Lsi01G019770 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi01G019770
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Proteinase inhibitor 1
Location	chr01 : 26088610 .. 26089359 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CCTTAAGATACAAAGAGAAGCTCTCAGTAATTAGAAAAAGACATGGCTGATATTCCTTTCCCTATTCCTGGTAAGCCTAATTCAAAACCTTTACATCACTTCTCTAAACTAACAACATTTGAAAATTTTGAATCTCATTTTTAAATATATTTATAAATTGAATTGTTTTTTTATAGAAGTTATTACGTTAAAGATCAATATTTGGAGATGAATTGTAGAACTTGTTATAGTGTTAATGGTTGATTGAACATAAAAGATCATACTATTTATTCACTTTTGTCAAACAAATGGCAAATGATGTTTTCGTTTTTAGAAGTGTTTTCATTTAGTTTAATATTTGGAGATGAATCGTAGAACTTGTTATTTATATACTACTAATTTGTTGTAAAGACTTGTTTGGTAGCCAATCTAAAAATATAAATTCAGATTGATTATCAAACACATATTTGTTAAACTAAGTGAATCTGAAAACACGAAACAATATTCAAATTGCCAACTAAATAAATCCTAAATTATTTGTACTAATTAATGAAATATTATTTTGATCCATTAGGAAAATCATCATGGCCAGAACTTGTGGGAATTGAAGCTGAAATTGCAAAGCTTGTCATACAGAAAGACAATCCTAGAGTGGAAATAATTGACATTATATTAGCCGGTAGTCCAGTGCCTCGTGACTTTAGACAGGATCGAGTTCGAATTTTTGTGAACATACGAAATGTTGCAGTTGAGATACCAATCATTGGTTAA

mRNA sequence

CCTTAAGATACAAAGAGAAGCTCTCAGTAATTAGAAAAAGACATGGCTGATATTCCTTTCCCTATTCCTGAACTTGTGGGAATTGAAGCTGAAATTGCAAAGCTTGTCATACAGAAAGACAATCCTAGAGTGGAAATAATTGACATTATATTAGCCGGTAGTCCAGTGCCTCGTGACTTTAGACAGGATCGAGTTCGAATTTTTGTGAACATACGAAATGTTGCAGTTGAGATACCAATCATTGGTTAA

Coding sequence (CDS)

ATGGCTGATATTCCTTTCCCTATTCCTGAACTTGTGGGAATTGAAGCTGAAATTGCAAAGCTTGTCATACAGAAAGACAATCCTAGAGTGGAAATAATTGACATTATATTAGCCGGTAGTCCAGTGCCTCGTGACTTTAGACAGGATCGAGTTCGAATTTTTGTGAACATACGAAATGTTGCAGTTGAGATACCAATCATTGGTTAA

Protein sequence

MADIPFPIPELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPIIG

Homology

[Close]

BLAST of Lsi01G019770 vs. Swiss-Prot
Match: ICI1_SOLPE (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 1.5e-10
Identity = 32/57 (56.14%), Postives = 42/57 (73.68%), Query Frame = 1


Query: 9   PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIP 66
           PEL+G+ A  AK +I+K+NP +  I I+L GSPV +DFR DRVR+FVNI    V+IP
Sbjct: 52  PELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDVVQIP 108

[Close]

BLAST of Lsi01G019770 vs. Swiss-Prot
Match: ITR1_NICSY (Trypsin inhibitor 1 OS=Nicotiana sylvestris PE=2 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 3.4e-10
Identity = 28/60 (46.67%), Postives = 45/60 (75.00%), Query Frame = 1


Query: 9  PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPIIG 68
          PEL+G+ A+ A+ +IQK+N ++  +  +L GSPV +DFR +RVR+FVN+ +  V+IP +G
Sbjct: 35 PELIGVPAKFAREIIQKENSKLTNVPSVLNGSPVTKDFRCERVRLFVNVLDFVVQIPRVG 94

[Close]

BLAST of Lsi01G019770 vs. Swiss-Prot
Match: ICI1_SOLTU (Proteinase inhibitor 1 OS=Solanum tuberosum PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 1.7e-09
Identity = 28/60 (46.67%), Postives = 42/60 (70.00%), Query Frame = 1


Query: 9   PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPIIG 68
           PEL+G+  ++AK +I+K+N  +  + I+L GSPV  D R DRVR+F NI    V+IP++G
Sbjct: 48  PELIGVPTKLAKGIIEKENSLISNVHILLNGSPVTLDIRCDRVRLFDNILGYVVDIPVVG 107

[Close]

BLAST of Lsi01G019770 vs. Swiss-Prot
Match: ICI1_SOLLC (Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum GN=PIIF PE=2 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 2.9e-09
Identity = 26/59 (44.07%), Postives = 43/59 (72.88%), Query Frame = 1


Query: 9   PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPII 68
           PEL+G+  ++AK +I+K+NP +  I I+L+GSP+  D+  DRVR+F NI    V++P++
Sbjct: 52  PELIGVPTKLAKEIIEKENPSITNIPILLSGSPITLDYLCDRVRLFDNILGFVVQMPVV 110

[Close]

BLAST of Lsi01G019770 vs. Swiss-Prot
Match: IPIB_TOBAC (Proteinase inhibitor I-B OS=Nicotiana tabacum GN=TIMPA PE=2 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 1.9e-08
Identity = 26/60 (43.33%), Postives = 41/60 (68.33%), Query Frame = 1


Query: 9   PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPIIG 68
           PEL+G  A+ A  +IQK+NP++  +  +L G+PV  D R +RVR+FVN+ +  V+ P +G
Sbjct: 48  PELLGTPAKFAMQIIQKENPKLTNVQTVLNGTPVTEDLRCNRVRLFVNVLDFVVQTPQVG 107

[Close]

BLAST of Lsi01G019770 vs. TrEMBL
Match: K4CVX1_SOLLC (Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 1.4e-10
Identity = 36/60 (60.00%), Postives = 45/60 (75.00%), Query Frame = 1


Query: 9   PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPIIG 68
           PEL+G+ A+ AK +IQK+NP V  + I+L GSPV  DFR  RVRI VNI NVAV IP++G
Sbjct: 52  PELIGVPAQYAKGIIQKENPFVTDVQIVLNGSPVTADFRCFRVRIVVNILNVAVSIPVVG 111

[Close]

BLAST of Lsi01G019770 vs. TrEMBL
Match: Q40444_9SOLA (Tumor-related protein (Fragment) OS=Nicotiana glauca x Nicotiana langsdorffii PE=2 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 1.5e-09
Identity = 30/60 (50.00%), Postives = 47/60 (78.33%), Query Frame = 1


Query: 9  PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPIIG 68
          PEL+G+ A+ A+ +IQK+NP++  +  +L GSPV +DFR +RVR+FVN+ +V V+IP +G
Sbjct: 5  PELIGVPAKFAREIIQKENPKLTNVPSVLNGSPVTKDFRCNRVRLFVNVLDVVVQIPRVG 64

[Close]

BLAST of Lsi01G019770 vs. TrEMBL
Match: A0A0A0KME7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G286040 PE=4 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 2.0e-09
Identity = 37/72 (51.39%), Postives = 49/72 (68.06%), Query Frame = 1


Query: 1  MADIPFP----IPELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVN 60
          MADI  P     PELVG++A  AK +I+KDNP VE + ++LAGS    D R DRV +FVN
Sbjct: 1  MADICPPGKNQWPELVGVKATTAKYIIKKDNPNVENVVVLLAGSGTTEDIRCDRVWVFVN 60

Query: 61 IRNVAVEIPIIG 69
          I ++ VE+P +G
Sbjct: 61 IHDLVVEVPKVG 72

[Close]

BLAST of Lsi01G019770 vs. TrEMBL
Match: A0A0A0KMM0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G285030 PE=4 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 2.6e-09
Identity = 37/72 (51.39%), Postives = 49/72 (68.06%), Query Frame = 1


Query: 1  MADIPFPI----PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVN 60
          MADI  P     PELVGI+A IA+ +I+KDNP VE + ++LAG+   +DFR DRV +F N
Sbjct: 1  MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTN 60

Query: 61 IRNVAVEIPIIG 69
          I    VE+P +G
Sbjct: 61 IDGRVVEMPTVG 72

[Close]

BLAST of Lsi01G019770 vs. TrEMBL
Match: M1BHH1_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400017587 PE=4 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 3.4e-09
Identity = 31/60 (51.67%), Postives = 45/60 (75.00%), Query Frame = 1


Query: 9  PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPIIG 68
          PEL+G+ A +A+  IQK+NPR+  +  +L GSPV +DFR DRVR+FVN+ +  V+IP +G
Sbjct: 35 PELLGVPARLARETIQKENPRLTNVGNVLNGSPVTQDFRCDRVRLFVNVLDFVVQIPRVG 94

[Close]

BLAST of Lsi01G019770 vs. TAIR10
Match: AT2G38870.1 (AT2G38870.1 Serine protease inhibitor, potato inhibitor I-type family protein)

HSP 1 Score: 54.3 bits (129), Expect = 3.4e-08
Identity = 28/60 (46.67%), Postives = 38/60 (63.33%), Query Frame = 1


Query: 9  PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPIIG 68
          PEL G   + A +VI+++NP V    +IL GSPV  DFR DRVR+FV+   + V+ P  G
Sbjct: 12 PELTGTNGDYAAVVIERENPTVNAA-VILDGSPVTADFRCDRVRVFVDGNRIVVKTPKSG 70

[Close]

BLAST of Lsi01G019770 vs. TAIR10
Match: AT5G43580.1 (AT5G43580.1 Serine protease inhibitor, potato inhibitor I-type family protein)

HSP 1 Score: 52.4 bits (124), Expect = 1.3e-07
Identity = 28/60 (46.67%), Postives = 42/60 (70.00%), Query Frame = 1


Query: 9  PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPIIG 68
          PEL+G + E AK VI+++NP+++ + IIL G+ VP  F   RV ++VN   + V+IPIIG
Sbjct: 41 PELLGAKGEDAKEVIERENPKMKAV-IILDGTVVPEIFICSRVYVWVNDCGIVVQIPIIG 99

[Close]

BLAST of Lsi01G019770 vs. NCBI nr
Match: gi|460404375|ref|XP_004247658.1| (PREDICTED: wound-induced proteinase inhibitor 1 [Solanum lycopersicum])

HSP 1 Score: 73.2 bits (178), Expect = 2.0e-10
Identity = 36/60 (60.00%), Postives = 45/60 (75.00%), Query Frame = 1


Query: 9   PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPIIG 68
           PEL+G+ A+ AK +IQK+NP V  + I+L GSPV  DFR  RVRI VNI NVAV IP++G
Sbjct: 52  PELIGVPAQYAKGIIQKENPFVTDVQIVLNGSPVTADFRCFRVRIVVNILNVAVSIPVVG 111

[Close]

BLAST of Lsi01G019770 vs. NCBI nr
Match: gi|688425|dbj|BAA05472.1| (tumor-related protein [Nicotiana glauca x Nicotiana langsdorffii])

HSP 1 Score: 69.7 bits (169), Expect = 2.2e-09
Identity = 30/60 (50.00%), Postives = 47/60 (78.33%), Query Frame = 1


Query: 9  PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPIIG 68
          PEL+G+ A+ A+ +IQK+NP++  +  +L GSPV +DFR +RVR+FVN+ +V V+IP +G
Sbjct: 5  PELIGVPAKFAREIIQKENPKLTNVPSVLNGSPVTKDFRCNRVRLFVNVLDVVVQIPRVG 64

[Close]

BLAST of Lsi01G019770 vs. NCBI nr
Match: gi|700195663|gb|KGN50840.1| (hypothetical protein Csa_5G286040 [Cucumis sativus])

HSP 1 Score: 69.3 bits (168), Expect = 2.9e-09
Identity = 37/72 (51.39%), Postives = 49/72 (68.06%), Query Frame = 1


Query: 1  MADIPFP----IPELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVN 60
          MADI  P     PELVG++A  AK +I+KDNP VE + ++LAGS    D R DRV +FVN
Sbjct: 1  MADICPPGKNQWPELVGVKATTAKYIIKKDNPNVENVVVLLAGSGTTEDIRCDRVWVFVN 60

Query: 61 IRNVAVEIPIIG 69
          I ++ VE+P +G
Sbjct: 61 IHDLVVEVPKVG 72

[Close]

BLAST of Lsi01G019770 vs. NCBI nr
Match: gi|970057099|ref|XP_015055202.1| (PREDICTED: trypsin inhibitor 1-like [Solanum pennellii])

HSP 1 Score: 68.9 bits (167), Expect = 3.8e-09
Identity = 30/60 (50.00%), Postives = 46/60 (76.67%), Query Frame = 1


Query: 9  PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPIIG 68
          PEL+G+ A +A+ +IQK+NPR+  I  +  GSPV +DFR DRVR+F+NI ++ V++P +G
Sbjct: 35 PELLGVPARLAREIIQKENPRLTNIGNVQNGSPVTQDFRCDRVRLFINILDIVVQVPRVG 94

[Close]

BLAST of Lsi01G019770 vs. NCBI nr
Match: gi|700195661|gb|KGN50838.1| (hypothetical protein Csa_5G285030 [Cucumis sativus])

HSP 1 Score: 68.9 bits (167), Expect = 3.8e-09
Identity = 37/72 (51.39%), Postives = 49/72 (68.06%), Query Frame = 1


Query: 1  MADIPFPI----PELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVN 60
          MADI  P     PELVGI+A IA+ +I+KDNP VE + ++LAG+   +DFR DRV +F N
Sbjct: 1  MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTN 60

Query: 61 IRNVAVEIPIIG 69
          I    VE+P +G
Sbjct: 61 IDGRVVEMPTVG 72

The following BLAST results are available for this feature:

BLAST of Lsi01G019770 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ICI1_SOLPE	1.5e-10	56.14	Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum PE=3 SV=1	[more]
ITR1_NICSY	3.4e-10	46.67	Trypsin inhibitor 1 OS=Nicotiana sylvestris PE=2 SV=1	[more]
ICI1_SOLTU	1.7e-09	46.67	Proteinase inhibitor 1 OS=Solanum tuberosum PE=3 SV=1	[more]
ICI1_SOLLC	2.9e-09	44.07	Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum GN=PIIF PE=2 SV=1	[more]
IPIB_TOBAC	1.9e-08	43.33	Proteinase inhibitor I-B OS=Nicotiana tabacum GN=TIMPA PE=2 SV=1	[more]

BLAST of Lsi01G019770 vs. TrEMBL


Match Name	E-value	Identity	Description
K4CVX1_SOLLC	1.4e-10	60.00	Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1	[more]
Q40444_9SOLA	1.5e-09	50.00	Tumor-related protein (Fragment) OS=Nicotiana glauca x Nicotiana langsdorffii PE...	[more]
A0A0A0KME7_CUCSA	2.0e-09	51.39	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G286040 PE=4 SV=1	[more]
A0A0A0KMM0_CUCSA	2.6e-09	51.39	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G285030 PE=4 SV=1	[more]
M1BHH1_SOLTU	3.4e-09	51.67	Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400017587 PE=4 SV=1	[more]

BLAST of Lsi01G019770 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G38870.1	3.4e-08	46.67	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43580.1	1.3e-07	46.67	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

BLAST of Lsi01G019770 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|460404375\|ref\|XP_004247658.1\|	2.0e-10	60.00	PREDICTED: wound-induced proteinase inhibitor 1 [Solanum lycopersicum]	[more]
gi\|688425\|dbj\|BAA05472.1\|	2.2e-09	50.00	tumor-related protein [Nicotiana glauca x Nicotiana langsdorffii]	[more]
gi\|700195663\|gb\|KGN50840.1\|	2.9e-09	51.39	hypothetical protein Csa_5G286040 [Cucumis sativus]	[more]
gi\|970057099\|ref\|XP_015055202.1\|	3.8e-09	50.00	PREDICTED: trypsin inhibitor 1-like [Solanum pennellii]	[more]
gi\|700195661\|gb\|KGN50838.1\|	3.8e-09	51.39	hypothetical protein Csa_5G285030 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0009611	response to wounding

Vocabulary: **Molecular Function**
Term	Definition
GO:0004867	serine-type endopeptidase inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR000864	Prot_inh_pot1

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
biological_process	GO:0009611	response to wounding
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G019770.1	Lsi01G019770.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRODOM	PD002604		coord: 9..65 score: 1.0
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRINTS	PR00292	POTATOINHBTR	coord: 45..55 score: 6.7E-9coord: 7..20 score: 6.7E-9coord: 33..44 score: 6.
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 9..68 score: 7.3
IPR000864	Proteinase inhibitor I13, potato inhibitor I	unknown	SSF54654	CI-2 family of serine protease inhibitors	coord: 9..68 score: 8.77
None	No IPR available	GENE3D	G3DSA:3.30.10.10		coord: 9..68 score: 1.5
None	No IPR available	PANTHER	PTHR33091	FAMILY NOT NAMED	coord: 9..68 score: 2.3

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi01G019770	ClCG04G001650	Watermelon (Charleston Gray)	lsiwcgB135

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi01G019770	Cucurbita maxima (Rimu)	cmalsiB792
Lsi01G019770	Cucurbita maxima (Rimu)	cmalsiB788
Lsi01G019770	Cucurbita moschata (Rifu)	cmolsiB776
Lsi01G019770	Cucurbita moschata (Rifu)	cmolsiB780
Lsi01G019770	Cucurbita pepo (Zucchini)	cpelsiB392
Lsi01G019770	Cucurbita pepo (Zucchini)	cpelsiB400
Lsi01G019770	Wild cucumber (PI 183967)	cpilsiB250
Lsi01G019770	Cucumber (Chinese Long) v2	culsiB250
Lsi01G019770	Melon (DHL92) v3.5.1	lsimeB152
Lsi01G019770	Watermelon (97103) v1	lsiwmB146
Lsi01G019770	Cucumber (Gy14) v2	cgyblsiB243
Lsi01G019770	Melon (DHL92) v3.6.1	lsimedB159
Lsi01G019770	Silver-seed gourd	carlsiB257
Lsi01G019770	Silver-seed gourd	carlsiB259
Lsi01G019770	Silver-seed gourd	carlsiB260
Lsi01G019770	Cucumber (Chinese Long) v3	cuclsiB279
Lsi01G019770	Watermelon (97103) v2	lsiwmbB133
Lsi01G019770	Watermelon (97103) v2	lsiwmbB136
Lsi01G019770	Wax gourd	lsiwgoB173