Cucsa.363400 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.363400
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Prolyl 4-hydroxylase alpha subunit, putative
Location	scaffold03611 : 966853 .. 970593 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTCTCTCTCTCTCCCTCTCGCGCGCGCGCTCTTTCTAATTTGATCCGATCGAGACTATGTTCAAATTTGATAATCTGTTATTCATCTTCTTGATTTTGACCTCATCGTTTATTCGGGAATCAACTTGTTCTTATGCTGGTTCGGCTAGCGCAACCGTAGATCCTAGTAAAGTGAAGCAGATTTCATGGAAACCGAGGTGTTTATTGCTAGCATTTTATAACGAACGGATCTTCTTTTTGTTTTCTTCGTTCTATTGTGTTTGTTTTCTGAGAAAGTTGTGGAACGGAAATTATTCAACTCTAACTTGTTTTAGCTTCTAGCTTCCGGTCGTTTAGGTTTAGTTCAGTGGCTATTGATAGTAATTTATGCTTTTCAAATTCTTGATAAGGCGATTTCACTGAATGTTGCAGAGCTTTTGTATATGAAGGTTTTCTAACGGACCTAGAATGCGACCATCTGGTTTCTATAGTAAGTTTAGATTTAGTGATATTCATTGTTCAGTTTGATATGGAAGTCATTTCGTTTCGTCAATACAAATCTATTGTTGAAGGATTGTTTTTCTTCTGTGACAGGCGAGATCCGAGCTAAAGAGATCTGAGGTTGCTGATAATGATTCGGGAAAGAGTAAGCTCAGTACTGTACGAACGAGTTCAGGAATGTTCATTTCTAAAAACAAGGTTAGGTTGAATCTCCACACTAACAATCTTCTAAGTTTTCTGGATGTTTTCTCTAATTTCTCTTTCGATTTTTGTTTTTGATCGGAATCGAGGTTCTGTTACTAGTCGTCACCGAAGCTATTTATTGAATTAAACTTAATGGAAGAATCTTATCCGTTGCGAAACTGAAATATATTGCCATTTCAAAGAGATTAAACGGCAGGAATGTTCCTGATAGTATGGATGCAATAATCAACATTACGTTCATTCAAAATTAGAGAGTAAACAATCTTTCAGGAATCATAACTTAAAACCTCCTCCTCTTCTATCTTGTTGAATTAGGACTAGATTAACAAGTGTGTATTTTGTGCTCTTCACCTGTATCTTAAAATTGATGTATATAGCAGGACAATAATTATTTCCTTTGGTTCTGTTGAGTATCAGTTGAGATCTTTACACCCATGTTTGTATCGAATATTTACATTTTGATTTGATATTTAGGATCCTATTGTTTCTGGCATAGAGGACAAAATTTCTGCATGGACTTTTCTTCCAAAAGGTACACGTTCACTGTCAGATCACATATTTTGTAATTGATATGTTATGTCATATGCTTTTGCTTTACTAGAGCTGATTGCCATAAGTAACATTCATGGTTTCTTTATAATGAAATGGTTGTCAATGTCGAGTATCACTGCTAAATGCCGTGTGTATTTCTCGTTTTTCAGAAAATGGGGAGGACATTCAGGTATTGAGATACGAGCATGGGCAGAAATATGAGTCACATTATGATTACTTTGTTGACAAGGTGAATATTGCCTGGGGTGGACATCGTTTAGCTACGGTCCTTATGTATCTCTCTAATGTGACCCAAGGTGGTGAAACAGTTTTCCCCTTGGCAGAGGTTAGTTCAAGCGTTAATTCGTACTTTACTAGAATTGCACTTTATATTCCTCATCGTAAGACTACTTCTTCTTAGATCTCAGTACTCTCTAAATAATAATCATACAACTCTGTCTTCTGATGGAGAAGATCCTTTTATAAGACTGATACGTGGAAATATTGAAATGAAACATGAGAAAGACTCGATGTGTTGGATAATCCAGAGGGTGTTGCCATTATTTTATAGGAATCACAACTGGAAATGACCTGATTTTTGTTCAGGTTGATGACACCGAACCCTTTTACCCAATCTGAACCATTTTTCTGTTTCTTTTGTTTAATTTTCTTATTTTTTGGATTTTGTTTTTCTATCTAAATGTTTATCAATCGATCTATATTCATTATGGATGCAATACAATCCTGCTTAGAGGGATTTAGCACTTTTTGCTACCATCATTTTAAGTACTTCAGTTAGGAAAAATGTTATTGCAGTCATTTTAAAGCACCGAAATTCACCAATTATCTAAAGATTTGAACCCAACTATTGTTGAATTGGATTAGACATGAAACATCTGAGATTATTGAAGTTGTTCCTTGCTGAGAAGCCAACTTGAAACAAACTTCTATAATTAGTTATGAAGTTACATATAGATTGGAAATGGATTTGATTCAAAAATTACTTTAATCAATAGCAAACCTGTTTAACCTCGTTGTATAACCTCGGAGCAGAAACCTTCCCACCGGAGAGCTTATGAAACAGACGAGGACCTCTCAGAGTGCGCTAAGAAAGGAGTTGCAGGTGAATCACCAAACAAACTATAAATGCTTCTTTTTCGCTACTTATAAAAAACTATAAAACTTTCATGTAATTAATTGATGTACTTTGTTATTTAACATGATTCGCTTGGAATGATGTAGTGAAACCAAAGAAAGGCGATGCCCTTCTTTTCTTTAGCCTTGAACCAAATGCTATACCAGACACAAACAGTCTCCATGGAGGTTGCCCTGTCCTTGAAGGAGAAAAATGGTCAGCAACAAAGTGGATTCACGTAGATTCTTTCAGCAAAAACTTAGGAGACATTGGGAATTGTACTGATCTAAATGAAAGTTGTGAGAGATGGGCTGCCTTAGGGGAATGCACCAAAAACCCAGAATATATGGTTGGATCTCCAGAAATGCCCGGCTACTGTCGGAGGAGTTGCAGGATCTGTTGATTTCATTTCAACCTTACACTCGAAATTCCGATACCTTTTCGTGGTAAACTTCTTATTCCTTGGTTGGATAGTAGTAATCAATTTGTTGCTAAGCTTTCGTTCAAACCATTTGGTTTTTAGTTTTTGAAAATTAAGCCTTTAAATACTTATTTCTCGACTAAATTTCTTGTTTTGTAATGATTATTTATCTATATTTTCAAAAACCAAGTCAAATTTTAGAATTTAAAAACAATAGAGTTTTTTAAAAATAACAAAATATGAAACTATTTACGAAATATAACAAAATTGAGAATGATACATACTATTGATTTTTGTTTTCGGAATTTGACAAAGATTTCAATTACCATCGTAAAAAATGAACATGAGTTTTTGAAAATAAAAAAAAACAAATTGGTTACTGGATGAGACCGAATTATTGGCTTTTAAGTTTCGGAATTTCTTTTCGTTTGCATGGCACTTCAATTTGTTTAGGCTTTCCCTGTTACCTACACTCAATTGTCATATGTTTCCTTCCAAAAATGAAATAGCTTAAATTGCAAGTTTAGTACCCATAGTAGGAAAGAAGTTAGAATTCAGCTCATGGCTTATAATGTAAATTTAGTTTTTATGGTAGCACTGTTTATGAAAATTTTATGGACCTATAAAGACTAAAGTCTTAACCTTTAAAATAAGGATTAAATTCCAACTTACTCTAGAATCAATTTTGTAATTTAATAGAAAGATATAACTCTTAGATAAAGCTTTGTCCCTCATGAATTTCTTTACCTTTTTTTTTTCTTTTTTCTTTTTTCTTTTTTGGTTTTGGTGTCATTTGGAAAGTCAGTCATTTGTGGAATGGAAAGCGATACAACATTGATTGTGAAGCTATCGTGGAAGCACAGATGGGATGAAGCAGTAGCCGTTGGGTAATTATGACCTTTCCTTTTTCTTTAAAAAAAATTCATGATTATGTATTACTTTATATTGTCGTTATTTTTCTTACTTGAAT

mRNA sequence

tctctctctctctccctctcGCGCGCGCGCTCTTTCTAATTTGATCCGATCGAGACTATGTTCAAATTTGATAATCTGTTATTCATCTTCTTGATTTTGACCTCATCGTTTATTCGGGAATCAACTTGTTCTTATGCTGGTTCGGCTAGCGCAACCGTAGATCCTAGTAAAGTGAAGCAGATTTCATGGAAACCGAGAGCTTTTGTATATGAAGGTTTTCTAACGGACCTAGAATGCGACCATCTGGTTTCTATAGCGAGATCCGAGCTAAAGAGATCTGAGGTTGCTGATAATGATTCGGGAAAGAGTAAGCTCAGTACTGTACGAACGAGTTCAGGAATGTTCATTTCTAAAAACAAGGATCCTATTGTTTCTGGCATAGAGGACAAAATTTCTGCATGGACTTTTCTTCCAAAAGAAAATGGGGAGGACATTCAGGTATTGAGATACGAGCATGGGCAGAAATATGAGTCACATTATGATTACTTTGTTGACAAGGTGAATATTGCCTGGGGTGGACATCGTTTAGCTACGGTCCTTATGTATCTCTCTAATGTGACCCAAGGTGGTGAAACAGTTTTCCCCTTGGCAGAGAAACCTTCCCACCGGAGAGCTTATGAAACAGACGAGGACCTCTCAGAGTGCGCTAAGAAAGGAGTTGCAGTGAAACCAAAGAAAGGCGATGCCCTTCTTTTCTTTAGCCTTGAACCAAATGCTATACCAGACACAAACAGTCTCCATGGAGGTTGCCCTGTCCTTGAAGGAGAAAAATGGTCAGCAACAAAGTGGATTCACGTAGATTCTTTCAGCAAAAACTTAGGAGACATTGGGAATTGTACTGATCTAAATGAAAGTTGTGAGAGATGGGCTGCCTTAGGGGAATGCACCAAAAACCCAGAATATATGGTTGGATCTCCAGAAATGCCCGGCTACTGTCGGAGGAGTTGCAGGATCTGTTGATTTCATTTCAACCTTACACTCGAAATTCCGATACCTTTTCGTGTCATTTGTGGAATGGAAAGCGATACAACATTGATTGTGAAGCTATCGTGGAAGCACAGATGGGATGAAGCAGTAGCCGTTGGGTAATTATGACCTTTCCTTTTTCTTTAAAAAAAATTCATGATTATGTATTACTTTATATTGTCGTTATTTTTCTTACTTGAAT

Coding sequence (CDS)

ATGTTCAAATTTGATAATCTGTTATTCATCTTCTTGATTTTGACCTCATCGTTTATTCGGGAATCAACTTGTTCTTATGCTGGTTCGGCTAGCGCAACCGTAGATCCTAGTAAAGTGAAGCAGATTTCATGGAAACCGAGAGCTTTTGTATATGAAGGTTTTCTAACGGACCTAGAATGCGACCATCTGGTTTCTATAGCGAGATCCGAGCTAAAGAGATCTGAGGTTGCTGATAATGATTCGGGAAAGAGTAAGCTCAGTACTGTACGAACGAGTTCAGGAATGTTCATTTCTAAAAACAAGGATCCTATTGTTTCTGGCATAGAGGACAAAATTTCTGCATGGACTTTTCTTCCAAAAGAAAATGGGGAGGACATTCAGGTATTGAGATACGAGCATGGGCAGAAATATGAGTCACATTATGATTACTTTGTTGACAAGGTGAATATTGCCTGGGGTGGACATCGTTTAGCTACGGTCCTTATGTATCTCTCTAATGTGACCCAAGGTGGTGAAACAGTTTTCCCCTTGGCAGAGAAACCTTCCCACCGGAGAGCTTATGAAACAGACGAGGACCTCTCAGAGTGCGCTAAGAAAGGAGTTGCAGTGAAACCAAAGAAAGGCGATGCCCTTCTTTTCTTTAGCCTTGAACCAAATGCTATACCAGACACAAACAGTCTCCATGGAGGTTGCCCTGTCCTTGAAGGAGAAAAATGGTCAGCAACAAAGTGGATTCACGTAGATTCTTTCAGCAAAAACTTAGGAGACATTGGGAATTGTACTGATCTAAATGAAAGTTGTGAGAGATGGGCTGCCTTAGGGGAATGCACCAAAAACCCAGAATATATGGTTGGATCTCCAGAAATGCCCGGCTACTGTCGGAGGAGTTGCAGGATCTGTTGA

Protein sequence

MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPKENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEKPSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC*

Homology

[Close]

BLAST of Cucsa.363400 vs. Swiss-Prot
Match: P4H4_ARATH (Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana GN=P4H4 PE=2 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 6.2e-128
Identity = 219/294 (74.49%), Postives = 250/294 (85.03%), Query Frame = 1


Query: 7   LLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSI 66
           LL  F  + S  ++ ST S   S+S  V+PSKVKQ+S KPRAFVYEGFLT+LECDH+VS+
Sbjct: 6   LLISFFAIFSVLLQSST-SLISSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHMVSL 65

Query: 67  ARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPKENGEDI 126
           A++ LKRS VADNDSG+SK S VRTSSG FISK KDPIVSGIEDKIS WTFLPKENGEDI
Sbjct: 66  AKASLKRSAVADNDSGESKFSEVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDI 125

Query: 127 QVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEKPSHRRA 186
           QVLRYEHGQKY++H+DYF DKVNI  GGHR+AT+LMYLSNVT+GGETVFP AE PS R  
Sbjct: 126 QVLRYEHGQKYDAHFDYFHDKVNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPSRRVL 185

Query: 187 YETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIH 246
            E  EDLS+CAK+G+AVKP+KGDALLFF+L P+AIPD  SLHGGCPV+EGEKWSATKWIH
Sbjct: 186 SENKEDLSDCAKRGIAVKPRKGDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATKWIH 245

Query: 247 VDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
           VDSF + +   GNCTD+NESCERWA LGECTKNPEYMVG+ E+PGYCRRSC+ C
Sbjct: 246 VDSFDRIVTPSGNCTDMNESCERWAVLGECTKNPEYMVGTTELPGYCRRSCKAC 298

[Close]

BLAST of Cucsa.363400 vs. Swiss-Prot
Match: P4H2_ARATH (Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana GN=P4H2 PE=1 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 8.3e-125
Identity = 214/300 (71.33%), Postives = 255/300 (85.00%), Query Frame = 1


Query: 1   MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           M +   LLF+ ++L    ++ STC  + S S+ ++PSKVKQ+S KPRAFVYEGFLTDLEC
Sbjct: 3   MSRLGLLLFVAILLV--LLQSSTCLIS-SPSSIINPSKVKQVSSKPRAFVYEGFLTDLEC 62

Query: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120
           DHL+S+A+  L+RS VADND+G+S++S VRTSSG FISK KDPIVSGIEDK+S WTFLPK
Sbjct: 63  DHLISLAKENLQRSAVADNDNGESQVSDVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPK 122

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEK 180
           ENGED+QVLRYEHGQKY++H+DYF DKVNIA GGHR+ATVL+YLSNVT+GGETVFP A++
Sbjct: 123 ENGEDLQVLRYEHGQKYDAHFDYFHDKVNIARGGHRIATVLLYLSNVTKGGETVFPDAQE 182

Query: 181 PSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240
            S R   E  +DLS+CAKKG+AVKPKKG+ALLFF+L+ +AIPD  SLHGGCPV+EGEKWS
Sbjct: 183 FSRRSLSENKDDLSDCAKKGIAVKPKKGNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWS 242

Query: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300
           ATKWIHVDSF K L   GNCTD+NESCERWA LGEC KNPEYMVG+PE+PG CRRSC+ C
Sbjct: 243 ATKWIHVDSFDKILTHDGNCTDVNESCERWAVLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

[Close]

BLAST of Cucsa.363400 vs. Swiss-Prot
Match: P4H7_ARATH (Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana GN=P4H7 PE=2 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 8.7e-98
Identity = 165/272 (60.66%), Postives = 209/272 (76.84%), Query Frame = 1


Query: 29  SASATVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSIARSELKRSEVADNDSGKSKLST 88
           ++S   DP++V Q+SW PR F+YEGFL+D ECDH + +A+ +L++S VADNDSG+S  S 
Sbjct: 46  ASSFGFDPTRVTQLSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESE 105

Query: 89  VRTSSGMFISKNKDPIVSGIEDKISAWTFLPKENGEDIQVLRYEHGQKYESHYDYFVDKV 148
           VRTSSGMF+SK +D IVS +E K++AWTFLP+ENGE +Q+L YE+GQKYE H+DYF D+ 
Sbjct: 106 VRTSSGMFLSKRQDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQA 165

Query: 149 NIAWGGHRLATVLMYLSNVTQGGETVFPLAEKPSHRRAYETDEDLSECAKKGVAVKPKKG 208
           N+  GGHR+ATVLMYLSNV +GGETVFP+ +    +     D+  +ECAK+G AVKP+KG
Sbjct: 166 NLELGGHRIATVLMYLSNVEKGGETVFPMWK---GKATQLKDDSWTECAKQGYAVKPRKG 225

Query: 209 DALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIHVDSFSKNLGDIGNCTDLNESCE 268
           DALLFF+L PNA  D+NSLHG CPV+EGEKWSAT+WIHV SF +       C D N SCE
Sbjct: 226 DALLFFNLHPNATTDSNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQSGCMDENVSCE 285

Query: 269 RWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
           +WA  GEC KNP YMVGS +  GYCR+SC+ C
Sbjct: 286 KWAKAGECQKNPTYMVGSDKDHGYCRKSCKAC 314

[Close]

BLAST of Cucsa.363400 vs. Swiss-Prot
Match: P4H6_ARATH (Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana GN=P4H6 PE=2 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 7.9e-91
Identity = 162/277 (58.48%), Postives = 207/277 (74.73%), Query Frame = 1


Query: 25  SYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSIARSELKRSEV-ADNDSGK 84
           S   S S +VDP+++ Q+SW PRAF+Y+GFL+D ECDHL+ +A+ +L++S V AD DSG+
Sbjct: 18  SQISSFSFSVDPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAKGKLEKSMVVADVDSGE 77

Query: 85  SKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPKENGEDIQVLRYEHGQKYESHYDY 144
           S+ S VRTSSGMF++K +D IV+ +E K++AWTFLP+ENGE +Q+L YE+GQKY+ H+DY
Sbjct: 78  SEDSEVRTSSGMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQILHYENGQKYDPHFDY 137

Query: 145 FVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEKPSHRRAYETDEDLSECAKKGVAV 204
           F DK  +  GGHR+ATVLMYLSNVT+GGETVFP       +     D+  S+CAK+G AV
Sbjct: 138 FYDKKALELGGHRIATVLMYLSNVTKGGETVFP---NWKGKTPQLKDDSWSKCAKQGYAV 197

Query: 205 KPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIHVDSFSKNLGDIGNCTDL 264
           KP+KGDALLFF+L  N   D NSLHG CPV+EGEKWSAT+WIHV SF K       C D 
Sbjct: 198 KPRKGDALLFFNLHLNGTTDPNSLHGSCPVIEGEKWSATRWIHVRSFGKKK---LVCVDD 257

Query: 265 NESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
           +ESC+ WA  GEC KNP YMVGS    G+CR+SC+ C
Sbjct: 258 HESCQEWADAGECEKNPMYMVGSETSLGFCRKSCKAC 288

[Close]

BLAST of Cucsa.363400 vs. Swiss-Prot
Match: P4H3_ARATH (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana GN=P4H3 PE=2 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 1.4e-63
Identity = 114/209 (54.55%), Postives = 156/209 (74.64%), Query Frame = 1


Query: 42  ISWKPRAFVYEGFLTDLECDHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNK 101
           +SW+PRAFVY  FL+  EC++L+S+A+  + +S V D+++GKSK S VRTSSG F+ + +
Sbjct: 79  LSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETGKSKDSRVRTSSGTFLRRGR 138

Query: 102 DPIVSGIEDKISAWTFLPKENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVL 161
           D I+  IE +I+ +TF+P ++GE +QVL YE GQKYE HYDYFVD+ N   GG R+AT+L
Sbjct: 139 DKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYDYFVDEFNTKNGGQRMATML 198

Query: 162 MYLSNVTQGGETVFPLAEKPSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAI 221
           MYLS+V +GGETVFP A    +  +     +LSEC KKG++VKP+ GDALLF+S+ P+A 
Sbjct: 199 MYLSDVEEGGETVFPAAN--MNFSSVPWYNELSECGKKGLSVKPRMGDALLFWSMRPDAT 258

Query: 222 PDTNSLHGGCPVLEGEKWSATKWIHVDSF 251
            D  SLHGGCPV+ G KWS+TKW+HV  +
Sbjct: 259 LDPTSLHGGCPVIRGNKWSSTKWMHVGEY 285

[Close]

BLAST of Cucsa.363400 vs. TrEMBL
Match: A0A0A0KCQ5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G067350 PE=4 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 4.7e-175
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 1


Query: 1   MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120
           DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEK 180

Query: 181 PSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240
           PSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS
Sbjct: 181 PSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300
           ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC
Sbjct: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300

[Close]

BLAST of Cucsa.363400 vs. TrEMBL
Match: B9RSW4_RICCO (Prolyl 4-hydroxylase alpha subunit, putative OS=Ricinus communis GN=RCOM_0679070 PE=4 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 2.8e-135
Identity = 226/292 (77.40%), Postives = 260/292 (89.04%), Query Frame = 1


Query: 9   FIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSIAR 68
           F+FL+L S    +S+ SY GS ++ +DPSKVKQ+SWKPRAFVYEGFLTDLECDHL+S+A+
Sbjct: 7   FVFLLLISLIFHKSS-SYPGSPTSIIDPSKVKQVSWKPRAFVYEGFLTDLECDHLISLAK 66

Query: 69  SELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPKENGEDIQV 128
           SELKRS VADN+SGKSKLS VRTSSGMFI+K KDPI++GIE+KIS WTFLPKENGED+QV
Sbjct: 67  SELKRSAVADNESGKSKLSEVRTSSGMFIAKGKDPIIAGIEEKISTWTFLPKENGEDLQV 126

Query: 129 LRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEKPSHRRAYE 188
           LRYEHGQKY+ HYDYF DK+NIA GGHR+ATVLMYLS+V +GGETVFP AE+P  R+A E
Sbjct: 127 LRYEHGQKYDPHYDYFADKINIARGGHRMATVLMYLSDVVKGGETVFPNAEEPPRRKATE 186

Query: 189 TDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIHVD 248
           + EDLSECAKKG++VKP++GDALLFFSL P AIPD NSLH GCPV+EGEKWSATKWIHVD
Sbjct: 187 SHEDLSECAKKGISVKPRRGDALLFFSLHPTAIPDPNSLHAGCPVIEGEKWSATKWIHVD 246

Query: 249 SFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
           SF KN+   GNCTD NESCERWAALGECT NPEYMVGSPE+PGYCRRSC++C
Sbjct: 247 SFDKNIEAGGNCTDKNESCERWAALGECTNNPEYMVGSPELPGYCRRSCKVC 297

[Close]

BLAST of Cucsa.363400 vs. TrEMBL
Match: W9SGN4_9ROSA (Prolyl 4-hydroxylase subunit alpha-1 OS=Morus notabilis GN=L484_011286 PE=4 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 2.3e-134
Identity = 228/293 (77.82%), Postives = 258/293 (88.05%), Query Frame = 1


Query: 8   LFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSIA 67
           LF+FL+  SS   ES+ SYAGSA++ ++PSKVKQ+SWKPRAFVYEGFLTDLECDHL+S+A
Sbjct: 8   LFLFLLSISSSFHESSSSYAGSAASIINPSKVKQVSWKPRAFVYEGFLTDLECDHLISLA 67

Query: 68  RSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPKENGEDIQ 127
           +SELKRS VADN SGKSKLS VRTSSGMFI K KDPIV+GIEDKIS WTFLPKENGED+Q
Sbjct: 68  KSELKRSAVADNVSGKSKLSEVRTSSGMFIPKAKDPIVAGIEDKISTWTFLPKENGEDMQ 127

Query: 128 VLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEKPSHRRAY 187
           VLRYEHGQKY+ HYDYF DKVNIA GGHR+ATVLMYL++V +GGETVFP AE+  H +A 
Sbjct: 128 VLRYEHGQKYDPHYDYFADKVNIARGGHRIATVLMYLTDVVKGGETVFPSAEESHHHKAS 187

Query: 188 ETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIHV 247
            TD+DLSECAKKG+AVKP++GDALLFFSL P A+PDT SLH GCPV+EGEKWSATKWIHV
Sbjct: 188 TTDDDLSECAKKGIAVKPRRGDALLFFSLLPTAVPDTISLHAGCPVIEGEKWSATKWIHV 247

Query: 248 DSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
           DSF K+L   G CTD NESCERWAALGEC KN EYMVGSPE+PGYCRRSC++C
Sbjct: 248 DSFDKDLSAGGKCTDQNESCERWAALGECNKNREYMVGSPELPGYCRRSCKVC 300

[Close]

BLAST of Cucsa.363400 vs. TrEMBL
Match: A0A067L7I1_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02052 PE=4 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 8.9e-134
Identity = 230/294 (78.23%), Postives = 260/294 (88.44%), Query Frame = 1


Query: 7   LLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSI 66
           L F+FL L+ S I   + SY G++S+ +DP+KVKQ+SWKPRAFVY GFLTDLECDHL+S+
Sbjct: 8   LQFLFL-LSISLILHKSGSYPGTSSSIIDPAKVKQVSWKPRAFVYHGFLTDLECDHLISL 67

Query: 67  ARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPKENGEDI 126
           A+SELKRS VADN SGKSK++ VRTSSGMFI K KDPIV+GIEDKI+ WTFLPKENGEDI
Sbjct: 68  AKSELKRSAVADNVSGKSKVAEVRTSSGMFIPKGKDPIVAGIEDKIATWTFLPKENGEDI 127

Query: 127 QVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEKPSHRRA 186
           QVLRYE+GQKY+ HYDYFVD+VNIA GGHRLATVLMYLSNV +GGETVFP AE    R+A
Sbjct: 128 QVLRYEYGQKYDPHYDYFVDRVNIARGGHRLATVLMYLSNVEKGGETVFPSAEDAPRRKA 187

Query: 187 YETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIH 246
            E DEDLSECAKKG+AVKP++GDALLFFSL PNA+PD +SLH GCPV+EGEKWSATKWIH
Sbjct: 188 NEGDEDLSECAKKGIAVKPRRGDALLFFSLLPNAVPDQSSLHAGCPVIEGEKWSATKWIH 247

Query: 247 VDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
           VDSFSKNL   GNCTDLNESCERWAALGECTKNPEYMVGS E+PGYCRRSC++C
Sbjct: 248 VDSFSKNLEADGNCTDLNESCERWAALGECTKNPEYMVGSAELPGYCRRSCKVC 300

[Close]

BLAST of Cucsa.363400 vs. TrEMBL
Match: A0A0B2P6V3_GLYSO (Prolyl 4-hydroxylase subunit alpha-2 OS=Glycine soja GN=glysoja_004434 PE=4 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 5.4e-131
Identity = 224/292 (76.71%), Postives = 255/292 (87.33%), Query Frame = 1


Query: 9   FIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSIAR 68
           F+  +L  S   +   SYAGSAS+ V+PSKVKQISWKPRAFVYEGFLTDLECDHL+S+A+
Sbjct: 7   FLLFLLLISKCHQVWGSYAGSASSIVNPSKVKQISWKPRAFVYEGFLTDLECDHLISLAK 66

Query: 69  SELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPKENGEDIQV 128
           SELKRS VADN SG+S+LS VRTSSGMFISKNKDPI+SGIEDKIS+WTFLPKENGEDIQV
Sbjct: 67  SELKRSAVADNLSGESQLSDVRTSSGMFISKNKDPIISGIEDKISSWTFLPKENGEDIQV 126

Query: 129 LRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEKPSHRRAYE 188
           LRYEHGQKY+ HYDYF DKVNIA GGHR+ATVLMYL+NVT+GGETVFP AE+P  RR  E
Sbjct: 127 LRYEHGQKYDPHYDYFTDKVNIARGGHRIATVLMYLTNVTKGGETVFPSAEEPPRRRGTE 186

Query: 189 TDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIHVD 248
           T  DLSECAKKG+AVKP +GDALLFFSL  NA PDT+SLH GCPV+EGEKWSATKWIHVD
Sbjct: 187 TSSDLSECAKKGIAVKPHRGDALLFFSLHTNATPDTSSLHAGCPVIEGEKWSATKWIHVD 246

Query: 249 SFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
           SF K +G  G+C+D + SCERWA+LGECTKNPEYM+GS ++PGYCR+SC+ C
Sbjct: 247 SFDKTVGAGGDCSDHHVSCERWASLGECTKNPEYMIGSSDVPGYCRKSCKSC 298

[Close]

BLAST of Cucsa.363400 vs. TAIR10
Match: AT5G18900.1 (AT5G18900.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 458.4 bits (1178), Expect = 3.5e-129
Identity = 219/294 (74.49%), Postives = 250/294 (85.03%), Query Frame = 1


Query: 7   LLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSI 66
           LL  F  + S  ++ ST S   S+S  V+PSKVKQ+S KPRAFVYEGFLT+LECDH+VS+
Sbjct: 6   LLISFFAIFSVLLQSST-SLISSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHMVSL 65

Query: 67  ARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPKENGEDI 126
           A++ LKRS VADNDSG+SK S VRTSSG FISK KDPIVSGIEDKIS WTFLPKENGEDI
Sbjct: 66  AKASLKRSAVADNDSGESKFSEVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDI 125

Query: 127 QVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEKPSHRRA 186
           QVLRYEHGQKY++H+DYF DKVNI  GGHR+AT+LMYLSNVT+GGETVFP AE PS R  
Sbjct: 126 QVLRYEHGQKYDAHFDYFHDKVNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPSRRVL 185

Query: 187 YETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIH 246
            E  EDLS+CAK+G+AVKP+KGDALLFF+L P+AIPD  SLHGGCPV+EGEKWSATKWIH
Sbjct: 186 SENKEDLSDCAKRGIAVKPRKGDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATKWIH 245

Query: 247 VDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
           VDSF + +   GNCTD+NESCERWA LGECTKNPEYMVG+ E+PGYCRRSC+ C
Sbjct: 246 VDSFDRIVTPSGNCTDMNESCERWAVLGECTKNPEYMVGTTELPGYCRRSCKAC 298

[Close]

BLAST of Cucsa.363400 vs. TAIR10
Match: AT3G06300.1 (AT3G06300.1 P4H isoform 2)

HSP 1 Score: 448.0 bits (1151), Expect = 4.7e-126
Identity = 214/300 (71.33%), Postives = 255/300 (85.00%), Query Frame = 1


Query: 1   MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           M +   LLF+ ++L    ++ STC  + S S+ ++PSKVKQ+S KPRAFVYEGFLTDLEC
Sbjct: 3   MSRLGLLLFVAILLV--LLQSSTCLIS-SPSSIINPSKVKQVSSKPRAFVYEGFLTDLEC 62

Query: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120
           DHL+S+A+  L+RS VADND+G+S++S VRTSSG FISK KDPIVSGIEDK+S WTFLPK
Sbjct: 63  DHLISLAKENLQRSAVADNDNGESQVSDVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPK 122

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEK 180
           ENGED+QVLRYEHGQKY++H+DYF DKVNIA GGHR+ATVL+YLSNVT+GGETVFP A++
Sbjct: 123 ENGEDLQVLRYEHGQKYDAHFDYFHDKVNIARGGHRIATVLLYLSNVTKGGETVFPDAQE 182

Query: 181 PSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240
            S R   E  +DLS+CAKKG+AVKPKKG+ALLFF+L+ +AIPD  SLHGGCPV+EGEKWS
Sbjct: 183 FSRRSLSENKDDLSDCAKKGIAVKPKKGNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWS 242

Query: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300
           ATKWIHVDSF K L   GNCTD+NESCERWA LGEC KNPEYMVG+PE+PG CRRSC+ C
Sbjct: 243 ATKWIHVDSFDKILTHDGNCTDVNESCERWAVLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

[Close]

BLAST of Cucsa.363400 vs. TAIR10
Match: AT3G28480.2 (AT3G28480.2 Oxoglutarate/iron-dependent oxygenase)

HSP 1 Score: 335.9 bits (860), Expect = 2.6e-92
Identity = 159/280 (56.79%), Postives = 205/280 (73.21%), Query Frame = 1


Query: 29  SASATVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSIARSELKRSEVADNDSGKS---- 88
           ++S   DP++V Q+SW PR F+YEGFL+D ECDH + +A+ +L++S VADNDSG+S    
Sbjct: 46  ASSFGFDPTRVTQLSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESE 105

Query: 89  -KLSTVRTSSGMFISKNK---DPIVSGIEDKISAWTFLPKENGEDIQVLRYEHGQKYESH 148
             +S VR SS    + +    D IVS +E K++AWTFLP+ENGE +Q+L YE+GQKYE H
Sbjct: 106 DSVSVVRQSSSFIANMDSLEIDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPH 165

Query: 149 YDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEKPSHRRAYETDEDLSECAKKG 208
           +DYF D+ N+  GGHR+ATVLMYLSNV +GGETVFP+ +  + +     D+  +ECAK+G
Sbjct: 166 FDYFHDQANLELGGHRIATVLMYLSNVEKGGETVFPMWKGKATQLK---DDSWTECAKQG 225

Query: 209 VAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIHVDSFSKNLGDIGNC 268
            AVKP+KGDALLFF+L PNA  D+NSLHG CPV+EGEKWSAT+WIHV SF +       C
Sbjct: 226 YAVKPRKGDALLFFNLHPNATTDSNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQSGC 285

Query: 269 TDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
            D N SCE+WA  GEC KNP YMVGS +  GYCR+SC+ C
Sbjct: 286 MDENVSCEKWAKAGECQKNPTYMVGSDKDHGYCRKSCKAC 322

[Close]

BLAST of Cucsa.363400 vs. TAIR10
Match: AT3G28490.1 (AT3G28490.1 Oxoglutarate/iron-dependent oxygenase)

HSP 1 Score: 335.1 bits (858), Expect = 4.4e-92
Identity = 162/277 (58.48%), Postives = 207/277 (74.73%), Query Frame = 1


Query: 25  SYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSIARSELKRSEV-ADNDSGK 84
           S   S S +VDP+++ Q+SW PRAF+Y+GFL+D ECDHL+ +A+ +L++S V AD DSG+
Sbjct: 18  SQISSFSFSVDPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAKGKLEKSMVVADVDSGE 77

Query: 85  SKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPKENGEDIQVLRYEHGQKYESHYDY 144
           S+ S VRTSSGMF++K +D IV+ +E K++AWTFLP+ENGE +Q+L YE+GQKY+ H+DY
Sbjct: 78  SEDSEVRTSSGMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQILHYENGQKYDPHFDY 137

Query: 145 FVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEKPSHRRAYETDEDLSECAKKGVAV 204
           F DK  +  GGHR+ATVLMYLSNVT+GGETVFP       +     D+  S+CAK+G AV
Sbjct: 138 FYDKKALELGGHRIATVLMYLSNVTKGGETVFP---NWKGKTPQLKDDSWSKCAKQGYAV 197

Query: 205 KPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIHVDSFSKNLGDIGNCTDL 264
           KP+KGDALLFF+L  N   D NSLHG CPV+EGEKWSAT+WIHV SF K       C D 
Sbjct: 198 KPRKGDALLFFNLHLNGTTDPNSLHGSCPVIEGEKWSATRWIHVRSFGKKK---LVCVDD 257

Query: 265 NESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
           +ESC+ WA  GEC KNP YMVGS    G+CR+SC+ C
Sbjct: 258 HESCQEWADAGECEKNPMYMVGSETSLGFCRKSCKAC 288

[Close]

BLAST of Cucsa.363400 vs. TAIR10
Match: AT1G20270.1 (AT1G20270.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 244.6 bits (623), Expect = 7.9e-65
Identity = 114/209 (54.55%), Postives = 156/209 (74.64%), Query Frame = 1


Query: 42  ISWKPRAFVYEGFLTDLECDHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNK 101
           +SW+PRAFVY  FL+  EC++L+S+A+  + +S V D+++GKSK S VRTSSG F+ + +
Sbjct: 79  LSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETGKSKDSRVRTSSGTFLRRGR 138

Query: 102 DPIVSGIEDKISAWTFLPKENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVL 161
           D I+  IE +I+ +TF+P ++GE +QVL YE GQKYE HYDYFVD+ N   GG R+AT+L
Sbjct: 139 DKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYDYFVDEFNTKNGGQRMATML 198

Query: 162 MYLSNVTQGGETVFPLAEKPSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAI 221
           MYLS+V +GGETVFP A    +  +     +LSEC KKG++VKP+ GDALLF+S+ P+A 
Sbjct: 199 MYLSDVEEGGETVFPAAN--MNFSSVPWYNELSECGKKGLSVKPRMGDALLFWSMRPDAT 258

Query: 222 PDTNSLHGGCPVLEGEKWSATKWIHVDSF 251
            D  SLHGGCPV+ G KWS+TKW+HV  +
Sbjct: 259 LDPTSLHGGCPVIRGNKWSSTKWMHVGEY 285

[Close]

BLAST of Cucsa.363400 vs. NCBI nr
Match: gi|449454448|ref|XP_004144967.1| (PREDICTED: probable prolyl 4-hydroxylase 4 [Cucumis sativus])

HSP 1 Score: 621.7 bits (1602), Expect = 6.7e-175
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 1


Query: 1   MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120
           DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEK 180

Query: 181 PSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240
           PSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS
Sbjct: 181 PSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300
           ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC
Sbjct: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300

[Close]

BLAST of Cucsa.363400 vs. NCBI nr
Match: gi|659117281|ref|XP_008458517.1| (PREDICTED: prolyl 4-hydroxylase subunit alpha-1-like [Cucumis melo])

HSP 1 Score: 608.2 bits (1567), Expect = 7.7e-171
Identity = 292/300 (97.33%), Postives = 296/300 (98.67%), Query Frame = 1


Query: 1   MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           MFKF NLLF FLIL SSF+RESTCSYAGSASATVDPS+VKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MFKFRNLLFFFLILISSFVRESTCSYAGSASATVDPSRVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120
           DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVT+GGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTKGGETVFPLAEK 180

Query: 181 PSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240
            SHRRAYETDEDLSECAKKG+AVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS
Sbjct: 181 SSHRRAYETDEDLSECAKKGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300
           ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC
Sbjct: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300

[Close]

BLAST of Cucsa.363400 vs. NCBI nr
Match: gi|255551575|ref|XP_002516833.1| (PREDICTED: probable prolyl 4-hydroxylase 4 [Ricinus communis])

HSP 1 Score: 489.6 bits (1259), Expect = 4.0e-135
Identity = 226/292 (77.40%), Postives = 260/292 (89.04%), Query Frame = 1


Query: 9   FIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSIAR 68
           F+FL+L S    +S+ SY GS ++ +DPSKVKQ+SWKPRAFVYEGFLTDLECDHL+S+A+
Sbjct: 7   FVFLLLISLIFHKSS-SYPGSPTSIIDPSKVKQVSWKPRAFVYEGFLTDLECDHLISLAK 66

Query: 69  SELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPKENGEDIQV 128
           SELKRS VADN+SGKSKLS VRTSSGMFI+K KDPI++GIE+KIS WTFLPKENGED+QV
Sbjct: 67  SELKRSAVADNESGKSKLSEVRTSSGMFIAKGKDPIIAGIEEKISTWTFLPKENGEDLQV 126

Query: 129 LRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEKPSHRRAYE 188
           LRYEHGQKY+ HYDYF DK+NIA GGHR+ATVLMYLS+V +GGETVFP AE+P  R+A E
Sbjct: 127 LRYEHGQKYDPHYDYFADKINIARGGHRMATVLMYLSDVVKGGETVFPNAEEPPRRKATE 186

Query: 189 TDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIHVD 248
           + EDLSECAKKG++VKP++GDALLFFSL P AIPD NSLH GCPV+EGEKWSATKWIHVD
Sbjct: 187 SHEDLSECAKKGISVKPRRGDALLFFSLHPTAIPDPNSLHAGCPVIEGEKWSATKWIHVD 246

Query: 249 SFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
           SF KN+   GNCTD NESCERWAALGECT NPEYMVGSPE+PGYCRRSC++C
Sbjct: 247 SFDKNIEAGGNCTDKNESCERWAALGECTNNPEYMVGSPELPGYCRRSCKVC 297

[Close]

BLAST of Cucsa.363400 vs. NCBI nr
Match: gi|703134588|ref|XP_010105675.1| (Prolyl 4-hydroxylase subunit alpha-1 [Morus notabilis])

HSP 1 Score: 486.5 bits (1251), Expect = 3.4e-134
Identity = 228/293 (77.82%), Postives = 258/293 (88.05%), Query Frame = 1


Query: 8   LFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSIA 67
           LF+FL+  SS   ES+ SYAGSA++ ++PSKVKQ+SWKPRAFVYEGFLTDLECDHL+S+A
Sbjct: 8   LFLFLLSISSSFHESSSSYAGSAASIINPSKVKQVSWKPRAFVYEGFLTDLECDHLISLA 67

Query: 68  RSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPKENGEDIQ 127
           +SELKRS VADN SGKSKLS VRTSSGMFI K KDPIV+GIEDKIS WTFLPKENGED+Q
Sbjct: 68  KSELKRSAVADNVSGKSKLSEVRTSSGMFIPKAKDPIVAGIEDKISTWTFLPKENGEDMQ 127

Query: 128 VLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEKPSHRRAY 187
           VLRYEHGQKY+ HYDYF DKVNIA GGHR+ATVLMYL++V +GGETVFP AE+  H +A 
Sbjct: 128 VLRYEHGQKYDPHYDYFADKVNIARGGHRIATVLMYLTDVVKGGETVFPSAEESHHHKAS 187

Query: 188 ETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIHV 247
            TD+DLSECAKKG+AVKP++GDALLFFSL P A+PDT SLH GCPV+EGEKWSATKWIHV
Sbjct: 188 TTDDDLSECAKKGIAVKPRRGDALLFFSLLPTAVPDTISLHAGCPVIEGEKWSATKWIHV 247

Query: 248 DSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
           DSF K+L   G CTD NESCERWAALGEC KN EYMVGSPE+PGYCRRSC++C
Sbjct: 248 DSFDKDLSAGGKCTDQNESCERWAALGECNKNREYMVGSPELPGYCRRSCKVC 300

[Close]

BLAST of Cucsa.363400 vs. NCBI nr
Match: gi|802578382|ref|XP_012069451.1| (PREDICTED: probable prolyl 4-hydroxylase 4 [Jatropha curcas])

HSP 1 Score: 484.6 bits (1246), Expect = 1.3e-133
Identity = 230/294 (78.23%), Postives = 260/294 (88.44%), Query Frame = 1


Query: 7   LLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSI 66
           L F+FL L+ S I   + SY G++S+ +DP+KVKQ+SWKPRAFVY GFLTDLECDHL+S+
Sbjct: 8   LQFLFL-LSISLILHKSGSYPGTSSSIIDPAKVKQVSWKPRAFVYHGFLTDLECDHLISL 67

Query: 67  ARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPKENGEDI 126
           A+SELKRS VADN SGKSK++ VRTSSGMFI K KDPIV+GIEDKI+ WTFLPKENGEDI
Sbjct: 68  AKSELKRSAVADNVSGKSKVAEVRTSSGMFIPKGKDPIVAGIEDKIATWTFLPKENGEDI 127

Query: 127 QVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEKPSHRRA 186
           QVLRYE+GQKY+ HYDYFVD+VNIA GGHRLATVLMYLSNV +GGETVFP AE    R+A
Sbjct: 128 QVLRYEYGQKYDPHYDYFVDRVNIARGGHRLATVLMYLSNVEKGGETVFPSAEDAPRRKA 187

Query: 187 YETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIH 246
            E DEDLSECAKKG+AVKP++GDALLFFSL PNA+PD +SLH GCPV+EGEKWSATKWIH
Sbjct: 188 NEGDEDLSECAKKGIAVKPRRGDALLFFSLLPNAVPDQSSLHAGCPVIEGEKWSATKWIH 247

Query: 247 VDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
           VDSFSKNL   GNCTDLNESCERWAALGECTKNPEYMVGS E+PGYCRRSC++C
Sbjct: 248 VDSFSKNLEADGNCTDLNESCERWAALGECTKNPEYMVGSAELPGYCRRSCKVC 300

The following BLAST results are available for this feature:

BLAST of Cucsa.363400 vs. Swiss-Prot


Match Name	E-value	Identity	Description
P4H4_ARATH	6.2e-128	74.49	Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana GN=P4H4 PE=2 SV=1	[more]
P4H2_ARATH	8.3e-125	71.33	Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana GN=P4H2 PE=1 SV=1	[more]
P4H7_ARATH	8.7e-98	60.66	Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana GN=P4H7 PE=2 SV=1	[more]
P4H6_ARATH	7.9e-91	58.48	Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana GN=P4H6 PE=2 SV=1	[more]
P4H3_ARATH	1.4e-63	54.55	Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana GN=P4H3 PE=2 SV=1	[more]

BLAST of Cucsa.363400 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KCQ5_CUCSA	4.7e-175	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G067350 PE=4 SV=1	[more]
B9RSW4_RICCO	2.8e-135	77.40	Prolyl 4-hydroxylase alpha subunit, putative OS=Ricinus communis GN=RCOM_0679070...	[more]
W9SGN4_9ROSA	2.3e-134	77.82	Prolyl 4-hydroxylase subunit alpha-1 OS=Morus notabilis GN=L484_011286 PE=4 SV=1	[more]
A0A067L7I1_JATCU	8.9e-134	78.23	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02052 PE=4 SV=1	[more]
A0A0B2P6V3_GLYSO	5.4e-131	76.71	Prolyl 4-hydroxylase subunit alpha-2 OS=Glycine soja GN=glysoja_004434 PE=4 SV=1	[more]

BLAST of Cucsa.363400 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G18900.1	3.5e-129	74.49	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT3G06300.1	4.7e-126	71.33	P4H isoform 2	[more]
AT3G28480.2	2.6e-92	56.79	Oxoglutarate/iron-dependent oxygenase	[more]
AT3G28490.1	4.4e-92	58.48	Oxoglutarate/iron-dependent oxygenase	[more]
AT1G20270.1	7.9e-65	54.55	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]

BLAST of Cucsa.363400 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449454448\|ref\|XP_004144967.1\|	6.7e-175	100.00	PREDICTED: probable prolyl 4-hydroxylase 4 [Cucumis sativus]	[more]
gi\|659117281\|ref\|XP_008458517.1\|	7.7e-171	97.33	PREDICTED: prolyl 4-hydroxylase subunit alpha-1-like [Cucumis melo]	[more]
gi\|255551575\|ref\|XP_002516833.1\|	4.0e-135	77.40	PREDICTED: probable prolyl 4-hydroxylase 4 [Ricinus communis]	[more]
gi\|703134588\|ref\|XP_010105675.1\|	3.4e-134	77.82	Prolyl 4-hydroxylase subunit alpha-1 [Morus notabilis]	[more]
gi\|802578382\|ref\|XP_012069451.1\|	1.3e-133	78.23	PREDICTED: probable prolyl 4-hydroxylase 4 [Jatropha curcas]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR003582	ShKT_dom
IPR005123	Oxoglu/Fe-dep_dioxygenase
IPR006620	Pro_4_hyd_alph

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0005506	iron ion binding
GO:0031418	L-ascorbic acid binding

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006525	arginine metabolic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0018401	peptidyl-proline hydroxylation to 4-hydroxy-L-proline
biological_process	GO:0006560	proline metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0031418	L-ascorbic acid binding
molecular_function	GO:0004656	procollagen-proline 4-dioxygenase activity
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.363400.1	Cucsa.363400.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003582	ShKT domain	PFAM	PF01549	ShK	coord: 259..300 score: 2.
IPR003582	ShKT domain	SMART	SM00254	ShkT_1	coord: 259..300 score: 0.
IPR003582	ShKT domain	PROFILE	PS51670	SHKT	coord: 260..300 score: 8
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PFAM	PF13640	2OG-FeII_Oxy_3	coord: 126..246 score: 4.4
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROFILE	PS51471	FE2OG_OXY	coord: 122..247 score: 12
IPR006620	Prolyl 4-hydroxylase, alpha subunit	SMART	SM00702	p4hc	coord: 46..246 score: 1.9
None	No IPR available	PANTHER	PTHR10869	PROLYL 4-HYDROXYLASE ALPHA SUBUNIT	coord: 25..282 score: 2.7E
None	No IPR available	PANTHER	PTHR10869:SF64	OXIDOREDUCTASE, 2OG-FE(II) OXYGENASE FAMILY PROTEIN-RELATED	coord: 25..282 score: 2.7E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.363400	Cla021690	Watermelon (97103) v1	cgywmB664
Cucsa.363400	Cla009353	Watermelon (97103) v1	cgywmB665
Cucsa.363400	Csa3G150820	Cucumber (Chinese Long) v2	cgycuB510
Cucsa.363400	Csa6G067350	Cucumber (Chinese Long) v2	cgycuB511
Cucsa.363400	MELO3C021380	Melon (DHL92) v3.5.1	cgymeB587
Cucsa.363400	MELO3C006592	Melon (DHL92) v3.5.1	cgymeB595
Cucsa.363400	ClCG06G005580	Watermelon (Charleston Gray)	cgywcgB619
Cucsa.363400	ClCG05G005650	Watermelon (Charleston Gray)	cgywcgB618
Cucsa.363400	CSPI03G13780	Wild cucumber (PI 183967)	cgycpiB538
Cucsa.363400	CSPI06G05250	Wild cucumber (PI 183967)	cgycpiB540
Cucsa.363400	CmaCh14G016540	Cucurbita maxima (Rimu)	cgycmaB0991
Cucsa.363400	CmaCh17G002910	Cucurbita maxima (Rimu)	cgycmaB0999
Cucsa.363400	CmaCh08G011910	Cucurbita maxima (Rimu)	cgycmaB1014
Cucsa.363400	CmoCh08G011630	Cucurbita moschata (Rifu)	cgycmoB1014
Cucsa.363400	CmoCh14G017000	Cucurbita moschata (Rifu)	cgycmoB0993
Cucsa.363400	CmoCh17G002790	Cucurbita moschata (Rifu)	cgycmoB1000
Cucsa.363400	Lsi05G014860	Bottle gourd (USVL1VR-Ls)	cgylsiB586
Cucsa.363400	Cp4.1LG12g02390	Cucurbita pepo (Zucchini)	cgycpeB0948
Cucsa.363400	Cp4.1LG17g01080	Cucurbita pepo (Zucchini)	cgycpeB0955
Cucsa.363400	Cp4.1LG03g11130	Cucurbita pepo (Zucchini)	cgycpeB0966
Cucsa.363400	MELO3C006592.2	Melon (DHL92) v3.6.1	cgymedB593
Cucsa.363400	MELO3C021380.2	Melon (DHL92) v3.6.1	cgymedB587
Cucsa.363400	CsaV3_6G005580	Cucumber (Chinese Long) v3	cgycucB556
Cucsa.363400	CsaV3_3G014000	Cucumber (Chinese Long) v3	cgycucB554
Cucsa.363400	Cla97C06G115090	Watermelon (97103) v2	cgywmbB624
Cucsa.363400	Cla97C05G087090	Watermelon (97103) v2	cgywmbB623
Cucsa.363400	Bhi12G001572	Wax gourd	cgywgoB725
Cucsa.363400	Carg17382	Silver-seed gourd	carcgyB0682
Cucsa.363400	Carg15947	Silver-seed gourd	carcgyB0384
Cucsa.363400	Carg05473	Silver-seed gourd	carcgyB0085

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Cucsa.363400	Cucsa.253720	Cucumber (Gy14) v1	cgycgyB131

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.363400	Bottle gourd (USVL1VR-Ls)	cgylsiB584
Cucsa.363400	Wax gourd	cgywgoB730