Cucsa.374170 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.374170 vs. Swiss-Prot
Match: PME45_ARATH (Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana GN=PME45 PE=2 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 1.5e-124
Identity = 237/529 (44.80%), Postives = 337/529 (63.71%), Query Frame = 1

Query: 2   KAVSTICSTANYQEECQNTLTNAAH-NASSDDPKEYVKAAILATIDEVKKGYNLTDGFLI 61
           K + T+C++  Y+  CQNTL N    +    DP+  +K+AI+A  D++ + +        
Sbjct: 93  KIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQVFKRVLSLKT 152

Query: 62  EAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQ 121
           E   N+  K  +  C+ L+  A ++L  S   + + +++  A  V D+ +WL++V+SYQ+
Sbjct: 153 E---NKDDKDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDSWLSAVMSYQE 212

Query: 122 SCLDGLEEFDPQLRQKMQDGLNGAGKLTSNALAIVDAVSDILASFG-----LQLKAQPSG 181
           +C+DG EE   +L+ +++   N +  LTSN+LA++ ++   L+S       L L+A+ S 
Sbjct: 213 TCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVPKVKTRLLLEARSSA 272

Query: 182 RRLLGTTEVDNDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYP 241
           +        + D   +WL+  +R++L +    A +KPNA VAKDGSG F TI+AAL A P
Sbjct: 273 K--------ETDHITSWLSNKERRMLKAVDVKA-LKPNATVAKDGSGNFTTINAALKAMP 332

Query: 242 KTLRGRYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFI 301
              +GRY IY+K GIY E V I K   N+ M GDG +KTIVTGNKS+     T  TATF+
Sbjct: 333 AKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKKIRTFLTATFV 392

Query: 302 AIGEGFLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNC 361
           A GEGF+ +SMGF+NTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY  T RQ+YR+C
Sbjct: 393 AQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYAYTHRQYYRSC 452

Query: 362 VISGTVDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQ 421
           VI GTVDFIFGD+  + QN  I +R+ +  Q+NTVTA GR D  + +G VIHNC + P +
Sbjct: 453 VIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFVIHNCTVAPNE 512

Query: 422 KLFAERFKIPTFLGRPWKEYARTVIMESTMGDFIQPVGYMPW-SGNFALETCLYLEYGNR 481
            L   + +  ++LGRPWK ++RTV+MEST+ D I PVG++ W   +FA++T  Y EY N 
Sbjct: 513 DLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAIDTLSYAEYKND 572

Query: 482 GPGAVTNRRVRWKGVKVIGRNEAMQFTAGSFLQGKTWLPTTGGPYLLGL 524
           GP   T  RV+W G +V+ + EAM+FT G FLQG+ W+   G P  LGL
Sbjct: 573 GPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE-WIQAIGSPVKLGL 606

BLAST of Cucsa.374170 vs. Swiss-Prot
Match: PME21_ARATH (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana GN=PME21 PE=2 SV=2)

HSP 1 Score: 442.2 bits (1136), Expect = 8.0e-123
Identity = 214/523 (40.92%), Postives = 326/523 (62.33%), Query Frame = 1

Query: 1   MKAVSTICSTANYQEECQNTLTNAAHNASSDDPKEYVKAAILATIDEVKKGYNLTDGFLI 60
           +KAV  +C+  +Y++ C++TL     N +  DP E VK A   T+ ++      +   ++
Sbjct: 55  VKAVKDVCAPTDYRKTCEDTLIKNGKNTT--DPMELVKTAFNVTMKQITDAAKKSQT-IM 114

Query: 61  EAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQ 120
           E   +   +M ++ C++L+ +A+D+L  S+  +G+ + H   + + +++ WL++ IS+++
Sbjct: 115 ELQKDSRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEE 174

Query: 121 SCLDGLEEFDPQLRQKMQDGLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLG 180
           +CL+G +       + M+  L  A +LT N LAI+  +S+ +    +Q+    S RRLL 
Sbjct: 175 TCLEGFQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQ--MQIPGLNS-RRLLA 234

Query: 181 TTEVDNDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRG 240
                 +G+P+W+    RKLL +    + VKP+ VVA+DGSGQ+KTI+ AL   PK    
Sbjct: 235 ------EGFPSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNT 294

Query: 241 RYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEG 300
            +V+++KAG+YKEYV + K M ++   GDGP KTI++GNK+ + G TT  TAT   +G  
Sbjct: 295 TFVVHIKAGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNY 354

Query: 301 FLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGT 360
           F+ K++GF+NTAG   HQAVA+RVQSD S FFNCR DGYQDTLY  + RQF+R+C ISGT
Sbjct: 355 FIAKNIGFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGT 414

Query: 361 VDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAE 420
           +DF+FGD+  V QN  ++VR+P+ NQ   +TA+GR DP+E +G V   C I  E    A 
Sbjct: 415 IDFLFGDAAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAV 474

Query: 421 RFKIPTFLGRPWKEYARTVIMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVT 480
           +     +LGRPWKEY+RT+IM + + DF+QP G+ PW G+F L+T  Y E  N GPG+  
Sbjct: 475 KETSKAYLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSAL 534

Query: 481 NRRVRWKGVKVIGRNEAMQFTAGSFLQGKTWLPTTGGPYLLGL 524
             RV W G+K +   + ++FT   ++QG  W+P  G PY  GL
Sbjct: 535 ANRVTWAGIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGL 565

BLAST of Cucsa.374170 vs. Swiss-Prot
Match: PME13_ARATH (Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana GN=PME13 PE=2 SV=2)

HSP 1 Score: 439.1 bits (1128), Expect = 6.8e-122
Identity = 231/524 (44.08%), Postives = 336/524 (64.12%), Query Frame = 1

Query: 2   KAVSTICSTANYQEECQNTLTNAAHNASS-DDPKEYVKAAILATIDEVKKGYNLTDGFLI 61
           K + T+CS+  Y + C+ TL N      + D+P  ++K+AI A  +++     + +  L 
Sbjct: 107 KIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDL---VLEKVLS 166

Query: 62  EAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQ 121
               N+  K  +E C+ L++ A ++  AS + +   ++++    V D+++WL++V+SYQ+
Sbjct: 167 LKTENQDDKDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQE 226

Query: 122 SCLDGLEEFDPQLRQKMQDGLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLG 181
           +CLDG EE +  L+ +++  +N +  LTSN+LA++   ++ L+     ++     R LL 
Sbjct: 227 TCLDGFEEGN--LKSEVKTSVNSSQVLTSNSLALIKTFTENLSPVMKVVE-----RHLL- 286

Query: 182 TTEVDNDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRG 241
                 D  P+W++  DR++L +    A +KPNA VAKDGSG F TI+ AL A P+   G
Sbjct: 287 ------DDIPSWVSNDDRRMLRAVDVKA-LKPNATVAKDGSGDFTTINDALRAMPEKYEG 346

Query: 242 RYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEG 301
           RY+IYVK GIY EYV + K   N+ M GDG +KTIVTGNKS+     T  TATF+A GEG
Sbjct: 347 RYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEG 406

Query: 302 FLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGT 361
           F+ +SMGF+NTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY  T RQ+YR+CVI GT
Sbjct: 407 FMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGT 466

Query: 362 VDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAE 421
           +DFIFGD+  + QN  I +R+ +  Q+NTVTA GR D  + +G V+HNC+I   + L   
Sbjct: 467 IDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPV 526

Query: 422 RFKIPTFLGRPWKEYARTVIMESTMGDFIQPVGYMPW-SGNFALETCLYLEYGNRGPGAV 481
           + +  ++LGRPWK Y+RT+IMES + + I PVG++ W   +FA++T  Y EY N+G    
Sbjct: 527 KEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGD 586

Query: 482 TNRRVRWKGVKVIGRNEAMQFTAGSFLQGKTWLPTTGGPYLLGL 524
           T  RV+W G KVI + EA+ +T G FLQG  W+  +G P  LGL
Sbjct: 587 TTSRVKWPGFKVINKEEALNYTVGPFLQG-DWISASGSPVKLGL 611

BLAST of Cucsa.374170 vs. Swiss-Prot
Match: PME28_ARATH (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana GN=PME28 PE=2 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 4.4e-121
Identity = 215/525 (40.95%), Postives = 327/525 (62.29%), Query Frame = 1

Query: 1   MKAVSTICSTANYQEECQNTLTNAAHNASSDDPKEYVKAAILATIDEVKKGYNLTDGFLI 60
           +KA+  +C+  +Y+E C++TL   A + S  DP E VK A  AT+ ++      +   +I
Sbjct: 54  VKAIKDVCAPTDYKETCEDTLRKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQT-MI 113

Query: 61  EAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQ 120
           E   +   KM ++ C++L+ +AI +L  S+  +G+ + H   + +  ++ WL++ IS++Q
Sbjct: 114 ELQKDPRAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQ 173

Query: 121 SCLDGLEEFDPQLRQKMQDGLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLG 180
           +CLDG +       + ++  L  A +LT N LA+V  +S+ L    +    + + RRLL 
Sbjct: 174 TCLDGFQGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIP---EMNSRRLL- 233

Query: 181 TTEVDNDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRG 240
                +  +P+W+    R+LL      ++VKP+ VVA+DGSGQ+KTI+ AL   PK    
Sbjct: 234 -----SQEFPSWMDARARRLL--NAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNT 293

Query: 241 RYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEG 300
            +V+++K GIYKEYV + + M ++   GDGP KT+++G+KS + G TT  TAT   +G+ 
Sbjct: 294 TFVVHIKEGIYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDH 353

Query: 301 FLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGT 360
           F+ K++ F+NTAG   HQAVA+RV +D S F+NC+ DGYQDTLY  + RQFYR+C ISGT
Sbjct: 354 FIAKNIAFENTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGT 413

Query: 361 VDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAE 420
           +DF+FGD+  V QN  ++VR+P+ NQ   +TA+GR DP+E +G V+  C IV E    A 
Sbjct: 414 IDFLFGDAAAVFQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAV 473

Query: 421 RFKIPTFLGRPWKEYARTVIMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVT 480
           + +  T+LGRPWKEY+RT+IM + + DF+ P G+ PW G F L T  Y E  N GPGA  
Sbjct: 474 KEQSKTYLGRPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAI 533

Query: 481 NRRVRWKGVKVIGRNEAMQFTAGSFLQGKTWLPTTGGPYLLGLKS 526
            +RV W G+K +   E ++FT   ++QG  W+P  G PY+LGL S
Sbjct: 534 TKRVTWPGIKKLSDEEILKFTPAQYIQGDAWIPGKGVPYILGLFS 564

BLAST of Cucsa.374170 vs. Swiss-Prot
Match: PME4_ARATH (Pectinesterase 4 OS=Arabidopsis thaliana GN=PME4 PE=2 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 1.1e-119
Identity = 247/564 (43.79%), Postives = 333/564 (59.04%), Query Frame = 1

Query: 1   MKAVSTICSTANYQEECQNTLTNAAHNASSDDPKEYVKAAILATIDEVKKGYNLTDGFLI 60
           MKAV  IC + + +  C  TL        S+DP + +KA +LAT DE+ K  N T    +
Sbjct: 38  MKAVQGICQSTSDKASCVKTL----EPVKSEDPNKLIKAFMLATKDELTKSSNFTGQTEV 97

Query: 61  E-----AANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSV 120
                 + NN+++   ++ C+ +  +A++ L      +GE DL     ++  +K WL  V
Sbjct: 98  NMGSSISPNNKAV---LDYCKRVFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGV 157

Query: 121 ISYQQSCLDGLEEFDPQLRQKMQDGLNGAGKLTSNALAIVDAVSDILASFGLQLK----- 180
            +YQ  CLD +EE D  LR+ + +G+  +  LT+NA+ I   V   +A    ++      
Sbjct: 158 YNYQTDCLDDIEEDD--LRKAIGEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNM 217

Query: 181 ------------------AQPSG--RRLLGTTEVDNDGYPTWLTGADRKLLA-------- 240
                             A P G  RRLL   ++D  G PTW++GADRKL+A        
Sbjct: 218 TGGIPTPGAPPVVDESPVADPDGPARRLL--EDIDETGIPTWVSGADRKLMAKAGRGRRG 277

Query: 241 SRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRGRYVIYVKAGIYKEYVHITKDMK 300
            RGGGA+V+ N VVAKDGSGQFKT+  A+ A P+  RGR +IY+KAG+Y+E V I K   
Sbjct: 278 GRGGGARVRTNFVVAKDGSGQFKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKN 337

Query: 301 NIFMYGDGPKKTIVTGNKS--NRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAV 360
           NIFM+GDG +KT+++ N+S     G TT  +AT     EGF+ K MGF+NTAGP GHQA 
Sbjct: 338 NIFMFGDGARKTVISYNRSVALSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAA 397

Query: 361 ALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIVR 420
           A+RV  DR+  FNCR DGYQDTLYV   RQFYRNCV+SGTVDFIFG S TVIQN+LI+VR
Sbjct: 398 AIRVNGDRAVIFNCRFDGYQDTLYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVR 457

Query: 421 RPMDNQQNTVTANGRADPKEVS-GLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480
           +    Q NTVTA+G      +  G+V+ NCRIVP++KL  ER  + T+LGRPWK+++ TV
Sbjct: 458 KGSKGQYNTVTADGNELGLGMKIGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTV 517

Query: 481 IMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQ 524
           IM + MGD I+P G+  W G    ++C Y+EY NRGPGA  NRRV W  V      E   
Sbjct: 518 IMSTEMGDLIRPEGWKIWDGESFHKSCRYVEYNNRGPGAFANRRVNWAKV-ARSAAEVNG 577

BLAST of Cucsa.374170 vs. TrEMBL
Match: A0A0A0KLN2_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_5G119700 PE=4 SV=1)

HSP 1 Score: 905.2 bits (2338), Expect = 3.7e-260
Identity = 436/525 (83.05%), Postives = 478/525 (91.05%), Query Frame = 1

Query: 1   MKAVSTICSTANYQEECQNTLTNAAHNASSDDPKEYVKAAILATIDEVKKGYNLTDGFLI 60
           MKAV++ICS A+YQ ECQ TL +  +N+S +DPKE++KAAI +TI+E+KKGYNLTD  ++
Sbjct: 42  MKAVASICSNADYQPECQTTLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMV 101

Query: 61  EAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQ 120
           EAANN +IKM V+DC+DLLQ AID+L ASYSTVG+PDLHTN DR+ADIKNWLT+VISYQQ
Sbjct: 102 EAANNATIKMSVDDCKDLLQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQ 161

Query: 121 SCLDGLEEFDPQLRQKMQDGLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLG 180
           SCLDGLEEFDPQL+QKMQDGL+ AGKLTSNALAIV AVSDIL   GLQLK QPSGRRLLG
Sbjct: 162 SCLDGLEEFDPQLKQKMQDGLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLG 221

Query: 181 TTEVDNDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRG 240
           TTEVD+D +PTWLTG+DRKLLA++ GG +VKPN VVAKDGSGQ+KTI AALAAYPK L+G
Sbjct: 222 TTEVDSDSFPTWLTGSDRKLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKG 281

Query: 241 RYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEG 300
           RYVIYVKAG+Y E + +TKDMKNIFMYGDGP+KTIVTG KSNR G TTQ+TA+F AIGEG
Sbjct: 282 RYVIYVKAGVYNEPIIVTKDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEG 341

Query: 301 FLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGT 360
           FLCKSMGF NTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQT RQFYRNCVISGT
Sbjct: 342 FLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGT 401

Query: 361 VDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAE 420
           VDFIFGDS T+IQNSLIIVRRPMDNQQNTVTANGRAD KE+SGLVIHNCRIVPEQKLFAE
Sbjct: 402 VDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAE 461

Query: 421 RFKIPTFLGRPWKEYARTVIMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVT 480
           RFKIPTFLGRPWKEYARTVIMEST+GDFIQP GYMPWSGNFAL TC Y EYGNRGPGA T
Sbjct: 462 RFKIPTFLGRPWKEYARTVIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANT 521

Query: 481 NRRVRWKGVKVIGRNEAMQFTAGSFLQGKTWLPTTGGPYLLGLKS 526
           NRRVRWKGV+VIGRNEAMQFTAG FL GK WLP TGGPYLLGLK+
Sbjct: 522 NRRVRWKGVRVIGRNEAMQFTAGPFLLGKAWLPGTGGPYLLGLKN 566

BLAST of Cucsa.374170 vs. TrEMBL
Match: A0A0A0KJ73_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_5G125700 PE=4 SV=1)

HSP 1 Score: 887.5 bits (2292), Expect = 8.0e-255
Identity = 428/525 (81.52%), Postives = 479/525 (91.24%), Query Frame = 1

Query: 1   MKAVSTICSTANYQEECQNTLTNAAHNASSDDPKEYVKAAILATIDEVKKGYNLTDGFLI 60
           MKAVS ICST +YQ+EC+ TL + A N SS+DPK+Y +AAILATI E+ KGYNL+D  ++
Sbjct: 43  MKAVSAICSTTDYQDECKTTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIV 102

Query: 61  EAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQ 120
           EA+ N SIKM VEDC+DLLQFAID+LQASYS VGE DLHT++DRVADIKNWL++VISYQQ
Sbjct: 103 EASTNASIKMSVEDCKDLLQFAIDELQASYSAVGESDLHTDSDRVADIKNWLSAVISYQQ 162

Query: 121 SCLDGLEEFDPQLRQKMQDGLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLG 180
           SCLDGL EFDPQL+Q+MQDGL+ AGKLTSNALAIV AVS+IL ++ LQLK QPSGRRLLG
Sbjct: 163 SCLDGLGEFDPQLKQRMQDGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLG 222

Query: 181 TTEVDNDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRG 240
           TT VD DG+PTWLTGADRKLLAS+  G +  PNAVVAKDGSG++KTI+AALAAYPK LRG
Sbjct: 223 TTVVDRDGFPTWLTGADRKLLASKQRGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRG 282

Query: 241 RYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEG 300
           RYVIYVKAGIY EY+ +TKDMKN+FMYGDGP+KTIVTG KSNR GFTTQ+TA+F AIGEG
Sbjct: 283 RYVIYVKAGIYDEYITLTKDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEG 342

Query: 301 FLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGT 360
           FLCKSMGF NTAGPEGHQAVALRVQSDRSAFFNCRMDG+QDTLYVQT RQFYRNCV+SGT
Sbjct: 343 FLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGT 402

Query: 361 VDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAE 420
           VDFIFGDS+TVIQNSLIIVRRPMDNQQNTVTA GRA+ KE++GLVIHNCRIVPEQKLFAE
Sbjct: 403 VDFIFGDSSTVIQNSLIIVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAE 462

Query: 421 RFKIPTFLGRPWKEYARTVIMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVT 480
           RFKIPTFLGRPWK+YARTVIMEST+GDFIQP GYMPWSG+FALETCLYLEYGNRGPGA T
Sbjct: 463 RFKIPTFLGRPWKQYARTVIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANT 522

Query: 481 NRRVRWKGVKVIGRNEAMQFTAGSFLQGKTWLPTTGGPYLLGLKS 526
           NRRVRWKG KVIGRNEA+Q+TAG+FL G++WLPTTGG Y LGLK+
Sbjct: 523 NRRVRWKGAKVIGRNEALQYTAGAFLLGRSWLPTTGGLYYLGLKN 567

BLAST of Cucsa.374170 vs. TrEMBL
Match: W9QN86_9ROSA (Pectinesterase OS=Morus notabilis GN=L484_013873 PE=4 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 3.8e-196
Identity = 335/524 (63.93%), Postives = 416/524 (79.39%), Query Frame = 1

Query: 4   VSTICSTANYQEECQNTLTNAAHNASSDDPKEYVKAAILATIDEVKKGYNLTDGFLIEAA 63
           V ++CS  +Y+  C+ TL+  +HN ++D  K+ +KAA+ AT+D+V K  NL++  L+EA 
Sbjct: 50  VESVCSNTDYKTACEQTLSPLSHNVTADY-KDIIKAAVQATVDQVSKSLNLSESLLVEAN 109

Query: 64  NNRS--IKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQS 123
           NN S  +KM +EDC DLL  AI QLQAS+S VG+ D+HT  DR A++++WL+SVISY ++
Sbjct: 110 NNNSTRLKMALEDCNDLLSLAIGQLQASFSMVGDSDMHTIKDRSAELRSWLSSVISYAET 169

Query: 124 CLDGLEEFDPQLRQKMQDGLNGAGKLTSNALAIVDAVSDILASFGLQLKAQP-SGRRLLG 183
           C+DG+EE  P+ + +++  L  A  LT NALAIV  +S+IL++FGL+L  +  +GRRLL 
Sbjct: 170 CIDGVEE--PKFQGQLKGTLQNATALTDNALAIVSTISEILSAFGLKLNPKALNGRRLLS 229

Query: 184 TTEVDNDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRG 243
            T + NDG PTW + +DRKL+A  G G ++KP+AVVAKDGSGQFKTI+AALAAYPK   G
Sbjct: 230 DTVIGNDGLPTWFSASDRKLMALHGNG-RIKPHAVVAKDGSGQFKTIAAALAAYPKNHVG 289

Query: 244 RYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEG 303
           RY+IYVKAG+Y EY+ + K+M NIFMYGDGP+KTIVTG KS  GGF+T  TA+F  IG G
Sbjct: 290 RYIIYVKAGVYNEYITVEKNMVNIFMYGDGPRKTIVTGRKSYTGGFSTFRTASFSVIGAG 349

Query: 304 FLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGT 363
           F+CK MGFQNTAGPEGHQAVALRVQS+ SAFFNCRMDGYQDTLYVQ  RQFYRNCVISGT
Sbjct: 350 FVCKGMGFQNTAGPEGHQAVALRVQSEMSAFFNCRMDGYQDTLYVQAGRQFYRNCVISGT 409

Query: 364 VDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAE 423
           +DFIFGD+T VIQNSL+IVRRPMDNQ N VTA GR +  + +G+VIHNCRIVPEQKLFA+
Sbjct: 410 IDFIFGDATVVIQNSLLIVRRPMDNQLNAVTAQGRKERHQPTGIVIHNCRIVPEQKLFAD 469

Query: 424 RFKIPTFLGRPWKEYARTVIMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVT 483
           RFKIPT+LGRPWKE++RTVIMEST+ DFIQP G+ PW+GNFAL+T  Y EY NRGPGA T
Sbjct: 470 RFKIPTYLGRPWKEFSRTVIMESTLADFIQPAGWFPWAGNFALDTLYYAEYANRGPGART 529

Query: 484 NRRVRWKGVKVIGRNEAMQFTAGSFLQGKTWLPTTGGPYLLGLK 525
           NRRV W+G  VIGR+EA QFT G FL+G+ WLP TG P+L   K
Sbjct: 530 NRRVHWRGYHVIGRHEAQQFTVGQFLEGQRWLPATGAPFLPSFK 569

BLAST of Cucsa.374170 vs. TrEMBL
Match: F6HZG5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_07s0005g02750 PE=4 SV=1)

HSP 1 Score: 668.3 bits (1723), Expect = 7.6e-189
Identity = 330/526 (62.74%), Postives = 406/526 (77.19%), Query Frame = 1

Query: 1   MKAVSTICSTANYQEECQNTLTNAAHNASSDDPKEYVKAAILATIDEVKKGYNLTDGFLI 60
           MKAV+++C+TA+Y++ C  TL+    N SS  PK+Y++AA+  TI ++K   NL++  L 
Sbjct: 292 MKAVASVCATADYKDACMQTLSPVPKNGSSATPKDYIQAAVQVTIKQIKSSMNLSEK-LF 351

Query: 61  EAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQ 120
           +A N+   +M + DC+DLLQFAID+LQ S+S+VGE DL T      +I NWL++ +SYQQ
Sbjct: 352 QATNDSRTQMALGDCKDLLQFAIDELQESFSSVGESDLQTLDQLSTEIMNWLSAAVSYQQ 411

Query: 121 SCLDGLEEFDPQLRQKMQDGLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLG 180
           +CLDG+ E  P+ +  MQ GL  A +LTSNALAIV  +S IL  F + L  +P+ RRLLG
Sbjct: 412 TCLDGVIE--PRFQAAMQKGLLNATQLTSNALAIVSDLSQILTKFNVPLDLKPNSRRLLG 471

Query: 181 TTEV-DNDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLR 240
             EV  +DGYPTW +  DRKLLA +  G ++ PNA+VAKDGSG F TI+AALAAYPK L+
Sbjct: 472 EIEVLGHDGYPTWFSATDRKLLALQDNG-RLTPNAIVAKDGSGHFTTIAAALAAYPKNLK 531

Query: 241 GRYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGE 300
           GRYVIYVKAGIY+EY+ +TKD  N++MYGDGP+KTIVTG K  R G TT  TATF AIG+
Sbjct: 532 GRYVIYVKAGIYREYITVTKDHVNVYMYGDGPRKTIVTGTKCYRDGITTYKTATFSAIGK 591

Query: 301 GFLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISG 360
           GF+ +SMGF NTAGP+GHQAVALRVQSD SAFFNCRMDGYQDTLYVQ  RQFYRNCVISG
Sbjct: 592 GFVARSMGFVNTAGPDGHQAVALRVQSDMSAFFNCRMDGYQDTLYVQAHRQFYRNCVISG 651

Query: 361 TVDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFA 420
           T+DFIFGDSTTVIQNSLIIVRRP D QQNTVTA G+ + +E +GLVIH+CRIVPEQKLF 
Sbjct: 652 TIDFIFGDSTTVIQNSLIIVRRPNDKQQNTVTAQGKTEKRETTGLVIHDCRIVPEQKLFP 711

Query: 421 ERFKIPTFLGRPWKEYARTVIMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAV 480
           +RFKIP+FLGRPWK Y++T+IME+T+GDFIQP G+ PW+G F   T LY EYGN GPGA 
Sbjct: 712 DRFKIPSFLGRPWKPYSKTIIMETTLGDFIQPAGWTPWAGKFVPNTLLYAEYGNLGPGAN 771

Query: 481 TNRRVRWKGVKVI-GRNEAMQFTAGSFLQGKTWLPTTGGPYLLGLK 525
           T+ RV WKG ++I  RNEA+Q+T  SF+QG  WL     PYL GLK
Sbjct: 772 THSRVTWKGYRIIKTRNEALQYTVNSFIQGNLWLKQINIPYLPGLK 813

BLAST of Cucsa.374170 vs. TrEMBL
Match: F6HZG5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_07s0005g02750 PE=4 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 1.3e-103
Identity = 181/237 (76.37%), Postives = 207/237 (87.34%), Query Frame = 1

Query: 266 MYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQ 325
           MYGDGP+KTIVTG KS R G TT  TATF AIG+GF+ +SMGF NTAGP+GHQAVALRVQ
Sbjct: 1   MYGDGPRKTIVTGTKSYRDGITTYKTATFSAIGKGFVARSMGFVNTAGPDGHQAVALRVQ 60

Query: 326 SDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIVRRPMDN 385
           SD SA FNCRMDGYQDTLY+Q  RQFYRNCVISGT+DFIFGDSTTVIQNSLIIVRRP DN
Sbjct: 61  SDMSAIFNCRMDGYQDTLYIQAHRQFYRNCVISGTIDFIFGDSTTVIQNSLIIVRRPKDN 120

Query: 386 QQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTM 445
           QQNTVTA+G+A+ +E +GLVIHNCRIVPEQKLF +RFKIP+FLGRPWK Y++T+IME+T+
Sbjct: 121 QQNTVTAHGKAEKRETTGLVIHNCRIVPEQKLFPDRFKIPSFLGRPWKPYSKTIIMETTL 180

Query: 446 GDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVI-GRNEAMQFT 502
           GDFIQP G+MPW+G+FAL T  Y EYGNRGPGA T  RV WKG ++I  RNEA+Q+T
Sbjct: 181 GDFIQPAGWMPWAGDFALNTLFYAEYGNRGPGANTRSRVTWKGYRIIKTRNEALQYT 237

HSP 2 Score: 662.1 bits (1707), Expect = 5.5e-187
Identity = 331/525 (63.05%), Postives = 405/525 (77.14%), Query Frame = 1

Query: 2   KAVSTICSTANYQEECQNTLTNAAHNASSDDPKEYVKAAILATIDEVKKGYNLTDGFLIE 61
           KAV+ ICS  +Y+++C ++    A+N S+  PK+++ AAI  T+ EVK+    ++   + 
Sbjct: 49  KAVAAICSPTDYKQQCISSFQTLANNHSAT-PKDFLMAAIDITMKEVKEAIGKSESIGLA 108

Query: 62  AANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQS 121
             ++   KM  EDC+DLLQ AI +LQAS+S VG+  +HT  DR  D+KNWL++VISYQQS
Sbjct: 109 GNSSGRQKMATEDCQDLLQLAIGELQASFSMVGDAAMHTINDRAQDLKNWLSAVISYQQS 168

Query: 122 CLDGLEEFDPQLRQKMQDGLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGT 181
           C+DG +E  P+++  +Q+GL  A +LTSNALAIV  +S IL SF + L    S RRL   
Sbjct: 169 CMDGFDE-TPEVKSAIQNGLLNATQLTSNALAIVSEISAILTSFNIPLNFTSSFRRLQEA 228

Query: 182 TEVDNDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRGR 241
           TE ++D YP+W + ADRKLL     G KV PNAVVA DGSGQ+K+I AALAAYPK L GR
Sbjct: 229 TE-ESDQYPSWFSAADRKLLGRVDNG-KVTPNAVVALDGSGQYKSIGAALAAYPKNLNGR 288

Query: 242 YVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGF 301
           YVIYVKAGIY EY+ I K++ NIFMYGDGP+KT+VTG KS   G TT  T+TF  IG GF
Sbjct: 289 YVIYVKAGIYDEYITIEKNLVNIFMYGDGPRKTMVTGKKSFLDGITTYKTSTFSVIGNGF 348

Query: 302 LCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTV 361
           +CKSMGF+NTAGPEGHQAVALRVQSD SAFFNCRMDGYQDTLYVQ  RQFYRNCVISGTV
Sbjct: 349 ICKSMGFRNTAGPEGHQAVALRVQSDYSAFFNCRMDGYQDTLYVQAHRQFYRNCVISGTV 408

Query: 362 DFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAER 421
           DFIFGDSTT+IQNSLIIVR+P+DNQQNT+TA+GRA+ +E +GLVI NCRIVPE+KLF  R
Sbjct: 409 DFIFGDSTTLIQNSLIIVRKPLDNQQNTITAHGRAEKRETTGLVIQNCRIVPEEKLFPTR 468

Query: 422 FKIPTFLGRPWKEYARTVIMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTN 481
           FK P++LGRPWK Y+RTV+MESTMGDFI P G++PW GNFAL+T  Y EY N+GPGA T 
Sbjct: 469 FKTPSYLGRPWKAYSRTVVMESTMGDFIHPAGWLPWMGNFALDTLYYAEYANKGPGANTA 528

Query: 482 RRVRWKGVKVI-GRNEAMQFTAGSFLQGKTWLPTTGGPYLLGLKS 526
           +RV+WKG KVI  RNEA+QFTAG F+QG  WL  TG PY LG ++
Sbjct: 529 QRVKWKGYKVITNRNEALQFTAGPFIQGNEWLRLTGAPYFLGFRT 569

BLAST of Cucsa.374170 vs. TAIR10
Match: AT4G33230.1 (AT4G33230.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 448.0 bits (1151), Expect = 8.2e-126
Identity = 237/529 (44.80%), Postives = 337/529 (63.71%), Query Frame = 1

Query: 2   KAVSTICSTANYQEECQNTLTNAAH-NASSDDPKEYVKAAILATIDEVKKGYNLTDGFLI 61
           K + T+C++  Y+  CQNTL N    +    DP+  +K+AI+A  D++ + +        
Sbjct: 93  KIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQVFKRVLSLKT 152

Query: 62  EAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQ 121
           E   N+  K  +  C+ L+  A ++L  S   + + +++  A  V D+ +WL++V+SYQ+
Sbjct: 153 E---NKDDKDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDSWLSAVMSYQE 212

Query: 122 SCLDGLEEFDPQLRQKMQDGLNGAGKLTSNALAIVDAVSDILASFG-----LQLKAQPSG 181
           +C+DG EE   +L+ +++   N +  LTSN+LA++ ++   L+S       L L+A+ S 
Sbjct: 213 TCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVPKVKTRLLLEARSSA 272

Query: 182 RRLLGTTEVDNDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYP 241
           +        + D   +WL+  +R++L +    A +KPNA VAKDGSG F TI+AAL A P
Sbjct: 273 K--------ETDHITSWLSNKERRMLKAVDVKA-LKPNATVAKDGSGNFTTINAALKAMP 332

Query: 242 KTLRGRYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFI 301
              +GRY IY+K GIY E V I K   N+ M GDG +KTIVTGNKS+     T  TATF+
Sbjct: 333 AKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKKIRTFLTATFV 392

Query: 302 AIGEGFLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNC 361
           A GEGF+ +SMGF+NTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY  T RQ+YR+C
Sbjct: 393 AQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYAYTHRQYYRSC 452

Query: 362 VISGTVDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQ 421
           VI GTVDFIFGD+  + QN  I +R+ +  Q+NTVTA GR D  + +G VIHNC + P +
Sbjct: 453 VIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFVIHNCTVAPNE 512

Query: 422 KLFAERFKIPTFLGRPWKEYARTVIMESTMGDFIQPVGYMPW-SGNFALETCLYLEYGNR 481
            L   + +  ++LGRPWK ++RTV+MEST+ D I PVG++ W   +FA++T  Y EY N 
Sbjct: 513 DLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAIDTLSYAEYKND 572

Query: 482 GPGAVTNRRVRWKGVKVIGRNEAMQFTAGSFLQGKTWLPTTGGPYLLGL 524
           GP   T  RV+W G +V+ + EAM+FT G FLQG+ W+   G P  LGL
Sbjct: 573 GPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE-WIQAIGSPVKLGL 606

BLAST of Cucsa.374170 vs. TAIR10
Match: AT3G05610.1 (AT3G05610.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 442.2 bits (1136), Expect = 4.5e-124
Identity = 214/523 (40.92%), Postives = 326/523 (62.33%), Query Frame = 1

Query: 1   MKAVSTICSTANYQEECQNTLTNAAHNASSDDPKEYVKAAILATIDEVKKGYNLTDGFLI 60
           +KAV  +C+  +Y++ C++TL     N +  DP E VK A   T+ ++      +   ++
Sbjct: 55  VKAVKDVCAPTDYRKTCEDTLIKNGKNTT--DPMELVKTAFNVTMKQITDAAKKSQT-IM 114

Query: 61  EAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQ 120
           E   +   +M ++ C++L+ +A+D+L  S+  +G+ + H   + + +++ WL++ IS+++
Sbjct: 115 ELQKDSRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEE 174

Query: 121 SCLDGLEEFDPQLRQKMQDGLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLG 180
           +CL+G +       + M+  L  A +LT N LAI+  +S+ +    +Q+    S RRLL 
Sbjct: 175 TCLEGFQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQ--MQIPGLNS-RRLLA 234

Query: 181 TTEVDNDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRG 240
                 +G+P+W+    RKLL +    + VKP+ VVA+DGSGQ+KTI+ AL   PK    
Sbjct: 235 ------EGFPSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNT 294

Query: 241 RYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEG 300
            +V+++KAG+YKEYV + K M ++   GDGP KTI++GNK+ + G TT  TAT   +G  
Sbjct: 295 TFVVHIKAGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNY 354

Query: 301 FLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGT 360
           F+ K++GF+NTAG   HQAVA+RVQSD S FFNCR DGYQDTLY  + RQF+R+C ISGT
Sbjct: 355 FIAKNIGFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGT 414

Query: 361 VDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAE 420
           +DF+FGD+  V QN  ++VR+P+ NQ   +TA+GR DP+E +G V   C I  E    A 
Sbjct: 415 IDFLFGDAAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAV 474

Query: 421 RFKIPTFLGRPWKEYARTVIMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVT 480
           +     +LGRPWKEY+RT+IM + + DF+QP G+ PW G+F L+T  Y E  N GPG+  
Sbjct: 475 KETSKAYLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSAL 534

Query: 481 NRRVRWKGVKVIGRNEAMQFTAGSFLQGKTWLPTTGGPYLLGL 524
             RV W G+K +   + ++FT   ++QG  W+P  G PY  GL
Sbjct: 535 ANRVTWAGIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGL 565

BLAST of Cucsa.374170 vs. TAIR10
Match: AT2G26450.1 (AT2G26450.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 439.1 bits (1128), Expect = 3.8e-123
Identity = 231/524 (44.08%), Postives = 336/524 (64.12%), Query Frame = 1

Query: 2   KAVSTICSTANYQEECQNTLTNAAHNASS-DDPKEYVKAAILATIDEVKKGYNLTDGFLI 61
           K + T+CS+  Y + C+ TL N      + D+P  ++K+AI A  +++     + +  L 
Sbjct: 107 KIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDL---VLEKVLS 166

Query: 62  EAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQ 121
               N+  K  +E C+ L++ A ++  AS + +   ++++    V D+++WL++V+SYQ+
Sbjct: 167 LKTENQDDKDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQE 226

Query: 122 SCLDGLEEFDPQLRQKMQDGLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLG 181
           +CLDG EE +  L+ +++  +N +  LTSN+LA++   ++ L+     ++     R LL 
Sbjct: 227 TCLDGFEEGN--LKSEVKTSVNSSQVLTSNSLALIKTFTENLSPVMKVVE-----RHLL- 286

Query: 182 TTEVDNDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRG 241
                 D  P+W++  DR++L +    A +KPNA VAKDGSG F TI+ AL A P+   G
Sbjct: 287 ------DDIPSWVSNDDRRMLRAVDVKA-LKPNATVAKDGSGDFTTINDALRAMPEKYEG 346

Query: 242 RYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEG 301
           RY+IYVK GIY EYV + K   N+ M GDG +KTIVTGNKS+     T  TATF+A GEG
Sbjct: 347 RYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEG 406

Query: 302 FLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGT 361
           F+ +SMGF+NTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY  T RQ+YR+CVI GT
Sbjct: 407 FMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGT 466

Query: 362 VDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAE 421
           +DFIFGD+  + QN  I +R+ +  Q+NTVTA GR D  + +G V+HNC+I   + L   
Sbjct: 467 IDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPV 526

Query: 422 RFKIPTFLGRPWKEYARTVIMESTMGDFIQPVGYMPW-SGNFALETCLYLEYGNRGPGAV 481
           + +  ++LGRPWK Y+RT+IMES + + I PVG++ W   +FA++T  Y EY N+G    
Sbjct: 527 KEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGD 586

Query: 482 TNRRVRWKGVKVIGRNEAMQFTAGSFLQGKTWLPTTGGPYLLGL 524
           T  RV+W G KVI + EA+ +T G FLQG  W+  +G P  LGL
Sbjct: 587 TTSRVKWPGFKVINKEEALNYTVGPFLQG-DWISASGSPVKLGL 611

BLAST of Cucsa.374170 vs. TAIR10
Match: AT5G27870.1 (AT5G27870.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 436.4 bits (1121), Expect = 2.5e-122
Identity = 215/525 (40.95%), Postives = 327/525 (62.29%), Query Frame = 1

Query: 1   MKAVSTICSTANYQEECQNTLTNAAHNASSDDPKEYVKAAILATIDEVKKGYNLTDGFLI 60
           +KA+  +C+  +Y+E C++TL   A + S  DP E VK A  AT+ ++      +   +I
Sbjct: 54  VKAIKDVCAPTDYKETCEDTLRKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQT-MI 113

Query: 61  EAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQ 120
           E   +   KM ++ C++L+ +AI +L  S+  +G+ + H   + +  ++ WL++ IS++Q
Sbjct: 114 ELQKDPRAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQ 173

Query: 121 SCLDGLEEFDPQLRQKMQDGLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLG 180
           +CLDG +       + ++  L  A +LT N LA+V  +S+ L    +    + + RRLL 
Sbjct: 174 TCLDGFQGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIP---EMNSRRLL- 233

Query: 181 TTEVDNDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRG 240
                +  +P+W+    R+LL      ++VKP+ VVA+DGSGQ+KTI+ AL   PK    
Sbjct: 234 -----SQEFPSWMDARARRLL--NAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNT 293

Query: 241 RYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEG 300
            +V+++K GIYKEYV + + M ++   GDGP KT+++G+KS + G TT  TAT   +G+ 
Sbjct: 294 TFVVHIKEGIYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDH 353

Query: 301 FLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGT 360
           F+ K++ F+NTAG   HQAVA+RV +D S F+NC+ DGYQDTLY  + RQFYR+C ISGT
Sbjct: 354 FIAKNIAFENTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGT 413

Query: 361 VDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAE 420
           +DF+FGD+  V QN  ++VR+P+ NQ   +TA+GR DP+E +G V+  C IV E    A 
Sbjct: 414 IDFLFGDAAAVFQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAV 473

Query: 421 RFKIPTFLGRPWKEYARTVIMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVT 480
           + +  T+LGRPWKEY+RT+IM + + DF+ P G+ PW G F L T  Y E  N GPGA  
Sbjct: 474 KEQSKTYLGRPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAI 533

Query: 481 NRRVRWKGVKVIGRNEAMQFTAGSFLQGKTWLPTTGGPYLLGLKS 526
            +RV W G+K +   E ++FT   ++QG  W+P  G PY+LGL S
Sbjct: 534 TKRVTWPGIKKLSDEEILKFTPAQYIQGDAWIPGKGVPYILGLFS 564

BLAST of Cucsa.374170 vs. TAIR10
Match: AT2G47030.1 (AT2G47030.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 431.8 bits (1109), Expect = 6.1e-121
Identity = 247/564 (43.79%), Postives = 333/564 (59.04%), Query Frame = 1

Query: 1   MKAVSTICSTANYQEECQNTLTNAAHNASSDDPKEYVKAAILATIDEVKKGYNLTDGFLI 60
           MKAV  IC + + +  C  TL        S+DP + +KA +LAT DE+ K  N T    +
Sbjct: 38  MKAVQGICQSTSDKASCVKTL----EPVKSEDPNKLIKAFMLATKDELTKSSNFTGQTEV 97

Query: 61  E-----AANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSV 120
                 + NN+++   ++ C+ +  +A++ L      +GE DL     ++  +K WL  V
Sbjct: 98  NMGSSISPNNKAV---LDYCKRVFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGV 157

Query: 121 ISYQQSCLDGLEEFDPQLRQKMQDGLNGAGKLTSNALAIVDAVSDILASFGLQLK----- 180
            +YQ  CLD +EE D  LR+ + +G+  +  LT+NA+ I   V   +A    ++      
Sbjct: 158 YNYQTDCLDDIEEDD--LRKAIGEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNM 217

Query: 181 ------------------AQPSG--RRLLGTTEVDNDGYPTWLTGADRKLLA-------- 240
                             A P G  RRLL   ++D  G PTW++GADRKL+A        
Sbjct: 218 TGGIPTPGAPPVVDESPVADPDGPARRLL--EDIDETGIPTWVSGADRKLMAKAGRGRRG 277

Query: 241 SRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRGRYVIYVKAGIYKEYVHITKDMK 300
            RGGGA+V+ N VVAKDGSGQFKT+  A+ A P+  RGR +IY+KAG+Y+E V I K   
Sbjct: 278 GRGGGARVRTNFVVAKDGSGQFKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKN 337

Query: 301 NIFMYGDGPKKTIVTGNKS--NRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAV 360
           NIFM+GDG +KT+++ N+S     G TT  +AT     EGF+ K MGF+NTAGP GHQA 
Sbjct: 338 NIFMFGDGARKTVISYNRSVALSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAA 397

Query: 361 ALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIVR 420
           A+RV  DR+  FNCR DGYQDTLYV   RQFYRNCV+SGTVDFIFG S TVIQN+LI+VR
Sbjct: 398 AIRVNGDRAVIFNCRFDGYQDTLYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVR 457

Query: 421 RPMDNQQNTVTANGRADPKEVS-GLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480
           +    Q NTVTA+G      +  G+V+ NCRIVP++KL  ER  + T+LGRPWK+++ TV
Sbjct: 458 KGSKGQYNTVTADGNELGLGMKIGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTV 517

Query: 481 IMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQ 524
           IM + MGD I+P G+  W G    ++C Y+EY NRGPGA  NRRV W  V      E   
Sbjct: 518 IMSTEMGDLIRPEGWKIWDGESFHKSCRYVEYNNRGPGAFANRRVNWAKV-ARSAAEVNG 577

BLAST of Cucsa.374170 vs. NCBI nr
Match: gi|449467649|ref|XP_004151535.1| (PREDICTED: pectinesterase-like [Cucumis sativus])

HSP 1 Score: 1061.2 bits (2743), Expect = 5.8e-307
Identity = 525/525 (100.00%), Postives = 525/525 (100.00%), Query Frame = 1

Query: 1   MKAVSTICSTANYQEECQNTLTNAAHNASSDDPKEYVKAAILATIDEVKKGYNLTDGFLI 60
           MKAVSTICSTANYQEECQNTLTNAAHNASSDDPKEYVKAAILATIDEVKKGYNLTDGFLI
Sbjct: 42  MKAVSTICSTANYQEECQNTLTNAAHNASSDDPKEYVKAAILATIDEVKKGYNLTDGFLI 101

Query: 61  EAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQ 120
           EAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQ
Sbjct: 102 EAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQ 161

Query: 121 SCLDGLEEFDPQLRQKMQDGLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLG 180
           SCLDGLEEFDPQLRQKMQDGLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLG
Sbjct: 162 SCLDGLEEFDPQLRQKMQDGLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLG 221

Query: 181 TTEVDNDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRG 240
           TTEVDNDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRG
Sbjct: 222 TTEVDNDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRG 281

Query: 241 RYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEG 300
           RYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEG
Sbjct: 282 RYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEG 341

Query: 301 FLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGT 360
           FLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGT
Sbjct: 342 FLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGT 401

Query: 361 VDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAE 420
           VDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAE
Sbjct: 402 VDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAE 461

Query: 421 RFKIPTFLGRPWKEYARTVIMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVT 480
           RFKIPTFLGRPWKEYARTVIMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVT
Sbjct: 462 RFKIPTFLGRPWKEYARTVIMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVT 521

Query: 481 NRRVRWKGVKVIGRNEAMQFTAGSFLQGKTWLPTTGGPYLLGLKS 526
           NRRVRWKGVKVIGRNEAMQFTAGSFLQGKTWLPTTGGPYLLGLKS
Sbjct: 522 NRRVRWKGVKVIGRNEAMQFTAGSFLQGKTWLPTTGGPYLLGLKS 566

BLAST of Cucsa.374170 vs. NCBI nr
Match: gi|659072601|ref|XP_008466374.1| (PREDICTED: pectinesterase-like isoform X1 [Cucumis melo])

HSP 1 Score: 1028.5 bits (2658), Expect = 4.2e-297
Identity = 504/525 (96.00%), Postives = 517/525 (98.48%), Query Frame = 1

Query: 1   MKAVSTICSTANYQEECQNTLTNAAHNASSDDPKEYVKAAILATIDEVKKGYNLTDGFLI 60
           MKAVSTIC+ ANYQ+ECQNTL+NAAHNASSDDPKEYV+AAIL+TI+EVKKGYNLTDGF+I
Sbjct: 43  MKAVSTICANANYQQECQNTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMI 102

Query: 61  EAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQ 120
           EAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQ
Sbjct: 103 EAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQ 162

Query: 121 SCLDGLEEFDPQLRQKMQDGLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLG 180
           SCLDGLEEFDPQL+QKMQDGL+ AGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLG
Sbjct: 163 SCLDGLEEFDPQLKQKMQDGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLG 222

Query: 181 TTEVDNDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRG 240
           TTEV  DGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPK LRG
Sbjct: 223 TTEVGKDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRG 282

Query: 241 RYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEG 300
           RYVIYVKAGIY EYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEG
Sbjct: 283 RYVIYVKAGIYNEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEG 342

Query: 301 FLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGT 360
           FLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGT
Sbjct: 343 FLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGT 402

Query: 361 VDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAE 420
           VDFIFGDSTT+IQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAE
Sbjct: 403 VDFIFGDSTTIIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAE 462

Query: 421 RFKIPTFLGRPWKEYARTVIMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVT 480
           RFKIPTFLGRPWK+YARTVIMEST+GDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVT
Sbjct: 463 RFKIPTFLGRPWKQYARTVIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVT 522

Query: 481 NRRVRWKGVKVIGRNEAMQFTAGSFLQGKTWLPTTGGPYLLGLKS 526
           NRRVRWKGVKVIGRNEAMQFTAG FLQG+ WLPTTGGPYLLGLKS
Sbjct: 523 NRRVRWKGVKVIGRNEAMQFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of Cucsa.374170 vs. NCBI nr
Match: gi|659072605|ref|XP_008466391.1| (PREDICTED: pectinesterase-like isoform X3 [Cucumis melo])

HSP 1 Score: 951.4 bits (2458), Expect = 6.5e-274
Identity = 460/525 (87.62%), Postives = 493/525 (93.90%), Query Frame = 1

Query: 1   MKAVSTICSTANYQEECQNTLTNAAHNASSDDPKEYVKAAILATIDEVKKGYNLTDGFLI 60
           MKAVS+ICS  +YQ+ECQ TL N  HN+S DDPKE++KAAI ATI+E+KKGYNLTD  ++
Sbjct: 41  MKAVSSICSNTDYQQECQTTLGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMV 100

Query: 61  EAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQ 120
           EAANN SIKM V+DCRDLLQ AID+L ASYSTVG+PDLHTN DRVADIKNWLT+VISYQQ
Sbjct: 101 EAANNASIKMTVDDCRDLLQSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQ 160

Query: 121 SCLDGLEEFDPQLRQKMQDGLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLG 180
           SCLDGLEEFDPQL+QKMQ GL+ A KLTSNALAIVDAVSDIL S GLQLK QPSGRRLLG
Sbjct: 161 SCLDGLEEFDPQLKQKMQGGLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLG 220

Query: 181 TTEVDNDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRG 240
           TTEVDNDG+PTWLTGADRKLLA++ GG +VKPNAVVAKDGSGQ+KTI+AALAAYPK L+G
Sbjct: 221 TTEVDNDGFPTWLTGADRKLLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKG 280

Query: 241 RYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEG 300
           RYVIYVKAGIY EY+ +TKDMKN+FMYGDGP+KTIVTG KSNR G  TQ+TATF+AIGEG
Sbjct: 281 RYVIYVKAGIYNEYIIVTKDMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFVAIGEG 340

Query: 301 FLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGT 360
           FLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGT
Sbjct: 341 FLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGT 400

Query: 361 VDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAE 420
           VDFIFGDSTT+IQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAE
Sbjct: 401 VDFIFGDSTTIIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAE 460

Query: 421 RFKIPTFLGRPWKEYARTVIMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVT 480
           RFKIPTFLGRPWK+YARTVIMEST+GDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVT
Sbjct: 461 RFKIPTFLGRPWKQYARTVIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVT 520

Query: 481 NRRVRWKGVKVIGRNEAMQFTAGSFLQGKTWLPTTGGPYLLGLKS 526
           NRRVRWKGVKVIGRNEAMQFTAG FLQG+ WLPTTGGPYLLGLKS
Sbjct: 521 NRRVRWKGVKVIGRNEAMQFTAGPFLQGRAWLPTTGGPYLLGLKS 565

BLAST of Cucsa.374170 vs. NCBI nr
Match: gi|659072603|ref|XP_008466382.1| (PREDICTED: pectinesterase-like isoform X2 [Cucumis melo])

HSP 1 Score: 918.3 bits (2372), Expect = 6.1e-264
Identity = 443/525 (84.38%), Postives = 480/525 (91.43%), Query Frame = 1

Query: 1   MKAVSTICSTANYQEECQNTLTNAAHNASSDDPKEYVKAAILATIDEVKKGYNLTDGFLI 60
           MKAVS+ICS  +YQ+ECQ TL N  HN+S DDPKE++KAAI ATI+E+KKGYNLTD  ++
Sbjct: 41  MKAVSSICSNTDYQQECQTTLGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMV 100

Query: 61  EAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQ 120
           EAANN SIKM V+DCRDLLQ AID+L ASYSTVG+PDLHTN DRVADIKNWLT+VISYQQ
Sbjct: 101 EAANNASIKMTVDDCRDLLQSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQ 160

Query: 121 SCLDGLEEFDPQLRQKMQDGLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLG 180
           SCLDGLEEFDPQL+QKMQ GL+ A KLTSNALAIVDAVSDIL S GLQLK QPSGRRLLG
Sbjct: 161 SCLDGLEEFDPQLKQKMQGGLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLG 220

Query: 181 TTEVDNDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRG 240
           TTEVDNDG+PTWLTGADRKLLA++ GG +VKPNAVVAKDGSGQ+KTI+AALAAYPK L+G
Sbjct: 221 TTEVDNDGFPTWLTGADRKLLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKG 280

Query: 241 RYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEG 300
           RYVIYVKAGIY EY+ +TKDMKN+FMYGDGP+KTIVTG KSNR G  TQ+TATF AIGEG
Sbjct: 281 RYVIYVKAGIYNEYIIVTKDMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEG 340

Query: 301 FLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGT 360
           FLCKSMGF NTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQT RQFYRNCV+SGT
Sbjct: 341 FLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGT 400

Query: 361 VDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAE 420
           VDFIFGDS T+IQNSLIIVRRPMDNQQNTVTANGRAD KE+SGLVIHNCRIVPEQKLFAE
Sbjct: 401 VDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAE 460

Query: 421 RFKIPTFLGRPWKEYARTVIMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVT 480
           RFKIPTFLGRPWKEYARTVIME+T+GDFIQP GYMPWSG+FAL TC Y EYGNRGPGA T
Sbjct: 461 RFKIPTFLGRPWKEYARTVIMETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANT 520

Query: 481 NRRVRWKGVKVIGRNEAMQFTAGSFLQGKTWLPTTGGPYLLGLKS 526
            RRVRWKGV+VIGRNEAMQFTAG FL G+ WLP TGGPYLLGLK+
Sbjct: 521 KRRVRWKGVRVIGRNEAMQFTAGPFLLGRAWLPATGGPYLLGLKN 565

BLAST of Cucsa.374170 vs. NCBI nr
Match: gi|659072607|ref|XP_008466403.1| (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 907.5 bits (2344), Expect = 1.1e-260
Identity = 435/525 (82.86%), Postives = 484/525 (92.19%), Query Frame = 1

Query: 1   MKAVSTICSTANYQEECQNTLTNAAHNASSDDPKEYVKAAILATIDEVKKGYNLTDGFLI 60
           MKA+S ICST +YQEEC+NTL + A N SS+DPK+Y KAAILATI E+ KGYNL+D  ++
Sbjct: 43  MKAISAICSTTDYQEECKNTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIV 102

Query: 61  EAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQ 120
           EA NN SIKM VEDC DLLQFAID+LQASYSTVGE DLHT+ADR+ADIKNWL++V+SYQQ
Sbjct: 103 EAGNNASIKMSVEDCNDLLQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQ 162

Query: 121 SCLDGLEEFDPQLRQKMQDGLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLG 180
           SCLDGL EFDPQL+Q+MQDGL+ AGKLTSNALAI+ AVS+IL ++GLQLK QPSGRRLLG
Sbjct: 163 SCLDGLGEFDPQLKQRMQDGLDVAGKLTSNALAILTAVSNILDNYGLQLKVQPSGRRLLG 222

Query: 181 TTEVDNDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRG 240
           TTEVD+DG+PTWLTGADRKLLAS+ GG +  PNAVVAKDGSGQ+KTI+AALAAYPK LRG
Sbjct: 223 TTEVDSDGFPTWLTGADRKLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRG 282

Query: 241 RYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEG 300
           RYVIYVKAGIY EY+ +TKDMKN+FMYGDGP+KTIVTG KSNR GFTTQ+TA+F AIGEG
Sbjct: 283 RYVIYVKAGIYDEYITVTKDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEG 342

Query: 301 FLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGT 360
           FLCKSMGF NTAGP+GHQAVALRVQSDRSAFFNCRMDG+QDTLYVQT RQFYRNCVISGT
Sbjct: 343 FLCKSMGFTNTAGPQGHQAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGT 402

Query: 361 VDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAE 420
           VDFIFGDS+T+IQNSLIIVRRPMDNQQNTVTA GRA+ KE++GLVIHNCRIVPEQKLFAE
Sbjct: 403 VDFIFGDSSTIIQNSLIIVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAE 462

Query: 421 RFKIPTFLGRPWKEYARTVIMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVT 480
           RFKIPTFLGRPWK+YARTVIMEST+GDFIQP GYMPWSGNFALETCLYLEYGNRGPGA T
Sbjct: 463 RFKIPTFLGRPWKQYARTVIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANT 522

Query: 481 NRRVRWKGVKVIGRNEAMQFTAGSFLQGKTWLPTTGGPYLLGLKS 526
           NRRVRWKGVKVIGRNEA+Q+TAG+FL G+TWLP TGG Y LGLK+
Sbjct: 523 NRRVRWKGVKVIGRNEALQYTAGAFLLGRTWLPRTGGIYYLGLKN 567