Lsi01G011650 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi01G011650
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Cysteine proteinase inhibitor 5
Location	chr01 : 9802787 .. 9804337 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAACTAATTGTTAATTAAGTTTAAAGAAGGAATGGAATAAATTGGAGTTGTATAAATTTGAAAGGAAGCACACAAAGCAAAAGCATTCCATAAGTAATAACTTTGTGGGAAAAAGTTTGTGTATTGAAAGTAAGTTAAGAAAATGTGTTCTCAGTTGCTCGGCAGTTATAGTCCATGTGAAGATCCCGAAGGCCCGCACGTAAAAGAAGTAGCAGAATGGGCAGTAGCAGAGTACAACAAAGGCGGACATTGCTTGACATTGATTCATGTGTTAAGTTGTGAGTCGCAGGTGGTGGCCGGAGTCAACTACCGCCTTGTCTTGTTAGTTAAGGATGAAAAAAATCATGAGAAAGTCTACGAGACTGTTGTGTGGGAGAAGATATGGGAGAATTTTAGGAAGCTCATTTACTTTAAACCTTACAAGGTATGCTTTTTCTTATAGCACTTACATGCTTTCTTTTGCAACCCTTTTCAAATTTGTTATTTGTATCTATATTTGGTTACTTAATGAAGTGTTCTCTAACTGATGAAACAAAATAAAAATCATAAATTGAGTTTGGTAAAGTTGAGTCTATTTTATTCAAATTAAAAATAGGATGATATATATTAGTTTGTACTCGGATAGTAGAAGCATGCATGACATATATAGTTGAATTTTGGGATTCAAAATTTCTTTTGTCTTAATAAAGCTATTTTTCAGTCTTAATGATCATTTAAGAAATTAGTACGCAAAAAGTGGAATAAAAATTGTAGTAGTACAAATTTGAAAATCATTCCATATTTGGTTTAACTTTTCAAATGTTTACTTTTGAAATAAGTTATTTTAAGAATAATTCAAATATTTGGTAACGGCTTAAAATAGCTTTTGAAATACTCTCAAACTATGTTTCAAACATTTTTTTTTAATCAAAAAAGAGTTTAAATAATAATGATTTTTTTTAAAAAAAATATTTTTTCTAGACAATCCAAATGTCTAAGAGGCTGTTTGGGGGACAGTAATTGGATGGGTTTAGGTTGTAACGTAATTTAAAAATCTACGTTTGAATTAAACATTTTAGGCTCGATTTGTAATACTAAACTCATTCCGTTCTCACAGTTCTCAACCTAACTCTCTTTCCCACCGTTTTCATGCTTCACGATCTCATTTTCGTTATGTCATAAATTCCTCACACATTCCAATACCCCTCAGATTTCTAATAATTTGATAACATTTCAGCCCCAAACAATCCCTAATTGTGTATATTGAAAGTAAGGAAAATGCGTTCTCAGGTAGTTGGCGGTTATGGTCCATGTAAGGATCCTGAAGGCCCACATGTGAAAGAAATAGCAGAATGGGCAGTAGCAGAGTACAACAAAGGCGGACATTGCTTGACATTGATTAGTGTGTTGAAATGCGAGTCGCAGGTGGTGGCCGGAGTCAACTACCGCCTTGTCTTGTTGGCTAAGGATGATAAAAATCATGAGGGAAAATATGAGGCTGTTGTGTGGGAGAAGATATGGGAGCATTTCAGGAAGCTCATTAGCTTTAAACCTCTCTTGTTATGTTGA

mRNA sequence

AAAAAACTAATTGTTAATTAAGTTTAAAGAAGGAATGGAATAAATTGGAGTTGTATAAATTTGAAAGGAAGCACACAAAGCAAAAGCATTCCATAAGTAATAACTTTGTGGGAAAAAGTTTGTGTATTGAAAGTAAGTTAAGAAAATGTGTTCTCAGTTGCTCGGCAGTTATAGTCCATGTGAAGATCCCGAAGGCCCGCACGTAAAAGAAGTAGCAGAATGGGCAGTAGCAGAGTACAACAAAGGCGGACATTGCTTGACATTGATTCATGTGTTAAGTTGTGAGTCGCAGGTGGTGGCCGGAGTCAACTACCGCCTTGTCTTGTTAGTTAAGGATGAAAAAAATCATGAGAAAGTCTACGAGACTGTTGTGTGGGAGAAGATATGGGAGAATTTTAGGAAGCTCATTTACTTTAAACCTTACAAGGATCCTGAAGGCCCACATGTGAAAGAAATAGCAGAATGGGCAGTAGCAGAGTACAACAAAGGCGGACATTGCTTGACATTGATTAGTGTGTTGAAATGCGAGTCGCAGGTGGTGGCCGGAGTCAACTACCGCCTTGTCTTGTTGGCTAAGGATGATAAAAATCATGAGGGAAAATATGAGGCTGTTGTGTGGGAGAAGATATGGGAGCATTTCAGGAAGCTCATTAGCTTTAAACCTCTCTTGTTATGTTGA

Coding sequence (CDS)

ATGTGTTCTCAGTTGCTCGGCAGTTATAGTCCATGTGAAGATCCCGAAGGCCCGCACGTAAAAGAAGTAGCAGAATGGGCAGTAGCAGAGTACAACAAAGGCGGACATTGCTTGACATTGATTCATGTGTTAAGTTGTGAGTCGCAGGTGGTGGCCGGAGTCAACTACCGCCTTGTCTTGTTAGTTAAGGATGAAAAAAATCATGAGAAAGTCTACGAGACTGTTGTGTGGGAGAAGATATGGGAGAATTTTAGGAAGCTCATTTACTTTAAACCTTACAAGGATCCTGAAGGCCCACATGTGAAAGAAATAGCAGAATGGGCAGTAGCAGAGTACAACAAAGGCGGACATTGCTTGACATTGATTAGTGTGTTGAAATGCGAGTCGCAGGTGGTGGCCGGAGTCAACTACCGCCTTGTCTTGTTGGCTAAGGATGATAAAAATCATGAGGGAAAATATGAGGCTGTTGTGTGGGAGAAGATATGGGAGCATTTCAGGAAGCTCATTAGCTTTAAACCTCTCTTGTTATGTTGA

Protein sequence

MCSQLLGSYSPCEDPEGPHVKEVAEWAVAEYNKGGHCLTLIHVLSCESQVVAGVNYRLVLLVKDEKNHEKVYETVVWEKIWENFRKLIYFKPYKDPEGPHVKEIAEWAVAEYNKGGHCLTLISVLKCESQVVAGVNYRLVLLAKDDKNHEGKYEAVVWEKIWEHFRKLISFKPLLLC

Homology

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BLAST of Lsi01G011650 vs. Swiss-Prot
Match: CYTM_SOLTU (Multicystatin OS=Solanum tuberosum PE=1 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 9.2e-23
Identity = 62/182 (34.07%), Postives = 99/182 (54.40%), Query Frame = 1


Query: 6   LGSYSPCEDPEGPHVKEVAEWAVAEYNKGGHC-LTLIHVLSCESQVVAGVNYRLVLLVKD 65
           LG ++    P  P  +++  +AV +YNK  +  L  +  L+ + QVVAG+ Y +     D
Sbjct: 382 LGGFTEVPFPNSPEFQDLTRFAVHQYNKDQNAHLEFVENLNVKKQVVAGMLYYITFAATD 441

Query: 66  EKNHEKVYETVVWEKIWENFRKLIYFKPYKD-------------PEGPHVKEIAEWAVAE 125
               +K+YET +W K+WENF+K++ FK   D             P  P  +++A +AV +
Sbjct: 442 G-GKKKIYETKIWVKVWENFKKVVEFKLVGDDSAKLGGIINVPFPNNPEFQDLARFAVQD 501

Query: 126 YNKGGHC-LTLISVLKCESQVVAGVNYRLVLLAKDDKNHEGKYEAVVWEKIWEHFRKLIS 173
           YNK  +  L  +  L  + Q+VAG+ Y + L+A  D   +  YEA +W K WE+F+K+I 
Sbjct: 502 YNKKENAHLEFVENLNVKEQLVAGMLYYITLVA-IDAGKKKIYEAKIWVKEWENFKKVIE 561

[Close]

BLAST of Lsi01G011650 vs. Swiss-Prot
Match: CYT1_ACTDE (Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa PE=1 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 3.3e-12
Identity = 42/92 (45.65%), Postives = 60/92 (65.22%), Query Frame = 1


Query: 85  RKLIY---FKPYKDPEGPHVKEIAEWAVAEYNKGGHC-LTLISVLKCESQVVAGVNYRLV 144
           RKL+    ++P +      V+++A++AV+E+NK  +  L   SV++  +QVVAG NYRLV
Sbjct: 24  RKLVAAGGWRPIESLNSAEVQDVAQFAVSEHNKQANDELQYQSVVRGYTQVVAGTNYRLV 83

Query: 145 LLAKDDKNHEGKYEAVVWEKIWEHFRKLISFK 173
           + AKD     G YEAVVW+K W HFR L SF+
Sbjct: 84  IAAKDGAV-VGNYEAVVWDKPWMHFRNLTSFR 114

[Close]

BLAST of Lsi01G011650 vs. Swiss-Prot
Match: CYT5_ARATH (Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana GN=CYS5 PE=2 SV=2)

HSP 1 Score: 70.1 bits (170), Expect = 2.8e-11
Identity = 37/85 (43.53%), Postives = 49/85 (57.65%), Query Frame = 1


Query: 90  FKPYKDPEGPHVKEIAEWAVAEYNKGGHC-LTLISVLKCESQVVAGVNYRLVLLAKDDKN 149
           + P  +   P V EI E+AV+EYNK     L   +V+  E+QVV+G NYRL + A D   
Sbjct: 31  WSPISNVTDPQVVEIGEFAVSEYNKRSESGLKFETVVSGETQVVSGTNYRLKVAANDGDG 90

Query: 150 HEGKYEAVVWEKIWEHFRKLISFKP 174
               Y A+VW+K W  FR L SF+P
Sbjct: 91  VSKNYLAIVWDKPWMKFRNLTSFEP 115

[Close]

BLAST of Lsi01G011650 vs. Swiss-Prot
Match: CYT7_ORYSJ (Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica GN=Os03g0210100 PE=3 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 4.7e-11
Identity = 40/83 (48.19%), Postives = 52/83 (62.65%), Query Frame = 1


Query: 95  DPEGPHVKEIAEWAVAEYNKGGHC--LTLISVLKCESQVVAGVNYRLVLLAKDDKNHEGK 154
           D   PHV+E+  WAVAE ++G     LT   V   E QVVAGVNYRL L A      +G+
Sbjct: 34  DINDPHVQELGRWAVAEEDRGVAAGGLTFERVTDGEKQVVAGVNYRLTLEASSSGAKDGR 93

Query: 155 YEAVVWEK-IWEHFRKLISFKPL 175
           YEAVV+E+    + RKL+SF+P+
Sbjct: 94  YEAVVYEQDPRSNARKLVSFEPI 116

[Close]

BLAST of Lsi01G011650 vs. Swiss-Prot
Match: CYT8_ORYSJ (Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica GN=Os03g0429000 PE=2 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 4.0e-10
Identity = 36/89 (40.45%), Postives = 49/89 (55.06%), Query Frame = 1


Query: 5   LLGSYSPCEDPEGPHVKEVAEWAVAEY-NKGGHCLTLIHVLSCESQVVAGVNYRLVLLVK 64
           L+G +SP  D   PH++E+  WAV  + +     L    V S E QVV+G+NYRLV+   
Sbjct: 31  LVGGWSPITDVGDPHIQELGGWAVERHASLSSDGLRFRRVTSGEQQVVSGMNYRLVVSAS 90

Query: 65  DEKNHEKVYETVVWEKIWENFRKLIYFKP 93
           D       Y  VV+E+ W N R+L  FKP
Sbjct: 91  DPAGATASYVAVVYEQSWTNTRQLTSFKP 119

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BLAST of Lsi01G011650 vs. TrEMBL
Match: V4T622_9ROSI (Cysteine proteinase inhibitor OS=Citrus clementina GN=CICLE_v10002632mg PE=3 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 4.3e-35
Identity = 88/182 (48.35%), Postives = 117/182 (64.29%), Query Frame = 1


Query: 5   LLGSYSPCEDPEGPHVKEVAEWAVAEYNKGGH-CLTLIHVLSCESQVVAGVNYRLVLLVK 64
           +LG + P EDP+  HV E+ ++AV EYNK     L    V   E+QVV+G NYRL+L+VK
Sbjct: 1   MLGGWKPIEDPKEKHVMEIGQFAVTEYNKRSKSALKFESVEKGETQVVSGTNYRLILVVK 60

Query: 65  DEKNHEKVYETVVWEKIWENFRKLIYFKPY---------KDPEGPHVKEIAEWAVAEYNK 124
           D  + +K +E VV EK WE+F+ L  FKP          +DP+  HV EI ++AV EYNK
Sbjct: 61  DGPSTKK-FEAVVLEKPWEHFKSLTSFKPMPMLGGWKPIEDPKEKHVMEIGQFAVTEYNK 120

Query: 125 GGH-CLTLISVLKCESQVVAGVNYRLVLLAKDDKNHEGKYEAVVWEKIWEHFRKLISFKP 176
                L   SV K E+QVV+G NYRL+L+ KD  + + K+EAVVWEK WEHF+ L SFKP
Sbjct: 121 RSKGALKFESVEKGETQVVSGTNYRLILVVKDGPSTK-KFEAVVWEKPWEHFKSLTSFKP 180

[Close]

BLAST of Lsi01G011650 vs. TrEMBL
Match: O80389_CUCSA (Cysteine proteinase inhibitor OS=Cucumis sativus PE=2 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 1.7e-28
Identity = 64/91 (70.33%), Postives = 72/91 (79.12%), Query Frame = 1


Query: 1  MCSQLLGSYSPCEDPEGPHVKEVAEWAVAEYNKG-GHCLTLIHVLSCESQVVAGVNYRLV 60
          M S  +G Y PC+DP  PHVK++AEWAVAEYNK  GH LTL+ +L CESQVVAGVN+RLV
Sbjct: 1  MSSSEIGGYVPCKDPNDPHVKDIAEWAVAEYNKSQGHHLTLVSILKCESQVVAGVNWRLV 60

Query: 61 LLVKDEKNHEKVYETVVWEKIWENFRKLIYF 91
          L  KDE N E  YETVVWEKIWENFR+LI F
Sbjct: 61 LKCKDENNGEGNYETVVWEKIWENFRQLITF 91

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BLAST of Lsi01G011650 vs. TrEMBL
Match: A0A078EQE2_BRANA (Cysteine proteinase inhibitor OS=Brassica napus GN=BnaA09g19480D PE=3 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.8e-25
Identity = 76/189 (40.21%), Postives = 105/189 (55.56%), Query Frame = 1


Query: 5   LLGSYSPCEDPEGPHVKEVAEWAVAEYNKGGHC-LTLIHVLSCESQVVAGVNYRLVLLVK 64
           L G +SP  D +  H+ E+ E+AV+EYNK     L  + V+S ESQ+VAG+NYRL+L   
Sbjct: 25  LTGGWSPISDVKNAHIVEIGEFAVSEYNKQSKSGLKFVEVVSGESQIVAGMNYRLILAAN 84

Query: 65  D-----EKNHEKVYETVVWEKIWENFRKLIYFKP------YKDPEGPHVKEIAEWAVAEY 124
           +          K YE VVWEK W     L  FKP          +    ++I E+AV+EY
Sbjct: 85  EGVAIAGNGESKKYEAVVWEKPWLKSMNLTSFKPAMLTRKQLRHKALTTQKIGEFAVSEY 144

Query: 125 NKGGHC-LTLISVLKCESQVVAGVNYRLVLLAKDD----KNHEG-KYEAVVWEKIWEHFR 176
           +K     L  + V+  ESQ+VAG+NYRL++ A +     +N E  KYEA+VW+K W    
Sbjct: 145 DKQSKSGLKFVEVVSGESQIVAGMNYRLIVAANEGIPIARNGESKKYEAIVWDKPWLKSM 204

[Close]

BLAST of Lsi01G011650 vs. TrEMBL
Match: M1A5P9_SOLTU (Cysteine proteinase inhibitor OS=Solanum tuberosum GN=PGSC0003DMG400005950 PE=3 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 9.9e-24
Identity = 65/182 (35.71%), Postives = 99/182 (54.40%), Query Frame = 1


Query: 6   LGSYSPCEDPEGPHVKEVAEWAVAEYNKGGHC-LTLIHVLSCESQVVAGVNYRLVLLVKD 65
           LG ++    P  P  +++  +AV +YNK  +  L  +  L+ + QVVAG+ Y +     D
Sbjct: 46  LGGFTEVPFPNSPEFQDLTRFAVHQYNKDQNAHLEFVENLNVKKQVVAGMLYYITFAATD 105

Query: 66  EKNHEKVYETVVWEKIWENFRKLIYFKPYKD-------------PEGPHVKEIAEWAVAE 125
               +K+YET +W K+WENF+K++ FK   D             P  P  +++A +AV +
Sbjct: 106 G-GKKKIYETKIWVKVWENFKKVVEFKLVGDDSAKPGGIINVPFPNSPEFQDLARFAVQD 165

Query: 126 YNKGGHC-LTLISVLKCESQVVAGVNYRLVLLAKDDKNHEGKYEAVVWEKIWEHFRKLIS 173
           YNK     L  +  L  + QVVAG+ Y + L+A DD   E  YEA +W K WE+F+K+I 
Sbjct: 166 YNKKEKAHLEFVENLNVKEQVVAGMMYYITLVATDDAGKEKIYEAKIWVKEWENFKKVIE 225

[Close]

BLAST of Lsi01G011650 vs. TrEMBL
Match: M4DLH7_BRARP (Cysteine proteinase inhibitor OS=Brassica rapa subsp. pekinensis PE=3 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 9.9e-24
Identity = 74/189 (39.15%), Postives = 103/189 (54.50%), Query Frame = 1


Query: 5   LLGSYSPCEDPEGPHVKEVAEWAVAEYNKGGHC-LTLIHVLSCESQVVAGVNYRLVLLVK 64
           L G +SP  D +   + E+ E+AV+EYNK     L  + V+S ESQ+ AG+NYRL+L   
Sbjct: 25  LTGGWSPISDVKNARIVEIGEFAVSEYNKQSKSGLKFVEVVSGESQIAAGMNYRLILADN 84

Query: 65  DE-----KNHEKVYETVVWEKIWENFRKLIYFKPYK------DPEGPHVKEIAEWAVAEY 124
           +          K YE VVWEK W     L  FKP          +    ++I E+AV+EY
Sbjct: 85  EGVAIAGNGESKKYEAVVWEKPWLKSMNLTSFKPAMLTRKQLRHKALTTQKIGEFAVSEY 144

Query: 125 NKGGHC-LTLISVLKCESQVVAGVNYRLVLLAKDD----KNHEGK-YEAVVWEKIWEHFR 176
           +K     L  + V+  ESQ+VAG+NYRL++ A +     +N E K YEA+VW+K W    
Sbjct: 145 DKQSKSGLKFVEVVSGESQIVAGMNYRLIVAANEGIPIARNGESKKYEAIVWDKPWLKSM 204

[Close]

BLAST of Lsi01G011650 vs. TAIR10
Match: AT5G47550.1 (AT5G47550.1 Cystatin/monellin superfamily protein)

HSP 1 Score: 70.1 bits (170), Expect = 1.6e-12
Identity = 37/85 (43.53%), Postives = 49/85 (57.65%), Query Frame = 1


Query: 90  FKPYKDPEGPHVKEIAEWAVAEYNKGGHC-LTLISVLKCESQVVAGVNYRLVLLAKDDKN 149
           + P  +   P V EI E+AV+EYNK     L   +V+  E+QVV+G NYRL + A D   
Sbjct: 31  WSPISNVTDPQVVEIGEFAVSEYNKRSESGLKFETVVSGETQVVSGTNYRLKVAANDGDG 90

Query: 150 HEGKYEAVVWEKIWEHFRKLISFKP 174
               Y A+VW+K W  FR L SF+P
Sbjct: 91  VSKNYLAIVWDKPWMKFRNLTSFEP 115

[Close]

BLAST of Lsi01G011650 vs. TAIR10
Match: AT4G16500.1 (AT4G16500.1 Cystatin/monellin superfamily protein)

HSP 1 Score: 63.5 bits (153), Expect = 1.5e-10
Identity = 35/85 (41.18%), Postives = 49/85 (57.65%), Query Frame = 1


Query: 91  KPYKDPEGPHVKEIAEWAVAEYNKGG-HCLTLISVLKCESQVVAGVNYRLVLLAKDDKNH 150
           KP K+   P V  +A++A+ E+NK     L  + V++  +QVV+G  Y L + AKD    
Sbjct: 33  KPIKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVVSGTKYDLKIAAKDGGGK 92

Query: 151 EGKYEAVVWEKIWEHFRKLISFKPL 175
              YEAVV EK+W H + L SFK L
Sbjct: 93  IKNYEAVVVEKLWLHSKSLESFKAL 117

[Close]

BLAST of Lsi01G011650 vs. TAIR10
Match: AT3G12490.2 (AT3G12490.2 cystatin B)

HSP 1 Score: 57.4 bits (137), Expect = 1.0e-08
Identity = 50/172 (29.07%), Postives = 74/172 (43.02%), Query Frame = 1


Query: 20  VKEVAEWAVAEYNKGGHCLT-LIHVLSCESQVVAGVNYRLVLLVKDEKNHEKVYETVVWE 79
           V+ +A +AV E+NK  + L     V+  + QVVAG  + L L +  E   +K+YE  VW 
Sbjct: 52  VESLARFAVDEHNKKENALLEFARVVKAKEQVVAGTLHHLTLEIL-EAGQKKLYEAKVWV 111

Query: 80  KIWENFRKLIYFKPYKDP------------------------EGPHVKEIAEWAVAEYNK 139
           K W NF++L  FKP  D                         + P VK +AE AV    +
Sbjct: 112 KPWLNFKELQEFKPASDAPAITSSDLGCKQGEHESGWREVPGDDPEVKHVAEQAVKTIQQ 171

Query: 140 GGHCL---TLISVLKCESQVVA-GVNYRLVLLAKD-----------DKNHEG 152
             + L    L+ V+  +++V      Y ++L  K             KNHEG
Sbjct: 172 RSNSLFPYELLEVVHAKAEVTGEAAKYNMLLKLKRGEKEEKFKVEVHKNHEG 222

[Close]

BLAST of Lsi01G011650 vs. TAIR10
Match: AT5G05110.1 (AT5G05110.1 Cystatin/monellin family protein)

HSP 1 Score: 55.1 bits (131), Expect = 5.2e-08
Identity = 36/89 (40.45%), Postives = 50/89 (56.18%), Query Frame = 1


Query: 6   LGSYSPCEDP--EGPHVKEVAEWAVAEYNKGGHC-LTLIHVLSCESQVVAGVNYRLVLLV 65
           LG +S  ++    G  + ++A +AV E+N+  +  L L  VL    QVVAG  YRL L V
Sbjct: 45  LGGFSDSKNDWNGGKEIDDIALFAVQEHNRRENAVLELARVLKATEQVVAGKLYRLTLEV 104

Query: 66  KDEKNHEKVYETVVWEKIWENFRKLIYFK 92
             E   +K+YE  VW K W NF++L  FK
Sbjct: 105 I-EAGEKKIYEAKVWVKPWMNFKQLQEFK 132

[Close]

BLAST of Lsi01G011650 vs. TAIR10
Match: AT2G40880.1 (AT2G40880.1 cystatin A)

HSP 1 Score: 49.3 bits (116), Expect = 2.9e-06
Identity = 27/73 (36.99%), Postives = 42/73 (57.53%), Query Frame = 1


Query: 101 VKEIAEWAVAEYNKGGH-CLTLISVLKCESQVVAGVNYRLVLLAKDDKNHEGKYEAVVWE 160
           ++ +A +A+ E+NK  +  L    ++K   QVVAG  Y L L AK+  +    +EA VW 
Sbjct: 50  IESLARFAIQEHNKQQNKILEFKKIVKAREQVVAGTMYHLTLEAKEG-DQTKNFEAKVWV 109

Query: 161 KIWEHFRKLISFK 173
           K W +F++L  FK
Sbjct: 110 KPWMNFKQLQEFK 121

[Close]

BLAST of Lsi01G011650 vs. NCBI nr
Match: gi|695018835|ref|XP_009396392.1| (PREDICTED: cysteine proteinase inhibitor 1 {ECO:0000303|PubMed:14697268}-like [Musa acuminata subsp. malaccensis])

HSP 1 Score: 156.8 bits (395), Expect = 3.6e-35
Identity = 94/180 (52.22%), Postives = 117/180 (65.00%), Query Frame = 1


Query: 7   GSYSPCEDPEGPHVKEVAEWAVAEYNKGGHC-LTLIHVLSCESQVVAGVNYRLVLLVKDE 66
           G ++  ED + PHV+E+AE+AV+E NK     LTL  V   E+QVVAG NYRLVL V+D 
Sbjct: 33  GGWTAIEDLKDPHVREIAEFAVSENNKLEKTNLTLRKVEGGETQVVAGTNYRLVLEVRDG 92

Query: 67  KNHEKVYETVVWEKIWENFRKLIYF---------KPYKDPEGPHVKEIAEWAVAEYN-KG 126
                 YE VVWEK WE+F++L  F          P K+   P V EIAE+AVAE+N + 
Sbjct: 93  SGASARYEAVVWEKPWESFKQLASFHKLVIVGGWTPIKNISDPDVGEIAEFAVAEHNHEA 152

Query: 127 GHCLTLISVLKCESQVVAGVNYRLVLLAKD-DKNHEGKYEAVVWEKIWEHFRKLISFKPL 175
           G  LTL  V+K E+QVVAG+NY+LV+ AKD       +YEAVVWEK WEHFR+L SFK L
Sbjct: 153 GTNLTLCKVVKGETQVVAGINYKLVIEAKDGGVGVVSEYEAVVWEKTWEHFRRLTSFKLL 212

[Close]

BLAST of Lsi01G011650 vs. NCBI nr
Match: gi|567878425|ref|XP_006431771.1| (hypothetical protein CICLE_v10002632mg [Citrus clementina])

HSP 1 Score: 156.0 bits (393), Expect = 6.1e-35
Identity = 88/182 (48.35%), Postives = 117/182 (64.29%), Query Frame = 1


Query: 5   LLGSYSPCEDPEGPHVKEVAEWAVAEYNKGGH-CLTLIHVLSCESQVVAGVNYRLVLLVK 64
           +LG + P EDP+  HV E+ ++AV EYNK     L    V   E+QVV+G NYRL+L+VK
Sbjct: 1   MLGGWKPIEDPKEKHVMEIGQFAVTEYNKRSKSALKFESVEKGETQVVSGTNYRLILVVK 60

Query: 65  DEKNHEKVYETVVWEKIWENFRKLIYFKPY---------KDPEGPHVKEIAEWAVAEYNK 124
           D  + +K +E VV EK WE+F+ L  FKP          +DP+  HV EI ++AV EYNK
Sbjct: 61  DGPSTKK-FEAVVLEKPWEHFKSLTSFKPMPMLGGWKPIEDPKEKHVMEIGQFAVTEYNK 120

Query: 125 GGH-CLTLISVLKCESQVVAGVNYRLVLLAKDDKNHEGKYEAVVWEKIWEHFRKLISFKP 176
                L   SV K E+QVV+G NYRL+L+ KD  + + K+EAVVWEK WEHF+ L SFKP
Sbjct: 121 RSKGALKFESVEKGETQVVSGTNYRLILVVKDGPSTK-KFEAVVWEKPWEHFKSLTSFKP 180

[Close]

BLAST of Lsi01G011650 vs. NCBI nr
Match: gi|568825922|ref|XP_006467325.1| (PREDICTED: multicystatin [Citrus sinensis])

HSP 1 Score: 152.5 bits (384), Expect = 6.8e-34
Identity = 86/178 (48.31%), Postives = 112/178 (62.92%), Query Frame = 1


Query: 5   LLGSYSPCEDPEGPHVKEVAEWAVAEYNKGGH-CLTLIHVLSCESQVVAGVNYRLVLLVK 64
           L+G + P EDP+  HV E+ ++AV EYNK     L    V   E+QVV+G NYRL+L+VK
Sbjct: 28  LVGGWKPIEDPKEKHVMEIGQFAVTEYNKQSKSALKFESVEKGETQVVSGTNYRLILVVK 87

Query: 65  DEKNHEKVYETVVWEKIWENFRKLIYFKPYK------DPEGPHVKEIAEWAVAEYNKGGH 124
           D  + +K +E VVWEK WE+F+ L  FKP        DP   HV EI ++AV EYNK   
Sbjct: 88  DGPSTKK-FEAVVWEKPWEHFKSLTSFKPMLGGWTPIDPTEKHVMEIGQFAVTEYNKRSK 147

Query: 125 -CLTLISVLKCESQVVAGVNYRLVLLAKDDKNHEGKYEAVVWEKIWEHFRKLISFKPL 175
             L   SV K  +QVV+G NYRL+L+ KD+ + + K   VVWEK WEHF+ L SFKP+
Sbjct: 148 SALKFESVEKAWTQVVSGTNYRLILVVKDEPSTK-KLLVVVWEKPWEHFKSLTSFKPM 203

[Close]

BLAST of Lsi01G011650 vs. NCBI nr
Match: gi|568837934|ref|XP_006472972.1| (PREDICTED: multicystatin-like, partial [Citrus sinensis])

HSP 1 Score: 149.4 bits (376), Expect = 5.7e-33
Identity = 85/176 (48.30%), Postives = 111/176 (63.07%), Query Frame = 1


Query: 7   GSYSPCEDPEGPHVKEVAEWAVAEYNKGGHCLTLIH-VLSCESQVVAGVNYRLVLLVKDE 66
           G ++P  DP   HV E+ ++AV EYNK   C      V +  +QVV+G NYRL+L+VKD 
Sbjct: 3   GGWTPI-DPTEKHVMEIGQFAVTEYNKRSKCAFKFESVENAWTQVVSGTNYRLILIVKDG 62

Query: 67  KNHEKVYETVVWEKIWENFRKLIYFKPYK------DPEGPHVKEIAEWAVAEYNKGGH-C 126
            + +K +E VVWEK WE+F+ L  FKP        DP   HV  I ++AV EYNK     
Sbjct: 63  ASTKK-FEAVVWEKPWEHFKSLTSFKPMPGGWTPIDPTEKHVMVIVQFAVTEYNKRSKSA 122

Query: 127 LTLISVLKCESQVVAGVNYRLVLLAKDDKNHEGKYEAVVWEKIWEHFRKLISFKPL 175
           L   SV K E+QVV+G NYRL+L+ KD  + + K+EAVVWEK WEHF+ L SFKP+
Sbjct: 123 LKFESVEKAETQVVSGTNYRLILVVKDGLSTK-KFEAVVWEKPWEHFKSLTSFKPM 175

[Close]

BLAST of Lsi01G011650 vs. NCBI nr
Match: gi|568834137|ref|XP_006471203.1| (PREDICTED: cysteine proteinase inhibitor 5-like [Citrus sinensis])

HSP 1 Score: 148.7 bits (374), Expect = 9.8e-33
Identity = 84/178 (47.19%), Postives = 114/178 (64.04%), Query Frame = 1


Query: 6   LGSYSPCEDPEGPHVKEVAEWAVAEYNKGGH-CLTLIHVLSCESQVVAGVNYRLVLLVKD 65
           +G + P +DP+  HV E+ ++AV EYNK     L    V   E+QVV+G NYRL+L+VKD
Sbjct: 29  VGGWMPIKDPKEKHVMEIGQFAVTEYNKRSKSALKFESVEEGETQVVSGTNYRLILVVKD 88

Query: 66  EKNHEKVYETVVWEKIWENFRKLIYFKPYK------DPEGPHVKEIAEWAVAEYNKGGH- 125
             + +K +E VV+EK WE+F+ L  FKP        DP   HV EI ++AV EYNK    
Sbjct: 89  GPSTKK-FEAVVFEKQWEHFKSLTSFKPMFGGWTPIDPTEKHVMEIGQFAVTEYNKRSKS 148

Query: 126 CLTLISVLKCESQVVAGVNYRLVLLAKDDKNHEGKYEAVVWEKIWEHFRKLISFKPLL 176
            L   +V K  +QVV+G NYRL+L+ KD  + + K+EAVVWEK WEHF+ L SFKP++
Sbjct: 149 ALKFENVEKAWTQVVSGTNYRLILVVKDGPSTK-KFEAVVWEKPWEHFKSLTSFKPMV 204

The following BLAST results are available for this feature:

BLAST of Lsi01G011650 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CYTM_SOLTU	9.2e-23	34.07	Multicystatin OS=Solanum tuberosum PE=1 SV=1	[more]
CYT1_ACTDE	3.3e-12	45.65	Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa PE=1 SV=1	[more]
CYT5_ARATH	2.8e-11	43.53	Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana GN=CYS5 PE=2 SV=2	[more]
CYT7_ORYSJ	4.7e-11	48.19	Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica GN=Os03...	[more]
CYT8_ORYSJ	4.0e-10	40.45	Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica GN=Os03g0429000 ...	[more]

BLAST of Lsi01G011650 vs. TrEMBL


Match Name	E-value	Identity	Description
V4T622_9ROSI	4.3e-35	48.35	Cysteine proteinase inhibitor OS=Citrus clementina GN=CICLE_v10002632mg PE=3 SV=...	[more]
O80389_CUCSA	1.7e-28	70.33	Cysteine proteinase inhibitor OS=Cucumis sativus PE=2 SV=1	[more]
A0A078EQE2_BRANA	1.8e-25	40.21	Cysteine proteinase inhibitor OS=Brassica napus GN=BnaA09g19480D PE=3 SV=1	[more]
M1A5P9_SOLTU	9.9e-24	35.71	Cysteine proteinase inhibitor OS=Solanum tuberosum GN=PGSC0003DMG400005950 PE=3 ...	[more]
M4DLH7_BRARP	9.9e-24	39.15	Cysteine proteinase inhibitor OS=Brassica rapa subsp. pekinensis PE=3 SV=1	[more]

BLAST of Lsi01G011650 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G47550.1	1.6e-12	43.53	Cystatin/monellin superfamily protein	[more]
AT4G16500.1	1.5e-10	41.18	Cystatin/monellin superfamily protein	[more]
AT3G12490.2	1.0e-08	29.07	cystatin B	[more]
AT5G05110.1	5.2e-08	40.45	Cystatin/monellin family protein	[more]
AT2G40880.1	2.9e-06	36.99	cystatin A	[more]

BLAST of Lsi01G011650 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|695018835\|ref\|XP_009396392.1\|	3.6e-35	52.22	PREDICTED: cysteine proteinase inhibitor 1 {ECO:0000303\|PubMed:14697268}-like [M...	[more]
gi\|567878425\|ref\|XP_006431771.1\|	6.1e-35	48.35	hypothetical protein CICLE_v10002632mg [Citrus clementina]	[more]
gi\|568825922\|ref\|XP_006467325.1\|	6.8e-34	48.31	PREDICTED: multicystatin [Citrus sinensis]	[more]
gi\|568837934\|ref\|XP_006472972.1\|	5.7e-33	48.30	PREDICTED: multicystatin-like, partial [Citrus sinensis]	[more]
gi\|568834137\|ref\|XP_006471203.1\|	9.8e-33	47.19	PREDICTED: cysteine proteinase inhibitor 5-like [Citrus sinensis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004869	cysteine-type endopeptidase inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR027214	Cystatin
IPR020381	Proteinase inhibitor I25, cystatin, conserved region
IPR000010	Cystatin_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G011650.1	Lsi01G011650.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	SMART	SM00043	CY_4	coord: 4..93 score: 5.3E-9coord: 96..174 score: 1.
IPR020381	Proteinase inhibitor I25, cystatin, conserved region	PRODOM	PD001231		coord: 19..90 score: 2.0E-23coord: 100..171 score: 6.0
IPR020381	Proteinase inhibitor I25, cystatin, conserved region	PFAM	PF16845	SQAPI	coord: 16..92 score: 1.2E-24coord: 94..173 score: 1.1
IPR027214	Cystatin	PANTHER	PTHR11413	CYSTATIN FAMILY MEMBER	coord: 147..176 score: 5.9E-28coord: 4..97 score: 5.9
None	No IPR available	GENE3D	G3DSA:3.10.450.10		coord: 96..173 score: 2.5E-20coord: 5..92 score: 5.8
None	No IPR available	PANTHER	PTHR11413:SF26	CYSTATIN C	coord: 4..97 score: 5.9E-28coord: 147..176 score: 5.9
None	No IPR available	unknown	SSF54403	Cystatin/monellin	coord: 97..174 score: 2.34E-21coord: 4..95 score: 4.04

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi01G011650	CsGy6G025800	Cucumber (Gy14) v2	cgyblsiB398
Lsi01G011650	CsaV3_6G041410	Cucumber (Chinese Long) v3	cuclsiB462

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi01G011650	Cucumber (Gy14) v1	cgylsiB087
Lsi01G011650	Cucurbita maxima (Rimu)	cmalsiB597
Lsi01G011650	Cucurbita maxima (Rimu)	cmalsiB786
Lsi01G011650	Cucurbita moschata (Rifu)	cmolsiB582
Lsi01G011650	Cucurbita moschata (Rifu)	cmolsiB774
Lsi01G011650	Cucurbita pepo (Zucchini)	cpelsiB054
Lsi01G011650	Cucurbita pepo (Zucchini)	cpelsiB396
Lsi01G011650	Wild cucumber (PI 183967)	cpilsiB020
Lsi01G011650	Wild cucumber (PI 183967)	cpilsiB436
Lsi01G011650	Wild cucumber (PI 183967)	cpilsiB440
Lsi01G011650	Cucumber (Chinese Long) v2	culsiB021
Lsi01G011650	Cucumber (Chinese Long) v2	culsiB431
Lsi01G011650	Melon (DHL92) v3.5.1	lsimeB150
Lsi01G011650	Watermelon (Charleston Gray)	lsiwcgB127
Lsi01G011650	Watermelon (97103) v1	lsiwmB142
Lsi01G011650	Cucumber (Gy14) v2	cgyblsiB017
Lsi01G011650	Melon (DHL92) v3.6.1	lsimedB157
Lsi01G011650	Silver-seed gourd	carlsiB214
Lsi01G011650	Silver-seed gourd	carlsiB742
Lsi01G011650	Cucumber (Chinese Long) v3	cuclsiB021
Lsi01G011650	Watermelon (97103) v2	lsiwmbB114
Lsi01G011650	Watermelon (97103) v2	lsiwmbB115
Lsi01G011650	Wax gourd	lsiwgoB183