Lsi02G002520 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi02G002520
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Descriptionreceptor serine/threonine kinase, putative
Locationchr02 : 2139037 .. 2139993 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTATCGTCCGTGATCGCCGCCACTTAAACCTCCGCCTTCCCGACCTCTCCGACTGCCGTCCCCGCTTCCCTCTCCCCCTTCCTCCCTCCTCTGCCCCCGCCCCTCCCGCCGCCCCCTCCGCCATCTCCTCCTCCGACCTCGACAAGCTCCAAGTCCTCGGCCACGGCAACGGCGGCACCGTCTACAAAGTCCGCCACAAACGCACCTCCACCACTTACGCTCTCAAAGTCGTCCATGGCGACTGCGATCCCACCGTCCGTCGCCAAGTTTTCAGAGAGATGGAAATTCTCCGCCGTACTGATTCTCCCTATGTCGTTCAGTGCCATGGAATCTTCGAGAAGCCTTCTGGAGATGTAACGATTTTGATGGAGTATATGGATCTAGGATCGCTTGATTCGCTCTTGAAAAAGAATTCGACGTTGTCAGAAGCGACGCTCGCTCACGTATCGCGTCAAGTTCTTAACGGACTTCATTATCTTCATTCTCACAAAATCATTCATCGCGATATTAAACCGTCGAATCTGTTGGTGAATAAGAATATGGAGGTTAAGATTGCCGATTTTGGAGTTAGTAAAATTATGTGCAGGACTTTGGATGCTTGCAATTCCTACGTCGGAACTTGCGCTTATATGAGTCCGGAGCGGTTCGATCCAGAGACTTACGGCGGAAATTACAACGGTTACGCCGGCGATATTTGGAGTTTGGGGCTTACTCTTCTGGAACTTTATTTAGGTCATTTTCCTTTTCTTCCGCCTGGACAGAGACCGGATTGGGCGACTCTGATGTGTGCGATTTGCTTCGGTGAACCACCGACGCTGCCGGAGAATGCGTCGGAGGAGTTTCGGAGCTTTGTTGAGTGTTGCTTGCAGAAGGAATCGAGCAAGAGGTGGACGGCGGCGCAATTGTTAACGCATCCGTTTGTATGCAGGGAATCATCAAGGTCCGACCGATGA

mRNA sequence

ATGGCTATCGTCCGTGATCGCCGCCACTTAAACCTCCGCCTTCCCGACCTCTCCGACTGCCGTCCCCGCTTCCCTCTCCCCCTTCCTCCCTCCTCTGCCCCCGCCCCTCCCGCCGCCCCCTCCGCCATCTCCTCCTCCGACCTCGACAAGCTCCAAGTCCTCGGCCACGGCAACGGCGGCACCGTCTACAAAGTCCGCCACAAACGCACCTCCACCACTTACGCTCTCAAAGTCGTCCATGGCGACTGCGATCCCACCGTCCGTCGCCAAGTTTTCAGAGAGATGGAAATTCTCCGCCGTACTGATTCTCCCTATGTCGTTCAGTGCCATGGAATCTTCGAGAAGCCTTCTGGAGATGTAACGATTTTGATGGAGTATATGGATCTAGGATCGCTTGATTCGCTCTTGAAAAAGAATTCGACGTTGTCAGAAGCGACGCTCGCTCACGTATCGCGTCAAGTTCTTAACGGACTTCATTATCTTCATTCTCACAAAATCATTCATCGCGATATTAAACCGTCGAATCTGTTGGTGAATAAGAATATGGAGGTTAAGATTGCCGATTTTGGAGTTAGTAAAATTATGTGCAGGACTTTGGATGCTTGCAATTCCTACGTCGGAACTTGCGCTTATATGAGTCCGGAGCGGTTCGATCCAGAGACTTACGGCGGAAATTACAACGGTTACGCCGGCGATATTTGGAGTTTGGGGCTTACTCTTCTGGAACTTTATTTAGGTCATTTTCCTTTTCTTCCGCCTGGACAGAGACCGGATTGGGCGACTCTGATGTGTGCGATTTGCTTCGGTGAACCACCGACGCTGCCGGAGAATGCGTCGGAGGAGTTTCGGAGCTTTGTTGAGTGTTGCTTGCAGAAGGAATCGAGCAAGAGGTGGACGGCGGCGCAATTGTTAACGCATCCGTTTGTATGCAGGGAATCATCAAGGTCCGACCGATGA

Coding sequence (CDS)

ATGGCTATCGTCCGTGATCGCCGCCACTTAAACCTCCGCCTTCCCGACCTCTCCGACTGCCGTCCCCGCTTCCCTCTCCCCCTTCCTCCCTCCTCTGCCCCCGCCCCTCCCGCCGCCCCCTCCGCCATCTCCTCCTCCGACCTCGACAAGCTCCAAGTCCTCGGCCACGGCAACGGCGGCACCGTCTACAAAGTCCGCCACAAACGCACCTCCACCACTTACGCTCTCAAAGTCGTCCATGGCGACTGCGATCCCACCGTCCGTCGCCAAGTTTTCAGAGAGATGGAAATTCTCCGCCGTACTGATTCTCCCTATGTCGTTCAGTGCCATGGAATCTTCGAGAAGCCTTCTGGAGATGTAACGATTTTGATGGAGTATATGGATCTAGGATCGCTTGATTCGCTCTTGAAAAAGAATTCGACGTTGTCAGAAGCGACGCTCGCTCACGTATCGCGTCAAGTTCTTAACGGACTTCATTATCTTCATTCTCACAAAATCATTCATCGCGATATTAAACCGTCGAATCTGTTGGTGAATAAGAATATGGAGGTTAAGATTGCCGATTTTGGAGTTAGTAAAATTATGTGCAGGACTTTGGATGCTTGCAATTCCTACGTCGGAACTTGCGCTTATATGAGTCCGGAGCGGTTCGATCCAGAGACTTACGGCGGAAATTACAACGGTTACGCCGGCGATATTTGGAGTTTGGGGCTTACTCTTCTGGAACTTTATTTAGGTCATTTTCCTTTTCTTCCGCCTGGACAGAGACCGGATTGGGCGACTCTGATGTGTGCGATTTGCTTCGGTGAACCACCGACGCTGCCGGAGAATGCGTCGGAGGAGTTTCGGAGCTTTGTTGAGTGTTGCTTGCAGAAGGAATCGAGCAAGAGGTGGACGGCGGCGCAATTGTTAACGCATCCGTTTGTATGCAGGGAATCATCAAGGTCCGACCGATGA

Protein sequence

MAIVRDRRHLNLRLPDLSDCRPRFPLPLPPSSAPAPPAAPSAISSSDLDKLQVLGHGNGGTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDVTILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPENASEEFRSFVECCLQKESSKRWTAAQLLTHPFVCRESSRSDR
Homology
[Close]
BLAST of Lsi02G002520 vs. Swiss-Prot
Match: M2K9_ARATH (Mitogen-activated protein kinase kinase 9 OS=Arabidopsis thaliana GN=MKK9 PE=1 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 1.6e-113
Identity = 207/310 (66.77%), Postives = 241/310 (77.74%), Query Frame = 1 

Query: 1   MAIVRDRRHLNLRLPDLSDCRPRFPLPLPPSSAPAPPAAPSAISSSDLDKLQVLGHGNGG 60
           MA+VR+RR LNLRLP       RF      S+     A  + IS+ DL+KL VLG GNGG
Sbjct: 1   MALVRERRQLNLRLPLPPISDRRFSTS-SSSATTTTVAGCNGISACDLEKLNVLGCGNGG 60

Query: 61  TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPS-GD 120
            VYKVRHK TS  YALK V+GD DP   RQ+ REMEILRRTDSPYVV+CHGIFEKP  G+
Sbjct: 61  IVYKVRHKTTSEIYALKTVNGDMDPIFTRQLMREMEILRRTDSPYVVKCHGIFEKPVVGE 120

Query: 121 VTILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVN 180
           V+ILMEYMD G+L+SL      ++E  LA  ++Q+L GL YLH+ KI+HRDIKP+NLL+N
Sbjct: 121 VSILMEYMDGGTLESL---RGGVTEQKLAGFAKQILKGLSYLHALKIVHRDIKPANLLLN 180

Query: 181 KNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLT 240
              EVKIADFGVSKI+ R+LD+CNSYVGTCAYMSPERFD E+ GG+ + YAGDIWS GL 
Sbjct: 181 SKNEVKIADFGVSKILVRSLDSCNSYVGTCAYMSPERFDSESSGGSSDIYAGDIWSFGLM 240

Query: 241 LLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPENASEEFRSFVECCLQKESSKRWT 300
           +LEL +GHFP LPPGQRPDWATLMCA+CFGEPP  PE  SEEFRSFVECCL+K+SSKRWT
Sbjct: 241 MLELLVGHFPLLPPGQRPDWATLMCAVCFGEPPRAPEGCSEEFRSFVECCLRKDSSKRWT 300

Query: 301 AAQLLTHPFV 310
           A QLL HPF+
Sbjct: 301 APQLLAHPFL 306

[Close]
BLAST of Lsi02G002520 vs. Swiss-Prot
Match: M2K7_ARATH (Mitogen-activated protein kinase kinase 7 OS=Arabidopsis thaliana GN=MKK7 PE=1 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 3.1e-106
Identity = 201/316 (63.61%), Postives = 237/316 (75.00%), Query Frame = 1 

Query: 1   MAIVRDRRHLNLRLPDLSDCRPRFPLPLPPSS--APAPPAAPSAISSSDLDKLQVLGHGN 60
           MA+VR RR +NLRLP      P   + LP  S  +   P   + IS+SD++KL VLG G+
Sbjct: 1   MALVRKRRQINLRLPV-----PPLSVHLPWFSFASSTAPVINNGISASDVEKLHVLGRGS 60

Query: 61  GGTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPS- 120
            G VYKV HK T   YALK V+GD  P   RQ+ REMEILRRTDSPYVV+C GIFEKP  
Sbjct: 61  SGIVYKVHHKTTGEIYALKSVNGDMSPAFTRQLAREMEILRRTDSPYVVRCQGIFEKPIV 120

Query: 121 GDVTILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLL 180
           G+V+ILMEYMD G+L+SL      ++E  LA  SRQ+L GL YLHS KI+HRDIKP+NLL
Sbjct: 121 GEVSILMEYMDGGNLESL---RGAVTEKQLAGFSRQILKGLSYLHSLKIVHRDIKPANLL 180

Query: 181 VNKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLG 240
           +N   EVKIADFGVSKI+ R+LD CNSYVGTCAYMSPERFD    G N + YAGDIWS G
Sbjct: 181 LNSRNEVKIADFGVSKIITRSLDYCNSYVGTCAYMSPERFDSAA-GENSDVYAGDIWSFG 240

Query: 241 LTLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPENASEEFRSFVECCLQKESSKR 300
           + +LEL++GHFP LP GQRPDWATLMC +CFGEPP  PE  S+EFRSFV+CCL+KESS+R
Sbjct: 241 VMILELFVGHFPLLPQGQRPDWATLMCVVCFGEPPRAPEGCSDEFRSFVDCCLRKESSER 300

Query: 301 WTAAQLLTHPFVCRES 314
           WTA+QLL HPF+ RES
Sbjct: 301 WTASQLLGHPFL-RES 306

[Close]
BLAST of Lsi02G002520 vs. Swiss-Prot
Match: M2K5_ARATH (Mitogen-activated protein kinase kinase 5 OS=Arabidopsis thaliana GN=MKK5 PE=1 SV=2)

HSP 1 Score: 322.8 bits (826), Expect = 4.3e-87
Identity = 167/317 (52.68%), Postives = 220/317 (69.40%), Query Frame = 1 

Query: 4   VRDRRHLNLRLPDLSDCRPRFPLPLPP-SSAPAPPAAPSAISS--------SDLDKLQVL 63
           +R R  L+L LP   D     PLPLPP SS+ + PA+ SAIS+        S+L+++  +
Sbjct: 18  LRKRPDLSLPLPH-RDVALAVPLPLPPPSSSSSAPASSSAISTNISAAKSLSELERVNRI 77

Query: 64  GHGNGGTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFE 123
           G G GGTVYKV H  TS  +ALKV++G+ + TVRRQ+ RE+EILR  D P VV+CH +F+
Sbjct: 78  GSGAGGTVYKVIHTPTSRPFALKVIYGNHEDTVRRQICREIEILRSVDHPNVVKCHDMFD 137

Query: 124 KPSGDVTILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPS 183
             +G++ +L+E+MD GSL+          E  LA +SRQ+L+GL YLH   I+HRDIKPS
Sbjct: 138 H-NGEIQVLLEFMDQGSLEGA----HIWQEQELADLSRQILSGLAYLHRRHIVHRDIKPS 197

Query: 184 NLLVNKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIW 243
           NLL+N    VKIADFGVS+I+ +T+D CNS VGT AYMSPER + +   G Y+GYAGD+W
Sbjct: 198 NLLINSAKNVKIADFGVSRILAQTMDPCNSSVGTIAYMSPERINTDLNHGRYDGYAGDVW 257

Query: 244 SLGLTLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPENASEEFRSFVECCLQKES 303
           SLG+++LE YLG FPF    ++ DWA+LMCAIC  +PP  P  AS+EFR FV CCLQ + 
Sbjct: 258 SLGVSILEFYLGRFPF-AVSRQGDWASLMCAICMSQPPEAPATASQEFRHFVSCCLQSDP 317

Query: 304 SKRWTAAQLLTHPFVCR 312
            KRW+A QLL HPF+ +
Sbjct: 318 PKRWSAQQLLQHPFILK 327

[Close]
BLAST of Lsi02G002520 vs. Swiss-Prot
Match: M2K8_ARATH (Mitogen-activated protein kinase kinase 8 OS=Arabidopsis thaliana GN=MKK8 PE=3 SV=2)

HSP 1 Score: 322.0 bits (824), Expect = 7.3e-87
Identity = 170/321 (52.96%), Postives = 222/321 (69.16%), Query Frame = 1 

Query: 1   MAIVRDRRHLNLRLPDLSDCRP----RFPL-PLPPSSAPAPPAAPSAISSSDLDKLQVLG 60
           M +VRD + LNL+L  +         RFP+ P    SA     A +  S ++LD++ VLG
Sbjct: 1   MVMVRDNQFLNLKLSPIQAPTTIPPCRFPIIPATKVSATVSSCASNTFSVANLDRISVLG 60

Query: 61  HGNGGTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEK 120
            GNGGTV+KV+ K TS  YALK V  + D T      RE+EILR  +SPYV +CH IF+ 
Sbjct: 61  SGNGGTVFKVKDKTTSEIYALKKVKENWDSTS----LREIEILRMVNSPYVAKCHDIFQN 120

Query: 121 PSGDVTILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSN 180
           PSG+V+ILM+YMDLGSL+SL      ++E  LA +SRQVL G +YLH HKI+HRDIKP+N
Sbjct: 121 PSGEVSILMDYMDLGSLESL----RGVTEKQLALMSRQVLEGKNYLHEHKIVHRDIKPAN 180

Query: 181 LLVNKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYG----GNYNGYAG 240
           LL +   EVKIADFGVSKI+ R+L+ CNS+VGT AYMSPER D E  G       N YAG
Sbjct: 181 LLRSSKEEVKIADFGVSKIVVRSLNKCNSFVGTFAYMSPERLDSEADGVTEEDKSNVYAG 240

Query: 241 DIWSLGLTLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPENASEEFRSFVECCLQ 300
           DIWS GLT+LE+ +G++P L     PD A ++CA+CFGEPP  PE  S++ +SF++CCL+
Sbjct: 241 DIWSFGLTMLEILVGYYPML-----PDQAAIVCAVCFGEPPKAPEECSDDLKSFMDCCLR 300

Query: 301 KESSKRWTAAQLLTHPFVCRE 313
           K++S+RWTA+QLL HPF+  +
Sbjct: 301 KKASERWTASQLLNHPFLLHQ 308

[Close]
BLAST of Lsi02G002520 vs. Swiss-Prot
Match: M2K4_ARATH (Mitogen-activated protein kinase kinase 4 OS=Arabidopsis thaliana GN=MKK4 PE=1 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 2.3e-85
Identity = 171/333 (51.35%), Postives = 220/333 (66.07%), Query Frame = 1 

Query: 5   RDRRHLNLRLP-DLSDCRPRFPLPLPP-------SSAPAPPAAPSAISS----------- 64
           R RR  +L LP    D     PLPLPP       SS  AP +  SA S+           
Sbjct: 17  RPRRRPDLTLPLPQRDVSLAVPLPLPPTSGGSGGSSGSAPSSGGSASSTNTNSSIEAKNY 76

Query: 65  SDLDKLQVLGHGNGGTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPY 124
           SDL +   +G G GGTVYKV H+ +S  YALKV++G+ + TVRRQ+ RE+EILR  + P 
Sbjct: 77  SDLVRGNRIGSGAGGTVYKVIHRPSSRLYALKVIYGNHEETVRRQICREIEILRDVNHPN 136

Query: 125 VVQCHGIFEKPSGDVTILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHK 184
           VV+CH +F++ +G++ +L+E+MD GSL+          E  LA +SRQ+L+GL YLHS  
Sbjct: 137 VVKCHEMFDQ-NGEIQVLLEFMDKGSLEGA----HVWKEQQLADLSRQILSGLAYLHSRH 196

Query: 185 IIHRDIKPSNLLVNKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGN 244
           I+HRDIKPSNLL+N    VKIADFGVS+I+ +T+D CNS VGT AYMSPER + +   G 
Sbjct: 197 IVHRDIKPSNLLINSAKNVKIADFGVSRILAQTMDPCNSSVGTIAYMSPERINTDLNQGK 256

Query: 245 YNGYAGDIWSLGLTLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPENASEEFRSF 304
           Y+GYAGDIWSLG+++LE YLG FPF P  ++ DWA+LMCAIC  +PP  P  AS EFR F
Sbjct: 257 YDGYAGDIWSLGVSILEFYLGRFPF-PVSRQGDWASLMCAICMSQPPEAPATASPEFRHF 316

Query: 305 VECCLQKESSKRWTAAQLLTHPFVCRESSRSDR 319
           + CCLQ+E  KR +A QLL HPF+ R S   +R
Sbjct: 317 ISCCLQREPGKRRSAMQLLQHPFILRASPSQNR 343

[Close]
BLAST of Lsi02G002520 vs. TrEMBL
Match: A0A0A0LQY3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G042980 PE=3 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 1.2e-181
Identity = 310/316 (98.10%), Postives = 312/316 (98.73%), Query Frame = 1 

Query: 1   MAIVRDRRHLNLRLPDLSDCRPRFPLPLPPSSAPAPPAAPSAISSSDLDKLQVLGHGNGG 60
           MA+VRDRRHLNLRLPDLSDCRPRFPLPLPPSSAP  PAAPSAISSSDLDKLQVLGHGNGG
Sbjct: 1   MALVRDRRHLNLRLPDLSDCRPRFPLPLPPSSAPPAPAAPSAISSSDLDKLQVLGHGNGG 60

Query: 61  TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV 120
           TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV
Sbjct: 61  TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV 120

Query: 121 TILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180
           TILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK
Sbjct: 121 TILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180

Query: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL 240
           NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL
Sbjct: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL 240

Query: 241 LELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPENASEEFRSFVECCLQKESSKRWTA 300
           LELYLGHFPFLP GQRPDWATLMCAICFGEPP LPE+ASEEFRSFVECCLQKESSKRWTA
Sbjct: 241 LELYLGHFPFLPAGQRPDWATLMCAICFGEPPKLPEDASEEFRSFVECCLQKESSKRWTA 300

Query: 301 AQLLTHPFVCRESSRS 317
           AQLLTHPFVCRESSRS
Sbjct: 301 AQLLTHPFVCRESSRS 316

[Close]
BLAST of Lsi02G002520 vs. TrEMBL
Match: A0A0U5PS50_CUCSA (MAP kinase Kinase 4 OS=Cucumis sativus GN=MKK4 PE=3 SV=1)

HSP 1 Score: 638.6 bits (1646), Expect = 3.9e-180
Identity = 308/316 (97.47%), Postives = 310/316 (98.10%), Query Frame = 1 

Query: 1   MAIVRDRRHLNLRLPDLSDCRPRFPLPLPPSSAPAPPAAPSAISSSDLDKLQVLGHGNGG 60
           MA+VRDRRHLNLRLPDLSDCRPRF LPLPPSSAP  PAAPSAISSSDLDKLQVLGHGNGG
Sbjct: 1   MALVRDRRHLNLRLPDLSDCRPRFSLPLPPSSAPPAPAAPSAISSSDLDKLQVLGHGNGG 60

Query: 61  TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV 120
           TVYKVRHKRTS TYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV
Sbjct: 61  TVYKVRHKRTSATYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV 120

Query: 121 TILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180
           TILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK
Sbjct: 121 TILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180

Query: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL 240
           NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL
Sbjct: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL 240

Query: 241 LELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPENASEEFRSFVECCLQKESSKRWTA 300
           LELYLGHFPFLP GQRPDWATLMCAICFGEPP LPE+ASEEFRSFVECCLQKESSKRWTA
Sbjct: 241 LELYLGHFPFLPAGQRPDWATLMCAICFGEPPKLPEDASEEFRSFVECCLQKESSKRWTA 300

Query: 301 AQLLTHPFVCRESSRS 317
           AQLLTHPFVCRESSRS
Sbjct: 301 AQLLTHPFVCRESSRS 316

[Close]
BLAST of Lsi02G002520 vs. TrEMBL
Match: W9QZB3_9ROSA (Mitogen-activated protein kinase kinase 4 OS=Morus notabilis GN=L484_019336 PE=3 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 9.7e-147
Identity = 249/326 (76.38%), Postives = 287/326 (88.04%), Query Frame = 1 

Query: 1   MAIVRDRRHLNLRLP--DLSDCRPRFPLPLPPSSAPAPP-----------AAPSAISSSD 60
           MA+VR RR LNLRLP  ++S+ RPRFPLPLPPS+A +             A+ + IS++D
Sbjct: 1   MAVVRGRRQLNLRLPLPEISERRPRFPLPLPPSAATSDTNHNSNNNNWLSASSADISAAD 60

Query: 61  LDKLQVLGHGNGGTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVV 120
           ++KL VLGHGNGGTVYKVRHK+TS TYALK++HGD D TVRRQ+ REMEILRRTDSP+VV
Sbjct: 61  VEKLNVLGHGNGGTVYKVRHKKTSLTYALKLIHGDSDSTVRRQLLREMEILRRTDSPHVV 120

Query: 121 QCHGIFEKPSGDVTILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKII 180
            CHG+FEKPSGD+ ILMEYMD G+L+++LK + T SE  LA+V+RQVLNGL+YLHSHKII
Sbjct: 121 HCHGMFEKPSGDIAILMEYMDFGTLETILKADGTFSEPKLAYVARQVLNGLNYLHSHKII 180

Query: 181 HRDIKPSNLLVNKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYN 240
           HRDIKPSNLLVN+NME+KIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNY+
Sbjct: 181 HRDIKPSNLLVNRNMEIKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYD 240

Query: 241 GYAGDIWSLGLTLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPENASEEFRSFVE 300
           GYAGDIWSLGLTL+ELYLGHFP LP GQRPDWATLMCAICFGEPPTLP+  SEEFRSF+E
Sbjct: 241 GYAGDIWSLGLTLMELYLGHFPLLPAGQRPDWATLMCAICFGEPPTLPDGVSEEFRSFIE 300

Query: 301 CCLQKESSKRWTAAQLLTHPFVCRES 314
           CCLQKESSKRW+ +QLL+HPF+CR+S
Sbjct: 301 CCLQKESSKRWSTSQLLSHPFLCRDS 326

[Close]
BLAST of Lsi02G002520 vs. TrEMBL
Match: B9I4T2_POPTR (Mitogen-activated protein kinase kinase OS=Populus trichocarpa GN=POPTR_0012s04010g PE=3 SV=2)

HSP 1 Score: 525.0 bits (1351), Expect = 6.3e-146
Identity = 244/311 (78.46%), Postives = 276/311 (88.75%), Query Frame = 1 

Query: 1   MAIVRDRRHLNLRLPDLSDCRPRFPLPLPPSSAPAPPAAPSAISSSDLDKLQVLGHGNGG 60
           MA+VR+RR +NLRLP+LSD RPRFPLPLPP+S        S IS +D++K+ VLGHGNGG
Sbjct: 1   MALVRERRQVNLRLPELSDRRPRFPLPLPPTSTINNNNTTSTISCNDIEKIHVLGHGNGG 60

Query: 61  TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV 120
           TVYKVRHKR S  YALKVVHGD DP VRRQ++RE+EILRRTDSP +V+CHG++EKPSGD+
Sbjct: 61  TVYKVRHKRNSQIYALKVVHGDSDPLVRRQIYREIEILRRTDSPNIVKCHGVYEKPSGDI 120

Query: 121 TILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180
            I MEYMDLG+LDSLL+K+ T +E+ L+HV+ QVLNGL YLH+ KIIHRDIKPSNLLVNK
Sbjct: 121 AITMEYMDLGTLDSLLQKHGTFNESKLSHVASQVLNGLSYLHAQKIIHRDIKPSNLLVNK 180

Query: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL 240
           +MEVKIADFGVSKIM RTLDACNSYVGTCAYMSPERFDP+TYGGNYNGYA DIWSLGL L
Sbjct: 181 DMEVKIADFGVSKIMHRTLDACNSYVGTCAYMSPERFDPDTYGGNYNGYAADIWSLGLIL 240

Query: 241 LELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPENASEEFRSFVECCLQKESSKRWTA 300
           LELYLGHFPFLPPGQRPDWATLMCAICFG+PP+LPE ASEEFR F++CCLQKES KRWTA
Sbjct: 241 LELYLGHFPFLPPGQRPDWATLMCAICFGDPPSLPEGASEEFRDFIQCCLQKESGKRWTA 300

Query: 301 AQLLTHPFVCR 312
           AQLL HPF C+
Sbjct: 301 AQLLAHPFACK 311

[Close]
BLAST of Lsi02G002520 vs. TrEMBL
Match: A9PJT7_9ROSI (Putative uncharacterized protein OS=Populus trichocarpa x Populus deltoides PE=2 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 4.1e-145
Identity = 245/312 (78.53%), Postives = 277/312 (88.78%), Query Frame = 1 

Query: 1   MAIVRDRRHLNLRLPDLSDCRPRFPLPLPPSSA-PAPPAAPSAISSSDLDKLQVLGHGNG 60
           MA+VR+RR +NLRLP+LSD RPRFPLPLPP+S         S IS +D++K+ VLGHGNG
Sbjct: 1   MALVRERRQVNLRLPELSDRRPRFPLPLPPTSTINNNNNNTSTISCNDIEKIHVLGHGNG 60

Query: 61  GTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGD 120
           GTVYKVRHKR S  YALKVVHGD DP VRRQ++RE+EILRRTDSP +V+CHG++EKPSGD
Sbjct: 61  GTVYKVRHKRNSQIYALKVVHGDSDPLVRRQIYREIEILRRTDSPNIVKCHGVYEKPSGD 120

Query: 121 VTILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVN 180
           + I MEYMDLG+LDSLLKK+ T +E+ L+HV+ QVLNGL YLH+ KIIHRDIKPSNLLVN
Sbjct: 121 IAITMEYMDLGTLDSLLKKHGTFNESKLSHVASQVLNGLSYLHAQKIIHRDIKPSNLLVN 180

Query: 181 KNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLT 240
           K+MEVKIADFGVSKIM RTLDACNSYVGTCAYMSPERFDP+TYGGNYNGYA DIWSLGL 
Sbjct: 181 KDMEVKIADFGVSKIMHRTLDACNSYVGTCAYMSPERFDPDTYGGNYNGYAADIWSLGLI 240

Query: 241 LLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPENASEEFRSFVECCLQKESSKRWT 300
           LLELY+GHFPFLPPGQRPDWATLMCAICFG+PP+LPE ASEEFR F++CCLQKES KRWT
Sbjct: 241 LLELYMGHFPFLPPGQRPDWATLMCAICFGDPPSLPEGASEEFRDFIQCCLQKESGKRWT 300

Query: 301 AAQLLTHPFVCR 312
           AAQLL HPFVC+
Sbjct: 301 AAQLLAHPFVCK 312

[Close]
BLAST of Lsi02G002520 vs. TAIR10
Match: AT1G73500.1 (AT1G73500.1 MAP kinase kinase 9)

HSP 1 Score: 410.6 bits (1054), Expect = 8.8e-115
Identity = 207/310 (66.77%), Postives = 241/310 (77.74%), Query Frame = 1 

Query: 1   MAIVRDRRHLNLRLPDLSDCRPRFPLPLPPSSAPAPPAAPSAISSSDLDKLQVLGHGNGG 60
           MA+VR+RR LNLRLP       RF      S+     A  + IS+ DL+KL VLG GNGG
Sbjct: 1   MALVRERRQLNLRLPLPPISDRRFSTS-SSSATTTTVAGCNGISACDLEKLNVLGCGNGG 60

Query: 61  TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPS-GD 120
            VYKVRHK TS  YALK V+GD DP   RQ+ REMEILRRTDSPYVV+CHGIFEKP  G+
Sbjct: 61  IVYKVRHKTTSEIYALKTVNGDMDPIFTRQLMREMEILRRTDSPYVVKCHGIFEKPVVGE 120

Query: 121 VTILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVN 180
           V+ILMEYMD G+L+SL      ++E  LA  ++Q+L GL YLH+ KI+HRDIKP+NLL+N
Sbjct: 121 VSILMEYMDGGTLESL---RGGVTEQKLAGFAKQILKGLSYLHALKIVHRDIKPANLLLN 180

Query: 181 KNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLT 240
              EVKIADFGVSKI+ R+LD+CNSYVGTCAYMSPERFD E+ GG+ + YAGDIWS GL 
Sbjct: 181 SKNEVKIADFGVSKILVRSLDSCNSYVGTCAYMSPERFDSESSGGSSDIYAGDIWSFGLM 240

Query: 241 LLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPENASEEFRSFVECCLQKESSKRWT 300
           +LEL +GHFP LPPGQRPDWATLMCA+CFGEPP  PE  SEEFRSFVECCL+K+SSKRWT
Sbjct: 241 MLELLVGHFPLLPPGQRPDWATLMCAVCFGEPPRAPEGCSEEFRSFVECCLRKDSSKRWT 300

Query: 301 AAQLLTHPFV 310
           A QLL HPF+
Sbjct: 301 APQLLAHPFL 306

[Close]
BLAST of Lsi02G002520 vs. TAIR10
Match: AT1G18350.1 (AT1G18350.1 MAP kinase kinase 7)

HSP 1 Score: 386.3 bits (991), Expect = 1.8e-107
Identity = 201/316 (63.61%), Postives = 237/316 (75.00%), Query Frame = 1 

Query: 1   MAIVRDRRHLNLRLPDLSDCRPRFPLPLPPSS--APAPPAAPSAISSSDLDKLQVLGHGN 60
           MA+VR RR +NLRLP      P   + LP  S  +   P   + IS+SD++KL VLG G+
Sbjct: 1   MALVRKRRQINLRLPV-----PPLSVHLPWFSFASSTAPVINNGISASDVEKLHVLGRGS 60

Query: 61  GGTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPS- 120
            G VYKV HK T   YALK V+GD  P   RQ+ REMEILRRTDSPYVV+C GIFEKP  
Sbjct: 61  SGIVYKVHHKTTGEIYALKSVNGDMSPAFTRQLAREMEILRRTDSPYVVRCQGIFEKPIV 120

Query: 121 GDVTILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLL 180
           G+V+ILMEYMD G+L+SL      ++E  LA  SRQ+L GL YLHS KI+HRDIKP+NLL
Sbjct: 121 GEVSILMEYMDGGNLESL---RGAVTEKQLAGFSRQILKGLSYLHSLKIVHRDIKPANLL 180

Query: 181 VNKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLG 240
           +N   EVKIADFGVSKI+ R+LD CNSYVGTCAYMSPERFD    G N + YAGDIWS G
Sbjct: 181 LNSRNEVKIADFGVSKIITRSLDYCNSYVGTCAYMSPERFDSAA-GENSDVYAGDIWSFG 240

Query: 241 LTLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPENASEEFRSFVECCLQKESSKR 300
           + +LEL++GHFP LP GQRPDWATLMC +CFGEPP  PE  S+EFRSFV+CCL+KESS+R
Sbjct: 241 VMILELFVGHFPLLPQGQRPDWATLMCVVCFGEPPRAPEGCSDEFRSFVDCCLRKESSER 300

Query: 301 WTAAQLLTHPFVCRES 314
           WTA+QLL HPF+ RES
Sbjct: 301 WTASQLLGHPFL-RES 306

[Close]
BLAST of Lsi02G002520 vs. TAIR10
Match: AT3G21220.1 (AT3G21220.1 MAP kinase kinase 5)

HSP 1 Score: 322.8 bits (826), Expect = 2.4e-88
Identity = 167/317 (52.68%), Postives = 220/317 (69.40%), Query Frame = 1 

Query: 4   VRDRRHLNLRLPDLSDCRPRFPLPLPP-SSAPAPPAAPSAISS--------SDLDKLQVL 63
           +R R  L+L LP   D     PLPLPP SS+ + PA+ SAIS+        S+L+++  +
Sbjct: 18  LRKRPDLSLPLPH-RDVALAVPLPLPPPSSSSSAPASSSAISTNISAAKSLSELERVNRI 77

Query: 64  GHGNGGTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFE 123
           G G GGTVYKV H  TS  +ALKV++G+ + TVRRQ+ RE+EILR  D P VV+CH +F+
Sbjct: 78  GSGAGGTVYKVIHTPTSRPFALKVIYGNHEDTVRRQICREIEILRSVDHPNVVKCHDMFD 137

Query: 124 KPSGDVTILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPS 183
             +G++ +L+E+MD GSL+          E  LA +SRQ+L+GL YLH   I+HRDIKPS
Sbjct: 138 H-NGEIQVLLEFMDQGSLEGA----HIWQEQELADLSRQILSGLAYLHRRHIVHRDIKPS 197

Query: 184 NLLVNKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIW 243
           NLL+N    VKIADFGVS+I+ +T+D CNS VGT AYMSPER + +   G Y+GYAGD+W
Sbjct: 198 NLLINSAKNVKIADFGVSRILAQTMDPCNSSVGTIAYMSPERINTDLNHGRYDGYAGDVW 257

Query: 244 SLGLTLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPENASEEFRSFVECCLQKES 303
           SLG+++LE YLG FPF    ++ DWA+LMCAIC  +PP  P  AS+EFR FV CCLQ + 
Sbjct: 258 SLGVSILEFYLGRFPF-AVSRQGDWASLMCAICMSQPPEAPATASQEFRHFVSCCLQSDP 317

Query: 304 SKRWTAAQLLTHPFVCR 312
            KRW+A QLL HPF+ +
Sbjct: 318 PKRWSAQQLLQHPFILK 327

[Close]
BLAST of Lsi02G002520 vs. TAIR10
Match: AT1G51660.1 (AT1G51660.1 mitogen-activated protein kinase kinase 4)

HSP 1 Score: 317.0 bits (811), Expect = 1.3e-86
Identity = 171/333 (51.35%), Postives = 220/333 (66.07%), Query Frame = 1 

Query: 5   RDRRHLNLRLP-DLSDCRPRFPLPLPP-------SSAPAPPAAPSAISS----------- 64
           R RR  +L LP    D     PLPLPP       SS  AP +  SA S+           
Sbjct: 17  RPRRRPDLTLPLPQRDVSLAVPLPLPPTSGGSGGSSGSAPSSGGSASSTNTNSSIEAKNY 76

Query: 65  SDLDKLQVLGHGNGGTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPY 124
           SDL +   +G G GGTVYKV H+ +S  YALKV++G+ + TVRRQ+ RE+EILR  + P 
Sbjct: 77  SDLVRGNRIGSGAGGTVYKVIHRPSSRLYALKVIYGNHEETVRRQICREIEILRDVNHPN 136

Query: 125 VVQCHGIFEKPSGDVTILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHK 184
           VV+CH +F++ +G++ +L+E+MD GSL+          E  LA +SRQ+L+GL YLHS  
Sbjct: 137 VVKCHEMFDQ-NGEIQVLLEFMDKGSLEGA----HVWKEQQLADLSRQILSGLAYLHSRH 196

Query: 185 IIHRDIKPSNLLVNKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGN 244
           I+HRDIKPSNLL+N    VKIADFGVS+I+ +T+D CNS VGT AYMSPER + +   G 
Sbjct: 197 IVHRDIKPSNLLINSAKNVKIADFGVSRILAQTMDPCNSSVGTIAYMSPERINTDLNQGK 256

Query: 245 YNGYAGDIWSLGLTLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPENASEEFRSF 304
           Y+GYAGDIWSLG+++LE YLG FPF P  ++ DWA+LMCAIC  +PP  P  AS EFR F
Sbjct: 257 YDGYAGDIWSLGVSILEFYLGRFPF-PVSRQGDWASLMCAICMSQPPEAPATASPEFRHF 316

Query: 305 VECCLQKESSKRWTAAQLLTHPFVCRESSRSDR 319
           + CCLQ+E  KR +A QLL HPF+ R S   +R
Sbjct: 317 ISCCLQREPGKRRSAMQLLQHPFILRASPSQNR 343

[Close]
BLAST of Lsi02G002520 vs. TAIR10
Match: AT3G06230.1 (AT3G06230.1 MAP kinase kinase 8)

HSP 1 Score: 299.7 bits (766), Expect = 2.2e-81
Identity = 161/306 (52.61%), Postives = 210/306 (68.63%), Query Frame = 1 

Query: 1   MAIVRDRRHLNLRLPDLSDCRP----RFPL-PLPPSSAPAPPAAPSAISSSDLDKLQVLG 60
           M +VRD + LNL+L  +         RFP+ P    SA     A +  S ++LD++ VLG
Sbjct: 1   MVMVRDNQFLNLKLSPIQAPTTIPPCRFPIIPATKVSATVSSCASNTFSVANLDRISVLG 60

Query: 61  HGNGGTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEK 120
            GNGGTV+KV+ K TS  YALK V  + D T      RE+EILR  +SPYV +CH IF+ 
Sbjct: 61  SGNGGTVFKVKDKTTSEIYALKKVKENWDSTS----LREIEILRMVNSPYVAKCHDIFQN 120

Query: 121 PSGDVTILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSN 180
           PSG+V+ILM+YMDLGSL+SL      ++E  LA +SRQVL G +YLH HKI+HRDIKP+N
Sbjct: 121 PSGEVSILMDYMDLGSLESL----RGVTEKQLALMSRQVLEGKNYLHEHKIVHRDIKPAN 180

Query: 181 LLVNKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYG----GNYNGYAG 240
           LL +   EVKIADFGVSKI+ R+L+ CNS+VGT AYMSPER D E  G       N YAG
Sbjct: 181 LLRSSKEEVKIADFGVSKIVVRSLNKCNSFVGTFAYMSPERLDSEADGVTEEDKSNVYAG 240

Query: 241 DIWSLGLTLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPENASEEFRSFVECCLQ 298
           DIWS GLT+LE+ +G++P LP     D A ++CA+CFGEPP  PE  S++ +SF++CCL+
Sbjct: 241 DIWSFGLTMLEILVGYYPMLP-----DQAAIVCAVCFGEPPKAPEECSDDLKSFMDCCLR 293

[Close]
BLAST of Lsi02G002520 vs. NCBI nr
Match: gi|449439485|ref|XP_004137516.1| (PREDICTED: mitogen-activated protein kinase kinase 9 [Cucumis sativus])

HSP 1 Score: 643.7 bits (1659), Expect = 1.7e-181
Identity = 310/316 (98.10%), Postives = 312/316 (98.73%), Query Frame = 1 

Query: 1   MAIVRDRRHLNLRLPDLSDCRPRFPLPLPPSSAPAPPAAPSAISSSDLDKLQVLGHGNGG 60
           MA+VRDRRHLNLRLPDLSDCRPRFPLPLPPSSAP  PAAPSAISSSDLDKLQVLGHGNGG
Sbjct: 1   MALVRDRRHLNLRLPDLSDCRPRFPLPLPPSSAPPAPAAPSAISSSDLDKLQVLGHGNGG 60

Query: 61  TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV 120
           TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV
Sbjct: 61  TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV 120

Query: 121 TILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180
           TILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK
Sbjct: 121 TILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180

Query: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL 240
           NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL
Sbjct: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL 240

Query: 241 LELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPENASEEFRSFVECCLQKESSKRWTA 300
           LELYLGHFPFLP GQRPDWATLMCAICFGEPP LPE+ASEEFRSFVECCLQKESSKRWTA
Sbjct: 241 LELYLGHFPFLPAGQRPDWATLMCAICFGEPPKLPEDASEEFRSFVECCLQKESSKRWTA 300

Query: 301 AQLLTHPFVCRESSRS 317
           AQLLTHPFVCRESSRS
Sbjct: 301 AQLLTHPFVCRESSRS 316

[Close]
BLAST of Lsi02G002520 vs. NCBI nr
Match: gi|659066908|ref|XP_008466859.1| (PREDICTED: mitogen-activated protein kinase kinase 9 [Cucumis melo])

HSP 1 Score: 640.6 bits (1651), Expect = 1.5e-180
Identity = 308/316 (97.47%), Postives = 311/316 (98.42%), Query Frame = 1 

Query: 1   MAIVRDRRHLNLRLPDLSDCRPRFPLPLPPSSAPAPPAAPSAISSSDLDKLQVLGHGNGG 60
           MA+VRDRRHLNLRLPDLSDCRPRFPLPLPPSSAP  PAAPSAISSSDL+KLQVLGHGNGG
Sbjct: 1   MALVRDRRHLNLRLPDLSDCRPRFPLPLPPSSAPPAPAAPSAISSSDLEKLQVLGHGNGG 60

Query: 61  TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV 120
           TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV
Sbjct: 61  TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV 120

Query: 121 TILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180
           TILMEYMDLGSLDSLLKKNSTLSE TLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK
Sbjct: 121 TILMEYMDLGSLDSLLKKNSTLSETTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180

Query: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL 240
           NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL
Sbjct: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL 240

Query: 241 LELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPENASEEFRSFVECCLQKESSKRWTA 300
           LELYLGHFPFLP GQRPDWATLMCAICFGEPP LPE+ASEEFRSFVECCLQKESSKRWTA
Sbjct: 241 LELYLGHFPFLPAGQRPDWATLMCAICFGEPPKLPEDASEEFRSFVECCLQKESSKRWTA 300

Query: 301 AQLLTHPFVCRESSRS 317
           AQLLTHPFVCRESSRS
Sbjct: 301 AQLLTHPFVCRESSRS 316

[Close]
BLAST of Lsi02G002520 vs. NCBI nr
Match: gi|974569602|emb|CUX76643.1| (MAP kinase Kinase 4 [Cucumis sativus])

HSP 1 Score: 638.6 bits (1646), Expect = 5.6e-180
Identity = 308/316 (97.47%), Postives = 310/316 (98.10%), Query Frame = 1 

Query: 1   MAIVRDRRHLNLRLPDLSDCRPRFPLPLPPSSAPAPPAAPSAISSSDLDKLQVLGHGNGG 60
           MA+VRDRRHLNLRLPDLSDCRPRF LPLPPSSAP  PAAPSAISSSDLDKLQVLGHGNGG
Sbjct: 1   MALVRDRRHLNLRLPDLSDCRPRFSLPLPPSSAPPAPAAPSAISSSDLDKLQVLGHGNGG 60

Query: 61  TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV 120
           TVYKVRHKRTS TYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV
Sbjct: 61  TVYKVRHKRTSATYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV 120

Query: 121 TILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180
           TILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK
Sbjct: 121 TILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180

Query: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL 240
           NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL
Sbjct: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL 240

Query: 241 LELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPENASEEFRSFVECCLQKESSKRWTA 300
           LELYLGHFPFLP GQRPDWATLMCAICFGEPP LPE+ASEEFRSFVECCLQKESSKRWTA
Sbjct: 241 LELYLGHFPFLPAGQRPDWATLMCAICFGEPPKLPEDASEEFRSFVECCLQKESSKRWTA 300

Query: 301 AQLLTHPFVCRESSRS 317
           AQLLTHPFVCRESSRS
Sbjct: 301 AQLLTHPFVCRESSRS 316

[Close]
BLAST of Lsi02G002520 vs. NCBI nr
Match: gi|645269696|ref|XP_008240116.1| (PREDICTED: mitogen-activated protein kinase kinase 9-like [Prunus mume])

HSP 1 Score: 543.9 bits (1400), Expect = 1.9e-151
Identity = 257/322 (79.81%), Postives = 291/322 (90.37%), Query Frame = 1 

Query: 1   MAIVRDRRHLNLRLP--DLSDCRPRFPLPLPPSSAPAPP----AAPSAISSSDLDKLQVL 60
           MA++R+RR LNLRLP  + S+CRPRF LPLPP++A        ++ +AIS++DLD LQVL
Sbjct: 1   MALIRERRQLNLRLPLPEPSECRPRFALPLPPTAAATTALTNNSSSAAISAADLDTLQVL 60

Query: 61  GHGNGGTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFE 120
           GHGNGGTVYKVRHKRTSTTYALK+V+GD DPTVRRQ+FREMEILRRTDSPYVV+CH IFE
Sbjct: 61  GHGNGGTVYKVRHKRTSTTYALKLVYGDSDPTVRRQIFREMEILRRTDSPYVVRCHAIFE 120

Query: 121 KPSGDVTILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPS 180
           KPSGD+ I+MEYMD G+L++LLK + T SE  LAHV+RQVLNGL+YLH+HKIIHRDIKP+
Sbjct: 121 KPSGDIGIVMEYMDSGTLETLLKAHGTFSEPKLAHVARQVLNGLNYLHTHKIIHRDIKPT 180

Query: 181 NLLVNKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIW 240
           NLLVN NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDP+TYGGNYNGYA DIW
Sbjct: 181 NLLVNSNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDTYGGNYNGYASDIW 240

Query: 241 SLGLTLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPENASEEFRSFVECCLQKES 300
           SLGLTL+ELY+GHFPFLPPGQRPDWATLMCAICFGEPP+LP+  SEEFRSF+ECCLQKES
Sbjct: 241 SLGLTLMELYMGHFPFLPPGQRPDWATLMCAICFGEPPSLPDGVSEEFRSFMECCLQKES 300

Query: 301 SKRWTAAQLLTHPFVCRESSRS 317
            KRWTAAQLLTHPFVC++ S S
Sbjct: 301 GKRWTAAQLLTHPFVCKDPSIS 322

[Close]
BLAST of Lsi02G002520 vs. NCBI nr
Match: gi|694360170|ref|XP_009360049.1| (PREDICTED: mitogen-activated protein kinase kinase 9-like [Pyrus x bretschneideri])

HSP 1 Score: 536.2 bits (1380), Expect = 3.9e-149
Identity = 252/314 (80.25%), Postives = 283/314 (90.13%), Query Frame = 1 

Query: 1   MAIVRDRRHLNLRLP--DLSDCRPRFPLPLPPSSAPAPPAAPSAISSSDLDKLQVLGHGN 60
           MA++R+RR LNLRLP  DLS+ RPRF LPLPP++     +  SAIS++DL++L+VLGHGN
Sbjct: 1   MALIRERRQLNLRLPLPDLSERRPRFSLPLPPTAVTTNTSC-SAISAADLERLEVLGHGN 60

Query: 61  GGTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSG 120
           GGTVYKVRHKRTS  YA K+V GD DPTVRRQ+FREMEILRRTDSPYVV CH IFE+PSG
Sbjct: 61  GGTVYKVRHKRTSAIYAYKLVQGDSDPTVRRQLFREMEILRRTDSPYVVHCHAIFEQPSG 120

Query: 121 DVTILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLV 180
           D+ ILMEYMD G+L++LLK N T SE  LAHV+RQVLNGL+YLH+HKIIHRDIKP+NLLV
Sbjct: 121 DIGILMEYMDYGTLETLLKSNGTFSEPKLAHVARQVLNGLNYLHTHKIIHRDIKPTNLLV 180

Query: 181 NKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGL 240
           N+NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDP+TYGGNYNGYA DIWSLGL
Sbjct: 181 NRNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDTYGGNYNGYASDIWSLGL 240

Query: 241 TLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPENASEEFRSFVECCLQKESSKRW 300
           TL+ELY+GHFPFLPPGQRPDWATLMCAICFGEPPTLPE  SEEFRSF+ECCLQKES KRW
Sbjct: 241 TLMELYMGHFPFLPPGQRPDWATLMCAICFGEPPTLPEGVSEEFRSFMECCLQKESGKRW 300

Query: 301 TAAQLLTHPFVCRE 313
           +A QLLTHPFVC++
Sbjct: 301 SAGQLLTHPFVCKD 313

The following BLAST results are available for this feature:
BLAST of Lsi02G002520 vs. Swiss-Prot
Match NameE-valueIdentityDescription
M2K9_ARATH1.6e-11366.77Mitogen-activated protein kinase kinase 9 OS=Arabidopsis thaliana GN=MKK9 PE=1 S... [more]
M2K7_ARATH3.1e-10663.61Mitogen-activated protein kinase kinase 7 OS=Arabidopsis thaliana GN=MKK7 PE=1 S... [more]
M2K5_ARATH4.3e-8752.68Mitogen-activated protein kinase kinase 5 OS=Arabidopsis thaliana GN=MKK5 PE=1 S... [more]
M2K8_ARATH7.3e-8752.96Mitogen-activated protein kinase kinase 8 OS=Arabidopsis thaliana GN=MKK8 PE=3 S... [more]
M2K4_ARATH2.3e-8551.35Mitogen-activated protein kinase kinase 4 OS=Arabidopsis thaliana GN=MKK4 PE=1 S... [more]
BLAST of Lsi02G002520 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LQY3_CUCSA1.2e-18198.10Uncharacterized protein OS=Cucumis sativus GN=Csa_1G042980 PE=3 SV=1[more]
A0A0U5PS50_CUCSA3.9e-18097.47MAP kinase Kinase 4 OS=Cucumis sativus GN=MKK4 PE=3 SV=1[more]
W9QZB3_9ROSA9.7e-14776.38Mitogen-activated protein kinase kinase 4 OS=Morus notabilis GN=L484_019336 PE=3... [more]
B9I4T2_POPTR6.3e-14678.46Mitogen-activated protein kinase kinase OS=Populus trichocarpa GN=POPTR_0012s040... [more]
A9PJT7_9ROSI4.1e-14578.53Putative uncharacterized protein OS=Populus trichocarpa x Populus deltoides PE=2... [more]
BLAST of Lsi02G002520 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G73500.18.8e-11566.77 MAP kinase kinase 9[more]
AT1G18350.11.8e-10763.61 MAP kinase kinase 7[more]
AT3G21220.12.4e-8852.68 MAP kinase kinase 5[more]
AT1G51660.11.3e-8651.35 mitogen-activated protein kinase kinase 4[more]
AT3G06230.12.2e-8152.61 MAP kinase kinase 8[more]
BLAST of Lsi02G002520 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449439485|ref|XP_004137516.1|1.7e-18198.10PREDICTED: mitogen-activated protein kinase kinase 9 [Cucumis sativus][more]
gi|659066908|ref|XP_008466859.1|1.5e-18097.47PREDICTED: mitogen-activated protein kinase kinase 9 [Cucumis melo][more]
gi|974569602|emb|CUX76643.1|5.6e-18097.47MAP kinase Kinase 4 [Cucumis sativus][more]
gi|645269696|ref|XP_008240116.1|1.9e-15179.81PREDICTED: mitogen-activated protein kinase kinase 9-like [Prunus mume][more]
gi|694360170|ref|XP_009360049.1|3.9e-14980.25PREDICTED: mitogen-activated protein kinase kinase 9-like [Pyrus x bretschneider... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0006468protein phosphorylation
Vocabulary: Molecular Function
TermDefinition
GO:0005524ATP binding
GO:0004672protein kinase activity
Vocabulary: INTERPRO
TermDefinition
IPR017441Protein_kinase_ATP_BS
IPR011009Kinase-like_dom_sf
IPR008271Ser/Thr_kinase_AS
IPR000719Prot_kinase_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009069 serine family amino acid metabolic process
biological_process GO:0023014 signal transduction by protein phosphorylation
biological_process GO:0007178 transmembrane receptor protein serine/threonine kinase signaling pathway
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005575 cellular_component
molecular_function GO:0005524 ATP binding
molecular_function GO:0004702 receptor signaling protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G002520.1Lsi02G002520.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 50..309
score: 1.4
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 48..309
score: 4.9
IPR000719Protein kinase domainPROFILEPS50011PROTEIN_KINASE_DOMcoord: 48..309
score: 46
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 166..178
scor
IPR011009Protein kinase-like domainunknownSSF56112Protein kinase-like (PK-like)coord: 47..310
score: 9.04
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 54..77
scor
NoneNo IPR availableGENE3DG3DSA:1.10.510.10coord: 112..314
score: 5.7
NoneNo IPR availableGENE3DG3DSA:3.30.200.20coord: 43..111
score: 2.8
NoneNo IPR availablePANTHERPTHR24361MITOGEN-ACTIVATED KINASE KINASE KINASEcoord: 43..315
score: 1.9E
NoneNo IPR availablePANTHERPTHR24361:SF344MITOGEN-ACTIVATED PROTEIN KINASE KINASE 7-RELATEDcoord: 43..315
score: 1.9E