Cucsa.365270 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.365270 vs. Swiss-Prot
Match: DI3L2_ARATH (DIS3-like exonuclease 2 OS=Arabidopsis thaliana GN=SOV PE=1 SV=1)

HSP 1 Score: 983.0 bits (2540), Expect = 2.7e-285
Identity = 552/1131 (48.81%), Postives = 739/1131 (65.34%), Query Frame = 1

Query: 1    MRAAVEQSTPERNGDGDKEKKKK-RRSNRRSKHNPSLTTSASYTSVNGILGEASECMENG 60
            M++A  + + ER  +G K+K+ + ++ NRRSK +      A             E ++ G
Sbjct: 1    MKSASSEQSVERIENGHKKKRNRPQKQNRRSKQSSVPIEDA----------HVEESLD-G 60

Query: 61   RIDANLTSPSNYSSLTQQENHSNQQIEHGLTRGDKIGFSSLPPLHINEQAELSASHNLMN 120
            R D++ +   + +S ++Q+  +  ++E    R   + F+S+PP+         ++  L++
Sbjct: 61   R-DSSRSKAKDSTSSSKQQRPNTDELE--AMRASNVAFNSMPPMRAESGYPRRSASPLLS 120

Query: 121  QNHHSSDAGGRVTKSCPEQIASGRYSGISLNQHSPPADVTDNNTQRKYFPSHWSVDDVNE 180
                S      ++KSCP+  A  +  G++           + ++QRK F SHWS+D V E
Sbjct: 121  SPEVSKQL---LSKSCPDPRACEQSPGMNGELFQQ----IEGSSQRKIFSSHWSLDAVTE 180

Query: 181  GLQKGGIFKALFRVNAHNRLEAYCKIDGLPIDVLINGIASQNRAVEGDIVAIKLDPFTSW 240
             L+KG  FKALFRVNAHNR EAYCKIDG+P D+LING   Q+RAVEGD V IKLDP + W
Sbjct: 181  ALEKGEAFKALFRVNAHNRNEAYCKIDGVPTDILINGNVCQSRAVEGDTVVIKLDPLSLW 240

Query: 241  TKMKGTSEAHNNMHSMEDANLPAELTEKNDHNCKGKNKVDADVKSDSFRSTSLPDKRCCS 300
             KMKG      +    E  N P    EK+D   + KN +D     + F      +K    
Sbjct: 241  PKMKGF--VTESAAKPEGTNSPP---EKDDKKARQKNGIDV---VEGFEDGFSKNKSSVI 300

Query: 301  EDKVLDGV------ACDVLLSNYEQCDINELSVVNPSQAHHSSNQDDVSKAIGRICALIN 360
                 +GV      + D  L ++  C+                 Q     A+ ++C +++
Sbjct: 301  GKGAKNGVTPSSPPSLDSCLGSF--CE-----------------QKGNCSAVDKLCGILS 360

Query: 361  LYPAKRPTGRVVTILEKSRLRENVVGHLNVKKFLSFQEFYVKESTKSCLSPSQNCGYVQL 420
             +P KRPTG+VV ++EKS +R+++VG L+VK ++ ++E   K   KS LS S +  YVQL
Sbjct: 361  SFPHKRPTGQVVAVVEKSLVRDSIVGLLDVKGWIHYKESDPKRC-KSPLSLSDD-EYVQL 420

Query: 421  MPNDARFPIMMVLAGDLPNCIKKRLDNGDVTVENELVAARIYEWVKESSSPRAHVLHVLG 480
            MP D RFP ++V    LP  I+ RL+N D  +E ELVAA+I +W + S  P A + H+ G
Sbjct: 421  MPADPRFPKLIVPFHVLPGSIRARLENLDPNLEAELVAAQIVDWGEGSPFPVAQITHLFG 480

Query: 481  RGNEVESHIDAILFENAFRTCEFSQDSLSCVPQTPWKIPPEELQCRRDIRNLCIFTIDPS 540
            RG+E+E  I+AIL++N+    +FS  SL+ +P+ PW++P EE+Q R+D+R+LC+ TIDPS
Sbjct: 481  RGSELEPQINAILYQNSVCDSDFSPGSLTSLPRVPWEVPEEEVQRRKDLRDLCVLTIDPS 540

Query: 541  SASDLDDALSVQRLANGIFRVGIHIADVSYFVLPDTALDKEAQIRSTSVYLLQRKIPMLP 600
            +A+DLDDALSVQ L  G FRVG+HIADVSYFVLP+TALD EA+ RSTSVYL+QRKI MLP
Sbjct: 541  TATDLDDALSVQSLPGGFFRVGVHIADVSYFVLPETALDTEARFRSTSVYLMQRKISMLP 600

Query: 601  PLLSESIGSLNPGVDRLAFSLFLDINSCGDVKDFWIERTVICCCCKLSYEHAQDIIDGLI 660
            PLLSE++GSL+PG DRLAFS+  D+N  GDV D WI RT+I  CCKLSY+HAQDIIDG  
Sbjct: 601  PLLSENVGSLSPGADRLAFSILWDLNREGDVIDRWIGRTIIRSCCKLSYDHAQDIIDG-- 660

Query: 661  DSDSSELFGNNCPQLHGQFTWHDVISSVKLLHEISKTVKEKRFRNGALRLENSKLIYLYD 720
                S++  N  P LHG F W DV  SVK L EIS T+++KRFRNGAL+LENSK ++L+D
Sbjct: 661  ---KSDVAENGWPALHGSFKWCDVTRSVKQLSEISTTLRQKRFRNGALQLENSKPVFLFD 720

Query: 721  EYGIPYDSMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSALLRRHPEPMLRKLREFE 780
            E+G+PYD +   +K SNFLVEEFMLLAN T AEVIS+ +P S+LLRRHPEP  RKL+EFE
Sbjct: 721  EHGVPYDFVTCSRKGSNFLVEEFMLLANMTAAEVISQAYPASSLLRRHPEPNTRKLKEFE 780

Query: 781  TFCSKHGFELDTSSSVHFQQSLEQIRIELQDDPLLFDILISYATRPMQLATYFCSGELKD 840
             FCSKHG +LD SSS   Q SLE+I   L+DD +  DIL +YA +PMQLA+YFC+G LKD
Sbjct: 781  GFCSKHGMDLDISSSGQLQDSLEKITGNLKDDSVFVDILNNYAIKPMQLASYFCTGNLKD 840

Query: 841  GETR-SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKMYLKHKGVIQKVNSNEET 900
                  HYALAVPLYTHFTSPLRRYPDIVVHR LAAA+EAE++Y K K    +   +E  
Sbjct: 841  SVAEWGHYALAVPLYTHFTSPLRRYPDIVVHRALAAALEAEELYSKQK----QTAIDEGR 900

Query: 901  RCFTGIYFDKDAADSLEGREALSSAALKHGVPCSKLLLDVALHCNDRKLASKHVADGIEK 960
             CFTGI+F+KDAA+S+EG+EALS AALKHGVP +++L DVA +CN+RKLA++ V D  +K
Sbjct: 901  SCFTGIHFNKDAAESIEGKEALSVAALKHGVPSTEILSDVAAYCNERKLAARKVRDACDK 960

Query: 961  LYMWALLKKKKILFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLETTSTLV 1020
            LY W +LK+K+I   +ARV+ LG RFM+VYI KL IERRIYYD++EGL  +WLE TSTL+
Sbjct: 961  LYTWFVLKQKEIFPCEARVMNLGSRFMTVYISKLGIERRIYYDQIEGLCADWLEATSTLI 1020

Query: 1021 LRFFCSRRSHRSRGSVKWKALEDVALVISPCDQNVKERTLGVSSNGGASKGGSAVVEQDS 1080
            +    S+R  R      +K +++   ++SPC+  V +               SA+   D+
Sbjct: 1021 VDKLYSKRGGRG----FFKPMKEAVYLVSPCEVCVAK--------------CSALSVHDT 1054

Query: 1081 NLKSHVSDTGVDPAIFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSY 1124
                 VS   V PA+FPLT++L STIPV LHAVGGDDGP+DIG RLYMSSY
Sbjct: 1081 ESPEAVSIDEVAPAVFPLTIQLFSTIPVVLHAVGGDDGPLDIGARLYMSSY 1054

BLAST of Cucsa.365270 vs. Swiss-Prot
Match: DI32L_ARATH (Inactive exonuclease DIS3L2 OS=Arabidopsis thaliana GN=SOV PE=2 SV=1)

HSP 1 Score: 979.5 bits (2531), Expect = 3.0e-284
Identity = 551/1131 (48.72%), Postives = 738/1131 (65.25%), Query Frame = 1

Query: 1    MRAAVEQSTPERNGDGDKEKKKK-RRSNRRSKHNPSLTTSASYTSVNGILGEASECMENG 60
            M++A  + + ER  +G K+K+ + ++ NRRSK +      A             E ++ G
Sbjct: 1    MKSASSEQSVERIENGHKKKRNRPQKQNRRSKQSSVPIEDA----------HVEESLD-G 60

Query: 61   RIDANLTSPSNYSSLTQQENHSNQQIEHGLTRGDKIGFSSLPPLHINEQAELSASHNLMN 120
            R D++ +   + +S ++Q+  +  ++E    R   + F+S+PP+         ++  L++
Sbjct: 61   R-DSSRSKAKDSTSSSKQQRPNTDELE--AMRASNVAFNSMPPMRAESGYPRRSASPLLS 120

Query: 121  QNHHSSDAGGRVTKSCPEQIASGRYSGISLNQHSPPADVTDNNTQRKYFPSHWSVDDVNE 180
                S      ++KSCP+  A  +  G++           + ++QRK F SHWS+D V E
Sbjct: 121  SPEVSKQL---LSKSCPDPRACEQSPGMNGELFQQ----IEGSSQRKIFSSHWSLDAVTE 180

Query: 181  GLQKGGIFKALFRVNAHNRLEAYCKIDGLPIDVLINGIASQNRAVEGDIVAIKLDPFTSW 240
             L+KG  FKALFRVNAHNR EAYCKIDG+P D+LING   Q+RAVEGD V IKLDP + W
Sbjct: 181  ALEKGEAFKALFRVNAHNRNEAYCKIDGVPTDILINGNVCQSRAVEGDTVVIKLDPLSLW 240

Query: 241  TKMKGTSEAHNNMHSMEDANLPAELTEKNDHNCKGKNKVDADVKSDSFRSTSLPDKRCCS 300
             KMKG      +    E  N P    EK+D   + KN +D     + F      +K    
Sbjct: 241  PKMKGF--VTESAAKPEGTNSPP---EKDDKKARQKNGIDV---VEGFEDGFSKNKSSVI 300

Query: 301  EDKVLDGV------ACDVLLSNYEQCDINELSVVNPSQAHHSSNQDDVSKAIGRICALIN 360
                 +GV      + D  L ++  C+                 Q     A+ ++C +++
Sbjct: 301  GKGAKNGVTPSSPPSLDSCLGSF--CE-----------------QKGNCSAVDKLCGILS 360

Query: 361  LYPAKRPTGRVVTILEKSRLRENVVGHLNVKKFLSFQEFYVKESTKSCLSPSQNCGYVQL 420
             +P KRPTG+VV ++EKS +R+++VG L+VK ++ ++E   K   KS LS S +  YVQL
Sbjct: 361  SFPHKRPTGQVVAVVEKSLVRDSIVGLLDVKGWIHYKESDPKRC-KSPLSLSDD-EYVQL 420

Query: 421  MPNDARFPIMMVLAGDLPNCIKKRLDNGDVTVENELVAARIYEWVKESSSPRAHVLHVLG 480
            MP D RFP ++V    LP  I+ RL+N D  +E ELVAA+I +W + S  P A + H+ G
Sbjct: 421  MPADPRFPKLIVPFHVLPGSIRARLENLDPNLEAELVAAQIVDWGEGSPFPVAQITHLFG 480

Query: 481  RGNEVESHIDAILFENAFRTCEFSQDSLSCVPQTPWKIPPEELQCRRDIRNLCIFTIDPS 540
            RG+E+E  I+AIL++N+    +FS  SL+ +P+ PW++P EE+Q R+D+R+LC+ TIDPS
Sbjct: 481  RGSELEPQINAILYQNSVCDSDFSPGSLTSLPRVPWEVPEEEVQRRKDLRDLCVLTIDPS 540

Query: 541  SASDLDDALSVQRLANGIFRVGIHIADVSYFVLPDTALDKEAQIRSTSVYLLQRKIPMLP 600
            +A+DLDDALSVQ L  G FRVG+HIADVSYFVLP+TALD EA+ RSTSVYL+QRKI MLP
Sbjct: 541  TATDLDDALSVQSLPGGFFRVGVHIADVSYFVLPETALDTEARFRSTSVYLMQRKISMLP 600

Query: 601  PLLSESIGSLNPGVDRLAFSLFLDINSCGDVKDFWIERTVICCCCKLSYEHAQDIIDGLI 660
            PLLSE++GSL+PG DRLAFS+  D+N  GDV D WI RT+I  CCKLSY+HAQDIIDG  
Sbjct: 601  PLLSENVGSLSPGADRLAFSILWDLNREGDVIDRWIGRTIIRSCCKLSYDHAQDIIDG-- 660

Query: 661  DSDSSELFGNNCPQLHGQFTWHDVISSVKLLHEISKTVKEKRFRNGALRLENSKLIYLYD 720
                S++  N  P LHG F W DV  SVK L EIS T+++KRFRNGAL+LENSK ++L+D
Sbjct: 661  ---KSDVAENGWPALHGSFKWCDVTRSVKQLSEISTTLRQKRFRNGALQLENSKPVFLFD 720

Query: 721  EYGIPYDSMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSALLRRHPEPMLRKLREFE 780
            E+G+PYD +   +K SNFLVEEFMLLAN T AEVIS+ +  S+LLRRHPEP  RKL+EFE
Sbjct: 721  EHGVPYDFVTCSRKGSNFLVEEFMLLANMTAAEVISQAYRASSLLRRHPEPNTRKLKEFE 780

Query: 781  TFCSKHGFELDTSSSVHFQQSLEQIRIELQDDPLLFDILISYATRPMQLATYFCSGELKD 840
             FCSKHG +LD SSS   Q SLE+I   L+DD +  DIL +YA +PMQLA+YFC+G LKD
Sbjct: 781  GFCSKHGMDLDISSSGQLQDSLEKITGNLKDDSVFVDILNNYAIKPMQLASYFCTGNLKD 840

Query: 841  GETR-SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKMYLKHKGVIQKVNSNEET 900
                  HYALAVPLYTHFTSPLRRYPDIVVHR LAAA+EAE++Y K K    +   +E  
Sbjct: 841  SVAEWGHYALAVPLYTHFTSPLRRYPDIVVHRALAAALEAEELYSKQK----QTAIDEGR 900

Query: 901  RCFTGIYFDKDAADSLEGREALSSAALKHGVPCSKLLLDVALHCNDRKLASKHVADGIEK 960
             CFTGI+F+KDAA+S+EG+EALS AALKHGVP +++L DVA +CN+RKLA++ V D  +K
Sbjct: 901  SCFTGIHFNKDAAESIEGKEALSVAALKHGVPSTEILSDVAAYCNERKLAARKVRDACDK 960

Query: 961  LYMWALLKKKKILFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLETTSTLV 1020
            LY W +LK+K+I   +ARV+ LG RFM+VYI KL IERRIYYD++EGL  +WLE TSTL+
Sbjct: 961  LYTWFVLKQKEIFPCEARVMNLGSRFMTVYISKLGIERRIYYDQIEGLCADWLEATSTLI 1020

Query: 1021 LRFFCSRRSHRSRGSVKWKALEDVALVISPCDQNVKERTLGVSSNGGASKGGSAVVEQDS 1080
            +    S+R  R      +K +++   ++SPC+  V +               SA+   D+
Sbjct: 1021 VDKLYSKRGGRG----FFKPMKEAVYLVSPCEVCVAK--------------CSALSVHDT 1054

Query: 1081 NLKSHVSDTGVDPAIFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSY 1124
                 VS   V PA+FPLT++L STIPV LHAVGGDDGP+DIG RLYMSSY
Sbjct: 1081 ESPEAVSIDEVAPAVFPLTIQLFSTIPVVLHAVGGDDGPLDIGARLYMSSY 1054

BLAST of Cucsa.365270 vs. Swiss-Prot
Match: DI3L2_MOUSE (DIS3-like exonuclease 2 OS=Mus musculus GN=Dis3l2 PE=1 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 1.2e-112
Identity = 259/714 (36.27%), Postives = 387/714 (54.20%), Query Frame = 1

Query: 164 QRKYFPSHWSVDDVNEGLQKGGIFKALFRVNAHNRLEAYCKIDGLPIDVLINGIASQNRA 223
           ++  F ++ S +DV+EGL++G + + + R+N     EA+        D+ I+G+ ++NRA
Sbjct: 46  KKSIFETYMSKEDVSEGLKRGTLIQGVLRINPKKFHEAFIPSPDGDRDIFIDGVVARNRA 105

Query: 224 VEGDIVAIKLDPFTSWTKMKGTSEAHNNMHSMEDANLPAELTEKNDHNCKGKNKVDADVK 283
           + GD+V +KL P   W  +K  S     + +  +A++P E    +      K     DV 
Sbjct: 106 LNGDLVVVKLLPEDQWKAVKPESN-DKEIEATYEADIPEEGCGHHPLQQSRKGWSGPDVI 165

Query: 284 SDSFRSTSLPDKRCCSEDKVLDGVACDVLLSNYEQCDINELSVVNPSQAHHSSN---QDD 343
            ++    S  + R  +   ++DGV               +LS+  P +    S+     D
Sbjct: 166 IEAQFDDSDSEDRHGNTSGLVDGV--------------KKLSISTPDRGKEDSSTPVMKD 225

Query: 344 VSKAIGRICALINLYPAKRPTGRVVTILEK--SRLRENVVGHLNVKKFLSFQEFYVKEST 403
            +  I +    ++    ++ + +VV ILEK  SR    ++  L  K    F++       
Sbjct: 226 ENTPIPQDTRGLSEKSLQK-SAKVVYILEKKHSRAATGILKLLADKNSDLFKK------- 285

Query: 404 KSCLSPSQNCGYVQLMPNDARFPIMMVLAGDLPNCIKKRLDNGDVTVENELVAARIYEWV 463
                      Y    P+D R P + V   D P     R  +      N L   RI +W 
Sbjct: 286 -----------YALFSPSDHRVPRIYVPLKDCPQDFMTRPKD----FANTLFICRIIDWK 345

Query: 464 KESSSPRAHVLHVLGRGNEVESHIDAILFENAFRTCEFSQDSLSCVPQT-PWKIPPEELQ 523
           ++ +     +   LG+  E+E   + IL E      +FS + L C+PQ+ PW IPP+E+ 
Sbjct: 346 EDCNFALGQLAKSLGQAGEIEPETEGILTEYGVDFSDFSSEVLECLPQSLPWTIPPDEVG 405

Query: 524 CRRDIRNLCIFTIDPSSASDLDDALSVQRLANGIFRVGIHIADVSYFVLPDTALDKEAQI 583
            RRD+R  CIFTIDPS+A DLDDAL+ +RL +G F VG+HIADVSYFV   ++LDK A  
Sbjct: 406 KRRDLRKDCIFTIDPSTARDLDDALACRRLTDGTFEVGVHIADVSYFVPEGSSLDKVAAE 465

Query: 584 RSTSVYLLQRKIPMLPPLLSESIGSLNPGVDRLAFSLFLDINSCGDVKDFWIERTVICCC 643
           R+TSVYL+Q+ +PMLP LL E + SLNP  D+L FS+   +   G + + W  RT+I  C
Sbjct: 466 RATSVYLVQKVVPMLPRLLCEELCSLNPMTDKLTFSVIWKLTPEGKILEEWFGRTIIRSC 525

Query: 644 CKLSYEHAQDIIDGLIDSDSSELFGNNCPQLHGQFTWHDVISSVKLLHEISKTVKEKRFR 703
            KLSY+HAQ     +I++ + ++     P +  + +  +V  +V  LH I+K ++ +RF 
Sbjct: 526 TKLSYDHAQ----SMIENPTEKIPEEELPPISPEHSVEEVHQAVLNLHSIAKQLRRQRFV 585

Query: 704 NGALRLENSKLIYLYD-EYGIPYDSMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSA 763
           +GALRL+  KL +  D E G+P     YE +DSN LVEEFMLLAN  VA  I RTFP+ A
Sbjct: 586 DGALRLDQLKLAFTLDHETGLPQGCHIYEYRDSNKLVEEFMLLANMAVAHKIFRTFPEQA 645

Query: 764 LLRRHPEPMLRKLREFETFCSKHGFELDTSSSVHFQQSLEQIRIELQDDPLLFDILISYA 823
           LLRRHP P  + L +   FC + G  +D SS+    +SL +   + +      ++L +  
Sbjct: 646 LLRRHPPPQTKMLSDLVEFCDQMGLPMDVSSAGALNKSLTKTFGDDKYSLARKEVLTNMY 705

Query: 824 TRPMQLATYFCSGELKDGETRSHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAI 871
           +RPMQ+A YFCSG L+D E   HYAL VPLYTHFTSP+RR+ D++VHR LAAA+
Sbjct: 706 SRPMQMALYFCSGMLQDQEQFRHYALNVPLYTHFTSPIRRFADVIVHRLLAAAL 717

BLAST of Cucsa.365270 vs. Swiss-Prot
Match: DI3L2_HUMAN (DIS3-like exonuclease 2 OS=Homo sapiens GN=DIS3L2 PE=1 SV=4)

HSP 1 Score: 407.9 bits (1047), Expect = 3.6e-112
Identity = 262/714 (36.69%), Postives = 381/714 (53.36%), Query Frame = 1

Query: 164 QRKYFPSHWSVDDVNEGLQKGGIFKALFRVNAHNRLEAYCKIDGLPIDVLINGIASQNRA 223
           ++  F ++ S +DV+EGL++G + + + R+N     EA+        D+ I+G+ ++NRA
Sbjct: 46  KKSIFETYMSKEDVSEGLKRGTLIQGVLRINPKKFHEAFIPSPDGDRDIFIDGVVARNRA 105

Query: 224 VEGDIVAIKLDPFTSWTKMKGTSEAHNNMHSMEDANLPAELTEKN--DHNCKGKNKVDAD 283
           + GD+V +KL P   W  +K  S       + E +++P EL   +    + K  N     
Sbjct: 106 LNGDLVVVKLLPEEHWKVVKPESNDKETEAAYE-SDIPEELCGHHLPQQSLKSYNDSPDV 165

Query: 284 VKSDSFRSTSLPDKRCCSEDKVLDGVACDVLLSNYEQCDINELSVVNPSQAHHSSNQD-- 343
           +    F  +   D    +++ ++DGV               +LSV    +     +    
Sbjct: 166 IVEAQFDGSDSEDGHGITQNVLVDGV--------------KKLSVCVSEKGREDGDAPVT 225

Query: 344 -DVSKAIGRICALINLYPAKRPTGRVVTILEKSRLRENVVGHLNVKKFLSFQEFYVKEST 403
            D +  I +    ++    +R + +VV ILEK   R    G L +    + + F      
Sbjct: 226 KDETTCISQDTRALSEKSLQR-SAKVVYILEKKHSRA-ATGFLKLLADKNSELFRK---- 285

Query: 404 KSCLSPSQNCGYVQLMPNDARFPIMMVLAGDLPNCIKKRLDNGDVTVENELVAARIYEWV 463
                      Y    P+D R P + V   D P     R  +      N L   RI +W 
Sbjct: 286 -----------YALFSPSDHRVPRIYVPLKDCPQDFVARPKD----YANTLFICRIVDWK 345

Query: 464 KESSSPRAHVLHVLGRGNEVESHIDAILFENAFRTCEFSQDSLSCVPQ-TPWKIPPEELQ 523
           ++ +     +   LG+  E+E   + IL E      +FS + L C+PQ  PW IPPEE  
Sbjct: 346 EDCNFALGQLAKSLGQAGEIEPETEGILTEYGVDFSDFSSEVLECLPQGLPWTIPPEEFS 405

Query: 524 CRRDIRNLCIFTIDPSSASDLDDALSVQRLANGIFRVGIHIADVSYFVLPDTALDKEAQI 583
            RRD+R  CIFTIDPS+A DLDDALS + LA+G F+VG+HIADVSYFV   + LDK A  
Sbjct: 406 KRRDLRKDCIFTIDPSTARDLDDALSCKPLADGNFKVGVHIADVSYFVPEGSDLDKVAAE 465

Query: 584 RSTSVYLLQRKIPMLPPLLSESIGSLNPGVDRLAFSLFLDINSCGDVKDFWIERTVICCC 643
           R+TSVYL+Q+ +PMLP LL E + SLNP  D+L FS+   +   G + D W  RT+I  C
Sbjct: 466 RATSVYLVQKVVPMLPRLLCEELCSLNPMSDKLTFSVIWTLTPEGKILDEWFGRTIIRSC 525

Query: 644 CKLSYEHAQDIIDGLIDSDSSELFGNNCPQLHGQFTWHDVISSVKLLHEISKTVKEKRFR 703
            KLSYEHAQ     +I+S + ++     P +  + +  +V  +V  LH I+K ++++RF 
Sbjct: 526 TKLSYEHAQ----SMIESPTEKIPAKELPPISPEHSSEEVHQAVLNLHGIAKQLRQQRFV 585

Query: 704 NGALRLENSKLIYLYD-EYGIPYDSMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSA 763
           +GALRL+  KL +  D E G+P     YE ++SN LVEEFMLLAN  VA  I R FP+ A
Sbjct: 586 DGALRLDQLKLAFTLDHETGLPQGCHIYEYRESNKLVEEFMLLANMAVAHKIHRAFPEQA 645

Query: 764 LLRRHPEPMLRKLREFETFCSKHGFELDTSSSVHFQQSLEQIRIELQDDPLLFDILISYA 823
           LLRRHP P  R L +   FC + G  +D SS+    +SL Q   + +      ++L +  
Sbjct: 646 LLRRHPPPQTRMLSDLVEFCDQMGLPVDFSSAGALNKSLTQTFGDDKYSLARKEVLTNMC 705

Query: 824 TRPMQLATYFCSGELKDGETRSHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAI 871
           +RPMQ+A YFCSG L+D     HYAL VPLYTHFTSP+RR+ D++VHR LAAA+
Sbjct: 706 SRPMQMALYFCSGLLQDPAQFRHYALNVPLYTHFTSPIRRFADVLVHRLLAAAL 719

BLAST of Cucsa.365270 vs. Swiss-Prot
Match: DI3L2_XENTR (DIS3-like exonuclease 2 OS=Xenopus tropicalis GN=dis3l2 PE=2 SV=2)

HSP 1 Score: 371.7 bits (953), Expect = 2.8e-101
Identity = 211/473 (44.61%), Postives = 282/473 (59.62%), Query Frame = 1

Query: 415 PNDARFPIMMVLAGDLPNCIKKRLDNGDVTVENELVAARIYEWVKESSSPRAHVLHVLGR 474
           P D R P + V  GD P+      +    T  N L    I  W  +S+     ++  LG+
Sbjct: 241 PVDHRLPRIYVPLGDCPHDFAIHPE----TYANTLFICSITAWRDDSNFAEGKLMKSLGQ 300

Query: 475 GNEVESHIDAILFENAFRTCEFSQDSLSCVPQT-PWKIPPEELQCRRDIRNLCIFTIDPS 534
             E+E   + IL E      +F    L C+PQ  PW IP EE Q R+D+RN CIFTIDP+
Sbjct: 301 AGEIEPETEGILVEYGVDFSDFPDKVLQCLPQDLPWTIPQEEFQKRKDLRNECIFTIDPA 360

Query: 535 SASDLDDALSVQRLANGIFRVGIHIADVSYFVLPDTALDKEAQIRSTSVYLLQRKIPMLP 594
           +A DLDDALS + L +G F VG+HIADVSYFV   +ALD  A  R+TSVYL+Q+ IPMLP
Sbjct: 361 TARDLDDALSCKPLPDGNFEVGVHIADVSYFVAEGSALDIMASERATSVYLVQKVIPMLP 420

Query: 595 PLLSESIGSLNPGVDRLAFSLFLDINSCGDVKDFWIERTVICCCCKLSYEHAQDIIDGLI 654
            LL E + SLNP  DRL FS+   I   G++ D W  R+VIC C KLSY+HAQ+    +I
Sbjct: 421 RLLCEELCSLNPMTDRLTFSVIWKITPQGEILDEWFGRSVICSCVKLSYDHAQN----MI 480

Query: 655 DSDSSELFGNNCPQLHGQFTWHDVISSVKLLHEISKTVKEKRFRNGALRLENSKLIYLYD 714
           +    ++  +  P +  Q T +++  +V  LH I++ ++++RF +GALRL+  KL +  D
Sbjct: 481 NHPDKKIEQHELPPVSPQHTINEIHQAVLNLHLIAQNLRKQRFDDGALRLDQLKLTFTLD 540

Query: 715 -EYGIPYDSMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSALLRRHPEPMLRKLREF 774
            E G+P     Y+ +DSN LVEEFMLLAN  VA  I R FP+ ALLRRHP P  + L + 
Sbjct: 541 KESGLPQGCYIYQYRDSNKLVEEFMLLANMAVAHHIYRRFPEEALLRRHPPPQTKMLNDL 600

Query: 775 ETFCSKHGFELDTSSSVHFQQSLEQIRIELQDDPLLFDILISYATRPMQLATYFCSGELK 834
             FC + G +LD SSS    +SL       +      ++L +  +RPMQ+A YFC+G LK
Sbjct: 601 IEFCDQMGLQLDFSSSGTLHKSLNDQFETDEYSAARKEVLTNMCSRPMQMAVYFCTGALK 660

Query: 835 DGETRSHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKMYLKHKGVIQK 886
           D     HYAL VPLYTHFTSP+RR+ D++VHR LAA++         K VIQK
Sbjct: 661 DETLFHHYALNVPLYTHFTSPIRRFADVIVHRLLAASLGCGPPLKMPKEVIQK 705

HSP 2 Score: 52.4 bits (124), Expect = 3.8e-05
Identity = 29/107 (27.10%), Postives = 51/107 (47.66%), Query Frame = 1

Query: 164 QRKYFPSHWSVDDVNEGLQKGGIFKALFRVNAHNRLEAYCKIDGLPIDVLINGIASQNRA 223
           ++  F ++ + ++V+ GL++G + +   R+N     EAY        D+ I+G+  +NRA
Sbjct: 33  KKSVFEAYMTKEEVSAGLKRGELIQGPLRINPKKFHEAYLPSPDGVRDLFIDGVVPRNRA 92

Query: 224 VEGDIVAIKLDPFTSWTKMKGTSEAHNNMHSMEDANLPAELTEKNDH 271
           + GD+V +KL P   W  +K            ED + P   T    H
Sbjct: 93  LNGDVVVVKLLPQEQWKVLKN--------DVCEDDDTPGHSTGNKQH 131

HSP 3 Score: 42.7 bits (99), Expect = 3.0e-02
Identity = 27/83 (32.53%), Postives = 45/83 (54.22%), Query Frame = 1

Query: 914 LSSAALKHGVPCS---KLLLDVALHCNDRKLASKHVADGIEKLYMWALLKKKKILFSDAR 973
           L +A+L  G P     +++   A HCNDRK ASK V +   +L+    +K+   L S+A 
Sbjct: 685 LLAASLGCGPPLKMPKEVIQKQADHCNDRKTASKRVQELSAELFFSVFVKECGPLESEAM 744

Query: 974 VLGLGPRFMSVYIQKLAIERRIY 994
           V+G+      V + +  +++RIY
Sbjct: 745 VMGVLNEAFDVIVLRFGVQKRIY 767

BLAST of Cucsa.365270 vs. TrEMBL
Match: A0A0A0KC80_CUCSA (DIS3-like exonuclease 2 OS=Cucumis sativus GN=Csa_6G040560 PE=3 SV=1)

HSP 1 Score: 2260.3 bits (5856), Expect = 0.0e+00
Identity = 1124/1125 (99.91%), Postives = 1124/1125 (99.91%), Query Frame = 1

Query: 1    MRAAVEQSTPERNGDGDKEKKKKRRSNRRSKHNPSLTTSASYTSVNGILGEASECMENGR 60
            MRAAVEQSTPERNGDGDKEKKKKRRSNRRSKHNPSLTTSASYTSVNGILGEASECMENGR
Sbjct: 1    MRAAVEQSTPERNGDGDKEKKKKRRSNRRSKHNPSLTTSASYTSVNGILGEASECMENGR 60

Query: 61   IDANLTSPSNYSSLTQQENHSNQQIEHGLTRGDKIGFSSLPPLHINEQAELSASHNLMNQ 120
            IDANLTSPSNYSSLTQQENHSNQQIEHGLTRGDKIGFSSLPPLHINEQAELSASHNLMNQ
Sbjct: 61   IDANLTSPSNYSSLTQQENHSNQQIEHGLTRGDKIGFSSLPPLHINEQAELSASHNLMNQ 120

Query: 121  NHHSSDAGGRVTKSCPEQIASGRYSGISLNQHSPPADVTDNNTQRKYFPSHWSVDDVNEG 180
            NHHSSDAGGRVTKSCPEQIASGRYSGISLNQHSPPADVTDNNTQRKYFPSHWSVDDVNEG
Sbjct: 121  NHHSSDAGGRVTKSCPEQIASGRYSGISLNQHSPPADVTDNNTQRKYFPSHWSVDDVNEG 180

Query: 181  LQKGGIFKALFRVNAHNRLEAYCKIDGLPIDVLINGIASQNRAVEGDIVAIKLDPFTSWT 240
            LQKGGIFKALFRVNAHNRLEAYCKIDGLPIDVLINGIASQNRAVEGDIVAIKLDPFTSWT
Sbjct: 181  LQKGGIFKALFRVNAHNRLEAYCKIDGLPIDVLINGIASQNRAVEGDIVAIKLDPFTSWT 240

Query: 241  KMKGTSEAHNNMHSMEDANLPAELTEKNDHNCKGKNKVDADVKSDSFRSTSLPDKRCCSE 300
            KMKGTSEAHNNMHSMEDANLPAELTEKNDHNCKGKNKVDADVKSDSFRSTSLPDKRCCSE
Sbjct: 241  KMKGTSEAHNNMHSMEDANLPAELTEKNDHNCKGKNKVDADVKSDSFRSTSLPDKRCCSE 300

Query: 301  DKVLDGVACDVLLSNYEQCDINELSVVNPSQAHHSSNQDDVSKAIGRICALINLYPAKRP 360
            DKVLDGVACDVLLSNYEQCDINELSVVNPSQAHHSSNQDDVSKAIGRICALINLYPAKRP
Sbjct: 301  DKVLDGVACDVLLSNYEQCDINELSVVNPSQAHHSSNQDDVSKAIGRICALINLYPAKRP 360

Query: 361  TGRVVTILEKSRLRENVVGHLNVKKFLSFQEFYVKESTKSCLSPSQNCGYVQLMPNDARF 420
            TGRVVTILEKSRLRENVVGHLNVKKFLSFQEFYVKESTKSCLSPSQNCGYVQLMPNDARF
Sbjct: 361  TGRVVTILEKSRLRENVVGHLNVKKFLSFQEFYVKESTKSCLSPSQNCGYVQLMPNDARF 420

Query: 421  PIMMVLAGDLPNCIKKRLDNGDVTVENELVAARIYEWVKESSSPRAHVLHVLGRGNEVES 480
            PIMMVLAGDLPNCIKKRLDNGDVTVENELVAARIYEWVKESSSPRAHVLHVLGRGNEVES
Sbjct: 421  PIMMVLAGDLPNCIKKRLDNGDVTVENELVAARIYEWVKESSSPRAHVLHVLGRGNEVES 480

Query: 481  HIDAILFENAFRTCEFSQDSLSCVPQTPWKIPPEELQCRRDIRNLCIFTIDPSSASDLDD 540
            HIDAILFENA RTCEFSQDSLSCVPQTPWKIPPEELQCRRDIRNLCIFTIDPSSASDLDD
Sbjct: 481  HIDAILFENAIRTCEFSQDSLSCVPQTPWKIPPEELQCRRDIRNLCIFTIDPSSASDLDD 540

Query: 541  ALSVQRLANGIFRVGIHIADVSYFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLSESI 600
            ALSVQRLANGIFRVGIHIADVSYFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLSESI
Sbjct: 541  ALSVQRLANGIFRVGIHIADVSYFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLSESI 600

Query: 601  GSLNPGVDRLAFSLFLDINSCGDVKDFWIERTVICCCCKLSYEHAQDIIDGLIDSDSSEL 660
            GSLNPGVDRLAFSLFLDINSCGDVKDFWIERTVICCCCKLSYEHAQDIIDGLIDSDSSEL
Sbjct: 601  GSLNPGVDRLAFSLFLDINSCGDVKDFWIERTVICCCCKLSYEHAQDIIDGLIDSDSSEL 660

Query: 661  FGNNCPQLHGQFTWHDVISSVKLLHEISKTVKEKRFRNGALRLENSKLIYLYDEYGIPYD 720
            FGNNCPQLHGQFTWHDVISSVKLLHEISKTVKEKRFRNGALRLENSKLIYLYDEYGIPYD
Sbjct: 661  FGNNCPQLHGQFTWHDVISSVKLLHEISKTVKEKRFRNGALRLENSKLIYLYDEYGIPYD 720

Query: 721  SMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSALLRRHPEPMLRKLREFETFCSKHG 780
            SMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSALLRRHPEPMLRKLREFETFCSKHG
Sbjct: 721  SMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSALLRRHPEPMLRKLREFETFCSKHG 780

Query: 781  FELDTSSSVHFQQSLEQIRIELQDDPLLFDILISYATRPMQLATYFCSGELKDGETRSHY 840
            FELDTSSSVHFQQSLEQIRIELQDDPLLFDILISYATRPMQLATYFCSGELKDGETRSHY
Sbjct: 781  FELDTSSSVHFQQSLEQIRIELQDDPLLFDILISYATRPMQLATYFCSGELKDGETRSHY 840

Query: 841  ALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKMYLKHKGVIQKVNSNEETRCFTGIYF 900
            ALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKMYLKHKGVIQKVNSNEETRCFTGIYF
Sbjct: 841  ALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKMYLKHKGVIQKVNSNEETRCFTGIYF 900

Query: 901  DKDAADSLEGREALSSAALKHGVPCSKLLLDVALHCNDRKLASKHVADGIEKLYMWALLK 960
            DKDAADSLEGREALSSAALKHGVPCSKLLLDVALHCNDRKLASKHVADGIEKLYMWALLK
Sbjct: 901  DKDAADSLEGREALSSAALKHGVPCSKLLLDVALHCNDRKLASKHVADGIEKLYMWALLK 960

Query: 961  KKKILFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLETTSTLVLRFFCSRR 1020
            KKKILFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLETTSTLVLRFFCSRR
Sbjct: 961  KKKILFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLETTSTLVLRFFCSRR 1020

Query: 1021 SHRSRGSVKWKALEDVALVISPCDQNVKERTLGVSSNGGASKGGSAVVEQDSNLKSHVSD 1080
            SHRSRGSVKWKALEDVALVISPCDQNVKERTLGVSSNGGASKGGSAVVEQDSNLKSHVSD
Sbjct: 1021 SHRSRGSVKWKALEDVALVISPCDQNVKERTLGVSSNGGASKGGSAVVEQDSNLKSHVSD 1080

Query: 1081 TGVDPAIFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSYLR 1126
            TGVDPAIFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSYLR
Sbjct: 1081 TGVDPAIFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSYLR 1125

BLAST of Cucsa.365270 vs. TrEMBL
Match: U5GKY2_POPTR (DIS3-like exonuclease 2 OS=Populus trichocarpa GN=POPTR_0002s08690g PE=3 SV=1)

HSP 1 Score: 1228.8 bits (3178), Expect = 0.0e+00
Identity = 660/1134 (58.20%), Postives = 823/1134 (72.57%), Query Frame = 1

Query: 1    MRAAVEQST------PERNGDGDKEKKK-KRRSNRRSKHNPSLTTSASYTSVNGILGEAS 60
            M+++ EQS        E +GD +KEKKK KRRSNRRSK N   + + +++S N + GE+S
Sbjct: 1    MKSSSEQSPVMVVVRSEDSGDREKEKKKNKRRSNRRSKQN---SPNPAFSSQNDLRGESS 60

Query: 61   ECMENGRIDANLTSPSNYSSLTQQENHSNQQIEHGLTRGDKIGFSSLPPLHINEQAELSA 120
              + NG      TS    SS  Q E   +   EHG T   +I +SS+P +H+NEQ E   
Sbjct: 61   VSVGNGGKTRCYTSSMGCSSSKQLELDLHVLSEHGPTTVSRIAYSSMPTMHVNEQQEDLV 120

Query: 121  SHNLMNQNHHSSDAGGRVTKSCPEQIASGRYSGISLNQHSPPADVTDNNTQRKYFPSHWS 180
              +L          G  + KSC E I  G   G  L  H       +   Q K F  +WS
Sbjct: 121  LSDL---------DGSMLAKSCTEPIVGGGPHGKLLPFHQ-----FEGQAQSKIFAPYWS 180

Query: 181  VDDVNEGLQKGGIFKALFRVNAHNRLEAYCKIDGLPIDVLINGIASQNRAVEGDIVAIKL 240
             + VNE L+KG +FK LFRVNAHNRLEAYCKI+G+P DVLI+GIA+QNRA++GD+V I++
Sbjct: 181  TETVNEALEKGDVFKVLFRVNAHNRLEAYCKIEGVPTDVLISGIAAQNRAMDGDVVVIEV 240

Query: 241  DPFTSWTKMKGTSEAHNNMHSMEDANLPAELTEKNDHNCKGKNKVDADVKSDSFRSTSLP 300
            DP + WTKMKG++   NN  + ED+NL  E   K    CKGK+K++ D++   F ++ +P
Sbjct: 241  DPLSFWTKMKGSNVPSNNP-TAEDSNLHLEANGKVGGGCKGKSKMNLDLECADFGNSLVP 300

Query: 301  DKRCCSEDKVLDG-VACDVLLSNYEQCDINELSVVNPSQAHHSSNQDDVSKAIGRICALI 360
             K    ++    G V  D  ++ Y Q      S    S A  S+ QD+VS ++GRICA++
Sbjct: 301  QKGIHYDETACAGEVVHDNYVNGYHQ------SASESSLAVPSTGQDEVSNSVGRICAML 360

Query: 361  NLYPAKRPTGRVVTILEKSRLRENVVGHLNVKKFLSFQEFYVKESTKSCLSPS-QNCGYV 420
            +LYP+KRPTGRVV I+EKS  R+ +VG LNVK++  ++E   + + K+  S S  N  Y+
Sbjct: 361  SLYPSKRPTGRVVAIVEKSPRRDVIVGFLNVKQWFYYREGCRQNAKKNKSSLSISNREYI 420

Query: 421  QLMPNDARFPIMMVLAGDLPNCIKKRLDNGDVTVENELVAARIYEWVKESSSPRAHVLHV 480
            ++MP D RFP +MVL   LP+CIKKRL+N D TVE ELVAA+I  W  +S  P AHV  +
Sbjct: 421  EMMPTDPRFPKLMVLVSVLPDCIKKRLENEDATVEMELVAAQIDNWSDKSPFPEAHVSCI 480

Query: 481  LGRGNEVESHIDAILFENAFRTCEFSQDSLSCVPQTPWKIPPEELQCRRDIRNLCIFTID 540
             GRG+E+ES I+AIL ENA    +FS +SLSC+P   W++P +E++ R+DIRNLCIFTID
Sbjct: 481  FGRGSEMESQINAILHENAICCSKFSPESLSCLPSNTWEVPKDEIENRKDIRNLCIFTID 540

Query: 541  PSSASDLDDALSVQRLANGIFRVGIHIADVSYFVLPDTALDKEAQIRSTSVYLLQRKIPM 600
            PSSA+DLDDALSVQ+L NG+ RVG+HIADVSYFVLPDTALD EAQ RSTSVY+L+RKIPM
Sbjct: 541  PSSATDLDDALSVQKLPNGLVRVGVHIADVSYFVLPDTALDMEAQFRSTSVYMLRRKIPM 600

Query: 601  LPPLLSESIGSLNPGVDRLAFSLFLDINSCGDVKDFWIERTVICCCCKLSYEHAQDIIDG 660
            LPPLLSE++GSLNPGVDRLAFS+F D NS G+V D WI+RTVI  CCKLSYEHAQ I+DG
Sbjct: 601  LPPLLSENLGSLNPGVDRLAFSIFWDFNSSGNVVDRWIDRTVIQSCCKLSYEHAQGIVDG 660

Query: 661  LIDSDSSELFGNNCPQLHGQFTWHDVISSVKLLHEISKTVKEKRFRNGALRLENSKLIYL 720
            +ID+++   FG++ PQLHG F W DVI SV  LHEISKT++EKRF NGALRLE+SK+++L
Sbjct: 661  MIDTETCNTFGDSLPQLHGHFEWADVIGSVVCLHEISKTLREKRFDNGALRLESSKIVFL 720

Query: 721  YDEYGIPYDSMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSALLRRHPEPMLRKLRE 780
            +DEYGIPYDS   E+KDSNF+VEEFMLLAN T AE+ISR FPDSALLRRHPEP +RKLRE
Sbjct: 721  FDEYGIPYDSSLCERKDSNFIVEEFMLLANFTAAEIISRAFPDSALLRRHPEPNMRKLRE 780

Query: 781  FETFCSKHGFELDTSSSVHFQQSLEQIRIELQDDPLLFDILISYATRPMQLATYFCSGEL 840
            FE FC KHG ELDTSS  +FQQSLE+I+ +L+DDP LF+ILI+YA+RPMQLATYFCSG+L
Sbjct: 781  FEAFCCKHGLELDTSSG-NFQQSLERIKEKLKDDPELFNILINYASRPMQLATYFCSGDL 840

Query: 841  KDG-ETRSHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKMYLKHKGVIQKVNSNE 900
            KD      HYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAE++Y+  + +  K    E
Sbjct: 841  KDNMNDWGHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEQLYMMDRRMSLKARPGE 900

Query: 901  E-TRCFTGIYFDKDAADSLEGREALSSAALKHGVPCSKLLLDVALHCNDRKLASKHVADG 960
            E TRCFTGI F KD A+S EG+EALS+AALKH +PC +LL  VA +CN+RKLAS+HV D 
Sbjct: 901  EGTRCFTGICFCKDVAESAEGKEALSAAALKHRIPCPELLSHVAAYCNERKLASRHVKDA 960

Query: 961  IEKLYMWALLKKKKILFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLETTS 1020
             +KLYMW  +K+K++L SDARVLGLGPRFMS+YI KLAIERRIYYDEVEGL VEWLE TS
Sbjct: 961  CDKLYMWVSVKRKEVLLSDARVLGLGPRFMSIYINKLAIERRIYYDEVEGLTVEWLEATS 1020

Query: 1021 TLVLRFFCSRRSHRSRGSVKWKALEDVALVISPCDQNVKERTLGVSSNGGASKGGSAVVE 1080
            TLVL    S+RS R  GS  +KAL +VA VI+P D N++        +  ++KG SA   
Sbjct: 1021 TLVLNICASKRSVRRAGSGYYKALGEVAWVINPYDHNLE-------PDMESTKGCSASQH 1080

Query: 1081 QDSNLKSHVSDTGVDPAIFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSY 1124
             D+ LKS      +DP++FPLTVRLLSTIPVALHA+GGDDGP DIGVRL+MSSY
Sbjct: 1081 SDAILKSE-----IDPSVFPLTVRLLSTIPVALHAIGGDDGPPDIGVRLFMSSY 1097

BLAST of Cucsa.365270 vs. TrEMBL
Match: A0A067K983_JATCU (DIS3-like exonuclease 2 OS=Jatropha curcas GN=JCGZ_12063 PE=3 SV=1)

HSP 1 Score: 1221.5 bits (3159), Expect = 0.0e+00
Identity = 656/1144 (57.34%), Postives = 822/1144 (71.85%), Query Frame = 1

Query: 1    MRAAVEQSTP------ERNGDGDKEKKK-KRRSNRRSKHNPSLTTSASYTSVNGILGEAS 60
            M+ A E S+       E  GD D+EKKK KRRSNRRSK N   + + + +S+N + GE S
Sbjct: 1    MKGATELSSSLVVERVEDGGDEDREKKKNKRRSNRRSKQN---SRNQASSSINEVRGELS 60

Query: 61   ECMENGRIDANLTSPSNYSSLTQQENHSNQQIEHGLTRGDKIGFSSLPPLHINEQAELSA 120
                NG    N TS  + SS  Q E  ++   E+ LT    + F+S+P +HI+EQ E   
Sbjct: 61   HSFGNGGKTKNHTSSMSCSSSRQPELDAHGPDENRLTSASNVAFNSMPIMHIHEQVEPLL 120

Query: 121  SHNLMNQNHHSSDAGGRV-TKSCPEQIASGRYSGISLNQHSPPADVTDNNTQRKYFPSHW 180
               L          GG+V + SCPE  A+G      +N+ +      D N + K F  +W
Sbjct: 121  PSAL----------GGQVFSNSCPEPFAAGVPFETCVNKDALTFYQFDGNARGKIFTPYW 180

Query: 181  SVDDVNEGLQKGGIFKALFRVNAHNRLEAYCKIDGLPIDVLINGIASQNRAVEGDIVAIK 240
            S D +NE L+KG  FKALF VNAHN++EAYCKIDG+  DVL++GIA+QNRAVEGD+V IK
Sbjct: 181  STDAINEALEKGDAFKALFHVNAHNKVEAYCKIDGVSTDVLVSGIAAQNRAVEGDVVVIK 240

Query: 241  LDPFTSWTKMKGTSEAHNNMHSMEDANLPAELTEKNDHNCKGKNKVDADVKSDSFRSTSL 300
            +DP   WTKMKG++    N+ S+ED+NL  E      H  K K+K++ D +  +  S S 
Sbjct: 241  VDPLPCWTKMKGSNGPSTNLSSVEDSNLAVEA-----HEYKHKSKME-DYECTNPGSFSP 300

Query: 301  PDKRCCSEDKVLDGVACDVLLSNYEQCDINELSVVNPSQ------AHHSSN-QDDVSKAI 360
            P K    ED    G   D  +       +   ++VN  Q      +H SS  Q+DV   +
Sbjct: 301  PQKGIHCED----GACIDEGVRQEWNGPVG-YNLVNGHQPSASDSSHFSSTGQNDVMNGV 360

Query: 361  GRICALINLYPAKRPTGRVVTILEKSRLRENVVGHLNVKKFLSFQEFYVKESTKSCLSPS 420
            GR+CA+IN YP+KRPTGRVV I+E+S  R+ +VG L VK++L ++E   K+  K+  S S
Sbjct: 361  GRLCAMINSYPSKRPTGRVVAIVERSPRRDAIVGFLYVKQWLFYREACKKDGKKNKNSLS 420

Query: 421  QNCG-YVQLMPNDARFPIMMVLAGDLPNCIKKRLDNGDVTVENELVAARIYEWVKESSSP 480
             +   Y+QL P D +FP MMVL   LP+ IKKRL+ GD TVE ELVAA+I  W ++S  P
Sbjct: 421  ISAHEYIQLTPTDPKFPKMMVLMKSLPDPIKKRLEEGDPTVEMELVAAQIDNWDEDSPFP 480

Query: 481  RAHVLHVLGRGNEVESHIDAILFENAFRTCEFSQDSLSCVPQTPWKIPPEELQCRRDIRN 540
            +AHV H+ G G+E+E  I+AIL+ENA R  +FS +SLSC+P   WK+P EE++ RRD+RN
Sbjct: 481  QAHVSHIFGWGSEMEPQINAILYENAIRCSDFSPESLSCLPCDAWKVPAEEIKIRRDLRN 540

Query: 541  LCIFTIDPSSASDLDDALSVQRLANGIFRVGIHIADVSYFVLPDTALDKEAQIRSTSVYL 600
            LCIFTIDPS+A+DLDDALSV+RL NG  RVG+HIADVSYFVLPDTALD EAQ RSTSVY+
Sbjct: 541  LCIFTIDPSTATDLDDALSVERLQNGTLRVGVHIADVSYFVLPDTALDIEAQSRSTSVYM 600

Query: 601  LQRKIPMLPPLLSESIGSLNPGVDRLAFSLFLDINSCGDVKDFWIERTVICCCCKLSYEH 660
            L+RK+PMLPPLLSE++GSL PGVDRLAFS+F D+N  GDV D WI RTVI  CCKLSYEH
Sbjct: 601  LRRKLPMLPPLLSENLGSLKPGVDRLAFSIFWDLNGAGDVIDRWIGRTVIRSCCKLSYEH 660

Query: 661  AQDIIDGLIDSDSSELFGNNCPQLHGQFTWHDVISSVKLLHEISKTVKEKRFRNGALRLE 720
            AQ ++DG+ID D+S  +GN  PQLHG F W DVI SVK LHEISKT++EKRF +GAL+LE
Sbjct: 661  AQYMVDGMIDEDASSTYGNGLPQLHGPFEWADVIRSVKSLHEISKTLREKRFDDGALQLE 720

Query: 721  NSKLIYLYDEYGIPYDSMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSALLRRHPEP 780
            +SK+++L+DEYGIPYDSMF E+KDSNFLVEEFMLLANRT AEVI R FPDSALLRRHPEP
Sbjct: 721  SSKIVFLFDEYGIPYDSMFSERKDSNFLVEEFMLLANRTAAEVICRAFPDSALLRRHPEP 780

Query: 781  MLRKLREFETFCSKHGFELDTSSSVHFQQSLEQIRIELQDDPLLFDILISYATRPMQLAT 840
             +RKLREFE FC KHG ELDT+SS HF +SLE+IR +L+DD +L+ IL+SYA+RPMQLAT
Sbjct: 781  NMRKLREFEAFCCKHGLELDTTSSGHFHRSLERIREKLKDDSMLYGILMSYASRPMQLAT 840

Query: 841  YFCSGELKDGETR-SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKMYLKHKGVI 900
            YFCSG +KD      HY LAVPLYTHFTSPLRRYPDIVVHRTLAAA+EAE++Y++ + ++
Sbjct: 841  YFCSGVMKDNTNDWGHYGLAVPLYTHFTSPLRRYPDIVVHRTLAAAVEAEELYMRSRRIL 900

Query: 901  QKVNSNEE-TRCFTGIYFDKDAADSLEGREALSSAALKHGVPCSKLLLDVALHCNDRKLA 960
              V+  EE TRCFTGIYFDK+AA+SLEGREA S+AALKH VPC++LL DVA +CN+RKLA
Sbjct: 901  HNVSMKEEVTRCFTGIYFDKNAAESLEGREAFSAAALKHKVPCTELLSDVAAYCNERKLA 960

Query: 961  SKHVADGIEKLYMWALLKKKKILFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLAV 1020
            S+HV D  EKLYMW LLKKK++LFS+ARVLGLGPRFMS+Y+QKLAIERRIYYDEVEGL V
Sbjct: 961  SRHVKDACEKLYMWVLLKKKEVLFSEARVLGLGPRFMSIYVQKLAIERRIYYDEVEGLTV 1020

Query: 1021 EWLETTSTLVLRFFCSRRSHRSRGSVKWKALEDVALVISPCDQNVKERTLGVSSNGGASK 1080
            EW E TSTLVL     +R+ R  G   +KAL +VA +++PC+  V    +     G A +
Sbjct: 1021 EWFEATSTLVLSLCAFKRTVRKAGPGYYKALNEVAWLVNPCNLKVDPAAV----EGSAIQ 1080

Query: 1081 GGSAVVEQDSNLKSHV---SDTGVDPAIFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLY 1124
              +  ++ +     HV   S++ +DP +FPLTVRLLSTIPVALHA+GG+DGP DIGVRLY
Sbjct: 1081 CSNTQLDDNDMPSQHVDPISESEIDPLVFPLTVRLLSTIPVALHAIGGNDGPTDIGVRLY 1116

BLAST of Cucsa.365270 vs. TrEMBL
Match: M5XPD1_PRUPE (DIS3-like exonuclease 2 OS=Prunus persica GN=PRUPE_ppa015523mg PE=3 SV=1)

HSP 1 Score: 1217.2 bits (3148), Expect = 0.0e+00
Identity = 630/1089 (57.85%), Postives = 792/1089 (72.73%), Query Frame = 1

Query: 40   ASYTSVNGILGEASECMENGRIDANLTSPSNYSSLTQQENHSNQQIEHGLTRGDKIGFSS 99
            A+  S N I  E SEC+ NGR   ++T+P     L Q +   +   EHG+ +     FSS
Sbjct: 2    AASNSANEIRSEVSECLGNGRTADHVTTP-----LKQHQFVLHPPYEHGMIKASNFPFSS 61

Query: 100  LPPLHINEQAELSASHNLMNQNHHSSDAGGRV-TKSCPEQIASGRYSGISLNQHSPPADV 159
            LP +HINEQ       +L NQ+    D G RV   SCPE +A G   GI + +  P    
Sbjct: 62   LPTMHINEQENPEDVQSLENQHSLPCDPGQRVCANSCPESVACGESPGIFILKDFPHH-- 121

Query: 160  TDNNTQRKYFPSHWSVDDVNEGLQKGGIFKALFRVNAHNRLEAYCKIDGLPIDVLINGIA 219
             +    RKYF  HWS++  N+ ++KG  FKALFRVNAHNR EAYCK+DG+P DVLI G+A
Sbjct: 122  IERYAPRKYFTQHWSMEATNDAIEKGEAFKALFRVNAHNRHEAYCKVDGVPTDVLIGGLA 181

Query: 220  SQNRAVEGDIVAIKLDPFTSWTKMKGTSEAHNNMHSMEDANLPAELTEKNDHNCKGKNKV 279
             QNRAVEGDIV +K+DP   WT+MKG++    +   ++D NL  E      +NCKGK KV
Sbjct: 182  EQNRAVEGDIVVVKVDPLPLWTRMKGSAGTCTSSAPLDDFNLQLENNVVAGYNCKGKAKV 241

Query: 280  DADVKSDSFRSTSLPDKRCCSEDKVLDGVACDVLLSNYEQCDINELSVVNPSQAHHSSNQ 339
            D      + RS+ LP++    E+ V +      +  +          + + S    S  Q
Sbjct: 242  DEVYLYGNDRSSLLPERGSRPEESVGESFHSGPIGQSSYDHVAGRYPLPSDSIQAGSPEQ 301

Query: 340  DDVSKAIGRICALINLYPAKRPTGRVVTILEKSRLRENVVGHLNVKKFLSFQEFYVKEST 399
            ++V  ++ R+CA+IN +P+KRPTGRVV I+E+S  R+ +VG LNVK+++S++EF  K+  
Sbjct: 302  NEVRLSVERLCAMINSFPSKRPTGRVVAIVERSPRRDAIVGFLNVKQWISYREFCRKDMR 361

Query: 400  KSCLSPSQNCGYVQLMPNDARFPIMMVLAGDLPNCIKKRLDNGDVTVENELVAARIYEWV 459
            K+  S   N  Y+Q+ P D RFP M+VL  +LP+ IKKRL++GD T+E EL AARI EW 
Sbjct: 362  KNKNSSFSNHEYIQMTPIDPRFPKMVVLVRNLPDSIKKRLEDGDETIEMELFAARIDEWD 421

Query: 460  KESSSPRAHVLHVLGRGNEVESHIDAILFENAFRTCEFSQDSLSCVPQTPWKIPPEELQC 519
            +ESS+P+A +L+  GRG E++  I+AILF+NA  + EFS +SLSC+P  PW++P EE Q 
Sbjct: 422  EESSAPQAVILNAFGRGCELQPQIEAILFQNAINSSEFSPESLSCLPHLPWEVPQEEFQT 481

Query: 520  RRDIRNLCIFTIDPSSASDLDDALSVQRLANGIFRVGIHIADVSYFVLPDTALDKEAQIR 579
            RRD+RNLCIFTIDPS+A+DLDDALSV +L+NGI+RVGIHIADVS+FVLP T LD+EAQ R
Sbjct: 482  RRDLRNLCIFTIDPSTATDLDDALSVDKLSNGIYRVGIHIADVSHFVLPGTPLDEEAQSR 541

Query: 580  STSVYLLQRKIPMLPPLLSESIGSLNPGVDRLAFSLFLDINSCGDVKDFWIERTVICCCC 639
            STSVY+ +RK+PMLPPLLSE++GSLNPGV+RLAFS+FLD+N  GDV D WI RTVI  CC
Sbjct: 542  STSVYMSRRKLPMLPPLLSENVGSLNPGVERLAFSIFLDMNHAGDVVDRWIGRTVIRSCC 601

Query: 640  KLSYEHAQDIIDGLIDSDSSELFGNNCPQLHGQFTWHDVISSVKLLHEISKTVKEKRFRN 699
            KLSYEH QDIIDG  + +S ++ GN  PQLHG F W DV+ SVK LHEIS+ +KE+RF +
Sbjct: 602  KLSYEHTQDIIDGKFNLESVDILGNGRPQLHGHFEWFDVLRSVKDLHEISRILKERRFSD 661

Query: 700  GALRLENSKLIYLYDEYGIPYDSMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSALL 759
            GAL+LE+SK++ L+DEYG+PYDS+  E K+SNFLVEEFMLLANRT AEVISR FPDSALL
Sbjct: 662  GALQLESSKVVILFDEYGVPYDSIHSELKESNFLVEEFMLLANRTAAEVISRAFPDSALL 721

Query: 760  RRHPEPMLRKLREFETFCSKHGFELDTSSSVHFQQSLEQIRIELQDDPLLFDILISYATR 819
            RRHPEP LRKLREFE FCSKHG ELDTSSS  FQ SLE+IR EL+DD +LF+IL++YAT+
Sbjct: 722  RRHPEPNLRKLREFEAFCSKHGLELDTSSSGQFQLSLEKIREELKDDCVLFNILMNYATK 781

Query: 820  PMQLATYFCSGELKDGETR-SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKMYL 879
            PMQLA YFCSGELKD E    HY LAVPLYTHFTSPLRRYPDI+VHR L+AAIEAE++ L
Sbjct: 782  PMQLAAYFCSGELKDRENDWGHYGLAVPLYTHFTSPLRRYPDILVHRMLSAAIEAEELLL 841

Query: 880  KHKGVIQKVNSNEETR--CFTGIYFDKDAADSLEGREALSSAALKHGVPCSKLLLDVALH 939
            KH+ ++   N  +E R  CFTGIYFDKDAA+S E REALS+A++KHG+PCS+LL DVA +
Sbjct: 842  KHRRMLNNFNRGDECRMKCFTGIYFDKDAAESYESREALSAASMKHGIPCSELLTDVAAY 901

Query: 940  CNDRKLASKHVADGIEKLYMWALLKKKKILFSDARVLGLGPRFMSVYIQKLAIERRIYYD 999
            CN+RKLAS+HV D  +KLYMWALLKKK+IL S+ARV+GLGPRFMS+YI KLA+ERRIYYD
Sbjct: 902  CNERKLASRHVKDACDKLYMWALLKKKEILLSEARVMGLGPRFMSIYIYKLAVERRIYYD 961

Query: 1000 EVEGLAVEWLETTSTLVLRFFCSRRSHRSRGSVKWKALEDVALVISPCDQNVKERTLGVS 1059
            EVEG+  EWL+ TSTLVL    +RRS R     K +ALEDVALV  P D   +   +G S
Sbjct: 962  EVEGMMGEWLDATSTLVLTLCSNRRSLRRGSPGKCRALEDVALVARPYDLKAELGAVGNS 1021

Query: 1060 SNGGASKGGSAVVEQDSNLKSHVSDTGVDPAIFPLTVRLLSTIPVALHAVGGDDGPIDIG 1119
            +N GA+     V    SN      ++ +DP +FPLT+R+LSTIPV LHA+GGDDGPIDIG
Sbjct: 1022 TNEGAAAQDVGVATHSSN------ESEIDPLVFPLTLRVLSTIPVVLHAIGGDDGPIDIG 1077

Query: 1120 VRLYMSSYL 1125
             RLYMSSYL
Sbjct: 1082 ARLYMSSYL 1077

BLAST of Cucsa.365270 vs. TrEMBL
Match: W9QVR2_9ROSA (DIS3-like exonuclease 2 OS=Morus notabilis GN=L484_020163 PE=3 SV=1)

HSP 1 Score: 1214.9 bits (3142), Expect = 0.0e+00
Identity = 651/1129 (57.66%), Postives = 808/1129 (71.57%), Query Frame = 1

Query: 4    AVEQSTPERNGDG-DKEKKKKRRSNRRSKHNPSLTTSASYTSVNGILGEASECMENGRID 63
            +VEQ   E+  DG DKEKKKKRRS RRSK N S        SVN   GEA+E   NGR  
Sbjct: 5    SVEQFVVEKIEDGADKEKKKKRRSGRRSKQNAS-------QSVNESRGEAAEYSGNGRST 64

Query: 64   ANLTSPSNYSSLTQQENHSNQQIEHGLTRGDKIGFSSLPPLHINEQAELSASHNLMNQNH 123
             +L + S      Q +       +HG T    + F+SLPPLHINEQ       N  NQN 
Sbjct: 65   NHLLAYSR-----QPKFGICPPDDHGFTNTSNVAFNSLPPLHINEQTNSEDMQNSQNQNP 124

Query: 124  HSSDAGGRVTKSCPEQIASGRYSGISLNQHSPPADVTDNNTQRKYFPSHWSVDDVNEGLQ 183
            H S+ GG +      + ++ R S         P+ + ++  Q+K +  HWS++ VN+ L+
Sbjct: 125  HPSNLGGEMFSKSYSEPSTYRGSPDLFMTKVFPSHLIESYAQKKLYALHWSLEAVNDALE 184

Query: 184  KGGIFKALFRVNAHNRLEAYCKIDGLPIDVLINGIASQNRAVEGDIVAIKLDPFTSWTKM 243
            KG +FKALFRVNAHNRLEAYCKIDG+P+DV I+G+A+QNRAVEGDIVA+K+DP   WT+M
Sbjct: 185  KGDVFKALFRVNAHNRLEAYCKIDGVPVDVFISGVAAQNRAVEGDIVAVKVDPLPLWTRM 244

Query: 244  KGTSEAHNNMHSMEDANLPAELTEKNDHNCKGKNKVDADVKSDSFRSTSLPDKRCCSEDK 303
            KG++   +N   +ED NL +E  E   ++CKGK+KVD D +  +       +K   SE  
Sbjct: 245  KGSTAGGSNSAPVEDLNLLSECNEMAGNSCKGKSKVDVDYQYANHGCCLPAEKEFHSERN 304

Query: 304  V-LDGVACDVLL--SNYEQCDINELSVVNPSQAHHSSNQDDVSKAIGRICALINLYPAKR 363
              LD       +  S+ E  D         S    SS  + V  AIGR+CA+I+ +P+KR
Sbjct: 305  TSLDEPVQPESIGPSSCENMDGYHFPASGTSHVGSSSGMNHVRDAIGRMCAMISSFPSKR 364

Query: 364  PTGRVVTILEKSRLRENVVGHLNVKKFLSFQEFYVKESTKSCLSPS-QNCGYVQLMPNDA 423
            PTGRV+ ++EKS  R+ VVG LNVK+++ +QE   K++ K+  + +  +  Y+QL P D 
Sbjct: 365  PTGRVLAVIEKSPRRKAVVGFLNVKQWILYQEVCRKDAKKNKSTLAFTDYEYIQLTPIDP 424

Query: 424  RFPIMMVLAGDLPNCIKKRLDNGDVTVENELVAARIYEWVKESSSPRAHVLHVLGRGNEV 483
            R P MMVL   LP+CIKKRL+NGDVT+E ELVAA+I  W +ES  P+A V H  G+G E+
Sbjct: 425  RLPKMMVLVQGLPDCIKKRLENGDVTLEIELVAAKIDNWGEESPFPQACVSHTFGQGGEL 484

Query: 484  ESHIDAILFENAFRTCEFSQDSLSCVPQTPWKIPPEELQCRRDIRNLCIFTIDPSSASDL 543
             S + AILFENA  + +FS  S SC+P  PW++P EELQ RRD+R LCIFTIDPS+A++L
Sbjct: 485  NSQLGAILFENAICSADFSPKSFSCLPNVPWEVPLEELQSRRDLRKLCIFTIDPSTATEL 544

Query: 544  DDALSVQRLANGIFRVGIHIADVSYFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLSE 603
            DDALS++RL+N  FRVGIHIADVSYFVLPDT LDKEAQ+RSTSVY+ ++K+ MLPPLLSE
Sbjct: 545  DDALSIERLSNRDFRVGIHIADVSYFVLPDTELDKEAQMRSTSVYMSRKKLSMLPPLLSE 604

Query: 604  SIGSLNPGVDRLAFSLFLDINSCGDVKDFWIERTVICCCCKLSYEHAQDIIDGLIDSDSS 663
            +IGSLN GVDRLAFS+FLDIN  GDV+D WI RTVI  CCKLSYEHAQ+IIDG +D+ S 
Sbjct: 605  NIGSLNAGVDRLAFSMFLDINLAGDVEDRWIGRTVIKSCCKLSYEHAQEIIDGPMDTGS- 664

Query: 664  ELF-GNNCPQLHGQFTWHDVISSVKLLHEISKTVKEKRFRNGALRLENSKLIYLYDEYGI 723
             LF GNNCPQLHG F W DV++SVK LHE+SK ++ KRF NGAL LE+ K+++ YDE G 
Sbjct: 665  -LFSGNNCPQLHGHFEWVDVVNSVKDLHELSKILRGKRFSNGALALESLKVVFRYDECGN 724

Query: 724  PYDSMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSALLRRHPEPMLRKLREFETFCS 783
            PYDSM  E+K SNFLVEEFMLLANRT AEVISR FPD ALLRRHPEP +RKLREFE FC 
Sbjct: 725  PYDSMLSERKASNFLVEEFMLLANRTAAEVISRAFPDCALLRRHPEPNMRKLREFEAFCH 784

Query: 784  KHGFELDTSSSVHFQQSLEQIRIELQDDPLLFDILISYATRPMQLATYFCSGELKDGETR 843
            KHG ELDTSSS  F  SL++I  +L+DD  LFDI+++YA RPMQLATYFC+G+LKD E  
Sbjct: 785  KHGLELDTSSSRQFHLSLQRIGEKLKDDSTLFDIIMNYAARPMQLATYFCTGDLKDDEND 844

Query: 844  -SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKMYLKHKGVIQKVNSNEET--RC 903
              HYALAVPLYTHFTSPLRRYPDIVVHRTLAA IEAE++YLKH+    K +  +E   +C
Sbjct: 845  WGHYALAVPLYTHFTSPLRRYPDIVVHRTLAAIIEAEELYLKHEKTFNKFHRGQEATRKC 904

Query: 904  FTGIYFDKDAADSLEGREALSSAALKHGVPCSKLLLDVALHCNDRKLASKHVADGIEKLY 963
            FTGI F+KDAA+S EGREALS+AA  H +P ++LL  VA +CNDRKLAS+HV D  +KL+
Sbjct: 905  FTGINFEKDAAESREGREALSAAARNHRIPGTELLAKVAAYCNDRKLASRHVKDACDKLH 964

Query: 964  MWALLKKKKILFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLETTSTLVLR 1023
            MWALLKKK++L S+ARVLGLGPRFMS+YIQKLAIERRIYYDEVEGL  EWLE TSTLVL 
Sbjct: 965  MWALLKKKQVLLSEARVLGLGPRFMSIYIQKLAIERRIYYDEVEGLMPEWLEATSTLVLN 1024

Query: 1024 FFCSRRSHRSRGSVKWKALEDVALVISPCDQNVKERTLGVSSNGGASKGGSAVVEQDSNL 1083
             + +R   R     KW+ +EDVAL++SPCD   +   +G SS   +   GS+VV   S  
Sbjct: 1025 LYPNRLCTRRGSPGKWRPIEDVALIVSPCDLQAEPGVVGSSS---SEPVGSSVVTSQSG- 1084

Query: 1084 KSHVSDTGVDPAIFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSY 1124
                S+T +DP++FP+TVRLLSTIPVA+HA+GGDDGP+DIG RLYMSSY
Sbjct: 1085 ---SSETELDPSVFPITVRLLSTIPVAVHAIGGDDGPVDIGARLYMSSY 1112

BLAST of Cucsa.365270 vs. TAIR10
Match: AT1G77680.1 (AT1G77680.1 Ribonuclease II/R family protein)

HSP 1 Score: 979.5 bits (2531), Expect = 1.7e-285
Identity = 551/1131 (48.72%), Postives = 738/1131 (65.25%), Query Frame = 1

Query: 1    MRAAVEQSTPERNGDGDKEKKKK-RRSNRRSKHNPSLTTSASYTSVNGILGEASECMENG 60
            M++A  + + ER  +G K+K+ + ++ NRRSK +      A             E ++ G
Sbjct: 1    MKSASSEQSVERIENGHKKKRNRPQKQNRRSKQSSVPIEDA----------HVEESLD-G 60

Query: 61   RIDANLTSPSNYSSLTQQENHSNQQIEHGLTRGDKIGFSSLPPLHINEQAELSASHNLMN 120
            R D++ +   + +S ++Q+  +  ++E    R   + F+S+PP+         ++  L++
Sbjct: 61   R-DSSRSKAKDSTSSSKQQRPNTDELE--AMRASNVAFNSMPPMRAESGYPRRSASPLLS 120

Query: 121  QNHHSSDAGGRVTKSCPEQIASGRYSGISLNQHSPPADVTDNNTQRKYFPSHWSVDDVNE 180
                S      ++KSCP+  A  +  G++           + ++QRK F SHWS+D V E
Sbjct: 121  SPEVSKQL---LSKSCPDPRACEQSPGMNGELFQQ----IEGSSQRKIFSSHWSLDAVTE 180

Query: 181  GLQKGGIFKALFRVNAHNRLEAYCKIDGLPIDVLINGIASQNRAVEGDIVAIKLDPFTSW 240
             L+KG  FKALFRVNAHNR EAYCKIDG+P D+LING   Q+RAVEGD V IKLDP + W
Sbjct: 181  ALEKGEAFKALFRVNAHNRNEAYCKIDGVPTDILINGNVCQSRAVEGDTVVIKLDPLSLW 240

Query: 241  TKMKGTSEAHNNMHSMEDANLPAELTEKNDHNCKGKNKVDADVKSDSFRSTSLPDKRCCS 300
             KMKG      +    E  N P    EK+D   + KN +D     + F      +K    
Sbjct: 241  PKMKGF--VTESAAKPEGTNSPP---EKDDKKARQKNGIDV---VEGFEDGFSKNKSSVI 300

Query: 301  EDKVLDGV------ACDVLLSNYEQCDINELSVVNPSQAHHSSNQDDVSKAIGRICALIN 360
                 +GV      + D  L ++  C+                 Q     A+ ++C +++
Sbjct: 301  GKGAKNGVTPSSPPSLDSCLGSF--CE-----------------QKGNCSAVDKLCGILS 360

Query: 361  LYPAKRPTGRVVTILEKSRLRENVVGHLNVKKFLSFQEFYVKESTKSCLSPSQNCGYVQL 420
             +P KRPTG+VV ++EKS +R+++VG L+VK ++ ++E   K   KS LS S +  YVQL
Sbjct: 361  SFPHKRPTGQVVAVVEKSLVRDSIVGLLDVKGWIHYKESDPKRC-KSPLSLSDD-EYVQL 420

Query: 421  MPNDARFPIMMVLAGDLPNCIKKRLDNGDVTVENELVAARIYEWVKESSSPRAHVLHVLG 480
            MP D RFP ++V    LP  I+ RL+N D  +E ELVAA+I +W + S  P A + H+ G
Sbjct: 421  MPADPRFPKLIVPFHVLPGSIRARLENLDPNLEAELVAAQIVDWGEGSPFPVAQITHLFG 480

Query: 481  RGNEVESHIDAILFENAFRTCEFSQDSLSCVPQTPWKIPPEELQCRRDIRNLCIFTIDPS 540
            RG+E+E  I+AIL++N+    +FS  SL+ +P+ PW++P EE+Q R+D+R+LC+ TIDPS
Sbjct: 481  RGSELEPQINAILYQNSVCDSDFSPGSLTSLPRVPWEVPEEEVQRRKDLRDLCVLTIDPS 540

Query: 541  SASDLDDALSVQRLANGIFRVGIHIADVSYFVLPDTALDKEAQIRSTSVYLLQRKIPMLP 600
            +A+DLDDALSVQ L  G FRVG+HIADVSYFVLP+TALD EA+ RSTSVYL+QRKI MLP
Sbjct: 541  TATDLDDALSVQSLPGGFFRVGVHIADVSYFVLPETALDTEARFRSTSVYLMQRKISMLP 600

Query: 601  PLLSESIGSLNPGVDRLAFSLFLDINSCGDVKDFWIERTVICCCCKLSYEHAQDIIDGLI 660
            PLLSE++GSL+PG DRLAFS+  D+N  GDV D WI RT+I  CCKLSY+HAQDIIDG  
Sbjct: 601  PLLSENVGSLSPGADRLAFSILWDLNREGDVIDRWIGRTIIRSCCKLSYDHAQDIIDG-- 660

Query: 661  DSDSSELFGNNCPQLHGQFTWHDVISSVKLLHEISKTVKEKRFRNGALRLENSKLIYLYD 720
                S++  N  P LHG F W DV  SVK L EIS T+++KRFRNGAL+LENSK ++L+D
Sbjct: 661  ---KSDVAENGWPALHGSFKWCDVTRSVKQLSEISTTLRQKRFRNGALQLENSKPVFLFD 720

Query: 721  EYGIPYDSMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSALLRRHPEPMLRKLREFE 780
            E+G+PYD +   +K SNFLVEEFMLLAN T AEVIS+ +  S+LLRRHPEP  RKL+EFE
Sbjct: 721  EHGVPYDFVTCSRKGSNFLVEEFMLLANMTAAEVISQAYRASSLLRRHPEPNTRKLKEFE 780

Query: 781  TFCSKHGFELDTSSSVHFQQSLEQIRIELQDDPLLFDILISYATRPMQLATYFCSGELKD 840
             FCSKHG +LD SSS   Q SLE+I   L+DD +  DIL +YA +PMQLA+YFC+G LKD
Sbjct: 781  GFCSKHGMDLDISSSGQLQDSLEKITGNLKDDSVFVDILNNYAIKPMQLASYFCTGNLKD 840

Query: 841  GETR-SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKMYLKHKGVIQKVNSNEET 900
                  HYALAVPLYTHFTSPLRRYPDIVVHR LAAA+EAE++Y K K    +   +E  
Sbjct: 841  SVAEWGHYALAVPLYTHFTSPLRRYPDIVVHRALAAALEAEELYSKQK----QTAIDEGR 900

Query: 901  RCFTGIYFDKDAADSLEGREALSSAALKHGVPCSKLLLDVALHCNDRKLASKHVADGIEK 960
             CFTGI+F+KDAA+S+EG+EALS AALKHGVP +++L DVA +CN+RKLA++ V D  +K
Sbjct: 901  SCFTGIHFNKDAAESIEGKEALSVAALKHGVPSTEILSDVAAYCNERKLAARKVRDACDK 960

Query: 961  LYMWALLKKKKILFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLETTSTLV 1020
            LY W +LK+K+I   +ARV+ LG RFM+VYI KL IERRIYYD++EGL  +WLE TSTL+
Sbjct: 961  LYTWFVLKQKEIFPCEARVMNLGSRFMTVYISKLGIERRIYYDQIEGLCADWLEATSTLI 1020

Query: 1021 LRFFCSRRSHRSRGSVKWKALEDVALVISPCDQNVKERTLGVSSNGGASKGGSAVVEQDS 1080
            +    S+R  R      +K +++   ++SPC+  V +               SA+   D+
Sbjct: 1021 VDKLYSKRGGRG----FFKPMKEAVYLVSPCEVCVAK--------------CSALSVHDT 1054

Query: 1081 NLKSHVSDTGVDPAIFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSY 1124
                 VS   V PA+FPLT++L STIPV LHAVGGDDGP+DIG RLYMSSY
Sbjct: 1081 ESPEAVSIDEVAPAVFPLTIQLFSTIPVVLHAVGGDDGPLDIGARLYMSSY 1054

BLAST of Cucsa.365270 vs. TAIR10
Match: AT2G17510.2 (AT2G17510.2 ribonuclease II family protein)

HSP 1 Score: 243.0 bits (619), Expect = 8.6e-64
Identity = 172/554 (31.05%), Postives = 264/554 (47.65%), Query Frame = 1

Query: 359 RPTGRVVTILEKSRLRENVVGHLNVKKFLSFQEFYVKESTKSCLSPSQNCGYVQLMPNDA 418
           RP+GRVV ++ ++                 +  +       S  + S    +   +  D 
Sbjct: 381 RPSGRVVGVIRRN-----------------WHSYCGSLEPMSLPAGSGGTAHALFVSKDR 440

Query: 419 RFPIMMVLAGDLPNCIKKRLDNGDVTVENELVAARIYEWVKESSSPRAHVLHVLGR---- 478
           R P + +    L N +  R            +   +  W ++S  P  H +  +G+    
Sbjct: 441 RIPKIRINTRQLQNLLDMR------------IVVAVDSWDRQSRYPSGHYVRPIGKIGDK 500

Query: 479 --GNEVESHID----------------AILFENAFRTCEFSQDSLSCVPQTPWKIPPEEL 538
               EV  HI+                 +L EN      FS   L+C+P  PW +  E++
Sbjct: 501 ETETEVRDHINLFDSILVGVRWARVGKVVLIENDVDYSPFSSQVLACLPPLPWSVSSEDV 560

Query: 539 Q--CRRDIRNLCIFTIDPSSASDLDDALSVQRLANGIFRVGI------------HIADVS 598
               R+D+R+L +F++DP    D+DDAL    L NG F +G+            +IADV+
Sbjct: 561 SNPVRQDLRHLLVFSVDPPGCKDIDDALHCTSLPNGNFELGVRILESSDSHKYDYIADVT 620

Query: 599 YFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLSESIGSLNPGVDRLAFSLFLDINSCG 658
            FV P T LD EA  R TSVYL++R+I MLP  L+E I SL   V+RLAFS+  +++   
Sbjct: 621 NFVHPGTPLDDEASKRGTSVYLVERRIDMLPKPLTEDICSLRADVERLAFSVIWEMSPDA 680

Query: 659 DVKDFWIERTVICCCCKLSYEHAQDIIDGLIDSDSSELFGNNCPQLHGQFTWHDVISSVK 718
           ++      +++I     LSY  AQ  +D    +DS                   + + ++
Sbjct: 681 EIISTRFTKSIIKSSAALSYIEAQARMDDSRLTDS-------------------LTTDLR 740

Query: 719 LLHEISKTVKEKRFRNGALRLENSKLIYLYD-EYGIPYDSMFYEQKDSNFLVEEFMLLAN 778
            ++ ++K ++++R   GAL L ++++ +  D E   P +   Y+  ++N +VEEFML AN
Sbjct: 741 NMNTLAKIMRQRRIDRGALTLASAEVKFDIDPENHDPLNIGMYQILEANQMVEEFMLAAN 800

Query: 779 RTVAEVISRTFPDSALLRRHPEPMLRKLREFETFCSKHGFELDTSSSVHFQQSLEQIRIE 838
            +VA  I + FP  +LLRRHP P    L       +  G  LD SSS     SL++    
Sbjct: 801 VSVAGQILKLFPSCSLLRRHPTPTREMLEPLLRTAAAIGLTLDVSSSKALADSLDR---A 860

Query: 839 LQDDPLLFDILISYATRPMQLATYFCSGELKDGETRSHYALAVPLYTHFTSPLRRYPDIV 876
           + +DP    ++   ATR M  A YFCSG+L   E   HY LA PLYTHFTSP+RRY D+ 
Sbjct: 861 VGEDPYFNKLIRILATRCMTQAVYFCSGDLSPPEYH-HYGLAAPLYTHFTSPIRRYADVF 882

HSP 2 Score: 55.5 bits (132), Expect = 2.5e-07
Identity = 35/125 (28.00%), Postives = 57/125 (45.60%), Query Frame = 1

Query: 156 ADVTDNNTQRKYFPSHWSVDDVNEGLQKGGIFKALFRVNAHNRLEAYCKIDGLPIDVLIN 215
           AD +  + ++  +  H  + ++  GL +G   +   RVN  N  EAY   + +  +++I 
Sbjct: 249 ADDSRPSKRKLIYQEHKPMSEITAGLHRGIYHQGKLRVNRFNPYEAYVGSESIGEEIIIY 308

Query: 216 GIASQNRAVEGDIVAIKLDPFTSWTKMKGTS------EAHNNMHSMEDANLPAELTEKND 275
           G ++ NRA +GDIVA++L P   W   K  S      E  + +H   D    A  T    
Sbjct: 309 GRSNMNRAFDGDIVAVELLPRDQWQDEKALSIAEEDDEEDDTVHLAPDNVDDAPRTSNLS 368

BLAST of Cucsa.365270 vs. TAIR10
Match: AT5G02250.1 (AT5G02250.1 Ribonuclease II/R family protein)

HSP 1 Score: 82.4 bits (202), Expect = 1.9e-15
Identity = 45/131 (34.35%), Postives = 77/131 (58.78%), Query Frame = 1

Query: 519 RRDIRNLCIFTIDPSSASDLDDALSVQRLANGIFRVGIHIADVSYFVLPDTALDKEAQIR 578
           R D+ +L ++ ID   A +LDDALS  RL +G  ++ IH+AD + +V P + +D+EA+ R
Sbjct: 399 RIDLTHLKVYAIDVDEADELDDALSATRLQDGRIKIWIHVADPARYVTPGSKVDREARRR 458

Query: 579 STSVYLLQRKIPMLPPLLSESIGSLNPGVDRLAFSLFLDINSCGDVKDFWIERTVICCCC 638
            TSV+L     PM P  L+    SL  G +  A S+ + + S G + ++ ++ ++I    
Sbjct: 459 GTSVFLPTATYPMFPEKLAMEGMSLRQGENCNAVSVSVVLRSDGCITEYSVDNSIIRPTY 518

Query: 639 KLSYEHAQDII 650
            L+YE A +++
Sbjct: 519 MLTYESASELL 529

HSP 2 Score: 35.0 bits (79), Expect = 3.5e-01
Identity = 14/30 (46.67%), Postives = 18/30 (60.00%), Query Frame = 1

Query: 839 HYALAVPLYTHFTSPLRRYPDIVVHRTLAA 869
           H  L +P Y  FTSP+RRY D+  H  + A
Sbjct: 662 HGVLGIPGYVQFTSPIRRYMDLTAHYQIKA 691

BLAST of Cucsa.365270 vs. NCBI nr
Match: gi|449446430|ref|XP_004140974.1| (PREDICTED: DIS3-like exonuclease 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 2260.3 bits (5856), Expect = 0.0e+00
Identity = 1124/1125 (99.91%), Postives = 1124/1125 (99.91%), Query Frame = 1

Query: 1    MRAAVEQSTPERNGDGDKEKKKKRRSNRRSKHNPSLTTSASYTSVNGILGEASECMENGR 60
            MRAAVEQSTPERNGDGDKEKKKKRRSNRRSKHNPSLTTSASYTSVNGILGEASECMENGR
Sbjct: 1    MRAAVEQSTPERNGDGDKEKKKKRRSNRRSKHNPSLTTSASYTSVNGILGEASECMENGR 60

Query: 61   IDANLTSPSNYSSLTQQENHSNQQIEHGLTRGDKIGFSSLPPLHINEQAELSASHNLMNQ 120
            IDANLTSPSNYSSLTQQENHSNQQIEHGLTRGDKIGFSSLPPLHINEQAELSASHNLMNQ
Sbjct: 61   IDANLTSPSNYSSLTQQENHSNQQIEHGLTRGDKIGFSSLPPLHINEQAELSASHNLMNQ 120

Query: 121  NHHSSDAGGRVTKSCPEQIASGRYSGISLNQHSPPADVTDNNTQRKYFPSHWSVDDVNEG 180
            NHHSSDAGGRVTKSCPEQIASGRYSGISLNQHSPPADVTDNNTQRKYFPSHWSVDDVNEG
Sbjct: 121  NHHSSDAGGRVTKSCPEQIASGRYSGISLNQHSPPADVTDNNTQRKYFPSHWSVDDVNEG 180

Query: 181  LQKGGIFKALFRVNAHNRLEAYCKIDGLPIDVLINGIASQNRAVEGDIVAIKLDPFTSWT 240
            LQKGGIFKALFRVNAHNRLEAYCKIDGLPIDVLINGIASQNRAVEGDIVAIKLDPFTSWT
Sbjct: 181  LQKGGIFKALFRVNAHNRLEAYCKIDGLPIDVLINGIASQNRAVEGDIVAIKLDPFTSWT 240

Query: 241  KMKGTSEAHNNMHSMEDANLPAELTEKNDHNCKGKNKVDADVKSDSFRSTSLPDKRCCSE 300
            KMKGTSEAHNNMHSMEDANLPAELTEKNDHNCKGKNKVDADVKSDSFRSTSLPDKRCCSE
Sbjct: 241  KMKGTSEAHNNMHSMEDANLPAELTEKNDHNCKGKNKVDADVKSDSFRSTSLPDKRCCSE 300

Query: 301  DKVLDGVACDVLLSNYEQCDINELSVVNPSQAHHSSNQDDVSKAIGRICALINLYPAKRP 360
            DKVLDGVACDVLLSNYEQCDINELSVVNPSQAHHSSNQDDVSKAIGRICALINLYPAKRP
Sbjct: 301  DKVLDGVACDVLLSNYEQCDINELSVVNPSQAHHSSNQDDVSKAIGRICALINLYPAKRP 360

Query: 361  TGRVVTILEKSRLRENVVGHLNVKKFLSFQEFYVKESTKSCLSPSQNCGYVQLMPNDARF 420
            TGRVVTILEKSRLRENVVGHLNVKKFLSFQEFYVKESTKSCLSPSQNCGYVQLMPNDARF
Sbjct: 361  TGRVVTILEKSRLRENVVGHLNVKKFLSFQEFYVKESTKSCLSPSQNCGYVQLMPNDARF 420

Query: 421  PIMMVLAGDLPNCIKKRLDNGDVTVENELVAARIYEWVKESSSPRAHVLHVLGRGNEVES 480
            PIMMVLAGDLPNCIKKRLDNGDVTVENELVAARIYEWVKESSSPRAHVLHVLGRGNEVES
Sbjct: 421  PIMMVLAGDLPNCIKKRLDNGDVTVENELVAARIYEWVKESSSPRAHVLHVLGRGNEVES 480

Query: 481  HIDAILFENAFRTCEFSQDSLSCVPQTPWKIPPEELQCRRDIRNLCIFTIDPSSASDLDD 540
            HIDAILFENA RTCEFSQDSLSCVPQTPWKIPPEELQCRRDIRNLCIFTIDPSSASDLDD
Sbjct: 481  HIDAILFENAIRTCEFSQDSLSCVPQTPWKIPPEELQCRRDIRNLCIFTIDPSSASDLDD 540

Query: 541  ALSVQRLANGIFRVGIHIADVSYFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLSESI 600
            ALSVQRLANGIFRVGIHIADVSYFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLSESI
Sbjct: 541  ALSVQRLANGIFRVGIHIADVSYFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLSESI 600

Query: 601  GSLNPGVDRLAFSLFLDINSCGDVKDFWIERTVICCCCKLSYEHAQDIIDGLIDSDSSEL 660
            GSLNPGVDRLAFSLFLDINSCGDVKDFWIERTVICCCCKLSYEHAQDIIDGLIDSDSSEL
Sbjct: 601  GSLNPGVDRLAFSLFLDINSCGDVKDFWIERTVICCCCKLSYEHAQDIIDGLIDSDSSEL 660

Query: 661  FGNNCPQLHGQFTWHDVISSVKLLHEISKTVKEKRFRNGALRLENSKLIYLYDEYGIPYD 720
            FGNNCPQLHGQFTWHDVISSVKLLHEISKTVKEKRFRNGALRLENSKLIYLYDEYGIPYD
Sbjct: 661  FGNNCPQLHGQFTWHDVISSVKLLHEISKTVKEKRFRNGALRLENSKLIYLYDEYGIPYD 720

Query: 721  SMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSALLRRHPEPMLRKLREFETFCSKHG 780
            SMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSALLRRHPEPMLRKLREFETFCSKHG
Sbjct: 721  SMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSALLRRHPEPMLRKLREFETFCSKHG 780

Query: 781  FELDTSSSVHFQQSLEQIRIELQDDPLLFDILISYATRPMQLATYFCSGELKDGETRSHY 840
            FELDTSSSVHFQQSLEQIRIELQDDPLLFDILISYATRPMQLATYFCSGELKDGETRSHY
Sbjct: 781  FELDTSSSVHFQQSLEQIRIELQDDPLLFDILISYATRPMQLATYFCSGELKDGETRSHY 840

Query: 841  ALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKMYLKHKGVIQKVNSNEETRCFTGIYF 900
            ALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKMYLKHKGVIQKVNSNEETRCFTGIYF
Sbjct: 841  ALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKMYLKHKGVIQKVNSNEETRCFTGIYF 900

Query: 901  DKDAADSLEGREALSSAALKHGVPCSKLLLDVALHCNDRKLASKHVADGIEKLYMWALLK 960
            DKDAADSLEGREALSSAALKHGVPCSKLLLDVALHCNDRKLASKHVADGIEKLYMWALLK
Sbjct: 901  DKDAADSLEGREALSSAALKHGVPCSKLLLDVALHCNDRKLASKHVADGIEKLYMWALLK 960

Query: 961  KKKILFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLETTSTLVLRFFCSRR 1020
            KKKILFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLETTSTLVLRFFCSRR
Sbjct: 961  KKKILFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLETTSTLVLRFFCSRR 1020

Query: 1021 SHRSRGSVKWKALEDVALVISPCDQNVKERTLGVSSNGGASKGGSAVVEQDSNLKSHVSD 1080
            SHRSRGSVKWKALEDVALVISPCDQNVKERTLGVSSNGGASKGGSAVVEQDSNLKSHVSD
Sbjct: 1021 SHRSRGSVKWKALEDVALVISPCDQNVKERTLGVSSNGGASKGGSAVVEQDSNLKSHVSD 1080

Query: 1081 TGVDPAIFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSYLR 1126
            TGVDPAIFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSYLR
Sbjct: 1081 TGVDPAIFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSYLR 1125

BLAST of Cucsa.365270 vs. NCBI nr
Match: gi|778710143|ref|XP_011656525.1| (PREDICTED: DIS3-like exonuclease 2 isoform X2 [Cucumis sativus])

HSP 1 Score: 2110.1 bits (5466), Expect = 0.0e+00
Identity = 1048/1052 (99.62%), Postives = 1049/1052 (99.71%), Query Frame = 1

Query: 74   LTQQENHSNQQIEHGLTRGDKIGFSSLPPLHINEQAELSASHNLMNQNHHSSDAGGRVTK 133
            L  +ENHSNQQIEHGLTRGDKIGFSSLPPLHINEQAELSASHNLMNQNHHSSDAGGRVTK
Sbjct: 22   LPLRENHSNQQIEHGLTRGDKIGFSSLPPLHINEQAELSASHNLMNQNHHSSDAGGRVTK 81

Query: 134  SCPEQIASGRYSGISLNQHSPPADVTDNNTQRKYFPSHWSVDDVNEGLQKGGIFKALFRV 193
            SCPEQIASGRYSGISLNQHSPPADVTDNNTQRKYFPSHWSVDDVNEGLQKGGIFKALFRV
Sbjct: 82   SCPEQIASGRYSGISLNQHSPPADVTDNNTQRKYFPSHWSVDDVNEGLQKGGIFKALFRV 141

Query: 194  NAHNRLEAYCKIDGLPIDVLINGIASQNRAVEGDIVAIKLDPFTSWTKMKGTSEAHNNMH 253
            NAHNRLEAYCKIDGLPIDVLINGIASQNRAVEGDIVAIKLDPFTSWTKMKGTSEAHNNMH
Sbjct: 142  NAHNRLEAYCKIDGLPIDVLINGIASQNRAVEGDIVAIKLDPFTSWTKMKGTSEAHNNMH 201

Query: 254  SMEDANLPAELTEKNDHNCKGKNKVDADVKSDSFRSTSLPDKRCCSEDKVLDGVACDVLL 313
            SMEDANLPAELTEKNDHNCKGKNKVDADVKSDSFRSTSLPDKRCCSEDKVLDGVACDVLL
Sbjct: 202  SMEDANLPAELTEKNDHNCKGKNKVDADVKSDSFRSTSLPDKRCCSEDKVLDGVACDVLL 261

Query: 314  SNYEQCDINELSVVNPSQAHHSSNQDDVSKAIGRICALINLYPAKRPTGRVVTILEKSRL 373
            SNYEQCDINELSVVNPSQAHHSSNQDDVSKAIGRICALINLYPAKRPTGRVVTILEKSRL
Sbjct: 262  SNYEQCDINELSVVNPSQAHHSSNQDDVSKAIGRICALINLYPAKRPTGRVVTILEKSRL 321

Query: 374  RENVVGHLNVKKFLSFQEFYVKESTKSCLSPSQNCGYVQLMPNDARFPIMMVLAGDLPNC 433
            RENVVGHLNVKKFLSFQEFYVKESTKSCLSPSQNCGYVQLMPNDARFPIMMVLAGDLPNC
Sbjct: 322  RENVVGHLNVKKFLSFQEFYVKESTKSCLSPSQNCGYVQLMPNDARFPIMMVLAGDLPNC 381

Query: 434  IKKRLDNGDVTVENELVAARIYEWVKESSSPRAHVLHVLGRGNEVESHIDAILFENAFRT 493
            IKKRLDNGDVTVENELVAARIYEWVKESSSPRAHVLHVLGRGNEVESHIDAILFENA RT
Sbjct: 382  IKKRLDNGDVTVENELVAARIYEWVKESSSPRAHVLHVLGRGNEVESHIDAILFENAIRT 441

Query: 494  CEFSQDSLSCVPQTPWKIPPEELQCRRDIRNLCIFTIDPSSASDLDDALSVQRLANGIFR 553
            CEFSQDSLSCVPQTPWKIPPEELQCRRDIRNLCIFTIDPSSASDLDDALSVQRLANGIFR
Sbjct: 442  CEFSQDSLSCVPQTPWKIPPEELQCRRDIRNLCIFTIDPSSASDLDDALSVQRLANGIFR 501

Query: 554  VGIHIADVSYFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLSESIGSLNPGVDRLAFS 613
            VGIHIADVSYFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLSESIGSLNPGVDRLAFS
Sbjct: 502  VGIHIADVSYFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLSESIGSLNPGVDRLAFS 561

Query: 614  LFLDINSCGDVKDFWIERTVICCCCKLSYEHAQDIIDGLIDSDSSELFGNNCPQLHGQFT 673
            LFLDINSCGDVKDFWIERTVICCCCKLSYEHAQDIIDGLIDSDSSELFGNNCPQLHGQFT
Sbjct: 562  LFLDINSCGDVKDFWIERTVICCCCKLSYEHAQDIIDGLIDSDSSELFGNNCPQLHGQFT 621

Query: 674  WHDVISSVKLLHEISKTVKEKRFRNGALRLENSKLIYLYDEYGIPYDSMFYEQKDSNFLV 733
            WHDVISSVKLLHEISKTVKEKRFRNGALRLENSKLIYLYDEYGIPYDSMFYEQKDSNFLV
Sbjct: 622  WHDVISSVKLLHEISKTVKEKRFRNGALRLENSKLIYLYDEYGIPYDSMFYEQKDSNFLV 681

Query: 734  EEFMLLANRTVAEVISRTFPDSALLRRHPEPMLRKLREFETFCSKHGFELDTSSSVHFQQ 793
            EEFMLLANRTVAEVISRTFPDSALLRRHPEPMLRKLREFETFCSKHGFELDTSSSVHFQQ
Sbjct: 682  EEFMLLANRTVAEVISRTFPDSALLRRHPEPMLRKLREFETFCSKHGFELDTSSSVHFQQ 741

Query: 794  SLEQIRIELQDDPLLFDILISYATRPMQLATYFCSGELKDGETRSHYALAVPLYTHFTSP 853
            SLEQIRIELQDDPLLFDILISYATRPMQLATYFCSGELKDGETRSHYALAVPLYTHFTSP
Sbjct: 742  SLEQIRIELQDDPLLFDILISYATRPMQLATYFCSGELKDGETRSHYALAVPLYTHFTSP 801

Query: 854  LRRYPDIVVHRTLAAAIEAEKMYLKHKGVIQKVNSNEETRCFTGIYFDKDAADSLEGREA 913
            LRRYPDIVVHRTLAAAIEAEKMYLKHKGVIQKVNSNEETRCFTGIYFDKDAADSLEGREA
Sbjct: 802  LRRYPDIVVHRTLAAAIEAEKMYLKHKGVIQKVNSNEETRCFTGIYFDKDAADSLEGREA 861

Query: 914  LSSAALKHGVPCSKLLLDVALHCNDRKLASKHVADGIEKLYMWALLKKKKILFSDARVLG 973
            LSSAALKHGVPCSKLLLDVALHCNDRKLASKHVADGIEKLYMWALLKKKKILFSDARVLG
Sbjct: 862  LSSAALKHGVPCSKLLLDVALHCNDRKLASKHVADGIEKLYMWALLKKKKILFSDARVLG 921

Query: 974  LGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLETTSTLVLRFFCSRRSHRSRGSVKWKAL 1033
            LGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLETTSTLVLRFFCSRRSHRSRGSVKWKAL
Sbjct: 922  LGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLETTSTLVLRFFCSRRSHRSRGSVKWKAL 981

Query: 1034 EDVALVISPCDQNVKERTLGVSSNGGASKGGSAVVEQDSNLKSHVSDTGVDPAIFPLTVR 1093
            EDVALVISPCDQNVKERTLGVSSNGGASKGGSAVVEQDSNLKSHVSDTGVDPAIFPLTVR
Sbjct: 982  EDVALVISPCDQNVKERTLGVSSNGGASKGGSAVVEQDSNLKSHVSDTGVDPAIFPLTVR 1041

Query: 1094 LLSTIPVALHAVGGDDGPIDIGVRLYMSSYLR 1126
            LLSTIPVALHAVGGDDGPIDIGVRLYMSSYLR
Sbjct: 1042 LLSTIPVALHAVGGDDGPIDIGVRLYMSSYLR 1073

BLAST of Cucsa.365270 vs. NCBI nr
Match: gi|659089736|ref|XP_008445669.1| (PREDICTED: LOW QUALITY PROTEIN: DIS3-like exonuclease 2 [Cucumis melo])

HSP 1 Score: 2093.9 bits (5424), Expect = 0.0e+00
Identity = 1037/1125 (92.18%), Postives = 1081/1125 (96.09%), Query Frame = 1

Query: 1    MRAAVEQSTPERNGDGDKEKKKKRRSNRRSKHNPSLTTSASYTSVNGILGEASECMENGR 60
            MRAA EQSTPERNGD DKEKKKKRRSNRRSKHNPSLTTSASYTSVNGILGEASECMENGR
Sbjct: 1    MRAAFEQSTPERNGDCDKEKKKKRRSNRRSKHNPSLTTSASYTSVNGILGEASECMENGR 60

Query: 61   IDANLTSPSNYSSLTQQENHSNQQIEHGLTRGDKIGFSSLPPLHINEQAELSASHNLMNQ 120
            IDANLTSPSNYSSLTQQENHSN  IEHGLT G+KI FSSLP LHIN+QAELSAS NL+NQ
Sbjct: 61   IDANLTSPSNYSSLTQQENHSNHPIEHGLTGGNKIAFSSLPSLHINDQAELSASQNLINQ 120

Query: 121  NHHSSDAGGRVTKSCPEQIASGRYSGISLNQHSPPADVTDNNTQRKYFPSHWSVDDVNEG 180
            NHHSSDAGGR+ KSCPEQIASGR SGIS NQ SPPAD+T+NNTQRKYFPSHWS+DDVNEG
Sbjct: 121  NHHSSDAGGRIIKSCPEQIASGRNSGISSNQLSPPADLTENNTQRKYFPSHWSIDDVNEG 180

Query: 181  LQKGGIFKALFRVNAHNRLEAYCKIDGLPIDVLINGIASQNRAVEGDIVAIKLDPFTSWT 240
            LQKG IFKALFRVNAHNRLEAYCKIDGLP+DVLINGIASQNRAVEGDIVAIK+DPFT WT
Sbjct: 181  LQKGDIFKALFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIKVDPFTVWT 240

Query: 241  KMKGTSEAHNNMHSMEDANLPAELTEKNDHNCKGKNKVDADVKSDSFRSTSLPDKRCCSE 300
            KMKGTSEAH+N+ SM+DANLPAE TEKN HNCKGKNK DAD KSDSFRS+SLPDKRCCSE
Sbjct: 241  KMKGTSEAHDNIKSMDDANLPAEPTEKNSHNCKGKNKFDADGKSDSFRSSSLPDKRCCSE 300

Query: 301  DKVLDGVACDVLLSNYEQCDINELSVVNPSQAHHSSNQDDVSKAIGRICALINLYPAKRP 360
            DKVLDG++CD LLSNYEQCDIN+LSVV+PSQAHHSSNQ DVSK IGRICALINLYPAKRP
Sbjct: 301  DKVLDGISCDDLLSNYEQCDINQLSVVDPSQAHHSSNQYDVSKIIGRICALINLYPAKRP 360

Query: 361  TGRVVTILEKSRLRENVVGHLNVKKFLSFQEFYVKESTKSCLSPSQNCGYVQLMPNDARF 420
            TGRVVTILEKSRLR+NVVGHLNVKKFLSFQEFYVKE+TKSCLSPSQN GYVQLMPNDARF
Sbjct: 361  TGRVVTILEKSRLRDNVVGHLNVKKFLSFQEFYVKENTKSCLSPSQNGGYVQLMPNDARF 420

Query: 421  PIMMVLAGDLPNCIKKRLDNGDVTVENELVAARIYEWVKESSSPRAHVLHVLGRGNEVES 480
            PIMMVLAGDLP+CIKKRLDNGDVTVENELVAARIY+WVKESSSPRAHVLHVLGRG+EVES
Sbjct: 421  PIMMVLAGDLPDCIKKRLDNGDVTVENELVAARIYDWVKESSSPRAHVLHVLGRGSEVES 480

Query: 481  HIDAILFENAFRTCEFSQDSLSCVPQTPWKIPPEELQCRRDIRNLCIFTIDPSSASDLDD 540
            HIDAILFENA RTCEFS DSLSC+P TPWKIP EEL+CRRDIRNLCIFTIDPSSASDLDD
Sbjct: 481  HIDAILFENAIRTCEFSHDSLSCIPHTPWKIPHEELRCRRDIRNLCIFTIDPSSASDLDD 540

Query: 541  ALSVQRLANGIFRVGIHIADVSYFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLSESI 600
            ALSVQ+LAN IFRVGIHIADVSYFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLSE+I
Sbjct: 541  ALSVQKLANDIFRVGIHIADVSYFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLSENI 600

Query: 601  GSLNPGVDRLAFSLFLDINSCGDVKDFWIERTVICCCCKLSYEHAQDIIDGLIDSDSSEL 660
            GSLNPGVDRLAFSLFLDIN CGDVKD+WIERTVICCCCKLSYE+AQDIIDGLIDSDS E+
Sbjct: 601  GSLNPGVDRLAFSLFLDINGCGDVKDYWIERTVICCCCKLSYEYAQDIIDGLIDSDSPEI 660

Query: 661  FGNNCPQLHGQFTWHDVISSVKLLHEISKTVKEKRFRNGALRLENSKLIYLYDEYGIPYD 720
            FGNNCPQLHGQFTWHDVISSVKLLHEISKT+KEKRFR+GALRLENSKLIYLYDEYGIPYD
Sbjct: 661  FGNNCPQLHGQFTWHDVISSVKLLHEISKTLKEKRFRDGALRLENSKLIYLYDEYGIPYD 720

Query: 721  SMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSALLRRHPEPMLRKLREFETFCSKHG 780
            SMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSALLRRHPEPMLRKLREFE+FCSKHG
Sbjct: 721  SMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSALLRRHPEPMLRKLREFESFCSKHG 780

Query: 781  FELDTSSSVHFQQSLEQIRIELQDDPLLFDILISYATRPMQLATYFCSGELKDGETRSHY 840
            FELDTSSSVHFQQSLEQIR +L DDPLLFDILISYATRPMQLATYFCSGELKDGE R+HY
Sbjct: 781  FELDTSSSVHFQQSLEQIRTKLHDDPLLFDILISYATRPMQLATYFCSGELKDGEKRNHY 840

Query: 841  ALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKMYLKHKGVIQKVNSNEETRCFTGIYF 900
            ALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEK+YLKH+G+IQKVNS++E RCFTGIYF
Sbjct: 841  ALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKVYLKHQGIIQKVNSDKEMRCFTGIYF 900

Query: 901  DKDAADSLEGREALSSAALKHGVPCSKLLLDVALHCNDRKLASKHVADGIEKLYMWALLK 960
            DKDAADSLEGREALS AALKHGVPCSKLL DVALHCNDRKLASKH+ADG EKLYMWALLK
Sbjct: 901  DKDAADSLEGREALSFAALKHGVPCSKLLSDVALHCNDRKLASKHIADGCEKLYMWALLK 960

Query: 961  KKKILFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLETTSTLVLRFFCSRR 1020
            KK+ILFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLAVEWL+TTSTLVL  FCSRR
Sbjct: 961  KKRILFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLDTTSTLVLS-FCSRR 1020

Query: 1021 SHRSRGSVKWKALEDVALVISPCDQNVKERTLGVSSNGGASKGGSAVVEQDSNLKSHVSD 1080
            SHRSRGSVKWKALEDVALVISPCDQNV +RTLGV  NGGASKGGSA VEQDSNLKSHVSD
Sbjct: 1021 SHRSRGSVKWKALEDVALVISPCDQNVNKRTLGVCPNGGASKGGSAAVEQDSNLKSHVSD 1080

Query: 1081 TGVDPAIFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSYLR 1126
             GVDPA+FPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSYLR
Sbjct: 1081 IGVDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSYLR 1124

BLAST of Cucsa.365270 vs. NCBI nr
Match: gi|645225814|ref|XP_008219749.1| (PREDICTED: DIS3-like exonuclease 2 [Prunus mume])

HSP 1 Score: 1244.6 bits (3219), Expect = 0.0e+00
Identity = 652/1129 (57.75%), Postives = 822/1129 (72.81%), Query Frame = 1

Query: 1    MRAAVEQSTPERNGDGDKEKKKKRRSNRRSKHNPSLTTSASYTSVNGILGEASECMENGR 60
            M+ AV Q+  +R  DGDKEKKKK+R  RRSK N S  TSAS  S N I  + SEC+ NGR
Sbjct: 1    MKGAVVQAVVDRVEDGDKEKKKKQR-RRRSKQNSS--TSAS-NSANEIRSDVSECLGNGR 60

Query: 61   IDANLTSPSNYSSLTQQENHSNQQIEHGLTRGDKIGFSSLPPLHINEQAELSASHNLMNQ 120
               ++T P     L Q +   +   EHG+ +     FSSLP +HINEQ       +L NQ
Sbjct: 61   TSDHVTIP-----LKQHQIVLHPPYEHGMNKASNFPFSSLPTMHINEQENPEDVQSLENQ 120

Query: 121  NHHSSDAGGRV-TKSCPEQIASGRYSGISLNQHSPPADVTDNNTQRKYFPSHWSVDDVNE 180
            +    D G RV   SCPE +A G   GI + +  PP  + +   +RKYF  +WS++  N+
Sbjct: 121  HSLPCDPGRRVFANSCPESVACGESPGIFIRKDFPPHHI-EGYARRKYFTPYWSMEATND 180

Query: 181  GLQKGGIFKALFRVNAHNRLEAYCKIDGLPIDVLINGIASQNRAVEGDIVAIKLDPFTSW 240
             ++KG  FKALFRVNAHNR EAYCK+DG+P DVLI G+A QN+AVEGDIV +K+DP   W
Sbjct: 181  AIEKGEAFKALFRVNAHNRHEAYCKVDGVPTDVLIGGLAEQNKAVEGDIVVVKVDPLPLW 240

Query: 241  TKMKGTSEAHNNMHSMEDANLPAELTEKNDHNCKGKNKVDADVKSDSFRSTSLPDKRCCS 300
            T+MKG++    +   ++D N   E      +NCKGK KVD      + RS+ LP++    
Sbjct: 241  TRMKGSAGTCTSSAPVDDFNFQLENNVIAGYNCKGKAKVDEVYLYGNDRSSLLPERGSRP 300

Query: 301  EDKVLDGVACDVLLSNYEQCDINELSVVNPS-QAHHSSNQDDVSKAIGRICALINLYPAK 360
            E+ V +      +  +          + + S QA  S  Q++V  ++ R+CA+IN +P+K
Sbjct: 301  EESVGESFHSGPIGQSIYDHVAGRYPLPSDSIQAGSSPEQNEVRLSVERLCAMINSFPSK 360

Query: 361  RPTGRVVTILEKSRLRENVVGHLNVKKFLSFQEFYVKESTKSCLSPSQNCGYVQLMPNDA 420
            RPTGRVV I+E+S  R+ +VG LNVK+++S++EF  K+  K+  S   N  ++Q+ P D 
Sbjct: 361  RPTGRVVAIVERSPRRDAIVGFLNVKQWISYREFCRKDMRKNKNSSFSNHEHIQMTPIDP 420

Query: 421  RFPIMMVLAGDLPNCIKKRLDNGDVTVENELVAARIYEWVKESSSPRAHVLHVLGRGNEV 480
            RFP M+VL  +LP+ IKKRL+NGD T+E EL AARI EW +ESS+P+A +L+  GRG E+
Sbjct: 421  RFPKMVVLVRNLPDSIKKRLENGDETIEMELFAARIDEWDEESSAPQAVILNAFGRGCEL 480

Query: 481  ESHIDAILFENAFRTCEFSQDSLSCVPQTPWKIPPEELQCRRDIRNLCIFTIDPSSASDL 540
            +  I+AILF+NA  + EFS +SLSC+P  PW++P EE Q RRD+RN CIFTIDPS+A+DL
Sbjct: 481  QPQIEAILFQNAINSSEFSPESLSCLPHLPWEVPQEEFQTRRDLRNWCIFTIDPSTATDL 540

Query: 541  DDALSVQRLANGIFRVGIHIADVSYFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLSE 600
            DDALSV +L+NGI+RVGIHIADVS+FVLP T LD+EAQ RSTSVY+ +RK+PMLPPLLSE
Sbjct: 541  DDALSVDKLSNGIYRVGIHIADVSHFVLPGTPLDEEAQSRSTSVYMSRRKLPMLPPLLSE 600

Query: 601  SIGSLNPGVDRLAFSLFLDINSCGDVKDFWIERTVICCCCKLSYEHAQDIIDGLIDSDSS 660
            ++GSLNPGV+RLAFS+F+D+N  GDV D WI RTVI  CCKLSYEHAQDIIDG  + +S 
Sbjct: 601  NVGSLNPGVERLAFSIFVDMNHAGDVVDRWIGRTVIRSCCKLSYEHAQDIIDGKFNLESV 660

Query: 661  ELFGNNCPQLHGQFTWHDVISSVKLLHEISKTVKEKRFRNGALRLENSKLIYLYDEYGIP 720
            ++ GN  PQLHG F W DV+ SVK L EIS+ +KE+RF +GAL+LE+SK++ L+DEYG+P
Sbjct: 661  DILGNGRPQLHGHFEWFDVLRSVKDLLEISRILKERRFSDGALQLESSKVVILFDEYGVP 720

Query: 721  YDSMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSALLRRHPEPMLRKLREFETFCSK 780
            YDSM  E+K+SNFLVEEFMLLANRT AEVISR FPDSALLRRHPEP LRKLREFE FCSK
Sbjct: 721  YDSMHSERKESNFLVEEFMLLANRTAAEVISRAFPDSALLRRHPEPNLRKLREFEAFCSK 780

Query: 781  HGFELDTSSSVHFQQSLEQIRIELQDDPLLFDILISYATRPMQLATYFCSGELKDGETR- 840
            HG ELDTSSS  FQ SLE+IR +L+DD +LF+IL++YAT+PMQLA YFCSGELKD E   
Sbjct: 781  HGLELDTSSSGQFQLSLEKIREKLKDDCILFNILMNYATKPMQLAAYFCSGELKDKENDW 840

Query: 841  SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKMYLKHKGVIQKVNSNEETR--CF 900
             HY LAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAE++ LKH+ ++   N  +E R  CF
Sbjct: 841  GHYGLAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEELLLKHRRMLNNFNRGDECRMKCF 900

Query: 901  TGIYFDKDAADSLEGREALSSAALKHGVPCSKLLLDVALHCNDRKLASKHVADGIEKLYM 960
            TGIYFDKDAA+S E RE LS+A++KH +PCS+LL DVA +CN+RKLAS+HV D  +KLYM
Sbjct: 901  TGIYFDKDAAESYESREVLSAASMKHRIPCSELLTDVAAYCNERKLASRHVKDACDKLYM 960

Query: 961  WALLKKKKILFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLETTSTLVLRF 1020
            WALLKKK+IL S+AR++GLGPRFMS+YI KLA+ERRIYYDEVEGL VEWL+ TST+VL  
Sbjct: 961  WALLKKKEILLSEARIMGLGPRFMSIYIHKLAVERRIYYDEVEGLMVEWLDATSTVVLTL 1020

Query: 1021 FCSRRSHRSRGSVKWKALEDVALVISPCDQNVKERTLGVSSNGGASKGGSAVVEQDSNLK 1080
              +RRS R     K +ALEDVALVI P D   +   +G S+N GA+     V    SN  
Sbjct: 1021 CSNRRSLRRGSPGKCRALEDVALVIRPYDLKAELGAVGNSTNEGAAAQDVGVATHSSN-- 1080

Query: 1081 SHVSDTGVDPAIFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSYL 1125
                ++ +DP +FPLT+R+LSTI V LHA+GGDDGPIDIG RLYMSSYL
Sbjct: 1081 ----ESEIDPLVFPLTLRVLSTISVVLHAIGGDDGPIDIGARLYMSSYL 1113

BLAST of Cucsa.365270 vs. NCBI nr
Match: gi|657986858|ref|XP_008385571.1| (PREDICTED: DIS3-like exonuclease 2 isoform X1 [Malus domestica])

HSP 1 Score: 1238.8 bits (3204), Expect = 0.0e+00
Identity = 642/1131 (56.76%), Postives = 829/1131 (73.30%), Query Frame = 1

Query: 1    MRAAVEQSTPERNGDGDKEKKKKRRSNRRSKHNPSLTTSASYTSVNGILGEASECMENGR 60
            M+  V Q+  +R  DGDKEKKKKRR NRRS+ N S + ++  T       E SEC+ NGR
Sbjct: 1    MKDXVVQAVVKRVEDGDKEKKKKRRPNRRSRQNNSTSAASPRT-------EVSECLANGR 60

Query: 61   IDANLTSPSNYSSLTQQENHSNQQIEHGLTRGDKIGFSSLPPLHINEQAELSASHNLMNQ 120
            I  ++T+     SL Q +   +   EHG+ +   + F+SLP +HINEQ        L+NQ
Sbjct: 61   ISNHVTT-----SLKQPQLDMHPPYEHGMIKASNLAFNSLPTMHINEQVNHEDVQILVNQ 120

Query: 121  NHHSSDAGGR-VTKSCPEQIASGRYSGISLNQHSPPADVTDNNTQRKYFPSHWSVDDVNE 180
            +    D  GR ++ SCPE +A G   G+  +    P    ++  +RK F SHW ++ VN+
Sbjct: 121  HSLPCDPAGRFISNSCPEPVACGGSPGMFKDF---PPHHXESCARRKCFTSHWPMEAVND 180

Query: 181  GLQKGGIFKALFRVNAHNRLEAYCKIDGLPIDVLINGIASQNRAVEGDIVAIKLDPFTSW 240
             L+KG  FKALFRVNAHNRLEAYCKIDG+P D+LI+G+A QNRA+EGDIVAIK+DP   W
Sbjct: 181  ALEKGDAFKALFRVNAHNRLEAYCKIDGVPTDILIDGLAEQNRAMEGDIVAIKVDPLALW 240

Query: 241  TKMKGTSEAHNNMHSMEDANLPAELTEKNDHNCKGKNKVDADVKSDSFRSTSLPDKRCCS 300
             +MKG++    +   +ED NLP +  E +  NCKGK KVD      + R + LP++  C 
Sbjct: 241  MRMKGSAGTCTSSALVEDFNLPQQANEISGLNCKGKAKVDEVDLYANGRRSLLPERXSCP 300

Query: 301  EDKVLDG---VACDVLLSNYEQCDINELSVVNPSQAHHSSNQDDVSKAIGRICALINLYP 360
            ++    G    +  +  ++Y+       S  +  Q      Q++V+ AI RICA+I+ +P
Sbjct: 301  QESTYPGESITSXPIGQTSYDHVAGRYPSASDFLQEGSHGEQNEVAVAIERICAMISSFP 360

Query: 361  AKRPTGRVVTILEKSRLRENVVGHLNVKKFLSFQEFYVKESTKSCLSPSQNCGYVQLMPN 420
            +KRPTGRVV I+E+S  R++VVG L+VKK+++++E    +  K+  +   +  Y+Q++P 
Sbjct: 361  SKRPTGRVVAIIERSPRRDSVVGFLHVKKWIAYREVCRNDMGKNKNASFSSDEYIQMIPT 420

Query: 421  DARFPIMMVLAGDLPNCIKKRLDNGDVTVENELVAARIYEWVKESSSPRAHVLHVLGRGN 480
            D RFP M+VL   LP+ IKKRL+NGD ++E EL AARI EW +ESS+P+A +L+  GR +
Sbjct: 421  DPRFPKMVVLVRTLPDSIKKRLENGDESIEMELFAARIDEWDEESSAPQAVILNAFGRAS 480

Query: 481  EVESHIDAILFENAFRTCEFSQDSLSCVPQTPWKIPPEELQCRRDIRNLCIFTIDPSSAS 540
            EV+  I+AILF+N+  + +FS +SLSC+P   W++P EE++ R+D+RNLCI TIDPS+A+
Sbjct: 481  EVQPQIEAILFQNSINSSDFSHESLSCLPHLTWEVPQEEIKSRKDLRNLCILTIDPSTAT 540

Query: 541  DLDDALSVQRLANGIFRVGIHIADVSYFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLL 600
            DLDDALSV++L+NGIFRVGIHIADVSYFVLPDT LD+EAQ RSTSVY+ QRK+PMLPPLL
Sbjct: 541  DLDDALSVEKLSNGIFRVGIHIADVSYFVLPDTPLDEEAQSRSTSVYMSQRKLPMLPPLL 600

Query: 601  SESIGSLNPGVDRLAFSLFLDINSCGDVKDFWIERTVICCCCKLSYEHAQDIIDGLIDSD 660
            SE+IGSLNPGV+RLAFS+FLDIN  GDV D WI RTVI  CCKLSYEHAQ+II+G ++ +
Sbjct: 601  SENIGSLNPGVERLAFSIFLDINYVGDVVDRWIGRTVIRSCCKLSYEHAQEIINGKLNLE 660

Query: 661  SSELFGNNCPQLHGQFTWHDVISSVKLLHEISKTVKEKRFRNGALRLENSKLIYLYDEYG 720
            SS + G   PQLHG F W DVI SVK L EIS+ +KE+RF +GAL+LE+SK++ L+DEYG
Sbjct: 661  SSNILGKGFPQLHGHFEWSDVIRSVKDLLEISRVLKERRFSDGALQLESSKIVILFDEYG 720

Query: 721  IPYDSMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSALLRRHPEPMLRKLREFETFC 780
            +PYDSM+ E+KDSNFLVEEFMLLAN T AEVISR FP+SALLRRHPEP +RKLREFE FC
Sbjct: 721  VPYDSMYSERKDSNFLVEEFMLLANTTAAEVISRAFPESALLRRHPEPNMRKLREFEAFC 780

Query: 781  SKHGFELDTSSSVHFQQSLEQIRIELQDDPLLFDILISYATRPMQLATYFCSGELKDGET 840
            SKHG E DTSSS  FQQSL +IR EL+DD +LF+IL+SYAT+PMQLA+YFCSGELKD E 
Sbjct: 781  SKHGLEXDTSSSGXFQQSLLRIREELKDDSVLFNILMSYATKPMQLASYFCSGELKDREN 840

Query: 841  R-SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKMYLKHKGVIQKVNSNEET--R 900
               HY LAVPLYTHFTSPLRRYPDIVVHRTLAAA+EAE+++LKH+ ++   +  +E   R
Sbjct: 841  DWGHYGLAVPLYTHFTSPLRRYPDIVVHRTLAAAVEAEELFLKHRRMLNNFSRGDEVKMR 900

Query: 901  CFTGIYFDKDAADSLEGREALSSAALKHGVPCSKLLLDVALHCNDRKLASKHVADGIEKL 960
            CFTGIYFDK+ A+S E +EALS+AA+KH VPCS++L DVA +CN RKLAS+HV D  +KL
Sbjct: 901  CFTGIYFDKNVAESCEIKEALSAAAIKHRVPCSEMLADVAAYCNARKLASRHVKDACDKL 960

Query: 961  YMWALLKKKKILFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLETTSTLVL 1020
            YMWALLKKK+I+ S+ARVLG+GPRFMS+YI KLA+ERRIYYDEVEGL VEWL+ TSTLVL
Sbjct: 961  YMWALLKKKEIVLSEARVLGVGPRFMSIYIHKLAVERRIYYDEVEGLMVEWLDATSTLVL 1020

Query: 1021 RFFCSRRSHRSRGSVKWKALEDVALVISPCDQNVKERTLGVSSNGGASKGGSAVVEQDSN 1080
                +RRSHR     KW+ALEDVALV+ P D   +     +   G +SK G+ V  QD  
Sbjct: 1021 NLCSNRRSHRRGSPGKWRALEDVALVVRPYDLKAE-----LGGTGNSSKEGAVV--QDVG 1080

Query: 1081 LKSHVSDTGVDPAIFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSYL 1125
            + +H   + +D  +FPLT+R+LST PV LHA+GGD GP+DIG RLY+SSYL
Sbjct: 1081 VATH---SEIDAVVFPLTLRVLSTFPVVLHAIGGDYGPVDIGARLYVSSYL 1106