Lsi01G005010 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi01G005010
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Cytochrome P450 family 82 protein
Location	chr01 : 4112726 .. 4113315 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTTTGCGAGGCGGTGAAACAATTAGGGTAGCCGTAATATGGGCCCTCTCGCTAGTGCTAAACAATCCTCCTGTAGTAACGATCGGCAGAGGCAGAAAAGTAAAGGAATCAGATATCAATAAGCTTATGCATTTGCAAGCCGTAGTGAAAGAAACAATGCGAATATTATACGGAGCAGGAAGCTTTGTATTCGGAGAAGGCACAGAGAATTTCAGCGTAAACGGATTCCACATGCATCGAAAAGGGGACATGGGTTTAATGGAAGATTCACAGAGATGAGAATGGTTTGGGCAGAGGCATTAAAGTTTAAAGCGGAGAGATTTTTGACGACTCATAAGGAATTTGATGTGAAGGGGAAGCAATTTGAGCTGATCCCATTTGGGGGAGGAAGAAGAATATGCCATGGATTGCGTTTTGGGCTTCAAATGGTTCAGTTGATTTTGGGTAAGATTCTCCATGGGTTTGATGTAGCGACGCTGGAGAATGGGGCCGTTGATATGAAGGGGTCGATTGGACTGACAAATGTGAAAGCCAATCCATTGCAAGTTCTTATAAGGCCGCGCTTGCCTCATTCCTTATATGATTGA

mRNA sequence

ATGATTTTGCGAGGCGGTGAAACAATTAGGGTAGCCGTAATATGGGCCCTCTCGCTAGTGCTAAACAATCCTCCTGTAGTAACGATCGGCAGAGGCAGAAAAGTAAAGGAATCAGATATCAATAAGCTTATGCATTTGCAAGCCGTAGTGAAAGAAACAATGCGAATATTATACGGAGCAGGAAGCTTTGTATTCGGAGAAGGCACAGAGAATTTCAGCGGGAAGCAATTTGAGCTGATCCCATTTGGGGGAGGAAGAAGAATATGCCATGGATTGCGTTTTGGGCTTCAAATGGTTCAGTTGATTTTGGGTAAGATTCTCCATGGGTTTGATGTAGCGACGCTGGAGAATGGGGCCGTTGATATGAAGGGGTCGATTGGACTGACAAATGTGAAAGCCAATCCATTGCAAGTTCTTATAAGGCCGCGCTTGCCTCATTCCTTATATGATTGA

Coding sequence (CDS)

ATGATTTTGCGAGGCGGTGAAACAATTAGGGTAGCCGTAATATGGGCCCTCTCGCTAGTGCTAAACAATCCTCCTGTAGTAACGATCGGCAGAGGCAGAAAAGTAAAGGAATCAGATATCAATAAGCTTATGCATTTGCAAGCCGTAGTGAAAGAAACAATGCGAATATTATACGGAGCAGGAAGCTTTGTATTCGGAGAAGGCACAGAGAATTTCAGCGGGAAGCAATTTGAGCTGATCCCATTTGGGGGAGGAAGAAGAATATGCCATGGATTGCGTTTTGGGCTTCAAATGGTTCAGTTGATTTTGGGTAAGATTCTCCATGGGTTTGATGTAGCGACGCTGGAGAATGGGGCCGTTGATATGAAGGGGTCGATTGGACTGACAAATGTGAAAGCCAATCCATTGCAAGTTCTTATAAGGCCGCGCTTGCCTCATTCCTTATATGATTGA

Protein sequence

MILRGGETIRVAVIWALSLVLNNPPVVTIGRGRKVKESDINKLMHLQAVVKETMRILYGAGSFVFGEGTENFSGKQFELIPFGGGRRICHGLRFGLQMVQLILGKILHGFDVATLENGAVDMKGSIGLTNVKANPLQVLIRPRLPHSLYD

Homology

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BLAST of Lsi01G005010 vs. Swiss-Prot
Match: C82A3_SOYBN (Cytochrome P450 82A3 OS=Glycine max GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 2.4e-16
Identity = 60/206 (29.13%), Postives = 91/206 (44.17%), Query Frame = 1


Query: 1   MILRGGETIRVAVIWALSLVLNNPPVV---------TIGRGRKVKESDINKLM------- 60
           +IL G ++  V + WALSL+L NP  +          IG+   ++ESDI+KL+       
Sbjct: 320 LILGGTDSTAVTLTWALSLLLRNPLALGKAKEEIDMQIGKDEYIRESDISKLVYLQAIVK 379

Query: 61  -HLQAV---------------------VKETMRILYGAGSF-----VFGEGTE------- 120
             L+                       +K+  R+++          V+ +  E       
Sbjct: 380 ETLRLYPPAPFSSPREFTENCILGGYHIKKGTRLIHNLWKIHRDPSVWSDPLEFKPERFL 439

Query: 121 ------NFSGKQFELIPFGGGRRICHGLRFGLQMVQLILGKILHGFDVATLENGAVDMKG 151
                 +  G  FEL+PFG GRR+C G+  GL MV   L  +LH FD+       VDM  
Sbjct: 440 TTHKDVDLRGHNFELLPFGSGRRVCAGMSLGLNMVHFTLANLLHSFDILNPSAEPVDMTE 499

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BLAST of Lsi01G005010 vs. Swiss-Prot
Match: C82A2_SOYBN (Cytochrome P450 82A2 OS=Glycine max GN=CYP82A2 PE=2 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 2.7e-15
Identity = 37/80 (46.25%), Postives = 50/80 (62.50%), Query Frame = 1


Query: 71  NFSGKQFELIPFGGGRRICHGLRFGLQMVQLILGKILHGFDVATLENGAVDMKGSIGLTN 130
           +  G+ F+L+PFGGGRRIC G+  GLQ V+L L   LH F++       +DM      TN
Sbjct: 441 DMKGQHFQLLPFGGGRRICPGINLGLQTVRLTLASFLHSFEILNPSTEPLDMTEVFRATN 500

Query: 131 VKANPLQVLIRPRLPHSLYD 151
            KA PL++LI+PRL  S Y+
Sbjct: 501 TKATPLEILIKPRLSPSCYE 520

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BLAST of Lsi01G005010 vs. Swiss-Prot
Match: P6H_PAPSO (Protopine 6-monooxygenase OS=Papaver somniferum GN=CYP82N3 PE=2 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 2.7e-15
Identity = 36/78 (46.15%), Postives = 54/78 (69.23%), Query Frame = 1


Query: 74  GKQFELIPFGGGRRICHGLRFGLQMVQLILGKILHGFDVATLENGAVDMK-GSIGLTNVK 133
           G+ FEL+PFG GRR+C G+ F LQ + L+L +++HG+D+ TL    VD+   S G  N+K
Sbjct: 461 GQHFELLPFGAGRRVCPGVSFALQFMHLVLARLIHGYDMETLNGEDVDLSVSSGGHVNIK 520

Query: 134 ANPLQVLIRPRLPHSLYD 151
           + PL++++ PRL   LYD
Sbjct: 521 STPLELILTPRLHPELYD 538

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BLAST of Lsi01G005010 vs. Swiss-Prot
Match: C82A4_SOYBN (Cytochrome P450 82A4 OS=Glycine max GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 7.8e-15
Identity = 36/76 (47.37%), Postives = 48/76 (63.16%), Query Frame = 1


Query: 74  GKQFELIPFGGGRRICHGLRFGLQMVQLILGKILHGFDVATLENGAVDMKGSIGLTNVKA 133
           G  F+L+PFG GRR+C G+ FGLQ V L L   LH F++       +DM  + G+TN KA
Sbjct: 447 GHHFQLLPFGSGRRVCPGISFGLQTVHLALASFLHSFEILNPSTEPLDMTEAFGVTNTKA 506

Query: 134 NPLQVLIRPRLPHSLY 150
            PL+VL++P L  S Y
Sbjct: 507 TPLEVLVKPCLSPSCY 522

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BLAST of Lsi01G005010 vs. Swiss-Prot
Match: C82C3_ARATH (Cytochrome P450 82C3 OS=Arabidopsis thaliana GN=CYP82C3 PE=2 SV=3)

HSP 1 Score: 81.3 bits (199), Expect = 1.0e-14
Identity = 42/88 (47.73%), Postives = 55/88 (62.50%), Query Frame = 1


Query: 63  FVFGEGTE-NFSGKQFELIPFGGGRRICHGLRFGLQMVQLILGKILHGFDVATLENGAVD 122
           F+ GE  + +  G+ FEL+PFG GRR C G    +QM+ L L + LH F+V T+ +  VD
Sbjct: 424 FITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAMQMLHLGLARFLHSFEVKTVLDRPVD 483

Query: 123 MKGSIGLTNVKANPLQVLIRPRLPHSLY 150
           M  S GLT  KA PL+VLI PRL   L+
Sbjct: 484 MSESPGLTITKATPLEVLINPRLKRELF 511

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BLAST of Lsi01G005010 vs. TrEMBL
Match: I1JTF0_SOYBN (Uncharacterized protein OS=Glycine max PE=3 SV=2)

HSP 1 Score: 127.9 bits (320), Expect = 1.1e-26
Identity = 74/169 (43.79%), Postives = 98/169 (57.99%), Query Frame = 1


Query: 7   ETIRVAVIWALSLVLNNPPVVT---------IGRGRKVKESDINKLMHLQAVVKETMRI- 66
           +T  V + WALSL+LNN   +          +GR R V E DINKL++LQAVVKET+R+ 
Sbjct: 123 DTTTVTMTWALSLLLNNGDALKKVQDELDEHVGRERLVNELDINKLVYLQAVVKETLRLY 182

Query: 67  ---------LYG------AGSFVFGEGTENFSGKQFELIPFGGGRRICHGLRFGLQMVQL 126
                    LY          F+      +  G+ FELI FG GRR+C GL FGLQ++QL
Sbjct: 183 PTRPVSDPLLYSNPLEFCPERFLTTHKDIDIKGQHFELIQFGAGRRMCPGLSFGLQIMQL 242

Query: 127 ILGKILHGFDVATLENGAVDMKGSIGLTNVKANPLQVLIRPRLPHSLYD 151
               +LHGFD+ + +    DM   IGLTN+KA+PLQV++ PRL   +YD
Sbjct: 243 TPATLLHGFDIVSHDGKPTDMLEQIGLTNIKASPLQVILTPRLSTYIYD 291

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BLAST of Lsi01G005010 vs. TrEMBL
Match: M5XPI7_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa017476mg PE=3 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 2.9e-24
Identity = 70/190 (36.84%), Postives = 102/190 (53.68%), Query Frame = 1


Query: 1   MILRGGETIRVAVIWALSLVLNNPPVVT---------IGRGRKVKESDINKLMHLQAVVK 60
           +I+ G E   V + WAL+L+LNN   +          IG+GR+VKESDI  L++LQA++K
Sbjct: 227 LIVAGVEAPAVQMTWALALLLNNREALKKAQKELDQIIGKGRQVKESDIKNLVYLQAIIK 286

Query: 61  ETMRILYG-----AGS--------------------------FVFGEGTENFSGKQFELI 120
           E+   + G     AG+                          F+    + +  G  FELI
Sbjct: 287 ESTHCMVGDYHVPAGTRLLVNLSKLHRDPRVWSDPNEFRPERFLTTHKSFDVKGHDFELI 346

Query: 121 PFGGGRRICHGLRFGLQMVQLILGKILHGFDVATLENGAVDMKGSIGLTNVKANPLQVLI 151
           PFG GRR+C G+   L+++ L L  +LHGF++ T  +  VDM  ++G+TN KA PL+VL 
Sbjct: 347 PFGSGRRMCPGMSLALKVIALTLASLLHGFEIGTPTDETVDMGETVGVTNNKATPLEVLF 406

[Close]

BLAST of Lsi01G005010 vs. TrEMBL
Match: A0A067ETL4_CITSI (Uncharacterized protein (Fragment) OS=Citrus sinensis GN=CISIN_1g0450771mg PE=3 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 9.3e-23
Identity = 67/192 (34.90%), Postives = 98/192 (51.04%), Query Frame = 1


Query: 1   MILRGGETIRVAVIWALSLVLNNPPV---------VTIGRGRKVKESDINKLMHLQAVVK 60
           +IL   +T  V + W +SL+LN+  +         + +G  ++V ESDI KL++LQA++K
Sbjct: 154 LILAATDTTTVTLTWVISLLLNHRDILNKAQNELDIQVGTKKQVNESDIKKLVYLQAILK 213

Query: 61  ETMRILYGAGSFVFGEGTE----------------------------------NFSGKQF 120
           E MR+       V  E  E                                  +  G+ F
Sbjct: 214 EAMRLYPAVPLLVPHESIEECTVNGYHLHRDPRAWEEPCKFQPKRFLTRHKDIDVKGQNF 273

Query: 121 ELIPFGGGRRICHGLRFGLQMVQLILGKILHGFDVATLENGAVDMKGSIGLTNVKANPLQ 150
           EL+PF  GRR+C G+ F LQ++QL L  +LHGFD+A   N  +DM+  +GLT  K+ PL+
Sbjct: 274 ELLPFSSGRRMCPGVSFALQIMQLTLASLLHGFDIAIPSNEPLDMEEGLGLTLEKSTPLE 333

[Close]

BLAST of Lsi01G005010 vs. TrEMBL
Match: V4SE95_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10003959mg PE=3 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 9.3e-23
Identity = 67/192 (34.90%), Postives = 98/192 (51.04%), Query Frame = 1


Query: 1   MILRGGETIRVAVIWALSLVLNNPPV---------VTIGRGRKVKESDINKLMHLQAVVK 60
           +IL   +T  V + W +SL+LN+  +         + +G  ++V ESDI KL++LQA++K
Sbjct: 236 LILAATDTTTVTLTWVISLLLNHRDILNKAQNELDIQVGTKKQVNESDIKKLVYLQAILK 295

Query: 61  ETMRILYGAGSFVFGEGTE----------------------------------NFSGKQF 120
           E MR+       V  E  E                                  +  G+ F
Sbjct: 296 EAMRLYPAVPLLVPHESIEECTVNGYHLHRDPRAWEEPCKFQPKRFLTRHKDIDVKGQNF 355

Query: 121 ELIPFGGGRRICHGLRFGLQMVQLILGKILHGFDVATLENGAVDMKGSIGLTNVKANPLQ 150
           EL+PF  GRR+C G+ F LQ++QL L  +LHGFD+A   N  +DM+  +GLT  K+ PL+
Sbjct: 356 ELLPFSSGRRMCPGVSFALQIMQLTLASLLHGFDIAIPSNEPLDMEEGLGLTLEKSTPLE 415

[Close]

BLAST of Lsi01G005010 vs. TrEMBL
Match: M5VMR8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004569mg PE=3 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.0e-21
Identity = 68/174 (39.08%), Postives = 96/174 (55.17%), Query Frame = 1


Query: 1   MILRGGETIRVAVIWALSLVLNNPPV---------VTIGRGRKVKESDINKLMHLQAVVK 60
           +IL G E+  V + WALSL+LNNP           + +GR R V+ESD+  L +LQA++K
Sbjct: 329 LILTGTESTSVTLTWALSLLLNNPKTLKAAQQELDIQVGRDRWVQESDLPNLKYLQAILK 388

Query: 61  ETMRILYGAGSFVFGEGTENFSGKQFELIPFG----------------GGRRICHGLRFG 120
           ET+R+          E TE+     +  +P G                 GRR C G+  G
Sbjct: 389 ETLRVYPPGPLTGLREATEDCHLAGYH-VPKGTRVLVNIWKLQRDPRISGRRSCPGMVLG 448

Query: 121 LQMVQLILGKILHGFDVATLENGAVDMKGSIGLTNVKANPLQVLIRPRLPHSLY 150
           LQ+VQLIL +++ GFD++ +   AVDM+  +GL   KANPL+ L+ PRLP  LY
Sbjct: 449 LQVVQLILARLVQGFDMSRVGEEAVDMREGLGLALPKANPLEALLSPRLPLHLY 501

[Close]

BLAST of Lsi01G005010 vs. TAIR10
Match: AT4G31950.1 (AT4G31950.1 cytochrome P450, family 82, subfamily C, polypeptide 3)

HSP 1 Score: 81.3 bits (199), Expect = 5.7e-16
Identity = 42/88 (47.73%), Postives = 55/88 (62.50%), Query Frame = 1


Query: 63  FVFGEGTE-NFSGKQFELIPFGGGRRICHGLRFGLQMVQLILGKILHGFDVATLENGAVD 122
           F+ GE  + +  G+ FEL+PFG GRR C G    +QM+ L L + LH F+V T+ +  VD
Sbjct: 424 FITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAMQMLHLGLARFLHSFEVKTVLDRPVD 483

Query: 123 MKGSIGLTNVKANPLQVLIRPRLPHSLY 150
           M  S GLT  KA PL+VLI PRL   L+
Sbjct: 484 MSESPGLTITKATPLEVLINPRLKRELF 511

[Close]

BLAST of Lsi01G005010 vs. TAIR10
Match: AT4G31940.1 (AT4G31940.1 cytochrome P450, family 82, subfamily C, polypeptide 4)

HSP 1 Score: 79.7 bits (195), Expect = 1.7e-15
Identity = 41/88 (46.59%), Postives = 55/88 (62.50%), Query Frame = 1


Query: 63  FVFGEGTE-NFSGKQFELIPFGGGRRICHGLRFGLQMVQLILGKILHGFDVATLENGAVD 122
           F+ GE  E +  G+ FEL+PFG GRR C G    +Q++ L L + LH FDV T+ +  VD
Sbjct: 436 FITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLHSFDVKTVMDMPVD 495

Query: 123 MKGSIGLTNVKANPLQVLIRPRLPHSLY 150
           M  + GLT  KA PL+VLI PR+   L+
Sbjct: 496 MSENPGLTIPKATPLEVLISPRIKEELF 523

[Close]

BLAST of Lsi01G005010 vs. TAIR10
Match: AT4G31970.1 (AT4G31970.1 cytochrome P450, family 82, subfamily C, polypeptide 2)

HSP 1 Score: 79.3 bits (194), Expect = 2.2e-15
Identity = 42/88 (47.73%), Postives = 54/88 (61.36%), Query Frame = 1


Query: 63  FVFGEGTE-NFSGKQFELIPFGGGRRICHGLRFGLQMVQLILGKILHGFDVATLENGAVD 122
           F+ GE  E +  G+ FEL+PFG GRR C G    +Q++ L L + L  FDV T+ +  VD
Sbjct: 435 FITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQSFDVKTVMDMPVD 494

Query: 123 MKGSIGLTNVKANPLQVLIRPRLPHSLY 150
           M  S GLT  KA PL++LI PRL   LY
Sbjct: 495 MTESPGLTIPKATPLEILISPRLKEGLY 522

[Close]

BLAST of Lsi01G005010 vs. TAIR10
Match: AT2G25160.1 (AT2G25160.1 cytochrome P450, family 82, subfamily F, polypeptide 1)

HSP 1 Score: 72.0 bits (175), Expect = 3.5e-13
Identity = 36/77 (46.75%), Postives = 48/77 (62.34%), Query Frame = 1


Query: 74  GKQFELIPFGGGRRICHGLRFGLQMVQLILGKILHGFDVATLENGAVDMKGSIGLTNVKA 133
           G+ ++  PFG GRR C  +  G++MV  +L + LH FD+A   +  VDM  S GL N KA
Sbjct: 443 GQSYKFFPFGLGRRSCPAIPLGMRMVHYLLVRFLHSFDLARPSSQDVDMTESNGLVNHKA 502

Query: 134 NPLQVLIRPRLPHSLYD 151
            PL+V I PRL  SLY+
Sbjct: 503 TPLEVNIIPRLHKSLYE 519

[Close]

BLAST of Lsi01G005010 vs. TAIR10
Match: AT5G36220.1 (AT5G36220.1 cytochrome p450 81d1)

HSP 1 Score: 70.9 bits (172), Expect = 7.8e-13
Identity = 54/192 (28.12%), Postives = 89/192 (46.35%), Query Frame = 1


Query: 1   MILRGGETIRVAVIWALSLVLNNPPVVT---------IGRGRKVKESDINKLMHLQAVVK 60
           M++ G  T  V + WALS +LN+P V++         +G  R ++E+D+++L +L+ +V 
Sbjct: 303 MVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVL 362

Query: 61  ETMRILYGA--------------GSFVFGEGT-------------------ENFSGKQFE 120
           ET+R+                  GS+    GT                   ++F  ++FE
Sbjct: 363 ETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFE 422

Query: 121 -------LIPFGGGRRICHGLRFGLQMVQLILGKILHGFDVATLENGAVDMKGSIGLTNV 144
                  L+ FG GRR C G     ++V L LG ++  F+   + N  VDMK  +G T  
Sbjct: 423 KEEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTVP 482

[Close]

BLAST of Lsi01G005010 vs. NCBI nr
Match: gi|596290796|ref|XP_007226237.1| (hypothetical protein PRUPE_ppa017476mg, partial [Prunus persica])

HSP 1 Score: 119.8 bits (299), Expect = 4.1e-24
Identity = 70/190 (36.84%), Postives = 102/190 (53.68%), Query Frame = 1


Query: 1   MILRGGETIRVAVIWALSLVLNNPPVVT---------IGRGRKVKESDINKLMHLQAVVK 60
           +I+ G E   V + WAL+L+LNN   +          IG+GR+VKESDI  L++LQA++K
Sbjct: 227 LIVAGVEAPAVQMTWALALLLNNREALKKAQKELDQIIGKGRQVKESDIKNLVYLQAIIK 286

Query: 61  ETMRILYG-----AGS--------------------------FVFGEGTENFSGKQFELI 120
           E+   + G     AG+                          F+    + +  G  FELI
Sbjct: 287 ESTHCMVGDYHVPAGTRLLVNLSKLHRDPRVWSDPNEFRPERFLTTHKSFDVKGHDFELI 346

Query: 121 PFGGGRRICHGLRFGLQMVQLILGKILHGFDVATLENGAVDMKGSIGLTNVKANPLQVLI 151
           PFG GRR+C G+   L+++ L L  +LHGF++ T  +  VDM  ++G+TN KA PL+VL 
Sbjct: 347 PFGSGRRMCPGMSLALKVIALTLASLLHGFEIGTPTDETVDMGETVGVTNNKATPLEVLF 406

[Close]

BLAST of Lsi01G005010 vs. NCBI nr
Match: gi|731350022|ref|XP_010686293.1| (PREDICTED: cytochrome P450 CYP82D47-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 119.8 bits (299), Expect = 4.1e-24
Identity = 75/206 (36.41%), Postives = 104/206 (50.49%), Query Frame = 1


Query: 1   MILRGGETIRVAVIWALSLVLNNPPVV---------TIGRGRKVKESDINKLMHLQAVVK 60
           +IL   +T  + + WALSL+LNN  V+          +G+ R+V+ESD+  L++LQAV+K
Sbjct: 306 IILAATDTTAITLTWALSLLLNNKDVLKKTQAELDNVVGKERQVEESDLKNLVYLQAVLK 365

Query: 61  ETMRILYGAG---------------SFVFGEGTENF------------------------ 120
           ETMR LY AG                +    GT+ F                        
Sbjct: 366 ETMR-LYPAGPLAVPREAITDCTVNGYYIPAGTQLFVNLYKIHRDPKVWEDPLEFHPERF 425

Query: 121 ---------SGKQFELIPFGGGRRICHGLRFGLQMVQLILGKILHGFDVATLENGAVDMK 150
                     G+QFE +PFG G+RIC G+ F L  +Q  L  +LHGFD++   +  VDM 
Sbjct: 426 PTTYKQYDIRGQQFEYMPFGNGKRICPGISFALLAMQFTLASLLHGFDISIPTDETVDMT 485

[Close]

BLAST of Lsi01G005010 vs. NCBI nr
Match: gi|641838250|gb|KDO57195.1| (hypothetical protein CISIN_1g0450771mg, partial [Citrus sinensis])

HSP 1 Score: 114.8 bits (286), Expect = 1.3e-22
Identity = 67/192 (34.90%), Postives = 98/192 (51.04%), Query Frame = 1


Query: 1   MILRGGETIRVAVIWALSLVLNNPPV---------VTIGRGRKVKESDINKLMHLQAVVK 60
           +IL   +T  V + W +SL+LN+  +         + +G  ++V ESDI KL++LQA++K
Sbjct: 154 LILAATDTTTVTLTWVISLLLNHRDILNKAQNELDIQVGTKKQVNESDIKKLVYLQAILK 213

Query: 61  ETMRILYGAGSFVFGEGTE----------------------------------NFSGKQF 120
           E MR+       V  E  E                                  +  G+ F
Sbjct: 214 EAMRLYPAVPLLVPHESIEECTVNGYHLHRDPRAWEEPCKFQPKRFLTRHKDIDVKGQNF 273

Query: 121 ELIPFGGGRRICHGLRFGLQMVQLILGKILHGFDVATLENGAVDMKGSIGLTNVKANPLQ 150
           EL+PF  GRR+C G+ F LQ++QL L  +LHGFD+A   N  +DM+  +GLT  K+ PL+
Sbjct: 274 ELLPFSSGRRMCPGVSFALQIMQLTLASLLHGFDIAIPSNEPLDMEEGLGLTLEKSTPLE 333

[Close]

BLAST of Lsi01G005010 vs. NCBI nr
Match: gi|567880289|ref|XP_006432703.1| (hypothetical protein CICLE_v10003959mg [Citrus clementina])

HSP 1 Score: 114.8 bits (286), Expect = 1.3e-22
Identity = 67/192 (34.90%), Postives = 98/192 (51.04%), Query Frame = 1


Query: 1   MILRGGETIRVAVIWALSLVLNNPPV---------VTIGRGRKVKESDINKLMHLQAVVK 60
           +IL   +T  V + W +SL+LN+  +         + +G  ++V ESDI KL++LQA++K
Sbjct: 236 LILAATDTTTVTLTWVISLLLNHRDILNKAQNELDIQVGTKKQVNESDIKKLVYLQAILK 295

Query: 61  ETMRILYGAGSFVFGEGTE----------------------------------NFSGKQF 120
           E MR+       V  E  E                                  +  G+ F
Sbjct: 296 EAMRLYPAVPLLVPHESIEECTVNGYHLHRDPRAWEEPCKFQPKRFLTRHKDIDVKGQNF 355

Query: 121 ELIPFGGGRRICHGLRFGLQMVQLILGKILHGFDVATLENGAVDMKGSIGLTNVKANPLQ 150
           EL+PF  GRR+C G+ F LQ++QL L  +LHGFD+A   N  +DM+  +GLT  K+ PL+
Sbjct: 356 ELLPFSSGRRMCPGVSFALQIMQLTLASLLHGFDIAIPSNEPLDMEEGLGLTLEKSTPLE 415

[Close]

BLAST of Lsi01G005010 vs. NCBI nr
Match: gi|595795162|ref|XP_007200972.1| (hypothetical protein PRUPE_ppa004569mg [Prunus persica])

HSP 1 Score: 111.3 bits (277), Expect = 1.5e-21
Identity = 68/174 (39.08%), Postives = 96/174 (55.17%), Query Frame = 1


Query: 1   MILRGGETIRVAVIWALSLVLNNPPV---------VTIGRGRKVKESDINKLMHLQAVVK 60
           +IL G E+  V + WALSL+LNNP           + +GR R V+ESD+  L +LQA++K
Sbjct: 329 LILTGTESTSVTLTWALSLLLNNPKTLKAAQQELDIQVGRDRWVQESDLPNLKYLQAILK 388

Query: 61  ETMRILYGAGSFVFGEGTENFSGKQFELIPFG----------------GGRRICHGLRFG 120
           ET+R+          E TE+     +  +P G                 GRR C G+  G
Sbjct: 389 ETLRVYPPGPLTGLREATEDCHLAGYH-VPKGTRVLVNIWKLQRDPRISGRRSCPGMVLG 448

Query: 121 LQMVQLILGKILHGFDVATLENGAVDMKGSIGLTNVKANPLQVLIRPRLPHSLY 150
           LQ+VQLIL +++ GFD++ +   AVDM+  +GL   KANPL+ L+ PRLP  LY
Sbjct: 449 LQVVQLILARLVQGFDMSRVGEEAVDMREGLGLALPKANPLEALLSPRLPLHLY 501

The following BLAST results are available for this feature:

BLAST of Lsi01G005010 vs. Swiss-Prot


Match Name	E-value	Identity	Description
C82A3_SOYBN	2.4e-16	29.13	Cytochrome P450 82A3 OS=Glycine max GN=CYP82A3 PE=2 SV=1	[more]
C82A2_SOYBN	2.7e-15	46.25	Cytochrome P450 82A2 OS=Glycine max GN=CYP82A2 PE=2 SV=1	[more]
P6H_PAPSO	2.7e-15	46.15	Protopine 6-monooxygenase OS=Papaver somniferum GN=CYP82N3 PE=2 SV=1	[more]
C82A4_SOYBN	7.8e-15	47.37	Cytochrome P450 82A4 OS=Glycine max GN=CYP82A4 PE=2 SV=1	[more]
C82C3_ARATH	1.0e-14	47.73	Cytochrome P450 82C3 OS=Arabidopsis thaliana GN=CYP82C3 PE=2 SV=3	[more]

BLAST of Lsi01G005010 vs. TrEMBL


Match Name	E-value	Identity	Description
I1JTF0_SOYBN	1.1e-26	43.79	Uncharacterized protein OS=Glycine max PE=3 SV=2	[more]
M5XPI7_PRUPE	2.9e-24	36.84	Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa017476mg PE=3 S...	[more]
A0A067ETL4_CITSI	9.3e-23	34.90	Uncharacterized protein (Fragment) OS=Citrus sinensis GN=CISIN_1g0450771mg PE=3 ...	[more]
V4SE95_9ROSI	9.3e-23	34.90	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10003959mg PE=3 SV=1	[more]
M5VMR8_PRUPE	1.0e-21	39.08	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004569mg PE=3 SV=1	[more]

BLAST of Lsi01G005010 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G31950.1	5.7e-16	47.73	cytochrome P450, family 82, subfamily C, polypeptide 3	[more]
AT4G31940.1	1.7e-15	46.59	cytochrome P450, family 82, subfamily C, polypeptide 4	[more]
AT4G31970.1	2.2e-15	47.73	cytochrome P450, family 82, subfamily C, polypeptide 2	[more]
AT2G25160.1	3.5e-13	46.75	cytochrome P450, family 82, subfamily F, polypeptide 1	[more]
AT5G36220.1	7.8e-13	28.13	cytochrome p450 81d1	[more]

BLAST of Lsi01G005010 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|596290796\|ref\|XP_007226237.1\|	4.1e-24	36.84	hypothetical protein PRUPE_ppa017476mg, partial [Prunus persica]	[more]
gi\|731350022\|ref\|XP_010686293.1\|	4.1e-24	36.41	PREDICTED: cytochrome P450 CYP82D47-like [Beta vulgaris subsp. vulgaris]	[more]
gi\|641838250\|gb\|KDO57195.1\|	1.3e-22	34.90	hypothetical protein CISIN_1g0450771mg, partial [Citrus sinensis]	[more]
gi\|567880289\|ref\|XP_006432703.1\|	1.3e-22	34.90	hypothetical protein CICLE_v10003959mg [Citrus clementina]	[more]
gi\|595795162\|ref\|XP_007200972.1\|	1.5e-21	39.08	hypothetical protein PRUPE_ppa004569mg [Prunus persica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0020037	heme binding
GO:0005506	iron ion binding
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Vocabulary: **INTERPRO**
Term	Definition
IPR001128	Cyt_P450

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G005010.1	Lsi01G005010.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	GENE3D	G3DSA:1.10.630.10		coord: 1..57 score: 6.3E-8coord: 68..143 score: 4.1
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 69..122 score: 2.4
IPR001128	Cytochrome P450	unknown	SSF48264	Cytochrome P450	coord: 2..143 score: 3.54
None	No IPR available	PANTHER	PTHR24298	FAMILY NOT NAMED	coord: 1..147 score: 1.0
None	No IPR available	PANTHER	PTHR24298:SF102	SUBFAMILY NOT NAMED	coord: 1..147 score: 1.0

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi01G005010	Watermelon (97103) v2	lsiwmbB113
Lsi01G005010	Watermelon (97103) v2	lsiwmbB115
Lsi01G005010	Wax gourd	lsiwgoB168
Lsi01G005010	Bottle gourd (USVL1VR-Ls)	lsilsiB065
Lsi01G005010	Cucumber (Gy14) v1	cgylsiB022
Lsi01G005010	Cucurbita maxima (Rimu)	cmalsiB367
Lsi01G005010	Cucurbita maxima (Rimu)	cmalsiB517
Lsi01G005010	Cucurbita maxima (Rimu)	cmalsiB597
Lsi01G005010	Cucurbita maxima (Rimu)	cmalsiB786
Lsi01G005010	Cucurbita moschata (Rifu)	cmolsiB502
Lsi01G005010	Cucurbita moschata (Rifu)	cmolsiB582
Lsi01G005010	Cucurbita moschata (Rifu)	cmolsiB774
Lsi01G005010	Cucurbita pepo (Zucchini)	cpelsiB054
Lsi01G005010	Cucurbita pepo (Zucchini)	cpelsiB396
Lsi01G005010	Cucurbita pepo (Zucchini)	cpelsiB413
Lsi01G005010	Wild cucumber (PI 183967)	cpilsiB019
Lsi01G005010	Wild cucumber (PI 183967)	cpilsiB439
Lsi01G005010	Cucumber (Chinese Long) v2	culsiB020
Lsi01G005010	Cucumber (Chinese Long) v2	culsiB434
Lsi01G005010	Melon (DHL92) v3.5.1	lsimeB129
Lsi01G005010	Melon (DHL92) v3.5.1	lsimeB156
Lsi01G005010	Watermelon (Charleston Gray)	lsiwcgB125
Lsi01G005010	Watermelon (Charleston Gray)	lsiwcgB127
Lsi01G005010	Watermelon (97103) v1	lsiwmB144
Lsi01G005010	Cucumber (Gy14) v2	cgyblsiB014
Lsi01G005010	Cucumber (Gy14) v2	cgyblsiB016
Lsi01G005010	Cucumber (Gy14) v2	cgyblsiB398
Lsi01G005010	Melon (DHL92) v3.6.1	lsimedB163
Lsi01G005010	Silver-seed gourd	carlsiB364
Lsi01G005010	Silver-seed gourd	carlsiB585
Lsi01G005010	Cucumber (Chinese Long) v3	cuclsiB017
Lsi01G005010	Cucumber (Chinese Long) v3	cuclsiB462