Cucsa.397230 (gene) Cucumber (Gy14) v1

NameCucsa.397230
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionEffector of transcription protein
Locationscaffold04146 : 57674 .. 60761 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTTTAATCATGTACAACAACCTCAGATCCAGCCTTCGCTTCAAATCGGAGCAATTCTTCTCTCTTTCTTAACTGCCGCTCACTATGGTCGCCGAGCTTTCTCCCATCAGGCTGAAGAGGGAAGACTGTAAACGCACCAAACACGACTCCGATTTCTCCAAATGGGAGGTTTGATCTTTTTCTCTATTTGTTTCTATCTTCAAATTTTGGTGGGTTCCCTTCTTGGTTACAGAAATGAGCTTAAATTTGAAGCTACCGCTTTCTTTTAGGGTAATAATTATGTATCAGCTTCGGTTGGTGTTTTCGTTTGACCATCCAAGACTCTTGCTTTCCATTTGTGTTTTCACTAGTGATACCCAAGCAAAGTTTCACATCCGCGCTAGATAAATAGATGATCATAAGTCGTTCTGAGTGAAACTAAAGTCACCATGGAGATATGCGAAGATTCGTTGGGACAACGAGTGACTTGCTTGTTATTAGTAGTAACCGAAGTGGTGCCTCTTTGGGGAGCCTCGGATTGATGTTCCTTGGATTGTTGGTTTTTGTTTGGATTTGCTCTTTTGTTTCACAAGATATTTGAGGGCTTCTCACATCCTCAATCTAGCTTGCATCTTTCTGTTTCTCTCTTTTTTTGTCTTCTCCTTCCACCTTGTTTTTTGTTGTAACTATTTAATCCGCGGTTGTCCCTCTGGTTCTTAATACATCTCTTTATGGATTTTTACTGCTATACTCTCTTGATATTTCATATATCAATGAAATTGTTTCTTATTCAAAAAAAAAAAAAACGCCAAAGCAGTGCCTCGTGTAGGCATCATAACCAAACTTAAGGAACATGTTGATGGGATTTCTTGGGGCTGTTTACTCTTTTCCATATTCCTCAATGCGTTGCTTGTTGTGATGCCTCAGATTCTTGTTGCCTCTTCTGATTGGGAAGACTATTCATTGGGGAAGGAAGGTGCAGAAAGGTACAGAATTCACAATCTTCCTAAAGTTTCAGGTCCTGGATTGTATGAGCTTGGCATTACTGTATCTAGCTCCGGTTTAGGTCGTGAGATAGCTAAACTAGACGCAGACTGGATAGTTGTTGTCTACCTTGGCGAAGCTGACAACGTGAGAACACGATTACAGCACTATGGTCGCTCGGGTTCTCATTTGGGCAATGCCTATTTCTGTGTTGTTGACTGTAAGGTTTTTCCTCTTGAAAAGGGACCCAGTTTATTTCAAGAAATGTTCTCAAGAGGGTACTCCATTGTGTATAGATGGGCTCCTGTAAGTATGTTTTGTTTTTATATATACTTTAATCAAATAATTTCATTTGGTTCTTGTAGTGCCTTGGGGAAGTAGCTAATCTACTGGATTGGTTTGTCTCATGATAGCAATTTTTTATTGGCATATAAATGTTGGTTGTTTGCCGTATCATTTGAGTTTTTTGAGATGAAAGCTGTACTTTATATATTTAAGGGATGCGAAAACAGTTCTTGCAAATGCATGAATTGTTAATCCCTCATGGGGCAGTCCTATTGGTTAAGGATTATGGTCTCTAAGTCTGAGAAATGCATGCCTAGAGGCTGCGAGTAATCTTAATAAGGAAAGTCTTTGATGTCTCGTAGTTTTGGTCTCAGGACAGGCAAAAAAAAAAAGGTGTACATGAATTGTTATGTTAATATTAAAGATTTCATCATCTCCTCTCAAGGTCAATTATGATTGTATCTCTTTCTGCATTTTGTTGTTTTTACCATTCCATGTTTCTCATTTTTCTTGTTGCTTTGACAGATGAAAAACAAGAAGAATGCTCAGATGACTGAAACTCAACTGCTTAAAACATTTGACTATGCATGGAATACAAGTGGTAATGGTGCACGGCGCCATGACGATGTACTAAAAAAACTTGAGAACATCGCTTCACAAACTACAAAATCTACCTTTATTTCCAGAAAGCTTCTGCCTTTTACACAGAAGAAAATGGGCATCAAAATTAAAACAAGCAAGTCAATTCCAATGGTGAACAAACCAGCTGAAGATGCTGAGGAGAGGAACAATTTCTTTTCTCGCATTCTCAGTTTCAGCAGATCTCGGCCTAGACTTGTTGACAACACTAATGATGTTAATTGGGCAGATAGCAATAGCTGCGGAGTGGTCATAGGTCATAACGGTGAGGTTTGTAGGAAGCCCCCAGTTGAAGGAAGAAAAAGGTGTGCTGAACATAAAGGGATGAAGATTAATGGATTACTAAAGAACTCATCTAGCAGATTTATTTTACAAAAGCCCGTGAATGTAGGAACAACCATATATGGTGAAAAAGACTTCTCTTGTAGTAAATCTGAGATTCCCAATAGCACAGAAGAATGCTCAGTTTCGAATTCATTTCCCAATAAGGGGAGTTCTCTTCCAATTTGTGGAGTTGTTCTGTATGATGGCTCTCCATGTAGAAGACCCCCAGTTCAAGGAAGGAAACGGTGTGAAGAACACAAAGGGCAACGAATTTGTAGATCGACTTTAGTCACTTCCAAGTACCAGCAAACTTCACCCATATCAAAACCTGAGTCAACTGTGATAGCACAAGGCACTTCTTCAGAACTTTCTAGCTCAGGTCTCGAAAGAATGTGTGGTGTTGATTTAGGCAATGGACTCAACTGCACTAGGCAGCCAGTGAAAGGACGAGTTAGGTGTGGGGAACATAAAGGAATGAGAACTAATAAGTTGATTTCAACGTTAGCCACAAGCAACAAACCGGATGTGTCCGATACCGGTTCCGTCAGCTAAATTTTTAATCCAGCTAAAAGTTTGTGATAATATCCGTAAACCAACTTGGCAATTCTCCTTCCTTATTCATCAAGAGGAAATTCATAGCGTCACAATTCAGAATAAAACCATCTGTTACCAGGACTCCGCTGGCTACATGGTGAACAACTTCAGCAAACAGGTCAAACTTGTGTAACTTAGGGTACATGTTTTTGTTGTATGATATGTACTTATTCTAATTTATCATTGTATCTTGAACTTATAGTTGCTTGTTGCCTTGATGAACACAGAAGAGATAAGTCTCCTTACAAAATTTGAGTCTACTTTCTTTTTTCCAACAAGAGC

mRNA sequence

GTTTTTAATCATGTACAACAACCTCAGATCCAGCCTTCGCTTCAAATCGGAGCAATTCTTCTCTCTTTCTTAACTGCCGCTCACTATGGTCGCCGAGCTTTCTCCCATCAGGCTGAAGAGGGAAGACTGTAAACGCACCAAACACGACTCCGATTTCTCCAAATGGGAGATTCTTGTTGCCTCTTCTGATTGGGAAGACTATTCATTGGGGAAGGAAGGTGCAGAAAGGTACAGAATTCACAATCTTCCTAAAGTTTCAGGTCCTGGATTGTATGAGCTTGGCATTACTGTATCTAGCTCCGGTTTAGGTCGTGAGATAGCTAAACTAGACGCAGACTGGATAGTTGTTGTCTACCTTGGCGAAGCTGACAACGTGAGAACACGATTACAGCACTATGGTCGCTCGGGTTCTCATTTGGGCAATGCCTATTTCTGTGTTGTTGACTGTAAGGTTTTTCCTCTTGAAAAGGGACCCAGTTTATTTCAAGAAATGTTCTCAAGAGGGTACTCCATTGTGTATAGATGGGCTCCTATGAAAAACAAGAAGAATGCTCAGATGACTGAAACTCAACTGCTTAAAACATTTGACTATGCATGGAATACAAGTGGTAATGGTGCACGGCGCCATGACGATGTACTAAAAAAACTTGAGAACATCGCTTCACAAACTACAAAATCTACCTTTATTTCCAGAAAGCTTCTGCCTTTTACACAGAAGAAAATGGGCATCAAAATTAAAACAAGCAAGTCAATTCCAATGGTGAACAAACCAGCTGAAGATGCTGAGGAGAGGAACAATTTCTTTTCTCGCATTCTCAGTTTCAGCAGATCTCGGCCTAGACTTGTTGACAACACTAATGATGTTAATTGGGCAGATAGCAATAGCTGCGGAGTGGTCATAGGTCATAACGGTGAGGTTTGTAGGAAGCCCCCAGTTGAAGGAAGAAAAAGGTGTGCTGAACATAAAGGGATGAAGATTAATGGATTACTAAAGAACTCATCTAGCAGATTTATTTTACAAAAGCCCGTGAATGTAGGAACAACCATATATGGTGAAAAAGACTTCTCTTGTAGTAAATCTGAGATTCCCAATAGCACAGAAGAATGCTCAGTTTCGAATTCATTTCCCAATAAGGGGAGTTCTCTTCCAATTTGTGGAGTTGTTCTGTATGATGGCTCTCCATGTAGAAGACCCCCAGTTCAAGGAAGGAAACGGTGTGAAGAACACAAAGGGCAACGAATTTGTAGATCGACTTTAGTCACTTCCAAGTACCAGCAAACTTCACCCATATCAAAACCTGAGTCAACTGTGATAGCACAAGGCACTTCTTCAGAACTTTCTAGCTCAGGTCTCGAAAGAATGTGTGGTGTTGATTTAGGCAATGGACTCAACTGCACTAGGCAGCCAGTGAAAGGACGAGTTAGGTGTGGGGAACATAAAGGAATGAGAACTAATAAGTTGATTTCAACGTTAGCCACAAGCAACAAACCGGATGTGTCCGATACCGGTTCCGTCAGCTAAATTTTTAATCCAGCTAAAAGTTTGTGATAATATCCGTAAACCAACTTGGCAATTCTCCTTCCTTATTCATCAAGAGGAAATTCATAGCGTCACAATTCAGAATAAAACCATCTGTTACCAGGACTCCGCTGGCTACATGGTGAACAACTTCAGCAAACAGGTCAAACTTGTGTAACTTAGGGTACATGTTTTTGTTGTATGATATGTACTTATTCTAATTTATCATTGTATCTTGAACTTATAGTTGCTTGTTGCCTTGATGAACACAGAAGAGATAAGTCTCCTTACAAAATTTGAGTCTACTTTCTTTTTTCCAACAAGAGC

Coding sequence (CDS)

ATGGTCGCCGAGCTTTCTCCCATCAGGCTGAAGAGGGAAGACTGTAAACGCACCAAACACGACTCCGATTTCTCCAAATGGGAGATTCTTGTTGCCTCTTCTGATTGGGAAGACTATTCATTGGGGAAGGAAGGTGCAGAAAGGTACAGAATTCACAATCTTCCTAAAGTTTCAGGTCCTGGATTGTATGAGCTTGGCATTACTGTATCTAGCTCCGGTTTAGGTCGTGAGATAGCTAAACTAGACGCAGACTGGATAGTTGTTGTCTACCTTGGCGAAGCTGACAACGTGAGAACACGATTACAGCACTATGGTCGCTCGGGTTCTCATTTGGGCAATGCCTATTTCTGTGTTGTTGACTGTAAGGTTTTTCCTCTTGAAAAGGGACCCAGTTTATTTCAAGAAATGTTCTCAAGAGGGTACTCCATTGTGTATAGATGGGCTCCTATGAAAAACAAGAAGAATGCTCAGATGACTGAAACTCAACTGCTTAAAACATTTGACTATGCATGGAATACAAGTGGTAATGGTGCACGGCGCCATGACGATGTACTAAAAAAACTTGAGAACATCGCTTCACAAACTACAAAATCTACCTTTATTTCCAGAAAGCTTCTGCCTTTTACACAGAAGAAAATGGGCATCAAAATTAAAACAAGCAAGTCAATTCCAATGGTGAACAAACCAGCTGAAGATGCTGAGGAGAGGAACAATTTCTTTTCTCGCATTCTCAGTTTCAGCAGATCTCGGCCTAGACTTGTTGACAACACTAATGATGTTAATTGGGCAGATAGCAATAGCTGCGGAGTGGTCATAGGTCATAACGGTGAGGTTTGTAGGAAGCCCCCAGTTGAAGGAAGAAAAAGGTGTGCTGAACATAAAGGGATGAAGATTAATGGATTACTAAAGAACTCATCTAGCAGATTTATTTTACAAAAGCCCGTGAATGTAGGAACAACCATATATGGTGAAAAAGACTTCTCTTGTAGTAAATCTGAGATTCCCAATAGCACAGAAGAATGCTCAGTTTCGAATTCATTTCCCAATAAGGGGAGTTCTCTTCCAATTTGTGGAGTTGTTCTGTATGATGGCTCTCCATGTAGAAGACCCCCAGTTCAAGGAAGGAAACGGTGTGAAGAACACAAAGGGCAACGAATTTGTAGATCGACTTTAGTCACTTCCAAGTACCAGCAAACTTCACCCATATCAAAACCTGAGTCAACTGTGATAGCACAAGGCACTTCTTCAGAACTTTCTAGCTCAGGTCTCGAAAGAATGTGTGGTGTTGATTTAGGCAATGGACTCAACTGCACTAGGCAGCCAGTGAAAGGACGAGTTAGGTGTGGGGAACATAAAGGAATGAGAACTAATAAGTTGATTTCAACGTTAGCCACAAGCAACAAACCGGATGTGTCCGATACCGGTTCCGTCAGCTAA

Protein sequence

MVAELSPIRLKREDCKRTKHDSDFSKWEILVASSDWEDYSLGKEGAERYRIHNLPKVSGPGLYELGITVSSSGLGREIAKLDADWIVVVYLGEADNVRTRLQHYGRSGSHLGNAYFCVVDCKVFPLEKGPSLFQEMFSRGYSIVYRWAPMKNKKNAQMTETQLLKTFDYAWNTSGNGARRHDDVLKKLENIASQTTKSTFISRKLLPFTQKKMGIKIKTSKSIPMVNKPAEDAEERNNFFSRILSFSRSRPRLVDNTNDVNWADSNSCGVVIGHNGEVCRKPPVEGRKRCAEHKGMKINGLLKNSSSRFILQKPVNVGTTIYGEKDFSCSKSEIPNSTEECSVSNSFPNKGSSLPICGVVLYDGSPCRRPPVQGRKRCEEHKGQRICRSTLVTSKYQQTSPISKPESTVIAQGTSSELSSSGLERMCGVDLGNGLNCTRQPVKGRVRCGEHKGMRTNKLISTLATSNKPDVSDTGSVS*
BLAST of Cucsa.397230 vs. Swiss-Prot
Match: ET2_ARATH (Protein EFFECTOR OF TRANSCRIPTION 2 OS=Arabidopsis thaliana GN=ET2 PE=1 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 4.3e-83
Identity = 186/457 (40.70%), Postives = 260/457 (56.89%), Query Frame = 1

Query: 7   PIRLKREDCKRTKHDSDFSKWEILVASSDWEDYSLGKEGAERYRIHNLPKVSGPGLYELG 66
           P   KRED KRTKHD+ FSKW++L+ S+DWED+  GK+G  RYR+ NLP+ S PGLYELG
Sbjct: 13  PTVFKREDYKRTKHDTVFSKWQVLIGSNDWEDFKNGKDGVGRYRVQNLPRKSCPGLYELG 72

Query: 67  ITVSSSGLGRE-IAKLDADWIVVVYLGEADNVRTRLQHYGRSGSHLGNAYFCVVDCKVF- 126
           + V    +G+E   KL+ D ++  YLG+A++VR+RLQ YGRSG+HL N    + DC+   
Sbjct: 73  VAV----IGQEQCRKLEPDIVLASYLGQAESVRSRLQRYGRSGAHLRNVNN-LNDCETIE 132

Query: 127 -PLEKGPS-LFQEMFSRGYSIVYRWAPMKNKKNAQMTETQLLKTFDYAWNTSGNGARRHD 186
            P++     LF+++FS+G SI+YRWAPM +K+ A+ TE  LL TFDYAWN   NG RR  
Sbjct: 133 SPVKAVTGGLFEDIFSKGGSILYRWAPMGSKREAEATEGMLLSTFDYAWNKGSNGERRQL 192

Query: 187 DVLKKLENIASQTTKSTFISRKLLPFTQKKMGIKIKTSKSIPMVNKPAE---DAEERNNF 246
           D+LKKL +    + + + ISR L PF + ++GI+IK  K +    +      D E+ NNF
Sbjct: 193 DLLKKLGDREFMSKRKSGISRMLFPFLRNQVGIRIKGEKHVLKEERKLTCDVDEEKSNNF 252

Query: 247 FSRILSFSRSRPRLV-DNTNDVNWADSN-SCGVVIGHNGEVCRKPPVEGRKRCAEHKGMK 306
            + IL  +RSRP+ V D  ++V+ + S+  CGV++  +G  C + PV+GRKRC EHKG +
Sbjct: 253 LTSILKLTRSRPQPVSDRFDEVDGSCSDIVCGVLL-EDGGCCIRSPVKGRKRCIEHKGQR 312

Query: 307 INGLLKNSSSRFILQKPVNVGTTIYGEKDFSCSKSEIPNSTEECSVSNSFPNKGSSLPIC 366
           +  +                          S  K   P S  E        N   S  +C
Sbjct: 313 VCRV--------------------------SPEKQTPPKS--EIFTGQDHHNHKDSDVVC 372

Query: 367 GVVLYDGSPCRRPPVQGRKRCEEHKGQRICRSTLVTSK--YQQTSPISKPESTVIAQGTS 426
           GV+L D  PC + PV GRKRCE+HKG RI     + ++   ++T    KP+     +  +
Sbjct: 373 GVILPDMEPCNKRPVPGRKRCEDHKGMRINAFLFLLNQTDREKTVKDEKPD----PESHT 431

Query: 427 SELSSSGLERMCGVDLGNGLNCTRQPVKGRVRCGEHK 453
             +    L R C     NGL CTR   KG  RC +HK
Sbjct: 433 ESIEEEALTRFCEATTKNGLPCTRSSPKGSKRCWQHK 431


HSP 2 Score: 92.0 bits (227), Expect = 1.8e-17
Identity = 72/189 (38.10%), Postives = 94/189 (49.74%), Query Frame = 1

Query: 268 CGVVIGHNGEVCRKPPVEGRKRCAEHKGMKINGLLKNSSSRFILQKPVNVGTTIYGEKDF 327
           CGV++  + E C K PV GRKRC +HKGM+IN  L      F+L +  +   T+  EK  
Sbjct: 338 CGVILP-DMEPCNKRPVPGRKRCEDHKGMRINAFL------FLLNQ-TDREKTVKDEKPD 397

Query: 328 SCSKSEIPNSTEECSVSNSFPNKGSSLPICGVVLYDGSPCRRPPVQGRKRCEEHKGQRIC 387
             S +E   S EE +++            C     +G PC R   +G KRC +HK +   
Sbjct: 398 PESHTE---SIEEEALTR----------FCEATTKNGLPCTRSSPKGSKRCWQHKEK--- 457

Query: 388 RSTLVTSKYQQTSPIS-KPESTVIAQGTSSELSSSGLERMCGVDLGNGLNCTRQPVKGRV 447
                TS    TSP+  +PE+   A+  +           CGV LGNGL C R PVKGR 
Sbjct: 458 -----TSS--DTSPVYFQPEA---AKNVA-----------CGVKLGNGLICERSPVKGRK 481

Query: 448 RCGEHKGMR 456
           RC EHKGMR
Sbjct: 518 RCEEHKGMR 481


HSP 3 Score: 84.0 bits (206), Expect = 5.0e-15
Identity = 48/113 (42.48%), Postives = 65/113 (57.52%), Query Frame = 1

Query: 356 ICGVVLYDGSPCRRPPVQGRKRCEEHKGQRICRSTLVTSKYQQTSPISKPESTVIAQGTS 415
           +CGV+L DG  C R PV+GRKRC EHKGQR+CR     S  +QT P S+     I  G  
Sbjct: 277 VCGVLLEDGGCCIRSPVKGRKRCIEHKGQRVCR----VSPEKQTPPKSE-----IFTGQD 336

Query: 416 SELSSSGLERMCGVDLGNGLNCTRQPVKGRVRCGEHKGMRTNKLISTLATSNK 469
              +    + +CGV L +   C ++PV GR RC +HKGMR N  +  L  +++
Sbjct: 337 HH-NHKDSDVVCGVILPDMEPCNKRPVPGRKRCEDHKGMRINAFLFLLNQTDR 379


HSP 4 Score: 48.1 bits (113), Expect = 3.1e-04
Identity = 21/36 (58.33%), Postives = 28/36 (77.78%), Query Frame = 1

Query: 263 ADSNSCGVVIGHNGEVCRKPPVEGRKRCAEHKGMKI 299
           A + +CGV +G NG +C + PV+GRKRC EHKGM+I
Sbjct: 448 AKNVACGVKLG-NGLICERSPVKGRKRCEEHKGMRI 482


HSP 5 Score: 44.3 bits (103), Expect = 4.4e-03
Identity = 23/55 (41.82%), Postives = 31/55 (56.36%), Query Frame = 1

Query: 417 ELSSSGLERMCGVDLGNGLNCTRQPVKGRVRCGEHKGMRTNKLISTLATSNKPDV 472
           E+  S  + +CGV L +G  C R PVKGR RC EHKG R  ++     T  K ++
Sbjct: 268 EVDGSCSDIVCGVLLEDGGCCIRSPVKGRKRCIEHKGQRVCRVSPEKQTPPKSEI 322

BLAST of Cucsa.397230 vs. Swiss-Prot
Match: ET3_ARATH (Protein EFFECTOR OF TRANSCRIPTION 3 OS=Arabidopsis thaliana GN=ET3 PE=4 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 5.0e-31
Identity = 86/217 (39.63%), Postives = 115/217 (53.00%), Query Frame = 1

Query: 7   PIRLKREDCKRTKHDSDFSKWEILVASSDWEDYSLGKEGAERYRIHNLPKVSGPGLYELG 66
           P   KRED K T HD  FSKW  L+  +DW+D++  KE   RYR  +LP     GLYELG
Sbjct: 52  PTMFKREDYKLTIHDIAFSKWRNLIRHNDWKDFNNRKERVRRYRHEDLPPQRCTGLYELG 111

Query: 67  ITVSSSGLGREIAKLDADWIVV-VYLGEADNVRTRLQHYGRSGSHLGNAYFCVVDCKVFP 126
           + V     G+     D D  V+ VY+G+  +V++RLQ YGR G HL +            
Sbjct: 112 VGVIGQDQGQ---NFDPDNNVLGVYVGQCVDVKSRLQDYGRRGGHLPS------------ 171

Query: 127 LEKGPSLFQEMFSRGYSIVYRWAPMKNKKNAQMTETQLLKTFDYAWNTSGNGARRHDDVL 186
                 L++++FS GYS+ YRWAP      A  TE  LL TFDYAWNT  NG RRH    
Sbjct: 172 -----GLYEDIFSEGYSVFYRWAP-----EAAATEGMLLSTFDYAWNTCSNGERRH---- 231

Query: 187 KKLENIASQTTKSTFISRKLLPFTQKKMGIKIKTSKS 223
            +L+ +      S   S+ L+P  + ++ + IK  KS
Sbjct: 232 LELQKLGDPEFMSKRKSQVLVPSIRDQV-VTIKVEKS 238

BLAST of Cucsa.397230 vs. Swiss-Prot
Match: ET1_ARATH (Protein EFFECTOR OF TRANSCRIPTION 1 OS=Arabidopsis thaliana GN=ET1 PE=2 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 2.9e-23
Identity = 83/262 (31.68%), Postives = 126/262 (48.09%), Query Frame = 1

Query: 201 ISRKLLPFTQKKMGIKIKTSKSIPMVNKP--AEDAEERNNFFSRILSFSRSRPRLVDNTN 260
           +S+ +LP TQ K   + +  KS     +   ++ AE+ +N    IL  SRSRP+ V   +
Sbjct: 234 LSQTILPTTQNKSDNQTEEKKSDSEEEREVSSDAAEKESNSLPSILRLSRSRPQPVSEKH 293

Query: 261 D--VNWADSNS-CGVVIGHNGEVCRKPPVEGRKRCAEHKGMKINGLLKNSSSRFILQKPV 320
           D  V+ +DS S CGV++  +G  C   PV+GRKRC EHKG +++               V
Sbjct: 294 DDIVDESDSASACGVLL-EDGTTCTTTPVKGRKRCTEHKGKRLSR--------------V 353

Query: 321 NVGTTIYGEKDFSCSKSEIPNSTEECSVSNSFPNKGSSLPICGVVLYDGSPCRRPPVQGR 380
           + G  I           E+P +  EC  + +         ICGV+L D   CR  PV  R
Sbjct: 354 SPGIHI---------PCEVP-TVRECEETEN---------ICGVILPDMIRCRSKPVSRR 413

Query: 381 KRCEEHKGQRICRSTLVTSKYQQTSPISKPESTVIAQGTSSELSSSGLERMCGVDLGNGL 440
           KRCE+HKG R+     + +  ++   +++ +S      TS+ ++  G   +C     NGL
Sbjct: 414 KRCEDHKGMRVNAFFFLLNPTERDKAVNEDKS---KPETSTGMNQEGSGLLCEATTKNGL 458

Query: 441 NCTRQPVKGRVRCGEHKGMRTN 458
            CTR   +G  RC +HK    N
Sbjct: 474 PCTRSAPEGSKRCWQHKDKTLN 458


HSP 2 Score: 84.3 bits (207), Expect = 3.8e-15
Identity = 61/190 (32.11%), Postives = 82/190 (43.16%), Query Frame = 1

Query: 266 NSCGVVIGHNGEVCRKPPVEGRKRCAEHKGMKINGLLKNSSSRFILQKPVNVGTTIYGEK 325
           N CGV++      CR  PV  RKRC +HKGM++N         F L  P      +  +K
Sbjct: 359 NICGVILPDMIR-CRSKPVSRRKRCEDHKGMRVNAF-------FFLLNPTERDKAVNEDK 418

Query: 326 DFSCSKSEIPNSTEECSVSNSFPNKGSSLPICGVVLYDGSPCRRPPVQGRKRCEEHKGQR 385
               SK E          S     +GS L +C     +G PC R   +G KRC +HK + 
Sbjct: 419 ----SKPE---------TSTGMNQEGSGL-LCEATTKNGLPCTRSAPEGSKRCWQHKDKT 478

Query: 386 ICRSTLVTSKYQQTSPISKPESTVIAQGTSSELSSSGLERMCGVDLGNGLNCTRQPVKGR 445
           +   +                S  +   T+S++       +CG  L NG  C + PVKGR
Sbjct: 479 LNHGS----------------SENVQSATASQV-------ICGFKLYNGSVCEKSPVKGR 503

Query: 446 VRCGEHKGMR 456
            RC EHKGMR
Sbjct: 539 KRCEEHKGMR 503

BLAST of Cucsa.397230 vs. Swiss-Prot
Match: ET_BRANA (Protein EFFECTOR OF TRANSCRIPTION OS=Brassica napus GN=ET PE=2 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 2.0e-16
Identity = 64/188 (34.04%), Postives = 82/188 (43.62%), Query Frame = 1

Query: 268 CGVVIGHNGEVCRKPPVEGRKRCAEHKGMKINGLLKNSSSRFILQKPVNVGTTIYGEKDF 327
           CGV++      CR  PV GRKRC +HKGM++N         F L  P         E+D 
Sbjct: 295 CGVILPEMVR-CRSKPVSGRKRCEDHKGMRVNAF-------FFLLNPT--------ERDK 354

Query: 328 SCSKSEIPNSTEECSVSNSFPNKGSSLPICGVVLYDGSPCRRPPVQGRKRCEEHKGQRIC 387
              + +    T   S +   P  G SL IC     +G PC R    G KRC +HK + + 
Sbjct: 355 ILKEDKSKPKTRTSSTNQEEP--GESL-ICEATTKNGLPCTRSAPNGSKRCWQHKDETVD 414

Query: 388 RSTLVTSKYQQTSPISKPESTVIAQGTSSELSSSGLERMCGVDLGNGLNCTRQPVKGRVR 447
           + +   S+  QTS                         +CGV L NG  C + PVKGR R
Sbjct: 415 QKS---SENVQTSTT-----------------------VCGVKLHNGSVCEKTPVKGRKR 437

Query: 448 CGEHKGMR 456
           C EHKGMR
Sbjct: 475 CQEHKGMR 437


HSP 2 Score: 75.9 bits (185), Expect = 1.4e-12
Identity = 75/275 (27.27%), Postives = 108/275 (39.27%), Query Frame = 1

Query: 184 VLKKLENIASQTTKSTFISRKLLPFTQKKMGIKIKTSKSIPMVNKPAEDAEERNNFFSRI 243
           +LKKL + ASQT             TQKK G + K  K      +     E   N  + +
Sbjct: 168 LLKKLGDNASQT------------ITQKKTGGRSKDKKHGFEEERDVSRVEAEENNTNSV 227

Query: 244 ------LSFSRSRPRLVDNTNDVNWADSNSCGVVIGHNGEVCRKPPVEGRKRCAEHKGMK 303
                 LS SR +P L  + + V+ +DS S   V+  +G  C   PV GRKRC EHKG +
Sbjct: 228 HASVLRLSRSRPQPVLERHDDIVDGSDSASVCGVLQEDGTTCLTAPVTGRKRCTEHKGQR 287

Query: 304 INGLLKNSSSRFILQKPVNVGTTIYGEKDFSCSKSEIPNSTEECSVSNSFPNKGSSLPIC 363
           I               PV         K+  C +     + E C V      +  S P+ 
Sbjct: 288 IT-----------CAPPV---------KNPPCEE----ETEEICGVILPEMVRCRSKPVS 347

Query: 364 GVVLYDGSPCRRPPVQGRKRCEEHKGQRICRSTLVTSKYQQTSPISKPESTVIAQGTSSE 423
           G                 KRCE+HKG R+     + +  ++   + + +S    + +S+ 
Sbjct: 348 GR----------------KRCEDHKGMRVNAFFFLLNPTERDKILKEDKSKPKTRTSSTN 390

Query: 424 LSSSGLERMCGVDLGNGLNCTRQPVKGRVRCGEHK 453
               G   +C     NGL CTR    G  RC +HK
Sbjct: 408 QEEPGESLICEATTKNGLPCTRSAPNGSKRCWQHK 390


HSP 3 Score: 73.6 bits (179), Expect = 6.8e-12
Identity = 42/119 (35.29%), Postives = 54/119 (45.38%), Query Frame = 1

Query: 353 SLPICGVVLYDGSPCRRPPVQGRKRCEEHKGQRICRSTLVTSKYQQTSPISKPESTVIAQ 412
           S  +CGV+  DG+ C   PV GRKRC EHKGQRI  +           P+  P       
Sbjct: 243 SASVCGVLQEDGTTCLTAPVTGRKRCTEHKGQRITCA----------PPVKNPP------ 302

Query: 413 GTSSELSSSGLERMCGVDLGNGLNCTRQPVKGRVRCGEHKGMRTNKLISTLATSNKPDV 472
                      E +CGV L   + C  +PV GR RC +HKGMR N     L  + +  +
Sbjct: 303 ------CEEETEEICGVILPEMVRCRSKPVSGRKRCEDHKGMRVNAFFFLLNPTERDKI 339


HSP 4 Score: 51.6 bits (122), Expect = 2.8e-05
Identity = 26/46 (56.52%), Postives = 31/46 (67.39%), Query Frame = 1

Query: 254 VDNTNDVNWADSNS-CGVVIGHNGEVCRKPPVEGRKRCAEHKGMKI 299
           VD  +  N   S + CGV + HNG VC K PV+GRKRC EHKGM+I
Sbjct: 394 VDQKSSENVQTSTTVCGVKL-HNGSVCEKTPVKGRKRCQEHKGMRI 438

BLAST of Cucsa.397230 vs. TrEMBL
Match: A0A0A0LWE7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G586820 PE=4 SV=1)

HSP 1 Score: 974.9 bits (2519), Expect = 3.5e-281
Identity = 478/478 (100.00%), Postives = 478/478 (100.00%), Query Frame = 1

Query: 1   MVAELSPIRLKREDCKRTKHDSDFSKWEILVASSDWEDYSLGKEGAERYRIHNLPKVSGP 60
           MVAELSPIRLKREDCKRTKHDSDFSKWEILVASSDWEDYSLGKEGAERYRIHNLPKVSGP
Sbjct: 1   MVAELSPIRLKREDCKRTKHDSDFSKWEILVASSDWEDYSLGKEGAERYRIHNLPKVSGP 60

Query: 61  GLYELGITVSSSGLGREIAKLDADWIVVVYLGEADNVRTRLQHYGRSGSHLGNAYFCVVD 120
           GLYELGITVSSSGLGREIAKLDADWIVVVYLGEADNVRTRLQHYGRSGSHLGNAYFCVVD
Sbjct: 61  GLYELGITVSSSGLGREIAKLDADWIVVVYLGEADNVRTRLQHYGRSGSHLGNAYFCVVD 120

Query: 121 CKVFPLEKGPSLFQEMFSRGYSIVYRWAPMKNKKNAQMTETQLLKTFDYAWNTSGNGARR 180
           CKVFPLEKGPSLFQEMFSRGYSIVYRWAPMKNKKNAQMTETQLLKTFDYAWNTSGNGARR
Sbjct: 121 CKVFPLEKGPSLFQEMFSRGYSIVYRWAPMKNKKNAQMTETQLLKTFDYAWNTSGNGARR 180

Query: 181 HDDVLKKLENIASQTTKSTFISRKLLPFTQKKMGIKIKTSKSIPMVNKPAEDAEERNNFF 240
           HDDVLKKLENIASQTTKSTFISRKLLPFTQKKMGIKIKTSKSIPMVNKPAEDAEERNNFF
Sbjct: 181 HDDVLKKLENIASQTTKSTFISRKLLPFTQKKMGIKIKTSKSIPMVNKPAEDAEERNNFF 240

Query: 241 SRILSFSRSRPRLVDNTNDVNWADSNSCGVVIGHNGEVCRKPPVEGRKRCAEHKGMKING 300
           SRILSFSRSRPRLVDNTNDVNWADSNSCGVVIGHNGEVCRKPPVEGRKRCAEHKGMKING
Sbjct: 241 SRILSFSRSRPRLVDNTNDVNWADSNSCGVVIGHNGEVCRKPPVEGRKRCAEHKGMKING 300

Query: 301 LLKNSSSRFILQKPVNVGTTIYGEKDFSCSKSEIPNSTEECSVSNSFPNKGSSLPICGVV 360
           LLKNSSSRFILQKPVNVGTTIYGEKDFSCSKSEIPNSTEECSVSNSFPNKGSSLPICGVV
Sbjct: 301 LLKNSSSRFILQKPVNVGTTIYGEKDFSCSKSEIPNSTEECSVSNSFPNKGSSLPICGVV 360

Query: 361 LYDGSPCRRPPVQGRKRCEEHKGQRICRSTLVTSKYQQTSPISKPESTVIAQGTSSELSS 420
           LYDGSPCRRPPVQGRKRCEEHKGQRICRSTLVTSKYQQTSPISKPESTVIAQGTSSELSS
Sbjct: 361 LYDGSPCRRPPVQGRKRCEEHKGQRICRSTLVTSKYQQTSPISKPESTVIAQGTSSELSS 420

Query: 421 SGLERMCGVDLGNGLNCTRQPVKGRVRCGEHKGMRTNKLISTLATSNKPDVSDTGSVS 479
           SGLERMCGVDLGNGLNCTRQPVKGRVRCGEHKGMRTNKLISTLATSNKPDVSDTGSVS
Sbjct: 421 SGLERMCGVDLGNGLNCTRQPVKGRVRCGEHKGMRTNKLISTLATSNKPDVSDTGSVS 478

BLAST of Cucsa.397230 vs. TrEMBL
Match: W9R5X1_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_027255 PE=4 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 2.9e-126
Identity = 246/476 (51.68%), Postives = 321/476 (67.44%), Query Frame = 1

Query: 9   RLKREDCKRTKHDSDFSKWEILVASSDWEDYSLGKEGAERYRIHNLPKVSGPGLYELGIT 68
           RLKREDCKRTKHD +FSKWEIL+  SDWE+Y LGKEGAERYR+HNLPK SGPG+YELGI 
Sbjct: 63  RLKREDCKRTKHDREFSKWEILIGPSDWEEYLLGKEGAERYRVHNLPKNSGPGVYELGIA 122

Query: 69  VSSSGLGREIAKLDADWIVVVYLGEADNVRTRLQHYGRSGSHLGNAYFCVVDCKVFPLEK 128
           VS +GLGREI KLD + IVVVYLG+ADNVRTRLQHYGR+G+HLGN+  C   CK    +K
Sbjct: 123 VSRTGLGREIGKLDPERIVVVYLGQADNVRTRLQHYGRTGAHLGNS--CPPQCKTVIPQK 182

Query: 129 GPSLFQEMFSRGYSIVYRWAPMKNKKNAQMTETQLLKTFDYAWNTSGNGARRHDDVLKKL 188
           GP LF+E+ SRGY I++RWAPM++K +A  TET+LL TFDYAWNTS NG RR DD+L KL
Sbjct: 183 GPGLFEEILSRGYPIIFRWAPMQSKSDALKTETELLNTFDYAWNTSINGTRRPDDILNKL 242

Query: 189 ENIASQTTKSTFISRKLLPFTQKKMGIKIKTSKSIPMVNKPAEDA-EERNNFFSRILSFS 248
           + I+S     + I+RKL+PF+QK++GI+IK  K      K    A EE  N FSR+  F 
Sbjct: 243 KKISSSNIHFSDIARKLVPFSQKQVGIRIKARKFPSAEEKIDTSADEESQNIFSRVFKFG 302

Query: 249 RSRPRLVDNTNDVNWADSNSCGVVIGHNGEVCRKPPVEGRKRCAEHKGMKINGLLKNSSS 308
           RS+P+LV + + +   D+  CG+V+G +  +CR+PPVEGRKRCAEHKGM+I G       
Sbjct: 303 RSQPKLVLDRSVIVLEDTTICGMVLGLD-SICRRPPVEGRKRCAEHKGMRIKG------- 362

Query: 309 RFILQKPVNV---GTTIYGEKDFSCSKSEIPNSTEECSVSNSFPNKGSSLPICGVVLYDG 368
                 PV+V    + +  +  FS   S + +S E   V+ +  ++ S  PICG +L DG
Sbjct: 363 ------PVSVALSNSNVNSQSTFSAPSSSL-HSEELQPVTFNNASRESFTPICGFILADG 422

Query: 369 SPCRRPPVQGRKRCEEHKGQRICRSTLVTS-----KYQQTSPISKPESTVI-------AQ 428
           SPC   P++G KRC EHKG++I + T   S      Y    P+S+ +S+ I       AQ
Sbjct: 423 SPCISQPLKGNKRCLEHKGRKILQQTNSASVTKEKLYYLPDPVSESKSSEICFSGEVQAQ 482

Query: 429 GTSSELSSSGLERMCGVDLGNGLNCTRQPVKGRVRCGEHKGMRTNKLISTLATSNK 469
             +  L+++  + +CGV LG+GL CTR+PV GRVRC EHKG+R  +  +T   + K
Sbjct: 483 AFAKRLNAN-CDTICGVVLGDGLVCTRKPVVGRVRCEEHKGIRVTRFATTKFAAEK 520

BLAST of Cucsa.397230 vs. TrEMBL
Match: W9R5X1_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_027255 PE=4 SV=1)

HSP 1 Score: 43.5 bits (101), Expect = 8.4e-01
Identity = 21/51 (41.18%), Postives = 29/51 (56.86%), Query Frame = 1

Query: 426 MCGVDLGNGLNCTRQPVKGRVRCGEHKGMRTNKLISTLATSNKPDVSDTGS 477
           +CG+ LG    C R PV+GR RC EHKGMR    +S   +++  +   T S
Sbjct: 320 ICGMVLGLDSICRRPPVEGRKRCAEHKGMRIKGPVSVALSNSNVNSQSTFS 370


HSP 2 Score: 422.9 bits (1086), Expect = 5.1e-115
Identity = 248/493 (50.30%), Postives = 314/493 (63.69%), Query Frame = 1

Query: 1   MVAELSPI--RLKREDCKRTKHDSDFSKWEILVASSDWEDYSLGKEGAERYRIHNLPKVS 60
           MV    PI  RLKREDCKRTKHDS+F+KW+ILV  SDWED+S  KEGAERYR+HNLP   
Sbjct: 1   MVTTEPPIVSRLKREDCKRTKHDSEFTKWKILVGPSDWEDHSSRKEGAERYRVHNLPLCC 60

Query: 61  G-PGLYELGITVSSSGLGREIAKLDADWIVVVYLGEADNVRTRLQHYGRSGSHL------ 120
             PG+YELGI+VS +GLGRE+ KLD   IVVVYLG+A+++R RLQHYGRSG+HL      
Sbjct: 61  HCPGVYELGISVSPTGLGREVGKLDPAHIVVVYLGQAEDIRARLQHYGRSGAHLAKVDST 120

Query: 121 GNAYFCVVDCKVFPLEKGPSLFQEMFSRGYSIVYRWAPMKNKKNAQMTETQLLKTFDYAW 180
           GN      +CK  PL++G  LF+ +FSRGYSIV+RWAP+++K++A+ TE++LLKTFDYAW
Sbjct: 121 GNPN----NCKNMPLQRGSGLFEGIFSRGYSIVFRWAPIESKRDAERTESRLLKTFDYAW 180

Query: 181 NTSGNGARRHDDVLKKLENIASQTTKSTFISRKLLPFTQKKMGIKIKTSKSIPMVNKPAE 240
           N   NGARR  DVL+KLE  +S T     I+RK     QK++GI IK SK     N  + 
Sbjct: 181 NKGSNGARRPSDVLRKLEKTSSSTPPFPSITRKFQMLGQKQVGIIIKASKPFLQENGLST 240

Query: 241 DAEERNNFFSRILSFSRSRPRLVDNTNDVNWADSNSCGVVIGHNGEVCRKPPVEGRKRCA 300
           D ++ N+  +R+  F RS+PRLV +   V    +++CGV +G +G +C +PPVEGRKRCA
Sbjct: 241 D-QDINSLLARVFKFGRSQPRLVSDRVGVTGNYNSTCGVALG-DGTICERPPVEGRKRCA 300

Query: 301 EHKGMKINGLLKNSSSRFILQ-KPVNVGTTIYGEKDFSCSKSEIPNST---EECSVSNSF 360
           EHKGMK     K+S S+ I++ KP  V      E   + S     N      +C V+  F
Sbjct: 301 EHKGMK----TKSSFSKPIVERKPQMVDLNRESEGHSAQSYGYTVNFQLVGGKCPVNKDF 360

Query: 361 PNKGSSLPICGVVLYDGSPCRRPPVQGRKRCEEHKGQR----ICRSTLVTSKYQQTSPIS 420
                   ICGV+L DG PC+R PV GRKRCEEHKG R    I  ST+  S +      S
Sbjct: 361 ------TIICGVILDDGFPCKRQPVLGRKRCEEHKGMRTNVSISNSTVGKSHHTYE---S 420

Query: 421 KPESTVIAQGTSSELSSSGLERMCGVDLGNGLNCTRQPVKGRVRCGEHKGMRTNKLISTL 477
           K +  V+    S   +  G+   CGVDLG+G+ C R  V GR RC EHKGMR N LISTL
Sbjct: 421 KLQPQVLPDCRSFSNTDYGI--TCGVDLGHGIFCKRLAVAGRKRCEEHKGMRINSLISTL 472

BLAST of Cucsa.397230 vs. TrEMBL
Match: D7TB99_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_11s0016g02440 PE=4 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 2.1e-20
Identity = 85/246 (34.55%), Postives = 114/246 (46.34%), Query Frame = 1

Query: 214 GIKIKTSKSIPMVNKPAE--DAEERNNFFSRILSFSRSRPRLVDNTNDVNWADSNSCGVV 273
           G+K K+S S P+V +  +  D    +   S          +LV     VN   +  CGV+
Sbjct: 298 GMKTKSSFSKPIVERKPQMVDLNRESEGHSAQSYGYTVNFQLVGGKCPVNKDFTIICGVI 357

Query: 274 IGHNGEVCRKPPVEGRKRCAEHKGMKINGLLKNSSSRFILQKPVNVGTTIYGEKDFSCSK 333
           +  +G  C++ PV GRKRC EHKGM+ N  + NS+                G+   +   
Sbjct: 358 LD-DGFPCKRQPVLGRKRCEEHKGMRTNVSISNST---------------VGKSHHTYES 417

Query: 334 SEIPNSTEECSVSNSFPNKGSSLPICGVVLYDGSPCRRPPVQGRKRCEEHKGQRICRSTL 393
              P    +C    SF N    +  CGV L  G  C+R  V GRKRCEEHKG RI  S +
Sbjct: 418 KLQPQVLPDC---RSFSNTDYGIT-CGVDLGHGIFCKRLAVAGRKRCEEHKGMRI-NSLI 477

Query: 394 VTSKYQQTSPISKPESTVIAQGTSSELSSSGLERMCGVDLGNGLNCTRQPVKGRVRCGEH 453
            T   +  S      S  I    S+   +S     CG+ LG+G  C RQPV+G  RC +H
Sbjct: 478 STLAGEDKSHALDMGSGFITYNNSNYNHTS----TCGMTLGDGSICKRQPVEGNKRCWQH 518

Query: 454 KGMRTN 458
           KGMR N
Sbjct: 538 KGMRVN 518


HSP 2 Score: 99.0 bits (245), Expect = 1.7e-17
Identity = 72/211 (34.12%), Postives = 102/211 (48.34%), Query Frame = 1

Query: 247 SRSRPRLVDNTNDVNWADSN-SCGVVIGHNGEVCRKPPVEGRKRCAEHKGMKINGLLKNS 306
           S+ +P+++ +    +  D   +CGV +GH G  C++  V GRKRC EHKGM+IN L+   
Sbjct: 401 SKLQPQVLPDCRSFSNTDYGITCGVDLGH-GIFCKRLAVAGRKRCEEHKGMRINSLI--- 460

Query: 307 SSRFILQKPVNVGTTIYGEKDFSCSKSEIPNSTEECSVSNSFPNKGSSLPICGVVLYDGS 366
                        +T+ GE         +   +   + +NS  N  S+   CG+ L DGS
Sbjct: 461 -------------STLAGED----KSHALDMGSGFITYNNSNYNHTST---CGMTLGDGS 520

Query: 367 PCRRPPVQGRKRCEEHKGQRI-CRSTLVTSKYQQTSPISKPESTVIAQGTSSELSSSGLE 426
            C+R PV+G KRC +HKG R+ C S+   S           E+T                
Sbjct: 521 ICKRQPVEGNKRCWQHKGMRVNCSSSWFGS-----------ETT---------------- 559

Query: 427 RMCGVDLGNGLNCTRQPVKGRVRCGEHKGMR 456
            +CGV L NG  C+R P  GR RC +HKGMR
Sbjct: 581 -LCGVLLRNGSVCSRVPAHGRKRCEQHKGMR 559


HSP 3 Score: 413.3 bits (1061), Expect = 4.0e-112
Identity = 226/476 (47.48%), Postives = 294/476 (61.76%), Query Frame = 1

Query: 9   RLKREDCKRTKHDSDFSKWEILVASSDWEDYSLGKEGAERYRIHNLPKVSGPGLYELGIT 68
           RLKRE  KRTKHDS FSKW++L+   DW DYS+GKEG  RYR+ NLPK S  GLYEL I 
Sbjct: 14  RLKREGHKRTKHDSQFSKWKVLIGPDDWVDYSIGKEGVARYRVENLPKNSSSGLYELAIY 73

Query: 69  VSSSGLGREIAKLDADWIVVVYLGEADNVRTRLQHYGRSGSHLGNAYFCVVDCKVFPLEK 128
            S S       KLD   ++VVYLGEADNVRTRLQ YGR+G+HLG +            EK
Sbjct: 74  RSGSSSRENTGKLDRYKVLVVYLGEADNVRTRLQQYGRTGAHLGRS---------SSGEK 133

Query: 129 GPSLFQEMFSRGYSIVYRWAPMKNKKNAQMTETQLLKTFDYAWNTSGNGARRHDDVLKKL 188
           G   F+++FSRGYSIVYRWAPM+NK +AQ TE QLL TFDYAWN   NGARRHD++L KL
Sbjct: 134 GCGYFEDIFSRGYSIVYRWAPMENKADAQRTEAQLLNTFDYAWNKGSNGARRHDNILWKL 193

Query: 189 ENIASQTTKSTFISRKLLPFTQKKMGIKIKTSKSIPMVNKPAEDAE-ERNNFFSRILSFS 248
           +  AS  T     SRKLLPF QK++GIKIK SK +   ++ ++ ++ E  NF  ++  FS
Sbjct: 194 DKGASNRTPPAIFSRKLLPFHQKQVGIKIKASKLLSQDSEFSKYSDGEGYNFLWQVFKFS 253

Query: 249 RSRPRLVDNTNDVNWADSNSCGVVIGHNGEVCRKPPVEGRKRCAEHKGMKING--LLKNS 308
           RS+PRLV +    N  D+  CGVV+  +G +CR+PP+EGRKRCAEHKG K  G  +  ++
Sbjct: 254 RSQPRLVFDRGGSNENDTIICGVVL-VDGSICRRPPLEGRKRCAEHKGKKTRGSSVSLST 313

Query: 309 SSRFILQKPVNVGTTIYGEKDFSCSKSEIPN-STEECSVSNSFPNKGSSLPICGVVLYDG 368
           S +    K  +     +G  +++ + S+IP+  T    ++   P      PIC V + DG
Sbjct: 314 SEKSHTHKACS-DYVFFGNGEYNVNGSKIPSFGTVPSLIAGDCPAIERCSPICEVAMDDG 373

Query: 369 SPCRRPPVQGRKRCEEHKGQRICRSTLVTSKYQQTSPISKPESTVIAQGTSSELS----- 428
           S C R PV GRKRC+EHKG++IC S   T++YQ    ++       A G   + S     
Sbjct: 374 SLCTRQPVSGRKRCDEHKGRKICNSNSETTRYQTVPYVASDSYANDALGFDKKSSETFIR 433

Query: 429 --------------SSGLERMCGVDLGNGLNCTRQPVKGRVRCGEHKGMRTNKLIS 462
                         + G + +CGV+ GNG  CT+QPV+GRVRC EHKG+R   L+S
Sbjct: 434 GKVETGVAPHRPVFNDGCDTICGVEFGNGYFCTKQPVRGRVRCEEHKGLRVTSLLS 478

BLAST of Cucsa.397230 vs. TrEMBL
Match: A0A061GKU1_THECC (Effector of transcription2, putative OS=Theobroma cacao GN=TCM_037691 PE=4 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 5.8e-10
Identity = 64/199 (32.16%), Postives = 84/199 (42.21%), Query Frame = 1

Query: 275 NGEVCRKPPVEGRKRCAEHKGMKINGLLKNSSSRFILQKPVNVGTTIYGEKDFSCSKSEI 334
           +G +C + PV GRKRC EHKG KI     ++S     Q    V +  Y        K   
Sbjct: 361 DGSLCTRQPVSGRKRCDEHKGRKI---CNSNSETTRYQTVPYVASDSYANDALGFDKKSS 420

Query: 335 PNSTE---ECSVSNSFP--NKGSSLPICGVVLYDGSPCRRPPVQGRKRCEEHKGQRICRS 394
                   E  V+   P  N G    ICGV   +G  C + PV+GR RCEEHKG R+  S
Sbjct: 421 ETFIRGKVETGVAPHRPVFNDGCDT-ICGVEFGNGYFCTKQPVRGRVRCEEHKGLRV-TS 480

Query: 395 TLVTSKYQQTSPISKPESTVIAQGTSSELSSSGLERMCGVDLGNGLNCTRQPVKGRVRCG 454
            L     + TS     +    +       SSS    +CG    NG +C R  V G  RC 
Sbjct: 481 LLSGFDAKSTSHDFDMDPRFNSHNWKYGRSSSS-TTICGAPTRNGSSCQR-TVNGNGRCW 540

Query: 455 EHKGMRTNKLISTLATSNK 469
           +H     + + S+   S K
Sbjct: 541 QHSNYGCSSISSSSNFSTK 552


HSP 2 Score: 66.6 bits (161), Expect = 9.2e-08
Identity = 59/189 (31.22%), Postives = 72/189 (38.10%), Query Frame = 1

Query: 268 CGVVIGHNGEVCRKPPVEGRKRCAEHKGMKINGLL-----KNSSSRFILQKPVNVGTTIY 327
           CGV  G NG  C K PV GR RC EHKG+++  LL     K++S  F +    N     Y
Sbjct: 444 CGVEFG-NGYFCTKQPVRGRVRCEEHKGLRVTSLLSGFDAKSTSHDFDMDPRFNSHNWKY 503

Query: 328 GEKDFSCSKSEIPNSTEECSVSNSFPNKGSSLPICGVVLYDGSPCRRPPVQGRKRCEEHK 387
           G                            SS  ICG    +GS C+R  V G  RC +H 
Sbjct: 504 G-------------------------RSSSSTTICGAPTRNGSSCQR-TVNGNGRCWQH- 563

Query: 388 GQRICRSTLVTSKYQQTSPISKPESTVIAQGTSSELSSSGLERMCGVDLGNGLNCTRQPV 447
                 S    S    +S  S   S      TS          +CG    NG  C R+ V
Sbjct: 564 ------SNYGCSSISSSSNFSTKNSNYGDSSTS----------ICGAPTRNGSFCQRK-V 587

Query: 448 KGRVRCGEH 452
           KG  RC +H
Sbjct: 624 KGNGRCWQH 587


HSP 3 Score: 45.4 bits (106), Expect = 2.2e-01
Identity = 21/43 (48.84%), Postives = 28/43 (65.12%), Query Frame = 1

Query: 426 MCGVDLGNGLNCTRQPVKGRVRCGEHKGMRTNKLISTLATSNK 469
           +CGV L +G  C R P++GR RC EHKG +T     +L+TS K
Sbjct: 264 ICGVVLVDGSICRRPPLEGRKRCAEHKGKKTRGSSVSLSTSEK 306


HSP 4 Score: 408.3 bits (1048), Expect = 1.3e-110
Identity = 226/459 (49.24%), Postives = 294/459 (64.05%), Query Frame = 1

Query: 2   VAELSPIRLKREDCKRTKHDSDFSKWEILVASSDWEDYSLGKEGAERYRIHNLPKVSGPG 61
           +A ++  RLKRE C RTKHDS FS W+IL+  SDWED+S GKEG  RYRIHNLP+ S PG
Sbjct: 1   MAAVAVTRLKREQCNRTKHDSSFSHWKILIGPSDWEDHSRGKEGCARYRIHNLPQESNPG 60

Query: 62  LYELGITVSSSGLGREIAKL--DADWIVVVYLGEADNVRTRLQHYGRSGSHLGNAYFCVV 121
           +YELGI V+  GLGR+I+KL    D IVVVYLG+ADNVRTRLQ YGR+G+HL +A   + 
Sbjct: 61  VYELGIAVAGGGLGRQISKLAPHPDRIVVVYLGQADNVRTRLQCYGRTGAHLESA---IS 120

Query: 122 DCKVFPLEKGPSLFQEMFSRGYSIVYRWAPMKNKKNAQMTETQLLKTFDYAWNTSGNGAR 181
           D     L+KG  LFQ++FS+G+SIVYRWAP +NK++A  TE QLL+TFDYAWNTS NG R
Sbjct: 121 DDS--SLQKGLPLFQKVFSQGFSIVYRWAPTQNKEDALRTEDQLLRTFDYAWNTSNNGIR 180

Query: 182 RHDDVLKKLENIASQTTKSTFISRKLLPFTQKKMGIKIKTSKSIPMVNKPAEDAEERNNF 241
           R +++L+KL+ IAS T   + +++ LLPFTQK++GI+IK+ K       P +D     NF
Sbjct: 181 RPEEILQKLKKIASGTRTLSDVAKVLLPFTQKQVGIRIKSCK------LPQDDDNGSYNF 240

Query: 242 FSRILSFSRSRPRLVDNTNDVNWADSNS--CGVVIGHNGEVCRKPPVEGRKRCAEHKGMK 301
            +R+ SF+RSRPR+V +T+ V     N+  CGV +G +G +CR+PP E R RC+EHKGM+
Sbjct: 241 ITRVFSFNRSRPRIVQSTSGVVQEQENAKICGVSLG-DGLLCRRPPAEKRLRCSEHKGMR 300

Query: 302 INGLLKNSSSRFILQKPVNVGTTIYGEKDFSCSKSEIPNSTEECSVSNSFPNKGSSLPIC 361
            N               V++   I   K    S+S +P +     V  S  N      IC
Sbjct: 301 AN---------------VSIAKAISVAK----SESNVPQTVVGSPVDESMTNTN----IC 360

Query: 362 GVVLYDGSPCRRPPVQGRKRCEEHKGQRICRSTLVTSKYQQTSPISKPESTVIAQGTSSE 421
           G++L DGS CRR PV+GRKRC EHKG+RI  S        +   +S      +     S 
Sbjct: 361 GIILNDGSTCRRQPVEGRKRCHEHKGRRIGASVQKRGNGYRYQNVSHGVEDSLQTLVGSP 420

Query: 422 LSSSGLERMCGVDLGNGLNCTRQPVKGRVRCGEHKGMRT 457
           +  S    +CG+ L +G  CTRQPVKGR RC EHKG RT
Sbjct: 421 VDESISNTICGIILNDGSACTRQPVKGRKRCHEHKGRRT 424

BLAST of Cucsa.397230 vs. TAIR10
Match: AT5G56780.1 (AT5G56780.1 effector of transcription2)

HSP 1 Score: 310.1 bits (793), Expect = 2.4e-84
Identity = 186/457 (40.70%), Postives = 260/457 (56.89%), Query Frame = 1

Query: 7   PIRLKREDCKRTKHDSDFSKWEILVASSDWEDYSLGKEGAERYRIHNLPKVSGPGLYELG 66
           P   KRED KRTKHD+ FSKW++L+ S+DWED+  GK+G  RYR+ NLP+ S PGLYELG
Sbjct: 13  PTVFKREDYKRTKHDTVFSKWQVLIGSNDWEDFKNGKDGVGRYRVQNLPRKSCPGLYELG 72

Query: 67  ITVSSSGLGRE-IAKLDADWIVVVYLGEADNVRTRLQHYGRSGSHLGNAYFCVVDCKVF- 126
           + V    +G+E   KL+ D ++  YLG+A++VR+RLQ YGRSG+HL N    + DC+   
Sbjct: 73  VAV----IGQEQCRKLEPDIVLASYLGQAESVRSRLQRYGRSGAHLRNVNN-LNDCETIE 132

Query: 127 -PLEKGPS-LFQEMFSRGYSIVYRWAPMKNKKNAQMTETQLLKTFDYAWNTSGNGARRHD 186
            P++     LF+++FS+G SI+YRWAPM +K+ A+ TE  LL TFDYAWN   NG RR  
Sbjct: 133 SPVKAVTGGLFEDIFSKGGSILYRWAPMGSKREAEATEGMLLSTFDYAWNKGSNGERRQL 192

Query: 187 DVLKKLENIASQTTKSTFISRKLLPFTQKKMGIKIKTSKSIPMVNKPAE---DAEERNNF 246
           D+LKKL +    + + + ISR L PF + ++GI+IK  K +    +      D E+ NNF
Sbjct: 193 DLLKKLGDREFMSKRKSGISRMLFPFLRNQVGIRIKGEKHVLKEERKLTCDVDEEKSNNF 252

Query: 247 FSRILSFSRSRPRLV-DNTNDVNWADSN-SCGVVIGHNGEVCRKPPVEGRKRCAEHKGMK 306
            + IL  +RSRP+ V D  ++V+ + S+  CGV++  +G  C + PV+GRKRC EHKG +
Sbjct: 253 LTSILKLTRSRPQPVSDRFDEVDGSCSDIVCGVLL-EDGGCCIRSPVKGRKRCIEHKGQR 312

Query: 307 INGLLKNSSSRFILQKPVNVGTTIYGEKDFSCSKSEIPNSTEECSVSNSFPNKGSSLPIC 366
           +  +                          S  K   P S  E        N   S  +C
Sbjct: 313 VCRV--------------------------SPEKQTPPKS--EIFTGQDHHNHKDSDVVC 372

Query: 367 GVVLYDGSPCRRPPVQGRKRCEEHKGQRICRSTLVTSK--YQQTSPISKPESTVIAQGTS 426
           GV+L D  PC + PV GRKRCE+HKG RI     + ++   ++T    KP+     +  +
Sbjct: 373 GVILPDMEPCNKRPVPGRKRCEDHKGMRINAFLFLLNQTDREKTVKDEKPD----PESHT 431

Query: 427 SELSSSGLERMCGVDLGNGLNCTRQPVKGRVRCGEHK 453
             +    L R C     NGL CTR   KG  RC +HK
Sbjct: 433 ESIEEEALTRFCEATTKNGLPCTRSSPKGSKRCWQHK 431


HSP 2 Score: 92.0 bits (227), Expect = 1.0e-18
Identity = 72/189 (38.10%), Postives = 94/189 (49.74%), Query Frame = 1

Query: 268 CGVVIGHNGEVCRKPPVEGRKRCAEHKGMKINGLLKNSSSRFILQKPVNVGTTIYGEKDF 327
           CGV++  + E C K PV GRKRC +HKGM+IN  L      F+L +  +   T+  EK  
Sbjct: 338 CGVILP-DMEPCNKRPVPGRKRCEDHKGMRINAFL------FLLNQ-TDREKTVKDEKPD 397

Query: 328 SCSKSEIPNSTEECSVSNSFPNKGSSLPICGVVLYDGSPCRRPPVQGRKRCEEHKGQRIC 387
             S +E   S EE +++            C     +G PC R   +G KRC +HK +   
Sbjct: 398 PESHTE---SIEEEALTR----------FCEATTKNGLPCTRSSPKGSKRCWQHKEK--- 457

Query: 388 RSTLVTSKYQQTSPIS-KPESTVIAQGTSSELSSSGLERMCGVDLGNGLNCTRQPVKGRV 447
                TS    TSP+  +PE+   A+  +           CGV LGNGL C R PVKGR 
Sbjct: 458 -----TSS--DTSPVYFQPEA---AKNVA-----------CGVKLGNGLICERSPVKGRK 481

Query: 448 RCGEHKGMR 456
           RC EHKGMR
Sbjct: 518 RCEEHKGMR 481


HSP 3 Score: 84.0 bits (206), Expect = 2.8e-16
Identity = 48/113 (42.48%), Postives = 65/113 (57.52%), Query Frame = 1

Query: 356 ICGVVLYDGSPCRRPPVQGRKRCEEHKGQRICRSTLVTSKYQQTSPISKPESTVIAQGTS 415
           +CGV+L DG  C R PV+GRKRC EHKGQR+CR     S  +QT P S+     I  G  
Sbjct: 277 VCGVLLEDGGCCIRSPVKGRKRCIEHKGQRVCR----VSPEKQTPPKSE-----IFTGQD 336

Query: 416 SELSSSGLERMCGVDLGNGLNCTRQPVKGRVRCGEHKGMRTNKLISTLATSNK 469
              +    + +CGV L +   C ++PV GR RC +HKGMR N  +  L  +++
Sbjct: 337 HH-NHKDSDVVCGVILPDMEPCNKRPVPGRKRCEDHKGMRINAFLFLLNQTDR 379


HSP 4 Score: 48.1 bits (113), Expect = 1.7e-05
Identity = 21/36 (58.33%), Postives = 28/36 (77.78%), Query Frame = 1

Query: 263 ADSNSCGVVIGHNGEVCRKPPVEGRKRCAEHKGMKI 299
           A + +CGV +G NG +C + PV+GRKRC EHKGM+I
Sbjct: 448 AKNVACGVKLG-NGLICERSPVKGRKRCEEHKGMRI 482


HSP 5 Score: 44.3 bits (103), Expect = 2.5e-04
Identity = 23/55 (41.82%), Postives = 31/55 (56.36%), Query Frame = 1

Query: 417 ELSSSGLERMCGVDLGNGLNCTRQPVKGRVRCGEHKGMRTNKLISTLATSNKPDV 472
           E+  S  + +CGV L +G  C R PVKGR RC EHKG R  ++     T  K ++
Sbjct: 268 EVDGSCSDIVCGVLLEDGGCCIRSPVKGRKRCIEHKGQRVCRVSPEKQTPPKSEI 322

BLAST of Cucsa.397230 vs. TAIR10
Match: AT5G56770.1 (AT5G56770.1 BEST Arabidopsis thaliana protein match is: effector of transcription2 (TAIR:AT5G56780.1))

HSP 1 Score: 137.1 bits (344), Expect = 2.8e-32
Identity = 86/217 (39.63%), Postives = 115/217 (53.00%), Query Frame = 1

Query: 7   PIRLKREDCKRTKHDSDFSKWEILVASSDWEDYSLGKEGAERYRIHNLPKVSGPGLYELG 66
           P   KRED K T HD  FSKW  L+  +DW+D++  KE   RYR  +LP     GLYELG
Sbjct: 52  PTMFKREDYKLTIHDIAFSKWRNLIRHNDWKDFNNRKERVRRYRHEDLPPQRCTGLYELG 111

Query: 67  ITVSSSGLGREIAKLDADWIVV-VYLGEADNVRTRLQHYGRSGSHLGNAYFCVVDCKVFP 126
           + V     G+     D D  V+ VY+G+  +V++RLQ YGR G HL +            
Sbjct: 112 VGVIGQDQGQ---NFDPDNNVLGVYVGQCVDVKSRLQDYGRRGGHLPS------------ 171

Query: 127 LEKGPSLFQEMFSRGYSIVYRWAPMKNKKNAQMTETQLLKTFDYAWNTSGNGARRHDDVL 186
                 L++++FS GYS+ YRWAP      A  TE  LL TFDYAWNT  NG RRH    
Sbjct: 172 -----GLYEDIFSEGYSVFYRWAP-----EAAATEGMLLSTFDYAWNTCSNGERRH---- 231

Query: 187 KKLENIASQTTKSTFISRKLLPFTQKKMGIKIKTSKS 223
            +L+ +      S   S+ L+P  + ++ + IK  KS
Sbjct: 232 LELQKLGDPEFMSKRKSQVLVPSIRDQV-VTIKVEKS 238

BLAST of Cucsa.397230 vs. TAIR10
Match: AT4G26170.1 (AT4G26170.1 FUNCTIONS IN: molecular_function unknown)

HSP 1 Score: 111.3 bits (277), Expect = 1.7e-24
Identity = 83/262 (31.68%), Postives = 126/262 (48.09%), Query Frame = 1

Query: 201 ISRKLLPFTQKKMGIKIKTSKSIPMVNKP--AEDAEERNNFFSRILSFSRSRPRLVDNTN 260
           +S+ +LP TQ K   + +  KS     +   ++ AE+ +N    IL  SRSRP+ V   +
Sbjct: 234 LSQTILPTTQNKSDNQTEEKKSDSEEEREVSSDAAEKESNSLPSILRLSRSRPQPVSEKH 293

Query: 261 D--VNWADSNS-CGVVIGHNGEVCRKPPVEGRKRCAEHKGMKINGLLKNSSSRFILQKPV 320
           D  V+ +DS S CGV++  +G  C   PV+GRKRC EHKG +++               V
Sbjct: 294 DDIVDESDSASACGVLL-EDGTTCTTTPVKGRKRCTEHKGKRLSR--------------V 353

Query: 321 NVGTTIYGEKDFSCSKSEIPNSTEECSVSNSFPNKGSSLPICGVVLYDGSPCRRPPVQGR 380
           + G  I           E+P +  EC  + +         ICGV+L D   CR  PV  R
Sbjct: 354 SPGIHI---------PCEVP-TVRECEETEN---------ICGVILPDMIRCRSKPVSRR 413

Query: 381 KRCEEHKGQRICRSTLVTSKYQQTSPISKPESTVIAQGTSSELSSSGLERMCGVDLGNGL 440
           KRCE+HKG R+     + +  ++   +++ +S      TS+ ++  G   +C     NGL
Sbjct: 414 KRCEDHKGMRVNAFFFLLNPTERDKAVNEDKS---KPETSTGMNQEGSGLLCEATTKNGL 458

Query: 441 NCTRQPVKGRVRCGEHKGMRTN 458
            CTR   +G  RC +HK    N
Sbjct: 474 PCTRSAPEGSKRCWQHKDKTLN 458


HSP 2 Score: 84.3 bits (207), Expect = 2.2e-16
Identity = 61/190 (32.11%), Postives = 82/190 (43.16%), Query Frame = 1

Query: 266 NSCGVVIGHNGEVCRKPPVEGRKRCAEHKGMKINGLLKNSSSRFILQKPVNVGTTIYGEK 325
           N CGV++      CR  PV  RKRC +HKGM++N         F L  P      +  +K
Sbjct: 359 NICGVILPDMIR-CRSKPVSRRKRCEDHKGMRVNAF-------FFLLNPTERDKAVNEDK 418

Query: 326 DFSCSKSEIPNSTEECSVSNSFPNKGSSLPICGVVLYDGSPCRRPPVQGRKRCEEHKGQR 385
               SK E          S     +GS L +C     +G PC R   +G KRC +HK + 
Sbjct: 419 ----SKPE---------TSTGMNQEGSGL-LCEATTKNGLPCTRSAPEGSKRCWQHKDKT 478

Query: 386 ICRSTLVTSKYQQTSPISKPESTVIAQGTSSELSSSGLERMCGVDLGNGLNCTRQPVKGR 445
           +   +                S  +   T+S++       +CG  L NG  C + PVKGR
Sbjct: 479 LNHGS----------------SENVQSATASQV-------ICGFKLYNGSVCEKSPVKGR 503

Query: 446 VRCGEHKGMR 456
            RC EHKGMR
Sbjct: 539 KRCEEHKGMR 503

BLAST of Cucsa.397230 vs. NCBI nr
Match: gi|449435840|ref|XP_004135702.1| (PREDICTED: uncharacterized protein LOC101208130 [Cucumis sativus])

HSP 1 Score: 974.9 bits (2519), Expect = 5.0e-281
Identity = 478/478 (100.00%), Postives = 478/478 (100.00%), Query Frame = 1

Query: 1   MVAELSPIRLKREDCKRTKHDSDFSKWEILVASSDWEDYSLGKEGAERYRIHNLPKVSGP 60
           MVAELSPIRLKREDCKRTKHDSDFSKWEILVASSDWEDYSLGKEGAERYRIHNLPKVSGP
Sbjct: 1   MVAELSPIRLKREDCKRTKHDSDFSKWEILVASSDWEDYSLGKEGAERYRIHNLPKVSGP 60

Query: 61  GLYELGITVSSSGLGREIAKLDADWIVVVYLGEADNVRTRLQHYGRSGSHLGNAYFCVVD 120
           GLYELGITVSSSGLGREIAKLDADWIVVVYLGEADNVRTRLQHYGRSGSHLGNAYFCVVD
Sbjct: 61  GLYELGITVSSSGLGREIAKLDADWIVVVYLGEADNVRTRLQHYGRSGSHLGNAYFCVVD 120

Query: 121 CKVFPLEKGPSLFQEMFSRGYSIVYRWAPMKNKKNAQMTETQLLKTFDYAWNTSGNGARR 180
           CKVFPLEKGPSLFQEMFSRGYSIVYRWAPMKNKKNAQMTETQLLKTFDYAWNTSGNGARR
Sbjct: 121 CKVFPLEKGPSLFQEMFSRGYSIVYRWAPMKNKKNAQMTETQLLKTFDYAWNTSGNGARR 180

Query: 181 HDDVLKKLENIASQTTKSTFISRKLLPFTQKKMGIKIKTSKSIPMVNKPAEDAEERNNFF 240
           HDDVLKKLENIASQTTKSTFISRKLLPFTQKKMGIKIKTSKSIPMVNKPAEDAEERNNFF
Sbjct: 181 HDDVLKKLENIASQTTKSTFISRKLLPFTQKKMGIKIKTSKSIPMVNKPAEDAEERNNFF 240

Query: 241 SRILSFSRSRPRLVDNTNDVNWADSNSCGVVIGHNGEVCRKPPVEGRKRCAEHKGMKING 300
           SRILSFSRSRPRLVDNTNDVNWADSNSCGVVIGHNGEVCRKPPVEGRKRCAEHKGMKING
Sbjct: 241 SRILSFSRSRPRLVDNTNDVNWADSNSCGVVIGHNGEVCRKPPVEGRKRCAEHKGMKING 300

Query: 301 LLKNSSSRFILQKPVNVGTTIYGEKDFSCSKSEIPNSTEECSVSNSFPNKGSSLPICGVV 360
           LLKNSSSRFILQKPVNVGTTIYGEKDFSCSKSEIPNSTEECSVSNSFPNKGSSLPICGVV
Sbjct: 301 LLKNSSSRFILQKPVNVGTTIYGEKDFSCSKSEIPNSTEECSVSNSFPNKGSSLPICGVV 360

Query: 361 LYDGSPCRRPPVQGRKRCEEHKGQRICRSTLVTSKYQQTSPISKPESTVIAQGTSSELSS 420
           LYDGSPCRRPPVQGRKRCEEHKGQRICRSTLVTSKYQQTSPISKPESTVIAQGTSSELSS
Sbjct: 361 LYDGSPCRRPPVQGRKRCEEHKGQRICRSTLVTSKYQQTSPISKPESTVIAQGTSSELSS 420

Query: 421 SGLERMCGVDLGNGLNCTRQPVKGRVRCGEHKGMRTNKLISTLATSNKPDVSDTGSVS 479
           SGLERMCGVDLGNGLNCTRQPVKGRVRCGEHKGMRTNKLISTLATSNKPDVSDTGSVS
Sbjct: 421 SGLERMCGVDLGNGLNCTRQPVKGRVRCGEHKGMRTNKLISTLATSNKPDVSDTGSVS 478

BLAST of Cucsa.397230 vs. NCBI nr
Match: gi|659099909|ref|XP_008450835.1| (PREDICTED: uncharacterized protein LOC103492307 [Cucumis melo])

HSP 1 Score: 901.7 bits (2329), Expect = 5.3e-259
Identity = 447/478 (93.51%), Postives = 459/478 (96.03%), Query Frame = 1

Query: 1   MVAELSPIRLKREDCKRTKHDSDFSKWEILVASSDWEDYSLGKEGAERYRIHNLPKVSGP 60
           MVAELS IRLKREDCKRTKHDS FSKWEILVASSDWEDYSLGKEGAERYRIHNLPKVSGP
Sbjct: 1   MVAELSQIRLKREDCKRTKHDSSFSKWEILVASSDWEDYSLGKEGAERYRIHNLPKVSGP 60

Query: 61  GLYELGITVSSSGLGREIAKLDADWIVVVYLGEADNVRTRLQHYGRSGSHLGNAYFCVVD 120
           GLYELGITVSSSGLGREIAKLDAD IVVVYLGEADNVRTRLQHYGRSGSHLGN Y  V D
Sbjct: 61  GLYELGITVSSSGLGREIAKLDADCIVVVYLGEADNVRTRLQHYGRSGSHLGNGYLSVED 120

Query: 121 CKVFPLEKGPSLFQEMFSRGYSIVYRWAPMKNKKNAQMTETQLLKTFDYAWNTSGNGARR 180
           CKV PLEKGPSLFQEMFSRGYSIVYRWAPMKNK++AQMTE QLLKTFDYAWNTSGNGARR
Sbjct: 121 CKVVPLEKGPSLFQEMFSRGYSIVYRWAPMKNKRDAQMTEAQLLKTFDYAWNTSGNGARR 180

Query: 181 HDDVLKKLENIASQTTKSTFISRKLLPFTQKKMGIKIKTSKSIPMVNKPAEDAEERNNFF 240
           H+DV+KKLE+IASQTTKSTFISRKLLPF QKKMGIKIKTSK IP+VNKPAEDAEERNNFF
Sbjct: 181 HNDVIKKLEDIASQTTKSTFISRKLLPFRQKKMGIKIKTSKPIPIVNKPAEDAEERNNFF 240

Query: 241 SRILSFSRSRPRLVDNTNDVNWADSNSCGVVIGHNGEVCRKPPVEGRKRCAEHKGMKING 300
           SRILSFSRSRPRLVDNTNDVNWADSNSCGVVIGH GEVCRKPPVEGRKRCAEHKGMKING
Sbjct: 241 SRILSFSRSRPRLVDNTNDVNWADSNSCGVVIGH-GEVCRKPPVEGRKRCAEHKGMKING 300

Query: 301 LLKNSSSRFILQKPVNVGTTIYGEKDFSCSKSEIPNSTEECSVSNSFPNKGSSLPICGVV 360
           LLKNSSSR  LQKPVNVGTTIYGEKDFSCSKSEIPN+TEECSVSNSF N+GSSLPICGVV
Sbjct: 301 LLKNSSSRLNLQKPVNVGTTIYGEKDFSCSKSEIPNNTEECSVSNSFTNEGSSLPICGVV 360

Query: 361 LYDGSPCRRPPVQGRKRCEEHKGQRICRSTLVTSKYQQTSPISKPESTVIAQGTSSELSS 420
           LYDGSPCRRPPVQGRKRCEEHKG+RICRSTLVTSKY+QTSPISKPESTVIA+GTSSELSS
Sbjct: 361 LYDGSPCRRPPVQGRKRCEEHKGKRICRSTLVTSKYKQTSPISKPESTVIARGTSSELSS 420

Query: 421 SGLERMCGVDLGNGLNCTRQPVKGRVRCGEHKGMRTNKLISTLATSNKPDVSDTGSVS 479
           SGLERMCGVDLGNGL CTRQPVKGRVRCGEHKGMRTNKLI  LATS KPDVSDTGSV+
Sbjct: 421 SGLERMCGVDLGNGLYCTRQPVKGRVRCGEHKGMRTNKLILRLATSIKPDVSDTGSVN 477

BLAST of Cucsa.397230 vs. NCBI nr
Match: gi|703075246|ref|XP_010090025.1| (hypothetical protein L484_027255 [Morus notabilis])

HSP 1 Score: 460.3 bits (1183), Expect = 4.1e-126
Identity = 246/476 (51.68%), Postives = 321/476 (67.44%), Query Frame = 1

Query: 9   RLKREDCKRTKHDSDFSKWEILVASSDWEDYSLGKEGAERYRIHNLPKVSGPGLYELGIT 68
           RLKREDCKRTKHD +FSKWEIL+  SDWE+Y LGKEGAERYR+HNLPK SGPG+YELGI 
Sbjct: 63  RLKREDCKRTKHDREFSKWEILIGPSDWEEYLLGKEGAERYRVHNLPKNSGPGVYELGIA 122

Query: 69  VSSSGLGREIAKLDADWIVVVYLGEADNVRTRLQHYGRSGSHLGNAYFCVVDCKVFPLEK 128
           VS +GLGREI KLD + IVVVYLG+ADNVRTRLQHYGR+G+HLGN+  C   CK    +K
Sbjct: 123 VSRTGLGREIGKLDPERIVVVYLGQADNVRTRLQHYGRTGAHLGNS--CPPQCKTVIPQK 182

Query: 129 GPSLFQEMFSRGYSIVYRWAPMKNKKNAQMTETQLLKTFDYAWNTSGNGARRHDDVLKKL 188
           GP LF+E+ SRGY I++RWAPM++K +A  TET+LL TFDYAWNTS NG RR DD+L KL
Sbjct: 183 GPGLFEEILSRGYPIIFRWAPMQSKSDALKTETELLNTFDYAWNTSINGTRRPDDILNKL 242

Query: 189 ENIASQTTKSTFISRKLLPFTQKKMGIKIKTSKSIPMVNKPAEDA-EERNNFFSRILSFS 248
           + I+S     + I+RKL+PF+QK++GI+IK  K      K    A EE  N FSR+  F 
Sbjct: 243 KKISSSNIHFSDIARKLVPFSQKQVGIRIKARKFPSAEEKIDTSADEESQNIFSRVFKFG 302

Query: 249 RSRPRLVDNTNDVNWADSNSCGVVIGHNGEVCRKPPVEGRKRCAEHKGMKINGLLKNSSS 308
           RS+P+LV + + +   D+  CG+V+G +  +CR+PPVEGRKRCAEHKGM+I G       
Sbjct: 303 RSQPKLVLDRSVIVLEDTTICGMVLGLD-SICRRPPVEGRKRCAEHKGMRIKG------- 362

Query: 309 RFILQKPVNV---GTTIYGEKDFSCSKSEIPNSTEECSVSNSFPNKGSSLPICGVVLYDG 368
                 PV+V    + +  +  FS   S + +S E   V+ +  ++ S  PICG +L DG
Sbjct: 363 ------PVSVALSNSNVNSQSTFSAPSSSL-HSEELQPVTFNNASRESFTPICGFILADG 422

Query: 369 SPCRRPPVQGRKRCEEHKGQRICRSTLVTS-----KYQQTSPISKPESTVI-------AQ 428
           SPC   P++G KRC EHKG++I + T   S      Y    P+S+ +S+ I       AQ
Sbjct: 423 SPCISQPLKGNKRCLEHKGRKILQQTNSASVTKEKLYYLPDPVSESKSSEICFSGEVQAQ 482

Query: 429 GTSSELSSSGLERMCGVDLGNGLNCTRQPVKGRVRCGEHKGMRTNKLISTLATSNK 469
             +  L+++  + +CGV LG+GL CTR+PV GRVRC EHKG+R  +  +T   + K
Sbjct: 483 AFAKRLNAN-CDTICGVVLGDGLVCTRKPVVGRVRCEEHKGIRVTRFATTKFAAEK 520

BLAST of Cucsa.397230 vs. NCBI nr
Match: gi|703075246|ref|XP_010090025.1| (hypothetical protein L484_027255 [Morus notabilis])

HSP 1 Score: 43.5 bits (101), Expect = 1.2e+00
Identity = 21/51 (41.18%), Postives = 29/51 (56.86%), Query Frame = 1

Query: 426 MCGVDLGNGLNCTRQPVKGRVRCGEHKGMRTNKLISTLATSNKPDVSDTGS 477
           +CG+ LG    C R PV+GR RC EHKGMR    +S   +++  +   T S
Sbjct: 320 ICGMVLGLDSICRRPPVEGRKRCAEHKGMRIKGPVSVALSNSNVNSQSTFS 370


HSP 2 Score: 422.9 bits (1086), Expect = 7.3e-115
Identity = 248/493 (50.30%), Postives = 314/493 (63.69%), Query Frame = 1

Query: 1   MVAELSPI--RLKREDCKRTKHDSDFSKWEILVASSDWEDYSLGKEGAERYRIHNLPKVS 60
           MV    PI  RLKREDCKRTKHDS+F+KW+ILV  SDWED+S  KEGAERYR+HNLP   
Sbjct: 1   MVTTEPPIVSRLKREDCKRTKHDSEFTKWKILVGPSDWEDHSSRKEGAERYRVHNLPLCC 60

Query: 61  G-PGLYELGITVSSSGLGREIAKLDADWIVVVYLGEADNVRTRLQHYGRSGSHL------ 120
             PG+YELGI+VS +GLGRE+ KLD   IVVVYLG+A+++R RLQHYGRSG+HL      
Sbjct: 61  HCPGVYELGISVSPTGLGREVGKLDPAHIVVVYLGQAEDIRARLQHYGRSGAHLAKVDST 120

Query: 121 GNAYFCVVDCKVFPLEKGPSLFQEMFSRGYSIVYRWAPMKNKKNAQMTETQLLKTFDYAW 180
           GN      +CK  PL++G  LF+ +FSRGYSIV+RWAP+++K++A+ TE++LLKTFDYAW
Sbjct: 121 GNPN----NCKNMPLQRGSGLFEGIFSRGYSIVFRWAPIESKRDAERTESRLLKTFDYAW 180

Query: 181 NTSGNGARRHDDVLKKLENIASQTTKSTFISRKLLPFTQKKMGIKIKTSKSIPMVNKPAE 240
           N   NGARR  DVL+KLE  +S T     I+RK     QK++GI IK SK     N  + 
Sbjct: 181 NKGSNGARRPSDVLRKLEKTSSSTPPFPSITRKFQMLGQKQVGIIIKASKPFLQENGLST 240

Query: 241 DAEERNNFFSRILSFSRSRPRLVDNTNDVNWADSNSCGVVIGHNGEVCRKPPVEGRKRCA 300
           D ++ N+  +R+  F RS+PRLV +   V    +++CGV +G +G +C +PPVEGRKRCA
Sbjct: 241 D-QDINSLLARVFKFGRSQPRLVSDRVGVTGNYNSTCGVALG-DGTICERPPVEGRKRCA 300

Query: 301 EHKGMKINGLLKNSSSRFILQ-KPVNVGTTIYGEKDFSCSKSEIPNST---EECSVSNSF 360
           EHKGMK     K+S S+ I++ KP  V      E   + S     N      +C V+  F
Sbjct: 301 EHKGMK----TKSSFSKPIVERKPQMVDLNRESEGHSAQSYGYTVNFQLVGGKCPVNKDF 360

Query: 361 PNKGSSLPICGVVLYDGSPCRRPPVQGRKRCEEHKGQR----ICRSTLVTSKYQQTSPIS 420
                   ICGV+L DG PC+R PV GRKRCEEHKG R    I  ST+  S +      S
Sbjct: 361 ------TIICGVILDDGFPCKRQPVLGRKRCEEHKGMRTNVSISNSTVGKSHHTYE---S 420

Query: 421 KPESTVIAQGTSSELSSSGLERMCGVDLGNGLNCTRQPVKGRVRCGEHKGMRTNKLISTL 477
           K +  V+    S   +  G+   CGVDLG+G+ C R  V GR RC EHKGMR N LISTL
Sbjct: 421 KLQPQVLPDCRSFSNTDYGI--TCGVDLGHGIFCKRLAVAGRKRCEEHKGMRINSLISTL 472

BLAST of Cucsa.397230 vs. NCBI nr
Match: gi|731406599|ref|XP_010656218.1| (PREDICTED: uncharacterized protein LOC100264589 [Vitis vinifera])

HSP 1 Score: 108.6 bits (270), Expect = 3.0e-20
Identity = 85/246 (34.55%), Postives = 114/246 (46.34%), Query Frame = 1

Query: 214 GIKIKTSKSIPMVNKPAE--DAEERNNFFSRILSFSRSRPRLVDNTNDVNWADSNSCGVV 273
           G+K K+S S P+V +  +  D    +   S          +LV     VN   +  CGV+
Sbjct: 298 GMKTKSSFSKPIVERKPQMVDLNRESEGHSAQSYGYTVNFQLVGGKCPVNKDFTIICGVI 357

Query: 274 IGHNGEVCRKPPVEGRKRCAEHKGMKINGLLKNSSSRFILQKPVNVGTTIYGEKDFSCSK 333
           +  +G  C++ PV GRKRC EHKGM+ N  + NS+                G+   +   
Sbjct: 358 LD-DGFPCKRQPVLGRKRCEEHKGMRTNVSISNST---------------VGKSHHTYES 417

Query: 334 SEIPNSTEECSVSNSFPNKGSSLPICGVVLYDGSPCRRPPVQGRKRCEEHKGQRICRSTL 393
              P    +C    SF N    +  CGV L  G  C+R  V GRKRCEEHKG RI  S +
Sbjct: 418 KLQPQVLPDC---RSFSNTDYGIT-CGVDLGHGIFCKRLAVAGRKRCEEHKGMRI-NSLI 477

Query: 394 VTSKYQQTSPISKPESTVIAQGTSSELSSSGLERMCGVDLGNGLNCTRQPVKGRVRCGEH 453
            T   +  S      S  I    S+   +S     CG+ LG+G  C RQPV+G  RC +H
Sbjct: 478 STLAGEDKSHALDMGSGFITYNNSNYNHTS----TCGMTLGDGSICKRQPVEGNKRCWQH 518

Query: 454 KGMRTN 458
           KGMR N
Sbjct: 538 KGMRVN 518


HSP 2 Score: 99.0 bits (245), Expect = 2.4e-17
Identity = 72/211 (34.12%), Postives = 102/211 (48.34%), Query Frame = 1

Query: 247 SRSRPRLVDNTNDVNWADSN-SCGVVIGHNGEVCRKPPVEGRKRCAEHKGMKINGLLKNS 306
           S+ +P+++ +    +  D   +CGV +GH G  C++  V GRKRC EHKGM+IN L+   
Sbjct: 401 SKLQPQVLPDCRSFSNTDYGITCGVDLGH-GIFCKRLAVAGRKRCEEHKGMRINSLI--- 460

Query: 307 SSRFILQKPVNVGTTIYGEKDFSCSKSEIPNSTEECSVSNSFPNKGSSLPICGVVLYDGS 366
                        +T+ GE         +   +   + +NS  N  S+   CG+ L DGS
Sbjct: 461 -------------STLAGED----KSHALDMGSGFITYNNSNYNHTST---CGMTLGDGS 520

Query: 367 PCRRPPVQGRKRCEEHKGQRI-CRSTLVTSKYQQTSPISKPESTVIAQGTSSELSSSGLE 426
            C+R PV+G KRC +HKG R+ C S+   S           E+T                
Sbjct: 521 ICKRQPVEGNKRCWQHKGMRVNCSSSWFGS-----------ETT---------------- 559

Query: 427 RMCGVDLGNGLNCTRQPVKGRVRCGEHKGMR 456
            +CGV L NG  C+R P  GR RC +HKGMR
Sbjct: 581 -LCGVLLRNGSVCSRVPAHGRKRCEQHKGMR 559


HSP 3 Score: 415.2 bits (1066), Expect = 1.5e-112
Identity = 232/477 (48.64%), Postives = 293/477 (61.43%), Query Frame = 1

Query: 9   RLKREDCKRTKHDSDFSKWEILVASSDWEDYSLGKEGAERYRIHNLPKVSGPGLYELGIT 68
           RLKREDCKRTKHD  FS+W+ILV   DWEDYSLGK+GAERYR+HNLPK+S PGLYEL I 
Sbjct: 24  RLKREDCKRTKHDLVFSQWKILVGPCDWEDYSLGKDGAERYRVHNLPKISTPGLYELAIA 83

Query: 69  VSSSGLGREIAKLDADWIVVVYLGEADNVRTRLQHYGRSGSHLGNAYFCVVDCKVFPLEK 128
           VS +GLGR IAKLD + IVVVYLG A+NVRTRLQ YGR+GSHLG    C        L  
Sbjct: 84  VSHTGLGRNIAKLDPERIVVVYLGHAENVRTRLQRYGRTGSHLGTC--CSTAHPTLSLHN 143

Query: 129 GPSLFQEMFSRGYSIVYRWAPMKNKKNAQMTETQLLKTFDYAWNTSGNGARRHDDVLKKL 188
           GP LF ++ SRGY IV+RWAPM++K +AQ TET LL TFDYAWNTS NG+RR D++L+KL
Sbjct: 144 GPGLFDDILSRGYPIVFRWAPMQSKSDAQKTETLLLNTFDYAWNTSINGSRRPDEILEKL 203

Query: 189 ENIASQTTKSTFISRKLLPFTQKKMGIKIKTSKSIPMVN--KPAEDAEERNNFFSRILSF 248
           + I+S + +   + R+LLP +Q+++GI+IK  K +   N   P  D EER N  SR+  F
Sbjct: 204 KKISSSSNRFPAVVRRLLPLSQRQVGIRIKAGKLLATENNSNPCAD-EERPNILSRVFKF 263

Query: 249 SRSRPRLVDNTNDVNWADSNSCGVVIGHNGEVCRKPPVEGRKRCAEHKGMKINGL----L 308
            RSRPR V +T+ +   ++  CGV + H+  VC + PVEGRKRCAEHKG+KINGL    +
Sbjct: 264 GRSRPRSVLDTSGITQGNTVICGVALTHD-SVCTRAPVEGRKRCAEHKGVKINGLITVRI 323

Query: 309 KNSSSRFILQKPVNVGTTIYGEKDFSCSKSEIPNSTEECSVSNSFPNKGSSLPICGVVLY 368
            NS+S +  Q         YG  D + S     +     +V+  F    S  PICG  L 
Sbjct: 324 SNSNSIYGSQ---------YGVIDSTKSDGHGKSGFRNDTVTVEFHGSKSLGPICGFTLT 383

Query: 369 DGSPCRRPPVQGRKRCEEHKGQRICRST---------------LVTSKYQQTSPISKPES 428
           DGSPCRR  V G KRC+EHKG+RI +S                +  S    TS      +
Sbjct: 384 DGSPCRRQAVPGNKRCDEHKGRRIRKSNSRLVSTEEKPLYVPDVFESNCGDTSEYDNNSA 443

Query: 429 TVIAQG-TSSELSSSGLER--------MCGVDLGNGLNCTRQPVKGRVRCGEHKGMR 456
            +   G    E+ SS + +         CG    NG  C R+ V+G  RC +H+GMR
Sbjct: 444 AICNHGRLEDEVLSSRINQNKNGIGVVTCGATTKNGSACKRKQVEGNKRCWQHRGMR 487

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ET2_ARATH4.3e-8340.70Protein EFFECTOR OF TRANSCRIPTION 2 OS=Arabidopsis thaliana GN=ET2 PE=1 SV=1[more]
ET3_ARATH5.0e-3139.63Protein EFFECTOR OF TRANSCRIPTION 3 OS=Arabidopsis thaliana GN=ET3 PE=4 SV=1[more]
ET1_ARATH2.9e-2331.68Protein EFFECTOR OF TRANSCRIPTION 1 OS=Arabidopsis thaliana GN=ET1 PE=2 SV=1[more]
ET_BRANA2.0e-1634.04Protein EFFECTOR OF TRANSCRIPTION OS=Brassica napus GN=ET PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LWE7_CUCSA3.5e-281100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G586820 PE=4 SV=1[more]
W9R5X1_9ROSA2.9e-12651.68Uncharacterized protein OS=Morus notabilis GN=L484_027255 PE=4 SV=1[more]
W9R5X1_9ROSA8.4e-0141.18Uncharacterized protein OS=Morus notabilis GN=L484_027255 PE=4 SV=1[more]
D7TB99_VITVI2.1e-2034.55Putative uncharacterized protein OS=Vitis vinifera GN=VIT_11s0016g02440 PE=4 SV=... [more]
A0A061GKU1_THECC5.8e-1032.16Effector of transcription2, putative OS=Theobroma cacao GN=TCM_037691 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G56780.12.4e-8440.70 effector of transcription2[more]
AT5G56770.12.8e-3239.63 BEST Arabidopsis thaliana protein match is: effector of transcriptio... [more]
AT4G26170.11.7e-2431.68 FUNCTIONS IN: molecular_function unknown[more]
Match NameE-valueIdentityDescription
gi|449435840|ref|XP_004135702.1|5.0e-281100.00PREDICTED: uncharacterized protein LOC101208130 [Cucumis sativus][more]
gi|659099909|ref|XP_008450835.1|5.3e-25993.51PREDICTED: uncharacterized protein LOC103492307 [Cucumis melo][more]
gi|703075246|ref|XP_010090025.1|4.1e-12651.68hypothetical protein L484_027255 [Morus notabilis][more]
gi|703075246|ref|XP_010090025.1|1.2e+0041.18hypothetical protein L484_027255 [Morus notabilis][more]
gi|731406599|ref|XP_010656218.1|3.0e-2034.55PREDICTED: uncharacterized protein LOC100264589 [Vitis vinifera][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
molecular_function GO:0003677 DNA binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.397230.1Cucsa.397230.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR35133FAMILY NOT NAMEDcoord: 7..467
score: 1.2E