42.26%), Postives = 252/452 (55.75%), Query Frame = 1 

Query: 1   MMSQSEISDFENGIRMMRELPDDDPRSLFHQAKVHCAYCNHAYEQLGYLPKTEFQVHSNA 60
           +  ++ I+ F   + +MR LP+DDPRS   QA VHCAYC+ AY+Q+G+ P  E QVH++ 
Sbjct: 138 LADEAYIAKFNKAVELMRALPEDDPRSFKQQASVHCAYCDGAYDQVGF-PNLELQVHNSW 197

Query: 61  LFFPFHRAYLYFFERILGSLIEKPNFALPFWNWDSSEGMCLPRMFDNPTSPLYNEFRDPR 120
           LFFPFHR YLYFFERILG LI+ P FA+PFWNWDS  GM +P M+ NP SPLY+  RD R
Sbjct: 198 LFFPFHRYYLYFFERILGKLIDDPTFAMPFWNWDSPGGMRIPAMYANPNSPLYDSLRDQR 257

Query: 121 HNPPKLVDLIYDMKTEDPIYDLVDS---NLHWMNHMLAVT--TADDFLGKVLKAGETPNH 180
           H PP ++DL Y     DP          NL  M   +     TA  F G   + G+ PN 
Sbjct: 258 HQPPTVLDLNYS--GTDPTISADQQTRRNLTVMYRQMVSNSRTARLFFGSPYRRGDDPNP 317

Query: 181 FHMGFIEESPHGNVHDWVGNYKLINREDMGVFYSAGQDPVFYTHHANVDRMWNIWQQMIN 240
              G IE  PHG VH W G+    N E+MG FYSAG+DP+FY HH+N+DR+W+IW+ +  
Sbjct: 318 -GSGSIENIPHGPVHVWTGDRNQPNFENMGNFYSAGRDPIFYAHHSNIDRLWSIWKTLGG 377

Query: 241 EG-DHTDLDWLNSSFVFYDENSDAVRVKVSDCLDGTKLGYDYDDVPIEWMYVEKTSLPNP 300
              D TD D+L++SFVFYDEN+  VRVKV DCLD TKLGY Y DV I W+    T   + 
Sbjct: 378 RRQDITDRDFLDASFVFYDENAQMVRVKVRDCLDHTKLGYVYQDVEIPWLDSRPTPRVSS 437

Query: 301 WIR---------TEEPEPPIINFPQILNSNITTMVDKP---------------------- 360
            +R           +   P   FP  L+  +  MV +P                      
Sbjct: 438 VLRKLKKLGKANAADTHSPKDVFPATLDKVLKVMVKRPKKKRSKKEKDELEEILVIEGIE 497

Query: 361 -DRRAKARCDA----------SPKYHEFVGIFHNLPHGLHRHEK---TSLRFVITKAIQE 402
            DR   A+ D           +P+  EF G F N+PH  H+H K   T LR  IT+ +++
Sbjct: 498 LDRDVYAKFDVYINDEDDEITTPENTEFAGSFVNVPHK-HKHGKKIKTQLRLSITEIMED 557

The following BLAST results are available for this feature:
BLAST of Lsi02G007220 vs. Swiss-Prot
Match NameE-valueIdentityDescription
PPO_MALDO1.7e-8539.87Polyphenol oxidase, chloroplastic OS=Malus domestica PE=2 SV=1[more]
PPO2_IPOBA2.9e-8046.18Polyphenol oxidase II, chloroplastic OS=Ipomoea batatas GN=co-2 PE=2 SV=2[more]
LAHY_LARTR1.4e-7943.75(+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata PE=1 SV=1[more]
PPO1_IPOBA1.6e-7844.35Polyphenol oxidase I, chloroplastic OS=Ipomoea batatas GN=co-1 PE=1 SV=1[more]
PPO_VICFA4.3e-7642.57Polyphenol oxidase A1, chloroplastic OS=Vicia faba PE=1 SV=1[more]
BLAST of Lsi02G007220 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LUI1_CUCSA2.0e-14457.11Uncharacterized protein OS=Cucumis sativus GN=Csa_1G073090 PE=4 SV=1[more]
B9VS06_9ERIC7.4e-9142.92Polyphenoloxidase OS=Camellia nitidissima PE=2 SV=1[more]
A0A0B2P669_GLYSO1.5e-8843.90Polyphenol oxidase II, chloroplastic OS=Glycine soja GN=glysoja_042110 PE=4 SV=1[more]
H2FH89_9ROSI2.0e-8841.04Polyphenol oxidase OS=Canarium album GN=ppo2 PE=2 SV=1[more]
A0A0L9U315_PHAAN9.9e-8840.55Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan03g060400 PE=4 SV=1[more]
BLAST of Lsi02G007220 vs. TAIR10
Match NameE-valueIdentityDescription
BLAST of Lsi02G007220 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449457811|ref|XP_004146641.1|2.8e-14457.11PREDICTED: polyphenol oxidase, chloroplastic-like [Cucumis sativus][more]
gi|659068847|ref|XP_008446626.1|3.5e-13454.44PREDICTED: polyphenol oxidase I, chloroplastic-like [Cucumis melo][more]
gi|659068849|ref|XP_008446638.1|3.5e-10244.82PREDICTED: polyphenol oxidase, chloroplastic-like [Cucumis melo][more]
gi|222093457|gb|ACM43505.1|1.1e-9042.92polyphenoloxidase [Camellia nitidissima][more]
gi|747096995|ref|XP_011096441.1|2.0e-8942.26PREDICTED: aureusidin synthase-like [Sesamum indicum][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO:0008152metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0004097catechol oxidase activity
GO:0016491oxidoreductase activity
Vocabulary: INTERPRO
TermDefinition
IPR022740Polyphenol_oxidase_C
IPR022739Polyphenol_oxidase_cen
IPR008922Unchr_di-copper_centre
IPR002227Tyrosinase_Cu-bd
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0006771 riboflavin metabolic process
biological_process GO:0046148 pigment biosynthetic process
biological_process GO:0008152 metabolic process
biological_process GO:0006570 tyrosine metabolic process
biological_process GO:0009811 stilbene biosynthetic process
biological_process GO:0009809 lignin biosynthetic process
biological_process GO:0009805 coumarin biosynthetic process
biological_process GO:0009821 alkaloid biosynthetic process
cellular_component GO:0005575 cellular_component
cellular_component GO:0009507 chloroplast
cellular_component GO:0009579 thylakoid
molecular_function GO:0036263 L-DOPA monooxygenase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004503 monophenol monooxygenase activity
molecular_function GO:0004097 catechol oxidase activity
molecular_function GO:0016491 oxidoreductase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G007220.1Lsi02G007220.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002227Tyrosinase copper-binding domainPRINTSPR00092TYROSINASEcoord: 88..93
score: 6.2E-17coord: 185..196
score: 6.2E-17coord: 57..74
score: 6.2E-17coord: 212..230
score: 6.2
IPR002227Tyrosinase copper-binding domainPFAMPF00264Tyrosinasecoord: 26..232
score: 6.2
IPR002227Tyrosinase copper-binding domainPROSITEPS00497TYROSINASE_1coord: 57..74
scor
IPR002227Tyrosinase copper-binding domainPROSITEPS00498TYROSINASE_2coord: 213..224
scor
IPR008922Uncharacterised domain, di-copper centreGENE3DG3DSA:1.10.1280.10coord: 2..284
score: 1.4
IPR008922Uncharacterised domain, di-copper centreunknownSSF48056Di-copper centre-containing domaincoord: 1..284
score: 4.06
IPR022739Polyphenol oxidase, central domainPFAMPF12142PPO1_DWLcoord: 238..284
score: 2.7
IPR022740Polyphenol oxidase, C-terminalPFAMPF12143PPO1_KFDVcoord: 332..399
score: 8.3
NoneNo IPR availablePANTHERPTHR11474TYROSINASEcoord: 7..401
score: 2.1E
NoneNo IPR availablePANTHERPTHR11474:SF33SUBFAMILY NOT NAMEDcoord: 7..401
score: 2.1E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Lsi02G007220Csa1G073090Cucumber (Chinese Long) v2culsiB022
Lsi02G007220CSPI01G11840Wild cucumber (PI 183967)cpilsiB021
Lsi02G007220Cla019478Watermelon (97103) v1lsiwmB184
Lsi02G007220ClCG03G005730Watermelon (Charleston Gray)lsiwcgB184
Lsi02G007220MELO3C002580.2Melon (DHL92) v3.6.1lsimedB189
Lsi02G007220Bhi08G001578Wax gourdlsiwgoB244
Lsi02G007220Bhi11G002579Wax gourdlsiwgoB204
Lsi02G007220Cp4.1LG09g08390Cucurbita pepo (Zucchini)cpelsiB026
Lsi02G007220Cp4.1LG20g04280Cucurbita pepo (Zucchini)cpelsiB417
Lsi02G007220CsGy1G011800Cucumber (Gy14) v2cgyblsiB018
Lsi02G007220Carg14643Silver-seed gourdcarlsiB135
Lsi02G007220Carg21488Silver-seed gourdcarlsiB866
Lsi02G007220CsaV3_1G012000Cucumber (Chinese Long) v3cuclsiB022
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi02G007220Wax gourdlsiwgoB234
Lsi02G007220Bottle gourd (USVL1VR-Ls)lsilsiB066
Lsi02G007220Bottle gourd (USVL1VR-Ls)lsilsiB098
Lsi02G007220Bottle gourd (USVL1VR-Ls)lsilsiB107
Lsi02G007220Cucumber (Gy14) v1cgylsiB023
Lsi02G007220Cucumber (Gy14) v1cgylsiB194
Lsi02G007220Cucurbita maxima (Rimu)cmalsiB020
Lsi02G007220Cucurbita maxima (Rimu)cmalsiB207
Lsi02G007220Cucurbita maxima (Rimu)cmalsiB369
Lsi02G007220Cucurbita maxima (Rimu)cmalsiB414
Lsi02G007220Cucurbita maxima (Rimu)cmalsiB796
Lsi02G007220Cucurbita moschata (Rifu)cmolsiB008
Lsi02G007220Cucurbita moschata (Rifu)cmolsiB197
Lsi02G007220Cucurbita moschata (Rifu)cmolsiB356
Lsi02G007220Cucurbita moschata (Rifu)cmolsiB399
Lsi02G007220Cucurbita pepo (Zucchini)cpelsiB167
Lsi02G007220Cucurbita pepo (Zucchini)cpelsiB402
Lsi02G007220Cucurbita pepo (Zucchini)cpelsiB635
Lsi02G007220Wild cucumber (PI 183967)cpilsiB444
Lsi02G007220Cucumber (Chinese Long) v2culsiB438
Lsi02G007220Melon (DHL92) v3.5.1lsimeB179
Lsi02G007220Melon (DHL92) v3.5.1lsimeB228
Lsi02G007220Watermelon (Charleston Gray)lsiwcgB167
Lsi02G007220Watermelon (Charleston Gray)lsiwcgB170
Lsi02G007220Watermelon (97103) v1lsiwmB200
Lsi02G007220Watermelon (97103) v1lsiwmB219
Lsi02G007220Cucumber (Gy14) v2cgyblsiB163
Lsi02G007220Melon (DHL92) v3.6.1lsimedB233
Lsi02G007220Melon (DHL92) v3.6.1lsimedB239
Lsi02G007220Silver-seed gourdcarlsiB824
Lsi02G007220Silver-seed gourdcarlsiB848
Lsi02G007220Cucumber (Chinese Long) v3cuclsiB194
Lsi02G007220Cucumber (Chinese Long) v3cuclsiB465
Lsi02G007220Watermelon (97103) v2lsiwmbB154
Lsi02G007220Watermelon (97103) v2lsiwmbB163
Lsi02G007220Watermelon (97103) v2lsiwmbB169
Lsi02G007220Watermelon (97103) v2lsiwmbB176