Lsi01G014540 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi01G014540
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Putative reductase 1
Location	chr01 : 12668280 .. 12669416 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCCATTGGCCATTTCGAATGAAACATGGATCTATTGGCTTTTCTCCTGAAAACTTGGTTGCGCCAGACATACCGAGCACATGGAGAGCCATGGAAGCTCTGTTTGACTCTGGAAAAGCTCGAGCCATTGGTGTTAGCAATTTCACCATCAACAAACTTAGAGATTTGCTCGAGATAGCTCGCATTCGTCCCGCTGTCAATGAGGTGGAGTCTCACCCATCATGGCAGCAAGACAAACTACGTGAATATTGCAAATCAAAAGAAATTCATCTAAGTGTGAGTGTAGAAAAAAAAAACTTAGGGAGCACCTATCTTTATGCATTCTATCAAATTATCGCATCATAATTCTTTTTATGTGAGTGATGAAAATGAGATGCATCAAGATGGGTGTTACTCAGGATGCACCCAAGTATTACTCATATCATTCAAGTATTACTCATATCATTATAGCCTGACACTTCGACTCGAATTTCAGGCATACTTTCCATTGGGTTCACGTGGGTCAACTTGGTTCAAGAATGATGATATTCTTCAAAATCCAATTCTTAAAGAGATTGCTGAAAAAATTGGTAAGACTCCTGCACAAGTTGCCCTTCGCTGGGGACTACAAAAGGGACATAGTGTGCTTCCAAAAAGCACTACTGAGTCAAGGATCAAAGAGAATTTCGATATTTTCGATTGGTCCATACCTGAAGATTTGTTTGCTAAGTTTTCTCAGATCGATCAGGTAAGTAAATATAGTTACTTATTTTGATTTTTTAGAAACGTAAAACAAAAACACAATTATCAATTATATTTTTTATTTTTAGTTTAAAAAACAGAACATAAAAAATAATTACTAAACATGTTTTTGATTTTTTGTTTTTAAAAATTAGAAAACCAAAAACAGTAACTAAACATATATGATTTTTGTTTTTTTATTTTTAAATTAAAAATAAAAAACCAAATATTTATCAAATGAGACGTTAATGATGCATGATTTTAGCCGTATCTACGAAATTGTTATTACGTTAATTAGTATACTAAAATTCACTCCGTTCTTCACTTGGACAGGCAAGGATTGTGTTAGGAGCCATGTTTACGAGTGAGAACGGTTTGTATCAAATGGTTGAGGAATTGTGGGATGGAGAGATCTAA

mRNA sequence

ATCCATTGGCCATTTCGAATGAAACATGGATCTATTGGCTTTTCTCCTGAAAACTTGGTTGCGCCAGACATACCGAGCACATGGAGAGCCATGGAAGCTCTGTTTGACTCTGGAAAAGCTCGAGCCATTGGTGTTAGCAATTTCACCATCAACAAACTTAGAGATTTGCTCGAGATAGCTCGCATTCGTCCCGCTGTCAATGAGGCATACTTTCCATTGGGTTCACGTGGGTCAACTTGGTTCAAGAATGATGATATTCTTCAAAATCCAATTCTTAAAGAGATTGCTGAAAAAATTGGTAAGACTCCTGCACAAGTTGCCCTTCGCTGGGGACTACAAAAGGGACATAGTGCAAGGATTGTGTTAGGAGCCATGTTTACGAGTGAGAACGGTTTGTATCAAATGGTTGAGGAATTGTGGGATGGAGAGATCTAA

Coding sequence (CDS)

ATCCATTGGCCATTTCGAATGAAACATGGATCTATTGGCTTTTCTCCTGAAAACTTGGTTGCGCCAGACATACCGAGCACATGGAGAGCCATGGAAGCTCTGTTTGACTCTGGAAAAGCTCGAGCCATTGGTGTTAGCAATTTCACCATCAACAAACTTAGAGATTTGCTCGAGATAGCTCGCATTCGTCCCGCTGTCAATGAGGCATACTTTCCATTGGGTTCACGTGGGTCAACTTGGTTCAAGAATGATGATATTCTTCAAAATCCAATTCTTAAAGAGATTGCTGAAAAAATTGGTAAGACTCCTGCACAAGTTGCCCTTCGCTGGGGACTACAAAAGGGACATAGTGCAAGGATTGTGTTAGGAGCCATGTTTACGAGTGAGAACGGTTTGTATCAAATGGTTGAGGAATTGTGGGATGGAGAGATCTAA

Protein sequence

IHWPFRMKHGSIGFSPENLVAPDIPSTWRAMEALFDSGKARAIGVSNFTINKLRDLLEIARIRPAVNEAYFPLGSRGSTWFKNDDILQNPILKEIAEKIGKTPAQVALRWGLQKGHSARIVLGAMFTSENGLYQMVEELWDGEI

Homology

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BLAST of Lsi01G014540 vs. Swiss-Prot
Match: AKRC9_ARATH (Aldo-keto reductase family 4 member C9 OS=Arabidopsis thaliana GN=AKR4C9 PE=1 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 1.2e-44
Identity = 98/204 (48.04%), Postives = 118/204 (57.84%), Query Frame = 1


Query: 1   IHWPFRMKHGSIGFSPENLVAPDIPSTWRAMEALFDSGKARAIGVSNFTINKLRDLLEIA 60
           IHWP R+K GS+G  PENL+  DIPSTW+AMEAL+DSGKARAIGVSNF+  KL DLLE+A
Sbjct: 113 IHWPARIKKGSVGIKPENLLPVDIPSTWKAMEALYDSGKARAIGVSNFSTKKLADLLELA 172

Query: 61  RIRPAVNE------------------------AYFPLGSRGSTWFKNDDILQNPILKEIA 120
           R+ PAVN+                        AY PLGS G+TW K+D +L+NPIL  +A
Sbjct: 173 RVPPAVNQVECHPSWRQTKLQEFCKSKGVHLSAYSPLGSPGTTWLKSD-VLKNPILNMVA 232

Query: 121 EKIGKTPAQVALRWGLQKGHS-----------------------------------ARIV 145
           EK+GK+PAQVALRWGLQ GHS                                   AR+V
Sbjct: 233 EKLGKSPAQVALRWGLQMGHSVLPKSTNEGRIKENFNVFDWSIPDYMFAKFAEIEQARLV 292

[Close]

BLAST of Lsi01G014540 vs. Swiss-Prot
Match: AKRCA_ARATH (Aldo-keto reductase family 4 member C10 OS=Arabidopsis thaliana GN=AKR4C10 PE=1 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 1.4e-42
Identity = 94/203 (46.31%), Postives = 112/203 (55.17%), Query Frame = 1


Query: 1   IHWPFRMKHGSIGFSPENLVAPDIPSTWRAMEALFDSGKARAIGVSNFTINKLRDLLEIA 60
           IHWP  +K GS GF PEN++  DIPSTW+AME+LFDSGKARAIGVSNF+  KL DLL +A
Sbjct: 113 IHWPVSLKKGSTGFKPENILPTDIPSTWKAMESLFDSGKARAIGVSNFSSKKLADLLVVA 172

Query: 61  RIRPAVNEA------------------------YFPLGSRGSTWFKNDDILQNPILKEIA 120
           R+ PAVN+                         Y PLGS G+TW  +D +L+NPIL  +A
Sbjct: 173 RVPPAVNQVECHPSWQQNVLRDFCKSKGVHLSGYSPLGSPGTTWLTSD-VLKNPILGGVA 232

Query: 121 EKIGKTPAQVALRWGLQKGHS-----------------------------------ARIV 145
           EK+GKTPAQVALRWGLQ G S                                    R+V
Sbjct: 233 EKLGKTPAQVALRWGLQMGQSVLPKSTHEDRIKQNFDVFNWSIPEDMLSKFSEIGQGRLV 292

[Close]

BLAST of Lsi01G014540 vs. Swiss-Prot
Match: AKRCB_ARATH (Aldo-keto reductase family 4 member C11 OS=Arabidopsis thaliana GN=AKR4C11 PE=1 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 5.9e-36
Identity = 86/204 (42.16%), Postives = 111/204 (54.41%), Query Frame = 1


Query: 1   IHWPFRMKHGSIGFSPENLVAPDIPSTWRAMEALFDSGKARAIGVSNFTINKLRDLLEIA 60
           +HWP R+K G++ F PEN++  DIPSTW+AMEAL DSGKARAIGVSNF+  KL DL+E A
Sbjct: 113 MHWPVRLKKGTVDFKPENIMPIDIPSTWKAMEALVDSGKARAIGVSNFSTKKLSDLVEAA 172

Query: 61  RIRPAVNEA-YFPLGSR-----------------------GSTWFKNDDILQNPILKEIA 120
           R+ PAVN+    P   +                       G+TW K  D+L++P+++ IA
Sbjct: 173 RVPPAVNQVECHPSWQQHKLHEFCKSKGIHLSGYSPLGSPGTTWVK-ADVLKSPVIEMIA 232

Query: 121 EKIGKTPAQVALRWGLQKGHS-----------------------------------ARIV 145
           ++IGK+PAQ ALRWGLQ GHS                                   AR+V
Sbjct: 233 KEIGKSPAQTALRWGLQMGHSILPKSTNEGRIRENFDVLGWSIPKEMFDKFSKIEQARLV 292

[Close]

BLAST of Lsi01G014540 vs. Swiss-Prot
Match: AKRC8_ARATH (Aldo-keto reductase family 4 member C8 OS=Arabidopsis thaliana GN=AKR4C8 PE=1 SV=2)

HSP 1 Score: 148.3 bits (373), Expect = 6.5e-35
Identity = 90/206 (43.69%), Postives = 110/206 (53.40%), Query Frame = 1


Query: 1   IHWPFRMKHGSIGFSPENLVAPDIPSTWRAMEALFDSGKARAIGVSNFTINKLRDLLEIA 60
           IHWP  +K  S+  +PE L  PDI STW+AMEAL+DSGKARAIGVSNF+  KL DLL +A
Sbjct: 109 IHWPASLKKESLMPTPEMLTKPDITSTWKAMEALYDSGKARAIGVSNFSSKKLTDLLNVA 168

Query: 61  RIRPAVNEA------------------------YFPLGS--RGSTWFKNDDILQNPILKE 120
           R+ PAVN+                         Y PLGS  +G    K   +LQNPI+ E
Sbjct: 169 RVTPAVNQVECHPVWQQQGLHELCKSKGVHLSGYSPLGSQSKGEVRLK---VLQNPIVTE 228

Query: 121 IAEKIGKTPAQVALRWGLQKGHS--------ARI----------VLGAMFT--------- 145
           +AEK+GKT AQVALRWGLQ GHS        AR+          +   +FT         
Sbjct: 229 VAEKLGKTTAQVALRWGLQTGHSVLPKSSSGARLKENLDVFDWSIPEDLFTKFSNIPQEK 288

[Close]

BLAST of Lsi01G014540 vs. Swiss-Prot
Match: GLD2_HYPJE (Glycerol 2-dehydrogenase (NADP(+)) OS=Hypocrea jecorina GN=gld2 PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 6.5e-19
Identity = 55/149 (36.91%), Postives = 77/149 (51.68%), Query Frame = 1


Query: 1   IHWPFRMKHGS---IGFSPE-----NLVAPDIPS-TWRAMEALFDSGKARAIGVSNFTIN 60
           +HWP   +  S   +   P+     N    + P  TWRAME L +SG  +AIGVSN+TI 
Sbjct: 114 VHWPIAAEKNSDRSVKLGPDGKYVINQALTENPEPTWRAMEELVESGLVKAIGVSNWTIP 173

Query: 61  KLRDLLEIARIRPAVN------------------------EAYFPLGSRGSTWFKNDDIL 117
            L+ LL+IA+I+PAVN                        EAY PLGS+       + + 
Sbjct: 174 GLKKLLQIAKIKPAVNQIEIHPFLPNEELVAFCFENGILPEAYSPLGSQNQVPSTGERVR 233

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BLAST of Lsi01G014540 vs. TrEMBL
Match: A0A0A0L2B3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G296830 PE=4 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 3.6e-48
Identity = 96/141 (68.09%), Postives = 105/141 (74.47%), Query Frame = 1


Query: 1   IHWPFRMKHGSIGFSPENLVAPDIPSTWRAMEALFDSGKARAIGVSNFTINKLRDLLEIA 60
           IHWP RMKHGS GF+PEN +APDIPSTWRAMEALFDSGKARAIGVSNF+  KL DLLE+A
Sbjct: 113 IHWPVRMKHGSTGFAPENFLAPDIPSTWRAMEALFDSGKARAIGVSNFSSKKLGDLLEVA 172

Query: 61  RIRPAVNEA------------------------YFPLGSRGSTWFKNDDILQNPILKEIA 118
           R+ PAVN+                         Y PLGSRG+TW K  DIL+NPILKEIA
Sbjct: 173 RVPPAVNQVECHLSWQQDKLREFCKSKGVHLTGYSPLGSRGTTWLKGGDILENPILKEIA 232

[Close]

BLAST of Lsi01G014540 vs. TrEMBL
Match: W9QFW8_9ROSA (Aldo-keto reductase family 4 member C9 OS=Morus notabilis GN=L484_016975 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 4.4e-46
Identity = 93/169 (55.03%), Postives = 116/169 (68.64%), Query Frame = 1


Query: 1   IHWPFRMKHGSIGFSPENLVAPDIPSTWRAMEALFDSGKARAIGVSNFTINKLRDLLEIA 60
           IHWP RMK GS+GF PENL   DIP TW AMEAL+D+GKA+AIGVSNF+  KLRD+L++A
Sbjct: 113 IHWPVRMKKGSVGFKPENLDQLDIPGTWSAMEALYDAGKAKAIGVSNFSTKKLRDMLDVA 172

Query: 61  RIRPAVNE------------------------AYFPLGSRGSTWFKNDDILQNPILKEIA 120
            + PAVN+                        AY PLGS G+TW K+ D+LQNPI+  +A
Sbjct: 173 CVPPAVNQVECHPSWRQDKLHALCRSKGVHLSAYSPLGSPGTTWIKS-DVLQNPIVNMVA 232

Query: 121 EKIGKTPAQVALRWGLQKGHSARIVLGAMFTSEN-GLYQMVEELWDGEI 145
           EK+GKTPAQVALRWGLQ GHSAR++ G  F  +  G Y+ +E+ WDGE+
Sbjct: 233 EKLGKTPAQVALRWGLQMGHSARLLRGTSFVHDTYGPYRTLEDFWDGEL 280

[Close]

BLAST of Lsi01G014540 vs. TrEMBL
Match: A0A0A0L110_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G296840 PE=4 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 8.3e-45
Identity = 90/141 (63.83%), Postives = 101/141 (71.63%), Query Frame = 1


Query: 1   IHWPFRMKHGSIGFSPENLVAPDIPSTWRAMEALFDSGKARAIGVSNFTINKLRDLLEIA 60
           IHWP R+KHGS   +PEN VAPDIPSTW+AME L+DSGKARAIGVSN +  KL DLLE+A
Sbjct: 115 IHWPVRLKHGSTDLAPENFVAPDIPSTWKAMEELYDSGKARAIGVSNLSTKKLEDLLEVA 174

Query: 61  RIRPAVNEA------------------------YFPLGSRGSTWFKNDDILQNPILKEIA 118
           RI PAVN+                         Y PLGSRG+TW K  DIL+NPILKEIA
Sbjct: 175 RITPAVNQVECHPGWQQNKLHEFCESKGIHLSGYSPLGSRGTTWLKGGDILENPILKEIA 234

[Close]

BLAST of Lsi01G014540 vs. TrEMBL
Match: W9QNA5_9ROSA (Aldo-keto reductase family 4 member C9 OS=Morus notabilis GN=L484_016978 PE=4 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 4.1e-44
Identity = 102/204 (50.00%), Postives = 117/204 (57.35%), Query Frame = 1


Query: 1   IHWPFRMKHGSIGFSPENLVAPDIPSTWRAMEALFDSGKARAIGVSNFTINKLRDLLEIA 60
           IHWP RMK  S+GF PENL+ PDIP TWR MEALFDSGKARAIGVSNF+  KL DLL++A
Sbjct: 113 IHWPVRMKKESVGFHPENLLVPDIPGTWREMEALFDSGKARAIGVSNFSSKKLGDLLDVA 172

Query: 61  RIRPAVNE------------------------AYFPLGSRGSTWFKNDDILQNPILKEIA 120
           RI PAVN+                         Y PLGS G+TW K+ D+LQNPILK +A
Sbjct: 173 RIPPAVNQVECHPSWQQTKLHAFCKSKGVHLSGYSPLGSPGTTWIKS-DVLQNPILKMVA 232

Query: 121 EKIGKTPAQVALRWGLQKGHS-----------------------------------ARIV 145
           EK+GKTPAQVALRWGLQ GHS                                   AR+V
Sbjct: 233 EKLGKTPAQVALRWGLQMGHSVLPKSTNESRIKENFDVFGWHIPEDLFAKFHDIEQARLV 292

[Close]

BLAST of Lsi01G014540 vs. TrEMBL
Match: A0A061EQT0_THECC (NAD(P)-linked oxidoreductase superfamily protein OS=Theobroma cacao GN=TCM_021862 PE=4 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 9.1e-44
Identity = 100/204 (49.02%), Postives = 119/204 (58.33%), Query Frame = 1


Query: 1   IHWPFRMKHGSIGFSPENLVAPDIPSTWRAMEALFDSGKARAIGVSNFTINKLRDLLEIA 60
           IHWP RMK GS+GF PENL+ PDIPSTWRAMEAL+DSGKARA+GVSNF+  KL+DLLE+A
Sbjct: 113 IHWPIRMKKGSVGFKPENLLQPDIPSTWRAMEALYDSGKARALGVSNFSSKKLQDLLEVA 172

Query: 61  RIRPAVNEA------------------------YFPLGSRGSTWFKNDDILQNPILKEIA 120
           R+ PAVN+                         Y PLGS G+TW K++ +L+ PIL  +A
Sbjct: 173 RVPPAVNQVECHPSWQQTKLRELCKSKGIHLSGYSPLGSPGTTWLKSE-VLKLPILNMVA 232

Query: 121 EKIGKTPAQVALRWGLQKGHS-----------------------------------ARIV 145
           +K+GKTPAQVALRWGLQ GHS                                   AR+V
Sbjct: 233 QKLGKTPAQVALRWGLQMGHSVLPKSTHEERIKENFDVFDWSIPEDLFAKFSEIEQARLV 292

[Close]

BLAST of Lsi01G014540 vs. TAIR10
Match: AT2G37770.2 (AT2G37770.2 NAD(P)-linked oxidoreductase superfamily protein)

HSP 1 Score: 180.6 bits (457), Expect = 6.7e-46
Identity = 98/204 (48.04%), Postives = 118/204 (57.84%), Query Frame = 1


Query: 1   IHWPFRMKHGSIGFSPENLVAPDIPSTWRAMEALFDSGKARAIGVSNFTINKLRDLLEIA 60
           IHWP R+K GS+G  PENL+  DIPSTW+AMEAL+DSGKARAIGVSNF+  KL DLLE+A
Sbjct: 113 IHWPARIKKGSVGIKPENLLPVDIPSTWKAMEALYDSGKARAIGVSNFSTKKLADLLELA 172

Query: 61  RIRPAVNE------------------------AYFPLGSRGSTWFKNDDILQNPILKEIA 120
           R+ PAVN+                        AY PLGS G+TW K+D +L+NPIL  +A
Sbjct: 173 RVPPAVNQVECHPSWRQTKLQEFCKSKGVHLSAYSPLGSPGTTWLKSD-VLKNPILNMVA 232

Query: 121 EKIGKTPAQVALRWGLQKGHS-----------------------------------ARIV 145
           EK+GK+PAQVALRWGLQ GHS                                   AR+V
Sbjct: 233 EKLGKSPAQVALRWGLQMGHSVLPKSTNEGRIKENFNVFDWSIPDYMFAKFAEIEQARLV 292

[Close]

BLAST of Lsi01G014540 vs. TAIR10
Match: AT2G37790.1 (AT2G37790.1 NAD(P)-linked oxidoreductase superfamily protein)

HSP 1 Score: 173.7 bits (439), Expect = 8.2e-44
Identity = 94/203 (46.31%), Postives = 112/203 (55.17%), Query Frame = 1


Query: 1   IHWPFRMKHGSIGFSPENLVAPDIPSTWRAMEALFDSGKARAIGVSNFTINKLRDLLEIA 60
           IHWP  +K GS GF PEN++  DIPSTW+AME+LFDSGKARAIGVSNF+  KL DLL +A
Sbjct: 113 IHWPVSLKKGSTGFKPENILPTDIPSTWKAMESLFDSGKARAIGVSNFSSKKLADLLVVA 172

Query: 61  RIRPAVNEA------------------------YFPLGSRGSTWFKNDDILQNPILKEIA 120
           R+ PAVN+                         Y PLGS G+TW  +D +L+NPIL  +A
Sbjct: 173 RVPPAVNQVECHPSWQQNVLRDFCKSKGVHLSGYSPLGSPGTTWLTSD-VLKNPILGGVA 232

Query: 121 EKIGKTPAQVALRWGLQKGHS-----------------------------------ARIV 145
           EK+GKTPAQVALRWGLQ G S                                    R+V
Sbjct: 233 EKLGKTPAQVALRWGLQMGQSVLPKSTHEDRIKQNFDVFNWSIPEDMLSKFSEIGQGRLV 292

[Close]

BLAST of Lsi01G014540 vs. TAIR10
Match: AT3G53880.1 (AT3G53880.1 NAD(P)-linked oxidoreductase superfamily protein)

HSP 1 Score: 151.8 bits (382), Expect = 3.3e-37
Identity = 86/204 (42.16%), Postives = 111/204 (54.41%), Query Frame = 1


Query: 1   IHWPFRMKHGSIGFSPENLVAPDIPSTWRAMEALFDSGKARAIGVSNFTINKLRDLLEIA 60
           +HWP R+K G++ F PEN++  DIPSTW+AMEAL DSGKARAIGVSNF+  KL DL+E A
Sbjct: 113 MHWPVRLKKGTVDFKPENIMPIDIPSTWKAMEALVDSGKARAIGVSNFSTKKLSDLVEAA 172

Query: 61  RIRPAVNEA-YFPLGSR-----------------------GSTWFKNDDILQNPILKEIA 120
           R+ PAVN+    P   +                       G+TW K  D+L++P+++ IA
Sbjct: 173 RVPPAVNQVECHPSWQQHKLHEFCKSKGIHLSGYSPLGSPGTTWVK-ADVLKSPVIEMIA 232

Query: 121 EKIGKTPAQVALRWGLQKGHS-----------------------------------ARIV 145
           ++IGK+PAQ ALRWGLQ GHS                                   AR+V
Sbjct: 233 KEIGKSPAQTALRWGLQMGHSILPKSTNEGRIRENFDVLGWSIPKEMFDKFSKIEQARLV 292

[Close]

BLAST of Lsi01G014540 vs. TAIR10
Match: AT2G37760.2 (AT2G37760.2 NAD(P)-linked oxidoreductase superfamily protein)

HSP 1 Score: 148.3 bits (373), Expect = 3.7e-36
Identity = 90/206 (43.69%), Postives = 110/206 (53.40%), Query Frame = 1


Query: 1   IHWPFRMKHGSIGFSPENLVAPDIPSTWRAMEALFDSGKARAIGVSNFTINKLRDLLEIA 60
           IHWP  +K  S+  +PE L  PDI STW+AMEAL+DSGKARAIGVSNF+  KL DLL +A
Sbjct: 109 IHWPASLKKESLMPTPEMLTKPDITSTWKAMEALYDSGKARAIGVSNFSSKKLTDLLNVA 168

Query: 61  RIRPAVNEA------------------------YFPLGS--RGSTWFKNDDILQNPILKE 120
           R+ PAVN+                         Y PLGS  +G    K   +LQNPI+ E
Sbjct: 169 RVTPAVNQVECHPVWQQQGLHELCKSKGVHLSGYSPLGSQSKGEVRLK---VLQNPIVTE 228

Query: 121 IAEKIGKTPAQVALRWGLQKGHS--------ARI----------VLGAMFT--------- 145
           +AEK+GKT AQVALRWGLQ GHS        AR+          +   +FT         
Sbjct: 229 VAEKLGKTTAQVALRWGLQTGHSVLPKSSSGARLKENLDVFDWSIPEDLFTKFSNIPQEK 288

[Close]

BLAST of Lsi01G014540 vs. TAIR10
Match: AT1G59950.1 (AT1G59950.1 NAD(P)-linked oxidoreductase superfamily protein)

HSP 1 Score: 101.7 bits (252), Expect = 3.9e-22
Identity = 68/206 (33.01%), Postives = 93/206 (45.15%), Query Frame = 1


Query: 1   IHWPFRMKHGSIGFSPE--NLVAPDIPSTWRAMEALFDSGKARAIGVSNFTINKLRDLLE 60
           IHWP   K G   F  E  + +  D  + W  ME     G A+ IGVSNF+  KL+ +L 
Sbjct: 116 IHWPVSSKPGKYKFPIEEDDFLPMDYETVWSEMEECQRLGVAKCIGVSNFSCKKLQHILS 175

Query: 61  IARIRPAVNE------------------------AYFPLGSRGSTWFKNDDILQNPILKE 120
           IA+I P+VN+                        AY  LGSRG+ W  +  I+++ +LKE
Sbjct: 176 IAKIPPSVNQVEMSPVWQQRKLRELCKSKGIVVTAYSVLGSRGAFWGTHK-IMESDVLKE 235

Query: 121 IAEKIGKTPAQVALRWGLQKGHS------------------------------------A 145
           IAE  GKT AQV++RW  ++G S                                    +
Sbjct: 236 IAEAKGKTVAQVSMRWAYEEGVSMVVKSFRKDRLEENLKIFDWSLTEEEKQRISTEISQS 295

[Close]

BLAST of Lsi01G014540 vs. NCBI nr
Match: gi|659069123|ref|XP_008448265.1| (PREDICTED: LOW QUALITY PROTEIN: aldo-keto reductase family 4 member C9-like [Cucumis melo])

HSP 1 Score: 217.2 bits (552), Expect = 1.8e-53
Identity = 111/203 (54.68%), Postives = 127/203 (62.56%), Query Frame = 1


Query: 1   IHWPFRMKHGSIGFSPENLVAPDIPSTWRAMEALFDSGKARAIGVSNFTINKLRDLLEIA 60
           IHWP RMKHGS GF+PEN +APDIPSTW+AMEALFDSGKARAIGVSNF+  KL DLLE+A
Sbjct: 113 IHWPVRMKHGSTGFAPENFLAPDIPSTWKAMEALFDSGKARAIGVSNFSSKKLGDLLEVA 172

Query: 61  RIRPAVNEA------------------------YFPLGSRGSTWFKNDDILQNPILKEIA 120
           R+ PAVN+                         Y PLGSRG+TW K  DIL+NPILKEIA
Sbjct: 173 RVPPAVNQVECHPCWQQDKLREFCKSKGVHLSGYSPLGSRGTTWLKGGDILENPILKEIA 232

Query: 121 EKIGKTPAQVALRWGLQKGHS-----------------------------------ARIV 145
           EK+GKTPAQVALRWGLQKGHS                                   AR++
Sbjct: 233 EKLGKTPAQVALRWGLQKGHSVLPKSTSESRIKENLDVFDWSIPEDLFAKFSEFHQARLI 292

[Close]

BLAST of Lsi01G014540 vs. NCBI nr
Match: gi|778693526|ref|XP_011653652.1| (PREDICTED: aldo-keto reductase family 4 member C9 [Cucumis sativus])

HSP 1 Score: 216.5 bits (550), Expect = 3.1e-53
Identity = 111/203 (54.68%), Postives = 127/203 (62.56%), Query Frame = 1


Query: 1   IHWPFRMKHGSIGFSPENLVAPDIPSTWRAMEALFDSGKARAIGVSNFTINKLRDLLEIA 60
           IHWP RMKHGS GF+PEN +APDIPSTWRAMEALFDSGKARAIGVSNF+  KL DLLE+A
Sbjct: 113 IHWPVRMKHGSTGFAPENFLAPDIPSTWRAMEALFDSGKARAIGVSNFSSKKLGDLLEVA 172

Query: 61  RIRPAVNEA------------------------YFPLGSRGSTWFKNDDILQNPILKEIA 120
           R+ PAVN+                         Y PLGSRG+TW K  DIL+NPILKEIA
Sbjct: 173 RVPPAVNQVECHLSWQQDKLREFCKSKGVHLTGYSPLGSRGTTWLKGGDILENPILKEIA 232

Query: 121 EKIGKTPAQVALRWGLQKGHS-----------------------------------ARIV 145
           EK+GKTPAQVALRWGLQKGHS                                   A+++
Sbjct: 233 EKLGKTPAQVALRWGLQKGHSVLPKSTSDSRIKENFDVFDWSIPEDLFAKFSEFHQAKLL 292

[Close]

BLAST of Lsi01G014540 vs. NCBI nr
Match: gi|659069125|ref|XP_008448277.1| (PREDICTED: aldo-keto reductase family 4 member C9-like [Cucumis melo])

HSP 1 Score: 211.5 bits (537), Expect = 1.0e-51
Identity = 108/203 (53.20%), Postives = 124/203 (61.08%), Query Frame = 1


Query: 1   IHWPFRMKHGSIGFSPENLVAPDIPSTWRAMEALFDSGKARAIGVSNFTINKLRDLLEIA 60
           IHWP R+KHGS G +PEN +APDIPSTW+AME LFDSGKARAIGVSN +  KL DLLE+A
Sbjct: 115 IHWPIRLKHGSTGLAPENFLAPDIPSTWKAMEELFDSGKARAIGVSNLSTKKLEDLLEVA 174

Query: 61  RIRPAVNEA------------------------YFPLGSRGSTWFKNDDILQNPILKEIA 120
           R+ PAVN+                         Y PLGSRGSTW K  DIL+NPILKEIA
Sbjct: 175 RVPPAVNQVECHPGWQQNKLREFCKSKGIHLSGYSPLGSRGSTWLKGGDILENPILKEIA 234

Query: 121 EKIGKTPAQVALRWGLQKGHS-----------------------------------ARIV 145
           E++GKTPAQVALRWGLQKGHS                                   AR++
Sbjct: 235 EELGKTPAQVALRWGLQKGHSVLPKSTSESRIKENFDIFDWSIPEDLFAKFSEIEQARVL 294

[Close]

BLAST of Lsi01G014540 vs. NCBI nr
Match: gi|778693522|ref|XP_004142054.2| (PREDICTED: aldo-keto reductase family 4 member C9 [Cucumis sativus])

HSP 1 Score: 208.0 bits (528), Expect = 1.1e-50
Identity = 107/203 (52.71%), Postives = 123/203 (60.59%), Query Frame = 1


Query: 1   IHWPFRMKHGSIGFSPENLVAPDIPSTWRAMEALFDSGKARAIGVSNFTINKLRDLLEIA 60
           IHWP R+KHGS   +PEN VAPDIPSTW+AME L+DSGKARAIGVSN +  KL DLLE+A
Sbjct: 115 IHWPVRLKHGSTDLAPENFVAPDIPSTWKAMEELYDSGKARAIGVSNLSTKKLEDLLEVA 174

Query: 61  RIRPAVNEA------------------------YFPLGSRGSTWFKNDDILQNPILKEIA 120
           RI PAVN+                         Y PLGSRG+TW K  DIL+NPILKEIA
Sbjct: 175 RITPAVNQVECHPGWQQNKLHEFCESKGIHLSGYSPLGSRGTTWLKGGDILENPILKEIA 234

Query: 121 EKIGKTPAQVALRWGLQKGHS-----------------------------------ARIV 145
           E++GKTPAQVALRWGLQKGHS                                   AR++
Sbjct: 235 EELGKTPAQVALRWGLQKGHSILPKSTSESRIKENFDIFDWSIPEDMFAKFSEFEQARVL 294

[Close]

BLAST of Lsi01G014540 vs. NCBI nr
Match: gi|778693529|ref|XP_011653653.1| (PREDICTED: aldo-keto reductase family 4 member C10 [Cucumis sativus])

HSP 1 Score: 205.3 bits (521), Expect = 7.2e-50
Identity = 109/204 (53.43%), Postives = 125/204 (61.27%), Query Frame = 1


Query: 1   IHWPFRMKHGSIGFSPENLVAPDIPSTWRAMEALFDSGKARAIGVSNFTINKLRDLLEIA 60
           +HWP RMKHGS GF+PEN V PDIPSTWRAMEALFDSGKARAIGVSNFTI KL DLLE+A
Sbjct: 113 MHWPVRMKHGSTGFAPENFVTPDIPSTWRAMEALFDSGKARAIGVSNFTIKKLGDLLEVA 172

Query: 61  RIRPAVNEA------------------------YFPLGSRGSTW-FKNDDILQNPILKEI 120
           R+ P+VN+                         Y PLGS G+T  FK  DILQNPILK+I
Sbjct: 173 RVPPSVNQVECHPSWQQDKLREYCKSKGVHLSGYSPLGSFGTTRPFKGGDILQNPILKDI 232

Query: 121 AEKIGKTPAQVALRWGLQKGHSA-----------------------------------RI 145
            +K+GKTPAQVALRWGLQKGHS                                    ++
Sbjct: 233 GDKLGKTPAQVALRWGLQKGHSVLPKSTSELRIKENFDIFNWSIPEDLFAKISEFHQEKV 292

The following BLAST results are available for this feature:

BLAST of Lsi01G014540 vs. Swiss-Prot


Match Name	E-value	Identity	Description
AKRC9_ARATH	1.2e-44	48.04	Aldo-keto reductase family 4 member C9 OS=Arabidopsis thaliana GN=AKR4C9 PE=1 SV...	[more]
AKRCA_ARATH	1.4e-42	46.31	Aldo-keto reductase family 4 member C10 OS=Arabidopsis thaliana GN=AKR4C10 PE=1 ...	[more]
AKRCB_ARATH	5.9e-36	42.16	Aldo-keto reductase family 4 member C11 OS=Arabidopsis thaliana GN=AKR4C11 PE=1 ...	[more]
AKRC8_ARATH	6.5e-35	43.69	Aldo-keto reductase family 4 member C8 OS=Arabidopsis thaliana GN=AKR4C8 PE=1 SV...	[more]
GLD2_HYPJE	6.5e-19	36.91	Glycerol 2-dehydrogenase (NADP(+)) OS=Hypocrea jecorina GN=gld2 PE=1 SV=1	[more]

BLAST of Lsi01G014540 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L2B3_CUCSA	3.6e-48	68.09	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G296830 PE=4 SV=1	[more]
W9QFW8_9ROSA	4.4e-46	55.03	Aldo-keto reductase family 4 member C9 OS=Morus notabilis GN=L484_016975 PE=4 SV...	[more]
A0A0A0L110_CUCSA	8.3e-45	63.83	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G296840 PE=4 SV=1	[more]
W9QNA5_9ROSA	4.1e-44	50.00	Aldo-keto reductase family 4 member C9 OS=Morus notabilis GN=L484_016978 PE=4 SV...	[more]
A0A061EQT0_THECC	9.1e-44	49.02	NAD(P)-linked oxidoreductase superfamily protein OS=Theobroma cacao GN=TCM_02186...	[more]

BLAST of Lsi01G014540 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G37770.2	6.7e-46	48.04	NAD(P)-linked oxidoreductase superfamily protein	[more]
AT2G37790.1	8.2e-44	46.31	NAD(P)-linked oxidoreductase superfamily protein	[more]
AT3G53880.1	3.3e-37	42.16	NAD(P)-linked oxidoreductase superfamily protein	[more]
AT2G37760.2	3.7e-36	43.69	NAD(P)-linked oxidoreductase superfamily protein	[more]
AT1G59950.1	3.9e-22	33.01	NAD(P)-linked oxidoreductase superfamily protein	[more]

BLAST of Lsi01G014540 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659069123\|ref\|XP_008448265.1\|	1.8e-53	54.68	PREDICTED: LOW QUALITY PROTEIN: aldo-keto reductase family 4 member C9-like [Cuc...	[more]
gi\|778693526\|ref\|XP_011653652.1\|	3.1e-53	54.68	PREDICTED: aldo-keto reductase family 4 member C9 [Cucumis sativus]	[more]
gi\|659069125\|ref\|XP_008448277.1\|	1.0e-51	53.20	PREDICTED: aldo-keto reductase family 4 member C9-like [Cucumis melo]	[more]
gi\|778693522\|ref\|XP_004142054.2\|	1.1e-50	52.71	PREDICTED: aldo-keto reductase family 4 member C9 [Cucumis sativus]	[more]
gi\|778693529\|ref\|XP_011653653.1\|	7.2e-50	53.43	PREDICTED: aldo-keto reductase family 4 member C10 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR023210	NADP_OxRdtase_dom
IPR018170	Aldo/ket_reductase_CS
IPR001395	Aldo/keto reductase/potassium channel subunit beta

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0006000	fructose metabolic process
biological_process	GO:0006012	galactose metabolic process
biological_process	GO:0046486	glycerolipid metabolic process
biological_process	GO:0006013	mannose metabolic process
biological_process	GO:0006090	pyruvate metabolic process
biological_process	GO:0019384	caprolactam catabolic process
biological_process	GO:0006094	gluconeogenesis
biological_process	GO:0006096	glycolytic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0004032	alditol:NADP+ 1-oxidoreductase activity
molecular_function	GO:0008106	alcohol dehydrogenase (NADP+) activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G014540.1	Lsi01G014540.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001395	Aldo/keto reductase/potassium channel subunit beta	PANTHER	PTHR11732	ALDO/KETO REDUCTASE	coord: 19..141 score: 2.9
IPR018170	Aldo/keto reductase, conserved site	PROSITE	PS00062	ALDOKETO_REDUCTASE_2	coord: 31..48 scor
IPR023210	NADP-dependent oxidoreductase domain	GENE3D	G3DSA:3.20.20.100		coord: 1..68 score: 3.5E-17coord: 69..117 score: 1.6
IPR023210	NADP-dependent oxidoreductase domain	PFAM	PF00248	Aldo_ket_red	coord: 1..71 score: 5.
IPR023210	NADP-dependent oxidoreductase domain	unknown	SSF51430	NAD(P)-linked oxidoreductase	coord: 1..124 score: 1.19
None	No IPR available	PANTHER	PTHR11732:SF223	ALDO-KETO REDUCTASE FAMILY 4 MEMBER C10	coord: 19..141 score: 2.9

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi01G014540	Csa4G296810	Cucumber (Chinese Long) v2	culsiB248
Lsi01G014540	Cucsa.066450	Cucumber (Gy14) v1	cgylsiB059
Lsi01G014540	Cla018184	Watermelon (97103) v1	lsiwmB152
Lsi01G014540	ClCG04G007890	Watermelon (Charleston Gray)	lsiwcgB142
Lsi01G014540	MELO3C003155.2	Melon (DHL92) v3.6.1	lsimedB158
Lsi01G014540	Cla97C04G074540	Watermelon (97103) v2	lsiwmbB140
Lsi01G014540	Bhi03G001591	Wax gourd	lsiwgoB169
Lsi01G014540	Cp4.1LG10g08480	Cucurbita pepo (Zucchini)	cpelsiB048
Lsi01G014540	Cp4.1LG19g03340	Cucurbita pepo (Zucchini)	cpelsiB396
Lsi01G014540	Carg13232	Silver-seed gourd	carlsiB526
Lsi01G014540	Carg19950	Silver-seed gourd	carlsiB503
Lsi01G014540	CsaV3_4G025300	Cucumber (Chinese Long) v3	cuclsiB272

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi01G014540	Wild cucumber (PI 183967)	cpilsiB247
Lsi01G014540	Wild cucumber (PI 183967)	cpilsiB255
Lsi01G014540	Wild cucumber (PI 183967)	cpilsiB345
Lsi01G014540	Cucumber (Chinese Long) v2	culsiB341
Lsi01G014540	Melon (DHL92) v3.5.1	lsimeB151
Lsi01G014540	Watermelon (Charleston Gray)	lsiwcgB148
Lsi01G014540	Cucumber (Gy14) v2	cgyblsiB236
Lsi01G014540	Melon (DHL92) v3.6.1	lsimedB129
Lsi01G014540	Cucumber (Chinese Long) v3	cuclsiB365
Lsi01G014540	Watermelon (97103) v2	lsiwmbB146
Lsi01G014540	Bottle gourd (USVL1VR-Ls)	lsilsiB077
Lsi01G014540	Cucurbita maxima (Rimu)	cmalsiB601
Lsi01G014540	Cucurbita maxima (Rimu)	cmalsiB642
Lsi01G014540	Cucurbita maxima (Rimu)	cmalsiB644
Lsi01G014540	Cucurbita maxima (Rimu)	cmalsiB786
Lsi01G014540	Cucurbita moschata (Rifu)	cmolsiB587
Lsi01G014540	Cucurbita moschata (Rifu)	cmolsiB630
Lsi01G014540	Cucurbita moschata (Rifu)	cmolsiB631
Lsi01G014540	Cucurbita moschata (Rifu)	cmolsiB774