BLAST of Lsi01G009430 vs. Swiss-Prot
Match:
C81E1_GLYEC (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2)
HSP 1 Score: 394.4 bits (1012), Expect = 1.7e-108
Identity = 217/484 (44.83%), Postives = 311/484 (64.26%), Query Frame = 1
Query: 11 SLSLFFLLL--AFNLIRRSLRIRHNLPPSPPSLSIIGHLHLLKKPIHRNFQNLSVKYGHV 70
S S+F+L L FN++ R+ + + NLPP PPSL IIG+LH LK+P+HR F+ LS KYGHV
Sbjct: 8 SYSVFYLALFFIFNIVIRARKFK-NLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHV 67
Query: 71 MSLRLGSKLAVVISSSSAAEECFTKNDVVLANRPRLLIGKYIGYNYTTMVGASYGDHWRD 130
SL GS+L VV+SS+S ++CFTKNDVVLANRPR L GKYI YNYTT+ SYG+HWR+
Sbjct: 68 FSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRN 127
Query: 131 LRRIGAIEIFSPSRINKFADIRRDEVKRLVRKLSHNSINQFSKVEIQLAMSALMFNISMR 190
LRRI A+++ S RIN F+ IRRDE +RL+ +L+ +S F+++E+ + + FN MR
Sbjct: 128 LRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMR 187
Query: 191 MAAGKRYFGD--DVTNEEEARRFRELIKEIVAIAGVSNLGDFLPILNWIS-NDFEKKLTQ 250
M +GKRY+G+ D ++ +EA +FR+++ E++ ++G +N DF+P+L ++ + EK+L
Sbjct: 188 MISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKD 247
Query: 251 LGKKVDAFMQGLIDDHRRKKEEGRNTMIDHLLSLQESEPDRYNDQIIKGFIMVILTAGSD 310
+ K DAF++GLI++HR KKE NTMIDHLL+LQ+S+P+ Y DQIIKG + +L AG+D
Sbjct: 248 ISGKTDAFLRGLIEEHRTKKERA-NTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTD 307
Query: 311 SSAVTMECIISETLR----LTPATPTLVPHYAANDCTICGYDVPRDT------------- 370
SSAVT+E +S L L L H D + D+P+ T
Sbjct: 308 SSAVTLEWSMSNLLNHPEVLKKVKDELDTH-VGQDRLVDESDLPKLTYLKNVINETLRLY 367
Query: 371 ----IVLVNAWA----------------------IHRDSNEWEDPLSFKPERHEKSEAVE 430
++L ++ + IHRD W + +FKPER EK +E
Sbjct: 368 TPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKKGELE 427
Query: 431 VYKLLPFGVGRRACPGSAMAHRVMGLTLATLIQCFEWERIGEEDINMDERAGATMTKVVP 447
KL+ FG+GRRACPG +A R + +TLA LIQCF+W+ I + I++ ER G T+TK+VP
Sbjct: 428 --KLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGFTLTKLVP 486
BLAST of Lsi01G009430 vs. Swiss-Prot
Match:
C81E7_MEDTR (Isoflavone 2'-hydroxylase OS=Medicago truncatula GN=CYP81E7 PE=1 SV=1)
HSP 1 Score: 380.2 bits (975), Expect = 3.3e-104
Identity = 212/492 (43.09%), Postives = 312/492 (63.41%), Query Frame = 1
Query: 1 MDLDTYFFYSS--LSLFFLLLAFNLIRRSLRIRHNLPPSPPSLSIIGHLHLLKKPIHRNF 60
M + +Y YS LS+FF++ L+ +S + + NLPP P SL IIG+LH LK+P++R F
Sbjct: 1 MGILSYLCYSLFYLSIFFII---RLLFQSRKFK-NLPPGPTSLPIIGNLHHLKRPLNRTF 60
Query: 61 QNLSVKYGHVMSLRLGSKLAVVISSSSAAEECFTKNDVVLANRPRLLIGKYIGYNYTTMV 120
+ L+ KYG+V+SL GS+L VV+SS S +ECFTKNDVVLANRPR L GKYI YNYTT+
Sbjct: 61 KALTEKYGNVISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLG 120
Query: 121 GASYGDHWRDLRRIGAIEIFSPSRINKFADIRRDEVKRLVRKLSHNSINQFSKVEIQLAM 180
SYG+HWR+LRRI ++++ S RIN FA IRRDE +RL++KL+ +S +F++VE+
Sbjct: 121 STSYGEHWRNLRRITSLDVLSNHRINNFAPIRRDETQRLIKKLAEDSSTKFAEVELTFRF 180
Query: 181 SALMFNISMRMAAGKRYFGDD--VTNEEEARRFRELIKEIVAIAGVSNLGDFLPILNWIS 240
+ FN MRM +GKRY+GDD ++ +EA +FR+++ E++ ++G +N DF+P+L ++
Sbjct: 181 FDMTFNNIMRMISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLD 240
Query: 241 -NDFEKKLTQLGKKVDAFMQGLID-------------DHRRKKEEGR-----NTMIDHL- 300
+ EK++ ++G+K D F+ GL+ DH +E + +T+I L
Sbjct: 241 FENLEKRVKRIGEKNDVFLSGLLQEQRSKKERTNTMIDHLLNMQESQPEYYTDTIIKGLC 300
Query: 301 LSLQESEPD---------------------RYNDQIIKGFIMVILTAGSDSSAVT-MECI 360
L++ + D + D++ L SD +T + +
Sbjct: 301 LAMLLAGTDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNV 360
Query: 361 ISETLRLTPATPTLVPHYAANDCTICGYDVPRDTIVLVNAWAIHRDSNEWEDPLSFKPER 420
I ETLRL P L+PH A++C + GY VPRDTIVL+NAWAIHRD W + +FKPER
Sbjct: 361 IYETLRLYTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPER 420
Query: 421 HEKSEAVEVYKLLPFGVGRRACPGSAMAHRVMGLTLATLIQCFEWERIGEEDINMDERAG 447
+K +E K++ FG+GRRACPG +A R + +TLA L+QCF+W+RI +E I+M ER G
Sbjct: 421 FDKKGELE--KMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEKIDMSERDG 480
BLAST of Lsi01G009430 vs. Swiss-Prot
Match:
C8D11_ARATH (Cytochrome P450 81D11 OS=Arabidopsis thaliana GN=CYP81D11 PE=2 SV=1)
HSP 1 Score: 346.3 bits (887), Expect = 5.3e-94
Identity = 208/494 (42.11%), Postives = 285/494 (57.69%), Query Frame = 1
Query: 3 LDTYFFYSSLSLFFLLLAFNLIRRSLRIRHNLPPSPPS-LSIIGHLHLLKKPIHRNFQNL 62
++T + SL FF+ L+ L+ + NLPPSP IIGHLHLLK P+HR F +L
Sbjct: 8 METIYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSL 67
Query: 63 SVKYGH--VMSLRLGSKLAVVISSSSAAEECFTKNDVVLANRPRLLIGKYIGYNYTTMVG 122
S + + SL LGS+L V+SS + AEECFTKNDVVLANRP L+GK+IGYN TTMVG
Sbjct: 68 SESLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVG 127
Query: 123 ASYGDHWRDLRRIGAIEIFSPSRINKFADIRRDEVKRLVRKLSHNSINQFSKVEIQLAMS 182
A+YGD WR+LRRIG IEIFS R+N F IR+DE++RL+ L+ NS + F KVE++
Sbjct: 128 AAYGDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFM 187
Query: 183 ALMFNISMRMAAGKRYFGDDVTNEEEARRFRELI-------------------------- 242
L N +RM AGKR++GD N+ EA+ R+LI
Sbjct: 188 CLTINNIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYVTNYE 247
Query: 243 KEIVAIAGVSNLGDFLPIL------------NWISNDFEKKLTQLGKKVDAFMQGLI--- 302
K + +AG + +FL L I + + TQ D ++G+I
Sbjct: 248 KHVKKLAG--RVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQETQPDYYTDVIIKGIILVM 307
Query: 303 -----DDHRRKKEEGRNTMIDHLLSLQESEPDRYNDQIIKGFIMVILTAGSDSSAVTMEC 362
D E + +++H L++++ + +DQI + ++ ++
Sbjct: 308 ILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTE-IDDQI---GVDRLVEEQDIVKLPYLQH 367
Query: 363 IISETLRLTPATPTLVPHYAANDCTICGYDVPRDTIVLVNAWAIHRDSNEWEDPLSFKPE 422
I+SETLRL P P L+PH A+ DC + GYDVPR TI+LVNAWAIHRD WE+P FKPE
Sbjct: 368 IVSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPE 427
Query: 423 RHEKSEAVEVYKLLPFGVGRRACPGSAMAHRVMGLTLATLIQCFEWERIGEEDINMDE-R 447
R EK E KL+PFG+GRR+CPGS +A R++ L L +L+QCFEWER+ E+ ++M E
Sbjct: 428 RFEKKG--EDKKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESE 487
BLAST of Lsi01G009430 vs. Swiss-Prot
Match:
C81E8_MEDTR (Cytochrome P450 81E8 OS=Medicago truncatula GN=CYP81E8 PE=2 SV=1)
HSP 1 Score: 330.1 bits (845), Expect = 3.9e-89
Identity = 168/320 (52.50%), Postives = 229/320 (71.56%), Query Frame = 1
Query: 3 LDTYFFYSSLSLFFLLLAFNLIRRSLRIRHNLPPSPPSLSIIGHLHLLKKPIHRNFQNLS 62
+ T++ +SLFFL++ + + R NLPP P L IIG+LH LK+P+H F LS
Sbjct: 1 MTTFYLSLIISLFFLIITLKVFFNTSRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLS 60
Query: 63 VKYGHVMSLRLGSKLAVVISSSSAAEECFTKNDVVLANRPRLLIGKYIGYNYTTMVGASY 122
KYG + SL GS+L VV+SS + A+ECFTKND+VLANRP L GKYIGYN TT+ + Y
Sbjct: 61 QKYGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPY 120
Query: 123 GDHWRDLRRIGAIEIFSPSRINKFADIRRDEVKRLVRKLSHNSINQFSKVEIQLAMSALM 182
GDHWR+LRRI +IEI S R+N F +IRRDE+ RL++KL+ S N F++VE++ S +
Sbjct: 121 GDHWRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMT 180
Query: 183 FNISMRMAAGKRYFGD--DVTNEEEARRFRELIKEIVAIAGVSNLGDFLPILNWISND-F 242
FN MRM +GKRY+G+ DV++ EEAR FR +IKE+V++ G +N+GDFL L W D
Sbjct: 181 FNTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGL 240
Query: 243 EKKLTQLGKKVDAFMQGLIDDHRRKKEEGRNTMIDHLLSLQESEPDRYNDQIIKGFIMVI 302
EK+L ++ K+ DAF+QGLID+HR K NTMIDHLL+ Q+S+P+ Y DQIIKG ++V+
Sbjct: 241 EKRLKKISKRTDAFLQGLIDEHRFGKRNS-NTMIDHLLTQQQSQPEYYTDQIIKGLMVVM 300
Query: 303 LTAGSDSSAVTMECIISETL 320
L AG+D+S+VT+E +S L
Sbjct: 301 LLAGTDTSSVTIEWAMSNLL 319
BLAST of Lsi01G009430 vs. Swiss-Prot
Match:
C81E9_MEDTR (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula GN=CYP81E9 PE=1 SV=1)
HSP 1 Score: 318.9 bits (816), Expect = 9.0e-86
Identity = 163/320 (50.94%), Postives = 230/320 (71.88%), Query Frame = 1
Query: 7 FFYSSLSLFFLLLAFNLIRRSLRIRHNLPPSPPSLSIIGHLHLLKKPIHRNFQNLSVKYG 66
F+YS LSL F++ L++ + R NLPP PP++ IIG+LH LK P+HR F LS YG
Sbjct: 3 FYYSLLSLSFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYG 62
Query: 67 HVMSLRLGSKLAVVISSSSAAEECFTKNDVVLANRPRLLIGKYIGYNYTTMVGASYGDHW 126
+ SL GS+L VV+SS S A ECFTKND++LANRPR L GKYI YNYTT+ ASYGDHW
Sbjct: 63 DIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHW 122
Query: 127 RDLRRIGAIEIFSPSRINKFADIRRDEVKRLVRKLSHNSINQ---FSKVEIQLAMSALMF 186
R+LRRI I++ S +R+N F +RRDE RL++KL + +++ F+KVE++ ++ + F
Sbjct: 123 RNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTF 182
Query: 187 NISMRMAAGKRYFGD--DVTNEEEARRFRELIKEIVAIAGVSNLGDFLPILNWISND-FE 246
N MRM +GKRY+GD DV++ EEA++FRE+I E++++ G +N GDFLP+L + D E
Sbjct: 183 NAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLE 242
Query: 247 KKLTQLGKKVDAFMQGLIDDHRRKK-EEGRNTMIDHLLSLQESEPDRYNDQIIKGFIMVI 306
K+ ++ K+ +AF++GLI++HRR TMIDHLL L ES+P+ Y+D +IKG I +
Sbjct: 243 KRCKRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQGM 302
Query: 307 LTAGSDSSAVTMECIISETL 320
L AG+D+SAVT+E ++SE L
Sbjct: 303 LLAGTDTSAVTIEWVMSELL 322
BLAST of Lsi01G009430 vs. TrEMBL
Match:
A0A0A0KFV2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G492250 PE=3 SV=1)
HSP 1 Score: 525.0 bits (1351), Expect = 9.2e-146
Identity = 262/319 (82.13%), Postives = 288/319 (90.28%), Query Frame = 1
Query: 1 MDLDTYFFYSSLSLFFLLLAFNLIRRSLRIRHNLPPSPPSLSIIGHLHLLKKPIHRNFQN 60
MDLDTY YSSLSLFFLLLAF I RSLR+R+NLPPSPPS +IGHLHLLKKPIHR F N
Sbjct: 1 MDLDTYVLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPIHRTFSN 60
Query: 61 LSVKYGHVMSLRLGSKLAVVISSSSAAEECFTKNDVVLANRPRLLIGKYIGYNYTTMVGA 120
LS KYG VMSLRLGSKLAVV+SSS+A E+CFTKNDVVLANRPRLL+GKYIGYNYTTM+GA
Sbjct: 61 LSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGA 120
Query: 121 SYGDHWRDLRRIGAIEIFSPSRINKFADIRRDEVKRLVRKLSHNSINQFSKVEIQLAMSA 180
YGDHWR+LRRIGAIEIFS SRINKF DIRRDEV R+VRKLSHNSI+QFSKVEIQ AMS
Sbjct: 121 PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE 180
Query: 181 LMFNISMRMAAGKRYFGDDVTNEEEARRFRELIKEIVAIAGVSNLGDFLPILNWISNDFE 240
L FNI+MRMAAGKRY+G+DVTNEEEARRFRELIKEIVA+ GVSN GDFLP+LNW+S E
Sbjct: 181 LTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIE 240
Query: 241 KKLTQLGKKVDAFMQGLIDDHRRKKEEGRNTMIDHLLSLQESEPDRYNDQIIKGFIMVIL 300
+KL QLGKKVDAF+QGLIDDHR+ KEEGRNTMIDHLLSLQ+SEPD YNDQIIKGFI+++L
Sbjct: 241 RKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILL 300
Query: 301 TAGSDSSAVTMECIISETL 320
TAGSD+SAVTME +S L
Sbjct: 301 TAGSDTSAVTMEWALSHLL 319
BLAST of Lsi01G009430 vs. TrEMBL
Match:
A0A067KIB2_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_12476 PE=3 SV=1)
HSP 1 Score: 513.8 bits (1322), Expect = 2.1e-142
Identity = 265/485 (54.64%), Postives = 333/485 (68.66%), Query Frame = 1
Query: 7 FFYSSLSLFFLLLAFNLIRRSLRIRHNLPPSPPSLSIIGHLHLLKKPIHRNFQNLSVKYG 66
+F+SS L FL++ F S R NLPPSPPSL IIGHL+ LK PIHR +L+ KYG
Sbjct: 8 YFFSSFLLLFLVIKFY---HSKVHRKNLPPSPPSLPIIGHLYFLKPPIHRILYSLAQKYG 67
Query: 67 HVMSLRLGSKLAVVISSSSAAEECFTKNDVVLANRPRLLIGKYIGYNYTTMVGASYGDHW 126
++SLR GS+ +V+SSSS AEECFTKND+V ANRPR L+GK+I YNYTT+ A YGDHW
Sbjct: 68 PIISLRFGSRPVIVVSSSSIAEECFTKNDIVFANRPRFLLGKHIAYNYTTISQAPYGDHW 127
Query: 127 RDLRRIGAIEIFSPSRINKFADIRRDEVKRLVRKLSHNSINQFSKVEIQLAMSALMFNIS 186
R+LRR+GAIEIFS R+N F IRRDE+ RL+ KLS NS+ F KVE++ + L FNI
Sbjct: 128 RNLRRVGAIEIFSNHRLNMFLSIRRDEINRLIMKLSLNSMQDFCKVELKSMLQELTFNIM 187
Query: 187 MRMAAGKRYFGDDVTNEEEARRFRELIKEIVAIAGVSNLGDFLPILNWISNDF-EKKLTQ 246
MRM AGKRY+GDDV+NEEE + FR+L+KE++A AG SN DFLPIL+WI + +K++
Sbjct: 188 MRMVAGKRYYGDDVSNEEEGKEFRKLMKEVLAYAGASNPADFLPILHWIDGGYLKKRIIG 247
Query: 247 LGKKVDAFMQGLIDDHRRKKE--EGRNTMIDHLLSLQESEPDRYNDQIIKGFIMVILTAG 306
+ D F QGLID HR KK E +NTMIDHLLSLQESEP+ Y D+IIKG I V+L AG
Sbjct: 248 FANRFDKFFQGLIDQHRSKKGNLENKNTMIDHLLSLQESEPEYYTDEIIKGLIQVMLFAG 307
Query: 307 SDSSAVTMECII------------------------------------------SETLRL 366
+D+SAVT+E + SETLRL
Sbjct: 308 TDTSAVTLEWAMSNLLNHPSSLRKAREEIDIQMGTESLIDEPDLPTLLYLQNIISETLRL 367
Query: 367 TPATPTLVPHYAANDCTICGYDVPRDTIVLVNAWAIHRDSNEWEDPLSFKPERHEKSEAV 426
PA P L+PH ++ DC I GYDVPR T++LVNAWAIHRD W+D +SFKPER +K E
Sbjct: 368 YPAAPLLLPHVSSEDCKIEGYDVPRGTMLLVNAWAIHRDPTLWDDAMSFKPERFDKGEE- 427
Query: 427 EVYKLLPFGVGRRACPGSAMAHRVMGLTLATLIQCFEWERIGEEDINMDERAGATMTKVV 447
E KL+PFG+GRRACPG+ +A RV+GL L +LIQCFEWER+ +++++M E G+TM K
Sbjct: 428 EAKKLMPFGLGRRACPGAGLAQRVVGLALGSLIQCFEWERVSDKEVDMKEGRGSTMPKAE 487
BLAST of Lsi01G009430 vs. TrEMBL
Match:
A0A061EHP2_THECC (Cytochrome P450, family 81, subfamily D, polypeptide 8, putative OS=Theobroma cacao GN=TCM_019147 PE=3 SV=1)
HSP 1 Score: 510.0 bits (1312), Expect = 3.1e-141
Identity = 272/492 (55.28%), Postives = 341/492 (69.31%), Query Frame = 1
Query: 1 MDLDTYFFYSSLSLFFLLLAFNLIRRSLRIRHNLPPSPPSLSIIGHLHLLKKPIHRNFQN 60
M+L++ +SSLSL LL F L+ + + NLPPSPP L +GHLHL+K+PIHR + +
Sbjct: 43 MELESTILFSSLSLILLLFCFKLLFQIKTSKQNLPPSPPPLPFLGHLHLIKRPIHRFYHS 102
Query: 61 LSVKYGHVMSLRLGSKLAVVISSSSAAEECFTKNDVVLANRPRLLIGKYIGYNYTTMVGA 120
LS KYG + SLR GS+L VV+SSS+AAEECFTKND+VLA RP+LLIGKY+GYN++T+V +
Sbjct: 103 LSQKYGPIFSLRFGSRLVVVVSSSTAAEECFTKNDIVLAYRPKLLIGKYLGYNWSTVVAS 162
Query: 121 SYGDHWRDLRRIGAIEIFSPSRINKFADIRRDEVKRLVRKLS--HNSINQFSKVEIQLAM 180
YGDHWR+LRRIGAIEIFS SR+N F IR+DEVKRL+ KLS NS +F+KVE++
Sbjct: 163 PYGDHWRNLRRIGAIEIFSSSRLNAFLSIRKDEVKRLLMKLSSDDNSGQEFTKVELKSLF 222
Query: 181 SALMFNISMRMAAGKRYF-GDDVTNEEEARRFRELIKEIVAIAGVSNLGDFLPILNWI-S 240
L FN MRM AGKRY GD VT+E EA+ FREL+KE A GV++ GDFLPILNWI
Sbjct: 223 VDLTFNNIMRMVAGKRYHGGDSVTDEGEAKEFRELMKESFASGGVAHPGDFLPILNWIGG 282
Query: 241 NDFEKKLTQLGKKVDAFMQGLIDDHRRKKEEGRNTMIDHLLSLQESEPDRYNDQIIKGFI 300
+ KKL LG+++D +Q LID+ R K++ NTMIDHLLSLQ++EPD Y DQ+IKG I
Sbjct: 283 RSYLKKLMNLGERMDRLLQKLIDEVRAKRQ--GNTMIDHLLSLQQTEPDYYTDQLIKGLI 342
Query: 301 MVILTAGSDSSAVTMECIISETLR----LTPATPTL------------------------ 360
+V+L AG+D+SAVT+E +S L L A L
Sbjct: 343 LVMLLAGTDTSAVTLEWAMSNLLNNPDVLKKARTELDSQIGQENLIDEPDVSKLQYLQSI 402
Query: 361 --------------VPHYAANDCTICGYDVPRDTIVLVNAWAIHRDSNEWEDPLSFKPER 420
+PH A+ DC ICGYDVPRDTIVL+NAWAIHRD W+DP SFKPER
Sbjct: 403 IFETLRLNPSVPLLLPHMASTDCRICGYDVPRDTIVLINAWAIHRDPTLWDDPTSFKPER 462
Query: 421 HEKSEAVEVYKLLPFGVGRRACPGSAMAHRVMGLTLATLIQCFEWERIGEEDINMDERAG 447
H E E KL+PFG+GRRACPG+ +A RV+GLTL +LIQCFEWER+ E++++M E G
Sbjct: 463 HGNGER-ESNKLMPFGLGRRACPGAGLAQRVVGLTLGSLIQCFEWERVSEKEVDMAEGNG 522
BLAST of Lsi01G009430 vs. TrEMBL
Match:
A0A061EFX4_THECC (Cytochrome P450, family 81, subfamily D, polypeptide 8, putative OS=Theobroma cacao GN=TCM_019137 PE=3 SV=1)
HSP 1 Score: 506.9 bits (1304), Expect = 2.6e-140
Identity = 272/492 (55.28%), Postives = 341/492 (69.31%), Query Frame = 1
Query: 1 MDLDTYFFYSSLSLFFLLLAFNLIRRSLRIRHNLPPSPPSLSIIGHLHLLKKPIHRNFQN 60
M+ + YSSLSL LL F L+ + + NLPPSPPSL +GHLHL+K+PIHR + +
Sbjct: 1 MEPASTILYSSLSLILLLFCFKLLFQIKTSKQNLPPSPPSLPFLGHLHLIKRPIHRFYHS 60
Query: 61 LSVKYGHVMSLRLGSKLAVVISSSSAAEECFTKNDVVLANRPRLLIGKYIGYNYTTMVGA 120
LS KYG + SLR GS+L VV+SS +AA ECFTKND+VLANRP+LLIGKY+GYN++T+VG+
Sbjct: 61 LSQKYGPIFSLRFGSRLVVVVSSPTAAGECFTKNDIVLANRPKLLIGKYLGYNWSTVVGS 120
Query: 121 SYGDHWRDLRRIGAIEIFSPSRINKFADIRRDEVKRLVRKLS--HNSINQFSKVEIQLAM 180
YGDHWR+LRRI AIEIFS SR+N F IR+DEVKRL+ KLS NS +F+KVE++
Sbjct: 121 PYGDHWRNLRRISAIEIFSSSRLNAFLSIRKDEVKRLLLKLSSDDNSGQEFTKVELRSLF 180
Query: 181 SALMFNISMRMAAGKRYF-GDDVTNEEEARRFRELIKEIVAIAGVSNLGDFLPILNWI-S 240
L FN MRM AGKRY+ GD VT+E EA+ FREL+KE +A GV++ GDFLPILNWI
Sbjct: 181 VDLTFNNIMRMVAGKRYYGGDSVTDEGEAKEFRELMKESLASGGVAHPGDFLPILNWIGG 240
Query: 241 NDFEKKLTQLGKKVDAFMQGLIDDHRRKKEEGRNTMIDHLLSLQESEPDRYNDQIIKGFI 300
+ KKL LG+++D F+Q LID+ R K++ NTMIDHLLSLQ++EPD Y DQ+IKG I
Sbjct: 241 RSYLKKLMNLGERMDRFLQKLIDEVRAKRQ--GNTMIDHLLSLQQTEPDYYTDQLIKGLI 300
Query: 301 MVILTAGSDSSAVTMECIISETLR----LTPATPTL------------------------ 360
+V+L AG+D+SAVT+E +S L L A L
Sbjct: 301 LVMLLAGTDTSAVTLEWAMSNLLNNPDVLKKARTELDSQIGQENLIDEPDVSKLQYLQSI 360
Query: 361 --------------VPHYAANDCTICGYDVPRDTIVLVNAWAIHRDSNEWEDPLSFKPER 420
+PH A+ DC IC YDVPRDTIVL+NAWAIHRDS WEDP SFKPER
Sbjct: 361 IFETLRLNPAAPLLLPHMASTDCKICEYDVPRDTIVLINAWAIHRDSTLWEDPTSFKPER 420
Query: 421 HEKSEAVEVYKLLPFGVGRRACPGSAMAHRVMGLTLATLIQCFEWERIGEEDINMDERAG 447
+ E E KL+PFG+GRRACPG+ +A RV+GLTL +LIQCFEWER+ E++++M E G
Sbjct: 421 YGNGER-ESNKLMPFGLGRRACPGAGLAQRVVGLTLGSLIQCFEWERVSEKEVDMAEGDG 480
BLAST of Lsi01G009430 vs. TrEMBL
Match:
A0A0A0KJM0_CUCSA (Cytochrome P450 OS=Cucumis sativus GN=Csa_6G492230 PE=3 SV=1)
HSP 1 Score: 505.8 bits (1301), Expect = 5.8e-140
Identity = 271/482 (56.22%), Postives = 338/482 (70.12%), Query Frame = 1
Query: 9 YSSLSLFFLLLAFNLIRRSLRIRHNLPPSPPSLSIIGHLHLLKKPIHRNFQNLSVKYGHV 68
Y LSL LL AFNL R R NLPPSPPSL IIGHLH +K P HR LS KYG V
Sbjct: 8 YLFLSLISLLFAFNLRSR----RRNLPPSPPSLPIIGHLHYIKIPRHRTLHKLSAKYGPV 67
Query: 69 MSLRLGSKLAVVISSSSAAEECFTKNDVVLANRPRLLIGKYIGYNYTTMVGASYGDHWRD 128
+SL GS+L VV+SS A EECFTKND+VLANRPRLL+GK++GYN+TTMV + YGDHWR+
Sbjct: 68 ISLWFGSRLVVVVSSLPAVEECFTKNDIVLANRPRLLVGKHVGYNHTTMVASPYGDHWRN 127
Query: 129 LRRIGAIEIFSPSRINKFADIRRDEVKRLVRKLSHNSINQFSKVEIQLAMSALMFNISMR 188
LRRIGAIEIFS SR+NKFA R+DEV+RL++KLS NS+ FSKVE+Q ++S L FNISMR
Sbjct: 128 LRRIGAIEIFSASRLNKFAGTRKDEVERLLKKLSRNSMYGFSKVEMQSSISELTFNISMR 187
Query: 189 MAAGKRYFGDDVTNEEEARRFRELIKEIVAIAGVSNLGDFLPILNWISNDFEKKLTQLGK 248
MAAGKRYFG++V + EEAR+ RELIK+IV++ GVSN GDF+P++NWI N F++K+ ++ K
Sbjct: 188 MAAGKRYFGEEVRDVEEARQSRELIKQIVSMGGVSNPGDFIPMMNWIPNGFKRKVWRVAK 247
Query: 249 KVDAFMQGLID--------------DHRRKKEE------GRNTMIDHLLSLQESEPDRY- 308
++D F+QGLID DH +E G + +L L + D
Sbjct: 248 RMDGFLQGLIDEHRSNKEEERNTMIDHLLSLQETEPEYYGDQIIKGIILVLLLAGTDTSA 307
Query: 309 ------------NDQIIKGFIMVI--------LTAGSDSSAVT-MECIISETLRLTPATP 368
N +++K + L SD S + ++ IISETLRL PA P
Sbjct: 308 VTIEWALAHLLNNPEVLKKAREELDTQIGEKRLVEESDVSKLPYLQGIISETLRLNPAAP 367
Query: 369 TLVPHYAANDCTICGYDVPRDTIVLVNAWAIHRDSNEWEDPLSFKPERHEKSEAVE--VY 428
LVPH +NDCTI GY +PRDTIVLVNAWAIHRD ++WE+P FKPERH+KSE+++ +
Sbjct: 368 MLVPHLTSNDCTISGYKIPRDTIVLVNAWAIHRDPHQWEEPTLFKPERHQKSESIDHHIS 427
Query: 429 KLLPFGVGRRACPGSAMAHRVMGLTLATLIQCFEWERIGEEDINMDERAGATMTKVVPLE 447
KL+PFGVGRRACPGS MA RV+GLTLA LIQC+EWERIG+E ++M E G TM K+VPLE
Sbjct: 428 KLIPFGVGRRACPGSGMAQRVVGLTLAALIQCYEWERIGDEKVDMSEGRGVTMPKMVPLE 485
BLAST of Lsi01G009430 vs. TAIR10
Match:
AT4G37340.1 (AT4G37340.1 cytochrome P450, family 81, subfamily D, polypeptide 3)
HSP 1 Score: 444.9 bits (1143), Expect = 6.1e-125
Identity = 241/483 (49.90%), Postives = 315/483 (65.22%), Query Frame = 1
Query: 7 FFYSSLSLFFLLLAFNLIRRSLRIRHNLPPSPP-SLSIIGHLHLLKKPIHRNFQNLSVKY 66
F + SLSL F++ I+R R NLPPSP +L +IGHL LLK P+HR F ++S
Sbjct: 10 FLFISLSLTFII---GRIKR----RPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESL 69
Query: 67 GH--VMSLRLGSKLAVVISSSSAAEECFTKNDVVLANRPRLLIGKYIGYNYTTMVGASYG 126
G ++SLRLG++L V+SS S AEECFTKNDVVLANR L K+I Y TT+V ASYG
Sbjct: 70 GDAPIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYG 129
Query: 127 DHWRDLRRIGAIEIFSPSRINKFADIRRDEVKRLVRKLSHNSINQFSKVEIQLAMSALMF 186
DHWR+LRRIGA+EIFS R+N F+ IRRDE+ RL+ LS NS +F+KVE++ S L F
Sbjct: 130 DHWRNLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLTF 189
Query: 187 NISMRMAAGKRYFGDDVTNEEEARRFRELIKEIVAIAGVSNLGDFLPILNWISNDFEKKL 246
N +RM AGK Y+GD ++ EA+R RELI E + G N D+LPIL WI+ EK++
Sbjct: 190 NNIIRMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWITGS-EKRI 249
Query: 247 TQLGKKVDAFMQGLIDDHRRKKEEGRNTMIDHLLSLQESEPDRYNDQIIKGFIMVILTAG 306
++ ++D F+QGL+D+ R KE+ +NTM+DHLL LQE++P+ Y D IIKG ++ ++ AG
Sbjct: 250 KKIASRLDEFLQGLVDERREGKEKRQNTMVDHLLCLQETQPEYYTDNIIKGIMLSLILAG 309
Query: 307 SDSSAVTMEC------------------------------------------IISETLRL 366
+D+SAVT+E I+SE+LRL
Sbjct: 310 TDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQNIVSESLRL 369
Query: 367 TPATPTLVPHYAANDCTICGYDVPRDTIVLVNAWAIHRDSNEWEDPLSFKPERHEKSEAV 426
PA+P LVPH A+ DC + GY +PR T++L NAWAIHRD W+DP SFKPER EK
Sbjct: 370 YPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPERFEKEG-- 429
Query: 427 EVYKLLPFGVGRRACPGSAMAHRVMGLTLATLIQCFEWERIGEEDINMDERAGAT-MTKV 444
E KLL FG+GRRACPGS +A R+ LT+ +LIQCFEWERIGEE+++M E G M K
Sbjct: 430 EAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEGGGGVIMPKA 482
BLAST of Lsi01G009430 vs. TAIR10
Match:
AT4G37370.1 (AT4G37370.1 cytochrome P450, family 81, subfamily D, polypeptide 8)
HSP 1 Score: 380.6 bits (976), Expect = 1.4e-105
Identity = 220/477 (46.12%), Postives = 294/477 (61.64%), Query Frame = 1
Query: 13 SLFFLLLAFNLIRRSLRIRHNLPPSPP-SLSIIGHLHLLKKPIHRNFQNLSVKYGH--VM 72
S+ F++L+ + L+ + NLPPSP SL +IGHL LLK PIHR F +LS + +
Sbjct: 9 SILFVVLSLIYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNAPIF 68
Query: 73 SLRLGSKLAVVISSSSAAEECFTKNDVVLANRPRLLIGKYIGYNYTTMVGASYGDHWRDL 132
SLRLG++L V SS S AEECFTKNDVVLANRP ++ K++ Y+YTTM+ ASYGDHWR+L
Sbjct: 69 SLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWRNL 128
Query: 133 RRIGAIEIFSPSRINKFADIRRDEVKRLVRKLSHNSINQFSKVEIQLAMSALMFNISMRM 192
RRIG++EIFS R+N F IR+DE++RLV +LS N +F KV+++ +S L FN +RM
Sbjct: 129 RRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNILRM 188
Query: 193 AAGKRYFGDDVTNEEEARRFRELIKEIVAIAGVSNLGDFL-------------------- 252
AGKRY+GD V ++ EA+R R+LI ++VA AG N D+L
Sbjct: 189 VAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLVSDYETRVKKLAGRL 248
Query: 253 --PILNWISNDFEKK----------LTQLGKKVDAFMQGLIDDHRRKKE-EGRNTM---I 312
+ + E K LT + D F +I + G +T +
Sbjct: 249 DEFLQGLVDEKREAKEKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILAGTDTSAVTL 308
Query: 313 DHLLSLQESEPDRYN---DQIIKGFIMVILTAGSDSSAVT-MECIISETLRLTPATPTLV 372
+ LS + PD N D+I + + L SD S + ++ I+SETLRL PA P L+
Sbjct: 309 EWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRLYPAAPMLL 368
Query: 373 PHYAANDCTICGYDVPRDTIVLVNAWAIHRDSNEWEDPLSFKPERHEKSEAVEVYKLLPF 432
PH A+ DC + GYD+PR TI+L N WAIHRD W+DP+SFKPER EK E KL+PF
Sbjct: 369 PHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEKEG--EAQKLMPF 428
Query: 433 GVGRRACPGSAMAHRVMGLTLATLIQCFEWERIGEEDINMDERAGATMTKVVPLEVI 447
G+GRRACPGS +AHR++ LTL +LIQC EWE+IGEE ++M E G TM K PLE +
Sbjct: 429 GLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKGVTMPKAKPLEAM 482
BLAST of Lsi01G009430 vs. TAIR10
Match:
AT4G37360.1 (AT4G37360.1 cytochrome P450, family 81, subfamily D, polypeptide 2)
HSP 1 Score: 378.6 bits (971), Expect = 5.4e-105
Identity = 222/485 (45.77%), Postives = 301/485 (62.06%), Query Frame = 1
Query: 7 FFYSSLSLFFLLLAFNLIRRSLRIRHNLPPSPP-SLSIIGHLHLLKKPIHRNFQNLSVKY 66
F + LSL FL+ I+R L NLPPSP +L +IGHL LLK P+HR F ++S
Sbjct: 10 FCFIVLSLIFLI---GRIKRKL----NLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSL 69
Query: 67 GH--VMSLRLGSKLAVVISSSSAAEECFTKNDVVLANRPRLLIGKYIGYNYTTMVGASYG 126
G ++SLRLG++L V+SS S AEECFTKNDV+LANR + K+I Y +T+V ASY
Sbjct: 70 GDAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYS 129
Query: 127 DHWRDLRRIGAIEIFSPSRINKFADIRRDEVKRLVRKLSHNSINQFSKVEIQLAMSALMF 186
+HWR+LRRIGA+EIFS R+N F+ IRRDE++RL+ +L NS F+KVE++ S L F
Sbjct: 130 EHWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSDLTF 189
Query: 187 NISMRMAAGKRYFGDDVTNEEEARRFRELIKEIVAIAGVSNLGDFLPILNWISNDFEKKL 246
N +RM AGK Y+GD ++ EA+R R LI E ++ +G N D++PIL WI+ E ++
Sbjct: 190 NNIIRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS-ETRI 249
Query: 247 TQLGKKVDAFMQGLID--------------DHRRKKEEG----------RNTMIDHL--- 306
+L ++D F+QGL+D DH +E + TM+ +
Sbjct: 250 KKLAGRLDEFLQGLVDEKREGKEKKENTMVDHLLCLQETQPEYYMDRIIKGTMLSLIAGG 309
Query: 307 -----------LSLQESEPDRYN---DQIIKGFIMVILTAGSDSSAVT-MECIISETLRL 366
LS + P+ N D+I + + L SD + ++ I+SETLRL
Sbjct: 310 TDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSETLRL 369
Query: 367 TPATPTLVPHYAANDCTICGYDVPRDTIVLVNAWAIHRDSNEWEDPLSFKPERHEKSEAV 426
PA P L+PH A+ DC + GYD+PR T++L NAWAIHRD W+DP SFKPER EK
Sbjct: 370 YPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFEKEG-- 429
Query: 427 EVYKLLPFGVGRRACPGSAMAHRVMGLTLATLIQCFEWERIGEEDINMDERAGATMTKVV 447
E KL+PFG+GRRACPGS +A R++ L+L +LIQCFEWERIGEE+++M E G TM K
Sbjct: 430 EAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTMPKAR 484
BLAST of Lsi01G009430 vs. TAIR10
Match:
AT5G67310.1 (AT5G67310.1 cytochrome P450, family 81, subfamily G, polypeptide 1)
HSP 1 Score: 367.5 bits (942), Expect = 1.2e-101
Identity = 211/481 (43.87%), Postives = 288/481 (59.88%), Query Frame = 1
Query: 12 LSLFFLLLAFNLIRRSLRIRHNLPPSPPSLSIIGHLHLLKKPIHRNFQNLSVKYG-HVMS 71
L+L +A + RS + + NLPP+P +IGHLHLLK+P+HR+ ++LS G V
Sbjct: 21 LALMAGFIAVAYVFRSKQ-KKNLPPNPVGFPVIGHLHLLKEPVHRSLRDLSRNLGIDVFI 80
Query: 72 LRLGSKLAVVISSSSAAEECFTK-NDVVLANRPRLLIGKYIGYNYTTMVGASYGDHWRDL 131
LRLGS+ AVV++S+SAAEE ++ NDVV ANRP + +Y+GYN T + A YG+HWR L
Sbjct: 81 LRLGSRRAVVVTSASAAEEFLSQQNDVVFANRPLATLTEYMGYNNTLVSTAPYGEHWRRL 140
Query: 132 RRIGAIEIFSPSRINKFADIRRDEVKRLVRKLSHNSINQFSKVEIQLA--MSALMFNISM 191
RR A++I S +R+ F+DIRRDEV+ ++RK++ + V ++L + L +NI M
Sbjct: 141 RRFCAVDILSTARLRDFSDIRRDEVRAMIRKINVELVTSGGSVRLKLQPFLYGLTYNILM 200
Query: 192 RMAAGKRYFGDDVTNEEEARRFRELIKEIVAIAGVSNLGDFLPILNWISND-FEKKLTQL 251
M AGKR +EE + R+LI+E+ AGV+ +GDFLP L D + K+ +L
Sbjct: 201 SMVAGKR------EEDEETKEVRKLIREVFDFAGVNYVGDFLPTLKLFDLDGYRKRAKKL 260
Query: 252 GKKVDAFMQGLIDDHR--RKKEEGRNTMIDHLLSLQESEPDRYNDQIIKGFIMVILTAGS 311
K+D FMQ L+D+HR R K E TMI LLSLQESEP+ Y D IIKG + V+L AG+
Sbjct: 261 ASKLDKFMQKLVDEHRKNRGKAELEKTMITRLLSLQESEPECYTDDIIKGLVQVMLLAGT 320
Query: 312 DSSAVTMECIIS------ETLR------------------------------------LT 371
D++AVT+E ++ E LR L
Sbjct: 321 DTTAVTLEWAMANLLNHPEVLRKLKTELNEVSKEGRVFEESDTGKCPYLNNVISETLRLF 380
Query: 372 PATPTLVPHYAANDCTICGYDVPRDTIVLVNAWAIHRDSNEWEDPLSFKPERHEKSEAVE 431
PA P LVPH ++ DC + G+D+PR T + +NAWAI RD N W+DP +FKPER E
Sbjct: 381 PAAPLLVPHASSTDCEVAGFDIPRRTWLFINAWAIQRDPNVWDDPETFKPERFESE--TH 440
Query: 432 VYKLLPFGVGRRACPGSAMAHRVMGLTLATLIQCFEWERIGEEDINMDERAGATMTKVVP 444
K LPFG+GRRACPG +A V+ L L +LIQCF+WER + ++M E G TM K VP
Sbjct: 441 RGKFLPFGIGRRACPGMGLAQLVLSLALGSLIQCFDWERDNDVAVDMSEGKGLTMPKSVP 492
BLAST of Lsi01G009430 vs. TAIR10
Match:
AT3G28740.1 (AT3G28740.1 Cytochrome P450 superfamily protein)
HSP 1 Score: 346.3 bits (887), Expect = 3.0e-95
Identity = 208/494 (42.11%), Postives = 285/494 (57.69%), Query Frame = 1
Query: 3 LDTYFFYSSLSLFFLLLAFNLIRRSLRIRHNLPPSPPS-LSIIGHLHLLKKPIHRNFQNL 62
++T + SL FF+ L+ L+ + NLPPSP IIGHLHLLK P+HR F +L
Sbjct: 8 METIYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSL 67
Query: 63 SVKYGH--VMSLRLGSKLAVVISSSSAAEECFTKNDVVLANRPRLLIGKYIGYNYTTMVG 122
S + + SL LGS+L V+SS + AEECFTKNDVVLANRP L+GK+IGYN TTMVG
Sbjct: 68 SESLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVG 127
Query: 123 ASYGDHWRDLRRIGAIEIFSPSRINKFADIRRDEVKRLVRKLSHNSINQFSKVEIQLAMS 182
A+YGD WR+LRRIG IEIFS R+N F IR+DE++RL+ L+ NS + F KVE++
Sbjct: 128 AAYGDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFM 187
Query: 183 ALMFNISMRMAAGKRYFGDDVTNEEEARRFRELI-------------------------- 242
L N +RM AGKR++GD N+ EA+ R+LI
Sbjct: 188 CLTINNIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYVTNYE 247
Query: 243 KEIVAIAGVSNLGDFLPIL------------NWISNDFEKKLTQLGKKVDAFMQGLI--- 302
K + +AG + +FL L I + + TQ D ++G+I
Sbjct: 248 KHVKKLAG--RVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQETQPDYYTDVIIKGIILVM 307
Query: 303 -----DDHRRKKEEGRNTMIDHLLSLQESEPDRYNDQIIKGFIMVILTAGSDSSAVTMEC 362
D E + +++H L++++ + +DQI + ++ ++
Sbjct: 308 ILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTE-IDDQI---GVDRLVEEQDIVKLPYLQH 367
Query: 363 IISETLRLTPATPTLVPHYAANDCTICGYDVPRDTIVLVNAWAIHRDSNEWEDPLSFKPE 422
I+SETLRL P P L+PH A+ DC + GYDVPR TI+LVNAWAIHRD WE+P FKPE
Sbjct: 368 IVSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPE 427
Query: 423 RHEKSEAVEVYKLLPFGVGRRACPGSAMAHRVMGLTLATLIQCFEWERIGEEDINMDE-R 447
R EK E KL+PFG+GRR+CPGS +A R++ L L +L+QCFEWER+ E+ ++M E
Sbjct: 428 RFEKKG--EDKKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESE 487
BLAST of Lsi01G009430 vs. NCBI nr
Match:
gi|659079577|ref|XP_008440331.1| (PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis melo])
HSP 1 Score: 709.1 bits (1829), Expect = 5.0e-201
Identity = 366/497 (73.64%), Postives = 403/497 (81.09%), Query Frame = 1
Query: 1 MDLDTYFFYSSLSLFFLLLAFNLIRRSLRIRHNLPPSPPSLSIIGHLHLLKKPIHRNFQN 60
MDLDTY YSSLSLFFLLLAF I RSLR+R+NLPPSPPS +IGHLHLLKKP HR F N
Sbjct: 1 MDLDTYVLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPPHRTFLN 60
Query: 61 LSVKYGHVMSLRLGSKLAVVISSSSAAEECFTKNDVVLANRPRLLIGKYIGYNYTTMVGA 120
LS KYG VMSLRLGSKLAVV+SSS+A EECFTKNDVVLANRPRLLIGKYIGYNYTTMV A
Sbjct: 61 LSAKYGPVMSLRLGSKLAVVLSSSAAVEECFTKNDVVLANRPRLLIGKYIGYNYTTMVAA 120
Query: 121 SYGDHWRDLRRIGAIEIFSPSRINKFADIRRDEVKRLVRKLSHNSINQFSKVEIQLAMSA 180
YGDHWR+LRRIGAIEIFS SRINKF DIRRDEV R+VRKLSHNSI+QFSKVEIQ AMS
Sbjct: 121 PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE 180
Query: 181 LMFNISMRMAAGKRYFGDDVTNEEEARRFRELIKEIVAIAGVSNLGDFLPILNWISNDFE 240
L FNI+MRMAAGKRY+G+DVTNEEEARRFR LIKEIVA+ GVSN GDFLP+LNW+S E
Sbjct: 181 LTFNITMRMAAGKRYYGEDVTNEEEARRFRALIKEIVAVGGVSNPGDFLPVLNWMSKGIE 240
Query: 241 KKLTQLGKKVDAFMQGLIDDHRRKKEEGRNTMIDHLLSLQESEPDRYNDQIIKGFIMVIL 300
+KL QLGKKVDAF+QGLIDDHR KEEGRNTMIDHLLSLQ+SEPD YNDQIIKGFI+V+L
Sbjct: 241 RKLIQLGKKVDAFLQGLIDDHRNTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILVLL 300
Query: 301 TAGSDSSAVTMECIISETL--------------------RLT-----PATPTL------- 360
TAGSD+SAVTME + L RL P P L
Sbjct: 301 TAGSDTSAVTMEWALCHLLNNPQVLKMARDELDTQIGQERLVEESDIPKLPYLQRIIYET 360
Query: 361 ----------VPHYAANDCTICGYDVPRDTIVLVNAWAIHRDSNEWEDPLSFKPERHEKS 420
VPHYA+NDC ICGYDVPR+T+VL+NAWAIHRDSNEWED +SFKPER+EKS
Sbjct: 361 LRLNPAAPMLVPHYASNDCKICGYDVPRNTMVLINAWAIHRDSNEWEDHMSFKPERYEKS 420
Query: 421 EAVEVYKLLPFGVGRRACPGSAMAHRVMGLTLATLIQCFEWERIGEEDINMDERAGATMT 456
+A+EV+K LPFGVGRRACPGSAMAHRVMGLTLATLIQCFEW++IGEEDI+M E +GA MT
Sbjct: 421 KAMEVHKFLPFGVGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMRELSGALMT 480
BLAST of Lsi01G009430 vs. NCBI nr
Match:
gi|659079573|ref|XP_008440328.1| (PREDICTED: isoflavone 2'-hydroxylase [Cucumis melo])
HSP 1 Score: 589.3 bits (1518), Expect = 5.7e-165
Identity = 301/483 (62.32%), Postives = 363/483 (75.16%), Query Frame = 1
Query: 9 YSSLSLFFLLLAFNLIRRSLRIRHNLPPSPPSLSIIGHLHLLKKPIHRNFQNLSVKYGHV 68
Y LSL LL AFNL R S R NLPPSPPSL IIGHLH LK P+HR Q LS KYG V
Sbjct: 8 YLFLSLISLLFAFNL-RSS---RRNLPPSPPSLPIIGHLHYLKIPLHRTLQKLSAKYGPV 67
Query: 69 MSLRLGSKLAVVISSSSAAEECFTKNDVVLANRPRLLIGKYIGYNYTTMVGASYGDHWRD 128
+SL GS+L VV+SS A EECFTKND+VLANRPRLL+GK++GYN+TTMV + YGDHWR+
Sbjct: 68 ISLWFGSRLVVVVSSLPAVEECFTKNDIVLANRPRLLVGKHVGYNHTTMVASPYGDHWRN 127
Query: 129 LRRIGAIEIFSPSRINKFADIRRDEVKRLVRKLSHNSINQFSKVEIQLAMSALMFNISMR 188
LRRIGAIEIFS SR+NKFA R+DEV+RL++KLS NS+N FSKVE+Q A++ L FNISMR
Sbjct: 128 LRRIGAIEIFSASRLNKFAGTRKDEVERLLKKLSRNSMNGFSKVEMQSAIAELTFNISMR 187
Query: 189 MAAGKRYFGDDVTNEEEARRFRELIKEIVAIAGVSNLGDFLPILNWISNDFEKKLTQLGK 248
MAAGKRYFG++VT+ EEAR+ RELIK+IV++ GVSN GDF+P++NWI N F +K+++L K
Sbjct: 188 MAAGKRYFGEEVTDVEEARQSRELIKQIVSLGGVSNPGDFIPLMNWIPNGFRRKVSRLAK 247
Query: 249 KVDAFMQGLIDDHRRKKEEGRNTMIDHLLSLQESEPDRYNDQIIKGFIMVILTAGSDSSA 308
++D F+QGLID+HR K E RNTMIDHLLSLQESEP+ Y DQIIKG I+V+L AG+D+SA
Sbjct: 248 RMDGFLQGLIDEHRSNKCEERNTMIDHLLSLQESEPEYYGDQIIKGIILVLLLAGTDTSA 307
Query: 309 VTMEC------------------------------------------IISETLRLTPATP 368
VT+E IISETLRL PA P
Sbjct: 308 VTIEWALAHLLNNLEVLKKAREELDTQIGEKRLVEESDILKLPYLQGIISETLRLNPAAP 367
Query: 369 TLVPHYAANDCTICGYDVPRDTIVLVNAWAIHRDSNEWEDPLSFKPERHEKSEAV---EV 428
LVPH +NDCTI GY +PRDTIVLVNAWAIHR+ N+WE+P FKPERH+KSE+ ++
Sbjct: 368 MLVPHLTSNDCTISGYKIPRDTIVLVNAWAIHREPNQWEEPTLFKPERHQKSESSSHHQI 427
Query: 429 YKLLPFGVGRRACPGSAMAHRVMGLTLATLIQCFEWERIGEEDINMDERAGATMTKVVPL 447
KL+PFG+GRRACPGS MA RV+GLTLA LIQC+EWERIGEE ++M E G TM K++PL
Sbjct: 428 PKLIPFGIGRRACPGSGMAQRVVGLTLAALIQCYEWERIGEEKVDMAEGRGVTMPKLLPL 486
BLAST of Lsi01G009430 vs. NCBI nr
Match:
gi|778722417|ref|XP_004141999.2| (PREDICTED: cytochrome P450 81E8-like [Cucumis sativus])
HSP 1 Score: 548.5 bits (1412), Expect = 1.1e-152
Identity = 288/484 (59.50%), Postives = 354/484 (73.14%), Query Frame = 1
Query: 9 YSSLSLFFLLLAFNLIRRSLRIRHNLPPSPPSLSIIGHLHLLKKPIHRNFQNLSVKYGHV 68
Y LSL LL AFNL R R NLPPSPPSL IIGHLH LK P H+ LS +YG V
Sbjct: 8 YLFLSLISLLFAFNLRTR----RTNLPPSPPSLPIIGHLHYLKIPHHQTLHKLSAQYGPV 67
Query: 69 MSLRLGSKLAVVISSSSAAEECFTKNDVVLANRPRLLIGKYIGYNYTTMVGASYGDHWRD 128
+SL LGS+L VV+SS A EECFTKND+VLANRPRLL+GK+I YNYTTMV + YGDHWR+
Sbjct: 68 ISLWLGSRLVVVVSSLPAVEECFTKNDIVLANRPRLLVGKHISYNYTTMVSSPYGDHWRN 127
Query: 129 LRRIGAIEIFSPSRINKFADIRRDEVKRLVRKLSHNSINQFSKVEIQLAMSALMFNISMR 188
LRRIGAIEIFS SR+NKFA R+DEV+RL++KLS NS+ FSKVE+Q A+S FNISMR
Sbjct: 128 LRRIGAIEIFSASRLNKFAGSRKDEVERLLKKLSRNSMYGFSKVEMQSAISERTFNISMR 187
Query: 189 MAAGKRYFGDDVTNEEEARRFRELIKEIVAIAGVSNLGDFLPILNWISNDFEKKLTQLGK 248
MAAGKRYFG++V + EEAR+ RELIK+IV++ GVS GDF+P++NWI N F++K+ ++ K
Sbjct: 188 MAAGKRYFGEEVRDLEEARQSRELIKQIVSMGGVSYPGDFIPMMNWIPNGFKRKVWRVAK 247
Query: 249 KVDAFMQGLIDDHRRKKEEGRNTMIDHLLSLQESEPDRYNDQIIKGFIMVILTAGSDSSA 308
++DAF+QGLID+HR KEE RNT+I HLLSLQE EP+ Y D+IIKG ++++L AG D+SA
Sbjct: 248 RMDAFLQGLIDEHRSNKEEERNTVIGHLLSLQEMEPEYYGDEIIKGIVLLLL-AGVDTSA 307
Query: 309 VTMECIISETL--------------------RLTP----------------------ATP 368
VT+E I++ L RL P
Sbjct: 308 VTIEWILAHLLNNPDVLKKAREEIDAQIGEKRLVEESDIPKLPYLQGIISETLRLNPTAP 367
Query: 369 TLVPHYAANDCTICGYDVPRDTIVLVNAWAIHRDSNEWEDPLSFKPERHEK-SEAV---E 428
LVPH ++DCTI GY +PRDTIVLVNAWAIHRD N+WE+P+ FKPERH+K SE++ +
Sbjct: 368 MLVPHLTSHDCTISGYKIPRDTIVLVNAWAIHRDPNQWEEPMLFKPERHQKSSESIDHHQ 427
Query: 429 VYKLLPFGVGRRACPGSAMAHRVMGLTLATLIQCFEWERIGEEDINMDERAGATMTKVVP 447
+ KL+PFGVGRRACPGS MA RV+GLTLA LIQC+EWERIGEE ++M E GATM KV+P
Sbjct: 428 IPKLIPFGVGRRACPGSGMAQRVVGLTLAALIQCYEWERIGEEKVDMAEGRGATMPKVLP 486
BLAST of Lsi01G009430 vs. NCBI nr
Match:
gi|1009171671|ref|XP_015866864.1| (PREDICTED: cytochrome P450 81E8-like [Ziziphus jujuba])
HSP 1 Score: 535.0 bits (1377), Expect = 1.3e-148
Identity = 275/501 (54.89%), Postives = 347/501 (69.26%), Query Frame = 1
Query: 2 DLDTYFFYSSLSLFFLL-LAFNLIRRSLRIRHNLPPSPPSLSIIGHLHLLKKPIHRNFQN 61
D Y+SLS L+ + F + + NLPP PPSL IIGHLHLLK P+HR F+
Sbjct: 3 DTTLSLLYTSLSFITLVFITFKFLLSTRTPYKNLPPGPPSLPIIGHLHLLKLPVHRTFRR 62
Query: 62 LSVKYGHVMSLRLGSKLAVVISSSSAAEECFTKNDVVLANRPRLLIGKYIGYNYTTMVGA 121
LS KYG + SLR GS+ V++SS SA EECFTKND+VLANRP+LL+GK+IGYN TT+V +
Sbjct: 63 LSHKYGPIYSLRFGSRRVVIVSSPSAVEECFTKNDIVLANRPQLLVGKHIGYNNTTIVAS 122
Query: 122 SYGDHWRDLRRIGAIEIFSPSRINKFADIRRDEVKRLVRKLSHNSINQFSKVEIQLAMSA 181
YGDHWR+LRRIGAIEIFS SR+N F +R+DE+KRL+ KLS NS++ F+KVE++ S
Sbjct: 123 PYGDHWRNLRRIGAIEIFSSSRLNMFLGVRKDEIKRLLLKLSRNSVHSFAKVELKSMFSE 182
Query: 182 LMFNISMRMAAGKRYFGDDVTNEEEARRFRELIKEIVAIAGVSNLGDFLPILNWI--SND 241
L FNI MRM AGKRY+GD VT+EEEAR+FR +I+E VA G +N GDFLPILNWI S
Sbjct: 183 LTFNIVMRMGAGKRYYGDGVTHEEEARQFRAIIREAVAHGGAANPGDFLPILNWIHGSEG 242
Query: 242 FEKKLTQLGKKVDAFMQGLIDDHRRKKEEGRNTMIDHLLSLQESEPDRYNDQIIKGFIMV 301
+E ++ +L K+ DAF+QGLID+HRR E RNTMIDHLLSLQES+PD Y DQIIKG +++
Sbjct: 243 YENRVRRLAKRTDAFLQGLIDEHRR-NIESRNTMIDHLLSLQESQPDYYTDQIIKGLVLI 302
Query: 302 ILTAGSDSSAVTMECIISETLRLT------------------------------------ 361
+L AG+D+S+VT+E ++ L
Sbjct: 303 LLLAGTDTSSVTLEWAMANLLNHPHILQKARIELDEHVGQQNLIDEVDLPKLPYLKSIVS 362
Query: 362 ------PATPTLVPHYAANDCTICGYDVPRDTIVLVNAWAIHRDSNEWEDPLSFKPERHE 421
PA P LVPH+++ DC + GYDVPRDTI+LVNAWAIHRD WEDP +FKPER E
Sbjct: 363 ETLRLYPAAPMLVPHFSSEDCKVGGYDVPRDTILLVNAWAIHRDPELWEDPENFKPERFE 422
Query: 422 --KSEAVEVYKLLPFGVGRRACPGSAMAHRVMGLTLATLIQCFEWERIGEEDINMDERAG 456
K + +V+KL+PFG+GRRACPGS +A RV+GL L +LIQCFEWER+ EE+I+M E G
Sbjct: 423 NGKEDYDKVHKLVPFGIGRRACPGSGLAQRVVGLALGSLIQCFEWERVSEEEIDMSEGKG 482
BLAST of Lsi01G009430 vs. NCBI nr
Match:
gi|778718524|ref|XP_004141996.2| (PREDICTED: cytochrome P450 81E8-like [Cucumis sativus])
HSP 1 Score: 525.0 bits (1351), Expect = 1.3e-145
Identity = 262/319 (82.13%), Postives = 288/319 (90.28%), Query Frame = 1
Query: 1 MDLDTYFFYSSLSLFFLLLAFNLIRRSLRIRHNLPPSPPSLSIIGHLHLLKKPIHRNFQN 60
MDLDTY YSSLSLFFLLLAF I RSLR+R+NLPPSPPS +IGHLHLLKKPIHR F N
Sbjct: 1 MDLDTYVLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPIHRTFSN 60
Query: 61 LSVKYGHVMSLRLGSKLAVVISSSSAAEECFTKNDVVLANRPRLLIGKYIGYNYTTMVGA 120
LS KYG VMSLRLGSKLAVV+SSS+A E+CFTKNDVVLANRPRLL+GKYIGYNYTTM+GA
Sbjct: 61 LSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGA 120
Query: 121 SYGDHWRDLRRIGAIEIFSPSRINKFADIRRDEVKRLVRKLSHNSINQFSKVEIQLAMSA 180
YGDHWR+LRRIGAIEIFS SRINKF DIRRDEV R+VRKLSHNSI+QFSKVEIQ AMS
Sbjct: 121 PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE 180
Query: 181 LMFNISMRMAAGKRYFGDDVTNEEEARRFRELIKEIVAIAGVSNLGDFLPILNWISNDFE 240
L FNI+MRMAAGKRY+G+DVTNEEEARRFRELIKEIVA+ GVSN GDFLP+LNW+S E
Sbjct: 181 LTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIE 240
Query: 241 KKLTQLGKKVDAFMQGLIDDHRRKKEEGRNTMIDHLLSLQESEPDRYNDQIIKGFIMVIL 300
+KL QLGKKVDAF+QGLIDDHR+ KEEGRNTMIDHLLSLQ+SEPD YNDQIIKGFI+++L
Sbjct: 241 RKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILL 300
Query: 301 TAGSDSSAVTMECIISETL 320
TAGSD+SAVTME +S L
Sbjct: 301 TAGSDTSAVTMEWALSHLL 319
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| C81E1_GLYEC | 1.7e-108 | 44.83 | Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2 | [more] |
| C81E7_MEDTR | 3.3e-104 | 43.09 | Isoflavone 2'-hydroxylase OS=Medicago truncatula GN=CYP81E7 PE=1 SV=1 | [more] |
| C8D11_ARATH | 5.3e-94 | 42.11 | Cytochrome P450 81D11 OS=Arabidopsis thaliana GN=CYP81D11 PE=2 SV=1 | [more] |
| C81E8_MEDTR | 3.9e-89 | 52.50 | Cytochrome P450 81E8 OS=Medicago truncatula GN=CYP81E8 PE=2 SV=1 | [more] |
| C81E9_MEDTR | 9.0e-86 | 50.94 | Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula GN=CYP81E9 PE=1 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0KFV2_CUCSA | 9.2e-146 | 82.13 | Uncharacterized protein OS=Cucumis sativus GN=Csa_6G492250 PE=3 SV=1 | [more] |
| A0A067KIB2_JATCU | 2.1e-142 | 54.64 | Uncharacterized protein OS=Jatropha curcas GN=JCGZ_12476 PE=3 SV=1 | [more] |
| A0A061EHP2_THECC | 3.1e-141 | 55.28 | Cytochrome P450, family 81, subfamily D, polypeptide 8, putative OS=Theobroma ca... | [more] |
| A0A061EFX4_THECC | 2.6e-140 | 55.28 | Cytochrome P450, family 81, subfamily D, polypeptide 8, putative OS=Theobroma ca... | [more] |
| A0A0A0KJM0_CUCSA | 5.8e-140 | 56.22 | Cytochrome P450 OS=Cucumis sativus GN=Csa_6G492230 PE=3 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT4G37340.1 | 6.1e-125 | 49.90 | cytochrome P450, family 81, subfamily D, polypeptide 3 | [more] |
| AT4G37370.1 | 1.4e-105 | 46.12 | cytochrome P450, family 81, subfamily D, polypeptide 8 | [more] |
| AT4G37360.1 | 5.4e-105 | 45.77 | cytochrome P450, family 81, subfamily D, polypeptide 2 | [more] |
| AT5G67310.1 | 1.2e-101 | 43.87 | cytochrome P450, family 81, subfamily G, polypeptide 1 | [more] |
| AT3G28740.1 | 3.0e-95 | 42.11 | Cytochrome P450 superfamily protein | [more] |
