Cucsa.362300 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.362300
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Plant invertase/pectin methylesterase inhibitor
Location	scaffold03611 : 217704 .. 218343 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTTTACTCATGCAAAGAAAGAATAATTAAGATCATCATATGAAGCCCAAATTCTCCCCTTTCATGACCACTCCTCTCCCTTCTCTCCTCCTCTTCATAATTATCTTCAACATCTCTACCACATTTGTTGTTGCTGATCTTGTCCAAAAAGCTTGTAAAAAATGTGAAATTGACGATCCAAACATCAACTACAACTTCTGCACTTCATCTTTTCGTGCTCACTCGGGAAGTGATTCGACCGATCTTCGAAAACTCGGTGCTATCTCATTGAGTTTGATTCAAAGAAATTTAAGCAGCTCATTTGAGTATGTGGAAAAGCTATTACAAAACAAAGAAATAGATTCTTATAAAAGGGTTCGTTTAAATGATTGTCTAGATGTCTATTCGGATGCCATTGTTACTGTGGAAGAAGGAAAGAAAGCATATAAAGAAAAACATTATGATGATGCTAACATCAAAGTGAGCGCAGTGATGGATTCTGCTAGGGTTTGTGAAGATGGGTTTAGGGAAAAAGAAGGTGTCTCCTCTCCATTGACAAAGTGGAATAAGGACATGTTTCAATTGGCTGCCATAGCTCTTTCAATCATCAATATGCATCCGTAAAAGACAATAAACAATTCAAGGTGACTGCCACACCAT

mRNA sequence

AATTTTACTCATGCAAAGAAAGAATAATTAAGATCATCATATGAAGCCCAAATTCTCCCCTTTCATGACCACTCCTCTCCCTTCTCTCCTCCTCTTCATAATTATCTTCAACATCTCTACCACATTTGTTGTTGCTGATCTTGTCCAAAAAGCTTGTAAAAAATGTGAAATTGACGATCCAAACATCAACTACAACTTCTGCACTTCATCTTTTCGTGCTCACTCGGGAAGTGATTCGACCGATCTTCGAAAACTCGGTGCTATCTCATTGAGTTTGATTCAAAGAAATTTAAGCAGCTCATTTGAGTATGTGGAAAAGCTATTACAAAACAAAGAAATAGATTCTTATAAAAGGGTTCGTTTAAATGATTGTCTAGATGTCTATTCGGATGCCATTGTTACTGTGGAAGAAGGAAAGAAAGCATATAAAGAAAAACATTATGATGATGCTAACATCAAAGTGAGCGCAGTGATGGATTCTGCTAGGGTTTGTGAAGATGGGTTTAGGGAAAAAGAAGGTGTCTCCTCTCCATTGACAAAGTGGAATAAGGACATGTTTCAATTGGCTGCCATAGCTCTTTCAATCATCAATATGCATCCGTAAAAGACAATAAACAATTCAAGGTGACTGCCACACCAT

Coding sequence (CDS)

ATGAAGCCCAAATTCTCCCCTTTCATGACCACTCCTCTCCCTTCTCTCCTCCTCTTCATAATTATCTTCAACATCTCTACCACATTTGTTGTTGCTGATCTTGTCCAAAAAGCTTGTAAAAAATGTGAAATTGACGATCCAAACATCAACTACAACTTCTGCACTTCATCTTTTCGTGCTCACTCGGGAAGTGATTCGACCGATCTTCGAAAACTCGGTGCTATCTCATTGAGTTTGATTCAAAGAAATTTAAGCAGCTCATTTGAGTATGTGGAAAAGCTATTACAAAACAAAGAAATAGATTCTTATAAAAGGGTTCGTTTAAATGATTGTCTAGATGTCTATTCGGATGCCATTGTTACTGTGGAAGAAGGAAAGAAAGCATATAAAGAAAAACATTATGATGATGCTAACATCAAAGTGAGCGCAGTGATGGATTCTGCTAGGGTTTGTGAAGATGGGTTTAGGGAAAAAGAAGGTGTCTCCTCTCCATTGACAAAGTGGAATAAGGACATGTTTCAATTGGCTGCCATAGCTCTTTCAATCATCAATATGCATCCGTAA

Protein sequence

MKPKFSPFMTTPLPSLLLFIIIFNISTTFVVADLVQKACKKCEIDDPNINYNFCTSSFRAHSGSDSTDLRKLGAISLSLIQRNLSSSFEYVEKLLQNKEIDSYKRVRLNDCLDVYSDAIVTVEEGKKAYKEKHYDDANIKVSAVMDSARVCEDGFREKEGVSSPLTKWNKDMFQLAAIALSIINMHP*

Homology

[Close]

BLAST of Cucsa.362300 vs. Swiss-Prot
Match: PLA1_PLAAC (Putative invertase inhibitor OS=Platanus acerifolia PE=1 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 1.4e-29
Identity = 71/174 (40.80%), Postives = 106/174 (60.92%), Query Frame = 1


Query: 15  SLLLFIIIFNISTTF---VVADLVQKACKKCEIDDPNINYNFCTSSFRAHSGSDSTDLRK 74
           S  L I  FN+       + AD+VQ  CKK     PN+NY+FC  S  A   S + DL+ 
Sbjct: 4   SFSLCIFFFNLLLLLQAVISADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHTADLQG 63

Query: 75  LGAISLSLIQRNLSSSFEYVEKLLQNKEIDSYKRVRLNDCLDVYSDAIVTVEEGKKAYKE 134
           LG IS +L  ++ S    ++ ++L++K +D   +  LNDC+ +Y+DA  +V+E    +K 
Sbjct: 64  LGVISANLAIQHGSKIQTFIGRILKSK-VDPALKKYLNDCVGLYADAKSSVQEAIADFKS 123

Query: 135 KHYDDANIKVSAVMDSARVCEDGFREKEGVSSPLTKWNKDMFQLAAIALSIINM 186
           K Y  AN+K+SA +D +  CEDGF+EK+G+ SP+TK NKD  QL AI+L+I  +
Sbjct: 124 KDYASANVKMSAALDDSVTCEDGFKEKKGIVSPVTKENKDYVQLTAISLAITKL 176

[Close]

BLAST of Cucsa.362300 vs. TrEMBL
Match: A0A0A0K9X7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G080360 PE=4 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 3.1e-100
Identity = 186/187 (99.47%), Postives = 186/187 (99.47%), Query Frame = 1


Query: 1   MKPKFSPFMTTPLPSLLLFIIIFNISTTFVVADLVQKACKKCEIDDPNINYNFCTSSFRA 60
           MKPKFSPFMTTPLP LLLFIIIFNISTTFVVADLVQKACKKCEIDDPNINYNFCTSSFRA
Sbjct: 1   MKPKFSPFMTTPLPCLLLFIIIFNISTTFVVADLVQKACKKCEIDDPNINYNFCTSSFRA 60

Query: 61  HSGSDSTDLRKLGAISLSLIQRNLSSSFEYVEKLLQNKEIDSYKRVRLNDCLDVYSDAIV 120
           HSGSDSTDLRKLGAISLSLIQRNLSSSFEYVEKLLQNKEIDSYKRVRLNDCLDVYSDAIV
Sbjct: 61  HSGSDSTDLRKLGAISLSLIQRNLSSSFEYVEKLLQNKEIDSYKRVRLNDCLDVYSDAIV 120

Query: 121 TVEEGKKAYKEKHYDDANIKVSAVMDSARVCEDGFREKEGVSSPLTKWNKDMFQLAAIAL 180
           TVEEGKKAYKEKHYDDANIKVSAVMDSARVCEDGFREKEGVSSPLTKWNKDMFQLAAIAL
Sbjct: 121 TVEEGKKAYKEKHYDDANIKVSAVMDSARVCEDGFREKEGVSSPLTKWNKDMFQLAAIAL 180

Query: 181 SIINMHP 188
           SIINMHP
Sbjct: 181 SIINMHP 187

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BLAST of Cucsa.362300 vs. TrEMBL
Match: A0A061DWX5_THECC (Plant invertase/pectin methylesterase inhibitor superfamily protein OS=Theobroma cacao GN=TCM_005798 PE=4 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 1.3e-42
Identity = 93/182 (51.10%), Postives = 131/182 (71.98%), Query Frame = 1


Query: 9   MTTPLPSLLLFIIIFNISTTF---VVADLVQKACKKCEIDDPNINYNFCTSSFRAHSGSD 68
           M +  P L LF+  F  ++TF      DL+++ CKKC   DPN++YNFC +S +A   S 
Sbjct: 4   MLSSFPFLDLFLFFFIFTSTFHAVTSTDLIRETCKKCAGRDPNLSYNFCVTSLQAAPKSH 63

Query: 69  -STDLRKLGAISLSLIQRNLSSSFEYVEKLLQNKE-IDSYKRVRLNDCLDVYSDAIVTVE 128
            + DLR LG +S+ L++RNL+++  YVE+LL+NK+ +D + R  L+DC ++YSDAI + +
Sbjct: 64  CADDLRDLGMVSIRLVRRNLTNTRSYVEELLKNKKLVDPFVRSCLHDCFNLYSDAIPSTK 123

Query: 129 EGKKAYKEKHYDDANIKVSAVMDSARVCEDGFREKEGVSSPLTKWNKDMFQLAAIALSII 186
           E  + YK +HYDDANI VSAVMD+A  CEDGF+EKEGV SPLTK N + FQL+AI++SI+
Sbjct: 124 EAIEDYKSQHYDDANIDVSAVMDAATTCEDGFKEKEGVVSPLTKRNNNTFQLSAISISIV 183

[Close]

BLAST of Cucsa.362300 vs. TrEMBL
Match: B9MVC0_POPTR (Invertase/pectin methylesterase inhibitor family protein OS=Populus trichocarpa GN=POPTR_0014s04360g PE=4 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 6.5e-42
Identity = 87/171 (50.88%), Postives = 125/171 (73.10%), Query Frame = 1


Query: 16  LLLFI-IIFNISTTFVVADLVQKACKKCEIDDPNINYNFCTSSFRAHSGSDSTDLRKLGA 75
           LLLF+ ++F      +  +L+ + CKKC  +DPN++YNFC +S +A + S   +LR LG 
Sbjct: 9   LLLFLPLLFCTFHANIAQNLIPETCKKCAANDPNLSYNFCVTSLQASNRSQCDNLRGLGM 68

Query: 76  ISLSLIQRNLSSSFEYVEKLLQNKEIDSYKRVRLNDCLDVYSDAIVTVEEGKKAYKEKHY 135
           +S+ LI+ N++++  YV+ LL+NK++D + R  LNDCLD+YSDAI T+++    YK KHY
Sbjct: 69  MSIKLIKYNVTNTRHYVKNLLKNKKMDPFIRACLNDCLDLYSDAIPTLKQAMIDYKSKHY 128

Query: 136 DDANIKVSAVMDSARVCEDGFREKEGVSSPLTKWNKDMFQLAAIALSIINM 186
            DANI+VS+V+D+A  CEDGF +KEG  SPLTK N D FQL+AIAL++INM
Sbjct: 129 KDANIEVSSVIDAATTCEDGFEDKEGAVSPLTKRNNDTFQLSAIALALINM 179

[Close]

BLAST of Cucsa.362300 vs. TrEMBL
Match: A0A0D2PD74_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_004G168200 PE=4 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 4.7e-40
Identity = 92/191 (48.17%), Postives = 131/191 (68.59%), Query Frame = 1


Query: 1   MKPKFSPFMTTPLPSLLLFIIIFNISTTFVVA----DLVQKACKKCEIDDPNINYNFCTS 60
           M+   S     PL  L +F    +IST+  +      L++K CKKC   DPN++YNFC +
Sbjct: 1   MRQTLSSSFVIPL-FLCIFFFFVSISTSHGLTATNDSLIRKTCKKCAQSDPNLSYNFCIT 60

Query: 61  SFRAHSGSD-STDLRKLGAISLSLIQRNLSSSFEYVEKLLQN-KEIDSYKRVRLNDCLDV 120
           S +A   S  + DLR+LG IS++L+ RN++++  ++++LL+N K++D + R  L+DC D+
Sbjct: 61  SLQAAPHSHCANDLRQLGKISITLLGRNVTNTRSHIKELLKNQKKMDPFVRSCLHDCFDL 120

Query: 121 YSDAIVTVEEGKKAYKEKHYDDANIKVSAVMDSARVCEDGFREKEGVSSPLTKWNKDMFQ 180
           YSDAI T ++  + YK KHYDDANI VS+VMD+   CEDGF+EKEG+ SPLTK N D F 
Sbjct: 121 YSDAIPTTKQALQDYKAKHYDDANIDVSSVMDATTTCEDGFKEKEGLVSPLTKRNNDAFM 180

Query: 181 LAAIALSIINM 186
           L+AI+LSIINM
Sbjct: 181 LSAISLSIINM 190

[Close]

BLAST of Cucsa.362300 vs. TrEMBL
Match: M5X7H8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppb019226mg PE=4 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 1.4e-39
Identity = 81/180 (45.00%), Postives = 122/180 (67.78%), Query Frame = 1


Query: 6   SPFMTTPLPSLLLFIIIFNISTTFVVADLVQKACKKCEIDDPNINYNFCTSSFRAHSGSD 65
           S F  T LP  L F   F +       +L+   CKKC  DDPN++Y FC +S +A   S 
Sbjct: 4   STFSLTSLPLFLFFFFFFTLKA-ITAKNLIPDTCKKCVQDDPNLSYKFCVTSLQAAPNSS 63

Query: 66  STDLRKLGAISLSLIQRNLSSSFEYVEKLLQNKEIDSYKRVRLNDCLDVYSDAIVTVEEG 125
           S D+R+LG IS++LI++N++S+ +Y+++L++N+++D + R  L  CL++YSDAI  +++ 
Sbjct: 64  SADVRQLGIISMNLIRQNMTSTRQYIKQLMKNRKLDKFLRAYLEVCLELYSDAIPDIKQA 123

Query: 126 KKAYKEKHYDDANIKVSAVMDSARVCEDGFREKEGVSSPLTKWNKDMFQLAAIALSIINM 185
            + YK K Y DAN++V+ V D+   CEDGF E++G+ SPLTK N D  QL+AIALSII+M
Sbjct: 124 LRYYKAKQYRDANVRVTGVYDAPVTCEDGFHERKGLVSPLTKRNNDAVQLSAIALSIIDM 182

[Close]

BLAST of Cucsa.362300 vs. TAIR10
Match: AT5G46970.1 (AT5G46970.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 89.4 bits (220), Expect = 2.6e-18
Identity = 54/161 (33.54%), Postives = 82/161 (50.93%), Query Frame = 1


Query: 16  LLLFIIIFNISTTFVVAD-LVQKACKKCEIDDPNINYNFCTSSFRAHSGSDST-DLRKLG 75
           L  F + F +   F +A  L+Q  CKK    +P I+YNFC  S   +  S +   L +L 
Sbjct: 4   LFYFAMFFLLLNAFTIAQSLIQDFCKKAADKNPKIHYNFCVKSLEENPQSKTARSLDRLV 63

Query: 76  AISLSLIQRNLSSSFEYVEKLLQNKEIDSYKRVRLNDCLDVYSDAIVTVEEGKKAYKEKH 135
            +S        +S    V+K+L+    + Y    L DCL++YSDA  +++E     K + 
Sbjct: 64  MLSTKNAVSKTTSMKGIVDKILKENRFEMYSEKPLRDCLELYSDATNSLKEALTIIKSRD 123

Query: 136 YDDANIKVSAVMDSARVCEDGFRE-KEGVSSPLTKWNKDMF 174
           Y  AN+ +SA M +   CE GF+E K+ + SP TK N  +F
Sbjct: 124 YKTANVVISAAMGAPPSCEIGFKEGKKPLKSPFTKDNDVLF 164

[Close]

BLAST of Cucsa.362300 vs. TAIR10
Match: AT5G38610.1 (AT5G38610.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 89.0 bits (219), Expect = 3.5e-18
Identity = 55/183 (30.05%), Postives = 93/183 (50.82%), Query Frame = 1


Query: 16  LLLFIIIFNISTTFVVADLVQKACKKCEIDDPNINYNFCTSSFRAHSGS--DSTDLRKLG 75
           + LF ++  + +T   +D++ + CK C       +YNFC SS      +    T+L  L 
Sbjct: 10  IFLFFLVSQVKST---SDMIDQTCKSCAAKSTIFDYNFCVSSLNNSPIALPSPTNLSSLA 69

Query: 76  AISLSLIQRNLSSSFEYVEKLLQNKEIDSYKRVRLNDCLDVYSDAIVTVEEGKKAY-KEK 135
            + +     N +++   +++LL + +  S++   L DCL++Y DA   +EE  + +   K
Sbjct: 70  LVPMLQALDNATATASTIQQLLISDDDGSFRSACLRDCLELYEDATDRLEEAVRVFITRK 129

Query: 136 HYDDANIKVSAVMDSARVCEDGFREKE-------GV---SSPLTKWNKDMFQLAAIALSI 186
                N+ VSA M+SA  CE+GFRE++       GV   +SP+   N  +F+   IAL I
Sbjct: 130 ELGTVNVMVSAAMESAVTCENGFRERDDGDGGGGGVTTWTSPIGDENHKLFEFGQIALCI 189

[Close]

BLAST of Cucsa.362300 vs. TAIR10
Match: AT5G46940.1 (AT5G46940.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 87.8 bits (216), Expect = 7.7e-18
Identity = 49/172 (28.49%), Postives = 88/172 (51.16%), Query Frame = 1


Query: 17  LLFIIIFNISTTFVV--ADLVQKACKKCEIDDPNINYNFCTSSFRAH-SGSDSTDLRKLG 76
           LL+++ F + +  +     L++ +CKK     P   YN C +S   +     + DL  L 
Sbjct: 4   LLYLVTFFVLSNGLANGQTLIRNSCKKATATSPKFKYNLCVTSLETNPQAKTAKDLAGLV 63

Query: 77  AISLSLIQRNLSSSFEYVEKLLQNKEIDSYKRVRLNDCLDVYSDAIVTVEEGKKAYKEKH 136
             S        ++    V+K+++ K+++    + L DCL +Y+DAI ++ E     K ++
Sbjct: 64  MASTKNAVTKATTLKGTVDKIIKGKKVNKMTAMPLRDCLQLYTDAIGSLNEALAGVKSRN 123

Query: 137 YDDANIKVSAVMDSARVCEDGFREKEGVSSPLTKWNKDMFQLAAIALSIINM 186
           Y      +SA MD+   CE GF+E++   SP+TK N +++Q+  I L+  NM
Sbjct: 124 YPTVKTVLSAAMDTPSTCETGFKERK-APSPVTKENDNLYQMILIPLAFTNM 174

[Close]

BLAST of Cucsa.362300 vs. TAIR10
Match: AT5G46930.1 (AT5G46930.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 78.6 bits (192), Expect = 4.7e-15
Identity = 52/173 (30.06%), Postives = 87/173 (50.29%), Query Frame = 1


Query: 16  LLLFIIIFNISTTFVVADLVQKACKKCEIDDPNINYNFCTSSFRAHSGSDST-DLRKLGA 75
           L++F ++ N   T     L++ +CKK    DP + Y+FC  S   +  S +   L  L  
Sbjct: 7   LVMFFLLLNCFAT--AQSLIRDSCKKATTKDPKLKYDFCVKSLEENPQSKTAKSLEGLVF 66

Query: 76  ISLSLIQRNLSSSFEYVEKLLQNKEIDSYKRVR-LNDCLDVYSDAIVTVEEGKKAYKEKH 135
           +S        +S    V+K+L+    D Y+  R L DCL++Y++AI ++ +     K + 
Sbjct: 67  VSTKNAVSKTTSLKGMVDKILKE---DKYEVERPLLDCLELYTEAIDSLNQSLDTVKSRD 126

Query: 136 YDDANIKVSAVMDSARVCEDGF-REKEGVSSPLTKWNKDMFQLAAIALSIINM 186
           Y  A + +SA MD+   CE  F + K+ V SP TK N  +F +  I ++  +M
Sbjct: 127 YKTATMLMSAAMDAPGSCETKFTKRKKAVKSPFTKENDVLFYMVLIPIAFTSM 174

[Close]

BLAST of Cucsa.362300 vs. TAIR10
Match: AT5G46960.1 (AT5G46960.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 69.3 bits (168), Expect = 2.8e-12
Identity = 53/175 (30.29%), Postives = 85/175 (48.57%), Query Frame = 1


Query: 13  LPSLLLFIIIFNISTTFVVADLVQKACKKCEIDDPNINYNFCTSSFRAHSGSD-STDLRK 72
           L SL++F +  N   T     L+Q +CKK    DP ++Y+FC +S      S  +T L  
Sbjct: 4   LVSLVIFSLFLNGFAT--AQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLES 63

Query: 73  LGAISLSLIQRNLSSSFEYVEKLLQNKEI-DSYKRVRLNDCLDVYSDAIVTVEEGKKAYK 132
           L   S       +++    V + L+++   D  + ++L  CL  Y+DA   +       K
Sbjct: 64  LVLASTKTAAAKITNLKGIVAQDLKDQRYQDIVEDLKL--CLGFYNDANDDLTTALANIK 123

Query: 133 EKHYDDANIKVSAVMDSARVCEDGFREKEGVSSPLTKWNKDMFQLAAIALSIINM 186
            + Y  ANI +SA +D    CED F+E +  +SP+T  N  +F+   I L+  NM
Sbjct: 124 SRDYQGANINLSAALDVPGNCEDDFKEAK-KTSPITNENSILFKTILIPLAFTNM 173

[Close]

BLAST of Cucsa.362300 vs. NCBI nr
Match: gi|778711377|ref|XP_004145080.2| (PREDICTED: putative invertase inhibitor [Cucumis sativus])

HSP 1 Score: 372.5 bits (955), Expect = 4.4e-100
Identity = 186/187 (99.47%), Postives = 186/187 (99.47%), Query Frame = 1


Query: 1   MKPKFSPFMTTPLPSLLLFIIIFNISTTFVVADLVQKACKKCEIDDPNINYNFCTSSFRA 60
           MKPKFSPFMTTPLP LLLFIIIFNISTTFVVADLVQKACKKCEIDDPNINYNFCTSSFRA
Sbjct: 1   MKPKFSPFMTTPLPCLLLFIIIFNISTTFVVADLVQKACKKCEIDDPNINYNFCTSSFRA 60

Query: 61  HSGSDSTDLRKLGAISLSLIQRNLSSSFEYVEKLLQNKEIDSYKRVRLNDCLDVYSDAIV 120
           HSGSDSTDLRKLGAISLSLIQRNLSSSFEYVEKLLQNKEIDSYKRVRLNDCLDVYSDAIV
Sbjct: 61  HSGSDSTDLRKLGAISLSLIQRNLSSSFEYVEKLLQNKEIDSYKRVRLNDCLDVYSDAIV 120

Query: 121 TVEEGKKAYKEKHYDDANIKVSAVMDSARVCEDGFREKEGVSSPLTKWNKDMFQLAAIAL 180
           TVEEGKKAYKEKHYDDANIKVSAVMDSARVCEDGFREKEGVSSPLTKWNKDMFQLAAIAL
Sbjct: 121 TVEEGKKAYKEKHYDDANIKVSAVMDSARVCEDGFREKEGVSSPLTKWNKDMFQLAAIAL 180

Query: 181 SIINMHP 188
           SIINMHP
Sbjct: 181 SIINMHP 187

[Close]

BLAST of Cucsa.362300 vs. NCBI nr
Match: gi|659120446|ref|XP_008460198.1| (PREDICTED: putative invertase inhibitor [Cucumis melo])

HSP 1 Score: 332.8 bits (852), Expect = 3.9e-88
Identity = 163/179 (91.06%), Postives = 172/179 (96.09%), Query Frame = 1


Query: 9   MTTPLPSLLLFIIIFNISTTFVVADLVQKACKKCEIDDPNINYNFCTSSFRAHSGSDSTD 68
           MTTPLP LLLFI+IFNISTT VVADLVQK CKKCE DDPN+NYNFC SSFRAHSGSDSTD
Sbjct: 1   MTTPLPCLLLFIMIFNISTTIVVADLVQKTCKKCETDDPNVNYNFCISSFRAHSGSDSTD 60

Query: 69  LRKLGAISLSLIQRNLSSSFEYVEKLLQNKEIDSYKRVRLNDCLDVYSDAIVTVEEGKKA 128
           LRKLGAISLSLIQ+NLSSS EYVEKLLQNKEIDSY+RVRLNDCLDVYSDAIV VEEGKKA
Sbjct: 61  LRKLGAISLSLIQKNLSSSLEYVEKLLQNKEIDSYRRVRLNDCLDVYSDAIVNVEEGKKA 120

Query: 129 YKEKHYDDANIKVSAVMDSARVCEDGFREKEGVSSPLTKWNKDMFQLAAIALSIINMHP 188
           YKEKHYDDANIKVS+VMD+ARVCEDGFREKEGVSSPLTKWNKD+FQLAAIALSI+NM+P
Sbjct: 121 YKEKHYDDANIKVSSVMDAARVCEDGFREKEGVSSPLTKWNKDLFQLAAIALSIVNMYP 179

[Close]

BLAST of Cucsa.362300 vs. NCBI nr
Match: gi|590724254|ref|XP_007052414.1| (Plant invertase/pectin methylesterase inhibitor superfamily protein [Theobroma cacao])

HSP 1 Score: 181.0 bits (458), Expect = 1.9e-42
Identity = 93/182 (51.10%), Postives = 131/182 (71.98%), Query Frame = 1


Query: 9   MTTPLPSLLLFIIIFNISTTF---VVADLVQKACKKCEIDDPNINYNFCTSSFRAHSGSD 68
           M +  P L LF+  F  ++TF      DL+++ CKKC   DPN++YNFC +S +A   S 
Sbjct: 4   MLSSFPFLDLFLFFFIFTSTFHAVTSTDLIRETCKKCAGRDPNLSYNFCVTSLQAAPKSH 63

Query: 69  -STDLRKLGAISLSLIQRNLSSSFEYVEKLLQNKE-IDSYKRVRLNDCLDVYSDAIVTVE 128
            + DLR LG +S+ L++RNL+++  YVE+LL+NK+ +D + R  L+DC ++YSDAI + +
Sbjct: 64  CADDLRDLGMVSIRLVRRNLTNTRSYVEELLKNKKLVDPFVRSCLHDCFNLYSDAIPSTK 123

Query: 129 EGKKAYKEKHYDDANIKVSAVMDSARVCEDGFREKEGVSSPLTKWNKDMFQLAAIALSII 186
           E  + YK +HYDDANI VSAVMD+A  CEDGF+EKEGV SPLTK N + FQL+AI++SI+
Sbjct: 124 EAIEDYKSQHYDDANIDVSAVMDAATTCEDGFKEKEGVVSPLTKRNNNTFQLSAISISIV 183

[Close]

BLAST of Cucsa.362300 vs. NCBI nr
Match: gi|743787742|ref|XP_011031708.1| (PREDICTED: putative invertase inhibitor [Populus euphratica])

HSP 1 Score: 179.5 bits (454), Expect = 5.5e-42
Identity = 87/171 (50.88%), Postives = 124/171 (72.51%), Query Frame = 1


Query: 16  LLLFI-IIFNISTTFVVADLVQKACKKCEIDDPNINYNFCTSSFRAHSGSDSTDLRKLGA 75
           LLLF+ ++F+        +L+ + CKKC  +DPN++YNFC +S +A + S   +LR LG 
Sbjct: 9   LLLFLPLLFSTFHANTAQNLIPETCKKCAANDPNLSYNFCVTSLQASNRSQCDNLRGLGM 68

Query: 76  ISLSLIQRNLSSSFEYVEKLLQNKEIDSYKRVRLNDCLDVYSDAIVTVEEGKKAYKEKHY 135
           +S+ LI+ N++++  YV+ LL+NK++D + R  LNDCLD+YSDAI T+++    YK KHY
Sbjct: 69  MSIKLIKDNVTNTRNYVKSLLKNKKMDPFIRACLNDCLDLYSDAIPTLKQAMMDYKSKHY 128

Query: 136 DDANIKVSAVMDSARVCEDGFREKEGVSSPLTKWNKDMFQLAAIALSIINM 186
            DANI+V +V+D+A  CEDGF EKEG  SPLTK N D FQL+AIAL++INM
Sbjct: 129 KDANIEVGSVIDAATTCEDGFEEKEGAVSPLTKRNNDTFQLSAIALALINM 179

[Close]

BLAST of Cucsa.362300 vs. NCBI nr
Match: gi|566202453|ref|XP_006375099.1| (invertase/pectin methylesterase inhibitor family protein [Populus trichocarpa])

HSP 1 Score: 178.7 bits (452), Expect = 9.4e-42
Identity = 87/171 (50.88%), Postives = 125/171 (73.10%), Query Frame = 1


Query: 16  LLLFI-IIFNISTTFVVADLVQKACKKCEIDDPNINYNFCTSSFRAHSGSDSTDLRKLGA 75
           LLLF+ ++F      +  +L+ + CKKC  +DPN++YNFC +S +A + S   +LR LG 
Sbjct: 9   LLLFLPLLFCTFHANIAQNLIPETCKKCAANDPNLSYNFCVTSLQASNRSQCDNLRGLGM 68

Query: 76  ISLSLIQRNLSSSFEYVEKLLQNKEIDSYKRVRLNDCLDVYSDAIVTVEEGKKAYKEKHY 135
           +S+ LI+ N++++  YV+ LL+NK++D + R  LNDCLD+YSDAI T+++    YK KHY
Sbjct: 69  MSIKLIKYNVTNTRHYVKNLLKNKKMDPFIRACLNDCLDLYSDAIPTLKQAMIDYKSKHY 128

Query: 136 DDANIKVSAVMDSARVCEDGFREKEGVSSPLTKWNKDMFQLAAIALSIINM 186
            DANI+VS+V+D+A  CEDGF +KEG  SPLTK N D FQL+AIAL++INM
Sbjct: 129 KDANIEVSSVIDAATTCEDGFEDKEGAVSPLTKRNNDTFQLSAIALALINM 179

The following BLAST results are available for this feature:

BLAST of Cucsa.362300 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PLA1_PLAAC	1.4e-29	40.80	Putative invertase inhibitor OS=Platanus acerifolia PE=1 SV=1	[more]

BLAST of Cucsa.362300 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K9X7_CUCSA	3.1e-100	99.47	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G080360 PE=4 SV=1	[more]
A0A061DWX5_THECC	1.3e-42	51.10	Plant invertase/pectin methylesterase inhibitor superfamily protein OS=Theobroma...	[more]
B9MVC0_POPTR	6.5e-42	50.88	Invertase/pectin methylesterase inhibitor family protein OS=Populus trichocarpa ...	[more]
A0A0D2PD74_GOSRA	4.7e-40	48.17	Uncharacterized protein OS=Gossypium raimondii GN=B456_004G168200 PE=4 SV=1	[more]
M5X7H8_PRUPE	1.4e-39	45.00	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppb019226mg PE=4 SV=1	[more]

BLAST of Cucsa.362300 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G46970.1	2.6e-18	33.54	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G38610.1	3.5e-18	30.05	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46940.1	7.7e-18	28.49	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46930.1	4.7e-15	30.06	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46960.1	2.8e-12	30.29	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

BLAST of Cucsa.362300 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778711377\|ref\|XP_004145080.2\|	4.4e-100	99.47	PREDICTED: putative invertase inhibitor [Cucumis sativus]	[more]
gi\|659120446\|ref\|XP_008460198.1\|	3.9e-88	91.06	PREDICTED: putative invertase inhibitor [Cucumis melo]	[more]
gi\|590724254\|ref\|XP_007052414.1\|	1.9e-42	51.10	Plant invertase/pectin methylesterase inhibitor superfamily protein [Theobroma c...	[more]
gi\|743787742\|ref\|XP_011031708.1\|	5.5e-42	50.88	PREDICTED: putative invertase inhibitor [Populus euphratica]	[more]
gi\|566202453\|ref\|XP_006375099.1\|	9.4e-42	50.88	invertase/pectin methylesterase inhibitor family protein [Populus trichocarpa]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR006501	Pectinesterase_inhib_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0004857	enzyme inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.362300.1	Cucsa.362300.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	GENE3D	G3DSA:1.20.140.40		coord: 33..185 score: 2.2
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 33..182 score: 1.0
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 30..182 score: 4.2
IPR006501	Pectinesterase inhibitor domain	TIGRFAMs	TIGR01614	TIGR01614	coord: 15..185 score: 1.3
IPR006501	Pectinesterase inhibitor domain	unknown	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 33..185 score: 1.57
None	No IPR available	PANTHER	PTHR31707	FAMILY NOT NAMED	coord: 4..185 score: 3.2
None	No IPR available	PANTHER	PTHR31707:SF53	SUBFAMILY NOT NAMED	coord: 4..185 score: 3.2

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.362300	Carg14888	Silver-seed gourd	carcgyB0695

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.362300	Melon (DHL92) v3.6.1	cgymedB586
Cucsa.362300	Melon (DHL92) v3.6.1	cgymedB592
Cucsa.362300	Silver-seed gourd	carcgyB0271
Cucsa.362300	Silver-seed gourd	carcgyB1079
Cucsa.362300	Silver-seed gourd	carcgyB1194
Cucsa.362300	Cucumber (Chinese Long) v3	cgycucB553
Cucsa.362300	Cucumber (Chinese Long) v3	cgycucB556
Cucsa.362300	Watermelon (97103) v2	cgywmbB622
Cucsa.362300	Watermelon (97103) v2	cgywmbB625
Cucsa.362300	Wax gourd	cgywgoB724
Cucsa.362300	Wax gourd	cgywgoB728
Cucsa.362300	Cucumber (Gy14) v1	cgycgyB130
Cucsa.362300	Cucurbita maxima (Rimu)	cgycmaB0998
Cucsa.362300	Cucurbita maxima (Rimu)	cgycmaB1007
Cucsa.362300	Cucurbita maxima (Rimu)	cgycmaB1008
Cucsa.362300	Cucurbita moschata (Rifu)	cgycmoB0992
Cucsa.362300	Cucurbita moschata (Rifu)	cgycmoB1007
Cucsa.362300	Cucurbita moschata (Rifu)	cgycmoB0999
Cucsa.362300	Cucurbita moschata (Rifu)	cgycmoB1008
Cucsa.362300	Wild cucumber (PI 183967)	cgycpiB537
Cucsa.362300	Wild cucumber (PI 183967)	cgycpiB540
Cucsa.362300	Cucumber (Chinese Long) v2	cgycuB509
Cucsa.362300	Cucumber (Chinese Long) v2	cgycuB511
Cucsa.362300	Melon (DHL92) v3.5.1	cgymeB586
Cucsa.362300	Melon (DHL92) v3.5.1	cgymeB594
Cucsa.362300	Watermelon (Charleston Gray)	cgywcgB617
Cucsa.362300	Watermelon (Charleston Gray)	cgywcgB620
Cucsa.362300	Watermelon (97103) v1	cgywmB663
Cucsa.362300	Watermelon (97103) v1	cgywmB666
Cucsa.362300	Cucurbita pepo (Zucchini)	cgycpeB0945
Cucsa.362300	Cucurbita pepo (Zucchini)	cgycpeB0947
Cucsa.362300	Cucurbita pepo (Zucchini)	cgycpeB0960
Cucsa.362300	Cucurbita pepo (Zucchini)	cgycpeB0965
Cucsa.362300	Cucurbita pepo (Zucchini)	cgycpeB0969
Cucsa.362300	Bottle gourd (USVL1VR-Ls)	cgylsiB583
Cucsa.362300	Bottle gourd (USVL1VR-Ls)	cgylsiB585