Lsi02G004490 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi02G004490
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Descriptionpectin methylesterase 61
Locationchr02 : 3750459 .. 3751677 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAACCGCAGCCGCTCCAGTACATTCGTGTGAGTCCTGAAACTTACAATGAACGTATTGAAATTCCATCTACCAAGAAGTTTATTACTTTGATTGGCGATAATGCACTCACAACCATTATCGTCGACGATAGAAGCAACGCTCGTGGGTTTAAGACCAGTGACTCTGCAACTCTGAGTAAGTGTGTATTTTTATTTTTTTTTAATATATATTATGTCTTATTCTTATCGCTCAATACTCACTAACACACTGTTCCTTTAGATGTTCTGATTTCAATTTTCTTTTTAGTAATTCGGCTTTCTTTTTTTTTATTATTATTTTGGTTGTTTTTAATTTTGAAAAGTGGTGCTTTTGAATTTGGATTTAAATGCATGAGATACAGAAATTTGTATGATAGAAAGTTAGAAACTTGACATTTTAGTTCCGTCGGTCCTTTATTATTATTTTTTCATTATTTAATTTATTTTTTAAGGCAACGTCTTTTATTTATTATTTATTATTTATTATTTTTATTAATGAATCCTTGTGAAGTATAATGCAGTTTGATCAGATTTTAAAATGAGATTACTCCAAATAAATTTTAGATAGAAACAGGAGAAAAATCAAATTTGAACTCTGTTTTCGGTAAACTCTGTTGTTGGGTAAAGCAATAGGCAGTCTATTAATTTCTATTGAACTTCTTGACAGATGGGATTTCAATACGTTTATTATAAGTGCCACGACTAACACGAATGTAGAATCGTGTTTTAGAATGGTCTGGAGCGGCTGCGATTGCATCGCTAATGCTTTTGAAATCGGCAGATCCATCTAACGACACCATTGTATTAAAATTGATGGAATCAATAAGTACTGGAGAATTGAAGATACAAAAAAACAGAACATGCAACAAGCTGGAAAAATGCCATATCTTTCAAGAAGAAGAAGAAGAAGAAAAAAAGGCAATCAAATTAAAAGGACACAGAAGAACTGAGAGAGGAAGAGGGCTTGTTGAAGCTCAAATTCAAAAGCAAAGTGTATATTTGGTGAGGTTTTTAGCCTCAATGGGAACCAACATATTATATTTAATTGAAAGTACTCTTTAATTATTAATTGAATAATTTCTTTTGTTATAAGTTGAAATTGGTTTGAGTTAATTGAGAAAATTATGAGGAAAAGGAATCTTTTTTGTCTTTTGGTATATTGAAAAATATTATTGAAATAAAAAAAGGGTAAAATGTG

mRNA sequence

ATGCAACCGCAGCCGCTCCAGTACATTCGTGTGAGTCCTGAAACTTACAATGAACGTATTGAAATTCCATCTACCAAGAAGTTTATTACTTTGATTGGCGATAATGCACTCACAACCATTATCGTCGACGATAGAAGCAACGCTCGTGGGTTTAAGACCAGTGACTCTGCAACTCTGAGTAAATGGGATTTCAATACGTTTATTATAAGTGCCACGACTAACACGAATGTAGAATCGTGTTTTAGAATGGTCTGGAGCGGCTGCGATTGCATCGCTAATGCTTTTGAAATCGGCAGATCCATCTAACGACACCATTGTATTAAAATTGATGGAATCAATAAGTACTGGAGAATTGAAGATACAAAAAAACAGAACATGCAACAAGCTGGAAAAATGCCATATCTTTCAAGAAGAAGAAGAAGAAGAAAAAAAGGCAATCAAATTAAAAGGACACAGAAGAACTGAGAGAGGAAGAGGGCTTGTTGAAGCTCAAATTCAAAAGCAAAGTGTATATTTGGTGAGGTTTTTAGCCTCAATGGGAACCAACATATTATATTTAATTGAAAGTACTCTTTAATTATTAATTGAATAATTTCTTTTGTTATAAGTTGAAATTGGTTTGAGTTAATTGAGAAAATTATGAGGAAAAGGAATCTTTTTTGTCTTTTGGTATATTGAAAAATATTATTGAAATAAAAAAAGGGTAAAATGTG

Coding sequence (CDS)

ATGCAACCGCAGCCGCTCCAGTACATTCGTGTGAGTCCTGAAACTTACAATGAACGTATTGAAATTCCATCTACCAAGAAGTTTATTACTTTGATTGGCGATAATGCACTCACAACCATTATCGTCGACGATAGAAGCAACGCTCGTGGGTTTAAGACCAGTGACTCTGCAACTCTGAGTAAATGGGATTTCAATACGTTTATTATAAGTGCCACGACTAACACGAATGTAGAATCGTGTTTTAGAATGGTCTGGAGCGGCTGCGATTGCATCGCTAATGCTTTTGAAATCGGCAGATCCATCTAA

Protein sequence

MQPQPLQYIRVSPETYNERIEIPSTKKFITLIGDNALTTIIVDDRSNARGFKTSDSATLSKWDFNTFIISATTNTNVESCFRMVWSGCDCIANAFEIGRSI
Homology
[Close]
BLAST of Lsi02G004490 vs. TrEMBL
Match: A0A0A0LR14_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_1G049450 PE=4 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 1.0e-09
Identity = 32/53 (60.38%), Postives = 43/53 (81.13%), Query Frame = 1 

Query: 8   YIRVSPETYNERIEIPSTKKFITLIGDNALTTIIVDDRSNARGFKTSDSATLS 61
           YI V+P TY+E ++IP  KKFI L+GD+  TTII+D+RSN  GFKT++SATL+
Sbjct: 57  YIHVAPGTYHETLQIPRLKKFIALVGDDPFTTIIIDNRSNGTGFKTNNSATLT 109

[Close]
BLAST of Lsi02G004490 vs. TrEMBL
Match: A0A0A0KNI2_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_5G488760 PE=4 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 2.8e-07
Identity = 33/54 (61.11%), Postives = 38/54 (70.37%), Query Frame = 1 

Query: 8   YIRVSPETYNERIEIPSTKKFITLIGDNALTTIIVDDRSNARGFKTSDSATLSK 62
           YI V P TY E IE+P  K  I LIGD+A TTIIV++RSN  G  T+ SATLSK
Sbjct: 59  YIHVKPGTYKEIIEVPYEKTCIALIGDDASTTIIVNNRSNGTGSSTASSATLSK 112

[Close]
BLAST of Lsi02G004490 vs. TrEMBL
Match: W9R0V6_9ROSA (Pectinesterase OS=Morus notabilis GN=L484_012499 PE=4 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 3.1e-06
Identity = 28/52 (53.85%), Postives = 37/52 (71.15%), Query Frame = 1 

Query: 9  IRVSPETYNERIEIPSTKKFITLIGDNALTTIIVDDRSNARGFKTSDSATLS 61
          IR+ P  Y E +EI S   +I+LIGD+  TTII+ DRSN  GFKT+++ TLS
Sbjct: 2  IRIKPGRYKEHVEIESDLTYISLIGDDVTTTIIIFDRSNGSGFKTNETGTLS 53

[Close]
BLAST of Lsi02G004490 vs. TrEMBL
Match: W9QFB9_9ROSA (Pectinesterase OS=Morus notabilis GN=L484_012492 PE=4 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 4.1e-06
Identity = 28/52 (53.85%), Postives = 37/52 (71.15%), Query Frame = 1 

Query: 9   IRVSPETYNERIEIPSTKKFITLIGDNALTTIIVDDRSNARGFKTSDSATLS 61
           IR+ P  Y ER+EI S   +I+LIGD+  TTII  DRSN  GFKT+++ TL+
Sbjct: 67  IRIKPGRYKERVEIESDLTYISLIGDDVTTTIITFDRSNGSGFKTNETGTLT 118

[Close]
BLAST of Lsi02G004490 vs. TrEMBL
Match: A0A0A0L1M3_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_4G260430 PE=4 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 5.3e-06
Identity = 30/53 (56.60%), Postives = 37/53 (69.81%), Query Frame = 1 

Query: 8   YIRVSPETYNERIEIPSTKKFITLIGDNALTTIIVDDRSNARGFKTSDSATLS 61
           YI V P TY E IE+P  K  I LIGD++ TTIIV++RSN  G  T+ SATL+
Sbjct: 52  YIHVKPGTYQEIIEVPYEKTCIALIGDDSSTTIIVNNRSNGTGSSTASSATLT 104

[Close]
BLAST of Lsi02G004490 vs. NCBI nr
Match: gi|659068777|ref|XP_008446175.1| (PREDICTED: pectinesterase 2-like [Cucumis melo])

HSP 1 Score: 90.5 bits (223), Expect = 1.8e-15
Identity = 48/69 (69.57%), Postives = 56/69 (81.16%), Query Frame = 1 

Query: 8  YIRVSPETYNERIEIPSTKKFITLIGDNALTTIIVDDRSNARGFKTSDSATLSKWDFNTF 67
          YIRV+P TY+ER++IP TK FI LIGDNALTTIIVDDRSNARGFKTSDSATL+  + N F
Sbjct: 20 YIRVTPGTYHERLQIPPTKTFIALIGDNALTTIIVDDRSNARGFKTSDSATLTV-NGNNF 79

Query: 68 IISATTNTN 77
          +  + T  N
Sbjct: 80 LAKSLTFEN 87

[Close]
BLAST of Lsi02G004490 vs. NCBI nr
Match: gi|659068775|ref|XP_008446163.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis melo])

HSP 1 Score: 89.4 bits (220), Expect = 4.0e-15
Identity = 48/69 (69.57%), Postives = 56/69 (81.16%), Query Frame = 1 

Query: 8  YIRVSPETYNERIEIPSTKKFITLIGDNALTTIIVDDRSNARGFKTSDSATLSKWDFNTF 67
          YIRV+P TY+ER++IP TK FI LIGDNALTTIIVDDRSNARGFKTSDSATL+  + + F
Sbjct: 30 YIRVTPGTYHERLQIPPTKTFIALIGDNALTTIIVDDRSNARGFKTSDSATLTV-NGDNF 89

Query: 68 IISATTNTN 77
          I  + T  N
Sbjct: 90 IAKSLTFEN 97

[Close]
BLAST of Lsi02G004490 vs. NCBI nr
Match: gi|778708428|ref|XP_011656190.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 41 [Cucumis sativus])

HSP 1 Score: 83.2 bits (204), Expect = 2.9e-13
Identity = 45/69 (65.22%), Postives = 53/69 (76.81%), Query Frame = 1 

Query: 8  YIRVSPETYNERIEIPSTKKFITLIGDNALTTIIVDDRSNARGFKTSDSATLSKWDFNTF 67
          YI V+P TY+E ++IP TK FI LIGDNALTTIIVDDRSNARGFKT DSATL+  + N F
Sbjct: 30 YIHVTPGTYHECLQIPPTKTFIALIGDNALTTIIVDDRSNARGFKTIDSATLTV-NGNNF 89

Query: 68 IISATTNTN 77
          +  + T  N
Sbjct: 90 LAQSLTFKN 97

[Close]
BLAST of Lsi02G004490 vs. NCBI nr
Match: gi|778657958|ref|XP_004152582.2| (PREDICTED: pectinesterase 2-like [Cucumis sativus])

HSP 1 Score: 70.9 bits (172), Expect = 1.5e-09
Identity = 32/53 (60.38%), Postives = 43/53 (81.13%), Query Frame = 1 

Query: 8   YIRVSPETYNERIEIPSTKKFITLIGDNALTTIIVDDRSNARGFKTSDSATLS 61
           YI V+P TY+E ++IP  KKFI L+GD+  TTII+D+RSN  GFKT++SATL+
Sbjct: 57  YIHVAPGTYHETLQIPRLKKFIALVGDDPFTTIIIDNRSNGTGFKTNNSATLT 109

[Close]
BLAST of Lsi02G004490 vs. NCBI nr
Match: gi|659131264|ref|XP_008465596.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 47, partial [Cucumis melo])

HSP 1 Score: 67.8 bits (164), Expect = 1.3e-08
Identity = 36/64 (56.25%), Postives = 46/64 (71.88%), Query Frame = 1 

Query: 13 PETYNERIEIPSTKKFITLIGDNALTTIIVDDRSNARGFKTSDSATLSKWDFNTFIISAT 72
          P TY+E I+IP  KKFI LIGD+A TTII+D+RSN  GFKT++SATL+    N F+  + 
Sbjct: 1  PGTYHEAIQIPKAKKFIALIGDDAFTTIIIDNRSNGTGFKTNNSATLTV-QGNNFMAKSL 60

Query: 73 TNTN 77
          T  N
Sbjct: 61 TFEN 63

The following BLAST results are available for this feature:
BLAST of Lsi02G004490 vs. Swiss-Prot
Match NameE-valueIdentityDescription
BLAST of Lsi02G004490 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LR14_CUCSA1.0e-0960.38Pectinesterase OS=Cucumis sativus GN=Csa_1G049450 PE=4 SV=1[more]
A0A0A0KNI2_CUCSA2.8e-0761.11Pectinesterase OS=Cucumis sativus GN=Csa_5G488760 PE=4 SV=1[more]
W9R0V6_9ROSA3.1e-0653.85Pectinesterase OS=Morus notabilis GN=L484_012499 PE=4 SV=1[more]
W9QFB9_9ROSA4.1e-0653.85Pectinesterase OS=Morus notabilis GN=L484_012492 PE=4 SV=1[more]
A0A0A0L1M3_CUCSA5.3e-0656.60Pectinesterase OS=Cucumis sativus GN=Csa_4G260430 PE=4 SV=1[more]
BLAST of Lsi02G004490 vs. TAIR10
Match NameE-valueIdentityDescription
BLAST of Lsi02G004490 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659068777|ref|XP_008446175.1|1.8e-1569.57PREDICTED: pectinesterase 2-like [Cucumis melo][more]
gi|659068775|ref|XP_008446163.1|4.0e-1569.57PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis melo][more]
gi|778708428|ref|XP_011656190.1|2.9e-1365.22PREDICTED: probable pectinesterase/pectinesterase inhibitor 41 [Cucumis sativus][more]
gi|778657958|ref|XP_004152582.2|1.5e-0960.38PREDICTED: pectinesterase 2-like [Cucumis sativus][more]
gi|659131264|ref|XP_008465596.1|1.3e-0856.25PREDICTED: probable pectinesterase/pectinesterase inhibitor 47, partial [Cucumis... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: INTERPRO
TermDefinition
IPR012334Pectin_lyas_fold
IPR011050Pectin_lyase_fold/virulence
IPR000070Pectinesterase_cat
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
cellular_component GO:0005618 cell wall
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G004490.1Lsi02G004490.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 8..74
score: 6.
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 7..76
score: 2.
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 7..61
score: 3.3

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi02G004490Cucumber (Gy14) v2cgyblsiB018
Lsi02G004490Melon (DHL92) v3.6.1lsimedB189
Lsi02G004490Silver-seed gourdcarlsiB447
Lsi02G004490Silver-seed gourdcarlsiB918
Lsi02G004490Cucumber (Chinese Long) v3cuclsiB022
Lsi02G004490Watermelon (97103) v2lsiwmbB176
Lsi02G004490Wax gourdlsiwgoB240
Lsi02G004490Cucumber (Gy14) v1cgylsiB633
Lsi02G004490Cucurbita maxima (Rimu)cmalsiB202
Lsi02G004490Cucurbita maxima (Rimu)cmalsiB370
Lsi02G004490Cucurbita moschata (Rifu)cmolsiB192
Lsi02G004490Cucurbita moschata (Rifu)cmolsiB356
Lsi02G004490Cucurbita pepo (Zucchini)cpelsiB026
Lsi02G004490Cucurbita pepo (Zucchini)cpelsiB423
Lsi02G004490Wild cucumber (PI 183967)cpilsiB021
Lsi02G004490Cucumber (Chinese Long) v2culsiB022
Lsi02G004490Melon (DHL92) v3.5.1lsimeB179
Lsi02G004490Watermelon (Charleston Gray)lsiwcgB184
Lsi02G004490Watermelon (97103) v1lsiwmB184