Cucsa.388580 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.388580
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Hyoscyamine 6-dioxygenase
Location	scaffold03967 : 960256 .. 963087 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCAAATGAACTTTGTGTCCAACTGGGAGGGTGTTGAATCAGTACCTGAAAGTTATGTTTGGCCGCAGGAGAAGAGGCCGGGCATTGCACTTAAGAAGACGATTCCCGTGCTTGATTTTGCAACACAGGATCGAGCTCTTCTCTTCCAAAAAATTCTCGACAGTACTCACGAGTTCGGAGTTTTTCAGGTTTGAAAGAGCTCATGAATCCCTTTTTTTTTTCCAAACAACGACAGTTTGTGTGATCTTCTAACTATCTTAAATTTTGATTTTAAACTTTTTAGTTTTTATCGTGGGGATATCAAATCTTTGATTTCGAGGTTGTGGAGTATAGTACTATATGGTGATATATAGTTGAACTATGTTTATATTGATGCTAAATTTTGACGAATGAAAATTATGAAAAATGCACTTGACCCTCACTTGATCCTCACATGATACCAATAAGATAATTATAGAAGTGGGAAAAGGTCAATAAGATAATCATGGATGAGATGAGAAGTTAATACAAGATAATTATGGATGACATTTTGTGGCCGTCAATTTGTTATAGGTGATAAACCATGGAGTATCAAAGAAATTAGTAGAAGAGACAATGAGTGTTTTTAAGGAGTTTCATTCGATGCCACCACAAGGTAAAAGAAGGGAAGTTTCCAAAGACAATAAAAGCTGCAAAGTTTTCACAAGCTCAAACAATTATGAGAATGAACAAACACACTTGTGGAGAGATTGCTTGAGTTTAACTTGTTATCCTGATCTGCATCAAAACATTCATTCTTGGCCTCAAAATCCTCCTAAATTAAGGTAATTAATTTTAAAATAGCATGAAATAATTCTCTTCTAATTAGTTTACCTCATGAGCTAATAATATATATTTTTGTACAACATTTTGATCTATGCCCTAAAAGTTGTTCTCCAAACATGAAATAATTAATATTGTTTTTCTTTTCTTTATTTGTTTATAATATAGTATGATGTTATTGTTTATTAGTAATAATAAATGTATTATGATTGGTTGTTTATGAATATTATAGACACTAATAAATTATTTCCATAAAATATCTATAATATTTCTAAAACTAACTATTCAACTACGTAGGGTAACATTATTTGTTGATAAACAAAATATACAATGAGGATCAAGATTTTGAATTTACCTATATAATCGATTACAATCAATCAATATGCATCAATATTGAGTGTCTGATCATTTTAGTATTATAGTAACGTTACCAAACACTTTAATAACCGTTAAATTATTGTTTGATCCAAAAGTACGTTTAAGTTGTGGGATAAAGATAAATTTAATATCATATTATCTAACTACAACCAACAAAACTTAAAATGATGATTATTATTATTGCAGAGAGGTGGTCGGAGCGTATTGTATTGCAATGGAAAAGTTTTCTCGAGAGATAATAGATCTGATAAGTGAAGGGTTGGGCCTTGGGCAAGGCTACTTCGAGGGAGAAATGAGTTCGTACCGAAGGTTAACAGCAAACCACTATCCAATATGCCCAAACCCTAGTTTGACTATGGGATTGAATCAACATTGCGATCGGGACCTCATGACCATCTTGTTTCAAGATGTCAGTGGTCTTCAAGTCTTCAAGGATGGCCATTGGATTTCTATAGATCCTATTGATGATGCATTTGTGGTTAACTTTGGCTACCTTTTAGAGGTTTGTTAATAATTCACGAAGAATTTTTAAAAATAGTAGATTTTACAAAATATTTACAATTTATAGCAAATTATATCACTCATAATCATTGATATGCTATCGTGATAGAATCTAAAATTTTGCTGTAAATATCTTATTTTATTTTACTATTTTAAAAAATGTCTCTTATTTTAATGGTATCTCGGCTTTCAATTTAGTTTTCATTTTGTTAAAATTATTTTTAGTAGTGGTGAAAAAAATTAAATTATTCATACCAAACCATTTCAATTAATATTATATGTATAATTTGATATGATAGGTTTCACTGAATAGAGAATAAAATTTATTTAGTATATTTTATAAAAGAAAAACCACTGTCACCCTGTCTCAATTTTTCACTTTAAATTTTTAGTTGTATCGAATAATCGCTAGATTTAACTAAGCGGTATTTTTAAAAATAGCAAAATAAATTAAAATATTTACAAACTGTAGTAAAATTTTGAATTCTATCACTATAGCATATTAATTATTAGAGTGATTGCAATTTTTACCCTTGATTTAATTTTTACTAGCTAATATAAAAAAAATTATTTTAATAAAAAATATCTACAAACTCACTAGCTTCTATACTATTAAGATTTTCAAAACTTCAAGTTTGATTATTCAACAAACTAATTAATGAAGTAAACTCCACAAAAAAATTGCAAATTTTAATGATAGAATATACTTATGTTCGTTTATATAATTAATTTTAGTGTTTGTATTGTCATTAAAAACCCTTTTGTTTTTATGTATAAAAAGTATTAATATAGTCCCAACCAAGTCATAGAAAGCACTTTAAAATACTTTTTGGTGAAGCAATATTTATTCTAATACTATGATCTATACAAGTAGATTTTGTGTGATTACCTTATAATATATATAGGTCATTAGCAATGGAAAGCTCAAGGCAGTAGAACATCGAGTCGTGACAAACGCAGAGGCATCTCGACAAAGCTTTGGGTATGTTATTTTTCCTGAGAAAGAGATGATAATAGAACCAGCAAAATGTTTGATAAATGAAGCCAATCCTCCACATTATCGTTCCTTTAAGGTTGAAGACTTCAATAGGCATTTTTTTGCTGTGTCAAATAATAATGAGGAAACAATGAAGTTTATATTAGCATAA

mRNA sequence

ATGGCTCAAATGAACTTTGTGTCCAACTGGGAGGGTGTTGAATCAGTACCTGAAAGTTATGTTTGGCCGCAGGAGAAGAGGCCGGGCATTGCACTTAAGAAGACGATTCCCGTGCTTGATTTTGCAACACAGGATCGAGCTCTTCTCTTCCAAAAAATTCTCGACAGTACTCACGAGTTCGGAGTTTTTCAGGTGATAAACCATGGAGTATCAAAGAAATTAGTAGAAGAGACAATGAGTGTTTTTAAGGAGTTTCATTCGATGCCACCACAAGGTAAAAGAAGGGAAGTTTCCAAAGACAATAAAAGCTGCAAAGTTTTCACAAGCTCAAACAATTATGAGAATGAACAAACACACTTGTGGAGAGATTGCTTGAGTTTAACTTGTTATCCTGATCTGCATCAAAACATTCATTCTTGGCCTCAAAATCCTCCTAAATTAAGAGAGGTGGTCGGAGCGTATTGTATTGCAATGGAAAAGTTTTCTCGAGAGATAATAGATCTGATAAGTGAAGGGTTGGGCCTTGGGCAAGGCTACTTCGAGGGAGAAATGAGTTCGTACCGAAGGTTAACAGCAAACCACTATCCAATATGCCCAAACCCTAGTTTGACTATGGGATTGAATCAACATTGCGATCGGGACCTCATGACCATCTTGTTTCAAGATGTCAGTGGTCTTCAAGTCTTCAAGGATGGCCATTGGATTTCTATAGATCCTATTGATGATGCATTTGTGGTTAACTTTGGCTACCTTTTAGAGGTCATTAGCAATGGAAAGCTCAAGGCAGTAGAACATCGAGTCGTGACAAACGCAGAGGCATCTCGACAAAGCTTTGGGTATGTTATTTTTCCTGAGAAAGAGATGATAATAGAACCAGCAAAATGTTTGATAAATGAAGCCAATCCTCCACATTATCGTTCCTTTAAGGTTGAAGACTTCAATAGGCATTTTTTTGCTGTGTCAAATAATAATGAGGAAACAATGAAGTTTATATTAGCATAA

Coding sequence (CDS)

ATGGCTCAAATGAACTTTGTGTCCAACTGGGAGGGTGTTGAATCAGTACCTGAAAGTTATGTTTGGCCGCAGGAGAAGAGGCCGGGCATTGCACTTAAGAAGACGATTCCCGTGCTTGATTTTGCAACACAGGATCGAGCTCTTCTCTTCCAAAAAATTCTCGACAGTACTCACGAGTTCGGAGTTTTTCAGGTGATAAACCATGGAGTATCAAAGAAATTAGTAGAAGAGACAATGAGTGTTTTTAAGGAGTTTCATTCGATGCCACCACAAGGTAAAAGAAGGGAAGTTTCCAAAGACAATAAAAGCTGCAAAGTTTTCACAAGCTCAAACAATTATGAGAATGAACAAACACACTTGTGGAGAGATTGCTTGAGTTTAACTTGTTATCCTGATCTGCATCAAAACATTCATTCTTGGCCTCAAAATCCTCCTAAATTAAGAGAGGTGGTCGGAGCGTATTGTATTGCAATGGAAAAGTTTTCTCGAGAGATAATAGATCTGATAAGTGAAGGGTTGGGCCTTGGGCAAGGCTACTTCGAGGGAGAAATGAGTTCGTACCGAAGGTTAACAGCAAACCACTATCCAATATGCCCAAACCCTAGTTTGACTATGGGATTGAATCAACATTGCGATCGGGACCTCATGACCATCTTGTTTCAAGATGTCAGTGGTCTTCAAGTCTTCAAGGATGGCCATTGGATTTCTATAGATCCTATTGATGATGCATTTGTGGTTAACTTTGGCTACCTTTTAGAGGTCATTAGCAATGGAAAGCTCAAGGCAGTAGAACATCGAGTCGTGACAAACGCAGAGGCATCTCGACAAAGCTTTGGGTATGTTATTTTTCCTGAGAAAGAGATGATAATAGAACCAGCAAAATGTTTGATAAATGAAGCCAATCCTCCACATTATCGTTCCTTTAAGGTTGAAGACTTCAATAGGCATTTTTTTGCTGTGTCAAATAATAATGAGGAAACAATGAAGTTTATATTAGCATAA

Protein sequence

MAQMNFVSNWEGVESVPESYVWPQEKRPGIALKKTIPVLDFATQDRALLFQKILDSTHEFGVFQVINHGVSKKLVEETMSVFKEFHSMPPQGKRREVSKDNKSCKVFTSSNNYENEQTHLWRDCLSLTCYPDLHQNIHSWPQNPPKLREVVGAYCIAMEKFSREIIDLISEGLGLGQGYFEGEMSSYRRLTANHYPICPNPSLTMGLNQHCDRDLMTILFQDVSGLQVFKDGHWISIDPIDDAFVVNFGYLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCLINEANPPHYRSFKVEDFNRHFFAVSNNNEETMKFILA*

Homology

[Close]

BLAST of Cucsa.388580 vs. Swiss-Prot
Match: HY6H_HYONI (Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger GN=H6H PE=1 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 3.2e-61
Identity = 127/321 (39.56%), Postives = 187/321 (58.26%), Query Frame = 1


Query: 6   FVSNWEGVESVPESYVWPQEKRP--GIALKKTIPVLDFATQDRALLFQKILDSTHEFGVF 65
           FVSNW   +SV ES++ P +KR    + +   +P++D   Q   LL Q+I  +  +FG+F
Sbjct: 4   FVSNWS-TKSVSESFIAPLQKRAEKDVPVGNDVPIIDLQ-QHHHLLVQQITKACQDFGLF 63

Query: 66  QVINHGVSKKLVEETMSVFKEFHSMPPQGKRREVSKDN---------KSCKVFTSSNNYE 125
           QVINHG  ++L+ ETM V KEF ++P + K +   K           +  K++       
Sbjct: 64  QVINHGFPEELMLETMEVCKEFFALPAEEKEKFKPKGEAAKFELPLEQKAKLYVEGEQLS 123

Query: 126 NEQTHLWRDCLSLTCYPDLHQNIHSWPQNPPKLREVVGAYCIAMEKFSREIIDLISEGLG 185
           NE+   W+D L+  C+P     ++SWP+ P K REVV  Y + + K +  ++D I EGLG
Sbjct: 124 NEEFLYWKDTLAHGCHPLDQDLVNSWPEKPAKYREVVAKYSVEVRKLTMRMLDYICEGLG 183

Query: 186 LGQGYFEGEMSSYRRLTANHYPICPNPSLTMGLNQHCDRDLMTILFQDVSGLQ--VFKDG 245
           L  GYF+ E+S  + +  N+YP CP+PS T+G   H D +L+T+L QD+ GLQ  + KD 
Sbjct: 184 LKLGYFDNELSQIQMMLTNYYPPCPDPSSTLGSGGHYDGNLITLLQQDLPGLQQLIVKDA 243

Query: 246 HWISIDPIDDAFVVNFGYLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEP 305
            WI++ PI  AFVVN G  L+VI+N K +   HRVVT+    R S   +I P+    IEP
Sbjct: 244 TWIAVQPIPTAFVVNLGLTLKVITNEKFEGSIHRVVTDPTRDRVSIATLIGPDYSCTIEP 303

Query: 306 AKCLINEANPPHYRSFKVEDF 314
           AK L+N+ NPP Y+ +   +F
Sbjct: 304 AKELLNQDNPPLYKPYSYSEF 322

[Close]

BLAST of Cucsa.388580 vs. Swiss-Prot
Match: DMR6_ARATH (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana GN=DMR6 PE=1 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 2.4e-56
Identity = 120/327 (36.70%), Postives = 188/327 (57.49%), Query Frame = 1


Query: 15  SVPESYVWPQEKRPG---IALKKTIPVLDFATQDRALLFQKILDSTHEFGVFQVINHGVS 74
           ++PE+YV P   RP    ++  +  P++D ++ DR+ L Q+I  +   FG FQVINHGV+
Sbjct: 14  TLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFGFFQVINHGVN 73

Query: 75  KKLVEETMSVFKEFHSMPPQGKRREVSKDNKSCKVFTSSNNYENEQTHLWRDCLSLTCYP 134
           K++++E +SV +EF SM  + K +  S D       ++S N + E+ + WRD L L CYP
Sbjct: 74  KQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSFNVKKEEVNNWRDYLRLHCYP 133

Query: 135 DLHQNIHSWPQNPPKLREVVGAYCIAMEKFSREIIDLISEGLGLGQGYFEGEMSSY-RRL 194
            +H+ ++ WP NPP  +E+V  Y   + +   +I +LISE LGL + Y +  +    + +
Sbjct: 134 -IHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQGQHM 193

Query: 195 TANHYPICPNPSLTMGLNQHCDRDLMTILFQD--VSGLQVFKDGHWISIDPIDDAFVVNF 254
             N+YP CP P LT GL  H D + +TIL QD  V GLQ+  DG W +++P  DAFV+N 
Sbjct: 194 AVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAFVINI 253

Query: 255 GYLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCL---INEANPPHY 314
           G  L+ +SNG  K+V HR VTN E  R S    + P    ++ PAK L    ++   P Y
Sbjct: 254 GDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDDETKPVY 313

Query: 315 RSFKVEDFNRHFFAVSNNNEETMKFIL 333
           + F   ++ + F++ + + E  ++  L
Sbjct: 314 KDFTYAEYYKKFWSRNLDQEHCLENFL 339

[Close]

BLAST of Cucsa.388580 vs. Swiss-Prot
Match: IDS3_HORVU (2'-deoxymugineic-acid 2'-dioxygenase OS=Hordeum vulgare GN=IDS3 PE=1 SV=3)

HSP 1 Score: 219.9 bits (559), Expect = 4.1e-56
Identity = 116/309 (37.54%), Postives = 177/309 (57.28%), Query Frame = 1


Query: 15  SVPESYVWPQEK-RPGIALKKTIPVLDFATQDRALLFQKILDSTHEFGVFQVINHGVSKK 74
           S+PES+V+  +K  P      ++P++D +   R  + + IL++  E G FQV+NHGVSK+
Sbjct: 13  SLPESFVFASDKVPPATKAVVSLPIIDLSC-GRDEVRRSILEAGKELGFFQVVNHGVSKQ 72

Query: 75  LVEETMSVFKEFHSMPPQGKRREVSKDNKSCKVFTSSNNYENEQTHLWRDCLSLTCYPDL 134
           ++ +   + ++F  +P   K    S++        S   Y+      WRDCL L C   +
Sbjct: 73  VMRDMEGMCEQFFHLPAADKASLYSEERHKPNRLFSGATYDTGGEKYWRDCLRLACPFPV 132

Query: 135 HQNIHSWPQNPPKLREVVGAYCIAMEKFSREIIDLISEGLGLGQGYFEGEMSSYRR-LTA 194
             +I+ WP  P  LR+V+  +        +E++ L+ EG+G+   YFEG++S     L  
Sbjct: 133 DDSINEWPDTPKGLRDVIEKFTSQTRDVGKELLRLLCEGMGIQADYFEGDLSGGNVILNI 192

Query: 195 NHYPICPNPSLTMGLNQHCDRDLMTILFQD-VSGLQVFKDGHWISIDPIDDAFVVNFGYL 254
           NHYP CPNP   +G   HCDR+L+T+L    V+GL+V   G WI +DP  +AFVVNFG  
Sbjct: 193 NHYPSCPNPDKALGQPPHCDRNLITLLLPGAVNGLEVSYKGDWIKVDPAPNAFVVNFGQQ 252

Query: 255 LEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCLINEANPPHYRSFKVE 314
           LEV++NG LK++EHR +TN+  +R S    I P +E +I PAK  +++ NPP YR+    
Sbjct: 253 LEVVTNGLLKSIEHRAMTNSALARTSVATFIMPTQECLIGPAKEFLSKENPPCYRTTMFR 312

Query: 315 DFNRHFFAV 321
           DF R +  V
Sbjct: 313 DFMRIYNVV 320

[Close]

BLAST of Cucsa.388580 vs. Swiss-Prot
Match: IDS2_HORVU (Mugineic-acid 3-dioxygenase OS=Hordeum vulgare GN=IDS2 PE=2 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 3.8e-54
Identity = 117/303 (38.61%), Postives = 169/303 (55.78%), Query Frame = 1


Query: 15  SVPESYVWPQEKRPGIALKKTIPVLDFATQDRALLFQKILDSTHEFGVFQVINHGVSKKL 74
           S+P+S++ P ++        ++P++D + + R  + Q ILDS  E+G  QV+NHG+S+ +
Sbjct: 14  SIPDSFLLPADRLHPATTDVSLPIIDMS-RGRDEVRQAILDSGKEYGFIQVVNHGISEPM 73

Query: 75  VEETMSVFKEFHSMPPQGKRREVSKDNKSCKVFTSSNNYENEQTHLWRDCLSLTCYPDLH 134
           + E  +V  EF  MP + K    S+D          + +E      W D L L  YP   
Sbjct: 74  LHEMYAVCHEFFDMPAEDKAEFFSEDRSERNKLFCGSAFETLGEKYWIDVLELL-YPLPS 133

Query: 135 QNIHSWPQNPPKLREVVGAYCIAMEKFSREIIDLISEGLGLGQGYFEGEMSSYRRLT-AN 194
            +   WP  P  LREVVG Y       + EI+ L+ EGLGL   +F G++S  R +   N
Sbjct: 134 GDTKDWPHKPQMLREVVGNYTSLARGVAMEILRLLCEGLGLRPDFFVGDISGGRVVVDIN 193

Query: 195 HYPICPNPSLTMGLNQHCDRDLMTILFQD-VSGLQVFKDGHWISIDPIDDAFVVNFGYLL 254
           +YP  PNPS T+GL  HCDRDLMT+L    V GL++   G WI + P+ ++ V+NFG  L
Sbjct: 194 YYPPSPNPSRTLGLPPHCDRDLMTVLLPGAVPGLEIAYKGGWIKVQPVPNSLVINFGLQL 253

Query: 255 EVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCLINEANPPHYRSFKVED 314
           EV++NG LKAVEHR  TN    R S    I P  + ++ PA+  ++E NPP YR+  V +
Sbjct: 254 EVVTNGYLKAVEHRAATNFAEPRLSVASFIVPADDCVVGPAEEFVSEDNPPRYRTLTVGE 313

Query: 315 FNR 316
           F R
Sbjct: 314 FKR 314

[Close]

BLAST of Cucsa.388580 vs. Swiss-Prot
Match: DLO2_ARATH (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana GN=DLO2 PE=2 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 2.8e-49
Identity = 113/324 (34.88%), Postives = 175/324 (54.01%), Query Frame = 1


Query: 13  VESVPESYVWPQEKRPGIALKKT----IPVLDFAT---QDRALLFQKILDSTHEFGVFQV 72
           V+ VP +YV P   RP ++  +T    IP++D       +RA +  +   +    G FQ+
Sbjct: 15  VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQI 74

Query: 73  INHGVSKKLVEETMSVFKEFHSMPPQGKRREVSKDNKSCKVFTSSNNYENEQTHLWRDCL 132
            NHGV ++ +++ M+  +EF       + +  S D K     ++S N   E+   WRD L
Sbjct: 75  KNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSFNVSKEKVSNWRDFL 134

Query: 133 SLTCYPDLHQNIHSWPQNPPKLREVVGAYCIAMEKFSREIIDLISEGLGLGQGYFEGEMS 192
            L CYP +   I+ WP  P   REV   Y  ++      +++ ISE LGL +      + 
Sbjct: 135 RLHCYP-IEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIG 194

Query: 193 SY-RRLTANHYPICPNPSLTMGLNQHCDRDLMTILFQD-VSGLQVFKDGHWISIDPIDDA 252
            + + +  N+YP CP P LT GL  H D +L+T+L QD VSGLQVFKDG WI+++P+ + 
Sbjct: 195 KHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNT 254

Query: 253 FVVNFGYLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCLINEA--N 312
           F+VN G  ++VISN K K+V HR V N++  R S      P ++ +I PA+ LINE   +
Sbjct: 255 FIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDS 314

Query: 313 PPHYRSFKVEDFNRHFFAVSNNNE 326
           P  YR+F   ++   F+  + + E
Sbjct: 315 PAIYRNFTYAEYFEKFWDTAFDTE 337

[Close]

BLAST of Cucsa.388580 vs. TrEMBL
Match: A0A0A0K4U9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G376360 PE=3 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 1.6e-195
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 1


Query: 1   MAQMNFVSNWEGVESVPESYVWPQEKRPGIALKKTIPVLDFATQDRALLFQKILDSTHEF 60
           MAQMNFVSNWEGVESVPESYVWPQEKRPGIALKKTIPVLDFATQDRALLFQKILDSTHEF
Sbjct: 1   MAQMNFVSNWEGVESVPESYVWPQEKRPGIALKKTIPVLDFATQDRALLFQKILDSTHEF 60

Query: 61  GVFQVINHGVSKKLVEETMSVFKEFHSMPPQGKRREVSKDNKSCKVFTSSNNYENEQTHL 120
           GVFQVINHGVSKKLVEETMSVFKEFHSMPPQGKRREVSKDNKSCKVFTSSNNYENEQTHL
Sbjct: 61  GVFQVINHGVSKKLVEETMSVFKEFHSMPPQGKRREVSKDNKSCKVFTSSNNYENEQTHL 120

Query: 121 WRDCLSLTCYPDLHQNIHSWPQNPPKLREVVGAYCIAMEKFSREIIDLISEGLGLGQGYF 180
           WRDCLSLTCYPDLHQNIHSWPQNPPKLREVVGAYCIAMEKFSREIIDLISEGLGLGQGYF
Sbjct: 121 WRDCLSLTCYPDLHQNIHSWPQNPPKLREVVGAYCIAMEKFSREIIDLISEGLGLGQGYF 180

Query: 181 EGEMSSYRRLTANHYPICPNPSLTMGLNQHCDRDLMTILFQDVSGLQVFKDGHWISIDPI 240
           EGEMSSYRRLTANHYPICPNPSLTMGLNQHCDRDLMTILFQDVSGLQVFKDGHWISIDPI
Sbjct: 181 EGEMSSYRRLTANHYPICPNPSLTMGLNQHCDRDLMTILFQDVSGLQVFKDGHWISIDPI 240

Query: 241 DDAFVVNFGYLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCLINEA 300
           DDAFVVNFGYLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCLINEA
Sbjct: 241 DDAFVVNFGYLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCLINEA 300

Query: 301 NPPHYRSFKVEDFNRHFFAVSNNNEETMKFILA 334
           NPPHYRSFKVEDFNRHFFAVSNNNEETMKFILA
Sbjct: 301 NPPHYRSFKVEDFNRHFFAVSNNNEETMKFILA 333

[Close]

BLAST of Cucsa.388580 vs. TrEMBL
Match: A0A0A0K5H3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G378370 PE=3 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 9.3e-116
Identity = 200/330 (60.61%), Postives = 254/330 (76.97%), Query Frame = 1


Query: 6   FVSNWEGVESVPESYVWPQEKRPG---IALKKTIPVLDFATQDRALLFQKILDSTHEFGV 65
           FVSNWE VESVPESYV+P EKRPG   + + K IPV+D + +DR LL +KILD++ EFG 
Sbjct: 8   FVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEFGF 67

Query: 66  FQVINHGVSKKLVEETMSVFKEFHSMPPQGKRREVSKD-NKSCKVFTSSNNYENEQTHLW 125
           FQ+INHGVSKK+ EETM +FKEFH+M    K +E SKD N+SC+V+TSS NY  EQ H W
Sbjct: 68  FQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQVHCW 127

Query: 126 RDCLSLTCYPDLHQNIHSWPQNPPKLREVVGAYCIAMEKFSREIIDLISEGLGLGQGYFE 185
           RD L   C+P L + +H WPQ PPK REVVGAYC+AM K   EI++L+SEGLGLG+GYF 
Sbjct: 128 RDALIFNCHP-LEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYFG 187

Query: 186 GEMSSYRRLTANHYPICPNPSLTMGLNQHCDRDLMTILFQDVSGLQVFKDGHWISIDPID 245
           GEMS    L  NHYP CPNPSLT+GL+QHCD  L+TILFQDV+GLQV KDG WI + PID
Sbjct: 188 GEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQPID 247

Query: 246 DAFVVNFGYLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCLINEAN 305
           +AFVVN G++L++I+NGKL+A EHR VTN++ SRQS  Y+++P+ E ++EPAKC+INEAN
Sbjct: 248 NAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEAN 307

Query: 306 PPHYRSFKVEDFNRHFFAVSNNNEETMKFI 332
           PP YRS   +DF R++   + + +  M++I
Sbjct: 308 PPRYRSLNFKDFQRNYLPRAVDTKAVMQYI 336

[Close]

BLAST of Cucsa.388580 vs. TrEMBL
Match: A0A0A0K5H0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G375850 PE=3 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 3.5e-115
Identity = 201/332 (60.54%), Postives = 253/332 (76.20%), Query Frame = 1


Query: 1   MAQMNFVSNWEGVESVPESYVWPQEKRPGIALKKTIPVLDFATQDRALLFQKILDSTHEF 60
           M++  FVSNW GV SVPE+YV+P EKR GI++KK+IPV+D++  DRALL +++ D T EF
Sbjct: 2   MSRTKFVSNWGGVVSVPETYVYPPEKRFGISVKKSIPVIDWS-DDRALLLKRVHDVTQEF 61

Query: 61  GVFQVINHGVSKKLVEETMSVFKEFHSMPPQGKRREVSKD-NKSCKVFTSSNNYENEQTH 120
           G  QVINHGV K LVEE M + KEFH M  + K RE SKD +KSCKV+TSS NYENEQ H
Sbjct: 62  GFVQVINHGVPKTLVEEMMKIMKEFHGMSAEDKERECSKDPDKSCKVYTSSQNYENEQIH 121

Query: 121 LWRDCLSLTCYPDLHQNIHSWPQNPPKLREVVGAYCIAMEKFSREIIDLISEGLGLGQGY 180
           LW+D   L C+P+L +N+  WPQNPPKLREVV AY + +EKF+ EI++++SEGLGL +GY
Sbjct: 122 LWKDTFKLRCHPNLDKNLEFWPQNPPKLREVVKAYIVMIEKFASEILEVLSEGLGLEKGY 181

Query: 181 FEGEMSSYRRLTANHYPICPNPSLTMGLNQHCDRDLMTILFQDVSGLQVFKDGHWISIDP 240
           F GEMS      A+HYP CPNPSLT+GL+QH D  L+TILFQDV+GLQ  KDG WI +DP
Sbjct: 182 FRGEMSKNPEFLAHHYPPCPNPSLTVGLSQHADPTLITILFQDVNGLQFLKDGQWIDVDP 241

Query: 241 IDDAFVVNFGYLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCLINE 300
           + DAF+VN GY+ EVISNGKLKA EHRVVTN+  SRQ+  YV++PE ++ IEP+KC INE
Sbjct: 242 LPDAFLVNLGYVFEVISNGKLKAAEHRVVTNSTTSRQTLVYVVYPENDVTIEPSKCFINE 301

Query: 301 ANPPHYRSFKVEDFNRHFFAVSNNNEETMKFI 332
           ANPPHYRS + +DF     +   + +  M+FI
Sbjct: 302 ANPPHYRSLEFKDFYNKLMSKPPDRKTAMEFI 332

[Close]

BLAST of Cucsa.388580 vs. TrEMBL
Match: A0A0A0K745_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G375860 PE=3 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 2.3e-114
Identity = 197/332 (59.34%), Postives = 250/332 (75.30%), Query Frame = 1


Query: 1   MAQMNFVSNWEGVESVPESYVWPQEKRPGIALKKTIPVLDFATQDRALLFQKILDSTHEF 60
           M+Q  FVSNW+G+ SVPE+YV P EKR G+++KK IPV+D AT+DR LL +K+LD + EF
Sbjct: 1   MSQTKFVSNWKGIGSVPETYVCPPEKRVGVSVKKKIPVIDLATEDRDLLSKKVLDVSQEF 60

Query: 61  GVFQVINHGVSKKLVEETMSVFKEFHSMPPQGKRREVSKD-NKSCKVFTSSNNYENEQTH 120
           G FQVINHGVSK LVEETM V +EFH+M  + K  E SKD NKSC +++S+ NYE E+ H
Sbjct: 61  GFFQVINHGVSKALVEETMRVSREFHAMSSEDKEMECSKDPNKSCLIYSSTPNYEKEELH 120

Query: 121 LWRDCLSLTCYPDLHQNIHSWPQNPPKLREVVGAYCIAMEKFSREIIDLISEGLGLGQGY 180
           LW+D L L CYP+L++N+  WPQNPPK REV+ AY + M+KFS EI++++SEGLGL + Y
Sbjct: 121 LWKDSLRLICYPNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLEKEY 180

Query: 181 FEGEMSSYRRLTANHYPICPNPSLTMGLNQHCDRDLMTILFQDVSGLQVFKDGHWISIDP 240
           F G MS+      +HYP CPNPSLT+GLN+H D  ++TILFQDV+GLQ  KDG WI +DP
Sbjct: 181 FSGGMSNNPEFLVHHYPPCPNPSLTLGLNKHADPTVITILFQDVNGLQFLKDGEWIDVDP 240

Query: 241 IDDAFVVNFGYLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCLINE 300
           + DAF+VN GY+ EVISNGK+KA EHRVVTN   SRQS  Y ++PE  M IEP+KC INE
Sbjct: 241 LPDAFLVNLGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCFINE 300

Query: 301 ANPPHYRSFKVEDFNRHFFAVSNNNEETMKFI 332
           ANPPHYR+ + +DF     +   + E  MKFI
Sbjct: 301 ANPPHYRTLEFKDFVNKLRSKHPDREMAMKFI 332

[Close]

BLAST of Cucsa.388580 vs. TrEMBL
Match: A0A061DWT9_THECC (2-oxoglutarate and Fe(II)-dependent oxygenase superfamily protein, putative OS=Theobroma cacao GN=TCM_004072 PE=3 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 3.8e-85
Identity = 160/335 (47.76%), Postives = 220/335 (65.67%), Query Frame = 1


Query: 4   MNFVSNWEGVESVPESYVWPQEKRPG---IALKKTIPVLDFATQDRALLFQKILDSTHEF 63
           M  +S+W    S+PESYV P E RPG   + L ++IPV+D    DR    Q+IL +  E+
Sbjct: 1   MELLSSWCYDGSLPESYVMPPESRPGKLIVPLGQSIPVVDLERHDRNETIQQILKAGEEY 60

Query: 64  GVFQVINHGVSKKLVEETMSVFKEFHSMPPQGKRREVSKD-NKSCKVFTSSNNYENEQTH 123
           G FQVINHGVS+ L+++TM+V +EFH+MP   K RE SKD +  CK++TSS  Y  E  H
Sbjct: 61  GFFQVINHGVSEDLMDKTMNVAEEFHAMPAVDKERECSKDPSGGCKLYTSSYVYPKEDFH 120

Query: 124 LWRDCLSLTCYPDLHQNIHSWPQNPPKLREVVGAYCIAMEKFSREIIDLISEGLGLGQGY 183
            WRD ++  C P L + I  WP+ P + REVVGAY + + K S  I++LI EGLGL  GY
Sbjct: 121 YWRDAMTHPCLP-LEEYIQFWPEKPTRYREVVGAYSVELRKLSCRILELICEGLGLSAGY 180

Query: 184 FEGEMSSYRRLTANHYPICPNPSLTMGLNQHCDRDLMTILFQD-VSGLQVFKDGHWISID 243
           F  ++    ++  NHYP CP PSLT+GL++H D  ++TIL Q  + GLQVFKDGHWI ++
Sbjct: 181 FSSDLIQAPKIMINHYPPCPEPSLTLGLSKHRDPSIITILLQGHIHGLQVFKDGHWIGVE 240

Query: 244 PIDDAFVVNFGYLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCLIN 303
           P+  AFVVN G+LL++ISNGKLK  EHRVVTN+  +R +  Y ++P  + ++EPAK L+N
Sbjct: 241 PLPHAFVVNIGFLLQIISNGKLKGAEHRVVTNSRDARTTISYFVYPSDQTLVEPAKALVN 300

Query: 304 EANPPHYRSFKVEDFNRHFFAVSNNNEETMKFILA 334
             NP  Y++FK  DF   F     N  E +K +++
Sbjct: 301 ACNPTLYKAFKFFDFVSAFLLKPGNTGEAVKELIS 334

[Close]

BLAST of Cucsa.388580 vs. TAIR10
Match: AT5G24530.1 (AT5G24530.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 220.7 bits (561), Expect = 1.4e-57
Identity = 120/327 (36.70%), Postives = 188/327 (57.49%), Query Frame = 1


Query: 15  SVPESYVWPQEKRPG---IALKKTIPVLDFATQDRALLFQKILDSTHEFGVFQVINHGVS 74
           ++PE+YV P   RP    ++  +  P++D ++ DR+ L Q+I  +   FG FQVINHGV+
Sbjct: 14  TLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFGFFQVINHGVN 73

Query: 75  KKLVEETMSVFKEFHSMPPQGKRREVSKDNKSCKVFTSSNNYENEQTHLWRDCLSLTCYP 134
           K++++E +SV +EF SM  + K +  S D       ++S N + E+ + WRD L L CYP
Sbjct: 74  KQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSFNVKKEEVNNWRDYLRLHCYP 133

Query: 135 DLHQNIHSWPQNPPKLREVVGAYCIAMEKFSREIIDLISEGLGLGQGYFEGEMSSY-RRL 194
            +H+ ++ WP NPP  +E+V  Y   + +   +I +LISE LGL + Y +  +    + +
Sbjct: 134 -IHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQGQHM 193

Query: 195 TANHYPICPNPSLTMGLNQHCDRDLMTILFQD--VSGLQVFKDGHWISIDPIDDAFVVNF 254
             N+YP CP P LT GL  H D + +TIL QD  V GLQ+  DG W +++P  DAFV+N 
Sbjct: 194 AVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAFVINI 253

Query: 255 GYLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCL---INEANPPHY 314
           G  L+ +SNG  K+V HR VTN E  R S    + P    ++ PAK L    ++   P Y
Sbjct: 254 GDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDDETKPVY 313

Query: 315 RSFKVEDFNRHFFAVSNNNEETMKFIL 333
           + F   ++ + F++ + + E  ++  L
Sbjct: 314 KDFTYAEYYKKFWSRNLDQEHCLENFL 339

[Close]

BLAST of Cucsa.388580 vs. TAIR10
Match: AT4G10490.1 (AT4G10490.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 197.2 bits (500), Expect = 1.6e-50
Identity = 113/324 (34.88%), Postives = 175/324 (54.01%), Query Frame = 1


Query: 13  VESVPESYVWPQEKRPGIALKKT----IPVLDFAT---QDRALLFQKILDSTHEFGVFQV 72
           V+ VP +YV P   RP ++  +T    IP++D       +RA +  +   +    G FQ+
Sbjct: 15  VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQI 74

Query: 73  INHGVSKKLVEETMSVFKEFHSMPPQGKRREVSKDNKSCKVFTSSNNYENEQTHLWRDCL 132
            NHGV ++ +++ M+  +EF       + +  S D K     ++S N   E+   WRD L
Sbjct: 75  KNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSFNVSKEKVSNWRDFL 134

Query: 133 SLTCYPDLHQNIHSWPQNPPKLREVVGAYCIAMEKFSREIIDLISEGLGLGQGYFEGEMS 192
            L CYP +   I+ WP  P   REV   Y  ++      +++ ISE LGL +      + 
Sbjct: 135 RLHCYP-IEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIG 194

Query: 193 SY-RRLTANHYPICPNPSLTMGLNQHCDRDLMTILFQD-VSGLQVFKDGHWISIDPIDDA 252
            + + +  N+YP CP P LT GL  H D +L+T+L QD VSGLQVFKDG WI+++P+ + 
Sbjct: 195 KHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNT 254

Query: 253 FVVNFGYLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCLINEA--N 312
           F+VN G  ++VISN K K+V HR V N++  R S      P ++ +I PA+ LINE   +
Sbjct: 255 FIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDS 314

Query: 313 PPHYRSFKVEDFNRHFFAVSNNNE 326
           P  YR+F   ++   F+  + + E
Sbjct: 315 PAIYRNFTYAEYFEKFWDTAFDTE 337

[Close]

BLAST of Cucsa.388580 vs. TAIR10
Match: AT4G10500.1 (AT4G10500.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 185.7 bits (470), Expect = 4.8e-47
Identity = 105/302 (34.77%), Postives = 160/302 (52.98%), Query Frame = 1


Query: 7   VSNWEGVESVPESYVWPQEKRPGIALKKT----IPVLDFAT---QDRALLFQKILDSTHE 66
           VS++     +P +YV P   RP ++  ++    IP++D       +RA++ Q++  +   
Sbjct: 11  VSDFASSVHIPSNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACST 70

Query: 67  FGVFQVINHGVSKKLVEETMSVFKEFHSMPPQGKRREVSKDNKSCKVFTSSNNYENEQTH 126
           +G FQ+ NHGV    V +  +V +EF   P   + +  S D       ++S N   ++  
Sbjct: 71  YGFFQIKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSFNVGADKVL 130

Query: 127 LWRDCLSLTCYPDLHQNIHSWPQNPPKLREVVGAYCIAMEKFSREIIDLISEGLGLGQGY 186
            WRD L L C+P +   I  WP +P   REV   Y  ++      +++ ISE LGL   +
Sbjct: 131 NWRDFLRLHCFP-IEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESDH 190

Query: 187 FEGEMSSY-RRLTANHYPICPNPSLTMGLNQHCDRDLMTILFQD-VSGLQVFKDGHWISI 246
               +  + + +  N+YP CP P LT GL  H D  ++T+L QD VSGLQVFKD  W+++
Sbjct: 191 ISNILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWVAV 250

Query: 247 DPIDDAFVVNFGYLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCLI 300
            PI + F+VN G  ++VISN K K+V HR V N E  R S     FP  + +I PA  L+
Sbjct: 251 SPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHELV 310

[Close]

BLAST of Cucsa.388580 vs. TAIR10
Match: AT5G08640.1 (AT5G08640.1 flavonol synthase 1)

HSP 1 Score: 176.4 bits (446), Expect = 2.9e-44
Identity = 96/311 (30.87%), Postives = 167/311 (53.70%), Query Frame = 1


Query: 14  ESVPESYVWPQEKRPGIALKK----TIPVLDFATQDRALLFQKILDSTHEFGVFQVINHG 73
           E++P  ++  ++++P I   +     IPV+D +  D   + + ++ ++ E+G+FQV+NHG
Sbjct: 17  EAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEESVRRAVVKASEEWGLFQVVNHG 76

Query: 74  VSKKLVEETMSVFKEFHSMPPQGKRREVS-KDNKSCKVFTSSNNYENEQTHLWRDCLSLT 133
           +  +L+     V ++F  +P   K      +D+K  + + +    + E    W D L   
Sbjct: 77  IPTELIRRLQDVGRKFFELPSSEKESVAKPEDSKDIEGYGTKLQKDPEGKKAWVDHLFHR 136

Query: 134 CYPDLHQNIHSWPQNPPKLREVVGAYCIAMEKFSREIIDLISEGLGLGQGYFE----GEM 193
            +P    N   WP+NPP+ REV   Y + ++K S  ++ ++S+GLGL +   +    GEM
Sbjct: 137 IWPPSCVNYRFWPKNPPEYREVNEEYAVHVKKLSETLLGILSDGLGLKRDALKEGLGGEM 196

Query: 194 SSYRRLTANHYPICPNPSLTMGLNQHCDRDLMTILF-QDVSGLQVFKDGHWISIDPIDDA 253
           + Y  +  N+YP CP P L +G+  H D   +T+L   +V GLQVFKD HW   + I  A
Sbjct: 197 AEYM-MKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQVFKDDHWFDAEYIPSA 256

Query: 254 FVVNFGYLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCLINEANPP 313
            +V+ G  +  +SNG+ K V HR   + E +R S+   + P +E I+ P   L  + NPP
Sbjct: 257 VIVHIGDQILRLSNGRYKNVLHRTTVDKEKTRMSWPVFLEPPREKIVGPLPELTGDDNPP 316

Query: 314 HYRSFKVEDFN 315
            ++ F  +D++
Sbjct: 317 KFKPFAFKDYS 326

[Close]

BLAST of Cucsa.388580 vs. TAIR10
Match: AT1G55290.1 (AT1G55290.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 165.6 bits (418), Expect = 5.2e-41
Identity = 101/334 (30.24%), Postives = 174/334 (52.10%), Query Frame = 1


Query: 12  GVESVPESYVWPQEKRP-GIALKK----TIPVLDFATQDRALLFQKILDSTHEFGVFQVI 71
           G++ +P+ Y+ P E+R     +K+    +IPV+D +  D   + + + D+  E+G FQVI
Sbjct: 33  GIKVLPDQYIQPFEERLINFHVKEDSDESIPVIDISNLDEKSVSKAVCDAAEEWGFFQVI 92

Query: 72  NHGVSKKLVEETMSVFKEFHSMPPQGKRREVSKDNKSCKV-FTSSNNYENEQTHLWRDCL 131
           NHGVS +++E   +    F  +P + KR+   + + S  V F +S +   E+   W+D L
Sbjct: 93  NHGVSMEVLENMKTATHRFFGLPVEEKRKFSREKSLSTNVRFGTSFSPHAEKALEWKDYL 152

Query: 132 SLTCYPDLHQNIHSWPQNPPKLREVVGAYCIAMEKFSREIIDLISEGLGLGQ------GY 191
           SL    +   +   WP +    R     Y    +   ++++  + E L + +       +
Sbjct: 153 SLFFVSEAEAS-QLWPDS---CRSETLEYMNETKPLVKKLLRFLGENLNVKELDKTKESF 212

Query: 192 FEGEMSSYRRLTANHYPICPNPSLTMGLNQHCDRDLMTILFQD-VSGLQV--FKDGHWIS 251
           F G      R+  N+YPICPNP LT+G+ +H D   +TIL QD + GL V     G W+ 
Sbjct: 213 FMGST----RINLNYYPICPNPELTVGVGRHSDVSSLTILLQDEIGGLHVRSLTTGRWVH 272

Query: 252 IDPIDDAFVVNFGYLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCL 311
           + PI  + V+N G  ++++SNG+ K+VEHRV+ N   +R S    + P+ E +I P   +
Sbjct: 273 VPPISGSLVINIGDAMQIMSNGRYKSVEHRVLANGSYNRISVPIFVSPKPESVIGPLLEV 332

Query: 312 INEANPPHYRSFKVEDFNRHFFAVSNNNEETMKF 331
           I     P Y+     D+ +HFF  +++ ++T+ F
Sbjct: 333 IENGEKPVYKDILYTDYVKHFFRKAHDGKKTIDF 358

[Close]

BLAST of Cucsa.388580 vs. NCBI nr
Match: gi|700189512|gb|KGN44745.1| (hypothetical protein Csa_7G376360 [Cucumis sativus])

HSP 1 Score: 689.9 bits (1779), Expect = 2.2e-195
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 1


Query: 1   MAQMNFVSNWEGVESVPESYVWPQEKRPGIALKKTIPVLDFATQDRALLFQKILDSTHEF 60
           MAQMNFVSNWEGVESVPESYVWPQEKRPGIALKKTIPVLDFATQDRALLFQKILDSTHEF
Sbjct: 1   MAQMNFVSNWEGVESVPESYVWPQEKRPGIALKKTIPVLDFATQDRALLFQKILDSTHEF 60

Query: 61  GVFQVINHGVSKKLVEETMSVFKEFHSMPPQGKRREVSKDNKSCKVFTSSNNYENEQTHL 120
           GVFQVINHGVSKKLVEETMSVFKEFHSMPPQGKRREVSKDNKSCKVFTSSNNYENEQTHL
Sbjct: 61  GVFQVINHGVSKKLVEETMSVFKEFHSMPPQGKRREVSKDNKSCKVFTSSNNYENEQTHL 120

Query: 121 WRDCLSLTCYPDLHQNIHSWPQNPPKLREVVGAYCIAMEKFSREIIDLISEGLGLGQGYF 180
           WRDCLSLTCYPDLHQNIHSWPQNPPKLREVVGAYCIAMEKFSREIIDLISEGLGLGQGYF
Sbjct: 121 WRDCLSLTCYPDLHQNIHSWPQNPPKLREVVGAYCIAMEKFSREIIDLISEGLGLGQGYF 180

Query: 181 EGEMSSYRRLTANHYPICPNPSLTMGLNQHCDRDLMTILFQDVSGLQVFKDGHWISIDPI 240
           EGEMSSYRRLTANHYPICPNPSLTMGLNQHCDRDLMTILFQDVSGLQVFKDGHWISIDPI
Sbjct: 181 EGEMSSYRRLTANHYPICPNPSLTMGLNQHCDRDLMTILFQDVSGLQVFKDGHWISIDPI 240

Query: 241 DDAFVVNFGYLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCLINEA 300
           DDAFVVNFGYLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCLINEA
Sbjct: 241 DDAFVVNFGYLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCLINEA 300

Query: 301 NPPHYRSFKVEDFNRHFFAVSNNNEETMKFILA 334
           NPPHYRSFKVEDFNRHFFAVSNNNEETMKFILA
Sbjct: 301 NPPHYRSFKVEDFNRHFFAVSNNNEETMKFILA 333

[Close]

BLAST of Cucsa.388580 vs. NCBI nr
Match: gi|659100769|ref|XP_008451257.1| (PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis melo])

HSP 1 Score: 434.9 bits (1117), Expect = 1.3e-118
Identity = 204/333 (61.26%), Postives = 256/333 (76.88%), Query Frame = 1


Query: 1   MAQMNFVSNWEGVESVPESYVWPQEKRPGIALKKTIPVLDFATQDRALLFQKILDSTHEF 60
           M+Q  FVSNW+GVESVPE+YV+P EKR G+++KKTIPV+D+A  DR LL +K+ D T EF
Sbjct: 2   MSQTKFVSNWKGVESVPETYVYPPEKRFGVSVKKTIPVIDWAIDDRTLLLKKVHDVTQEF 61

Query: 61  GVFQVINHGVSKKLVEETMSVFKEFHSMPPQGKRREVSKD--NKSCKVFTSSNNYENEQT 120
           G  QVINHGV K LVEETM + KEFH+M P+ K RE SKD  +KSC+V++SS NYE EQ 
Sbjct: 62  GFVQVINHGVPKTLVEETMKILKEFHAMSPEDKERECSKDPDHKSCRVYSSSQNYETEQI 121

Query: 121 HLWRDCLSLTCYPDLHQNIHSWPQNPPKLREVVGAYCIAMEKFSREIIDLISEGLGLGQG 180
           HLW+D L LTC+P+L +N+  WPQNPPK REVV AY + +EKF+ EI++++SEGLGL +G
Sbjct: 122 HLWKDTLKLTCHPNLDENVQFWPQNPPKFREVVKAYLLKIEKFALEILEVLSEGLGLEKG 181

Query: 181 YFEGEMSSYRRLTANHYPICPNPSLTMGLNQHCDRDLMTILFQDVSGLQVFKDGHWISID 240
           Y  GEMS    L  +HYP CPNPSLT+GLNQH D  L+TILFQDV+GLQ  KDG WI +D
Sbjct: 182 YLRGEMSKNPELFVHHYPPCPNPSLTIGLNQHADPTLITILFQDVNGLQFLKDGQWIDVD 241

Query: 241 PIDDAFVVNFGYLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCLIN 300
           P+ +AF+VN GY+LEVISNGK+KAVEHRVVTN   SRQS  YV++P  ++ IEP+KC IN
Sbjct: 242 PLPNAFLVNLGYVLEVISNGKMKAVEHRVVTNTTTSRQSLAYVVYPGNDVNIEPSKCFIN 301

Query: 301 EANPPHYRSFKVEDFNRHFFAVSNNNEETMKFI 332
           EANPPHY S + +DF +   +   + E  +KFI
Sbjct: 302 EANPPHYLSLEFKDFIKKLMSKPPDRETALKFI 334

[Close]

BLAST of Cucsa.388580 vs. NCBI nr
Match: gi|449458500|ref|XP_004146985.1| (PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis sativus])

HSP 1 Score: 424.9 bits (1091), Expect = 1.3e-115
Identity = 200/330 (60.61%), Postives = 254/330 (76.97%), Query Frame = 1


Query: 6   FVSNWEGVESVPESYVWPQEKRPG---IALKKTIPVLDFATQDRALLFQKILDSTHEFGV 65
           FVSNWE VESVPESYV+P EKRPG   + + K IPV+D + +DR LL +KILD++ EFG 
Sbjct: 8   FVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEFGF 67

Query: 66  FQVINHGVSKKLVEETMSVFKEFHSMPPQGKRREVSKD-NKSCKVFTSSNNYENEQTHLW 125
           FQ+INHGVSKK+ EETM +FKEFH+M    K +E SKD N+SC+V+TSS NY  EQ H W
Sbjct: 68  FQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQVHCW 127

Query: 126 RDCLSLTCYPDLHQNIHSWPQNPPKLREVVGAYCIAMEKFSREIIDLISEGLGLGQGYFE 185
           RD L   C+P L + +H WPQ PPK REVVGAYC+AM K   EI++L+SEGLGLG+GYF 
Sbjct: 128 RDALIFNCHP-LEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYFG 187

Query: 186 GEMSSYRRLTANHYPICPNPSLTMGLNQHCDRDLMTILFQDVSGLQVFKDGHWISIDPID 245
           GEMS    L  NHYP CPNPSLT+GL+QHCD  L+TILFQDV+GLQV KDG WI + PID
Sbjct: 188 GEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQPID 247

Query: 246 DAFVVNFGYLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCLINEAN 305
           +AFVVN G++L++I+NGKL+A EHR VTN++ SRQS  Y+++P+ E ++EPAKC+INEAN
Sbjct: 248 NAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEAN 307

Query: 306 PPHYRSFKVEDFNRHFFAVSNNNEETMKFI 332
           PP YRS   +DF R++   + + +  M++I
Sbjct: 308 PPRYRSLNFKDFQRNYLPRAVDTKAVMQYI 336

[Close]

BLAST of Cucsa.388580 vs. NCBI nr
Match: gi|449462457|ref|XP_004148957.1| (PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis sativus])

HSP 1 Score: 422.9 bits (1086), Expect = 5.1e-115
Identity = 201/332 (60.54%), Postives = 253/332 (76.20%), Query Frame = 1


Query: 1   MAQMNFVSNWEGVESVPESYVWPQEKRPGIALKKTIPVLDFATQDRALLFQKILDSTHEF 60
           M++  FVSNW GV SVPE+YV+P EKR GI++KK+IPV+D++  DRALL +++ D T EF
Sbjct: 2   MSRTKFVSNWGGVVSVPETYVYPPEKRFGISVKKSIPVIDWS-DDRALLLKRVHDVTQEF 61

Query: 61  GVFQVINHGVSKKLVEETMSVFKEFHSMPPQGKRREVSKD-NKSCKVFTSSNNYENEQTH 120
           G  QVINHGV K LVEE M + KEFH M  + K RE SKD +KSCKV+TSS NYENEQ H
Sbjct: 62  GFVQVINHGVPKTLVEEMMKIMKEFHGMSAEDKERECSKDPDKSCKVYTSSQNYENEQIH 121

Query: 121 LWRDCLSLTCYPDLHQNIHSWPQNPPKLREVVGAYCIAMEKFSREIIDLISEGLGLGQGY 180
           LW+D   L C+P+L +N+  WPQNPPKLREVV AY + +EKF+ EI++++SEGLGL +GY
Sbjct: 122 LWKDTFKLRCHPNLDKNLEFWPQNPPKLREVVKAYIVMIEKFASEILEVLSEGLGLEKGY 181

Query: 181 FEGEMSSYRRLTANHYPICPNPSLTMGLNQHCDRDLMTILFQDVSGLQVFKDGHWISIDP 240
           F GEMS      A+HYP CPNPSLT+GL+QH D  L+TILFQDV+GLQ  KDG WI +DP
Sbjct: 182 FRGEMSKNPEFLAHHYPPCPNPSLTVGLSQHADPTLITILFQDVNGLQFLKDGQWIDVDP 241

Query: 241 IDDAFVVNFGYLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCLINE 300
           + DAF+VN GY+ EVISNGKLKA EHRVVTN+  SRQ+  YV++PE ++ IEP+KC INE
Sbjct: 242 LPDAFLVNLGYVFEVISNGKLKAAEHRVVTNSTTSRQTLVYVVYPENDVTIEPSKCFINE 301

Query: 301 ANPPHYRSFKVEDFNRHFFAVSNNNEETMKFI 332
           ANPPHYRS + +DF     +   + +  M+FI
Sbjct: 302 ANPPHYRSLEFKDFYNKLMSKPPDRKTAMEFI 332

[Close]

BLAST of Cucsa.388580 vs. NCBI nr
Match: gi|700189511|gb|KGN44744.1| (hypothetical protein Csa_7G375860 [Cucumis sativus])

HSP 1 Score: 420.2 bits (1079), Expect = 3.3e-114
Identity = 197/332 (59.34%), Postives = 250/332 (75.30%), Query Frame = 1


Query: 1   MAQMNFVSNWEGVESVPESYVWPQEKRPGIALKKTIPVLDFATQDRALLFQKILDSTHEF 60
           M+Q  FVSNW+G+ SVPE+YV P EKR G+++KK IPV+D AT+DR LL +K+LD + EF
Sbjct: 1   MSQTKFVSNWKGIGSVPETYVCPPEKRVGVSVKKKIPVIDLATEDRDLLSKKVLDVSQEF 60

Query: 61  GVFQVINHGVSKKLVEETMSVFKEFHSMPPQGKRREVSKD-NKSCKVFTSSNNYENEQTH 120
           G FQVINHGVSK LVEETM V +EFH+M  + K  E SKD NKSC +++S+ NYE E+ H
Sbjct: 61  GFFQVINHGVSKALVEETMRVSREFHAMSSEDKEMECSKDPNKSCLIYSSTPNYEKEELH 120

Query: 121 LWRDCLSLTCYPDLHQNIHSWPQNPPKLREVVGAYCIAMEKFSREIIDLISEGLGLGQGY 180
           LW+D L L CYP+L++N+  WPQNPPK REV+ AY + M+KFS EI++++SEGLGL + Y
Sbjct: 121 LWKDSLRLICYPNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLEKEY 180

Query: 181 FEGEMSSYRRLTANHYPICPNPSLTMGLNQHCDRDLMTILFQDVSGLQVFKDGHWISIDP 240
           F G MS+      +HYP CPNPSLT+GLN+H D  ++TILFQDV+GLQ  KDG WI +DP
Sbjct: 181 FSGGMSNNPEFLVHHYPPCPNPSLTLGLNKHADPTVITILFQDVNGLQFLKDGEWIDVDP 240

Query: 241 IDDAFVVNFGYLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCLINE 300
           + DAF+VN GY+ EVISNGK+KA EHRVVTN   SRQS  Y ++PE  M IEP+KC INE
Sbjct: 241 LPDAFLVNLGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCFINE 300

Query: 301 ANPPHYRSFKVEDFNRHFFAVSNNNEETMKFI 332
           ANPPHYR+ + +DF     +   + E  MKFI
Sbjct: 301 ANPPHYRTLEFKDFVNKLRSKHPDREMAMKFI 332

The following BLAST results are available for this feature:

BLAST of Cucsa.388580 vs. Swiss-Prot


Match Name	E-value	Identity	Description
HY6H_HYONI	3.2e-61	39.56	Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger GN=H6H PE=1 SV=1	[more]
DMR6_ARATH	2.4e-56	36.70	Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana GN=DMR6 PE=1 SV=1	[more]
IDS3_HORVU	4.1e-56	37.54	2'-deoxymugineic-acid 2'-dioxygenase OS=Hordeum vulgare GN=IDS3 PE=1 SV=3	[more]
IDS2_HORVU	3.8e-54	38.61	Mugineic-acid 3-dioxygenase OS=Hordeum vulgare GN=IDS2 PE=2 SV=1	[more]
DLO2_ARATH	2.8e-49	34.88	Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana GN=DLO2 PE=2 SV=1	[more]

BLAST of Cucsa.388580 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K4U9_CUCSA	1.6e-195	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G376360 PE=3 SV=1	[more]
A0A0A0K5H3_CUCSA	9.3e-116	60.61	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G378370 PE=3 SV=1	[more]
A0A0A0K5H0_CUCSA	3.5e-115	60.54	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G375850 PE=3 SV=1	[more]
A0A0A0K745_CUCSA	2.3e-114	59.34	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G375860 PE=3 SV=1	[more]
A0A061DWT9_THECC	3.8e-85	47.76	2-oxoglutarate and Fe(II)-dependent oxygenase superfamily protein, putative OS=T...	[more]

BLAST of Cucsa.388580 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G24530.1	1.4e-57	36.70	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT4G10490.1	1.6e-50	34.88	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT4G10500.1	4.8e-47	34.77	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT5G08640.1	2.9e-44	30.87	flavonol synthase 1	[more]
AT1G55290.1	5.2e-41	30.24	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]

BLAST of Cucsa.388580 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700189512\|gb\|KGN44745.1\|	2.2e-195	100.00	hypothetical protein Csa_7G376360 [Cucumis sativus]	[more]
gi\|659100769\|ref\|XP_008451257.1\|	1.3e-118	61.26	PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis melo]	[more]
gi\|449458500\|ref\|XP_004146985.1\|	1.3e-115	60.61	PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis sativus]	[more]
gi\|449462457\|ref\|XP_004148957.1\|	5.1e-115	60.54	PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis sativus]	[more]
gi\|700189511\|gb\|KGN44744.1\|	3.3e-114	59.34	hypothetical protein Csa_7G375860 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR005123	Oxoglu/Fe-dep_dioxygenase
IPR026992	DIOX_N
IPR027443	IPNS-like

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016706	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.388580.1	Cucsa.388580.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PFAM	PF03171	2OG-FeII_Oxy	coord: 192..284 score: 6.5
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROFILE	PS51471	FE2OG_OXY	coord: 185..286 score: 11
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 36..142 score: 3.4
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 12..326 score: 2.6
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 1..327 score: 8.9E
None	No IPR available	PANTHER	PTHR10209:SF124	SUBFAMILY NOT NAMED	coord: 1..327 score: 8.9E
None	No IPR available	unknown	SSF51197	Clavaminate synthase-like	coord: 11..319 score: 3.23

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.388580	Cp4.1LG17g06100	Cucurbita pepo (Zucchini)	cgycpeB1032

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.388580	Cucurbita maxima (Rimu)	cgycmaB1079
Cucsa.388580	Cucurbita moschata (Rifu)	cgycmoB1078
Cucsa.388580	Melon (DHL92) v3.5.1	cgymeB636
Cucsa.388580	Wild cucumber (PI 183967)	cgycpiB574
Cucsa.388580	Cucumber (Chinese Long) v2	cgycuB545
Cucsa.388580	Melon (DHL92) v3.5.1	cgymeB640
Cucsa.388580	Watermelon (Charleston Gray)	cgywcgB667
Cucsa.388580	Watermelon (97103) v1	cgywmB702
Cucsa.388580	Bottle gourd (USVL1VR-Ls)	cgylsiB631
Cucsa.388580	Melon (DHL92) v3.6.1	cgymedB631
Cucsa.388580	Melon (DHL92) v3.6.1	cgymedB634
Cucsa.388580	Cucumber (Chinese Long) v3	cgycucB590
Cucsa.388580	Watermelon (97103) v2	cgywmbB670
Cucsa.388580	Wax gourd	cgywgoB777
Cucsa.388580	Wax gourd	cgywgoB778