Lsi01G016060 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi01G016060
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Descriptionserine/threonine protein kinase 1
Locationchr01 : 14394431 .. 14395723 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGGCCGCCTTCGACGACAATCCTCCGCCGGGGGGACTCACCCGCGGCGACTCCTTGCCGGAGGGAATTCTCTTCGGGAAATACGAACTCGGTAAGCTTCTCGGATGTGGAGCTTTCGCGAAGGTCTATCATGGCAGAAACGTTTTTACAGGACAGAGCGTTGCGATTAAAGCCGTTAACAAACTCAAGGTTCTCAAAGGCGGTTTCACGGCGCACGTTAAGAGAGAAATCGCTATCATGCGCCGTCTCCGTCATCCTAACGTTGTTCGTCTCTTTGAAGTTCTAGCGACGAAGACGAAGATTTATTTCGTTATGGAGTTCGCGAAAGGAGGAGAACTGTTTGCGAAAGTTTCGAAAGGAAGATTCAGTGAGAATCTCAGCCGTCGGTACTTTCAGCAGTTGATTACCGCCGTTGGATACTGTCATTCCAGAGGAATTTTTCATCGTGATTTGAAACCAGAGAATCTCCTTCTCGATGATAATTTCGATCTCAGAGTTTCGGATTTTGGACTTAGTGCTCTTACAGATCAGATTCGTCCTGACGGATTGCTTCACACGCTTTGTGGAACTCCGGCCTACGTCGCACCGGAAATTCTCGCTAAGAAAGGCTACGACGGCGCTAAAATCGATGTCTGGTCTTGTGGTATAATCCTCTATGTTCTTACTACAGGATATCTTCCTTTCAACGATTCGAATTTAATGGCGATGTATCGGAAGATTTACAGAGGCGATTTCCATTGCCCTAAGTGGACGTCTCCAGATCTGCGACGGTTTCTCTCGAGGCTTCTGGATACGAATCCTGAGACGAGGATTACTGTTGATGAGATTCATCAAGATCCGTGGTTTGTGAAGGGATTTCAGGAGGAGAAGTTTCAACAACTCGAAGATTCCGCTTCCGAGCTTTACGGTGATGTGAACAATGCGAAAGGTTTGAATGCTTTTGATCTTATTTCTTTCTCACCTGGCTTTGATTTGACTGGATTGATGAATGAGACGGAATTTTCTTCCGGCGTTGAGAGGTTTCTCTCCGCTGAGTCGCCTGAAAAGATCATTGTGACGGTCGAGGAGGTTACGAAGTCGGAGAATGCGGCGGTGGAGAAGAACGGCTGGGGAGCTAAAGTGGAAGGACTCGACGGCGGATTCGTCGCCGTGATTGATATTTTCCGGCTAACGGAGGAATTGGTGGTTGTTGAGATTAAAAACAGAGTGAGGGAAATAGATCAAGATCGTCAAATTTGGAAAGATATATTGAGACCTCAACTTGACTGTTTAATCTACCGGTCGGATTGA

mRNA sequence

ATGCCGGCCGCCTTCGACGACAATCCTCCGCCGGGGGGACTCACCCGCGGCGACTCCTTGCCGGAGGGAATTCTCTTCGGGAAATACGAACTCGGTAAGCTTCTCGGATGTGGAGCTTTCGCGAAGGTCTATCATGGCAGAAACGTTTTTACAGGACAGAGCGTTGCGATTAAAGCCGTTAACAAACTCAAGGTTCTCAAAGGCGGTTTCACGGCGCACGTTAAGAGAGAAATCGCTATCATGCGCCGTCTCCGTCATCCTAACGTTGTTCGTCTCTTTGAAGTTCTAGCGACGAAGACGAAGATTTATTTCGTTATGGAGTTCGCGAAAGGAGGAGAACTGTTTGCGAAAGTTTCGAAAGGAAGATTCAGTGAGAATCTCAGCCGTCGGTACTTTCAGCAGTTGATTACCGCCGTTGGATACTGTCATTCCAGAGGAATTTTTCATCGTGATTTGAAACCAGAGAATCTCCTTCTCGATGATAATTTCGATCTCAGAGTTTCGGATTTTGGACTTAGTGCTCTTACAGATCAGATTCGTCCTGACGGATTGCTTCACACGCTTTGTGGAACTCCGGCCTACGTCGCACCGGAAATTCTCGCTAAGAAAGGCTACGACGGCGCTAAAATCGATGTCTGGTCTTGTGGTATAATCCTCTATGTTCTTACTACAGGATATCTTCCTTTCAACGATTCGAATTTAATGGCGATGTATCGGAAGATTTACAGAGGCGATTTCCATTGCCCTAAGTGGACGTCTCCAGATCTGCGACGGTTTCTCTCGAGGCTTCTGGATACGAATCCTGAGACGAGGATTACTGTTGATGAGATTCATCAAGATCCGTGGTTTGTGAAGGGATTTCAGGAGGAGAAGTTTCAACAACTCGAAGATTCCGCTTCCGAGCTTTACGGTGATGTGAACAATGCGAAAGGTTTGAATGCTTTTGATCTTATTTCTTTCTCACCTGGCTTTGATTTGACTGGATTGATGAATGAGACGGAATTTTCTTCCGGCGTTGAGAGGTTTCTCTCCGCTGAGTCGCCTGAAAAGATCATTGTGACGGTCGAGGAGGTTACGAAGTCGGAGAATGCGGCGGTGGAGAAGAACGGCTGGGGAGCTAAAGTGGAAGGACTCGACGGCGGATTCGTCGCCGTGATTGATATTTTCCGGCTAACGGAGGAATTGGTGGTTGTTGAGATTAAAAACAGAGTGAGGGAAATAGATCAAGATCGTCAAATTTGGAAAGATATATTGAGACCTCAACTTGACTGTTTAATCTACCGGTCGGATTGA

Coding sequence (CDS)

ATGCCGGCCGCCTTCGACGACAATCCTCCGCCGGGGGGACTCACCCGCGGCGACTCCTTGCCGGAGGGAATTCTCTTCGGGAAATACGAACTCGGTAAGCTTCTCGGATGTGGAGCTTTCGCGAAGGTCTATCATGGCAGAAACGTTTTTACAGGACAGAGCGTTGCGATTAAAGCCGTTAACAAACTCAAGGTTCTCAAAGGCGGTTTCACGGCGCACGTTAAGAGAGAAATCGCTATCATGCGCCGTCTCCGTCATCCTAACGTTGTTCGTCTCTTTGAAGTTCTAGCGACGAAGACGAAGATTTATTTCGTTATGGAGTTCGCGAAAGGAGGAGAACTGTTTGCGAAAGTTTCGAAAGGAAGATTCAGTGAGAATCTCAGCCGTCGGTACTTTCAGCAGTTGATTACCGCCGTTGGATACTGTCATTCCAGAGGAATTTTTCATCGTGATTTGAAACCAGAGAATCTCCTTCTCGATGATAATTTCGATCTCAGAGTTTCGGATTTTGGACTTAGTGCTCTTACAGATCAGATTCGTCCTGACGGATTGCTTCACACGCTTTGTGGAACTCCGGCCTACGTCGCACCGGAAATTCTCGCTAAGAAAGGCTACGACGGCGCTAAAATCGATGTCTGGTCTTGTGGTATAATCCTCTATGTTCTTACTACAGGATATCTTCCTTTCAACGATTCGAATTTAATGGCGATGTATCGGAAGATTTACAGAGGCGATTTCCATTGCCCTAAGTGGACGTCTCCAGATCTGCGACGGTTTCTCTCGAGGCTTCTGGATACGAATCCTGAGACGAGGATTACTGTTGATGAGATTCATCAAGATCCGTGGTTTGTGAAGGGATTTCAGGAGGAGAAGTTTCAACAACTCGAAGATTCCGCTTCCGAGCTTTACGGTGATGTGAACAATGCGAAAGGTTTGAATGCTTTTGATCTTATTTCTTTCTCACCTGGCTTTGATTTGACTGGATTGATGAATGAGACGGAATTTTCTTCCGGCGTTGAGAGGTTTCTCTCCGCTGAGTCGCCTGAAAAGATCATTGTGACGGTCGAGGAGGTTACGAAGTCGGAGAATGCGGCGGTGGAGAAGAACGGCTGGGGAGCTAAAGTGGAAGGACTCGACGGCGGATTCGTCGCCGTGATTGATATTTTCCGGCTAACGGAGGAATTGGTGGTTGTTGAGATTAAAAACAGAGTGAGGGAAATAGATCAAGATCGTCAAATTTGGAAAGATATATTGAGACCTCAACTTGACTGTTTAATCTACCGGTCGGATTGA

Protein sequence

MPAAFDDNPPPGGLTRGDSLPEGILFGKYELGKLLGCGAFAKVYHGRNVFTGQSVAIKAVNKLKVLKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFAKVSKGRFSENLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRPDGLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFHCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFVKGFQEEKFQQLEDSASELYGDVNNAKGLNAFDLISFSPGFDLTGLMNETEFSSGVERFLSAESPEKIIVTVEEVTKSENAAVEKNGWGAKVEGLDGGFVAVIDIFRLTEELVVVEIKNRVREIDQDRQIWKDILRPQLDCLIYRSD
BLAST of Lsi01G016060 vs. Swiss-Prot
Match: CIPKE_ARATH (CBL-interacting serine/threonine-protein kinase 14 OS=Arabidopsis thaliana GN=CIPK14 PE=1 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 2.8e-158
Identity = 272/414 (65.70%), Postives = 332/414 (80.19%), Query Frame = 1

Query: 23  GILFGKYELGKLLGCGAFAKVYHGRNVFTGQSVAIKAVNKLKVLKGGFTAHVKREIAIMR 82
           G LFGKYE+GKL+GCGAFAKVYHGR+  TGQSVAIK V+K ++ KGG   +++REIAIM 
Sbjct: 16  GQLFGKYEVGKLVGCGAFAKVYHGRSTATGQSVAIKVVSKQRLQKGGLNGNIQREIAIMH 75

Query: 83  RLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFAKVSKGRFSENLSRRYFQQLITAVGYC 142
           RLRHP++VRLFEVLATK+KI+FVMEFAKGGELFAKVSKGRF E+LSRRYFQQLI+AVGYC
Sbjct: 76  RLRHPSIVRLFEVLATKSKIFFVMEFAKGGELFAKVSKGRFCEDLSRRYFQQLISAVGYC 135

Query: 143 HSRGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRPDGLLHTLCGTPAYVAPEILAK 202
           HSRGIFHRDLKPENLLLD+  DL++SDFGLSALTDQIRPDGLLHTLCGTPAYVAPE+LAK
Sbjct: 136 HSRGIFHRDLKPENLLLDEKLDLKISDFGLSALTDQIRPDGLLHTLCGTPAYVAPEVLAK 195

Query: 203 KGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFHCPKWTSPDLRRFLSR 262
           KGYDGAKID+WSCGIIL+VL  GYLPFND NLM MYRKIY+G+F  PKWTSPDLRR L+R
Sbjct: 196 KGYDGAKIDIWSCGIILFVLNAGYLPFNDHNLMVMYRKIYKGEFRIPKWTSPDLRRLLTR 255

Query: 263 LLDTNPETRITVDEIHQDPWFVKGFQEEKFQ-QLEDSASELYGDVNN----AKGLNAFDL 322
           LLDTNP+TRIT++EI  DPWF +G+ +   +  LEDS  +L  D  +    A+ +NAFD+
Sbjct: 256 LLDTNPQTRITIEEIIHDPWFKQGYDDRMSKFHLEDSDMKLPADETDSEMGARRMNAFDI 315

Query: 323 ISFSPGFDLTGLMNETEFSSGVERFLSAESPEKIIVTVEEVTKSENAAV-EKNGWGAKVE 382
           IS SPGF+L+GL  +      VERF+SA + E+++  +EE+  +EN  V +K  WG K+E
Sbjct: 316 ISGSPGFNLSGLFGDARKYDRVERFVSAWTAERVVERLEEIVSAENLTVAKKETWGMKIE 375

Query: 383 GLDGGFVAVIDIFRLTEELVVVEIKNRVREIDQDRQIWKDILRPQLDCLIYRSD 431
           G  G F  V++I +LT+ELV++E++ R R     R +W D LRP    L++ SD
Sbjct: 376 GQKGNFAMVVEINQLTDELVMIEVRKRQRAAASGRDLWTDTLRPFFVELVHESD 429

BLAST of Lsi01G016060 vs. Swiss-Prot
Match: CIPKB_ARATH (CBL-interacting serine/threonine-protein kinase 11 OS=Arabidopsis thaliana GN=CIPK11 PE=1 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 1.1e-138
Identity = 253/405 (62.47%), Postives = 314/405 (77.53%), Query Frame = 1

Query: 25  LFGKYELGKLLGCGAFAKVYHGRNVFTGQSVAIKAVNKLKVLKGGFTAH-VKREIAIMRR 84
           LFGKYELGKLLGCGAFAKV+H R+  TGQSVA+K +NK K+L     A+ +KREI+IMRR
Sbjct: 17  LFGKYELGKLLGCGAFAKVFHARDRRTGQSVAVKILNKKKLLTNPALANNIKREISIMRR 76

Query: 85  LRHPNVVRLFEVLATKTKIYFVMEFAKGGELFAKVSK-GRFSENLSRRYFQQLITAVGYC 144
           L HPN+V+L EV+ATK+KI+F MEF KGGELF K+SK GR SE+LSRRYFQQLI+AVGYC
Sbjct: 77  LSHPNIVKLHEVMATKSKIFFAMEFVKGGELFNKISKHGRLSEDLSRRYFQQLISAVGYC 136

Query: 145 HSRGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRPDGLLHTLCGTPAYVAPEILAK 204
           H+RG++HRDLKPENLL+D+N +L+VSDFGLSALTDQIRPDGLLHTLCGTPAYVAPEIL+K
Sbjct: 137 HARGVYHRDLKPENLLIDENGNLKVSDFGLSALTDQIRPDGLLHTLCGTPAYVAPEILSK 196

Query: 205 KGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFHCPKWTSPDLRRFLSR 264
           KGY+GAK+DVWSCGI+L+VL  GYLPFND N+M MY+KIY+G++  P+W SPDL+RF+SR
Sbjct: 197 KGYEGAKVDVWSCGIVLFVLVAGYLPFNDPNVMNMYKKIYKGEYRFPRWMSPDLKRFVSR 256

Query: 265 LLDTNPETRITVDEIHQDPWFVK-GFQEEKFQQLEDSASELYGDVNNAKGLNAFDLISFS 324
           LLD NPETRIT+DEI +DPWFV+ GF++ KF   E    ++   +   K LNAFDLIS+S
Sbjct: 257 LLDINPETRITIDEILKDPWFVRGGFKQIKFHDDEIEDQKVESSLEAVKSLNAFDLISYS 316

Query: 325 PGFDLTGLMNETEFSSG-VERFLSAESPEKIIVTVEEVTKSENAAVEK---NGWGAKVEG 384
            G DL+GL      SSG  ERFLS +SPE +   VE   + EN  ++K     +G ++EG
Sbjct: 317 SGLDLSGLFAGCSNSSGESERFLSEKSPEMLAEEVEGFAREENLRMKKKKEEEYGFEMEG 376

Query: 385 LDGGFVAVIDIFRLTEELVVVEIKNRVREIDQDRQIWKDILRPQL 423
            +G F   I I RL + LVVVE + R  + D  +++W   LR QL
Sbjct: 377 QNGKFGIGICISRLNDLLVVVEARRRGGDGDCYKEMWNGKLRVQL 421

BLAST of Lsi01G016060 vs. Swiss-Prot
Match: CIPKM_ARATH (CBL-interacting serine/threonine-protein kinase 22 OS=Arabidopsis thaliana GN=CIPK22 PE=2 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 4.5e-124
Identity = 232/404 (57.43%), Postives = 294/404 (72.77%), Query Frame = 1

Query: 25  LFGKYELGKLLGCGAFAKVYHGRNVFT-GQSVAIKAVNKLKVLKGGFTAHVKREIAIMRR 84
           LFGKY+LGKLLG GAFAKVY   ++   G+SVAIK V K K LK G TAHVKREI++MRR
Sbjct: 24  LFGKYDLGKLLGSGAFAKVYQAEDLQNGGESVAIKVVQK-KRLKDGLTAHVKREISVMRR 83

Query: 85  LRHPNVVRLFEVLATKTKIYFVMEFAKGGELFAKVSKGRFSENLSRRYFQQLITAVGYCH 144
           LRHP++V L EVLATKTKIYFVME AKGGELF++V+  RF+E+LSR+YF+QLI+AV YCH
Sbjct: 84  LRHPHIVLLSEVLATKTKIYFVMELAKGGELFSRVTSNRFTESLSRKYFRQLISAVRYCH 143

Query: 145 SRGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRPDGLLHTLCGTPAYVAPEILAKK 204
           +RG+FHRDLKPENLLLD+N DL+VSDFGLSA+ +QI PDG+LHTLCGTPAYVAPE+L KK
Sbjct: 144 ARGVFHRDLKPENLLLDENRDLKVSDFGLSAMKEQIHPDGMLHTLCGTPAYVAPELLLKK 203

Query: 205 GYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFHCPKWTSPDLRRFLSRL 264
           GYDG+K D+WSCG++L++L  GYLPF D N+M +YRKI++  +  P WTS DLR+ L RL
Sbjct: 204 GYDGSKADIWSCGVVLFLLNAGYLPFRDPNIMGLYRKIHKAQYKLPDWTSSDLRKLLRRL 263

Query: 265 LDTNPETRITVDEIHQDPWFVKGFQEEKFQQLEDSASELYGDVNNAKGLNAFDLISFSPG 324
           L+ NPE RITV+EI +DPWF  G    +   ++   ++ Y    N K LNAFDLIS +  
Sbjct: 264 LEPNPELRITVEEILKDPWFNHGVDPSEIIGIQ---ADDYDLEENGKILNAFDLISSASS 323

Query: 325 FDLTGLMNETEFSSGVERFLSAESPEKIIVTVEEVTKSENAAVEKNGWGA-KVEGLDGGF 384
            +L+GL          ++F+S ES   I+  VEEV K  N  + K    A K+EG  G  
Sbjct: 324 SNLSGLFGNFVTPDHCDQFVSDESTAVIMRKVEEVAKQLNLRIAKKKERAIKLEGPHGVA 383

Query: 385 VAVIDIFRLTEELVVVEIKNRVREIDQDRQIWKDILRPQLDCLI 427
             V+ + RLT ELV+VE+KN+ R++     +W D LR +L  LI
Sbjct: 384 NVVVKVRRLTNELVMVEMKNKQRDVG---LVWADALRQKLRRLI 420

BLAST of Lsi01G016060 vs. Swiss-Prot
Match: CIPKI_ARATH (CBL-interacting serine/threonine-protein kinase 18 OS=Arabidopsis thaliana GN=CIPK18 PE=1 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 2.2e-123
Identity = 230/456 (50.44%), Postives = 297/456 (65.13%), Query Frame = 1

Query: 6   DDNPPPGGLTRGDSLPEGILFGKYELGKLLGCGAFAKVYHGRNVFTGQSVAIKAVNKLKV 65
           D N  P       S    IL GKYELGKLLG G FAKVY  +N+ +G  VAIK ++K K+
Sbjct: 51  DGNISPQSPRSPRSPRNNILMGKYELGKLLGHGTFAKVYLAQNIKSGDKVAIKVIDKEKI 110

Query: 66  LKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFAKVSKGRFSE 125
           +K G  AH+KREI+I+RR+RHP +V LFEV+ATK+KIYFVME+  GGELF  V+KGR  E
Sbjct: 111 MKSGLVAHIKREISILRRVRHPYIVHLFEVMATKSKIYFVMEYVGGGELFNTVAKGRLPE 170

Query: 126 NLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRPDGLL 185
             +RRYFQQLI++V +CH RG++HRDLKPENLLLD+  +L+VSDFGLSA+ +Q+R DGL 
Sbjct: 171 ETARRYFQQLISSVSFCHGRGVYHRDLKPENLLLDNKGNLKVSDFGLSAVAEQLRQDGLC 230

Query: 186 HTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGD 245
           HT CGTPAY+APE+L +KGYD AK DVWSCG+IL+VL  G++PF D N+M MY+KIY+G+
Sbjct: 231 HTFCGTPAYIAPEVLTRKGYDAAKADVWSCGVILFVLMAGHIPFYDKNIMVMYKKIYKGE 290

Query: 246 FHCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFVKGFQEEKF--------QQLED 305
           F CP+W S DL R L+RLLDTNP+TRIT+ EI ++ WF KGF+  KF        ++ ED
Sbjct: 291 FRCPRWFSSDLVRLLTRLLDTNPDTRITIPEIMKNRWFKKGFKHVKFYIEDDKLCREDED 350

Query: 306 SASEL----------------------YGDVNNAKGLNAFDLISFSPGFDLTGLMNETEF 365
              E                        G +     LNAFD+ISFS GFDL+GL  E E 
Sbjct: 351 EEEEASSSGRSSTVSESDAEFDVKRMGIGSMPRPSSLNAFDIISFSSGFDLSGLFEE-EG 410

Query: 366 SSGVERFLSAESPEKIIVTVEEVTKSENAAVEKNGWGAKV----EGLDGGFVAVIDIFRL 425
             G  RF+S     KII  +EE+ K  +  V K  W  ++    EG  G      +IF L
Sbjct: 411 GEGT-RFVSGAPVSKIISKLEEIAKIVSFTVRKKEWSLRLEGCREGAKGPLTIAAEIFEL 470

Query: 426 TEELVVVEIKNRVREIDQDRQIWKDILRPQLDCLIY 428
           T  LVVVE+K +  + ++  +     LRP+L+ LI+
Sbjct: 471 TPSLVVVEVKKKGGDREEYEEFCNKELRPELEKLIH 504

BLAST of Lsi01G016060 vs. Swiss-Prot
Match: CIPKJ_ARATH (CBL-interacting serine/threonine-protein kinase 19 OS=Arabidopsis thaliana GN=CIPK19 PE=2 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 1.2e-121
Identity = 225/443 (50.79%), Postives = 295/443 (66.59%), Query Frame = 1

Query: 22  EGILFGKYELGKLLGCGAFAKVYHGRNVFTGQSVAIKAVNKLKVLKGGFTAHVKREIAIM 81
           + ++ GKYE+G+LLG G FAKVY  RN  +G+SVAIK ++K KVLK G  AH+KREI+I+
Sbjct: 21  QALILGKYEMGRLLGHGTFAKVYLARNAQSGESVAIKVIDKEKVLKSGLIAHIKREISIL 80

Query: 82  RRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFAKVSKGRFSENLSRRYFQQLITAVGY 141
           RR+RHPN+V+LFEV+ATK+KIYFVME+ KGGELF KV+KGR  E ++R+YFQQLI+AV +
Sbjct: 81  RRVRHPNIVQLFEVMATKSKIYFVMEYVKGGELFNKVAKGRLKEEMARKYFQQLISAVSF 140

Query: 142 CHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRPDGLLHTLCGTPAYVAPEILA 201
           CH RG++HRDLKPENLLLD+N +L+VSDFGLSA++DQIR DGL HT CGTPAYVAPE+LA
Sbjct: 141 CHFRGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTPAYVAPEVLA 200

Query: 202 KKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFHCPKWTSPDLRRFLS 261
           +KGYDGAK+D+WSCG+IL+VL  G+LPF+D N+MAMY+KIYRGDF CP+W   ++ R L 
Sbjct: 201 RKGYDGAKVDIWSCGVILFVLMAGFLPFHDRNVMAMYKKIYRGDFRCPRWFPVEINRLLI 260

Query: 262 RLLDTNPETRITVDEIHQDPWFVKGFQEEKF----------------------------- 321
           R+L+T PE R T+ +I +  WF KGF+  KF                             
Sbjct: 261 RMLETKPERRFTMPDIMETSWFKKGFKHIKFYVEDDHQLCNVADDDEIESIESVSGRSST 320

Query: 322 -QQLEDSAS----ELYGDVNNAKGLNAFDLISFSPGFDLTGLMNETEFSSGVERFLSAES 381
             + ED  S       G +     LNAFDLISFSPGFDL+GL  +    S   RF+S   
Sbjct: 321 VSEPEDFESFDGRRRGGSMPRPASLNAFDLISFSPGFDLSGLFEDDGEGS---RFVSGAP 380

Query: 382 PEKIIVTVEEVTKSENAAVEKNGWGAKVEG-----LDGGFVAVIDIFRLTEELVVVEIKN 426
             +II  +EE+ +  +  V K      +EG     + G      +IF LT  LVVVE+K 
Sbjct: 381 VGQIISKLEEIARIVSFTVRKKDCKVSLEGSREGSMKGPLSIAAEIFELTPALVVVEVKK 440

BLAST of Lsi01G016060 vs. TrEMBL
Match: A0A0A0L2V2_CUCSA (Non-specific serine/threonine protein kinase OS=Cucumis sativus GN=Csa_4G331590 PE=3 SV=1)

HSP 1 Score: 802.7 bits (2072), Expect = 2.1e-229
Identity = 400/433 (92.38%), Postives = 413/433 (95.38%), Query Frame = 1

Query: 1   MPAAFDDNPPPGGLTRGDSLPEGILFGKYELGKLLGCGAFAKVYHGRNVFTGQSVAIKAV 60
           MPA  DD PPPG L R DSLPEGILFGKYELGKLLGCGAFAKVYHGRNV TGQSVAIKAV
Sbjct: 1   MPAFHDDTPPPG-LPRADSLPEGILFGKYELGKLLGCGAFAKVYHGRNVLTGQSVAIKAV 60

Query: 61  NKLKVLKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFAKVSK 120
           NKLKVLKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATKTKIYFVME+AKGGELF KVSK
Sbjct: 61  NKLKVLKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATKTKIYFVMEYAKGGELFGKVSK 120

Query: 121 GRFSENLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIR 180
           GRFSENLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSAL DQIR
Sbjct: 121 GRFSENLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSALADQIR 180

Query: 181 PDGLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRK 240
           PDGLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLT GYLPFNDSNLMAMYRK
Sbjct: 181 PDGLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTAGYLPFNDSNLMAMYRK 240

Query: 241 IYRGDFHCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFVKGFQEEK--FQQLEDS 300
           IYRGDF CPKWTSPDL+RF+SRLLDTNPE+RITVDEI +DPWFVKGFQEEK  FQ+L+DS
Sbjct: 241 IYRGDFRCPKWTSPDLQRFISRLLDTNPESRITVDEIQRDPWFVKGFQEEKFQFQELDDS 300

Query: 301 ASELYGDVNNAKGLNAFDLISFSPGFDLTGLM-NETEFSSGVERFLSAESPEKIIVTVEE 360
           AS++YGDVNNAK LNAFDLISFSPGFDLTGL+  ETEFSS VERFLSAESPEKIIVTVEE
Sbjct: 301 ASDVYGDVNNAKCLNAFDLISFSPGFDLTGLIKEETEFSSCVERFLSAESPEKIIVTVEE 360

Query: 361 VTKSENAAVEKNGWGAKVEGLDGGFVAVIDIFRLTEELVVVEIKNRVREIDQDRQIWKDI 420
           VTKSENAAVEKNGWG KVEGLDGGFV VIDIFRLTEELVVVEIKNRVREI+QDRQIWKD+
Sbjct: 361 VTKSENAAVEKNGWGMKVEGLDGGFVVVIDIFRLTEELVVVEIKNRVREIEQDRQIWKDV 420

Query: 421 LRPQLDCLIYRSD 431
           LRPQL+CLIYRSD
Sbjct: 421 LRPQLECLIYRSD 432

BLAST of Lsi01G016060 vs. TrEMBL
Match: M5WAX9_PRUPE (Non-specific serine/threonine protein kinase OS=Prunus persica GN=PRUPE_ppa005902mg PE=3 SV=1)

HSP 1 Score: 614.4 bits (1583), Expect = 1.1e-172
Identity = 299/407 (73.46%), Postives = 351/407 (86.24%), Query Frame = 1

Query: 25  LFGKYELGKLLGCGAFAKVYHGRNVFTGQSVAIKAVNKLKVLKGGFTAHVKREIAIMRRL 84
           LFGKYELG+LLG GAFAKVYH RNV TGQSVAIKAV+K KVLKGGFTAHVKREI+IMRRL
Sbjct: 27  LFGKYELGRLLGRGAFAKVYHARNVDTGQSVAIKAVSKQKVLKGGFTAHVKREISIMRRL 86

Query: 85  RHPNVVRLFEVLATKTKIYFVMEFAKGGELFAKVSKGRFSENLSRRYFQQLITAVGYCHS 144
           RHP++V+L+EVLATKTKIYF+MEFAKGGELFAK+SKGRFSE+LSRRYFQQLI+A+GYCHS
Sbjct: 87  RHPHIVKLYEVLATKTKIYFIMEFAKGGELFAKISKGRFSEDLSRRYFQQLISAIGYCHS 146

Query: 145 RGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRPDGLLHTLCGTPAYVAPEILAKKG 204
           RG+FHRDLKPENLLLD+N++L+VSDFGLSA+T+Q+RPDGLLHTLCGTPAYVAPEIL KKG
Sbjct: 147 RGVFHRDLKPENLLLDENWNLKVSDFGLSAVTEQVRPDGLLHTLCGTPAYVAPEILGKKG 206

Query: 205 YDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFHCPKWTSPDLRRFLSRLL 264
           Y+GAK+D+WSCGIIL+VL +GYLPFND NLM MYRKIY+GDF  P+WTSPDLRR +S LL
Sbjct: 207 YEGAKVDIWSCGIILFVLNSGYLPFNDQNLMVMYRKIYKGDFRFPRWTSPDLRRLISSLL 266

Query: 265 DTNPETRITVDEIHQDPWFVKGFQEEKFQQLEDSASELYGDVNNAKGLNAFDLISFSPGF 324
           DTNPETRITVDEI +DPWF KG++E KF   ED   +   D +N   LNAFDLISFS GF
Sbjct: 267 DTNPETRITVDEILKDPWFGKGYEEIKF-HFEDFELKDLRDGDNDTSLNAFDLISFSSGF 326

Query: 325 DLTGLMNETEFSSGVERFLSAESPEKIIVTVEEVTKSENAAV-EKNGWGAKVEGLDGGFV 384
           D++GL +++E S   ERF+SAE+PE II  VEE+ K+EN AV EK  WGAK+ G +G FV
Sbjct: 327 DISGLFSKSEISDCGERFISAETPETIIEKVEEIAKAENVAVMEKKSWGAKLAGQNGNFV 386

Query: 385 AVIDIFRLTEELVVVEIKNRVREIDQDRQIWKDILRPQLDCLIYRSD 431
            +I I+RLTEELVVVE+  R R+ +  ++IWKD LRPQL CLIYR +
Sbjct: 387 VMIGIYRLTEELVVVELNLRQRQSECSQRIWKDKLRPQLSCLIYRPE 432

BLAST of Lsi01G016060 vs. TrEMBL
Match: A0A0U3BTT2_MALDO (Non-specific serine/threonine protein kinase OS=Malus domestica GN=CIPK11 PE=2 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 1.5e-171
Identity = 298/416 (71.63%), Postives = 353/416 (84.86%), Query Frame = 1

Query: 17  GDSLPEGILFGKYELGKLLGCGAFAKVYHGRNVFTGQSVAIKAVNKLKVLKGGFTAHVKR 76
           G S  E  LFGKYE+G+LLGCGAFAKVYH RNV TGQSVAIKAV+K KV+K GFTAHVKR
Sbjct: 13  GSSAGEVNLFGKYEVGRLLGCGAFAKVYHARNVHTGQSVAIKAVSKQKVVKDGFTAHVKR 72

Query: 77  EIAIMRRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFAKVSKGRFSENLSRRYFQQLI 136
           EI+IMRRLRHP++V+LFEVLATKTKIYF+MEFAKGGELFAK+SKGRFSE+LSRRYFQQLI
Sbjct: 73  EISIMRRLRHPHIVKLFEVLATKTKIYFIMEFAKGGELFAKISKGRFSEDLSRRYFQQLI 132

Query: 137 TAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRPDGLLHTLCGTPAYVA 196
           +AVGYCHSRG+FHRDLKPENLLLD+N++L+VSDFGLSA+T+Q+RPDGLL+TLCGTPAYVA
Sbjct: 133 SAVGYCHSRGVFHRDLKPENLLLDENWNLKVSDFGLSAVTEQVRPDGLLYTLCGTPAYVA 192

Query: 197 PEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFHCPKWTSPDL 256
           PEILAKKGYDGAKID+WSCGI+L+VL  GYLPFND NLM MYRKIY+GD+  PKWTSPDL
Sbjct: 193 PEILAKKGYDGAKIDIWSCGIVLFVLNAGYLPFNDPNLMVMYRKIYKGDYRVPKWTSPDL 252

Query: 257 RRFLSRLLDTNPETRITVDEIHQDPWFVKGFQEEKFQQLEDSASELY-GDVNNAKGLNAF 316
           +R +SRLLDTN ETRITVDEI +DPWF KG+QE KF   +    ++  GD +    LNAF
Sbjct: 253 KRLISRLLDTNSETRITVDEILKDPWFAKGYQEIKFHFEDFDLKDMRDGDSDETSSLNAF 312

Query: 317 DLISFSPGFDLTGLMNETEFSSGVERFLSAESPEKIIVTVEEVTKSENAAV-EKNGWGAK 376
           DLISFS GFD++GL +++  S   ERF+SAE+PE+I+  VEEV K+ + AV EK  WGAK
Sbjct: 313 DLISFSSGFDISGLFSKSGISDCGERFVSAETPERIMEKVEEVAKAGDMAVTEKKSWGAK 372

Query: 377 VEGLDGGFVAVIDIFRLTEELVVVEIKNRVREIDQDRQIWKDILRPQLDCLIYRSD 431
           + G +G FV +I I+RLTE+LVVVE+K R RE +   QIWKD LRP+L CL+YR +
Sbjct: 373 LAGQNGNFVVMIGIYRLTEKLVVVELKLRQREAECGPQIWKDKLRPELSCLMYRPE 428

BLAST of Lsi01G016060 vs. TrEMBL
Match: W9QMI1_9ROSA (Non-specific serine/threonine protein kinase OS=Morus notabilis GN=L484_021953 PE=3 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 2.1e-168
Identity = 299/413 (72.40%), Postives = 352/413 (85.23%), Query Frame = 1

Query: 17  GDSLPEGILFGKYELGKLLGCGAFAKVYHGRNVFTGQSVAIKAVNKLKVLKGGFTAHVKR 76
           GD+ PE  LFGKYELGKLLG GAFAKVYH RNV TGQSVAIKAV+K KV+KGGF A VKR
Sbjct: 18  GDASPEMNLFGKYELGKLLGRGAFAKVYHARNVGTGQSVAIKAVSKQKVVKGGFVAQVKR 77

Query: 77  EIAIMRRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFAKVSKGRFSENLSRRYFQQLI 136
           EIAIMRRL HPNVV+L EVLATKTKIYFVMEFAKGGELFAKV+KGRFSE+LSRRYFQQLI
Sbjct: 78  EIAIMRRLSHPNVVKLMEVLATKTKIYFVMEFAKGGELFAKVAKGRFSEDLSRRYFQQLI 137

Query: 137 TAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRPDGLLHTLCGTPAYVA 196
           +AVGYCHSRG+FHRDLKPENLLLD+N+DL+VSDFGLSA++DQI+PDGLLHTLCGTPAYVA
Sbjct: 138 SAVGYCHSRGVFHRDLKPENLLLDENWDLKVSDFGLSAVSDQIQPDGLLHTLCGTPAYVA 197

Query: 197 PEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFHCPKWTSPDL 256
           PEILAKKGYDGAK+DVW+CGIIL+VL  GYLPFND NLM MYRKIY+G+F  PKWTSP+L
Sbjct: 198 PEILAKKGYDGAKVDVWTCGIILFVLNAGYLPFNDPNLMTMYRKIYKGEFRFPKWTSPEL 257

Query: 257 RRFLSRLLDTNPETRITVDEIHQDPWFVKGFQEEKFQQLEDSASELYGDVNNAKGLNAFD 316
           RRF+SRLLD NPETRIT+D+I +DPWF KGF+E KF+  E++  E +  V N+K LNAFD
Sbjct: 258 RRFISRLLDINPETRITIDQILRDPWFRKGFRERKFRP-EENFQESH-SVVNSKSLNAFD 317

Query: 317 LISFSPGFDLTGLMNETEFSSGVERFLSAESPEKIIVTVEEVTKSENAAVEKN-GWGAKV 376
           +ISFS GF+L+GL +E   S   ERF+SAE+ E I+  VEEV KSE+  V K   W A+V
Sbjct: 318 IISFSSGFNLSGLFSECGVSESEERFVSAETVEGILERVEEVAKSESLTVTKKIDWRARV 377

Query: 377 EGLDGGFVAVIDIFRLTEELVVVEIKNRVREIDQDRQIWKDILRPQLDCLIYR 429
           EG +G FVAV+++ RLT++LV+VE+K + RE+    QIWKD LRP+LD L+++
Sbjct: 378 EGQNGDFVAVVEVHRLTDKLVLVEVKQKERELVTGLQIWKDKLRPKLDGLVFQ 428

BLAST of Lsi01G016060 vs. TrEMBL
Match: A0A067JG26_JATCU (Non-specific serine/threonine protein kinase OS=Jatropha curcas GN=JCGZ_00369 PE=3 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 6.7e-167
Identity = 299/411 (72.75%), Postives = 343/411 (83.45%), Query Frame = 1

Query: 21  PEGILFGKYELGKLLGCGAFAKVYHGRNVFTGQSVAIKAVNKLKVLKGGFTAHVKREIAI 80
           PE  LF KYELGKLLG GAFAKVYH RNV TGQSVAIKAV+K KVLKGGF AHVKREI+I
Sbjct: 10  PEINLFDKYELGKLLGVGAFAKVYHARNVRTGQSVAIKAVSKQKVLKGGFMAHVKREISI 69

Query: 81  MRRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFAKVSKGRFSENLSRRYFQQLITAVG 140
           MRRLRHP+VV+L EVLATK+KI+FVMEFAKGGELFAKV+KGRFSE+LSRRYFQQLITAVG
Sbjct: 70  MRRLRHPHVVKLLEVLATKSKIFFVMEFAKGGELFAKVAKGRFSEDLSRRYFQQLITAVG 129

Query: 141 YCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRPDGLLHTLCGTPAYVAPEIL 200
           YCH RG+FHRDLKPENLLLD+N+DL++SDFGLSAL DQI PDGLLHTLCGTPAYVAPEIL
Sbjct: 130 YCHDRGVFHRDLKPENLLLDENWDLKISDFGLSALRDQIPPDGLLHTLCGTPAYVAPEIL 189

Query: 201 AKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFHCPKWTSPDLRRFL 260
            KKGYDGAK+DVWSCG+ILYVL  GYLPFND+NLM +YRKIYRG F  P+W SPDLRRFL
Sbjct: 190 GKKGYDGAKVDVWSCGVILYVLIAGYLPFNDTNLMVLYRKIYRGQFKFPRWISPDLRRFL 249

Query: 261 SRLLDTNPETRITVDEIHQDPWFVKGFQEEKFQQLEDSASELYGDVNNAKGLNAFDLISF 320
           SRLLD NPETRITVDEI +DPWF+K ++E KF  LED  S++    +N K LNAFD+ISF
Sbjct: 250 SRLLDPNPETRITVDEILRDPWFIKDYKEAKF-HLEDFDSKVQ---DNHKRLNAFDIISF 309

Query: 321 SPGFDLTGLMNETEFSSGVERFLSAESPEKIIVTVEEVTKSENAAV-EKNGWGAKVEGLD 380
           S GFDL+ L N+ + S+  ERF+S++SP KII  VEEV K EN  V +K  WG K+EGL+
Sbjct: 310 SSGFDLSCLFNDNDISACSERFVSSKSPAKIIERVEEVFKGENVRVTKKKKWGVKLEGLN 369

Query: 381 GGFVAVIDIFRLTEELVVVEIKNRVREIDQDRQIWKDILRPQLDCLIYRSD 431
           G FV  IDIF LTE+LVVVE+  +   ++   +IWKDIL PQL  LIY+ +
Sbjct: 370 GCFVMAIDIFPLTEQLVVVEVTGKEANVNPGHEIWKDILWPQLGGLIYQPE 416

BLAST of Lsi01G016060 vs. TAIR10
Match: AT5G01820.1 (AT5G01820.1 serine/threonine protein kinase 1)

HSP 1 Score: 559.7 bits (1441), Expect = 1.6e-159
Identity = 272/414 (65.70%), Postives = 332/414 (80.19%), Query Frame = 1

Query: 23  GILFGKYELGKLLGCGAFAKVYHGRNVFTGQSVAIKAVNKLKVLKGGFTAHVKREIAIMR 82
           G LFGKYE+GKL+GCGAFAKVYHGR+  TGQSVAIK V+K ++ KGG   +++REIAIM 
Sbjct: 16  GQLFGKYEVGKLVGCGAFAKVYHGRSTATGQSVAIKVVSKQRLQKGGLNGNIQREIAIMH 75

Query: 83  RLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFAKVSKGRFSENLSRRYFQQLITAVGYC 142
           RLRHP++VRLFEVLATK+KI+FVMEFAKGGELFAKVSKGRF E+LSRRYFQQLI+AVGYC
Sbjct: 76  RLRHPSIVRLFEVLATKSKIFFVMEFAKGGELFAKVSKGRFCEDLSRRYFQQLISAVGYC 135

Query: 143 HSRGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRPDGLLHTLCGTPAYVAPEILAK 202
           HSRGIFHRDLKPENLLLD+  DL++SDFGLSALTDQIRPDGLLHTLCGTPAYVAPE+LAK
Sbjct: 136 HSRGIFHRDLKPENLLLDEKLDLKISDFGLSALTDQIRPDGLLHTLCGTPAYVAPEVLAK 195

Query: 203 KGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFHCPKWTSPDLRRFLSR 262
           KGYDGAKID+WSCGIIL+VL  GYLPFND NLM MYRKIY+G+F  PKWTSPDLRR L+R
Sbjct: 196 KGYDGAKIDIWSCGIILFVLNAGYLPFNDHNLMVMYRKIYKGEFRIPKWTSPDLRRLLTR 255

Query: 263 LLDTNPETRITVDEIHQDPWFVKGFQEEKFQ-QLEDSASELYGDVNN----AKGLNAFDL 322
           LLDTNP+TRIT++EI  DPWF +G+ +   +  LEDS  +L  D  +    A+ +NAFD+
Sbjct: 256 LLDTNPQTRITIEEIIHDPWFKQGYDDRMSKFHLEDSDMKLPADETDSEMGARRMNAFDI 315

Query: 323 ISFSPGFDLTGLMNETEFSSGVERFLSAESPEKIIVTVEEVTKSENAAV-EKNGWGAKVE 382
           IS SPGF+L+GL  +      VERF+SA + E+++  +EE+  +EN  V +K  WG K+E
Sbjct: 316 ISGSPGFNLSGLFGDARKYDRVERFVSAWTAERVVERLEEIVSAENLTVAKKETWGMKIE 375

Query: 383 GLDGGFVAVIDIFRLTEELVVVEIKNRVREIDQDRQIWKDILRPQLDCLIYRSD 431
           G  G F  V++I +LT+ELV++E++ R R     R +W D LRP    L++ SD
Sbjct: 376 GQKGNFAMVVEINQLTDELVMIEVRKRQRAAASGRDLWTDTLRPFFVELVHESD 429

BLAST of Lsi01G016060 vs. TAIR10
Match: AT2G30360.1 (AT2G30360.1 SOS3-interacting protein 4)

HSP 1 Score: 494.6 bits (1272), Expect = 6.2e-140
Identity = 253/405 (62.47%), Postives = 314/405 (77.53%), Query Frame = 1

Query: 25  LFGKYELGKLLGCGAFAKVYHGRNVFTGQSVAIKAVNKLKVLKGGFTAH-VKREIAIMRR 84
           LFGKYELGKLLGCGAFAKV+H R+  TGQSVA+K +NK K+L     A+ +KREI+IMRR
Sbjct: 17  LFGKYELGKLLGCGAFAKVFHARDRRTGQSVAVKILNKKKLLTNPALANNIKREISIMRR 76

Query: 85  LRHPNVVRLFEVLATKTKIYFVMEFAKGGELFAKVSK-GRFSENLSRRYFQQLITAVGYC 144
           L HPN+V+L EV+ATK+KI+F MEF KGGELF K+SK GR SE+LSRRYFQQLI+AVGYC
Sbjct: 77  LSHPNIVKLHEVMATKSKIFFAMEFVKGGELFNKISKHGRLSEDLSRRYFQQLISAVGYC 136

Query: 145 HSRGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRPDGLLHTLCGTPAYVAPEILAK 204
           H+RG++HRDLKPENLL+D+N +L+VSDFGLSALTDQIRPDGLLHTLCGTPAYVAPEIL+K
Sbjct: 137 HARGVYHRDLKPENLLIDENGNLKVSDFGLSALTDQIRPDGLLHTLCGTPAYVAPEILSK 196

Query: 205 KGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFHCPKWTSPDLRRFLSR 264
           KGY+GAK+DVWSCGI+L+VL  GYLPFND N+M MY+KIY+G++  P+W SPDL+RF+SR
Sbjct: 197 KGYEGAKVDVWSCGIVLFVLVAGYLPFNDPNVMNMYKKIYKGEYRFPRWMSPDLKRFVSR 256

Query: 265 LLDTNPETRITVDEIHQDPWFVK-GFQEEKFQQLEDSASELYGDVNNAKGLNAFDLISFS 324
           LLD NPETRIT+DEI +DPWFV+ GF++ KF   E    ++   +   K LNAFDLIS+S
Sbjct: 257 LLDINPETRITIDEILKDPWFVRGGFKQIKFHDDEIEDQKVESSLEAVKSLNAFDLISYS 316

Query: 325 PGFDLTGLMNETEFSSG-VERFLSAESPEKIIVTVEEVTKSENAAVEK---NGWGAKVEG 384
            G DL+GL      SSG  ERFLS +SPE +   VE   + EN  ++K     +G ++EG
Sbjct: 317 SGLDLSGLFAGCSNSSGESERFLSEKSPEMLAEEVEGFAREENLRMKKKKEEEYGFEMEG 376

Query: 385 LDGGFVAVIDIFRLTEELVVVEIKNRVREIDQDRQIWKDILRPQL 423
            +G F   I I RL + LVVVE + R  + D  +++W   LR QL
Sbjct: 377 QNGKFGIGICISRLNDLLVVVEARRRGGDGDCYKEMWNGKLRVQL 421

BLAST of Lsi01G016060 vs. TAIR10
Match: AT2G38490.1 (AT2G38490.1 CBL-interacting protein kinase 22)

HSP 1 Score: 446.0 bits (1146), Expect = 2.5e-125
Identity = 232/404 (57.43%), Postives = 294/404 (72.77%), Query Frame = 1

Query: 25  LFGKYELGKLLGCGAFAKVYHGRNVFT-GQSVAIKAVNKLKVLKGGFTAHVKREIAIMRR 84
           LFGKY+LGKLLG GAFAKVY   ++   G+SVAIK V K K LK G TAHVKREI++MRR
Sbjct: 48  LFGKYDLGKLLGSGAFAKVYQAEDLQNGGESVAIKVVQK-KRLKDGLTAHVKREISVMRR 107

Query: 85  LRHPNVVRLFEVLATKTKIYFVMEFAKGGELFAKVSKGRFSENLSRRYFQQLITAVGYCH 144
           LRHP++V L EVLATKTKIYFVME AKGGELF++V+  RF+E+LSR+YF+QLI+AV YCH
Sbjct: 108 LRHPHIVLLSEVLATKTKIYFVMELAKGGELFSRVTSNRFTESLSRKYFRQLISAVRYCH 167

Query: 145 SRGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRPDGLLHTLCGTPAYVAPEILAKK 204
           +RG+FHRDLKPENLLLD+N DL+VSDFGLSA+ +QI PDG+LHTLCGTPAYVAPE+L KK
Sbjct: 168 ARGVFHRDLKPENLLLDENRDLKVSDFGLSAMKEQIHPDGMLHTLCGTPAYVAPELLLKK 227

Query: 205 GYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFHCPKWTSPDLRRFLSRL 264
           GYDG+K D+WSCG++L++L  GYLPF D N+M +YRKI++  +  P WTS DLR+ L RL
Sbjct: 228 GYDGSKADIWSCGVVLFLLNAGYLPFRDPNIMGLYRKIHKAQYKLPDWTSSDLRKLLRRL 287

Query: 265 LDTNPETRITVDEIHQDPWFVKGFQEEKFQQLEDSASELYGDVNNAKGLNAFDLISFSPG 324
           L+ NPE RITV+EI +DPWF  G    +   ++   ++ Y    N K LNAFDLIS +  
Sbjct: 288 LEPNPELRITVEEILKDPWFNHGVDPSEIIGIQ---ADDYDLEENGKILNAFDLISSASS 347

Query: 325 FDLTGLMNETEFSSGVERFLSAESPEKIIVTVEEVTKSENAAVEKNGWGA-KVEGLDGGF 384
            +L+GL          ++F+S ES   I+  VEEV K  N  + K    A K+EG  G  
Sbjct: 348 SNLSGLFGNFVTPDHCDQFVSDESTAVIMRKVEEVAKQLNLRIAKKKERAIKLEGPHGVA 407

Query: 385 VAVIDIFRLTEELVVVEIKNRVREIDQDRQIWKDILRPQLDCLI 427
             V+ + RLT ELV+VE+KN+ R++     +W D LR +L  LI
Sbjct: 408 NVVVKVRRLTNELVMVEMKNKQRDVG---LVWADALRQKLRRLI 444

BLAST of Lsi01G016060 vs. TAIR10
Match: AT1G29230.1 (AT1G29230.1 CBL-interacting protein kinase 18)

HSP 1 Score: 443.7 bits (1140), Expect = 1.3e-124
Identity = 230/456 (50.44%), Postives = 297/456 (65.13%), Query Frame = 1

Query: 6   DDNPPPGGLTRGDSLPEGILFGKYELGKLLGCGAFAKVYHGRNVFTGQSVAIKAVNKLKV 65
           D N  P       S    IL GKYELGKLLG G FAKVY  +N+ +G  VAIK ++K K+
Sbjct: 51  DGNISPQSPRSPRSPRNNILMGKYELGKLLGHGTFAKVYLAQNIKSGDKVAIKVIDKEKI 110

Query: 66  LKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFAKVSKGRFSE 125
           +K G  AH+KREI+I+RR+RHP +V LFEV+ATK+KIYFVME+  GGELF  V+KGR  E
Sbjct: 111 MKSGLVAHIKREISILRRVRHPYIVHLFEVMATKSKIYFVMEYVGGGELFNTVAKGRLPE 170

Query: 126 NLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRPDGLL 185
             +RRYFQQLI++V +CH RG++HRDLKPENLLLD+  +L+VSDFGLSA+ +Q+R DGL 
Sbjct: 171 ETARRYFQQLISSVSFCHGRGVYHRDLKPENLLLDNKGNLKVSDFGLSAVAEQLRQDGLC 230

Query: 186 HTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGD 245
           HT CGTPAY+APE+L +KGYD AK DVWSCG+IL+VL  G++PF D N+M MY+KIY+G+
Sbjct: 231 HTFCGTPAYIAPEVLTRKGYDAAKADVWSCGVILFVLMAGHIPFYDKNIMVMYKKIYKGE 290

Query: 246 FHCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFVKGFQEEKF--------QQLED 305
           F CP+W S DL R L+RLLDTNP+TRIT+ EI ++ WF KGF+  KF        ++ ED
Sbjct: 291 FRCPRWFSSDLVRLLTRLLDTNPDTRITIPEIMKNRWFKKGFKHVKFYIEDDKLCREDED 350

Query: 306 SASEL----------------------YGDVNNAKGLNAFDLISFSPGFDLTGLMNETEF 365
              E                        G +     LNAFD+ISFS GFDL+GL  E E 
Sbjct: 351 EEEEASSSGRSSTVSESDAEFDVKRMGIGSMPRPSSLNAFDIISFSSGFDLSGLFEE-EG 410

Query: 366 SSGVERFLSAESPEKIIVTVEEVTKSENAAVEKNGWGAKV----EGLDGGFVAVIDIFRL 425
             G  RF+S     KII  +EE+ K  +  V K  W  ++    EG  G      +IF L
Sbjct: 411 GEGT-RFVSGAPVSKIISKLEEIAKIVSFTVRKKEWSLRLEGCREGAKGPLTIAAEIFEL 470

Query: 426 TEELVVVEIKNRVREIDQDRQIWKDILRPQLDCLIY 428
           T  LVVVE+K +  + ++  +     LRP+L+ LI+
Sbjct: 471 TPSLVVVEVKKKGGDREEYEEFCNKELRPELEKLIH 504

BLAST of Lsi01G016060 vs. TAIR10
Match: AT5G45810.1 (AT5G45810.1 CBL-interacting protein kinase 19)

HSP 1 Score: 438.0 bits (1125), Expect = 6.9e-123
Identity = 225/443 (50.79%), Postives = 295/443 (66.59%), Query Frame = 1

Query: 22  EGILFGKYELGKLLGCGAFAKVYHGRNVFTGQSVAIKAVNKLKVLKGGFTAHVKREIAIM 81
           + ++ GKYE+G+LLG G FAKVY  RN  +G+SVAIK ++K KVLK G  AH+KREI+I+
Sbjct: 21  QALILGKYEMGRLLGHGTFAKVYLARNAQSGESVAIKVIDKEKVLKSGLIAHIKREISIL 80

Query: 82  RRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFAKVSKGRFSENLSRRYFQQLITAVGY 141
           RR+RHPN+V+LFEV+ATK+KIYFVME+ KGGELF KV+KGR  E ++R+YFQQLI+AV +
Sbjct: 81  RRVRHPNIVQLFEVMATKSKIYFVMEYVKGGELFNKVAKGRLKEEMARKYFQQLISAVSF 140

Query: 142 CHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRPDGLLHTLCGTPAYVAPEILA 201
           CH RG++HRDLKPENLLLD+N +L+VSDFGLSA++DQIR DGL HT CGTPAYVAPE+LA
Sbjct: 141 CHFRGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTPAYVAPEVLA 200

Query: 202 KKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFHCPKWTSPDLRRFLS 261
           +KGYDGAK+D+WSCG+IL+VL  G+LPF+D N+MAMY+KIYRGDF CP+W   ++ R L 
Sbjct: 201 RKGYDGAKVDIWSCGVILFVLMAGFLPFHDRNVMAMYKKIYRGDFRCPRWFPVEINRLLI 260

Query: 262 RLLDTNPETRITVDEIHQDPWFVKGFQEEKF----------------------------- 321
           R+L+T PE R T+ +I +  WF KGF+  KF                             
Sbjct: 261 RMLETKPERRFTMPDIMETSWFKKGFKHIKFYVEDDHQLCNVADDDEIESIESVSGRSST 320

Query: 322 -QQLEDSAS----ELYGDVNNAKGLNAFDLISFSPGFDLTGLMNETEFSSGVERFLSAES 381
             + ED  S       G +     LNAFDLISFSPGFDL+GL  +    S   RF+S   
Sbjct: 321 VSEPEDFESFDGRRRGGSMPRPASLNAFDLISFSPGFDLSGLFEDDGEGS---RFVSGAP 380

Query: 382 PEKIIVTVEEVTKSENAAVEKNGWGAKVEG-----LDGGFVAVIDIFRLTEELVVVEIKN 426
             +II  +EE+ +  +  V K      +EG     + G      +IF LT  LVVVE+K 
Sbjct: 381 VGQIISKLEEIARIVSFTVRKKDCKVSLEGSREGSMKGPLSIAAEIFELTPALVVVEVKK 440

BLAST of Lsi01G016060 vs. NCBI nr
Match: gi|449464410|ref|XP_004149922.1| (PREDICTED: CBL-interacting serine/threonine-protein kinase 14-like [Cucumis sativus])

HSP 1 Score: 802.7 bits (2072), Expect = 3.0e-229
Identity = 400/433 (92.38%), Postives = 413/433 (95.38%), Query Frame = 1

Query: 1   MPAAFDDNPPPGGLTRGDSLPEGILFGKYELGKLLGCGAFAKVYHGRNVFTGQSVAIKAV 60
           MPA  DD PPPG L R DSLPEGILFGKYELGKLLGCGAFAKVYHGRNV TGQSVAIKAV
Sbjct: 1   MPAFHDDTPPPG-LPRADSLPEGILFGKYELGKLLGCGAFAKVYHGRNVLTGQSVAIKAV 60

Query: 61  NKLKVLKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFAKVSK 120
           NKLKVLKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATKTKIYFVME+AKGGELF KVSK
Sbjct: 61  NKLKVLKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATKTKIYFVMEYAKGGELFGKVSK 120

Query: 121 GRFSENLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIR 180
           GRFSENLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSAL DQIR
Sbjct: 121 GRFSENLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSALADQIR 180

Query: 181 PDGLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRK 240
           PDGLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLT GYLPFNDSNLMAMYRK
Sbjct: 181 PDGLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTAGYLPFNDSNLMAMYRK 240

Query: 241 IYRGDFHCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFVKGFQEEK--FQQLEDS 300
           IYRGDF CPKWTSPDL+RF+SRLLDTNPE+RITVDEI +DPWFVKGFQEEK  FQ+L+DS
Sbjct: 241 IYRGDFRCPKWTSPDLQRFISRLLDTNPESRITVDEIQRDPWFVKGFQEEKFQFQELDDS 300

Query: 301 ASELYGDVNNAKGLNAFDLISFSPGFDLTGLM-NETEFSSGVERFLSAESPEKIIVTVEE 360
           AS++YGDVNNAK LNAFDLISFSPGFDLTGL+  ETEFSS VERFLSAESPEKIIVTVEE
Sbjct: 301 ASDVYGDVNNAKCLNAFDLISFSPGFDLTGLIKEETEFSSCVERFLSAESPEKIIVTVEE 360

Query: 361 VTKSENAAVEKNGWGAKVEGLDGGFVAVIDIFRLTEELVVVEIKNRVREIDQDRQIWKDI 420
           VTKSENAAVEKNGWG KVEGLDGGFV VIDIFRLTEELVVVEIKNRVREI+QDRQIWKD+
Sbjct: 361 VTKSENAAVEKNGWGMKVEGLDGGFVVVIDIFRLTEELVVVEIKNRVREIEQDRQIWKDV 420

Query: 421 LRPQLDCLIYRSD 431
           LRPQL+CLIYRSD
Sbjct: 421 LRPQLECLIYRSD 432

BLAST of Lsi01G016060 vs. NCBI nr
Match: gi|659128564|ref|XP_008464265.1| (PREDICTED: CBL-interacting serine/threonine-protein kinase 14 [Cucumis melo])

HSP 1 Score: 789.6 bits (2038), Expect = 2.7e-225
Identity = 395/434 (91.01%), Postives = 406/434 (93.55%), Query Frame = 1

Query: 1   MPAAFDDNPPPGGLTRGDSLPEGILFGKYELGKLLGCGAFAKVYHGRNVFTGQSVAIKAV 60
           MPA  DD PPPG L R DSLPEGILFGKYELGKLLGCGAFAKVYHGRNV TGQSVAIKAV
Sbjct: 1   MPAFHDDTPPPG-LPRADSLPEGILFGKYELGKLLGCGAFAKVYHGRNVLTGQSVAIKAV 60

Query: 61  NKLKVLKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFAKVSK 120
           NKLKVLKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATKTKIYFVME+AKGGELF KVSK
Sbjct: 61  NKLKVLKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATKTKIYFVMEYAKGGELFGKVSK 120

Query: 121 GRFSENLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIR 180
           GRFSENLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSAL DQIR
Sbjct: 121 GRFSENLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSALADQIR 180

Query: 181 PDGLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRK 240
           PDGLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLT GYLPFNDSNLM+MYRK
Sbjct: 181 PDGLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTAGYLPFNDSNLMSMYRK 240

Query: 241 IYRGDFHCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFVKGFQEEKFQ---QLED 300
           IYRGDF CPKWTSPDL+RFLSRLLDTNPETRITVDEI +DPWFVKGFQEEKFQ    +ED
Sbjct: 241 IYRGDFRCPKWTSPDLQRFLSRLLDTNPETRITVDEIQRDPWFVKGFQEEKFQFQELIED 300

Query: 301 SASELYGDVNNAKGLNAFDLISFSPGFDLTGLMNETEFSSGVERFLSAESPEKIIVTVEE 360
            ASE+YGDVNN+K LNAFDLISFSPGFDLTGL+ ETEFSS VERFLSAESPEKII  VEE
Sbjct: 301 PASEVYGDVNNSKYLNAFDLISFSPGFDLTGLIKETEFSSCVERFLSAESPEKIITRVEE 360

Query: 361 VTKSENAAVEKNGWGAKVEGLDGGFVAVIDIFRLTEELVVVEIKNRVREIDQD-RQIWKD 420
           VT+SENAAVEKNGWG KVEGLDGGFV VIDIFRLTEELVVVEIKNRVRE DQD  Q WKD
Sbjct: 361 VTESENAAVEKNGWGVKVEGLDGGFVVVIDIFRLTEELVVVEIKNRVRETDQDGDQRWKD 420

Query: 421 ILRPQLDCLIYRSD 431
           +LRPQL+CLIYRSD
Sbjct: 421 VLRPQLECLIYRSD 433

BLAST of Lsi01G016060 vs. NCBI nr
Match: gi|595824284|ref|XP_007205214.1| (hypothetical protein PRUPE_ppa005902mg [Prunus persica])

HSP 1 Score: 614.4 bits (1583), Expect = 1.5e-172
Identity = 299/407 (73.46%), Postives = 351/407 (86.24%), Query Frame = 1

Query: 25  LFGKYELGKLLGCGAFAKVYHGRNVFTGQSVAIKAVNKLKVLKGGFTAHVKREIAIMRRL 84
           LFGKYELG+LLG GAFAKVYH RNV TGQSVAIKAV+K KVLKGGFTAHVKREI+IMRRL
Sbjct: 27  LFGKYELGRLLGRGAFAKVYHARNVDTGQSVAIKAVSKQKVLKGGFTAHVKREISIMRRL 86

Query: 85  RHPNVVRLFEVLATKTKIYFVMEFAKGGELFAKVSKGRFSENLSRRYFQQLITAVGYCHS 144
           RHP++V+L+EVLATKTKIYF+MEFAKGGELFAK+SKGRFSE+LSRRYFQQLI+A+GYCHS
Sbjct: 87  RHPHIVKLYEVLATKTKIYFIMEFAKGGELFAKISKGRFSEDLSRRYFQQLISAIGYCHS 146

Query: 145 RGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRPDGLLHTLCGTPAYVAPEILAKKG 204
           RG+FHRDLKPENLLLD+N++L+VSDFGLSA+T+Q+RPDGLLHTLCGTPAYVAPEIL KKG
Sbjct: 147 RGVFHRDLKPENLLLDENWNLKVSDFGLSAVTEQVRPDGLLHTLCGTPAYVAPEILGKKG 206

Query: 205 YDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFHCPKWTSPDLRRFLSRLL 264
           Y+GAK+D+WSCGIIL+VL +GYLPFND NLM MYRKIY+GDF  P+WTSPDLRR +S LL
Sbjct: 207 YEGAKVDIWSCGIILFVLNSGYLPFNDQNLMVMYRKIYKGDFRFPRWTSPDLRRLISSLL 266

Query: 265 DTNPETRITVDEIHQDPWFVKGFQEEKFQQLEDSASELYGDVNNAKGLNAFDLISFSPGF 324
           DTNPETRITVDEI +DPWF KG++E KF   ED   +   D +N   LNAFDLISFS GF
Sbjct: 267 DTNPETRITVDEILKDPWFGKGYEEIKF-HFEDFELKDLRDGDNDTSLNAFDLISFSSGF 326

Query: 325 DLTGLMNETEFSSGVERFLSAESPEKIIVTVEEVTKSENAAV-EKNGWGAKVEGLDGGFV 384
           D++GL +++E S   ERF+SAE+PE II  VEE+ K+EN AV EK  WGAK+ G +G FV
Sbjct: 327 DISGLFSKSEISDCGERFISAETPETIIEKVEEIAKAENVAVMEKKSWGAKLAGQNGNFV 386

Query: 385 AVIDIFRLTEELVVVEIKNRVREIDQDRQIWKDILRPQLDCLIYRSD 431
            +I I+RLTEELVVVE+  R R+ +  ++IWKD LRPQL CLIYR +
Sbjct: 387 VMIGIYRLTEELVVVELNLRQRQSECSQRIWKDKLRPQLSCLIYRPE 432

BLAST of Lsi01G016060 vs. NCBI nr
Match: gi|965434012|gb|ALT16768.1| (CBL-interacting serine/threonine-protein kinase 11 [Malus domestica])

HSP 1 Score: 610.5 bits (1573), Expect = 2.2e-171
Identity = 298/416 (71.63%), Postives = 353/416 (84.86%), Query Frame = 1

Query: 17  GDSLPEGILFGKYELGKLLGCGAFAKVYHGRNVFTGQSVAIKAVNKLKVLKGGFTAHVKR 76
           G S  E  LFGKYE+G+LLGCGAFAKVYH RNV TGQSVAIKAV+K KV+K GFTAHVKR
Sbjct: 13  GSSAGEVNLFGKYEVGRLLGCGAFAKVYHARNVHTGQSVAIKAVSKQKVVKDGFTAHVKR 72

Query: 77  EIAIMRRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFAKVSKGRFSENLSRRYFQQLI 136
           EI+IMRRLRHP++V+LFEVLATKTKIYF+MEFAKGGELFAK+SKGRFSE+LSRRYFQQLI
Sbjct: 73  EISIMRRLRHPHIVKLFEVLATKTKIYFIMEFAKGGELFAKISKGRFSEDLSRRYFQQLI 132

Query: 137 TAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRPDGLLHTLCGTPAYVA 196
           +AVGYCHSRG+FHRDLKPENLLLD+N++L+VSDFGLSA+T+Q+RPDGLL+TLCGTPAYVA
Sbjct: 133 SAVGYCHSRGVFHRDLKPENLLLDENWNLKVSDFGLSAVTEQVRPDGLLYTLCGTPAYVA 192

Query: 197 PEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFHCPKWTSPDL 256
           PEILAKKGYDGAKID+WSCGI+L+VL  GYLPFND NLM MYRKIY+GD+  PKWTSPDL
Sbjct: 193 PEILAKKGYDGAKIDIWSCGIVLFVLNAGYLPFNDPNLMVMYRKIYKGDYRVPKWTSPDL 252

Query: 257 RRFLSRLLDTNPETRITVDEIHQDPWFVKGFQEEKFQQLEDSASELY-GDVNNAKGLNAF 316
           +R +SRLLDTN ETRITVDEI +DPWF KG+QE KF   +    ++  GD +    LNAF
Sbjct: 253 KRLISRLLDTNSETRITVDEILKDPWFAKGYQEIKFHFEDFDLKDMRDGDSDETSSLNAF 312

Query: 317 DLISFSPGFDLTGLMNETEFSSGVERFLSAESPEKIIVTVEEVTKSENAAV-EKNGWGAK 376
           DLISFS GFD++GL +++  S   ERF+SAE+PE+I+  VEEV K+ + AV EK  WGAK
Sbjct: 313 DLISFSSGFDISGLFSKSGISDCGERFVSAETPERIMEKVEEVAKAGDMAVTEKKSWGAK 372

Query: 377 VEGLDGGFVAVIDIFRLTEELVVVEIKNRVREIDQDRQIWKDILRPQLDCLIYRSD 431
           + G +G FV +I I+RLTE+LVVVE+K R RE +   QIWKD LRP+L CL+YR +
Sbjct: 373 LAGQNGNFVVMIGIYRLTEKLVVVELKLRQREAECGPQIWKDKLRPELSCLMYRPE 428

BLAST of Lsi01G016060 vs. NCBI nr
Match: gi|657957485|ref|XP_008370243.1| (PREDICTED: CBL-interacting serine/threonine-protein kinase 14-like [Malus domestica])

HSP 1 Score: 610.5 bits (1573), Expect = 2.2e-171
Identity = 298/416 (71.63%), Postives = 353/416 (84.86%), Query Frame = 1

Query: 17  GDSLPEGILFGKYELGKLLGCGAFAKVYHGRNVFTGQSVAIKAVNKLKVLKGGFTAHVKR 76
           G S  E  LFGKYE+G+LLGCGAFAKVYH RNV TGQSVAIKAV+K KV+K GFTAHVKR
Sbjct: 13  GSSAGEVNLFGKYEVGRLLGCGAFAKVYHARNVHTGQSVAIKAVSKQKVVKDGFTAHVKR 72

Query: 77  EIAIMRRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFAKVSKGRFSENLSRRYFQQLI 136
           EI+IMRRLRHP++V+LFEVLATKTKIYF+MEFAKGGELFAK+SKGRFSE+LSRRYFQQLI
Sbjct: 73  EISIMRRLRHPHIVKLFEVLATKTKIYFIMEFAKGGELFAKISKGRFSEDLSRRYFQQLI 132

Query: 137 TAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRPDGLLHTLCGTPAYVA 196
           +AVGYCHSRG+FHRDLKPENLLLD+N++L+VSDFGLSA+T+Q+RPDGLL+TLCGTPAYVA
Sbjct: 133 SAVGYCHSRGVFHRDLKPENLLLDENWNLKVSDFGLSAVTEQVRPDGLLYTLCGTPAYVA 192

Query: 197 PEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFHCPKWTSPDL 256
           PEILAKKGYDGAKID+WSCGI+L+VL  GYLPFND NLM MYRKIY+GD+  PKWTSPDL
Sbjct: 193 PEILAKKGYDGAKIDIWSCGIVLFVLNAGYLPFNDPNLMVMYRKIYKGDYRVPKWTSPDL 252

Query: 257 RRFLSRLLDTNPETRITVDEIHQDPWFVKGFQEEKFQQLEDSASELY-GDVNNAKGLNAF 316
           +R +SRLLDTN ETRITVDEI +DPWF KG+QE KF   +    ++  GD +    LNAF
Sbjct: 253 KRLISRLLDTNSETRITVDEILKDPWFAKGYQEIKFHFEDFDLKDMRDGDSDETSSLNAF 312

Query: 317 DLISFSPGFDLTGLMNETEFSSGVERFLSAESPEKIIVTVEEVTKSENAAV-EKNGWGAK 376
           DLISFS GFD++GL +++  S   ERF+SAE+PE+I+  VEEV K+ + AV EK  WGAK
Sbjct: 313 DLISFSSGFDISGLFSKSGISDCGERFVSAETPERIMEKVEEVAKAGDMAVTEKKSWGAK 372

Query: 377 VEGLDGGFVAVIDIFRLTEELVVVEIKNRVREIDQDRQIWKDILRPQLDCLIYRSD 431
           + G +G FV +I I+RLTE+LVVVE+K R RE +   QIWKD LRP+L CL+YR +
Sbjct: 373 LAGQNGNFVVMIGIYRLTEKLVVVELKLRQREAECGPQIWKDKLRPELSCLMYRPE 428

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CIPKE_ARATH2.8e-15865.70CBL-interacting serine/threonine-protein kinase 14 OS=Arabidopsis thaliana GN=CI... [more]
CIPKB_ARATH1.1e-13862.47CBL-interacting serine/threonine-protein kinase 11 OS=Arabidopsis thaliana GN=CI... [more]
CIPKM_ARATH4.5e-12457.43CBL-interacting serine/threonine-protein kinase 22 OS=Arabidopsis thaliana GN=CI... [more]
CIPKI_ARATH2.2e-12350.44CBL-interacting serine/threonine-protein kinase 18 OS=Arabidopsis thaliana GN=CI... [more]
CIPKJ_ARATH1.2e-12150.79CBL-interacting serine/threonine-protein kinase 19 OS=Arabidopsis thaliana GN=CI... [more]
Match NameE-valueIdentityDescription
A0A0A0L2V2_CUCSA2.1e-22992.38Non-specific serine/threonine protein kinase OS=Cucumis sativus GN=Csa_4G331590 ... [more]
M5WAX9_PRUPE1.1e-17273.46Non-specific serine/threonine protein kinase OS=Prunus persica GN=PRUPE_ppa00590... [more]
A0A0U3BTT2_MALDO1.5e-17171.63Non-specific serine/threonine protein kinase OS=Malus domestica GN=CIPK11 PE=2 S... [more]
W9QMI1_9ROSA2.1e-16872.40Non-specific serine/threonine protein kinase OS=Morus notabilis GN=L484_021953 P... [more]
A0A067JG26_JATCU6.7e-16772.75Non-specific serine/threonine protein kinase OS=Jatropha curcas GN=JCGZ_00369 PE... [more]
Match NameE-valueIdentityDescription
AT5G01820.11.6e-15965.70 serine/threonine protein kinase 1[more]
AT2G30360.16.2e-14062.47 SOS3-interacting protein 4[more]
AT2G38490.12.5e-12557.43 CBL-interacting protein kinase 22[more]
AT1G29230.11.3e-12450.44 CBL-interacting protein kinase 18[more]
AT5G45810.16.9e-12350.79 CBL-interacting protein kinase 19[more]
Match NameE-valueIdentityDescription
gi|449464410|ref|XP_004149922.1|3.0e-22992.38PREDICTED: CBL-interacting serine/threonine-protein kinase 14-like [Cucumis sati... [more]
gi|659128564|ref|XP_008464265.1|2.7e-22591.01PREDICTED: CBL-interacting serine/threonine-protein kinase 14 [Cucumis melo][more]
gi|595824284|ref|XP_007205214.1|1.5e-17273.46hypothetical protein PRUPE_ppa005902mg [Prunus persica][more]
gi|965434012|gb|ALT16768.1|2.2e-17171.63CBL-interacting serine/threonine-protein kinase 11 [Malus domestica][more]
gi|657957485|ref|XP_008370243.1|2.2e-17171.63PREDICTED: CBL-interacting serine/threonine-protein kinase 14-like [Malus domest... [more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0007165signal transduction
GO:0006468protein phosphorylation
Vocabulary: Molecular Function
TermDefinition
GO:0005524ATP binding
GO:0004672protein kinase activity
Vocabulary: INTERPRO
TermDefinition
IPR020636Ca/CaM-dep_Ca-dep_prot_Kinase
IPR018451NAF/FISL_domain
IPR017441Protein_kinase_ATP_BS
IPR011009Kinase-like_dom_sf
IPR008271Ser/Thr_kinase_AS
IPR004041NAF_dom
IPR000719Prot_kinase_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0009069 serine family amino acid metabolic process
biological_process GO:0007165 signal transduction
cellular_component GO:0009506 plasmodesma
cellular_component GO:0005773 vacuole
cellular_component GO:0005575 cellular_component
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi01G016060.1Lsi01G016060.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 29..283
score: 1.8
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 29..283
score: 1.8E
IPR000719Protein kinase domainPROFILEPS50011PROTEIN_KINASE_DOMcoord: 29..283
score: 51
IPR004041NAF domainPFAMPF03822NAFcoord: 312..368
score: 6.3
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 147..159
scor
IPR011009Protein kinase-like domainunknownSSF56112Protein kinase-like (PK-like)coord: 25..296
score: 1.89
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 35..58
scor
IPR018451NAF/FISL domainPROFILEPS50816NAFcoord: 307..331
score: 1
IPR020636Calcium/calmodulin-dependent/calcium-dependent protein kinasePANTHERPTHR24347SERINE/THREONINE-PROTEIN KINASEcoord: 25..430
score: 8.3E
NoneNo IPR availableGENE3DG3DSA:1.10.510.10coord: 95..293
score: 9.5
NoneNo IPR availableGENE3DG3DSA:3.30.200.20coord: 24..94
score: 3.6
NoneNo IPR availablePANTHERPTHR24347:SF189SUBFAMILY NOT NAMEDcoord: 25..430
score: 8.3E

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi01G016060Wax gourdlsiwgoB151
Lsi01G016060Bottle gourd (USVL1VR-Ls)lsilsiB071
Lsi01G016060Bottle gourd (USVL1VR-Ls)lsilsiB072
Lsi01G016060Cucumber (Gy14) v1cgylsiB293
Lsi01G016060Cucurbita maxima (Rimu)cmalsiB231
Lsi01G016060Cucurbita maxima (Rimu)cmalsiB270
Lsi01G016060Cucurbita maxima (Rimu)cmalsiB406
Lsi01G016060Cucurbita maxima (Rimu)cmalsiB641
Lsi01G016060Cucurbita maxima (Rimu)cmalsiB748
Lsi01G016060Cucurbita moschata (Rifu)cmolsiB219
Lsi01G016060Cucurbita moschata (Rifu)cmolsiB259
Lsi01G016060Cucurbita moschata (Rifu)cmolsiB391
Lsi01G016060Cucurbita moschata (Rifu)cmolsiB629
Lsi01G016060Cucurbita moschata (Rifu)cmolsiB741
Lsi01G016060Cucurbita pepo (Zucchini)cpelsiB328
Lsi01G016060Cucurbita pepo (Zucchini)cpelsiB492
Lsi01G016060Cucurbita pepo (Zucchini)cpelsiB703
Lsi01G016060Wild cucumber (PI 183967)cpilsiB168
Lsi01G016060Cucumber (Chinese Long) v2culsiB169
Lsi01G016060Melon (DHL92) v3.5.1lsimeB141
Lsi01G016060Watermelon (Charleston Gray)lsiwcgB119
Lsi01G016060Watermelon (Charleston Gray)lsiwcgB124
Lsi01G016060Watermelon (97103) v1lsiwmB128
Lsi01G016060Cucumber (Gy14) v2cgyblsiB154
Lsi01G016060Melon (DHL92) v3.6.1lsimedB147
Lsi01G016060Silver-seed gourdcarlsiB015
Lsi01G016060Silver-seed gourdcarlsiB139
Lsi01G016060Silver-seed gourdcarlsiB231
Lsi01G016060Silver-seed gourdcarlsiB571
Lsi01G016060Silver-seed gourdcarlsiB911
Lsi01G016060Cucumber (Chinese Long) v3cuclsiB186
Lsi01G016060Cucumber (Chinese Long) v3cuclsiB272
Lsi01G016060Watermelon (97103) v2lsiwmbB117
Lsi01G016060Watermelon (97103) v2lsiwmbB122
Lsi01G016060Watermelon (97103) v2lsiwmbB140