Cucsa.366700 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.366700
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Serine protease inhibitor
Location	scaffold03625 : 229832 .. 230220 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTGCTCTTTTATTAAAACTCGCTCTTACCCACCTTGCATAAGTTGTGGTTGCATGGATGCTTCATGTTGTAACAGTTAGGATCTTTTTTTTGTTATTTTGCTATCTCAATGATAATTTTTTAAAAAAAAAAAATTGAAAATTGGTTTATGTTTGTTATTAATTTTAGTGTTTCGTATTATTATGCAGAAGGCAAGTTTAAGTGGCCTGAAGTGGTAGGCATGAAGGGACAACAAGCTCAAAGTAAGATTGAAAAAGATGTATCTTTTGTGAGTGTTATATTGTTATCACCCGGAACAATTAGAATTGACAATTTTTGTTGCAATCGTGTTTTCGTTTACCTCGATGATAATGGCAAAGTCAATCAAATCCCAATCATTGGTTGA

mRNA sequence

ATGGCGTGCTCTTTTATTAAAACTCGCTCTTACCCACCTTGCATAAGTTGTGGTTGCATGGATGCTTCATGTTGTAACAAAGGCAAGTTTAAGTGGCCTGAAGTGGTAGGCATGAAGGGACAACAAGCTCAAAGTAAGATTGAAAAAGATGTATCTTTTGTGAGTGTTATATTGTTATCACCCGGAACAATTAGAATTGACAATTTTTGTTGCAATCGTGTTTTCGTTTACCTCGATGATAATGGCAAAGTCAATCAAATCCCAATCATTGGTTGA

Coding sequence (CDS)

ATGGCGTGCTCTTTTATTAAAACTCGCTCTTACCCACCTTGCATAAGTTGTGGTTGCATGGATGCTTCATGTTGTAACAAAGGCAAGTTTAAGTGGCCTGAAGTGGTAGGCATGAAGGGACAACAAGCTCAAAGTAAGATTGAAAAAGATGTATCTTTTGTGAGTGTTATATTGTTATCACCCGGAACAATTAGAATTGACAATTTTTGTTGCAATCGTGTTTTCGTTTACCTCGATGATAATGGCAAAGTCAATCAAATCCCAATCATTGGTTGA

Protein sequence

MACSFIKTRSYPPCISCGCMDASCCNKGKFKWPEVVGMKGQQAQSKIEKDVSFVSVILLSPGTIRIDNFCCNRVFVYLDDNGKVNQIPIIG*

Homology

[Close]

BLAST of Cucsa.366700 vs. TrEMBL
Match: Q9STF8_ARATH (PR-6 proteinase inhibitor family protein OS=Arabidopsis thaliana GN=T6H20.110 PE=2 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 6.7e-16
Identity = 44/82 (53.66%), Postives = 54/82 (65.85%), Query Frame = 1


Query: 11 YPPCISCGCMDASCCNKGK-FKWPEVVGMKGQQAQSKIEKDVSFVSVILLSPGTIRIDNF 70
          YPPC S  C D  CC  GK ++WPE+VG  GQ A+  IE++   V  I+L  G  RI+NF
Sbjct: 4  YPPCWSGSCEDPECCAIGKKYRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENF 63

Query: 71 CCNRVFVYLDDNGKVNQIPIIG 92
          CCNRVFVYL  NG+V   P+IG
Sbjct: 64 CCNRVFVYLGSNGQVADAPMIG 85

[Close]

BLAST of Cucsa.366700 vs. TrEMBL
Match: A0MF11_ARATH (Putative uncharacterized protein (Fragment) OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 1.7e-14
Identity = 43/82 (52.44%), Postives = 53/82 (64.63%), Query Frame = 1


Query: 11 YPPCISCGCMDASCCNKGK-FKWPEVVGMKGQQAQSKIEKDVSFVSVILLSPGTIRIDNF 70
          YPPC S  C D  C   GK ++WPE+VG  GQ A+  IE++   V  I+L  G  RI+NF
Sbjct: 4  YPPCWSGSCEDPECRAIGKKYRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENF 63

Query: 71 CCNRVFVYLDDNGKVNQIPIIG 92
          CCNRVFVYL  NG+V   P+IG
Sbjct: 64 CCNRVFVYLGSNGQVADAPMIG 85

[Close]

BLAST of Cucsa.366700 vs. TrEMBL
Match: V4NI49_EUTSA (Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10011055mg PE=4 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 3.7e-14
Identity = 40/82 (48.78%), Postives = 53/82 (64.63%), Query Frame = 1


Query: 11 YPPCISCGCMDASCCNKG-KFKWPEVVGMKGQQAQSKIEKDVSFVSVILLSPGTIRIDNF 70
          YPPC S GC D  CC  G KF+WPE+VG  G+ A+  IE++   V   ++  GT+RI++F
Sbjct: 7  YPPCESSGCTDPRCCALGGKFEWPELVGKSGEIAKMTIERENPNVLGFIMPYGTLRIEDF 66

Query: 71 CCNRVFVYLDDNGKVNQIPIIG 92
          CCNRVF+ +   G V  IP IG
Sbjct: 67 CCNRVFIVVGSKGHVVMIPKIG 88

[Close]

BLAST of Cucsa.366700 vs. TrEMBL
Match: A0A0D2TFV2_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_009G297200 PE=4 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 4.8e-14
Identity = 39/82 (47.56%), Postives = 52/82 (63.41%), Query Frame = 1


Query: 11 YPPCISCGCMDASCCNKG-KFKWPEVVGMKGQQAQSKIEKDVSFVSVILLSPGTIRIDNF 70
          YPPC S  C D  CC  G K +WPE++G  G  A++ IEK+   V+  +++PG +   NF
Sbjct: 7  YPPCASGTCTDVVCCAHGHKLRWPELLGENGAAAKATIEKENPEVTAEIVTPGRVGPPNF 66

Query: 71 CCNRVFVYLDDNGKVNQIPIIG 92
          CCNRVFV +D +G V  IP IG
Sbjct: 67 CCNRVFVIVDTHGNVTNIPTIG 88

[Close]

BLAST of Cucsa.366700 vs. TrEMBL
Match: A0A0D2SAJ6_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_009G297400 PE=4 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 4.8e-14
Identity = 39/82 (47.56%), Postives = 52/82 (63.41%), Query Frame = 1


Query: 11 YPPCISCGCMDASCCNKG-KFKWPEVVGMKGQQAQSKIEKDVSFVSVILLSPGTIRIDNF 70
          YPPC S  C D  CC  G K +WPE++G  G  A++ IEK+   V+  +++PG +   NF
Sbjct: 7  YPPCASGTCTDVVCCAHGHKLRWPELLGENGAAAKATIEKENPEVTAEIVTPGRVGPPNF 66

Query: 71 CCNRVFVYLDDNGKVNQIPIIG 92
          CCNRVFV +D +G V  IP IG
Sbjct: 67 CCNRVFVIVDTHGNVTNIPTIG 88

[Close]

BLAST of Cucsa.366700 vs. TAIR10
Match: AT3G46860.1 (AT3G46860.1 Serine protease inhibitor, potato inhibitor I-type family protein)

HSP 1 Score: 91.3 bits (225), Expect = 3.4e-19
Identity = 44/82 (53.66%), Postives = 54/82 (65.85%), Query Frame = 1


Query: 11 YPPCISCGCMDASCCNKGK-FKWPEVVGMKGQQAQSKIEKDVSFVSVILLSPGTIRIDNF 70
          YPPC S  C D  CC  GK ++WPE+VG  GQ A+  IE++   V  I+L  G  RI+NF
Sbjct: 4  YPPCWSGSCEDPECCAIGKKYRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENF 63

Query: 71 CCNRVFVYLDDNGKVNQIPIIG 92
          CCNRVFVYL  NG+V   P+IG
Sbjct: 64 CCNRVFVYLGSNGQVADAPMIG 85

[Close]

BLAST of Cucsa.366700 vs. TAIR10
Match: AT5G43580.1 (AT5G43580.1 Serine protease inhibitor, potato inhibitor I-type family protein)

HSP 1 Score: 63.2 bits (152), Expect = 9.9e-11
Identity = 29/72 (40.28%), Postives = 48/72 (66.67%), Query Frame = 1


Query: 20 MDASCCNKGKFKWPEVVGMKGQQAQSKIEKDVSFVSVILLSPGTIRIDNFCCNRVFVYLD 79
          M + C + GK  WPE++G KG+ A+  IE++   +  +++  GT+  + F C+RV+V+++
Sbjct: 28 MASICEDPGKSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVN 87

Query: 80 DNGKVNQIPIIG 92
          D G V QIPIIG
Sbjct: 88 DCGIVVQIPIIG 99

[Close]

BLAST of Cucsa.366700 vs. NCBI nr
Match: gi|657973711|ref|XP_008378667.1| (PREDICTED: proteinase inhibitor [Malus domestica])

HSP 1 Score: 93.2 bits (230), Expect = 2.5e-16
Identity = 39/82 (47.56%), Postives = 59/82 (71.95%), Query Frame = 1


Query: 11 YPPCISCGCMDASCCNKG-KFKWPEVVGMKGQQAQSKIEKDVSFVSVILLSPGTIRIDNF 70
          YPPC+S GC +A+CC +  K+ WPE+VG    +A + I K+   V+V++L PG IR+DNF
Sbjct: 9  YPPCVSEGCTNAACCGQEFKYVWPELVGKPASEAAATISKNNPAVTVLILKPGFIRLDNF 68

Query: 71 CCNRVFVYLDDNGKVNQIPIIG 92
          C NRV+V+ D+ G V+++P +G
Sbjct: 69 CXNRVYVFTDEKGIVSEVPTVG 90

[Close]

BLAST of Cucsa.366700 vs. NCBI nr
Match: gi|15232657|ref|NP_190270.1| (PR-6 proteinase inhibitor family protein [Arabidopsis thaliana])

HSP 1 Score: 91.3 bits (225), Expect = 9.6e-16
Identity = 44/82 (53.66%), Postives = 54/82 (65.85%), Query Frame = 1


Query: 11 YPPCISCGCMDASCCNKGK-FKWPEVVGMKGQQAQSKIEKDVSFVSVILLSPGTIRIDNF 70
          YPPC S  C D  CC  GK ++WPE+VG  GQ A+  IE++   V  I+L  G  RI+NF
Sbjct: 4  YPPCWSGSCEDPECCAIGKKYRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENF 63

Query: 71 CCNRVFVYLDDNGKVNQIPIIG 92
          CCNRVFVYL  NG+V   P+IG
Sbjct: 64 CCNRVFVYLGSNGQVADAPMIG 85

[Close]

BLAST of Cucsa.366700 vs. NCBI nr
Match: gi|116831272|gb|ABK28590.1| (unknown [Arabidopsis thaliana])

HSP 1 Score: 86.7 bits (213), Expect = 2.4e-14
Identity = 43/82 (52.44%), Postives = 53/82 (64.63%), Query Frame = 1


Query: 11 YPPCISCGCMDASCCNKGK-FKWPEVVGMKGQQAQSKIEKDVSFVSVILLSPGTIRIDNF 70
          YPPC S  C D  C   GK ++WPE+VG  GQ A+  IE++   V  I+L  G  RI+NF
Sbjct: 4  YPPCWSGSCEDPECRAIGKKYRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENF 63

Query: 71 CCNRVFVYLDDNGKVNQIPIIG 92
          CCNRVFVYL  NG+V   P+IG
Sbjct: 64 CCNRVFVYLGSNGQVADAPMIG 85

[Close]

BLAST of Cucsa.366700 vs. NCBI nr
Match: gi|567189624|ref|XP_006404448.1| (hypothetical protein EUTSA_v10011055mg [Eutrema salsugineum])

HSP 1 Score: 85.5 bits (210), Expect = 5.3e-14
Identity = 40/82 (48.78%), Postives = 53/82 (64.63%), Query Frame = 1


Query: 11 YPPCISCGCMDASCCNKG-KFKWPEVVGMKGQQAQSKIEKDVSFVSVILLSPGTIRIDNF 70
          YPPC S GC D  CC  G KF+WPE+VG  G+ A+  IE++   V   ++  GT+RI++F
Sbjct: 7  YPPCESSGCTDPRCCALGGKFEWPELVGKSGEIAKMTIERENPNVLGFIMPYGTLRIEDF 66

Query: 71 CCNRVFVYLDDNGKVNQIPIIG 92
          CCNRVF+ +   G V  IP IG
Sbjct: 67 CCNRVFIVVGSKGHVVMIPKIG 88

[Close]

BLAST of Cucsa.366700 vs. NCBI nr
Match: gi|823220079|ref|XP_012442756.1| (PREDICTED: glu S.griseus protease inhibitor-like [Gossypium raimondii])

HSP 1 Score: 85.1 bits (209), Expect = 6.9e-14
Identity = 39/82 (47.56%), Postives = 52/82 (63.41%), Query Frame = 1


Query: 11 YPPCISCGCMDASCCNKG-KFKWPEVVGMKGQQAQSKIEKDVSFVSVILLSPGTIRIDNF 70
          YPPC S  C D  CC  G K +WPE++G  G  A++ IEK+   V+  +++PG +   NF
Sbjct: 7  YPPCASGTCTDVVCCAHGHKLRWPELLGENGAAAKATIEKENPEVTAEIVTPGRVGPPNF 66

Query: 71 CCNRVFVYLDDNGKVNQIPIIG 92
          CCNRVFV +D +G V  IP IG
Sbjct: 67 CCNRVFVIVDTHGNVTNIPTIG 88

The following BLAST results are available for this feature:

BLAST of Cucsa.366700 vs. TrEMBL


Match Name	E-value	Identity	Description
Q9STF8_ARATH	6.7e-16	53.66	PR-6 proteinase inhibitor family protein OS=Arabidopsis thaliana GN=T6H20.110 PE...	[more]
A0MF11_ARATH	1.7e-14	52.44	Putative uncharacterized protein (Fragment) OS=Arabidopsis thaliana PE=2 SV=1	[more]
V4NI49_EUTSA	3.7e-14	48.78	Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10011055mg PE=4 SV=1	[more]
A0A0D2TFV2_GOSRA	4.8e-14	47.56	Uncharacterized protein OS=Gossypium raimondii GN=B456_009G297200 PE=4 SV=1	[more]
A0A0D2SAJ6_GOSRA	4.8e-14	47.56	Uncharacterized protein OS=Gossypium raimondii GN=B456_009G297400 PE=4 SV=1	[more]

BLAST of Cucsa.366700 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G46860.1	3.4e-19	53.66	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43580.1	9.9e-11	40.28	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

BLAST of Cucsa.366700 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|657973711\|ref\|XP_008378667.1\|	2.5e-16	47.56	PREDICTED: proteinase inhibitor [Malus domestica]	[more]
gi\|15232657\|ref\|NP_190270.1\|	9.6e-16	53.66	PR-6 proteinase inhibitor family protein [Arabidopsis thaliana]	[more]
gi\|116831272\|gb\|ABK28590.1\|	2.4e-14	52.44	unknown [Arabidopsis thaliana]	[more]
gi\|567189624\|ref\|XP_006404448.1\|	5.3e-14	48.78	hypothetical protein EUTSA_v10011055mg [Eutrema salsugineum]	[more]
gi\|823220079\|ref\|XP_012442756.1\|	6.9e-14	47.56	PREDICTED: glu S.griseus protease inhibitor-like [Gossypium raimondii]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000864	Prot_inh_pot1

Vocabulary: **Molecular Function**
Term	Definition
GO:0004867	serine-type endopeptidase inhibitor activity

Vocabulary: **Biological Process**
Term	Definition
GO:0009611	response to wounding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009611	response to wounding
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.366700.1	Cucsa.366700.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRODOM	PD002604		coord: 32..91 score: 5.
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 29..91 score: 3.1
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PROSITE	PS00285	POTATO_INHIBITOR	coord: 32..43 scor
IPR000864	Proteinase inhibitor I13, potato inhibitor I	unknown	SSF54654	CI-2 family of serine protease inhibitors	coord: 24..91 score: 1.24
None	No IPR available	GENE3D	G3DSA:3.30.10.10		coord: 25..91 score: 3.8
None	No IPR available	PANTHER	PTHR33091	FAMILY NOT NAMED	coord: 11..91 score: 7.4
None	No IPR available	PANTHER	PTHR33091:SF13	PR-6 PROTEINASE INHIBITOR FAMILY PROTEIN-RELATED	coord: 11..91 score: 7.4

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.366700	Cucumber (Gy14) v1	cgycgyB050
Cucsa.366700	Cucumber (Gy14) v1	cgycgyB051
Cucsa.366700	Cucurbita maxima (Rimu)	cgycmaB1023
Cucsa.366700	Cucurbita maxima (Rimu)	cgycmaB1015
Cucsa.366700	Cucurbita maxima (Rimu)	cgycmaB1021
Cucsa.366700	Cucurbita maxima (Rimu)	cgycmaB1022
Cucsa.366700	Cucurbita moschata (Rifu)	cgycmoB1015
Cucsa.366700	Cucurbita moschata (Rifu)	cgycmoB1020
Cucsa.366700	Cucurbita moschata (Rifu)	cgycmoB1021
Cucsa.366700	Wild cucumber (PI 183967)	cgycpiB542
Cucsa.366700	Cucumber (Chinese Long) v2	cgycuB513
Cucsa.366700	Melon (DHL92) v3.5.1	cgymeB596
Cucsa.366700	Melon (DHL92) v3.5.1	cgymeB597
Cucsa.366700	Melon (DHL92) v3.5.1	cgymeB598
Cucsa.366700	Watermelon (Charleston Gray)	cgywcgB629
Cucsa.366700	Watermelon (97103) v1	cgywmB667
Cucsa.366700	Cucurbita pepo (Zucchini)	cgycpeB0971
Cucsa.366700	Cucurbita pepo (Zucchini)	cgycpeB0973
Cucsa.366700	Cucurbita pepo (Zucchini)	cgycpeB0974
Cucsa.366700	Bottle gourd (USVL1VR-Ls)	cgylsiB595
Cucsa.366700	Bottle gourd (USVL1VR-Ls)	cgylsiB594
Cucsa.366700	Melon (DHL92) v3.6.1	cgymedB594
Cucsa.366700	Melon (DHL92) v3.6.1	cgymedB595
Cucsa.366700	Silver-seed gourd	carcgyB0924
Cucsa.366700	Silver-seed gourd	carcgyB0971
Cucsa.366700	Silver-seed gourd	carcgyB1178
Cucsa.366700	Cucumber (Chinese Long) v3	cgycucB557
Cucsa.366700	Cucumber (Chinese Long) v3	cgycucB558
Cucsa.366700	Watermelon (97103) v2	cgywmbB635
Cucsa.366700	Wax gourd	cgywgoB741