Lsi01G003330 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi01G003330
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionEpoxide hydrolase 2
Locationchr01 : 2916098 .. 2919206 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAGAATTAACCATTTAATTAAAGAAAAAAATCTTTTGATTGATTTTGTTCTCTTAAAGAGTAAATGATAAATAATTATCCGCTGAGAATTCGACCACCACAAATTCTTTACCAAGAATGTTCACCTCAATCTTTGCAAAATTATTCATAATTTTATCGAAATAGAGAATGAAATGAGTGGCAGGAAGGGAAAGGGATGGACAAATCATTCTCATTCCCCCTTGACATCTCTAAAACAAACCCCATAGTTATTGGTTATTTTTTATAATTTAATTATATGTGTTTTTAATGGGTAATTTTTTGTATTATTAATTTAAATTGTTTGAAATGTTATGGGCTAAACTTAAGAAAATATTTATAGGCTAATATTATGCAGAAGATTTCTTTATTATTATAATCGTTACAATTCCTTTCAACTTTAGCGAACAACTTTCGTCATTTTTTAAAGTTAAATTACAGGGGAAAAAATGTAGTCCTAGTTTTTGCAATTTTGTATTTAACAGTAATAAACTTCCATAAAAATACTAAAATGATGACAATAAATAGAACACTGAAAAGTCTCCGTTCGGTTTTTTCGGAACACCGCCAACAAAGTCAACCCGCCCACAAACATCGACTTTATTTATTTATTTAATCATTCACTGAGCGGCAGTTGGTTCGAAAATTGAAGCAGAGCAGAGTTCAAAATATCTTCGCCGCTCGCTCACTCATTTCCCTCAAATGGATCGAATTCAGCACAATTTCATCGAAGTTGGAGGCCTAAAGCTCCATGTAGCCGAGATCGGAACTGGTGATCTCTCATCTCATCGTCAATTTCTTCACCGGAGCTTGTCTTGCTTACAAATACCTACTCTCTGTCTGTTTGTGCTGCTCCTTTTCCCATTTTTCTTTCAGGTTCCAATGTCGTTGTCTTCTTACACGGATTTCCGGAGATATGGTACTCATGGCGGTACCAGATGATTGCTCTTGCTGACGCCGGCTTCCGAGTACTCGCTCCCGATTACAGAGGATATGGACTCTCCGATTCCCCCGCCGAGCCCTCCAAGGCTTCATTCAACGACCTCATCAGTGATCTCCTTGGGATTCTTGACGCTCTCAACATCCCGAAGGTACTATTCTCCTTAGCATGCGCTTTGATTTGGTTGTAATCGTATTGAAGTTAATAGGGTTCCACTGAAGATTGCATTACATTGACTGCGAGGTGTTTGAAAAAATGAACTGCTGGATGAATTGAGTAATAGGGAAAAAGTGTAAAACATATTGATGTTCATTGTATCAAACCAGGCATATTAACCTTTGATTTTATGATTTTAAGTTAACGGCATTCCAACATAGTTAGTCGCATTGATATATATGGCAAACTAAGGAATCTTCAAACATTACTTTGTATAGGTGTTTGTTGTTGCGAAAGATTTTGGAGCATGGCCAGCTTATTACTTTGCATTGAAACATCCTGAAAGGGCATTGGGAATTGTCACATTGGGAGTGCCATTCCTTCCTCCTGAATCTGTTAAGAGTTCCCAAAGCAACATCCCTGAAGGCGTGTATACTTTAAGATGGCGGGTATGCACATGCTCTACCATTGCCTTACACTCTCCCCTTTCAAAAGGAAACTGAATGGAAGTATTCCAAGTGTGTATTATTTCAATGCTTTGGACACGAATGTATTGTTTGTTGAAAGAAACAATTAGTTTCAAGTTGTTTTTGTTAGAGTGTTGATCTATTATTAAATTTACCTTTTACCTATTAGCTTAAGCTTTTGGGTTAATCAATGATTTAACATAGTATTACAGTAGGTGCTCCTGAGTTTAAAACCTTGCCGTGTCTTATTTTCTCTCCAATTAATATTGATTTCCACTTGTTGGGTCTTCTAATTAAAAGTGAGGGGAGTGTTAGAGTGTTGATATAATATTAAATTTAACTTCTTCTATTTTGGGTCAATTGGTGATTTAATAGTTTTTACTTTTTTAACAAATGGACATTCCTACTATGAGTCTGTGACTATTGATTTTACAGTATTGATTCTGGATAGAGATTTCTATCTCAATAGTTAATAAGCAAATCATAGTTCTTCTACAGAATAAATGAATCTTTTATCATGTTAGGTTGGATTTCAAGTTTCTTAACTTTGTTTGCCTGTTTGTTCTTTTCTTTTACTAACTTGTTGCTTCAACTTTCTCTCTACTCAAGGAACCCGGGCGAGCTGAAGCTGATTTTGGTCGTTTTGATGCAAAAACTGTTGTCAGGAATGTTTATATCCTCTTCTCCAAAAGTGAAATACCAATAGCTCAAGAAAATCAAGAAGTAATGGACTTAGTAGACCCATCCACCCCTCTTCCTCCTTGGTTCACTGAGGAAGATCTAGCAACTTATGGAGCTCTCTATGAGAAATCTGGATTTGATACTGCATTGAAAATTCCTTACAGGTATGGCACTTGAGTTATGAGATGATCACATCTTGTTTTACTTGGATAATTCTGTTACATTTGATTATGATGGATTGATTCACATGCATGATAGGTCATTCAATGAAGATTTGGGAATAAAGGATCCAAAAGTTGAAATTCCAGCACTCTTTATAATGGGTGAAAAGGACTATGTTCTCAAATTCCCTGAAATTGAGGAATACGTAAGAAGCGAAAGAGTTAAAGATTTTGTTCCCAAATTGGAGATTATCTTTTTACCAGAAGGATCTCATTTTGTTCAGGAGCAATCTCCCGAAGAAGTTAATCAGCTACTACTTGCTTTCCTTGCCAAGCATATTTGACGGTGAACACATTATCAACACTCCAAGTTTTAAATCTTAATAAAACTCAATATGACTCTTTCTGTGTACTTTGTTCTTAAATTCAATCAATAGTTACAATAGGTAGGTTATTATTATTATTATTATTATTATTATTATTATTATTTTGAGTTAAACATGGTGGAGATTCCAATTAACATCAAACCTTTTACTTAAGAGTACACCGTTTATGTTTACTTTGACACGATATATATCTTATTTGGGGAGCTAGACGTTTGTAAACTTTATAGTTGTCTACTTGAAATGTCAGAGGGACTTTCGTCCAAACTAAAAGGATTTATGTATAAAATGTTATGTGAGATC

mRNA sequence

AGAGAATTAACCATTTAATTAAAGAAAAAAATCTTTTGATTGATTTTGTTCTCTTAAAGAGTAAATGATAAATAATTATCCGCTGAGAATTCGACCACCACAAATTCTTTACCAAGAATGTTCACCTCAATCTTTGCAAAATTATTCATAATTTTATCGAAATAGAGAATGAAATGAGTGGCAGGAAGGGAAAGGGATGGACAAATCATTCTCATTCCCCCTTGACATCTCTAAAACAAACCCCATAGTTATTGGTTATTTTTTATAATTTAATTATATGTGTTTTTAATGGGTAATTTTTTGTATTATTAATTTAAATTGTTTGAAATGTTATGGGCTAAACTTAAGAAAATATTTATAGGCTAATATTATGCAGAAGATTTCTTTATTATTATAATCGTTACAATTCCTTTCAACTTTAGCGAACAACTTTCGTCATTTTTTAAAGTTAAATTACAGGGGAAAAAATGTAGTCCTAGTTTTTGCAATTTTGTATTTAACAGTAATAAACTTCCATAAAAATACTAAAATGATGACAATAAATAGAACACTGAAAAGTCTCCGTTCGGTTTTTTCGGAACACCGCCAACAAAGTCAACCCGCCCACAAACATCGACTTTATTTATTTATTTAATCATTCACTGAGCGGCAGTTGGTTCGAAAATTGAAGCAGAGCAGAGTTCAAAATATCTTCGCCGCTCGCTCACTCATTTCCCTCAAATGGATCGAATTCAGCACAATTTCATCGAAGTTGGAGGCCTAAAGCTCCATGTAGCCGAGATCGGAACTGGTTCCAATGTCGTTGTCTTCTTACACGGATTTCCGGAGATATGGTACTCATGGCGGTACCAGATGATTGCTCTTGCTGACGCCGGCTTCCGAGTACTCGCTCCCGATTACAGAGGATATGGACTCTCCGATTCCCCCGCCGAGCCCTCCAAGGCTTCATTCAACGACCTCATCAGTGATCTCCTTGGGATTCTTGACGCTCTCAACATCCCGAAGGTGTTTGTTGTTGCGAAAGATTTTGGAGCATGGCCAGCTTATTACTTTGCATTGAAACATCCTGAAAGGGCATTGGGAATTGTCACATTGGGAGTGCCATTCCTTCCTCCTGAATCTGTTAAGAGTTCCCAAAGCAACATCCCTGAAGGCGTGTATACTTTAAGATGGCGGGAACCCGGGCGAGCTGAAGCTGATTTTGGTCGTTTTGATGCAAAAACTGTTGTCAGGAATGTTTATATCCTCTTCTCCAAAAGTGAAATACCAATAGCTCAAGAAAATCAAGAAGTAATGGACTTAGTAGACCCATCCACCCCTCTTCCTCCTTGGTTCACTGAGGAAGATCTAGCAACTTATGGAGCTCTCTATGAGAAATCTGGATTTGATACTGCATTGAAAATTCCTTACAGGTCATTCAATGAAGATTTGGGAATAAAGGATCCAAAAGTTGAAATTCCAGCACTCTTTATAATGGGTGAAAAGGACTATGTTCTCAAATTCCCTGAAATTGAGGAATACGTAAGAAGCGAAAGAGTTAAAGATTTTGTTCCCAAATTGGAGATTATCTTTTTACCAGAAGGATCTCATTTTGTTCAGGAGCAATCTCCCGAAGAAGTTAATCAGCTACTACTTGCTTTCCTTGCCAAGCATATTTGACGGTGAACACATTATCAACACTCCAAGTTTTAAATCTTAATAAAACTCAATATGACTCTTTCTGTGTACTTTGTTCTTAAATTCAATCAATAGTTACAATAGGTAGGTTATTATTATTATTATTATTATTATTATTATTATTATTTTGAGTTAAACATGGTGGAGATTCCAATTAACATCAAACCTTTTACTTAAGAGTACACCGTTTATGTTTACTTTGACACGATATATATCTTATTTGGGGAGCTAGACGTTTGTAAACTTTATAGTTGTCTACTTGAAATGTCAGAGGGACTTTCGTCCAAACTAAAAGGATTTATGTATAAAATGTTATGTGAGATC

Coding sequence (CDS)

ATGGATCGAATTCAGCACAATTTCATCGAAGTTGGAGGCCTAAAGCTCCATGTAGCCGAGATCGGAACTGGTTCCAATGTCGTTGTCTTCTTACACGGATTTCCGGAGATATGGTACTCATGGCGGTACCAGATGATTGCTCTTGCTGACGCCGGCTTCCGAGTACTCGCTCCCGATTACAGAGGATATGGACTCTCCGATTCCCCCGCCGAGCCCTCCAAGGCTTCATTCAACGACCTCATCAGTGATCTCCTTGGGATTCTTGACGCTCTCAACATCCCGAAGGTGTTTGTTGTTGCGAAAGATTTTGGAGCATGGCCAGCTTATTACTTTGCATTGAAACATCCTGAAAGGGCATTGGGAATTGTCACATTGGGAGTGCCATTCCTTCCTCCTGAATCTGTTAAGAGTTCCCAAAGCAACATCCCTGAAGGCGTGTATACTTTAAGATGGCGGGAACCCGGGCGAGCTGAAGCTGATTTTGGTCGTTTTGATGCAAAAACTGTTGTCAGGAATGTTTATATCCTCTTCTCCAAAAGTGAAATACCAATAGCTCAAGAAAATCAAGAAGTAATGGACTTAGTAGACCCATCCACCCCTCTTCCTCCTTGGTTCACTGAGGAAGATCTAGCAACTTATGGAGCTCTCTATGAGAAATCTGGATTTGATACTGCATTGAAAATTCCTTACAGGTCATTCAATGAAGATTTGGGAATAAAGGATCCAAAAGTTGAAATTCCAGCACTCTTTATAATGGGTGAAAAGGACTATGTTCTCAAATTCCCTGAAATTGAGGAATACGTAAGAAGCGAAAGAGTTAAAGATTTTGTTCCCAAATTGGAGATTATCTTTTTACCAGAAGGATCTCATTTTGTTCAGGAGCAATCTCCCGAAGAAGTTAATCAGCTACTACTTGCTTTCCTTGCCAAGCATATTTGA

Protein sequence

MDRIQHNFIEVGGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDYRGYGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERALGIVTLGVPFLPPESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKSEIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFNEDLGIKDPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSHFVQEQSPEEVNQLLLAFLAKHI
BLAST of Lsi01G003330 vs. Swiss-Prot
Match: HYES_HUMAN (Bifunctional epoxide hydrolase 2 OS=Homo sapiens GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 159.5 bits (402), Expect = 6.1e-38
Identity = 107/321 (33.33%), Postives = 166/321 (51.71%), Query Frame = 1

Query: 4   IQHNFIEVGG-LKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDYRG 63
           + H ++ V   ++LH  E+G+G   V   HGFPE WYSWRYQ+ ALA AG+RVLA D +G
Sbjct: 237 MSHGYVTVKPRVRLHFVELGSGP-AVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKG 296

Query: 64  YGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERALGI 123
           YG S +P E  +     L  +++  LD L + +   +  D+G    +Y AL +PER   +
Sbjct: 297 YGESSAPPEIEEYCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAV 356

Query: 124 VTLGVPFLPPESVKSSQSNI---PEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSK 183
            +L  PF+P     S   +I   P   Y L ++EPG AEA+      + + R    LF  
Sbjct: 357 ASLNTPFIPANPNMSPLESIKANPVFDYQLYFQEPGVAEAEL----EQNLSRTFKSLFRA 416

Query: 184 SEIPIAQENQEV----MDLVDPSTP-LPPWFTEEDLATYGALYEKSGFDTALKIPYRSFN 243
           S+  +   ++      + +  P  P L    TEE++  Y   ++KSGF   L   YR+  
Sbjct: 417 SDESVLSMHKVCEAGGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNW-YRNME 476

Query: 244 -------EDLGIKDPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPE 303
                  + LG    K+ IPAL +  EKD+VL  P++     S+ ++D++P L+   + +
Sbjct: 477 RNWKWACKSLG---RKILIPALMVTAEKDFVL-VPQM-----SQHMEDWIPHLKRGHIED 536

Query: 304 GSHFVQEQSPEEVNQLLLAFL 309
             H+ Q   P EVNQ+L+ +L
Sbjct: 537 CGHWTQMDKPTEVNQILIKWL 542

BLAST of Lsi01G003330 vs. Swiss-Prot
Match: HYES_RAT (Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus GN=Ephx2 PE=1 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 6.1e-38
Identity = 107/322 (33.23%), Postives = 167/322 (51.86%), Query Frame = 1

Query: 4   IQHNFIEVG-GLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDYRG 63
           + H ++ V  G++LH  E+G+G   +   HGFPE W+SWRYQ+ ALA AGFRVLA D +G
Sbjct: 235 VSHGYVTVKPGIRLHFVEMGSGP-AICLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKG 294

Query: 64  YGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERALGI 123
           YG S SP E  + +   L  +++  L+ L IP+   +  D+     +  AL HPER   +
Sbjct: 295 YGDSSSPPEIEEYAMELLCEEMVTFLNKLGIPQAVFIGHDWAGVLVWNMALFHPERVRAV 354

Query: 124 VTLGVPFLPPESVKSSQS---NIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSK 183
            +L  P +PP    S      +IP   Y L ++EPG AEA+      K + R     F  
Sbjct: 355 ASLNTPLMPPNPEVSPMEVIRSIPVFNYQLYFQEPGVAEAEL----EKNMSRTFKSFFRT 414

Query: 184 S-EIPIAQENQEV-MDLVDPSTPLPPWF----TEEDLATYGALYEKSGFDTAL---KIPY 243
           S ++ +   N+   M  +   TP  P      TEE++  Y   ++KSGF   L   +   
Sbjct: 415 SDDMGLLTVNKATEMGGILVGTPEDPKVSKITTEEEIEYYIQQFKKSGFRGPLNWYRNTE 474

Query: 244 RSFNEDLGIKDPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSH 303
           R++         K+ +PAL +  EKD VL+ PE+     S+ +++++P L+   + +  H
Sbjct: 475 RNWKWSCKALGRKILVPALMVTAEKDIVLR-PEM-----SKNMENWIPFLKRGHIEDCGH 534

Query: 304 FVQEQSPEEVNQLLLAFLAKHI 313
           + Q + P EVNQ+L+ +L   I
Sbjct: 535 WTQIEKPAEVNQILIKWLKTEI 545

BLAST of Lsi01G003330 vs. Swiss-Prot
Match: HYES_MOUSE (Bifunctional epoxide hydrolase 2 OS=Mus musculus GN=Ephx2 PE=1 SV=2)

HSP 1 Score: 159.1 bits (401), Expect = 8.0e-38
Identity = 102/319 (31.97%), Postives = 170/319 (53.29%), Query Frame = 1

Query: 4   IQHNFIEVG-GLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDYRG 63
           + H ++ V  G++LH  E+G+G   +   HGFPE W+SWRYQ+ ALA AGFRVLA D +G
Sbjct: 235 VSHGYVTVKPGIRLHFVEMGSGP-ALCLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKG 294

Query: 64  YGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERALGI 123
           YG S SP E  + +   L  +++  LD L IP+   +  D+     +  AL +PER   +
Sbjct: 295 YGDSSSPPEIEEYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERVRAV 354

Query: 124 VTLGVPFLPPESVKSSQS---NIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSK 183
            +L  PF+PP+   S      +IP   Y L ++EPG AEA+  +  ++T  ++ +    +
Sbjct: 355 ASLNTPFMPPDPDVSPMKVIRSIPVFNYQLYFQEPGVAEAELEKNMSRT-FKSFFRASDE 414

Query: 184 SEIPIAQENQEVMDLV--DPSTP-LPPWFTEEDLATYGALYEKSGFDTAL---KIPYRSF 243
           +      +  E+  ++   P  P L    TEE++  Y   ++K+GF   L   +   R++
Sbjct: 415 TGFIAVHKATEIGGILVNTPEDPNLSKITTEEEIEFYIQQFKKTGFRGPLNWYRNTERNW 474

Query: 244 NEDLGIKDPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSHFVQ 303
                    K+ +PAL +  EKD VL+ PE+     S+ ++ ++P L+   + +  H+ Q
Sbjct: 475 KWSCKGLGRKILVPALMVTAEKDIVLR-PEM-----SKNMEKWIPFLKRGHIEDCGHWTQ 534

Query: 304 EQSPEEVNQLLLAFLAKHI 313
            + P EVNQ+L+ +L   +
Sbjct: 535 IEKPTEVNQILIKWLQTEV 545

BLAST of Lsi01G003330 vs. Swiss-Prot
Match: HYES_PIG (Bifunctional epoxide hydrolase 2 OS=Sus scrofa GN=EPHX2 PE=2 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 1.8e-34
Identity = 102/315 (32.38%), Postives = 164/315 (52.06%), Query Frame = 1

Query: 4   IQHNFIEVG-GLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDYRG 63
           + H ++ +  G++LH  E+G+G   V   HGFPE W+SWRYQ+ ALA AGFRVLA D +G
Sbjct: 237 LSHGYVLIKPGVRLHFVEMGSGP-AVCLCHGFPESWFSWRYQIPALAQAGFRVLAVDMKG 296

Query: 64  YGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERALGI 123
           YG S +P E  + S   L  D++  L+ L + +   +  D+G    +  AL +PER   +
Sbjct: 297 YGESSAPPEIEEYSLEVLCKDMVTFLNKLGLSQAVFIGHDWGGVLVWNMALFYPERVRAV 356

Query: 124 VTLGVPFLPPESVKSSQSNI---PEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSK 183
            +L  PF+P     S    I   P   Y L ++EPG AEA+  + +     +N +    +
Sbjct: 357 ASLNTPFMPSNPNVSPMEIIKANPVFDYQLYFQEPGVAEAELEQ-NLDRTFKNFFRAHDE 416

Query: 184 SEIPIAQENQ-EVMDLVDPSTP-LPPWFTEEDLATYGALYEKSGFDTALKIPYRSF--NE 243
           + +   +  +   + +  P  P L    TEED+  Y   ++KSGF   L   YR+   N 
Sbjct: 417 TFLTTNRVRELGGLFVGTPEEPSLSRLVTEEDIQFYVQQFKKSGFRGPLNW-YRNMERNW 476

Query: 244 DLGIKDP--KVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSHFVQ 303
             G K    K+ IPAL +  E D VL  P++     S+ +++++P L+   + +  H+ Q
Sbjct: 477 QWGCKGSGRKILIPALMVTAENDLVL-HPKM-----SKHMENWIPHLKRGHIKDCGHWTQ 536

Query: 304 EQSPEEVNQLLLAFL 309
              P E+N++L+ +L
Sbjct: 537 IDKPAELNRILIEWL 542

BLAST of Lsi01G003330 vs. Swiss-Prot
Match: YFHM_BACSU (AB hydrolase superfamily protein YfhM OS=Bacillus subtilis (strain 168) GN=yfhM PE=3 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.1e-31
Identity = 92/320 (28.75%), Postives = 143/320 (44.69%), Query Frame = 1

Query: 1   MDRIQHNFIEVGGLKLHVAEIGTGSN-VVVFLHGFPEIWYSWRYQMIALADAGFRVLAPD 60
           MD ++  F+   G+ LHVA  G     ++V LHGFPE WY W+ Q+  L DAG+RV+APD
Sbjct: 1   MDGVKCQFVNTNGITLHVAAAGREDGPLIVLLHGFPEFWYGWKNQIKPLVDAGYRVIAPD 60

Query: 61  YRGYGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERA 120
            RGY LSD P        + L  D++G++      K  V+  D+G   A++ A   PE  
Sbjct: 61  QRGYNLSDKPEGIDSYRIDTLRDDIIGLITQFTDEKAIVIGHDWGGAVAWHLASTRPEYL 120

Query: 121 LGIVTLGV----------PFLPPESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTV 180
             ++ + +          P  PP+ +KSS        Y   ++ P   EA     D  T+
Sbjct: 121 EKLIAINIPHPHVMKTVTPLYPPQWLKSS--------YIAYFQLPDIPEASLRENDYDTL 180

Query: 181 VRNVYILFSKSEIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIP 240
            + + +                       +  P  FT ED++ Y   +++ G  TA+   
Sbjct: 181 DKAIGL-----------------------SDRPALFTSEDVSRYKEAWKQPGALTAMLNW 240

Query: 241 YRSFNEDLGIKDPKVE-IPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEG 300
           YR+  +    + P  E +P   I G +D  L     +E  R        P   +IF+ E 
Sbjct: 241 YRALRKGSLAEKPSYETVPYRMIWGMEDRFLSRKLAKETER------HCPNGHLIFVDEA 283

Query: 301 SHFVQEQSPEEVNQLLLAFL 309
           SH++  + P  VNQL+L +L
Sbjct: 301 SHWINHEKPAIVNQLILEYL 283

BLAST of Lsi01G003330 vs. TrEMBL
Match: A0A0A0KHK9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G517180 PE=4 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 1.0e-172
Identity = 296/312 (94.87%), Postives = 302/312 (96.79%), Query Frame = 1

Query: 1   MDRIQHNFIEVGGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY 60
           MDRI HNFIEVG LKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY
Sbjct: 1   MDRIHHNFIEVGALKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY 60

Query: 61  RGYGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL 120
           RGYGLSDSPAEPSKASF+DLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL
Sbjct: 61  RGYGLSDSPAEPSKASFSDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL 120

Query: 121 GIVTLGVPFLPPESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180
           GIVTLGVPFLPPES+K SQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS
Sbjct: 121 GIVTLGVPFLPPESLKHSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180

Query: 181 EIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFNEDLGIK 240
           EIP AQENQEVMDLV+PSTPLPPWFTEEDLATYG LYEKSGFDTALK+PYRSFNED GIK
Sbjct: 181 EIPTAQENQEVMDLVEPSTPLPPWFTEEDLATYGTLYEKSGFDTALKVPYRSFNEDWGIK 240

Query: 241 DPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSHFVQEQSPEEV 300
           DPKVEIPALFIMGEKDYV KFPEIEEYVRSERVKDFVP LEI++LPEGSHFVQEQSPEEV
Sbjct: 241 DPKVEIPALFIMGEKDYVFKFPEIEEYVRSERVKDFVPNLEIVYLPEGSHFVQEQSPEEV 300

Query: 301 NQLLLAFLAKHI 313
           N LLL FLAKHI
Sbjct: 301 NHLLLTFLAKHI 312

BLAST of Lsi01G003330 vs. TrEMBL
Match: A0A0A0KK45_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G517170 PE=4 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 1.2e-144
Identity = 246/312 (78.85%), Postives = 278/312 (89.10%), Query Frame = 1

Query: 1   MDRIQHNFIEV-GGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPD 60
           MD+ +H F+EV GGLK+HVAEIGTGSNVVVFLHGFPEIWYSWR+QMIA+A+AGFR +  D
Sbjct: 1   MDKFEHKFVEVKGGLKIHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVANAGFRAIGLD 60

Query: 61  YRGYGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERA 120
           YRGYGLSD PA+PSKA+++DLI+DLL +LD+L+I KVF+V KDFGA PAYYFALKHPERA
Sbjct: 61  YRGYGLSDPPADPSKATYSDLITDLLEVLDSLDISKVFLVGKDFGAMPAYYFALKHPERA 120

Query: 121 LGIVTLGVPFLPPESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSK 180
           LG+VTLGVPF+PP    +   ++PEG Y  RW++PGRAEADF RFDAKTVVRNVYILFS+
Sbjct: 121 LGVVTLGVPFMPPARPINFIDHLPEGFYISRWQKPGRAEADFSRFDAKTVVRNVYILFSR 180

Query: 181 SEIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFNEDLGI 240
           SEIPIAQENQE+MDLVD STPLPPWFTEEDLA YG LYEKSGF TALK+PYRS  ED G+
Sbjct: 181 SEIPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFQTALKVPYRSLGEDWGV 240

Query: 241 KDPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSHFVQEQSPEE 300
           KDPKVEIPAL +MGEKDYVLKFP IEEYVRSE VK +VPKLE+IFLPEGSHFVQEQSPEE
Sbjct: 241 KDPKVEIPALLVMGEKDYVLKFPGIEEYVRSEMVKYYVPKLEVIFLPEGSHFVQEQSPEE 300

Query: 301 VNQLLLAFLAKH 312
           +NQLLL FLAKH
Sbjct: 301 INQLLLNFLAKH 312

BLAST of Lsi01G003330 vs. TrEMBL
Match: M5X1C5_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008997mg PE=4 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 2.4e-137
Identity = 234/312 (75.00%), Postives = 268/312 (85.90%), Query Frame = 1

Query: 1   MDRIQHNFIEVGGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY 60
           M+ IQH +I V GLKLHVA+IGTGSNVVVFLHGFPEIWYSWR+QMIALA AGFR +APDY
Sbjct: 1   MEEIQHKYITVQGLKLHVADIGTGSNVVVFLHGFPEIWYSWRHQMIALARAGFRAIAPDY 60

Query: 61  RGYGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL 120
           RGYGLSDSP +P K S+ DL+SDLLGILD+L IPKVF++AKDFG  PAY FAL HPER L
Sbjct: 61  RGYGLSDSPPQPDKTSYRDLVSDLLGILDSLAIPKVFLIAKDFGPRPAYTFALLHPERVL 120

Query: 121 GIVTLGVPFLPPESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180
           G+VT+GV ++PP   ++ +  +PEG Y  RW+EPGRAEADFGR DAKTVVRNVYILFS+S
Sbjct: 121 GVVTMGVSYVPP-GPRAFEKLLPEGFYISRWQEPGRAEADFGRLDAKTVVRNVYILFSRS 180

Query: 181 EIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFNEDLGIK 240
           EIPIA ENQE+MDLVDPSTPLPPWFTEEDLA YGALYEKSGF TAL++PYR+  ED GI 
Sbjct: 181 EIPIAAENQEIMDLVDPSTPLPPWFTEEDLAAYGALYEKSGFQTALQVPYRALREDPGIT 240

Query: 241 DPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSHFVQEQSPEEV 300
           DP V+ PALFIMG KDYV KFP +E+Y+ S +VK FVPKLEI+FLPEG+HFVQEQSPEEV
Sbjct: 241 DPIVKAPALFIMGGKDYVNKFPGMEDYINSGKVKQFVPKLEIVFLPEGTHFVQEQSPEEV 300

Query: 301 NQLLLAFLAKHI 313
           NQL++ FL KHI
Sbjct: 301 NQLIITFLGKHI 311

BLAST of Lsi01G003330 vs. TrEMBL
Match: A0A059CDK0_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D00644 PE=4 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 2.6e-136
Identity = 229/312 (73.40%), Postives = 267/312 (85.58%), Query Frame = 1

Query: 1   MDRIQHNFIEVGGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY 60
           MD+IQH FIEV GLK HVAEIG+G NVVVFLHGFPEIWYSWR+QM+A+A+AGFR LAPD+
Sbjct: 1   MDQIQHKFIEVQGLKHHVAEIGSGPNVVVFLHGFPEIWYSWRHQMVAVAEAGFRALAPDF 60

Query: 61  RGYGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL 120
           RGYGLSD P EP KASF+DLI DL  +LDAL I KVF+VAKDFGA+PAY F+L H ER  
Sbjct: 61  RGYGLSDPPLEPEKASFSDLIEDLAALLDALGIDKVFLVAKDFGAFPAYLFSLLHQERVQ 120

Query: 121 GIVTLGVPFLPPESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180
           GI+TLG+P+ PP        ++PEG Y  RW+EPGRAEADFGR DAKTVVRN+YILFS+S
Sbjct: 121 GIMTLGIPYRPPNRPVKYNEHLPEGFYISRWQEPGRAEADFGRLDAKTVVRNIYILFSRS 180

Query: 181 EIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFNEDLGIK 240
           EIPIA ENQE+MD+VDPSTPLPPWFTE DL  YG+LY KSGF TAL++PYRSF EDLGI 
Sbjct: 181 EIPIAAENQEIMDIVDPSTPLPPWFTEGDLEEYGSLYRKSGFRTALQVPYRSFKEDLGIT 240

Query: 241 DPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSHFVQEQSPEEV 300
           D  V++PAL +MGEKDYVLKFP IEEY+RS +VK+FVP+LEI+FLPEG+HF+QEQSP+EV
Sbjct: 241 DVIVKVPALLVMGEKDYVLKFPGIEEYIRSGKVKEFVPQLEIVFLPEGTHFIQEQSPDEV 300

Query: 301 NQLLLAFLAKHI 313
           NQL+L+FL KHI
Sbjct: 301 NQLILSFLKKHI 312

BLAST of Lsi01G003330 vs. TrEMBL
Match: A0A061GGM4_THECC (Alpha/beta-Hydrolases superfamily protein OS=Theobroma cacao GN=TCM_029913 PE=4 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 2.4e-134
Identity = 226/312 (72.44%), Postives = 263/312 (84.29%), Query Frame = 1

Query: 1   MDRIQHNFIEVGGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY 60
           M++IQHNF+ V GLK HVAEIGTGSNVVVFLHGFPEIWY+WR+QM+A+ADAGFR +APDY
Sbjct: 1   MEQIQHNFVTVRGLKFHVAEIGTGSNVVVFLHGFPEIWYTWRHQMVAVADAGFRAIAPDY 60

Query: 61  RGYGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL 120
           RGYGLSD P EP + SF D++SDL+ ILD L + KVF+V KDFG WPAY+ AL HP+R  
Sbjct: 61  RGYGLSDIPPEPERTSFADIVSDLVAILDHLGVNKVFLVGKDFGVWPAYHVALLHPDRVS 120

Query: 121 GIVTLGVPFLPPESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180
            ++TLGVP+LPPE  K  QS +PEG Y  RWREPGRAEADFGRFDAKTVVRN+YILFS+S
Sbjct: 121 AVITLGVPYLPPEPPKFHQS-LPEGFYISRWREPGRAEADFGRFDAKTVVRNIYILFSRS 180

Query: 181 EIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFNEDLGIK 240
           EIPIA ENQE+MDLVD STPLPPWFTEEDLA YGALYEKSGF TAL++PYRSF ED GI 
Sbjct: 181 EIPIAAENQEIMDLVDASTPLPPWFTEEDLAAYGALYEKSGFRTALQVPYRSFEEDFGIT 240

Query: 241 DPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSHFVQEQSPEEV 300
           DP V++PAL IMG KDYV KFP IEEY++  + ++ VP L+II+LPEG+HFVQEQSPE V
Sbjct: 241 DPIVKVPALLIMGCKDYVFKFPGIEEYIKFGKAQELVPGLDIIYLPEGTHFVQEQSPELV 300

Query: 301 NQLLLAFLAKHI 313
           N+L+L FL  HI
Sbjct: 301 NELILDFLKSHI 311

BLAST of Lsi01G003330 vs. TAIR10
Match: AT3G51000.1 (AT3G51000.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 208.0 bits (528), Expect = 8.5e-54
Identity = 112/316 (35.44%), Postives = 186/316 (58.86%), Query Frame = 1

Query: 4   IQHNFIEVGGLKLHVAEIGTGSN-VVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDYRG 63
           ++   I+  G+ L+VAE G     +V+ LHGFPE WYSWR+Q+  L+  G+ V+APD RG
Sbjct: 5   VREKKIKTNGIWLNVAEKGDEEGPLVLLLHGFPETWYSWRHQIDFLSSHGYHVVAPDLRG 64

Query: 64  YGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERALGI 123
           YG SDS       + + L++D++G+LD     + FV   D+GA   +   L  P+R  G 
Sbjct: 65  YGDSDSLPSHESYTVSHLVADVIGLLDHYGTTQAFVAGHDWGAIIGWCLCLFRPDRVKGF 124

Query: 124 VTLGVPFLPPE-SVKSSQ--SNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSK 183
           ++L VP+ P +  +K S       +G+Y  ++++PGRAEA F + D  +V++  ++L ++
Sbjct: 125 ISLSVPYFPRDPKLKPSDFFKIFGDGLYITQFQKPGRAEAAFAKHDCLSVMKK-FLLITR 184

Query: 184 SEIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFNEDLGI 243
           ++  +A  + E++D ++  + +P W TEE++  Y   +++SGF   L   YRS + +  I
Sbjct: 185 TDYLVAPPDTEIIDHLEIPSTIPDWITEEEIQVYAEKFQRSGFTGPLNY-YRSMDMNWEI 244

Query: 244 ----KDPKVEIPALFIMGEKDYVLKFPE-IEEYVRSERVKDFVPKLEIIFLPEGSHFVQE 303
               +D K+ +P  FI G+KD   + P    EYV+ E  K  VP LEI+ +  G HF+Q+
Sbjct: 245 LAPWQDSKIVVPTKFIAGDKDIGYEGPNGTMEYVKGEVFKIVVPNLEIVVIEGGHHFIQQ 304

Query: 304 QSPEEVNQLLLAFLAK 311
           +  E+V+Q +L+FL K
Sbjct: 305 EKSEQVSQEILSFLNK 318

BLAST of Lsi01G003330 vs. TAIR10
Match: AT4G02340.1 (AT4G02340.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 206.5 bits (524), Expect = 2.5e-53
Identity = 106/319 (33.23%), Postives = 176/319 (55.17%), Query Frame = 1

Query: 1   MDRIQHNFIEVGGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY 60
           M++I+H  I   G+ +HVA IG+G  V++F+HGFP++WYSWR+Q+++ A  G+R +APD 
Sbjct: 1   MEKIEHTTISTNGINMHVASIGSGP-VILFVHGFPDLWYSWRHQLVSFAALGYRAIAPDL 60

Query: 61  RGYGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL 120
           RGYG SD+P      +   ++ DL+G+LD+L + +VF+V  D+GA  A++  +  P+R  
Sbjct: 61  RGYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWLCMIRPDRVN 120

Query: 121 GIVTLGVPFLP----PESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYIL 180
            +V   V F P     + V + ++   +  Y  R++EPG  E DF + D K ++   +  
Sbjct: 121 ALVNTSVVFNPRNPSVKPVDAFRALFGDDYYICRFQEPGEIEEDFAQVDTKKLITR-FFT 180

Query: 181 FSKSEIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFNED 240
                 P   ++     L DP + LP W TE+D+  YG  + + GF   L   YR+ N  
Sbjct: 181 SRNPRPPCIPKSVGFRGLPDPPS-LPAWLTEQDVRFYGDKFSQKGFTGGLNY-YRALNLS 240

Query: 241 LGIKDP----KVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEG-SHF 300
             +  P    ++++P  FI+G+ D     P  +EY+    +K  VP L+ + + EG  HF
Sbjct: 241 WELTAPWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVGHF 300

Query: 301 VQEQSPEEVNQLLLAFLAK 311
           + ++ P+EV   +  F  K
Sbjct: 301 LHQEKPDEVTDHIYGFFKK 315

BLAST of Lsi01G003330 vs. TAIR10
Match: AT2G26740.1 (AT2G26740.1 soluble epoxide hydrolase)

HSP 1 Score: 198.7 bits (504), Expect = 5.1e-51
Identity = 109/322 (33.85%), Postives = 180/322 (55.90%), Query Frame = 1

Query: 4   IQHNFIEVGGLKLHVAEIGTGSN-VVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDYRG 63
           ++H  +   G+ +HVA  G     +V+ LHGFPE+WYSWR+Q+  LA  G+R +APD RG
Sbjct: 1   MEHRKVRGNGIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAVAPDLRG 60

Query: 64  YGLSDSPAEPSKASFNDLISDLLGILDALNI---PKVFVVAKDFGAWPAYYFALKHPERA 123
           YG SD+PAE S  +  +++ DL+ ++ AL      KVFVV  D+GA  A+Y  L  P+R 
Sbjct: 61  YGDSDAPAEISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAWYLCLFRPDRV 120

Query: 124 LGIVTLGVPFL------PPESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNV 183
             +V L VPF         + V   ++   +  Y  R++E G  EA+      + V++ +
Sbjct: 121 KALVNLSVPFSFRPTDPSVKPVDRMRAFYGDDYYICRFQEFGDVEAEIAEVGTERVMKRL 180

Query: 184 YILFSKSEIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSF 243
               +   + I  +++        + PLP W TEED+A + + +E+ GF   +   YR+F
Sbjct: 181 LTYRTPGPV-IIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFEEKGFSGPVNY-YRNF 240

Query: 244 NEDLGIKDP----KVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEG- 303
           N +  +  P    K+++P  F++GE D V   P ++EY+   + K+ VP LE   + EG 
Sbjct: 241 NRNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLLEEPVVMEGV 300

Query: 304 SHFVQEQSPEEVNQLLLAFLAK 311
           +HF+ ++ P+E+ Q++L F++K
Sbjct: 301 AHFINQEKPQEILQIILDFISK 320

BLAST of Lsi01G003330 vs. TAIR10
Match: AT2G26750.1 (AT2G26750.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 189.1 bits (479), Expect = 4.1e-48
Identity = 105/320 (32.81%), Postives = 179/320 (55.94%), Query Frame = 1

Query: 4   IQHNFIEVGGLKLHVAEIG-TGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDYRG 63
           ++H  +   G+ +HVA  G +   +V+ LHGFPE+WYSWR+Q+  LA  G+R +APD RG
Sbjct: 1   MEHRNVRGNGIDIHVAIQGPSDGTIVLLLHGFPELWYSWRHQISGLAARGYRAVAPDLRG 60

Query: 64  YGLSDSPAEPSKASFNDLISDLLGILDAL--NIPKVFVVAKDFGAWPAYYFALKHPERAL 123
           YG SD+PAE S  +  +++ DL+ ++  L     KVFVV  D+GA  A+Y  L  P++  
Sbjct: 61  YGDSDAPAEISSFTCFNIVGDLVAVISTLIKEDKKVFVVGHDWGALIAWYLCLFRPDKVK 120

Query: 124 GIVTLGVP--FLPPE----SVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVY 183
            +V L VP  F P +     V   ++      Y  R++E G  EA+      + V++ + 
Sbjct: 121 ALVNLSVPLSFWPTDPSVKPVDRMRAVYGNDYYVCRFQEVGDIEAEIAEVGTERVMKRLL 180

Query: 184 ILFSKSEIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFN 243
              +   + I  +++        + PLP W TEED+A + + +++ GF   +   YR+FN
Sbjct: 181 TYRTPGPL-IIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFKEKGFCGPVNY-YRNFN 240

Query: 244 EDLGIKDP----KVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEG-S 303
            +  +  P    K+++P  F++GE D V   P ++EY+   + K+ VP +E   + EG +
Sbjct: 241 RNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLIEEPVVMEGVA 300

Query: 304 HFVQEQSPEEVNQLLLAFLA 310
           HF+ ++ P+E+ Q++L F++
Sbjct: 301 HFLNQEKPQEILQIILDFIS 318

BLAST of Lsi01G003330 vs. TAIR10
Match: AT3G05600.1 (AT3G05600.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 187.6 bits (475), Expect = 1.2e-47
Identity = 108/327 (33.03%), Postives = 181/327 (55.35%), Query Frame = 1

Query: 1   MDRIQHNFIEVGGLKLHVAEIGTGSN-VVVFLHGFPEIWYSWRYQMIALADAGFRVLAPD 60
           M+ I H  + V G+ +H+AE G     VV+ LHGFP++WY+WR+Q+  L+  G+R +APD
Sbjct: 1   MEGIDHRMVSVNGITMHIAEKGPKEGPVVLLLHGFPDLWYTWRHQISGLSSLGYRAVAPD 60

Query: 61  YRGYGLSDSPAEPSKASFNDLISDLLGILDAL--NIPKVFVVAKDFGAWPAYYFALKHPE 120
            RGYG SDSP   S+ +  +++ DL+ +LD++  N  KVF+V  D+GA   ++  L  PE
Sbjct: 61  LRGYGDSDSPESFSEYTCLNVVGDLVALLDSVAGNQEKVFLVGHDWGAIIGWFLCLFRPE 120

Query: 121 RALGIVTLGVPFL----PPESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNV 180
           +  G V L VP+       + V+  ++   +  Y  R++EPG+ E +    D +  +RN 
Sbjct: 121 KINGFVCLSVPYRSRNPKVKPVQGFKAVFGDDYYICRFQEPGKIEGEIASADPRIFLRN- 180

Query: 181 YILFSKSEI--PIAQENQEVMDLVDPST---PLPPWFTEEDLATYGALYEKSGFDTALKI 240
             LF+   +  PI  ++    +  +P++    LP WF+++DL  Y + +EK+GF   L  
Sbjct: 181 --LFTGRTLGPPILPKDNPFGEKPNPNSENIELPEWFSKKDLDFYVSKFEKAGFTGGLNY 240

Query: 241 PYRSFNEDLGIKDP----KVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKL-EII 300
            YR+ + +  +  P    K+++P  F+ G+ D V   P ++EY+        VP L EI+
Sbjct: 241 -YRAMDLNWELTAPWTGAKIQVPVKFMTGDFDMVYTTPGMKEYIHGGGFAADVPTLQEIV 300

Query: 301 FLPEGSHFVQEQSPEEVNQLLLAFLAK 311
            + +  HFV ++ P+EV   +  F  K
Sbjct: 301 VIEDAGHFVNQEKPQEVTAHINDFFTK 323

BLAST of Lsi01G003330 vs. NCBI nr
Match: gi|449433871|ref|XP_004134720.1| (PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis sativus])

HSP 1 Score: 614.0 bits (1582), Expect = 1.5e-172
Identity = 296/312 (94.87%), Postives = 302/312 (96.79%), Query Frame = 1

Query: 1   MDRIQHNFIEVGGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY 60
           MDRI HNFIEVG LKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY
Sbjct: 1   MDRIHHNFIEVGALKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY 60

Query: 61  RGYGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL 120
           RGYGLSDSPAEPSKASF+DLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL
Sbjct: 61  RGYGLSDSPAEPSKASFSDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL 120

Query: 121 GIVTLGVPFLPPESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180
           GIVTLGVPFLPPES+K SQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS
Sbjct: 121 GIVTLGVPFLPPESLKHSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180

Query: 181 EIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFNEDLGIK 240
           EIP AQENQEVMDLV+PSTPLPPWFTEEDLATYG LYEKSGFDTALK+PYRSFNED GIK
Sbjct: 181 EIPTAQENQEVMDLVEPSTPLPPWFTEEDLATYGTLYEKSGFDTALKVPYRSFNEDWGIK 240

Query: 241 DPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSHFVQEQSPEEV 300
           DPKVEIPALFIMGEKDYV KFPEIEEYVRSERVKDFVP LEI++LPEGSHFVQEQSPEEV
Sbjct: 241 DPKVEIPALFIMGEKDYVFKFPEIEEYVRSERVKDFVPNLEIVYLPEGSHFVQEQSPEEV 300

Query: 301 NQLLLAFLAKHI 313
           N LLL FLAKHI
Sbjct: 301 NHLLLTFLAKHI 312

BLAST of Lsi01G003330 vs. NCBI nr
Match: gi|659078745|ref|XP_008439884.1| (PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo])

HSP 1 Score: 610.9 bits (1574), Expect = 1.2e-171
Identity = 293/312 (93.91%), Postives = 303/312 (97.12%), Query Frame = 1

Query: 1   MDRIQHNFIEVGGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY 60
           MDRI HNFI+VG LKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY
Sbjct: 63  MDRIHHNFIQVGALKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY 122

Query: 61  RGYGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL 120
           RGYGLSDSPAEPSKASF+DLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL
Sbjct: 123 RGYGLSDSPAEPSKASFSDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL 182

Query: 121 GIVTLGVPFLPPESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180
           GIVTLGVPFLPPES+K SQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS
Sbjct: 183 GIVTLGVPFLPPESLKHSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 242

Query: 181 EIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFNEDLGIK 240
           EIP AQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALK+PYRSFNED GIK
Sbjct: 243 EIPTAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKVPYRSFNEDWGIK 302

Query: 241 DPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSHFVQEQSPEEV 300
           DP+VEIPALFIMGEKDYV KFPE++EYVRSERVKDFVP LEI++LPEGSHFVQEQSPEEV
Sbjct: 303 DPRVEIPALFIMGEKDYVFKFPEVDEYVRSERVKDFVPNLEIVYLPEGSHFVQEQSPEEV 362

Query: 301 NQLLLAFLAKHI 313
           N LLL FLAK+I
Sbjct: 363 NHLLLTFLAKYI 374

BLAST of Lsi01G003330 vs. NCBI nr
Match: gi|449433873|ref|XP_004134721.1| (PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis sativus])

HSP 1 Score: 520.8 bits (1340), Expect = 1.7e-144
Identity = 246/312 (78.85%), Postives = 278/312 (89.10%), Query Frame = 1

Query: 1   MDRIQHNFIEV-GGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPD 60
           MD+ +H F+EV GGLK+HVAEIGTGSNVVVFLHGFPEIWYSWR+QMIA+A+AGFR +  D
Sbjct: 1   MDKFEHKFVEVKGGLKIHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVANAGFRAIGLD 60

Query: 61  YRGYGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERA 120
           YRGYGLSD PA+PSKA+++DLI+DLL +LD+L+I KVF+V KDFGA PAYYFALKHPERA
Sbjct: 61  YRGYGLSDPPADPSKATYSDLITDLLEVLDSLDISKVFLVGKDFGAMPAYYFALKHPERA 120

Query: 121 LGIVTLGVPFLPPESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSK 180
           LG+VTLGVPF+PP    +   ++PEG Y  RW++PGRAEADF RFDAKTVVRNVYILFS+
Sbjct: 121 LGVVTLGVPFMPPARPINFIDHLPEGFYISRWQKPGRAEADFSRFDAKTVVRNVYILFSR 180

Query: 181 SEIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFNEDLGI 240
           SEIPIAQENQE+MDLVD STPLPPWFTEEDLA YG LYEKSGF TALK+PYRS  ED G+
Sbjct: 181 SEIPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFQTALKVPYRSLGEDWGV 240

Query: 241 KDPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSHFVQEQSPEE 300
           KDPKVEIPAL +MGEKDYVLKFP IEEYVRSE VK +VPKLE+IFLPEGSHFVQEQSPEE
Sbjct: 241 KDPKVEIPALLVMGEKDYVLKFPGIEEYVRSEMVKYYVPKLEVIFLPEGSHFVQEQSPEE 300

Query: 301 VNQLLLAFLAKH 312
           +NQLLL FLAKH
Sbjct: 301 INQLLLNFLAKH 312

BLAST of Lsi01G003330 vs. NCBI nr
Match: gi|659078749|ref|XP_008439886.1| (PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo])

HSP 1 Score: 517.7 bits (1332), Expect = 1.4e-143
Identity = 245/312 (78.53%), Postives = 279/312 (89.42%), Query Frame = 1

Query: 1   MDRIQHNFIEV-GGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPD 60
           MD+ +H F+EV GGLK+HVAEIGTGSNVVVFLHGFPEIWYSWR+QMIA+A+AGFR +  D
Sbjct: 1   MDKFEHKFVEVKGGLKIHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVANAGFRAIGLD 60

Query: 61  YRGYGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERA 120
           YRGYGLSD PA+PSKA+++DLI+DLL +LD+L+I KVF+V KDFGA PAYYFALKHPERA
Sbjct: 61  YRGYGLSDPPADPSKATYSDLITDLLEVLDSLDISKVFLVGKDFGAMPAYYFALKHPERA 120

Query: 121 LGIVTLGVPFLPPESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSK 180
           LG+VTLGVPF+PP    +    +PEG Y  RW++PGRAEADF RFDAKTVVRNVYILFS+
Sbjct: 121 LGVVTLGVPFMPPGRRINFIDYLPEGFYISRWQKPGRAEADFSRFDAKTVVRNVYILFSR 180

Query: 181 SEIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFNEDLGI 240
           SEIPIAQENQE+MDLVD STPLPPWFTEEDLA YG LYEKSGF TAL++PYRS +ED G+
Sbjct: 181 SEIPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFQTALQVPYRSLSEDWGV 240

Query: 241 KDPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSHFVQEQSPEE 300
           +DPKVEIPALF+MGEKDYVLKFP +EEYVRSE VK +VPKLE+IFLPEGSHFVQEQSPEE
Sbjct: 241 EDPKVEIPALFVMGEKDYVLKFPGMEEYVRSEMVKYYVPKLEVIFLPEGSHFVQEQSPEE 300

Query: 301 VNQLLLAFLAKH 312
           VNQLLL FLAKH
Sbjct: 301 VNQLLLNFLAKH 312

BLAST of Lsi01G003330 vs. NCBI nr
Match: gi|595863839|ref|XP_007211661.1| (hypothetical protein PRUPE_ppa008997mg [Prunus persica])

HSP 1 Score: 496.5 bits (1277), Expect = 3.4e-137
Identity = 234/312 (75.00%), Postives = 268/312 (85.90%), Query Frame = 1

Query: 1   MDRIQHNFIEVGGLKLHVAEIGTGSNVVVFLHGFPEIWYSWRYQMIALADAGFRVLAPDY 60
           M+ IQH +I V GLKLHVA+IGTGSNVVVFLHGFPEIWYSWR+QMIALA AGFR +APDY
Sbjct: 1   MEEIQHKYITVQGLKLHVADIGTGSNVVVFLHGFPEIWYSWRHQMIALARAGFRAIAPDY 60

Query: 61  RGYGLSDSPAEPSKASFNDLISDLLGILDALNIPKVFVVAKDFGAWPAYYFALKHPERAL 120
           RGYGLSDSP +P K S+ DL+SDLLGILD+L IPKVF++AKDFG  PAY FAL HPER L
Sbjct: 61  RGYGLSDSPPQPDKTSYRDLVSDLLGILDSLAIPKVFLIAKDFGPRPAYTFALLHPERVL 120

Query: 121 GIVTLGVPFLPPESVKSSQSNIPEGVYTLRWREPGRAEADFGRFDAKTVVRNVYILFSKS 180
           G+VT+GV ++PP   ++ +  +PEG Y  RW+EPGRAEADFGR DAKTVVRNVYILFS+S
Sbjct: 121 GVVTMGVSYVPP-GPRAFEKLLPEGFYISRWQEPGRAEADFGRLDAKTVVRNVYILFSRS 180

Query: 181 EIPIAQENQEVMDLVDPSTPLPPWFTEEDLATYGALYEKSGFDTALKIPYRSFNEDLGIK 240
           EIPIA ENQE+MDLVDPSTPLPPWFTEEDLA YGALYEKSGF TAL++PYR+  ED GI 
Sbjct: 181 EIPIAAENQEIMDLVDPSTPLPPWFTEEDLAAYGALYEKSGFQTALQVPYRALREDPGIT 240

Query: 241 DPKVEIPALFIMGEKDYVLKFPEIEEYVRSERVKDFVPKLEIIFLPEGSHFVQEQSPEEV 300
           DP V+ PALFIMG KDYV KFP +E+Y+ S +VK FVPKLEI+FLPEG+HFVQEQSPEEV
Sbjct: 241 DPIVKAPALFIMGGKDYVNKFPGMEDYINSGKVKQFVPKLEIVFLPEGTHFVQEQSPEEV 300

Query: 301 NQLLLAFLAKHI 313
           NQL++ FL KHI
Sbjct: 301 NQLIITFLGKHI 311

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
HYES_HUMAN6.1e-3833.33Bifunctional epoxide hydrolase 2 OS=Homo sapiens GN=EPHX2 PE=1 SV=2[more]
HYES_RAT6.1e-3833.23Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus GN=Ephx2 PE=1 SV=1[more]
HYES_MOUSE8.0e-3831.97Bifunctional epoxide hydrolase 2 OS=Mus musculus GN=Ephx2 PE=1 SV=2[more]
HYES_PIG1.8e-3432.38Bifunctional epoxide hydrolase 2 OS=Sus scrofa GN=EPHX2 PE=2 SV=1[more]
YFHM_BACSU1.1e-3128.75AB hydrolase superfamily protein YfhM OS=Bacillus subtilis (strain 168) GN=yfhM ... [more]
Match NameE-valueIdentityDescription
A0A0A0KHK9_CUCSA1.0e-17294.87Uncharacterized protein OS=Cucumis sativus GN=Csa_6G517180 PE=4 SV=1[more]
A0A0A0KK45_CUCSA1.2e-14478.85Uncharacterized protein OS=Cucumis sativus GN=Csa_6G517170 PE=4 SV=1[more]
M5X1C5_PRUPE2.4e-13775.00Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008997mg PE=4 SV=1[more]
A0A059CDK0_EUCGR2.6e-13673.40Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D00644 PE=4 SV=1[more]
A0A061GGM4_THECC2.4e-13472.44Alpha/beta-Hydrolases superfamily protein OS=Theobroma cacao GN=TCM_029913 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT3G51000.18.5e-5435.44 alpha/beta-Hydrolases superfamily protein[more]
AT4G02340.12.5e-5333.23 alpha/beta-Hydrolases superfamily protein[more]
AT2G26740.15.1e-5133.85 soluble epoxide hydrolase[more]
AT2G26750.14.1e-4832.81 alpha/beta-Hydrolases superfamily protein[more]
AT3G05600.11.2e-4733.03 alpha/beta-Hydrolases superfamily protein[more]
Match NameE-valueIdentityDescription
gi|449433871|ref|XP_004134720.1|1.5e-17294.87PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis sativus][more]
gi|659078745|ref|XP_008439884.1|1.2e-17193.91PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo][more]
gi|449433873|ref|XP_004134721.1|1.7e-14478.85PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis sativus][more]
gi|659078749|ref|XP_008439886.1|1.4e-14378.53PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo][more]
gi|595863839|ref|XP_007211661.1|3.4e-13775.00hypothetical protein PRUPE_ppa008997mg [Prunus persica][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
Vocabulary: INTERPRO
TermDefinition
IPR000639Epox_hydrolase-like
IPR000073AB_hydrolase_1
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0008152 metabolic process
biological_process GO:0009636 response to toxic substance
biological_process GO:0006805 xenobiotic metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0016740 transferase activity
molecular_function GO:0004301 epoxide hydrolase activity
molecular_function GO:0003824 catalytic activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi01G003330.1Lsi01G003330.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 52..67
score: 6.2E-5coord: 112..125
score: 6.
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 26..284
score: 3.7
IPR000639Epoxide hydrolase-likePRINTSPR00412EPOXHYDRLASEcoord: 98..111
score: 6.9E-29coord: 112..125
score: 6.9E-29coord: 285..307
score: 6.9E-29coord: 32..50
score: 6.9E-29coord: 52..67
score: 6.9E-29coord: 246..262
score: 6.9
NoneNo IPR availablePANTHERPTHR10992ALPHA/BETA HYDROLASE FOLD-CONTAINING PROTEINcoord: 1..162
score: 1.2E-153coord: 199..310
score: 1.2E
NoneNo IPR availablePANTHERPTHR10992:SF869SUBFAMILY NOT NAMEDcoord: 1..162
score: 1.2E-153coord: 199..310
score: 1.2E

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Lsi01G003330Lsi02G007370Bottle gourd (USVL1VR-Ls)lsilsiB066