Cucsa.394150 (gene) Cucumber (Gy14) v1

NameCucsa.394150
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionL-ascorbate oxidase
Locationscaffold04100 : 202468 .. 207346 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCCTCCTCTCTCCTCTTCCTCTTCATTTCTTTCTTCTTCGCCATTGTCCGAGCTGAGGATCCTTACCGCTTCTTCGACTGGAATGTAACTTACGGCACCATTTACCCCCTCGGCCTCCCCCAGCAGGTTTGCTTGTTGCCTTTTCTCTTCATTTTCCATACTCATTCTCTCTCTCTTTCTTCCTCCATTTCTTAACGGATCCGAATCTCCTCTCCTCTTCTTTCTTCTTAGGGCATTTTGATTAATGGCCAATTTCCTGGCCCCGATATTCACTCTGTTACCAACGACAATATCATTATCAATGTTTTCAATAGCTTGGATGAGCCTTTTCTCCTCTCCTGGTAATTTCTCCCTCCCTCGCTTTTACTCGACATCTCAAATTTAGTTCATGAATATGGAAATGTGCTTTCTAGAATGCGCTGTGTTTATTCGTTAGATTTGGAGTTGAAATTTCTGATTTCTGGAGTTAATTTGGTGAAATTTGGGGCTTGGATCTGGTTTTAACTTGTTGAATAGTGTGTTTGGGACAATGCCTGTAAATTATCGTTGTTATTGGATTTAGTGTCTAAATTTAGGGGAAGTGTTATGTAATTAGGAATGGGATTCAACAGAGAAGGAACTCGTATGAAGATGGTGTGTACGGAACAACTTGCCCCATTCCTCCAGGGAAGAACTTCACTTACATTCTTCAAATGAAAGATCAGATCGGAAGTTTCTATTACTTCCCTTCTCTTGGCTTCCATAAGGCCGCCGGTGGGTTTGGTGGAATCAGAATTCTTAGCCGTCCTCGGATTCCTGTTCCGTTCCCGGATCCTGCCGGGGATTATACAGTCCTTATCGGTGACTGGTACCAAGCTAATCATACGGTAAATAATCACTAATTAATTATCCCTTTTTTGCTTTCAACAACTTTTCAAAATTAAGCAATTGTTACTGCTTTTTTTATAAAGGGGAGATTCGATTGAACTTTTAATCAATCATATTAGTTTCGAAATCGAGCTCTGGCTAATGTGGAAAGGTGAAAGAATACTGAATAATGATAGTCACGGAGCCAGATTGGCGGGGAATAGGGAATCACTTTTTCTTTATATATATAAATATTTAAAACTTTCGCTATATATATTACCTTACAATGAATAGACTTTTCCTTCTGTCTTGATAAAAACAACATGAAGTGGTAATAGCAACTAATATACTTGTTATAGATTAAGATTGAAAAAGAACTTTTAATACATCTCCTCCATTCGGAAATTTTCTATTTCAATGGGAGTTTTTAACACACACACACAAAAACAATATTATAATTACTAGAATTTATCAGCACTAAACTTTATTTTATAAAGAATGATATTTTGATAGAAAAAATAAATTTTTTTTGATAAGTTTTGTTATAATTTGTAAATATTTTGATTTATTTTTTATATTTAAAAATAAAAAAAATTTGCATGTATGAAGAGTACATAATTTATCACGTACAGAATTGAGCTCATGAGTGTGTTCTAGATCCCAAATGTTTTTCTTATGTTTTTTTTTCTTCTTTCTTTTTACCTCTGTCGGTAGACGTAGACCATTGGTGGTTCTAATGCTTGACCCATAATCTTTGACTCTTCAAATCCTTTTTGCTTCTCTCTCTAGTCTCTCTCTCTCTCCCGAGATTAACAGCTGGATTTAATTAAAAGGGTGCTTTGTTTCTTTAATGGTGCGGGCCATGGTTGCCTCGACTGTCAGTGCGATAATCATAAGCTGAATTTAATTGACTTTTTGGTAATGGCATGTGCCTCTATTTGATAAAAGAAAAGCCTTTTTCATTGTGTTTCTTTTTCCCCCTATTTTGAAAGGAGCTTTTTTTGTATTGTTGGGTTGTATGGAATCCAGAATCCAGCTAACGAAAGCGCTTTTACGAATCAGTTTTAGGCACTGAATCCCAGGCTCTCCCAAACGCGCTTTCTTCCAACTTAAATCCTCTCATTAATTTAACCCTATGAAACTCCCCTTTATTAGCAAAAAGACAACTATATTTATCCCCTACCACTACCTAATCCTTTCCTTCATTATTATGGTCTTTTCTTCTTCTCGCTTTATATTTACCAATTCCTTTTGAAAGAAGGGTCCTTTTTCTTTTTTAGAAAAGATCATTTGTAACTCTTATAAAATACTATATGACAAAAAGTTTGTGTAAAGCCACCCTTTTTCGTTTGGTTCTTGCCTAAGTTTTTAGCCCACTGTTTTTGGAATGGTTATAGCTGATCAGTTGACATAAAATATTTGTTATCAACCTTGGTGCTTTAGATTTAATCCCTTCCCTCTCATGCTGAATTGAAAAAAAAATGTTAAAATTACCATTTTTATTCTTATATTAAGCAGCTGACAATTTTTATTGTGACTAAACAATAATAATTTTGACGGAAATTTTTATTAACTAGAAGAAGAAATTAAATTTAAGATTTATGAAAAATAGAAGACTAGAATTGAATAAACCAAAAGTAAATTAACAAAATAGAATTCCAACCTAATTTTTATACTACCTGAATTCCACCCACCAATTACTCGAAAAAAAACTCAACCACCCATTCTCCAAATAGTCCAATTGATAACGGAAGAAGAAACAAAAGTGCCCTCCTTTTCAGTCTTGCTTACTTTTCTGTTTTTGCTTATTATTCACTTTTAATGAGGGGGTCGTTATTACTAGGATTGGCAATATGAGAAACTGTTAACTTGTATCTGTTTTTAAGTAAGTGATTCCATCTCAAGTAAAAGTAGAAACACACGATTTATATGTGTAGAAACATAATCTTCTTACATAACTAAATATGGATCTGACATCTGAGCATTATTCCTTTCCCTTCAAATAATTTAGCCATTTTTAGTATACAAATTAATTGAATTTGTTTTTGCAGACGTTGAGGGCCCAATTAGACAATGGCTCAATGCTTCCTCTCCCCGATGGAATCCTCATCAATGGTCGTGGACCCAATAGAACTGCCTCAATCAACGTGGAACAAGGTGATAATGAGAGAAACGCACTACTCAATATTTTTTAAATCAATCCACTTTGTATCTAGTAGTTATTAAGAATAGAGACTCGTTTACGACCTTACTTGTACATGATATGTTCTAGTGTCGGAGACTCATCATTTGTGTGTATATGTGTTATTAGGAAAAACTTACAGGCTGAGGATTTCAAACGTGGGGCTTCAAAGTTCTCTGAACTTCCGAATTCAAGGCCACAGAATGAAGGTGGTGGAAGTTGAAGGAACCCATACCCTCCAAACGGAATTCTCTTCCCTTGACATTCACGTAGGCCAATCTTATTCCGTCCTTGTCACTGCGGATCAGCCCGCTCAGGACTTTTACATTGTGGTTTCTTCTCGCTTTACTACTCCCATCCTCTCCACCACCGCTATTCTCCGTTATGCCAACTCCGCCGGCTCCGTCCAAGGCCCGCCTCCTGGTGGCCCAACTATCCAAATCGATTGGTCCCTTAATCAAGCTCGAGCCATCAGGTAATCCTGCCTAACTTTACTTTAACTACTACATCAGTTTCATAAACCCTTTATTGTTTCTTTAAAAAATTGCTTTTCGTTCAATAATGTGAAGAAATTGGCATACGAATGCAGAACCAATCTTACAGCCAGTGGGCCACGGCCGAACCCACAAGGGTCGTATCACTATGGTATGATAACCACGACCAGAACCATTGTAGTGGCGAACTCAGCCGGAATTGTGGACGGAAAGCAAAGATACGCCGTGAACAGCGTGTCATTTATTCCGGCAGACACTCCGCTGAAGGTGGCAGACTTCTTCAAAATAGGAGGAGTTTTCCGGGTGGGAAGCATTTCCGACTGGCCAAACGGTGGAGGAATCTATCAAGACACCTCTGTTATGGGCGCCGATTACAGAGCTTTTGTCGAAATCGTTTTTCAGAACAACGAAGACCTCATTCAGAGCTGGCATCTTGATGGCTACTCCTTCTTCGTTGTTGGGTAATTAACATTCTTTTACGATTCTTCTTCTTCTTCTTTTTTAATTTTTTTTAATGAAAACAAATGGTTGTGAATTTCAGTATGGATGGAGGGCAATGGACATCGAATAGCAGAAACCAATACAATCTACGAGACGCAATTGCACGGTGTACTGTTCAGGTACAACCCAAACTCCTGTTTTTCTTTTGTTTCTATTTGATTACGTTTTTTCTTCTAAAAAACAAATTATTTGTTCTTTTACTGGTCTTCAATAAACAATTAACCGGGCCTAATCAAATTTCATAATTGAAAACAAATGTTTCAATGTAAGAAGACGGTGAGAAAACAAACATTTGTTTTAAAAAAAAAACAAAAGAAAATTGTCTAAAGACAGAAATGAATTATTGTTAATCTTGGCAGGTGTATCCAAAGTCATGGAGTGCGATATATGTGGCGCTAGACAACGTTGGAATGTGGAATATAAGGACAGAATTTTGGGCAAGGCAATACTTAGGACAACAATTTTACTTAAGAGTTTACACTCCATCAACGTCATTAAGAGATGAGTACCCAATTCCAAAGAATGCTCTTCTTTGTGGAAGAGCCACCGGCAGAAGAACCAGGCCACTCTAATTTCCCCATTTATCATCATATCATTTACATAATCTTCTTCTTCCAGCTCGTAAAGAAAAAAAGAAAAAACCATTAATCATTATTGCTTCTCAAAGTTAAAATGTCCTTAGCTCGAGGTCATTTATTTATATATTTTTTTCTTTAATTTCTATTCATAGCATTTCATTGAATCGAATGTCCTTTCTTGATCTTCTTGCACTTGCAGAAGTCATTTTTTTTCTCTCTGTTTAGGTCATAAATATTCTTATGTTTGAGATAATTAAAGGCTCAATATATTAACA

mRNA sequence

ATGCCCTCCTCTCTCCTCTTCCTCTTCATTTCTTTCTTCTTCGCCATTGTCCGAGCTGAGGATCCTTACCGCTTCTTCGACTGGAATGTAACTTACGGCACCATTTACCCCCTCGGCCTCCCCCAGCAGGGCATTTTGATTAATGGCCAATTTCCTGGCCCCGATATTCACTCTGTTACCAACGACAATATCATTATCAATGTTTTCAATAGCTTGGATGAGCCTTTTCTCCTCTCCTGGAATGGGATTCAACAGAGAAGGAACTCGTATGAAGATGGTGTGTACGGAACAACTTGCCCCATTCCTCCAGGGAAGAACTTCACTTACATTCTTCAAATGAAAGATCAGATCGGAAGTTTCTATTACTTCCCTTCTCTTGGCTTCCATAAGGCCGCCGGTGGGTTTGGTGGAATCAGAATTCTTAGCCGTCCTCGGATTCCTGTTCCGTTCCCGGATCCTGCCGGGGATTATACAGTCCTTATCGGTGACTGGTACCAAGCTAATCATACGACGTTGAGGGCCCAATTAGACAATGGCTCAATGCTTCCTCTCCCCGATGGAATCCTCATCAATGGTCGTGGACCCAATAGAACTGCCTCAATCAACGTGGAACAAGGAAAAACTTACAGGCTGAGGATTTCAAACGTGGGGCTTCAAAGTTCTCTGAACTTCCGAATTCAAGGCCACAGAATGAAGGTGGTGGAAGTTGAAGGAACCCATACCCTCCAAACGGAATTCTCTTCCCTTGACATTCACGTAGGCCAATCTTATTCCGTCCTTGTCACTGCGGATCAGCCCGCTCAGGACTTTTACATTGTGGTTTCTTCTCGCTTTACTACTCCCATCCTCTCCACCACCGCTATTCTCCGTTATGCCAACTCCGCCGGCTCCGTCCAAGGCCCGCCTCCTGGTGGCCCAACTATCCAAATCGATTGGTCCCTTAATCAAGCTCGAGCCATCAGAACCAATCTTACAGCCAGTGGGCCACGGCCGAACCCACAAGGGTCGTATCACTATGGTATGATAACCACGACCAGAACCATTGTAGTGGCGAACTCAGCCGGAATTGTGGACGGAAAGCAAAGATACGCCGTGAACAGCGTGTCATTTATTCCGGCAGACACTCCGCTGAAGGTGGCAGACTTCTTCAAAATAGGAGGAGTTTTCCGGGTGGGAAGCATTTCCGACTGGCCAAACGGTGGAGGAATCTATCAAGACACCTCTGTTATGGGCGCCGATTACAGAGCTTTTGTCGAAATCGTTTTTCAGAACAACGAAGACCTCATTCAGAGCTGGCATCTTGATGGCTACTCCTTCTTCGTTGTTGGTATGGATGGAGGGCAATGGACATCGAATAGCAGAAACCAATACAATCTACGAGACGCAATTGCACGGTGTACTGTTCAGGTGTATCCAAAGTCATGGAGTGCGATATATGTGGCGCTAGACAACGTTGGAATGTGGAATATAAGGACAGAATTTTGGGCAAGGCAATACTTAGGACAACAATTTTACTTAAGAGTTTACACTCCATCAACGTCATTAAGAGATGAGTACCCAATTCCAAAGAATGCTCTTCTTTGTGGAAGAGCCACCGGCAGAAGAACCAGGCCACTCTAATTTCCCCATTTATCATCATATCATTTACATAATCTTCTTCTTCCAGCTCGTAAAGAAAAAAAGAAAAAACCATTAATCATTATTGCTTCTCAAAGTTAAAATGTCCTTAGCTCGAGGTCatttatttatatatttttttctttaatttCTATTCATAGCATTTCATTGAATCGAATGTCCTTTCTTGATCTTCTTGCACTTGCAGAAGTCATTTTTTTTCTCTCTGTTTAGGTCATAAATATTCTTATGTTTGAGATAATTAAAGGCTCAATATATTAACA

Coding sequence (CDS)

ATGCCCTCCTCTCTCCTCTTCCTCTTCATTTCTTTCTTCTTCGCCATTGTCCGAGCTGAGGATCCTTACCGCTTCTTCGACTGGAATGTAACTTACGGCACCATTTACCCCCTCGGCCTCCCCCAGCAGGGCATTTTGATTAATGGCCAATTTCCTGGCCCCGATATTCACTCTGTTACCAACGACAATATCATTATCAATGTTTTCAATAGCTTGGATGAGCCTTTTCTCCTCTCCTGGAATGGGATTCAACAGAGAAGGAACTCGTATGAAGATGGTGTGTACGGAACAACTTGCCCCATTCCTCCAGGGAAGAACTTCACTTACATTCTTCAAATGAAAGATCAGATCGGAAGTTTCTATTACTTCCCTTCTCTTGGCTTCCATAAGGCCGCCGGTGGGTTTGGTGGAATCAGAATTCTTAGCCGTCCTCGGATTCCTGTTCCGTTCCCGGATCCTGCCGGGGATTATACAGTCCTTATCGGTGACTGGTACCAAGCTAATCATACGACGTTGAGGGCCCAATTAGACAATGGCTCAATGCTTCCTCTCCCCGATGGAATCCTCATCAATGGTCGTGGACCCAATAGAACTGCCTCAATCAACGTGGAACAAGGAAAAACTTACAGGCTGAGGATTTCAAACGTGGGGCTTCAAAGTTCTCTGAACTTCCGAATTCAAGGCCACAGAATGAAGGTGGTGGAAGTTGAAGGAACCCATACCCTCCAAACGGAATTCTCTTCCCTTGACATTCACGTAGGCCAATCTTATTCCGTCCTTGTCACTGCGGATCAGCCCGCTCAGGACTTTTACATTGTGGTTTCTTCTCGCTTTACTACTCCCATCCTCTCCACCACCGCTATTCTCCGTTATGCCAACTCCGCCGGCTCCGTCCAAGGCCCGCCTCCTGGTGGCCCAACTATCCAAATCGATTGGTCCCTTAATCAAGCTCGAGCCATCAGAACCAATCTTACAGCCAGTGGGCCACGGCCGAACCCACAAGGGTCGTATCACTATGGTATGATAACCACGACCAGAACCATTGTAGTGGCGAACTCAGCCGGAATTGTGGACGGAAAGCAAAGATACGCCGTGAACAGCGTGTCATTTATTCCGGCAGACACTCCGCTGAAGGTGGCAGACTTCTTCAAAATAGGAGGAGTTTTCCGGGTGGGAAGCATTTCCGACTGGCCAAACGGTGGAGGAATCTATCAAGACACCTCTGTTATGGGCGCCGATTACAGAGCTTTTGTCGAAATCGTTTTTCAGAACAACGAAGACCTCATTCAGAGCTGGCATCTTGATGGCTACTCCTTCTTCGTTGTTGGTATGGATGGAGGGCAATGGACATCGAATAGCAGAAACCAATACAATCTACGAGACGCAATTGCACGGTGTACTGTTCAGGTGTATCCAAAGTCATGGAGTGCGATATATGTGGCGCTAGACAACGTTGGAATGTGGAATATAAGGACAGAATTTTGGGCAAGGCAATACTTAGGACAACAATTTTACTTAAGAGTTTACACTCCATCAACGTCATTAAGAGATGAGTACCCAATTCCAAAGAATGCTCTTCTTTGTGGAAGAGCCACCGGCAGAAGAACCAGGCCACTCTAA

Protein sequence

MPSSLLFLFISFFFAIVRAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDNIIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLPLPDGILINGRGPNRTASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVEGTHTLQTEFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILSTTAILRYANSAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGMITTTRTIVVANSAGIVDGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSAIYVALDNVGMWNIRTEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL*
BLAST of Cucsa.394150 vs. Swiss-Prot
Match: ASOL_BRANA (L-ascorbate oxidase homolog OS=Brassica napus GN=Bp10 PE=2 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 8.9e-170
Identity = 288/522 (55.17%), Postives = 373/522 (71.46%), Query Frame = 1

Query: 16  IVRAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDNIIINVFNSLDEP 75
           +V AEDPY    WNVTYGT  PLG+PQQ ILINGQFPGP+I+S +N+N+IINVFN+LDEP
Sbjct: 20  VVHAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINSTSNNNVIINVFNNLDEP 79

Query: 76  FLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYFPSLGFHKAAGGF 135
           FLL+WNGIQ R+N ++DG  GT CPI PG N+TY  Q KDQIGS++Y+P+ G H+AAGG+
Sbjct: 80  FLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIGSYFYYPTTGMHRAAGGY 139

Query: 136 GGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLPLPDGILINGRGP 195
           GG+R+ SR  IPVP+ DP  DYTVLIGDWY  +HT L+  LD G  +  PDGI+ING+  
Sbjct: 140 GGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDGGRTIGRPDGIVINGKSG 199

Query: 196 NRTAS----INVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVEGTHTLQTEFSSLDI 255
               S      ++ GKTYR+RI NVG+++S+NFRIQ H+MK+VE+EG+H LQ ++ SLD+
Sbjct: 200 KGDGSDAPLFTLKPGKTYRVRICNVGVKTSINFRIQNHKMKLVEMEGSHVLQNDYDSLDV 259

Query: 256 HVGQSYSVLVTADQPAQDFYIVVSSRFTTPILSTTAILRYANSAGSVQGPPPGGPTIQID 315
           HVGQ +  +VTA+Q  +D+Y+V SSRF   +++TT +LRY    G      P GP +   
Sbjct: 260 HVGQCFGTIVTANQEPKDYYMVASSRFLKTVITTTGLLRYEGGKGPASSQLPAGP-VGWA 319

Query: 316 WSLNQARAIRTNLTASGPRPNPQGSYHYGMITTTRTIVVANSAGIVDGKQRYAVNSVSFI 375
           WSLNQ R+ R NLTAS  RPNPQGSYHYG I  TRTI + N+ G VDGK R+A+N VS  
Sbjct: 320 WSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQGKVDGKLRFALNGVSHT 379

Query: 376 PADTPLKVADFFKIGG-VFRVGSISDWPNGGGIYQ---DTSVMGADYRAFVEIVFQNNED 435
             +TPLK+A++F I   VF+  +I+D P    I     + +V+   +R FVE+VF+N+E 
Sbjct: 380 EPETPLKLAEYFGISDKVFKYDTITDDPTPEQIKNIKIEPNVLNITHRTFVEVVFENHEK 439

Query: 436 LIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSAIYVALDNVGM 495
            +QSWHLDGYSFF V ++ G WT   R  YNL DA++R TVQVYPK W+AI +  DN GM
Sbjct: 440 SVQSWHLDGYSFFSVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKCWAAILLTFDNCGM 499

Query: 496 WNIRTEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCG 530
           WN+R+E   R+YLGQQ Y  V +P  SLRDEY +P+ +L CG
Sbjct: 500 WNVRSENTERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCG 540

BLAST of Cucsa.394150 vs. Swiss-Prot
Match: ASOL_TOBAC (L-ascorbate oxidase homolog OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 3.9e-165
Identity = 283/527 (53.70%), Postives = 364/527 (69.07%), Query Frame = 1

Query: 17  VRAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDNIIINVFNSLDEPF 76
           V AEDPY +F+WNVTYGTI PLG+PQQGILINGQFPGP I+  +N+NI++NVFN+LDEPF
Sbjct: 19  VIAEDPYLYFNWNVTYGTIAPLGVPQQGILINGQFPGPRINCTSNNNIVVNVFNNLDEPF 78

Query: 77  LLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYFPSLGFHKAAGGFG 136
           L +WNG+Q R+NS++DG  GT CPI PG+NFTY  Q+KDQIGS+ YFP+   H+AAGG+G
Sbjct: 79  LFTWNGVQHRKNSWQDGTPGTMCPIMPGQNFTYRFQVKDQIGSYSYFPTTALHRAAGGYG 138

Query: 137 GIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLPLPDGILINGR--- 196
            + + SR  IPVPF +PA +Y V +GDWY   H TL+  LD G  +  PDGI+ING+   
Sbjct: 139 ALNVHSRALIPVPFDNPADEYNVFVGDWYNKGHKTLKKILDGGRTIGRPDGIIINGKSAK 198

Query: 197 -GPNRTASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVEGTHTLQTEFSSLDIH 256
            G  +     +E GKTYR R  N+G++SS+N R QGH MK+VE+EG+HT+Q  + SLD+H
Sbjct: 199 VGEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQGHPMKLVELEGSHTVQNIYDSLDLH 258

Query: 257 VGQSYSVLVTADQPAQDFYIVVSSRFTTPILSTTAILRYANSAGSVQ---GPPPGGPTIQ 316
           VGQ  SVLVTADQ  +D+Y+VVSSRF    LS+ AI+RYAN  G        PP   T  
Sbjct: 259 VGQCLSVLVTADQEPKDYYLVVSSRFLKQALSSVAIIRYANGKGPASPELPTPPPENTEG 318

Query: 317 IDWSLNQARAIRTNLTASGPRPNPQGSYHYGMITTTRTIVVANSAGIVDGKQRYAVNSVS 376
           I WS+NQ R+ R NLTAS  RPNPQGSYHYG I  TRTI + NS   V GK RY +N +S
Sbjct: 319 IAWSMNQFRSFRWNLTASAARPNPQGSYHYGQINITRTIKIFNSMSQVGGKLRYGLNGIS 378

Query: 377 FIPADTPLKVADFF-KIGGVFRVGSISDW--PNGGGIYQDTSVMGADYRAFVEIVFQNNE 436
               +TPLK+ ++F      F+   ++D    +   +   T+V  A YR FVEI+F+N+E
Sbjct: 379 HTNGETPLKLVEYFGATNKAFKYDLMADEAPADPSKLTIATNVKNATYRNFVEIIFENHE 438

Query: 437 DLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSAIYVALDNVG 496
             I+++HLDGYSFF V ++ G+W+   R  YNL D ++R  +QVYP SW+AI +  DN G
Sbjct: 439 KTIRTYHLDGYSFFAVAVEPGRWSPEKRKNYNLVDGLSRNNIQVYPNSWAAIMLTFDNAG 498

Query: 497 MWNIRTEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATG 534
           MWN+R+E W + YLG+Q Y  V +PS SLRDEY IP N  LCG   G
Sbjct: 499 MWNLRSEMWEKTYLGEQLYFSVLSPSRSLRDEYNIPDNHPLCGIVKG 545

BLAST of Cucsa.394150 vs. Swiss-Prot
Match: SKU5_ARATH (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana GN=SKU5 PE=1 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 1.7e-152
Identity = 280/544 (51.47%), Postives = 362/544 (66.54%), Query Frame = 1

Query: 5   LLFLFISFFFAIVRAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDNI 64
           + F+ ISF FA     DPY F+++ V+Y T  PLG+PQQ I ING+FPGP I+  TN+N+
Sbjct: 10  VFFVNISFCFAA----DPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTNENL 69

Query: 65  IINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYFP 124
           ++NV N LDE  LL WNGIQQRR S++DGV GT CPIPP  N+TY  Q+KDQIGSF+YFP
Sbjct: 70  VVNVRNKLDEGLLLHWNGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFFYFP 129

Query: 125 SLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLPL 184
           SL F +A+GGFG   +  R  IPVPF  P GD TV IGDWY  NHT LR  LD+G  L +
Sbjct: 130 SLHFQRASGGFGSFVVNPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDGKDLGM 189

Query: 185 PDGILINGRGPNR-----------TASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKV 244
           PDG+LING+GP R             +I V  GKTYRLR+SNVG+ +SLNFRIQGH + +
Sbjct: 190 PDGVLINGKGPYRYNDTLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHNLVL 249

Query: 245 VEVEGTHTLQTEFSSLDIHVGQSYSVLVTADQPA-QDFYIVVSSRFTTPIL----STTAI 304
            E EG++T+Q  ++SLDIHVGQSYS LVT DQ A  D+YIV S+R     +    +   I
Sbjct: 250 AESEGSYTVQQNYTSLDIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRVTGVGI 309

Query: 305 LRYANSAGSVQGPPPGGPTIQID--WSLNQARAIRTNLTASGPRPNPQGSYHYGMITTTR 364
           L+Y NS G  +G  P GP  + D  +S+NQAR+IR N++ASG RPNPQGS+ YG I  T 
Sbjct: 310 LKYTNSKGKAKGQLPPGPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGSINVTD 369

Query: 365 TIVVANSAGI-VDGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQ 424
             V+ N   + + GK+R  +N +SF    TP+++AD  K+  V+++      P  G    
Sbjct: 370 VYVLRNMPPVTISGKRRTTLNGISFKNPSTPIRLADKLKVKDVYKL-DFPKRPLTGPAKV 429

Query: 425 DTSVMGADYRAFVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIAR 484
            TS++   YR F+E+V QNN+  +QS+H+ GY+FFVVGMD G+WT NSR  YN  D IAR
Sbjct: 430 ATSIINGTYRGFMEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGTYNKWDGIAR 489

Query: 485 CTVQVYPKSWSAIYVALDNVGMWNIRTEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNA 530
            T+QVYP +WSAI ++LDN G WN+RTE     YLGQ+ Y+RV  P  + + E+  P N 
Sbjct: 490 STIQVYPGAWSAILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNKTEFGHPDNV 548

BLAST of Cucsa.394150 vs. Swiss-Prot
Match: SKS1_ARATH (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana GN=SKS1 PE=1 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 2.5e-148
Identity = 269/547 (49.18%), Postives = 362/547 (66.18%), Query Frame = 1

Query: 4   SLLFLFISFFF---AIVRAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVT 63
           SLL  F+  F    A+  A DP+  +D+ V+Y T  PLG+PQQ I +NGQFPGP +++ T
Sbjct: 6   SLLASFLLCFALLSAVSFAADPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPLLNATT 65

Query: 64  NDNIIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSF 123
           N N+++NVFN LDEP LL+W GIQ RRNS++DGV GT CPIPP  NFTY  Q+KDQIGSF
Sbjct: 66  NYNVVVNVFNHLDEPLLLTWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQVKDQIGSF 125

Query: 124 YYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGS 183
           +Y PSL F +A+GGFG I I +R  IP+PFP P G+   +IGDWY  +H  LR  LD+G 
Sbjct: 126 FYSPSLNFQRASGGFGPIVINNRDIIPIPFPQPDGELIFIIGDWYTQDHKALRRALDSGK 185

Query: 184 MLPLPDGILINGRGPNRTAS----------INVEQGKTYRLRISNVGLQSSLNFRIQGHR 243
            L +PDG+LING+GP +  S           +VE GKTYR+R+ NVG+ +SLNFRIQ H 
Sbjct: 186 ELGMPDGVLINGKGPYKYNSSVPDGIDYLTFHVEPGKTYRIRVHNVGISTSLNFRIQNHS 245

Query: 244 MKVVEVEGTHTLQTEFSSLDIHVGQSYSVLVTADQPA-QDFYIVVSSRFTTPI----LST 303
           + +VE EG +T Q  F+  D+HVGQSYS LVT DQ A  D+YIV S+RF        ++ 
Sbjct: 246 LLLVETEGHYTSQANFTDFDVHVGQSYSFLVTMDQDATSDYYIVASARFVNETVWQRVTG 305

Query: 304 TAILRYANSAGSVQGPPPGGPT-IQIDWS-LNQARAIRTNLTASGPRPNPQGSYHYGMIT 363
            AIL Y+NS G V GP P   T +   WS ++Q + IR N +ASG RPNPQGS+HYG I 
Sbjct: 306 VAILHYSNSKGPVSGPLPVPKTDVSSPWSAMSQPKTIRQNTSASGARPNPQGSFHYGQIN 365

Query: 364 TTRTIVVAN-SAGIVDGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGG 423
            T T ++ +    I++G  R  +N +SF+   TP+++AD  K+ G +++    D P    
Sbjct: 366 ITNTYILRSLPPTIINGALRATLNGISFVNPSTPVRLADRNKVKGAYKL-DFPDRPFNRP 425

Query: 424 IYQDTSVMGADYRAFVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDA 483
           +  D S++ A Y+ F+++VFQNN+  IQS+H+DGYSFFVVGMD G W+ + +  YN  DA
Sbjct: 426 LRLDRSMINATYKGFIQVVFQNNDTKIQSFHVDGYSFFVVGMDFGIWSEDKKGSYNNWDA 485

Query: 484 IARCTVQVYPKSWSAIYVALDNVGMWNIRTEFWARQYLGQQFYLRVYTPSTSLRDEYPIP 530
           I+R T++VYP  W+A+ ++LDNVG+WNIR E   R YLG++ Y+R+  P    + E   P
Sbjct: 486 ISRSTIEVYPGGWTAVLISLDNVGVWNIRVENLDRWYLGEETYMRITNPEEDGKTEMDPP 545

BLAST of Cucsa.394150 vs. Swiss-Prot
Match: SKS2_ARATH (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana GN=SKS2 PE=1 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 9.0e-146
Identity = 260/552 (47.10%), Postives = 365/552 (66.12%), Query Frame = 1

Query: 1   MPSSLLFLFISFFFAIVR----AEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDI 60
           M ++  F    F FA++     A DPY  +D+ ++Y T  PLG+PQQ I +NG+FPGP I
Sbjct: 1   MAATDFFFAFVFSFALIFGFSFAGDPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVI 60

Query: 61  HSVTNDNIIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQ 120
           ++ TN N+ +NV N LDEP LL+W G+Q RRNS++DGV GT CPIPP  NFTY  Q+KDQ
Sbjct: 61  NATTNYNVHVNVLNHLDEPLLLTWPGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKDQ 120

Query: 121 IGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQL 180
           IGS++Y PSL F +A+GGFG + I +R  +P+PF +P G+   +IGDWY  NHT LR  L
Sbjct: 121 IGSYFYSPSLNFQRASGGFGALIINNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRIL 180

Query: 181 DNGSMLPLPDGILINGRGPNRTAS----------INVEQGKTYRLRISNVGLQSSLNFRI 240
           D+G  L +PDG+LING+GP +  S          +NV+ GKTYR+R+ NVG+ +SLNFRI
Sbjct: 181 DSGKELGMPDGVLINGKGPFKYNSSVPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFRI 240

Query: 241 QGHRMKVVEVEGTHTLQTEFSSLDIHVGQSYSVLVTADQPA-QDFYIVVSSRFTTPI--- 300
           Q H++ ++E EG +T Q  F+  D+HVGQSYS LVT DQ A  D+YIV S+RF       
Sbjct: 241 QNHKLLLIETEGRYTSQMNFTDFDVHVGQSYSFLVTMDQNATSDYYIVASARFVNETVWQ 300

Query: 301 -LSTTAILRYANSAGSVQGPPPGGPT-IQIDWS-LNQARAIRTNLTASGPRPNPQGSYHY 360
            ++   IL Y+NS G   GP P   T +   WS +NQ RAI+ N +ASG RPNPQGS+HY
Sbjct: 301 RVTGVGILHYSNSKGPASGPLPVSATDVNHPWSAMNQPRAIKQNTSASGARPNPQGSFHY 360

Query: 361 GMITTTRTIVVANSAGI-VDGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWP 420
           G I  TRT ++ +     ++GK R  +N +SF+   TP+++AD  K+ G + +    D P
Sbjct: 361 GQINITRTYILRSLPPTKINGKLRATLNGISFVNPSTPMRLADDHKVKGDYML-DFPDRP 420

Query: 421 NGGGIYQ-DTSVMGADYRAFVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQY 480
               + +  +S++ A Y+ F++++FQNN+  IQS+H+DGY+F+VV MD G W+ +  + Y
Sbjct: 421 LDEKLPRLSSSIINATYKGFIQVIFQNNDTKIQSFHIDGYAFYVVAMDFGIWSEDRNSSY 480

Query: 481 NLRDAIARCTVQVYPKSWSAIYVALDNVGMWNIRTEFWARQYLGQQFYLRVYTPSTSLRD 530
           N  DA+AR TV+VYP +W+A+ ++LDNVG+WNIR E   R YLGQ+ Y+R+  P  +   
Sbjct: 481 NNWDAVARSTVEVYPGAWTAVLISLDNVGVWNIRVENLDRWYLGQETYMRIINPEENGST 540

BLAST of Cucsa.394150 vs. TrEMBL
Match: A0A0A0LN97_CUCSA (Multicopper oxidase OS=Cucumis sativus GN=Csa_2G348200 PE=4 SV=1)

HSP 1 Score: 1109.4 bits (2868), Expect = 0.0e+00
Identity = 539/539 (100.00%), Postives = 539/539 (100.00%), Query Frame = 1

Query: 1   MPSSLLFLFISFFFAIVRAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVT 60
           MPSSLLFLFISFFFAIVRAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVT
Sbjct: 1   MPSSLLFLFISFFFAIVRAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVT 60

Query: 61  NDNIIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSF 120
           NDNIIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSF
Sbjct: 61  NDNIIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSF 120

Query: 121 YYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGS 180
           YYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGS
Sbjct: 121 YYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGS 180

Query: 181 MLPLPDGILINGRGPNRTASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVEGTH 240
           MLPLPDGILINGRGPNRTASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVEGTH
Sbjct: 181 MLPLPDGILINGRGPNRTASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVEGTH 240

Query: 241 TLQTEFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILSTTAILRYANSAGSVQG 300
           TLQTEFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILSTTAILRYANSAGSVQG
Sbjct: 241 TLQTEFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILSTTAILRYANSAGSVQG 300

Query: 301 PPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGMITTTRTIVVANSAGIVDGK 360
           PPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGMITTTRTIVVANSAGIVDGK
Sbjct: 301 PPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGMITTTRTIVVANSAGIVDGK 360

Query: 361 QRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFVEI 420
           QRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFVEI
Sbjct: 361 QRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFVEI 420

Query: 421 VFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSAIYV 480
           VFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSAIYV
Sbjct: 421 VFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSAIYV 480

Query: 481 ALDNVGMWNIRTEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL 540
           ALDNVGMWNIRTEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL
Sbjct: 481 ALDNVGMWNIRTEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL 539

BLAST of Cucsa.394150 vs. TrEMBL
Match: A0A061FD20_THECC (SKU5 similar 5 isoform 1 OS=Theobroma cacao GN=TCM_034334 PE=4 SV=1)

HSP 1 Score: 956.1 bits (2470), Expect = 1.9e-275
Identity = 460/542 (84.87%), Postives = 498/542 (91.88%), Query Frame = 1

Query: 1   MPSSL---LFLFISFFFAIVRAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIH 60
           MP +L   LF   +  FAI  A+DPYRFF+WNVTYG IYPLG+ Q GILINGQFPGPDIH
Sbjct: 1   MPLNLAVALFCATASLFAIAGADDPYRFFNWNVTYGDIYPLGVRQTGILINGQFPGPDIH 60

Query: 61  SVTNDNIIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQI 120
           SVTNDN+IINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQ+KDQI
Sbjct: 61  SVTNDNLIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQVKDQI 120

Query: 121 GSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLD 180
           GSFYYFPSL FHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWY++NHT LRA LD
Sbjct: 121 GSFYYFPSLAFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYKSNHTDLRAHLD 180

Query: 181 NGSMLPLPDGILINGRGPNRTASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVE 240
            G  LP PDGILINGRGP   AS NVEQGKTYRLRISNVGLQ+SLNFRIQ H++K+VEVE
Sbjct: 181 LGKKLPFPDGILINGRGPGG-ASFNVEQGKTYRLRISNVGLQNSLNFRIQNHKLKLVEVE 240

Query: 241 GTHTLQTEFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILSTTAILRYANSAGS 300
           GTHTLQT +SS+DIHVGQS SVL TADQPAQD+YIVVS+RFT P+L+TTA LRY+NSAG 
Sbjct: 241 GTHTLQTTYSSIDIHVGQSSSVLFTADQPAQDYYIVVSTRFTNPVLTTTATLRYSNSAGP 300

Query: 301 VQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGMITTTRTIVVANSAGIV 360
           V GPPPGGPTIQIDWSLNQAR+IRTNLTASGPRPNPQGSYHYG+I TTRTI +ANSAG V
Sbjct: 301 VSGPPPGGPTIQIDWSLNQARSIRTNLTASGPRPNPQGSYHYGLINTTRTIRLANSAGQV 360

Query: 361 DGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAF 420
           +GKQRYAVNSVSF+PADTPLK+AD+FKIGGVFRVGSISD P GGGIY DTSVM ADYRAF
Sbjct: 361 NGKQRYAVNSVSFVPADTPLKLADYFKIGGVFRVGSISDNPYGGGIYLDTSVMNADYRAF 420

Query: 421 VEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSA 480
           +EIVFQN+ED++QSWHLDGYSFFVVGMDGGQWT+ SRNQYNLRDAI+RCT QVYPKSW+A
Sbjct: 421 IEIVFQNDEDIVQSWHLDGYSFFVVGMDGGQWTAASRNQYNLRDAISRCTTQVYPKSWTA 480

Query: 481 IYVALDNVGMWNIRTEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTR 540
           IYVALDNVGMWN+R+EFWARQYLGQQFYLRVYT S+SLRDEYPIPKNALLCGRA+GR TR
Sbjct: 481 IYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTDSSSLRDEYPIPKNALLCGRASGRSTR 540

BLAST of Cucsa.394150 vs. TrEMBL
Match: M5XC21_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003860mg PE=4 SV=1)

HSP 1 Score: 954.5 bits (2466), Expect = 5.5e-275
Identity = 449/532 (84.40%), Postives = 490/532 (92.11%), Query Frame = 1

Query: 8   LFISFFFAIVRAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDNIIIN 67
           LF++  F+IV AEDPYRFF+WNVTYG IYPLG+ Q+GILINGQFPGPDIHSVTNDN+IIN
Sbjct: 14  LFLTSLFSIVAAEDPYRFFEWNVTYGDIYPLGVRQKGILINGQFPGPDIHSVTNDNLIIN 73

Query: 68  VFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYFPSLG 127
           VFNSLDEPFL+SWNGIQQRRNS+EDGVYGTTCPIPPG+NFTYILQ+KDQIGSFYYFPSL 
Sbjct: 74  VFNSLDEPFLISWNGIQQRRNSFEDGVYGTTCPIPPGRNFTYILQVKDQIGSFYYFPSLA 133

Query: 128 FHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLPLPDG 187
           FHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWY++NHT+L+A LD G  LP PDG
Sbjct: 134 FHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYKSNHTSLKAHLDAGKKLPFPDG 193

Query: 188 ILINGRGPNRTASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVEGTHTLQTEFS 247
           ILINGRGP    S N EQGKTYRLRISNVGLQ SLNFRIQ H++K+VEVEGTHTLQT +S
Sbjct: 194 ILINGRGPGGF-SFNFEQGKTYRLRISNVGLQHSLNFRIQNHKLKLVEVEGTHTLQTTYS 253

Query: 248 SLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILSTTAILRYANSAGSVQGPPPGGPT 307
           SLD+HVGQSYSVLVTADQP QD+YIV SSRFTTPIL+TT  L Y NSAG V GPPPGGPT
Sbjct: 254 SLDVHVGQSYSVLVTADQPGQDYYIVASSRFTTPILTTTGTLHYTNSAGRVSGPPPGGPT 313

Query: 308 IQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGMITTTRTIVVANSAGIVDGKQRYAVNS 367
           IQ+DWSLNQAR+IRTNLTASGPRPNPQGSYHYG+I TT+T V+ NSAG V+GKQRY VNS
Sbjct: 314 IQVDWSLNQARSIRTNLTASGPRPNPQGSYHYGLINTTKTYVLENSAGQVNGKQRYGVNS 373

Query: 368 VSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFVEIVFQNNED 427
           VSF+PADTPLK+AD+FKIGGVFRVGSISD P GGG+Y DTSV+GADYR FVE VFQNNED
Sbjct: 374 VSFVPADTPLKLADYFKIGGVFRVGSISDRPTGGGLYLDTSVLGADYRTFVEFVFQNNED 433

Query: 428 LIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSAIYVALDNVGM 487
           +IQSWHLDGYSFFVVG+DGGQWT  SR +YNLRDA++RCTVQVYPKSW+AIYVALDNVGM
Sbjct: 434 IIQSWHLDGYSFFVVGLDGGQWTPASRKEYNLRDAVSRCTVQVYPKSWTAIYVALDNVGM 493

Query: 488 WNIRTEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL 540
           WN+RTEFWARQYLGQQ YLRVYTPSTS+RDEYPIPKNALLCGRATGRRTRPL
Sbjct: 494 WNLRTEFWARQYLGQQLYLRVYTPSTSIRDEYPIPKNALLCGRATGRRTRPL 544

BLAST of Cucsa.394150 vs. TrEMBL
Match: E5GCD6_CUCME (Multicopper oxidase OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 952.6 bits (2461), Expect = 2.1e-274
Identity = 450/527 (85.39%), Postives = 493/527 (93.55%), Query Frame = 1

Query: 13  FFAIVRAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDNIIINVFNSL 72
           FF+ + AE+PYRFF WNV+Y  IYPLG+ QQGILINGQFPGPDIH VTNDN+IINVFNSL
Sbjct: 17  FFSTLSAENPYRFFTWNVSYANIYPLGVRQQGILINGQFPGPDIHCVTNDNLIINVFNSL 76

Query: 73  DEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYFPSLGFHKAA 132
           DEPFL+SWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQ+KDQIGSFYYFPSL FHKAA
Sbjct: 77  DEPFLISWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAA 136

Query: 133 GGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLPLPDGILING 192
           GGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWY++NHTTL+A LD G  LP PDGILING
Sbjct: 137 GGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYKSNHTTLKAHLDRGKKLPFPDGILING 196

Query: 193 RGPNRTASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVEGTHTLQTEFSSLDIH 252
           R  +   S +VEQGKTYRLRISNVGLQ SLNFRIQGH+MK+VEVEGTHTLQT +SSLD+H
Sbjct: 197 RAND--TSFSVEQGKTYRLRISNVGLQHSLNFRIQGHKMKLVEVEGTHTLQTTYSSLDVH 256

Query: 253 VGQSYSVLVTADQPAQDFYIVVSSRFTTPILSTTAILRYANSAGSVQGPPPGGPTIQIDW 312
           VGQSYSVLVTADQPAQD+YIVVS+RFT+ +L+TT ILRY+NSAG V+GPPPGGPTIQIDW
Sbjct: 257 VGQSYSVLVTADQPAQDYYIVVSTRFTSRVLATTGILRYSNSAGPVKGPPPGGPTIQIDW 316

Query: 313 SLNQARAIRTNLTASGPRPNPQGSYHYGMITTTRTIVVANSAGIVDGKQRYAVNSVSFIP 372
           SLNQAR+IRTNLTASGPRPNPQGSYHYG+I TT+TI++ANSAG V+ KQRYAVNSVSF+P
Sbjct: 317 SLNQARSIRTNLTASGPRPNPQGSYHYGLINTTKTIILANSAGQVNRKQRYAVNSVSFVP 376

Query: 373 ADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFVEIVFQNNEDLIQSW 432
           ADTPLK+ADFFKIGGVFRVGSISD P GGGIY DTSVMGADYRAFVEIVFQNNE+++QSW
Sbjct: 377 ADTPLKLADFFKIGGVFRVGSISDRPTGGGIYLDTSVMGADYRAFVEIVFQNNENIVQSW 436

Query: 433 HLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSAIYVALDNVGMWNIRT 492
           H+DGYSFFVVGMDGGQWT +SRNQYNLRDAIARCT QVYP SW+AIYVALDNVG+WN+R+
Sbjct: 437 HIDGYSFFVVGMDGGQWTQSSRNQYNLRDAIARCTTQVYPYSWTAIYVALDNVGLWNVRS 496

Query: 493 EFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL 540
           EFWARQYLGQQFYLRVYTPSTSLRDE+PIPKNALLCGRA+GR TRPL
Sbjct: 497 EFWARQYLGQQFYLRVYTPSTSLRDEFPIPKNALLCGRASGRHTRPL 541

BLAST of Cucsa.394150 vs. TrEMBL
Match: A0A0D2QIY7_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_009G189900 PE=4 SV=1)

HSP 1 Score: 945.3 bits (2442), Expect = 3.3e-272
Identity = 445/526 (84.60%), Postives = 491/526 (93.35%), Query Frame = 1

Query: 14  FAIVRAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDNIIINVFNSLD 73
           F I  AEDPYRFF+WNVTYG I+PLG+ Q GILINGQFPGPDIHSVTNDN+IINVFNSL+
Sbjct: 17  FVIAGAEDPYRFFNWNVTYGDIFPLGVRQTGILINGQFPGPDIHSVTNDNLIINVFNSLN 76

Query: 74  EPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYFPSLGFHKAAG 133
           EPFLLSWNG+Q RRNSYEDGVYGTTCPIPPGKNFTYILQ+KDQIGSFYYFPSLGFHKAAG
Sbjct: 77  EPFLLSWNGVQNRRNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLGFHKAAG 136

Query: 134 GFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLPLPDGILINGR 193
           GFGGIRILSRPRIPVPFPDPAGDYTVLIGDWY+ANHT LRAQLD+G  LP PDGILINGR
Sbjct: 137 GFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYKANHTDLRAQLDSGRKLPFPDGILINGR 196

Query: 194 GPNRTASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVEGTHTLQTEFSSLDIHV 253
           GP   AS NVEQGKTYRLRISNVGLQ+SLNFRIQ H++K+VEVEGTHTLQT +SS+D+H+
Sbjct: 197 GPGG-ASFNVEQGKTYRLRISNVGLQNSLNFRIQNHKLKLVEVEGTHTLQTTYSSIDLHL 256

Query: 254 GQSYSVLVTADQPAQDFYIVVSSRFTTPILSTTAILRYANSAGSVQGPPPGGPTIQIDWS 313
           GQS SVL TADQPAQD+YIVVSSRFT P+L++TA LRY+NSAG V GPPPGGPTIQ+DWS
Sbjct: 257 GQSMSVLFTADQPAQDYYIVVSSRFTNPVLTSTATLRYSNSAGPVSGPPPGGPTIQVDWS 316

Query: 314 LNQARAIRTNLTASGPRPNPQGSYHYGMITTTRTIVVANSAGIVDGKQRYAVNSVSFIPA 373
           LNQAR+IRTNLTASGPRPNPQGSYHYG+I TTRTI +ANSAG V+GKQRYAVNSVSF+PA
Sbjct: 317 LNQARSIRTNLTASGPRPNPQGSYHYGLINTTRTIRLANSAGQVNGKQRYAVNSVSFVPA 376

Query: 374 DTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFVEIVFQNNEDLIQSWH 433
           DTPLK+ADFFKI GV+R+GSISD P GGGIY DTSVM +DYR+F+EIVFQN+ED++QSWH
Sbjct: 377 DTPLKLADFFKIDGVYRIGSISDNPTGGGIYLDTSVMNSDYRSFIEIVFQNDEDIVQSWH 436

Query: 434 LDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSAIYVALDNVGMWNIRTE 493
           LDGYSFFVVGMDGGQWT+ SRN YNLRDA++RCT QVYPKSW+AIYVALDNVGMWN+R+E
Sbjct: 437 LDGYSFFVVGMDGGQWTAASRNGYNLRDAVSRCTTQVYPKSWTAIYVALDNVGMWNLRSE 496

Query: 494 FWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL 540
           +WARQYLGQQFYLRV+T STSLRDEYPIPKNALLCGRA+GRRTRPL
Sbjct: 497 YWARQYLGQQFYLRVFTTSTSLRDEYPIPKNALLCGRASGRRTRPL 541

BLAST of Cucsa.394150 vs. TAIR10
Match: AT1G76160.1 (AT1G76160.1 SKU5 similar 5)

HSP 1 Score: 926.4 bits (2393), Expect = 8.1e-270
Identity = 442/537 (82.31%), Postives = 487/537 (90.69%), Query Frame = 1

Query: 3   SSLLFLFISFFFAIVRAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTND 62
           ++ LF+ +S  FA+  AEDPYRFF+WN+TYG IYPLG+ QQGILING FPGPDIHSVTND
Sbjct: 8   AAALFIGLSLLFAVT-AEDPYRFFEWNITYGDIYPLGVRQQGILINGAFPGPDIHSVTND 67

Query: 63  NIIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYY 122
           N+IINV+NSLDEPFLLSWNGIQQRRNS+ DGVYGTTCPIPPGKN+TYILQMKDQIGSFYY
Sbjct: 68  NLIINVYNSLDEPFLLSWNGIQQRRNSFVDGVYGTTCPIPPGKNYTYILQMKDQIGSFYY 127

Query: 123 FPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSML 182
           FPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGD TVLIGDWY+ANHT LRAQLDNG  L
Sbjct: 128 FPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDTTVLIGDWYKANHTDLRAQLDNGKKL 187

Query: 183 PLPDGILINGRGPNRTASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVEGTHTL 242
           PLPDGILINGR     A++NVEQGKTYR RISNVGLQ SLNFRIQ H+MKVVEVEGTHTL
Sbjct: 188 PLPDGILINGRSSG--ATLNVEQGKTYRFRISNVGLQDSLNFRIQDHKMKVVEVEGTHTL 247

Query: 243 QTEFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILSTTAILRYANSAGSVQGPP 302
           QT FSSLD+HVGQSYSVLVTADQ  +D+Y+VVSSRFT+ +L+TT I RY+NSAG V GP 
Sbjct: 248 QTTFSSLDVHVGQSYSVLVTADQTPRDYYVVVSSRFTSNVLTTTGIFRYSNSAGGVSGPI 307

Query: 303 PGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGMITTTRTIVVANSAGIVDGKQR 362
           PGGPTIQIDWSLNQARAIRTNL+ASGPRPNPQGSYHYGMI TTRTI +A+SAG VDGKQR
Sbjct: 308 PGGPTIQIDWSLNQARAIRTNLSASGPRPNPQGSYHYGMINTTRTIRLASSAGQVDGKQR 367

Query: 363 YAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFVEIVF 422
           YAVNSVSF PADTPLK+AD+FKI GV+R GSI   P GGGIY DTSVM  DYR FVEI+F
Sbjct: 368 YAVNSVSFKPADTPLKIADYFKIDGVYRSGSIQYQPTGGGIYLDTSVMQVDYRTFVEIIF 427

Query: 423 QNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSAIYVAL 482
           +N+ED++QSWHLDGYSF+VVGMDGGQW+ +SRN+YNLRDA+ARCTVQVYP SW+AI +AL
Sbjct: 428 ENSEDIVQSWHLDGYSFWVVGMDGGQWSPDSRNEYNLRDAVARCTVQVYPSSWTAILIAL 487

Query: 483 DNVGMWNIRTEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL 540
           DNVGMWN+R+EFWARQYLGQQ YLRVYTPSTSLRDEYPIPKNALLCGRA+GR TRPL
Sbjct: 488 DNVGMWNLRSEFWARQYLGQQLYLRVYTPSTSLRDEYPIPKNALLCGRASGRSTRPL 541

BLAST of Cucsa.394150 vs. TAIR10
Match: AT1G41830.1 (AT1G41830.1 SKU5-similar 6)

HSP 1 Score: 874.0 bits (2257), Expect = 4.7e-254
Identity = 413/534 (77.34%), Postives = 467/534 (87.45%), Query Frame = 1

Query: 6   LFLFISFFFAIVRAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDNII 65
           + LF   FFA V AE PYRFFDWNVTYG IYPLG+ QQGILINGQFPGPDIHSVTNDN+I
Sbjct: 11  ILLFCLSFFAAVTAESPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPDIHSVTNDNLI 70

Query: 66  INVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYFPS 125
           INV NSLDEPFL+SWNG+Q RRNSY DG+YGTTCPIPP  N+TYILQ+KDQIGSFYYFPS
Sbjct: 71  INVHNSLDEPFLISWNGVQNRRNSYVDGMYGTTCPIPPRSNYTYILQVKDQIGSFYYFPS 130

Query: 126 LGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLPLP 185
           L FHKAAGGFGGIRILSRP IPVPF DPAGDYTVLIGDWY+ NHT L+++LD G  LP P
Sbjct: 131 LAFHKAAGGFGGIRILSRPGIPVPFADPAGDYTVLIGDWYKFNHTDLKSRLDRGRKLPSP 190

Query: 186 DGILINGRGPNRTASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVEGTHTLQTE 245
           DGILINGR  +  A++NVEQGKTYRLRISNVGLQ SLNFRIQ HRMK+VEVEGTHTLQT 
Sbjct: 191 DGILINGR--SNGATLNVEQGKTYRLRISNVGLQDSLNFRIQNHRMKLVEVEGTHTLQTM 250

Query: 246 FSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILSTTAILRYANSAGSVQGPPPGG 305
           FSSLD+HVGQSYSVL+TADQ  +D+Y+VVSSRFT  I++TT +LRY+ S+    GP PGG
Sbjct: 251 FSSLDVHVGQSYSVLITADQSPRDYYVVVSSRFTDKIITTTGVLRYSGSSTPASGPIPGG 310

Query: 306 PTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGMITTTRTIVVANSAGIVDGKQRYAV 365
           PTIQ+DWSLNQARAIRTNLTASGPRPNPQGSYHYG+I   RTIV  +SAG ++GKQRY V
Sbjct: 311 PTIQVDWSLNQARAIRTNLTASGPRPNPQGSYHYGLIPLIRTIVFGSSAGQINGKQRYGV 370

Query: 366 NSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFVEIVFQNN 425
           NSVSF+PADTPLK+ADFFKI GV+++ SISD P  GG+Y DTSV+  DYR F+EIVF+N 
Sbjct: 371 NSVSFVPADTPLKLADFFKISGVYKINSISDKPTYGGLYLDTSVLQVDYRTFIEIVFENQ 430

Query: 426 EDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSAIYVALDNV 485
           ED++QS+HL+GYSF+VVGMDGGQW + SRN YNLRDA++R TVQVYPKSW+AIY+ALDNV
Sbjct: 431 EDIVQSYHLNGYSFWVVGMDGGQWKTGSRNGYNLRDAVSRSTVQVYPKSWTAIYIALDNV 490

Query: 486 GMWNIRTEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL 540
           GMWN+R+EFWARQYLGQQ YLRV+T STSLRDEYPIPKN+ LCGRA GR TRPL
Sbjct: 491 GMWNLRSEFWARQYLGQQLYLRVFTSSTSLRDEYPIPKNSRLCGRARGRHTRPL 542

BLAST of Cucsa.394150 vs. TAIR10
Match: AT1G21850.1 (AT1G21850.1 SKU5 similar 8)

HSP 1 Score: 831.2 bits (2146), Expect = 3.5e-241
Identity = 395/534 (73.97%), Postives = 456/534 (85.39%), Query Frame = 1

Query: 3   SSLLFLFISFFFAIVRAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTND 62
           ++ + L + F  + V AEDPY+FF+W+VTYG I PL + QQGILING+FPGPDI +VTND
Sbjct: 8   TTAMILGLFFLISFVAAEDPYKFFEWHVTYGNISPLKVAQQGILINGKFPGPDIAAVTND 67

Query: 63  NIIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYY 122
           N+IINVFN LDEPFL+SW+GI+  RNSY+DGVYGTTCPIPPGKN+TY LQ+KDQIGSFYY
Sbjct: 68  NLIINVFNHLDEPFLISWSGIRNWRNSYQDGVYGTTCPIPPGKNYTYALQVKDQIGSFYY 127

Query: 123 FPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSML 182
           FPSLGFHKAAGGFG IRI SRPRIPVPFP PAGDYTVLIGDWY+ NH  LRAQLDNG  L
Sbjct: 128 FPSLGFHKAAGGFGAIRISSRPRIPVPFPAPAGDYTVLIGDWYKTNHKDLRAQLDNGGKL 187

Query: 183 PLPDGILINGRGPNRTASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVEGTHTL 242
           P PDGILINGRG    A++N+E GKTYRLRISNVGLQ+SLNFRIQ H+MK+VEVEGTHT+
Sbjct: 188 PFPDGILINGRGSG--ATLNIEPGKTYRLRISNVGLQNSLNFRIQNHKMKLVEVEGTHTI 247

Query: 243 QTEFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILSTTAILRYANSAGSVQGPP 302
           QT FSSLD+HVGQSYSVL+TADQPA+D+YIVVSSRFT+ IL T  +L Y+NSAG V GP 
Sbjct: 248 QTPFSSLDVHVGQSYSVLITADQPAKDYYIVVSSRFTSKILITAGVLHYSNSAGPVSGPI 307

Query: 303 PGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGMITTTRTIVVANSAGIVDGKQR 362
           P  P IQ+ WS +QARAI+TNL ASGPRPNPQG+YHYG I  TRTI +A+SAG ++GKQR
Sbjct: 308 PEAP-IQLRWSFDQARAIKTNLAASGPRPNPQGTYHYGKIKVTRTIKLASSAGNINGKQR 367

Query: 363 YAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFVEIVF 422
           YAVNS SF P DTPLK+AD+FKI GV+  GSI D P  G IY  TSVM  DY+AFVEIVF
Sbjct: 368 YAVNSASFYPTDTPLKLADYFKIAGVYNPGSIPDQPTHGAIYPVTSVMQTDYKAFVEIVF 427

Query: 423 QNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSAIYVAL 482
           +N ED++Q+WHLDGYSFFVVGM+ G+W++ SR  YNL DA++RCTVQVYP+SW+AIYV+L
Sbjct: 428 ENWEDIVQTWHLDGYSFFVVGMELGKWSAASRKVYNLNDAVSRCTVQVYPRSWTAIYVSL 487

Query: 483 DNVGMWNIRTEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRT 537
           DNVGMWN+R+E W RQYLGQQFY+RVYTPSTSLRDEY IPKNALLCGRATG  T
Sbjct: 488 DNVGMWNLRSELWERQYLGQQFYMRVYTPSTSLRDEYLIPKNALLCGRATGHHT 538

BLAST of Cucsa.394150 vs. TAIR10
Match: AT1G21860.1 (AT1G21860.1 SKU5 similar 7)

HSP 1 Score: 811.2 bits (2094), Expect = 3.8e-235
Identity = 388/532 (72.93%), Postives = 453/532 (85.15%), Query Frame = 1

Query: 1   MPSSLLFLFISFFFAIVRAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVT 60
           M ++LLFL IS  FA    EDPYRFF+W+VTYG I PLG+ QQGILING+FPGPDI S+T
Sbjct: 11  MITTLLFL-ISLAFA----EDPYRFFEWHVTYGNISPLGVAQQGILINGKFPGPDIISIT 70

Query: 61  NDNIIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSF 120
           NDN+IINVFN LDEPFLLSWNGI+  +NS++DGVYGT CPIPPGKN+TY LQ+KDQIGSF
Sbjct: 71  NDNLIINVFNHLDEPFLLSWNGIRNWKNSFQDGVYGTMCPIPPGKNYTYALQVKDQIGSF 130

Query: 121 YYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGS 180
           YYFPSLGFHKAAGGFGGIRI SR  IPVPFP PA DYT+L+GDWY+ NH  L+AQLDNG 
Sbjct: 131 YYFPSLGFHKAAGGFGGIRISSRALIPVPFPTPADDYTLLVGDWYKTNHKDLKAQLDNGG 190

Query: 181 MLPLPDGILINGRGPNRTASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVEGTH 240
            LPLPDGILINGR     A++N+E GKTYRLRISNVGLQ+SLNFRIQ H MK+VEVEG +
Sbjct: 191 KLPLPDGILINGRSSG--ATLNIEPGKTYRLRISNVGLQNSLNFRIQNHTMKLVEVEGRY 250

Query: 241 TLQTEFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILSTTAILRYANSAGSVQG 300
           T+Q  FSSLD+HVGQSYSVL+TADQPA+D+Y+VVSSRFT+ IL+TT +L Y+NS   V G
Sbjct: 251 TIQNLFSSLDVHVGQSYSVLITADQPAKDYYVVVSSRFTSKILTTTGVLHYSNSVAPVSG 310

Query: 301 PPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGMITTTRTIVVANSAGIVDGK 360
           P P GP I++ WS NQARAIRTNLTASGPRPNPQGSY YG+I  TRTI +AN+ G ++GK
Sbjct: 311 PIPDGP-IKLSWSFNQARAIRTNLTASGPRPNPQGSYRYGVINITRTIRLANNLGHIEGK 370

Query: 361 QRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFVEI 420
           QRYAVNS SF PADTPLK+ D+FKI GV++ GSISD P  G I+  TSVM AD+RAFVE+
Sbjct: 371 QRYAVNSASFYPADTPLKLVDYFKIDGVYKPGSISDQPTNGAIFPTTSVMQADFRAFVEV 430

Query: 421 VFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSAIYV 480
           +F+N+ED++QSWHLDGYSF+VVGM+ G+W+  SR  YNL DAI RCT+QVYP+SW+AIY+
Sbjct: 431 IFENSEDIVQSWHLDGYSFYVVGMELGKWSPASRKVYNLNDAILRCTIQVYPRSWTAIYI 490

Query: 481 ALDNVGMWNIRTEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRAT 533
           ALDNVGMWN+R+E W RQYLGQQFY+RVYT STSLRDEY IPKNALLCGRA+
Sbjct: 491 ALDNVGMWNMRSEIWERQYLGQQFYMRVYTTSTSLRDEYLIPKNALLCGRAS 534

BLAST of Cucsa.394150 vs. TAIR10
Match: AT4G38420.1 (AT4G38420.1 SKU5 similar 9)

HSP 1 Score: 768.1 bits (1982), Expect = 3.7e-222
Identity = 362/540 (67.04%), Postives = 446/540 (82.59%), Query Frame = 1

Query: 4   SLLFLFISFFFAIVRAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDN 63
           +++ + IS   + V+A+DPYRFFDW VTYG I PLG+PQ+GILINGQ+PGPDI+SVTNDN
Sbjct: 11  TMMMMTISII-SFVQADDPYRFFDWRVTYGNISPLGIPQRGILINGQYPGPDIYSVTNDN 70

Query: 64  IIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYF 123
           +IINV N LDEPFLLSWNG+Q R+NSY+DGVYGTTCPIPPGKN+TY +Q+KDQIGSF+YF
Sbjct: 71  LIINVHNDLDEPFLLSWNGVQLRKNSYQDGVYGTTCPIPPGKNYTYAIQVKDQIGSFFYF 130

Query: 124 PSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLP 183
           PSL  HKAAGGFGG RILSRPRIPVPFP+PAGD+T LIGDW++ +H  L+A LD G  LP
Sbjct: 131 PSLAVHKAAGGFGGFRILSRPRIPVPFPEPAGDFTFLIGDWFKHDHKVLKAILDRGHKLP 190

Query: 184 LPDGILINGRGPNRTASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVEGTHTLQ 243
           LP G+LING+G +  +SI V +GKTYR RISNVGLQ +LNFRIQGH+MK+VEVEGTHT+Q
Sbjct: 191 LPQGVLINGQGVSYMSSITVHKGKTYRFRISNVGLQHTLNFRIQGHQMKLVEVEGTHTVQ 250

Query: 244 TEFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILSTTAILRYANS----AGSVQ 303
           + ++SLDIHVGQSYSVLVT DQP QD+ IVVS++F    L  ++ + Y+NS    + S  
Sbjct: 251 SMYTSLDIHVGQSYSVLVTMDQPDQDYDIVVSTKFVAKKLLVSSTIHYSNSRHSHSSSAN 310

Query: 304 GPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGMITTTRTIVVANSAGIVDG 363
                 P  ++DWS+ QAR+IRTNLTASGPRPNPQGSYHYG I  +RT+++ +SA +V  
Sbjct: 311 SVHVQQPADELDWSIKQARSIRTNLTASGPRPNPQGSYHYGRIKISRTLILESSAALVKR 370

Query: 364 KQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWP-NGGGIYQDTSVMGADYRAFV 423
           KQRYA+N VSF+P DTPLK+AD+FKI GVF++GSI D P  G G+  +TSVMGA +R F+
Sbjct: 371 KQRYAINGVSFVPGDTPLKLADYFKIKGVFKMGSIPDKPRRGRGMRMETSVMGAHHRDFL 430

Query: 424 EIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSAI 483
           EI+FQN E ++QS+HLDGYSF+VVG D G W+  SR +YNLRDAI+R T QVYP+SW+A+
Sbjct: 431 EIIFQNREKIVQSYHLDGYSFWVVGTDRGTWSKASRREYNLRDAISRSTTQVYPESWTAV 490

Query: 484 YVALDNVGMWNIRTEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRP 539
           YVALDNVGMWN+R+E+WARQYLGQQFYLRVY+P+ SLRDEY +PKNALLCGRA+ + T P
Sbjct: 491 YVALDNVGMWNLRSEYWARQYLGQQFYLRVYSPTHSLRDEYLLPKNALLCGRASNKHTTP 549

BLAST of Cucsa.394150 vs. NCBI nr
Match: gi|449450472|ref|XP_004142986.1| (PREDICTED: L-ascorbate oxidase homolog [Cucumis sativus])

HSP 1 Score: 1109.4 bits (2868), Expect = 0.0e+00
Identity = 539/539 (100.00%), Postives = 539/539 (100.00%), Query Frame = 1

Query: 1   MPSSLLFLFISFFFAIVRAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVT 60
           MPSSLLFLFISFFFAIVRAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVT
Sbjct: 1   MPSSLLFLFISFFFAIVRAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVT 60

Query: 61  NDNIIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSF 120
           NDNIIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSF
Sbjct: 61  NDNIIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSF 120

Query: 121 YYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGS 180
           YYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGS
Sbjct: 121 YYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGS 180

Query: 181 MLPLPDGILINGRGPNRTASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVEGTH 240
           MLPLPDGILINGRGPNRTASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVEGTH
Sbjct: 181 MLPLPDGILINGRGPNRTASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVEGTH 240

Query: 241 TLQTEFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILSTTAILRYANSAGSVQG 300
           TLQTEFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILSTTAILRYANSAGSVQG
Sbjct: 241 TLQTEFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILSTTAILRYANSAGSVQG 300

Query: 301 PPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGMITTTRTIVVANSAGIVDGK 360
           PPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGMITTTRTIVVANSAGIVDGK
Sbjct: 301 PPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGMITTTRTIVVANSAGIVDGK 360

Query: 361 QRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFVEI 420
           QRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFVEI
Sbjct: 361 QRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFVEI 420

Query: 421 VFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSAIYV 480
           VFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSAIYV
Sbjct: 421 VFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSAIYV 480

Query: 481 ALDNVGMWNIRTEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL 540
           ALDNVGMWNIRTEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL
Sbjct: 481 ALDNVGMWNIRTEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL 539

BLAST of Cucsa.394150 vs. NCBI nr
Match: gi|659089051|ref|XP_008445302.1| (PREDICTED: L-ascorbate oxidase homolog [Cucumis melo])

HSP 1 Score: 1085.5 bits (2806), Expect = 0.0e+00
Identity = 528/539 (97.96%), Postives = 534/539 (99.07%), Query Frame = 1

Query: 1   MPSSLLFLFISFFFAIVRAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVT 60
           M SSLLFLFISFF AIV AEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVT
Sbjct: 1   MGSSLLFLFISFF-AIVGAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVT 60

Query: 61  NDNIIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSF 120
           NDNIIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSF
Sbjct: 61  NDNIIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSF 120

Query: 121 YYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGS 180
           YYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGS
Sbjct: 121 YYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGS 180

Query: 181 MLPLPDGILINGRGPNRTASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVEGTH 240
           MLPLPDGILINGRGPNRTASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVEGTH
Sbjct: 181 MLPLPDGILINGRGPNRTASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVEGTH 240

Query: 241 TLQTEFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILSTTAILRYANSAGSVQG 300
           TLQTEFSSLD+HVGQSYSVLVTADQPAQDFYIVVSSRFTTPIL+TTAILRYANSAGSVQG
Sbjct: 241 TLQTEFSSLDVHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYANSAGSVQG 300

Query: 301 PPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGMITTTRTIVVANSAGIVDGK 360
           PPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGMI TTRTIV++NSAGIV+GK
Sbjct: 301 PPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGMINTTRTIVLSNSAGIVNGK 360

Query: 361 QRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFVEI 420
           QRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFVEI
Sbjct: 361 QRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFVEI 420

Query: 421 VFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSAIYV 480
           VFQNNEDLIQSWH DGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSAIYV
Sbjct: 421 VFQNNEDLIQSWHFDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSAIYV 480

Query: 481 ALDNVGMWNIRTEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL 540
           ALDNVGMWNIR+EFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL
Sbjct: 481 ALDNVGMWNIRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL 538

BLAST of Cucsa.394150 vs. NCBI nr
Match: gi|590594804|ref|XP_007017955.1| (SKU5 similar 5 isoform 1 [Theobroma cacao])

HSP 1 Score: 956.1 bits (2470), Expect = 2.7e-275
Identity = 460/542 (84.87%), Postives = 498/542 (91.88%), Query Frame = 1

Query: 1   MPSSL---LFLFISFFFAIVRAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIH 60
           MP +L   LF   +  FAI  A+DPYRFF+WNVTYG IYPLG+ Q GILINGQFPGPDIH
Sbjct: 1   MPLNLAVALFCATASLFAIAGADDPYRFFNWNVTYGDIYPLGVRQTGILINGQFPGPDIH 60

Query: 61  SVTNDNIIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQI 120
           SVTNDN+IINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQ+KDQI
Sbjct: 61  SVTNDNLIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQVKDQI 120

Query: 121 GSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLD 180
           GSFYYFPSL FHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWY++NHT LRA LD
Sbjct: 121 GSFYYFPSLAFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYKSNHTDLRAHLD 180

Query: 181 NGSMLPLPDGILINGRGPNRTASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVE 240
            G  LP PDGILINGRGP   AS NVEQGKTYRLRISNVGLQ+SLNFRIQ H++K+VEVE
Sbjct: 181 LGKKLPFPDGILINGRGPGG-ASFNVEQGKTYRLRISNVGLQNSLNFRIQNHKLKLVEVE 240

Query: 241 GTHTLQTEFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILSTTAILRYANSAGS 300
           GTHTLQT +SS+DIHVGQS SVL TADQPAQD+YIVVS+RFT P+L+TTA LRY+NSAG 
Sbjct: 241 GTHTLQTTYSSIDIHVGQSSSVLFTADQPAQDYYIVVSTRFTNPVLTTTATLRYSNSAGP 300

Query: 301 VQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGMITTTRTIVVANSAGIV 360
           V GPPPGGPTIQIDWSLNQAR+IRTNLTASGPRPNPQGSYHYG+I TTRTI +ANSAG V
Sbjct: 301 VSGPPPGGPTIQIDWSLNQARSIRTNLTASGPRPNPQGSYHYGLINTTRTIRLANSAGQV 360

Query: 361 DGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAF 420
           +GKQRYAVNSVSF+PADTPLK+AD+FKIGGVFRVGSISD P GGGIY DTSVM ADYRAF
Sbjct: 361 NGKQRYAVNSVSFVPADTPLKLADYFKIGGVFRVGSISDNPYGGGIYLDTSVMNADYRAF 420

Query: 421 VEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSA 480
           +EIVFQN+ED++QSWHLDGYSFFVVGMDGGQWT+ SRNQYNLRDAI+RCT QVYPKSW+A
Sbjct: 421 IEIVFQNDEDIVQSWHLDGYSFFVVGMDGGQWTAASRNQYNLRDAISRCTTQVYPKSWTA 480

Query: 481 IYVALDNVGMWNIRTEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTR 540
           IYVALDNVGMWN+R+EFWARQYLGQQFYLRVYT S+SLRDEYPIPKNALLCGRA+GR TR
Sbjct: 481 IYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTDSSSLRDEYPIPKNALLCGRASGRSTR 540

BLAST of Cucsa.394150 vs. NCBI nr
Match: gi|596120874|ref|XP_007221787.1| (hypothetical protein PRUPE_ppa003860mg [Prunus persica])

HSP 1 Score: 954.5 bits (2466), Expect = 7.8e-275
Identity = 449/532 (84.40%), Postives = 490/532 (92.11%), Query Frame = 1

Query: 8   LFISFFFAIVRAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDNIIIN 67
           LF++  F+IV AEDPYRFF+WNVTYG IYPLG+ Q+GILINGQFPGPDIHSVTNDN+IIN
Sbjct: 14  LFLTSLFSIVAAEDPYRFFEWNVTYGDIYPLGVRQKGILINGQFPGPDIHSVTNDNLIIN 73

Query: 68  VFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYFPSLG 127
           VFNSLDEPFL+SWNGIQQRRNS+EDGVYGTTCPIPPG+NFTYILQ+KDQIGSFYYFPSL 
Sbjct: 74  VFNSLDEPFLISWNGIQQRRNSFEDGVYGTTCPIPPGRNFTYILQVKDQIGSFYYFPSLA 133

Query: 128 FHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLPLPDG 187
           FHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWY++NHT+L+A LD G  LP PDG
Sbjct: 134 FHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYKSNHTSLKAHLDAGKKLPFPDG 193

Query: 188 ILINGRGPNRTASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVEGTHTLQTEFS 247
           ILINGRGP    S N EQGKTYRLRISNVGLQ SLNFRIQ H++K+VEVEGTHTLQT +S
Sbjct: 194 ILINGRGPGGF-SFNFEQGKTYRLRISNVGLQHSLNFRIQNHKLKLVEVEGTHTLQTTYS 253

Query: 248 SLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILSTTAILRYANSAGSVQGPPPGGPT 307
           SLD+HVGQSYSVLVTADQP QD+YIV SSRFTTPIL+TT  L Y NSAG V GPPPGGPT
Sbjct: 254 SLDVHVGQSYSVLVTADQPGQDYYIVASSRFTTPILTTTGTLHYTNSAGRVSGPPPGGPT 313

Query: 308 IQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGMITTTRTIVVANSAGIVDGKQRYAVNS 367
           IQ+DWSLNQAR+IRTNLTASGPRPNPQGSYHYG+I TT+T V+ NSAG V+GKQRY VNS
Sbjct: 314 IQVDWSLNQARSIRTNLTASGPRPNPQGSYHYGLINTTKTYVLENSAGQVNGKQRYGVNS 373

Query: 368 VSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFVEIVFQNNED 427
           VSF+PADTPLK+AD+FKIGGVFRVGSISD P GGG+Y DTSV+GADYR FVE VFQNNED
Sbjct: 374 VSFVPADTPLKLADYFKIGGVFRVGSISDRPTGGGLYLDTSVLGADYRTFVEFVFQNNED 433

Query: 428 LIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSAIYVALDNVGM 487
           +IQSWHLDGYSFFVVG+DGGQWT  SR +YNLRDA++RCTVQVYPKSW+AIYVALDNVGM
Sbjct: 434 IIQSWHLDGYSFFVVGLDGGQWTPASRKEYNLRDAVSRCTVQVYPKSWTAIYVALDNVGM 493

Query: 488 WNIRTEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL 540
           WN+RTEFWARQYLGQQ YLRVYTPSTS+RDEYPIPKNALLCGRATGRRTRPL
Sbjct: 494 WNLRTEFWARQYLGQQLYLRVYTPSTSIRDEYPIPKNALLCGRATGRRTRPL 544

BLAST of Cucsa.394150 vs. NCBI nr
Match: gi|729330348|ref|XP_010536445.1| (PREDICTED: L-ascorbate oxidase homolog [Tarenaya hassleriana])

HSP 1 Score: 953.4 bits (2463), Expect = 1.7e-274
Identity = 457/530 (86.23%), Postives = 494/530 (93.21%), Query Frame = 1

Query: 10  ISFFFAIVRAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDNIIINVF 69
           IS  FA   AEDPYRFFDWNVTYG IYPLG+ Q+GILINGQFPGPDIHSVTNDN+IINVF
Sbjct: 15  ISLIFAAA-AEDPYRFFDWNVTYGDIYPLGVRQRGILINGQFPGPDIHSVTNDNLIINVF 74

Query: 70  NSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYFPSLGFH 129
           NSLDEPFL+SWNGIQQRRNS++DGVYGTTCPIPPGKNFTYILQ+KDQIGSFYYFPSL FH
Sbjct: 75  NSLDEPFLISWNGIQQRRNSFQDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFH 134

Query: 130 KAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLPLPDGIL 189
           KAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWY++NHT LR QLD+G+ LPL DGIL
Sbjct: 135 KAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYKSNHTDLRVQLDSGNKLPLADGIL 194

Query: 190 INGRGPNRTASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVEGTHTLQTEFSSL 249
           INGRG    A++NVEQGKTYRLRISNVGLQ+SLNFRIQ H+MKVVEVEGTHTLQT FSSL
Sbjct: 195 INGRGSG--ATLNVEQGKTYRLRISNVGLQNSLNFRIQNHKMKVVEVEGTHTLQTTFSSL 254

Query: 250 DIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILSTTAILRYANSAGSVQGPPPGGPTIQ 309
           D+HVGQSYSVLVTADQPAQD+YIVVSSRFT+P+L+TT +LRY+NSAG V GP PGGPTIQ
Sbjct: 255 DVHVGQSYSVLVTADQPAQDYYIVVSSRFTSPVLTTTGVLRYSNSAGPVSGPIPGGPTIQ 314

Query: 310 IDWSLNQARAIRTNLTASGPRPNPQGSYHYGMITTTRTIVVANSAGIVDGKQRYAVNSVS 369
           +DWSLNQARAIRTNLTASGPRPNPQGSYHYGMI TTRTI +A+SAG V+GKQRYAVNSVS
Sbjct: 315 VDWSLNQARAIRTNLTASGPRPNPQGSYHYGMINTTRTIRLASSAGQVNGKQRYAVNSVS 374

Query: 370 FIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFVEIVFQNNEDLI 429
           F PADTPLK+AD+FKI GVFRVGSISD P GGGIY DTSVM ADYRAFVEIVF+N ED++
Sbjct: 375 FTPADTPLKLADYFKIDGVFRVGSISDHPTGGGIYLDTSVMQADYRAFVEIVFENPEDIV 434

Query: 430 QSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSAIYVALDNVGMWN 489
           QSWHLDGYSFFVVGMDGGQWT  SRN+YNLRDA+ARCTVQVYP SW+AIYVALDNVGMWN
Sbjct: 435 QSWHLDGYSFFVVGMDGGQWTPASRNEYNLRDAVARCTVQVYPNSWTAIYVALDNVGMWN 494

Query: 490 IRTEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL 540
           +R+EFWARQYLGQQFYLRVYT STSLRDEYPIPKNALLCGRA+GRRTRPL
Sbjct: 495 VRSEFWARQYLGQQFYLRVYTTSTSLRDEYPIPKNALLCGRASGRRTRPL 541

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ASOL_BRANA8.9e-17055.17L-ascorbate oxidase homolog OS=Brassica napus GN=Bp10 PE=2 SV=1[more]
ASOL_TOBAC3.9e-16553.70L-ascorbate oxidase homolog OS=Nicotiana tabacum PE=2 SV=1[more]
SKU5_ARATH1.7e-15251.47Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana GN=SKU5 PE=1 SV=1[more]
SKS1_ARATH2.5e-14849.18Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana GN=SKS1 PE=1 SV=1[more]
SKS2_ARATH9.0e-14647.10Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana GN=SKS2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LN97_CUCSA0.0e+00100.00Multicopper oxidase OS=Cucumis sativus GN=Csa_2G348200 PE=4 SV=1[more]
A0A061FD20_THECC1.9e-27584.87SKU5 similar 5 isoform 1 OS=Theobroma cacao GN=TCM_034334 PE=4 SV=1[more]
M5XC21_PRUPE5.5e-27584.40Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003860mg PE=4 SV=1[more]
E5GCD6_CUCME2.1e-27485.39Multicopper oxidase OS=Cucumis melo subsp. melo PE=4 SV=1[more]
A0A0D2QIY7_GOSRA3.3e-27284.60Uncharacterized protein OS=Gossypium raimondii GN=B456_009G189900 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G76160.18.1e-27082.31 SKU5 similar 5[more]
AT1G41830.14.7e-25477.34 SKU5-similar 6[more]
AT1G21850.13.5e-24173.97 SKU5 similar 8[more]
AT1G21860.13.8e-23572.93 SKU5 similar 7[more]
AT4G38420.13.7e-22267.04 SKU5 similar 9[more]
Match NameE-valueIdentityDescription
gi|449450472|ref|XP_004142986.1|0.0e+00100.00PREDICTED: L-ascorbate oxidase homolog [Cucumis sativus][more]
gi|659089051|ref|XP_008445302.1|0.0e+0097.96PREDICTED: L-ascorbate oxidase homolog [Cucumis melo][more]
gi|590594804|ref|XP_007017955.1|2.7e-27584.87SKU5 similar 5 isoform 1 [Theobroma cacao][more]
gi|596120874|ref|XP_007221787.1|7.8e-27584.40hypothetical protein PRUPE_ppa003860mg [Prunus persica][more]
gi|729330348|ref|XP_010536445.1|1.7e-27486.23PREDICTED: L-ascorbate oxidase homolog [Tarenaya hassleriana][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001117Cu-oxidase
IPR008972Cupredoxin
IPR011706Cu-oxidase_2
IPR011707Cu-oxidase_3
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
GO:0005507copper ion binding
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0052546 cell wall pectin metabolic process
biological_process GO:0019852 L-ascorbic acid metabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0052541 plant-type cell wall cellulose metabolic process
biological_process GO:0016126 sterol biosynthetic process
cellular_component GO:0048046 apoplast
cellular_component GO:0009505 plant-type cell wall
cellular_component GO:0009506 plasmodesma
cellular_component GO:0005575 cellular_component
molecular_function GO:0005507 copper ion binding
molecular_function GO:0008447 L-ascorbate oxidase activity
molecular_function GO:0016491 oxidoreductase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.394150.1Cucsa.394150.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 156..293
score: 3.9
IPR008972CupredoxinGENE3DG3DSA:2.60.40.420coord: 366..532
score: 2.5E-19coord: 23..144
score: 2.8E-20coord: 152..327
score: 4.9
IPR008972CupredoxinunknownSSF49503Cupredoxinscoord: 341..530
score: 4.58E-37coord: 150..331
score: 1.2E-41coord: 18..170
score: 1.77
IPR011706Multicopper oxidase, type 2PFAMPF07731Cu-oxidase_2coord: 376..511
score: 1.6
IPR011707Multicopper oxidase, type 3PFAMPF07732Cu-oxidase_3coord: 30..143
score: 5.0
NoneNo IPR availablePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 5..538
score:
NoneNo IPR availablePANTHERPTHR11709:SF90MULTI-COPPER OXIDASE TYPE I FAMILY PROTEIN-RELATEDcoord: 5..538
score: